Advanced biology最新文献

The Immobilization of Hyaluronic Acid in 3D Hydrogel Scaffolds Modulates Macrophage Polarization. 透明质酸在三维水凝胶支架中的固定化调节巨噬细胞极化。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-10-13 DOI: 10.1002/adbi.202400682

Tiah Cl Oates, Jasmin Boyd, Louise Dolan, C de Kergariou, Jingwen Zhu, Ash Toye, Adam W Perriman, Asme Boussahel

{"title":"The Immobilization of Hyaluronic Acid in 3D Hydrogel Scaffolds Modulates Macrophage Polarization.","authors":"Tiah Cl Oates, Jasmin Boyd, Louise Dolan, C de Kergariou, Jingwen Zhu, Ash Toye, Adam W Perriman, Asme Boussahel","doi":"10.1002/adbi.202400682","DOIUrl":"https://doi.org/10.1002/adbi.202400682","url":null,"abstract":"Macrophages are key modulators of immunity, tissue homeostasis and disease development. As our understanding of macrophage biology and their tissue-specific behaviors grows the necessity to model macrophages within a 3D biomimetic environment becomes increasingly apparent. Numerous hydrogels are developed and explored for this purpose, extracellular matrix (ECM) mimicking hydrogels gaining special interest. In this study, the use of such a hydrogel composed of collagen and hyaluronic acid (HA), two of the major ECM components, is presented for the 3D culture of macrophages to model their role in different tissues and diseases. The ability to tailor the mechanical properties of the hydrogel through formulation modulation is demonstrated. Human macrophages retain morphology, viability, and expression of key cell surface markers when 3D cultured within the hydrogel. Interestingly, it is demonstrate in this work, that independent of mechanical properties, by adjusting the composition of the hydrogel, specifically HA molecular weight, steers macrophage polarization toward either a pro-inflammatory or anti-inflammatory phenotype. This HA-dependent modulation of macrophage behavior is nullified if the HA is chemically crosslinked, shedding light on the impact of one of the most commonly used preparation methods for collagen-HA hydrogels on macrophage behavior.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00682"},"PeriodicalIF":2.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amide-Linked Local Anesthetics Alter Tumor Biology in a Complex Human Tissue Model of Non-Small Cell Lung Adenocarcinoma. 酰胺连接局部麻醉剂改变非小细胞肺腺癌复杂人体组织模型的肿瘤生物学。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-30 DOI: 10.1002/adbi.202500280

Juliane Krömer, Sebastian Krämer, Ngoc Anh Hoang, Doreen Sittig, Isabella Metelmann, Uta-Carolin Pietsch, Sebastian N Stehr, Sonja Kallendrusch, Tobias Piegeler

{"title":"Amide-Linked Local Anesthetics Alter Tumor Biology in a Complex Human Tissue Model of Non-Small Cell Lung Adenocarcinoma.","authors":"Juliane Krömer, Sebastian Krämer, Ngoc Anh Hoang, Doreen Sittig, Isabella Metelmann, Uta-Carolin Pietsch, Sebastian N Stehr, Sonja Kallendrusch, Tobias Piegeler","doi":"10.1002/adbi.202500280","DOIUrl":"https://doi.org/10.1002/adbi.202500280","url":null,"abstract":"Amide local anesthetics (LA) affect tumor burden in various preclinical studies, possibly via their anti-inflammatory properties. However, a translation into clinical evidence is still lacking. Here, effects of LA at clinically relevant concentrations are assessed using a human ex vivo tumor model of patient-derived tumor slice cultures from nine patients with non-small cell lung cancer. Tumors are cultivated for four days and treated with LA in absence/presence of cisplatin. Tumor cell proliferation and apoptosis as well as expression of intercellular adhesion molecule-1 and macrophage polarization are assessed using immunofluorescent imaging. Tumor specimens are considered to be \"Responders\", when a change in proliferation and/or apoptosis by >50% compared to untreated slices occurred. Five of nine samples are \"Responders\", in which the LA exerted effects comparable to cisplatin. Even at clinically relevant concentrations of LA, a strong anti-tumoral effect is observable in patient-derived tumor slice cultures with complex structures of the tumor microenvironment highlighting the LA effect on the tumor itself and its surroundings, without any interference by other leukocytes or neuronal stimulation. The diverse reaction to LA treatment also emphasizes the importance of biomarkers to determine the tumor phenotypes, which may benefit from LA treatment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00280"},"PeriodicalIF":2.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glaucocalyxin A Induces Cytotoxicity in Renal Cancer Cells via ROS-Mediated Autophagy by Direct Targeting of Antioxidant Enzymes PRDX1 and PRDX2. 青萼藻素A直接靶向抗氧化酶PRDX1和PRDX2，通过ros介导的自噬诱导肾癌细胞毒性

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-30 DOI: 10.1002/adbi.202500031

Yaping Niu, Jinhuan Ou, Xiaoru Zhong, Piao Luo, Junhui Chen, Ashok Iyaswamy, Haibo Tong, Zhou Zhu, Peng Chen, Xu Wei, Wei Zhang, Hualin Ma, Yulin Feng, Chuanbin Yang, Jigang Wang

{"title":"Glaucocalyxin A Induces Cytotoxicity in Renal Cancer Cells via ROS-Mediated Autophagy by Direct Targeting of Antioxidant Enzymes PRDX1 and PRDX2.","authors":"Yaping Niu, Jinhuan Ou, Xiaoru Zhong, Piao Luo, Junhui Chen, Ashok Iyaswamy, Haibo Tong, Zhou Zhu, Peng Chen, Xu Wei, Wei Zhang, Hualin Ma, Yulin Feng, Chuanbin Yang, Jigang Wang","doi":"10.1002/adbi.202500031","DOIUrl":"https://doi.org/10.1002/adbi.202500031","url":null,"abstract":"Glaucocalyxin A (GLA), a bioactive diterpenoid from the medicinal plant Rabdosia japonica, demonstrates potent antitumor activity, yet its molecular mechanisms in renal cell carcinoma (RCC) remain elusive. Here, GLA is reported to trigger cytotoxicity in RCC cells through reactive oxygen species (ROS) overaccumulation. Mechanistically, ROS surge activates autophagy, and pharmacological or genetic autophagy inhibition significantly rescues GLA-induced cell death, indicating autophagy acts as a pro-death effector in this context. Employing activity-based protein profiling (ABPP) coupled with proteomic analysis, peroxiredoxins PRDX1/2 are identified as direct covalent targets of GLA. Functional validation reveals that PRDX1/2 overexpression mitigates GLA-mediated apoptosis, establishing their role as critical redox sensors governing cell fate. The findings delineate a ROS-autophagy-apoptosis axis driven by PRDX1/2 targeting, positioning GLA as a novel therapeutic scaffold for RCC treatment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00031"},"PeriodicalIF":2.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

snoRNP RRP9 Promotes Prostate Cancer Cell Proliferation and Migration via SQSTM1. snoRNP RRP9通过SQSTM1促进前列腺癌细胞增殖和迁移

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-24 DOI: 10.1002/adbi.202500182

Butang Li, Lihui Shen, Hui Huang, Kai Shen, Xiaorong Wu, Chenfei Chi, Jiahua Pan

{"title":"snoRNP RRP9 Promotes Prostate Cancer Cell Proliferation and Migration via SQSTM1.","authors":"Butang Li, Lihui Shen, Hui Huang, Kai Shen, Xiaorong Wu, Chenfei Chi, Jiahua Pan","doi":"10.1002/adbi.202500182","DOIUrl":"https://doi.org/10.1002/adbi.202500182","url":null,"abstract":"Small nucleolar RNAs (snoRNAs)-60-300 nucleotide non-coding RNAs are associated with adverse clinical outcomes in cancer patients. However, information on the role of snoRNAs and associated small nuclear ribonucleoprotein (snoRNPs) in prostate cancer (PCa) remains scarce. Here, the contribution of the snoRNP U3 snoRNA-interacting protein 2 (RRP9) in PCa pathogenesis is investigated. A combination of three different shRNAs is employed to knockdown RRP9 in the PCa cell lines DU-145 and PC-3. Cell proliferation is evaluated by seeding cells into a 96-well plates and monitoring daily. Cell migration is evaluated by scratch and Transwell assays. FLAG-RRP9 pull-down, MALDI-TOF/TOF, and co-immunoprecipitation assays are conducted to identify RRP9 binding partners in DU-145 cells. In vitro cell proliferation and migration, as well as in vivo tumor growth, are suppressed following RRP9 knockdown. Pull-down and MALDI-TOF/TOF analyses identified five putative RRP9 binding partners, and co-immunoprecipitation validated that RRP9 interacts with the scaffolding hub protein Sequestome-1 (SQSTM1, p62). Interestingly, SQSTM1 overexpression rescued the anti-growth/migration effects of RRP9 knockdown. This study unveiled a novel oncogenic role for the RRP9-SQSTM1 axis in PCa cells. RRP9 is a snoRNP that binds to SQSTM1, thereby promoting PCa cell proliferation and migration. Targeting the RRP9-SQSTM1 axis may be a viable therapeutic strategy for PCa.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00182"},"PeriodicalIF":2.6,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SOX4 Regulates Thermogenesis in Brown Adipose Tissue via Independent Complexes with EBF2 and PPARγ. SOX4通过与EBF2和PPARγ的独立复合物调节棕色脂肪组织的产热作用。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-22 DOI: 10.1002/adbi.202500224

Shuai Wang, Ting He, Tong Fu, Yu Zhu, Yixin Wei, Wenlong Xie, Huanming Shen, Ya Luo, Boan Li, Huiling Guo, Weihua Li

{"title":"SOX4 Regulates Thermogenesis in Brown Adipose Tissue via Independent Complexes with EBF2 and PPARγ.","authors":"Shuai Wang, Ting He, Tong Fu, Yu Zhu, Yixin Wei, Wenlong Xie, Huanming Shen, Ya Luo, Boan Li, Huiling Guo, Weihua Li","doi":"10.1002/adbi.202500224","DOIUrl":"https://doi.org/10.1002/adbi.202500224","url":null,"abstract":"Brown adipose tissue (BAT) is crucial for maintaining whole-body metabolic homeostasis and combating obesity and metabolic disorders. SOX4 collaborates with EBF2 to promote the expression of thermogenic genes in BAT, but it is unclear whether there are mechanisms independent of this regulation. However, it is found that SOX4 can directly interact with the promoter regions of thermogenic genes, thereby activating their expression. Simultaneously, early B cell factor 2 (EBF2) and peroxisome proliferator-activated receptor-γ (PPARγ) can independently interact with SOX4, forming two distinct complexes that promote the expression of thermogenic genes. Phenotypically, the deletion of SOX4 in BAT of mice (Ucp1Cre+-Sox4f/f (Sox4-BKO)) leads to the downregulation of thermogenic and oxidative phosphorylation genes, as well as a reduction in mitochondrial numbers. Furthermore, Sox4-BKO mice are more susceptible to obesity, glucose intolerance, and insulin resistance when subjected to a high-fat diet (HFD). Consistently, the loss of SOX4 results in increased cellular triglyceride content and reduced expression levels of thermogenic genes in vitro. Together, a novel mechanism by which SOX4 regulates thermogenesis in BAT is elucidated, offering a promising strategy to address obesity and metabolic disorders.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00224"},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Stat3 in Prx1⁺ Progenitors Impairs Molar Root Development. Prx1+祖细胞中Stat3的缺失会损害臼齿根的发育。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-12 DOI: 10.1002/adbi.202500329

Xin Feng, Wangyu Luo, Yichen Yao, Lichieh Lin, Laiting Chan, Jiarui Lu, Zijing Huang, Jingyi Feng, Le Zhao, Xiaolei Zhang, Liu Yang

{"title":"Loss of Stat3 in Prx1+ Progenitors Impairs Molar Root Development.","authors":"Xin Feng, Wangyu Luo, Yichen Yao, Lichieh Lin, Laiting Chan, Jiarui Lu, Zijing Huang, Jingyi Feng, Le Zhao, Xiaolei Zhang, Liu Yang","doi":"10.1002/adbi.202500329","DOIUrl":"https://doi.org/10.1002/adbi.202500329","url":null,"abstract":"Signal Transducer and Activator of Transcription 3 (Stat3) acts as a central transcriptional modulator coordinating cellular proliferation, survival, apoptosis, vascularization, immune regulation, and migratory processes. Human Stat3 deficiency triggers Hyper-IgE syndrome, associated with immune dysregulation, osseous defects, and dental malformations. This study employs genetically engineered murine models to dissect Stat3's mechanistic role within mesenchymal progenitor cells during molar root formation and periodontal tissue maturation. Conditional Stat3 knockout mice (Prx1-Cre; Stat3f/f) are generated. Comparative assessments of mandibular first molar root development between Stat3 CKO and wild-type cohorts are performed through histomorphometric evaluation, micro-computed tomography, cellular proliferation assays (Ki67/BrdU), and transcriptome sequencing. Stat3 ablation causes marked morphological defects in first molars, featuring reduced root length and elevated crown-root proportion. The periodontal ligament (PDL) at the distal root exhibits diminished width in mutants. Alveolar bone displays suppressed expression of osteogenic markers (Runx2, Col1a1, Ocn), accompanied by decreased Ki67+ and BrdU+ cell populations in the PDL. Stat3 critically regulates mandibular first molar and alveolar bone morphogenesis. Conditional ablation of Stat3 disrupts the osteogenic capacity of Prx1+ mesenchymal progenitors, as evidenced across in vivo and in vitro models.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00329"},"PeriodicalIF":2.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RRP9 Promotes Esophageal Squamous Cell Carcinoma Progression through E2F1 Transcriptional Regulation of CDK1. RRP9通过E2F1转录调控CDK1促进食管鳞状细胞癌进展。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-12 DOI: 10.1002/adbi.202500220

Gang He, Yilong Wan, Yanbo Zhu, Bo Peng, Shengxiang Shao, Xinyi Zou, Zhenyu Han, Jiaxi Li, Sheng Ju, Xin Tong, Jun Zhao, Hansi Liang, Liuqing Zhang, Jiashi Xiong, Dong Jiang

{"title":"RRP9 Promotes Esophageal Squamous Cell Carcinoma Progression through E2F1 Transcriptional Regulation of CDK1.","authors":"Gang He, Yilong Wan, Yanbo Zhu, Bo Peng, Shengxiang Shao, Xinyi Zou, Zhenyu Han, Jiaxi Li, Sheng Ju, Xin Tong, Jun Zhao, Hansi Liang, Liuqing Zhang, Jiashi Xiong, Dong Jiang","doi":"10.1002/adbi.202500220","DOIUrl":"https://doi.org/10.1002/adbi.202500220","url":null,"abstract":"Esophageal cancer is a major disease that seriously threatens human health. Ribosomal RNA biogenesis is implicated in tumorigenesis, while the small nucleolar RNAs (snoRNAs) are responsible for post-transcriptional modifications of ribosomal RNAs during biogenesis, which are identified as potential markers of various cancers. The clinical significance, biological behavior, and potential molecular mechanism of the nucleolar small nuclear ribonucleoprotein RRP9 in esophageal squamous cell carcinoma (ESCC), which is a major pathological type of esophageal cancer, are investigated in this study. It is found that RRP9 is abnormally highly expressed in ESCC tissues and is closely associated with poor prognosis. Furthermore, it is found that RRP9 could regulate the cell cycle protein-dependent kinase CDK1 to promote ESCC progression in vitro and in vivo. Mechanistically, RRP9 promotes ESCC progression through enhancing the E2F1-mediated transcriptional regulation of CDK1. Collectively, the results defined RRP9 as an important tumor driver in ESCC that acted by activating oncogenic signaling by the E2F1-CDK1 pathway, and suggested RRP9 as a candidate therapeutic target for ESCC.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00220"},"PeriodicalIF":2.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Insulin-Like Growth Factor Mimetic Materials. 胰岛素样生长因子模拟材料的研究进展。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-09-04 DOI: 10.1002/adbi.202500327

Abhishek Roy, Joseph B Dodd-O, Bobak Shadpoor, Siya K Patel, Gelavizh Gharati, Marleen Hanna, Abdul Lateef-Fnu, Corey Heffernan, Vivek A Kumar

{"title":"Development of Insulin-Like Growth Factor Mimetic Materials.","authors":"Abhishek Roy, Joseph B Dodd-O, Bobak Shadpoor, Siya K Patel, Gelavizh Gharati, Marleen Hanna, Abdul Lateef-Fnu, Corey Heffernan, Vivek A Kumar","doi":"10.1002/adbi.202500327","DOIUrl":"https://doi.org/10.1002/adbi.202500327","url":null,"abstract":"Growth factors play a crucial role in regulating cellular processes such as proliferation, differentiation, and survival. Their activities are tightly modulated to ensure proper physiological functioning, with dysregulation often contributing to disease pathogenesis. Among these, the insulin-like growth factor (IGF) system that encompasses IGF-1 and IGF-receptor binding proteins is pivotal in maintaining overall cellular health by regulating growth, repair, and metabolic regulation. Capitalizing on its pro-mitogenic effects, translational studies have focused efforts on developing therapeutics based on IGF-1 for age-related muscle loss, metabolic disorders, or cardiovascular diseases. Mimetic peptide design has emerged as an innovative approach to overcoming limitations of direct IGF-1 therapy, focusing on structural optimization to enhance bioavailability, stability, and receptor specificity. Herein, the development of IGF-1 mimics and their potential clinical applications are reviewed. Their design and molecular properties, including structural considerations and mechanisms of action, are described. In vitro and in vivo approaches analyzed to provide insights into their pharmacokinetics, therapeutic efficacy, and safety profiles in animal models will be delved into. These preclinical studies shed light on the advantages of IGF-1 mimics, such as bioavailability, stability, and delivery, as well as the limitations, including potential immunogenicity.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00327"},"PeriodicalIF":2.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illuminating New Frontiers: Exploring the Photosensitizing Potential of Passiflora Species in Combating Methicillin-Resistant Staphylococcus aureus (MRSA) and Their Infection in Senescent Mice. 照亮新领域：探索西番莲属植物对抗耐甲氧西林金黄色葡萄球菌（MRSA）及其在衰老小鼠中的感染的光敏潜力。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-08-29 DOI: 10.1002/adbi.202500254

Caroline Vieira Gonçalves, Maria Poliana Leite Galantini, Igor Pereira Ribeiro Muniz, Paulo Henrique Bispo Lima, Israel Souza Ribeiro, Maria Eduarda Santos de Oliveira, Caio Oliveira Lopes de Magalhães, Maria Elisa Santos Flores, Samara Lopes de Oliveira, Catarina Silva Guimarães, Paulinne Moreira Lima, Luísa Carregosa Santos, Daiana Silva Lopes, Juliano Geraldo Amaral, Robson Amaro Augusto da Silva

{"title":"Illuminating New Frontiers: Exploring the Photosensitizing Potential of Passiflora Species in Combating Methicillin-Resistant Staphylococcus aureus (MRSA) and Their Infection in Senescent Mice.","authors":"Caroline Vieira Gonçalves, Maria Poliana Leite Galantini, Igor Pereira Ribeiro Muniz, Paulo Henrique Bispo Lima, Israel Souza Ribeiro, Maria Eduarda Santos de Oliveira, Caio Oliveira Lopes de Magalhães, Maria Elisa Santos Flores, Samara Lopes de Oliveira, Catarina Silva Guimarães, Paulinne Moreira Lima, Luísa Carregosa Santos, Daiana Silva Lopes, Juliano Geraldo Amaral, Robson Amaro Augusto da Silva","doi":"10.1002/adbi.202500254","DOIUrl":"https://doi.org/10.1002/adbi.202500254","url":null,"abstract":"Antimicrobial Photodynamic Therapy (aPDT) has become a potential alternative for treating multidrug-resistant bacterial skin infections, such as those caused by methicillin-resistant Staphylococcus aureus (MRSA), which are at high risk in aging individuals. One of the main components of aPDT is an agent known as a photosensitizer (PS). Some plants with high flavonoid content are reported as PS. In the genus Passiflora, flavonoids are predominant, but their photosensitizing activity has yet to be described. This study investigates the photosensitizing potential of extracts from Passiflora edulis, Passiflora alata, and Passiflora cincinnata. The butanolic fraction of P. cincinnata undergoes in vivo evaluation against intradermal MRSA infection in a senescent murine model (C57BL/6). In vitro assays determine the photoactivatable concentrations and their cytotoxicity. In vivo, MRSA-infected mice are divided into control, P. cincinnata-treated, and aPDT-treated groups. Subsequent assessments include cytokine levels, bacterial load, and cellular infiltrate in the ear. The P. cincinnata-treated group exhibits improved bacterial control, reduced leukocyte infiltration, and less weight loss. The aPDT group demonstrates a unique cytokine correlation profile, featuring more negative correlations among pro-inflammatory cytokines and interleukin-10. P. cincinnata emerges as an effective photosensitizer for aPDT in a senescent model and highlights the potential of underexplored plant-derived photosensitizers.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00254"},"PeriodicalIF":2.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detecting Dengue in Flight: Leveraging Machine Learning to Analyze Mosquito Flight Patterns for Infection Detection. 在飞行中检测登革热：利用机器学习分析蚊子飞行模式进行感染检测。

IF 2.6 3区生物学

Advanced biology Pub Date : 2025-08-28 DOI: 10.1002/adbi.202400847

Nouman Javed, Adam J López-Denman, Prasad N Paradkar, Asim Bhatti

{"title":"Detecting Dengue in Flight: Leveraging Machine Learning to Analyze Mosquito Flight Patterns for Infection Detection.","authors":"Nouman Javed, Adam J López-Denman, Prasad N Paradkar, Asim Bhatti","doi":"10.1002/adbi.202400847","DOIUrl":"https://doi.org/10.1002/adbi.202400847","url":null,"abstract":"With the growing global threat of mosquito-borne diseases, there is an urgent need for faster, automated methods to assess disease load in mosquitoes and predict outbreaks. Current surveillance relies on manual mosquito traps and labor-intensive lab tests like polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), which are time-consuming and resource-intensive. In this study, machine learning algorithms are applied to detect dengue-infected mosquitoes based on their 3D flight patterns. Using a convolutional neural network (CNN) and cubic spline interpolation, mosquito flight trajectories are tracked, followed by classification with models including CNN, eXtreme Gradient Boosting (XGBoost), Adaptive Boosting (AdaBoost), Random Forest, Decision Tree, Naive Bayes, Logistic Regression, Multi-Layer Perceptron (MLP), and a hybrid CNN + XGBoost model. The 5-fold cross-validation results showed that XGBoost achieved the highest mean accuracy (81.43%), while Random Forest has shown the best mean F1 Score (82.80%). Some validation folds demonstrated outstanding performance, with AdaBoost reaching 95.85% accuracy and Random Forest achieving 97.77% recall in Fold 1. The study also analyzed the impact of flight sequence size on models' performance, observing that longer sequences provided more accurate predictions. This approach offers a faster and more efficient method for assessing infection status, supporting real-time vector monitoring, and improving early disease outbreak detection.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00847"},"PeriodicalIF":2.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0