Advanced biology最新文献

Enteroendocrine Cells Sense Sucrose and Alter Enteric Neuron Excitability via Insulin Signaling.

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-20 DOI: 10.1002/adbi.202300566

Jessica R Snyder, Minhal Ahmed, Sukhada Bhave, Ryo Hotta, Ryan A Koppes, Allan M Goldstein, Abigail N Koppes

{"title":"Enteroendocrine Cells Sense Sucrose and Alter Enteric Neuron Excitability via Insulin Signaling.","authors":"Jessica R Snyder, Minhal Ahmed, Sukhada Bhave, Ryo Hotta, Ryan A Koppes, Allan M Goldstein, Abigail N Koppes","doi":"10.1002/adbi.202300566","DOIUrl":"https://doi.org/10.1002/adbi.202300566","url":null,"abstract":"Neurosensory circuits of the gastrointestinal tract sense microbial and nutrient changes in the gut; however, studying these circuits in vivo is hindered by invasive techniques and ethical concerns. Here, an in vitro model of enteroendocrine cells (EECs) and calcium reporting enteric neurons (ENs) is established and validated for functional signaling. Both mechanical and sucrose stimulation of co-cultures increased the percentage of neurons undergoing a calcium flux, indicating an action potential. Neuronal activation is blocked with either a piezo or insulin receptor blocker. At baseline, a flow only stimulus elicited 51.9% of neurons to activate in co-culture, which is decreased to 15.1% with a piezo blocker. Piezo blocked and sucrose stimulated EECs increased neuronal activation to 43.9%, and an insulin blocker reduced response to 12.4%. Since a cell line is used to model the EEC in the previous experiments, primary rat duodenal epithelium enriched for EECs are also stimulated and found to produced measurable insulin. This work shows the ability of EECs to produce insulin and for ENs to sense insulin. These results inspire further work on how insulin production outside the pancreas effects diabetes, insulin as a neurotransmitter, and exploration of additional nutritional and microbiotic stimuli on enteroendocrine-to-neuronal signaling.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2300566"},"PeriodicalIF":3.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Potential of Natural Resources-Derived Therapeutics for Improved Malaria Management: Computational to Experimental Studies.

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-19 DOI: 10.1002/adbi.202400282

Michael P Okoh, Maxwell O Egua, Lukman A Alli, Damayanthi Dalu, Rohit Gundamaraju, Rajeev K Singla, Bairong Shen

{"title":"Unveiling the Potential of Natural Resources-Derived Therapeutics for Improved Malaria Management: Computational to Experimental Studies.","authors":"Michael P Okoh, Maxwell O Egua, Lukman A Alli, Damayanthi Dalu, Rohit Gundamaraju, Rajeev K Singla, Bairong Shen","doi":"10.1002/adbi.202400282","DOIUrl":"https://doi.org/10.1002/adbi.202400282","url":null,"abstract":"Malaria kills millions of people annually, and it is one of the major causes of preventable mortality in the world. Of the different plasmodium species that induce malaria, Plasmodium falciparum and Plasmodium vivax account for the most severe form of malarial disease in humans. This review focuses on understanding preventive measures, mutation-based disease evolution, malaria-related biomarkers, and potential plant bioactive components for the treatment and management of malaria. The burden of malaria drug resistance has made it necessary for scientists to focus on alternative therapeutics, with particular interests in those involving plant-based bioactive components that could mediate biochemical pathways, consisting of metabolic interactions essential for parasitic inhibition. To avoid artefacts or false positives, these bioactive components from plant sources are further filtered using the \"pan-assay-interfering compounds\" (PAINS) tool. This review discussed the history of malaria treatment, current treatment options, malaria preventive measures, and challenges associated with current treatment strategies. Additionally, this work discusses the barriers while developing drugs from phytochemicals and the steps needed to accelerate the development of new antimalarial from the lead compounds.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400282"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Granulocyte Colony Stimulating Factor Enhances Decidualization Process of Endometrial Stromal Cells Through STAT3/HOXA10 Axis. 粒细胞集落刺激因子通过 STAT3/HOXA10 轴增强子宫内膜基质细胞的蜕膜化过程

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-18 DOI: 10.1002/adbi.202400279

Huakun Zhang, Zhengzhong Wu, Ningjie Yang, Shuhua Wu, Jing Fan, Ping Wang, Xuemei Li

{"title":"Granulocyte Colony Stimulating Factor Enhances Decidualization Process of Endometrial Stromal Cells Through STAT3/HOXA10 Axis.","authors":"Huakun Zhang, Zhengzhong Wu, Ningjie Yang, Shuhua Wu, Jing Fan, Ping Wang, Xuemei Li","doi":"10.1002/adbi.202400279","DOIUrl":"https://doi.org/10.1002/adbi.202400279","url":null,"abstract":"Background: Recurrent implantation failure (RIF) is characterized by the repeated failure of implantation, often linked to impaired endometrial receptivity. This study investigates how granulocyte colony-stimulating factor (G-CSF) promotes endometrial stromal cell decidualization.Methods: THESCs (human telomerase reverse transcriptase-immortalized endometrial stromal cells) were used as an in vitro cell model to induce decidualization. The effects of G-CSF on the expression of decidualization genes and apoptosis during decidualization were examined. Additionally, a chemical inhibitor of signal transducer and activator of transcription 3 (STAT3) and the small interfering RNA (siRNA) targeting Homeobox A10 (Hoxa10) were employed to explore the involvement of the STAT3/HOXA10 axis in the action of G-CSF.Results: G-CSF promoted decidualization markers expression and suppressed apoptosis in THESCs Treatment with G-CSF enhanced STAT3 activation during decidualization induction. STAT3 inhibition diminished the effects of G-CSF on decidualization marker expression and apoptosis suppression. Furthermore, it was demonstrated that G-CSF increased Hoxa10 expression in a STAT3-dependent manner. Silencing Hoxa10 abrogated the effects of G-CSF on promoting decidualization.Conclusion: G-CSF enhances decidualization of endometrial stromal cells via STAT3/HOXA10 axis activation. These findings suggest that optimal G-CSF delivery strategies could improve endometrial receptivity in RIF patients.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400279"},"PeriodicalIF":3.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post-Assembly Loading. 生物 PISA 中囊泡特性的表征和优化：从粒度分布到组装后装载。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-18 DOI: 10.1002/adbi.202400483

Andrea Belluati, Adrian Bloch, Kaloian Koynov, Mariana Müller Nieva, Mohadeseh Bagherabadi, Annette Andrieu-Brunsen, Harald Kolmar, Nico Bruns

引用次数: 0

Development of a pH-Responsive Antimicrobial and Potent Antioxidant Hydrogel for Accelerated Wound Healing: A Game Changer in Drug Delivery. 开发可加速伤口愈合的 pH 值响应型抗菌剂和强效抗氧化剂水凝胶：改变药物输送的游戏规则。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-14 DOI: 10.1002/adbi.202400358

Qixiang Gui, Neng Ding, Haimei Wu, Jinyue Liu, Yingnan Geng, Jie Zhu, Mingyue Gao, Antong Du, Bingbing Yue, Lie Zhu

{"title":"Development of a pH-Responsive Antimicrobial and Potent Antioxidant Hydrogel for Accelerated Wound Healing: A Game Changer in Drug Delivery.","authors":"Qixiang Gui, Neng Ding, Haimei Wu, Jinyue Liu, Yingnan Geng, Jie Zhu, Mingyue Gao, Antong Du, Bingbing Yue, Lie Zhu","doi":"10.1002/adbi.202400358","DOIUrl":"https://doi.org/10.1002/adbi.202400358","url":null,"abstract":"Stimuli-responsive hydrogels have the capability to alter their state in response to changes in physiological signals within their application environment, providing distinct benefits in drug delivery applications. Here, the acidic pH typically found in acutely infected wounds can be effectively managed by incorporating a pH-responsive Ag+ loaded system within the hydrogel, thereby ensuring efficient drug use and preventing potential toxicity from the sudden release of silver ions. The antimicrobial composite hydrogel HAMA/GelMA-CA/Ag+ provides some tissue adhesion and accelerates wound healing. GelMA-CA is synthesized by modifying gelatin methacryloyl (GelMA) with caffeic acid (CA), while hyaluronic acid methacryloyl (HAMA) is introduced to prepare a double network hydrogel. Silver nitrate is then introduced to make it pH-responsive through the formation of coordination between the polyphenolic structure of caffeic acid and the silver ions. The composite hydrogel exhibited excellent antioxidant properties and strong antimicrobial activity against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the composite hydrogel accelerated the promotion of wound healing in a rat model of S. aureus-infected wounds. In conclusion, the HAMA/GelMA-CA/Ag+ hydrogel is a promising bioactive material that can be used as a wound dressing to promote the healing of acutely infected wounds.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400358"},"PeriodicalIF":3.2,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevention of Intrauterine Adhesion with Platelet-Rich Plasma Double-Network Hydrogel. 用富血小板血浆双网水凝胶预防宫内粘连

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-14 DOI: 10.1002/adbi.202400336

Zhuomin Wang, Ying Gu, Yiyuan Qu, Xujia Huang, Tao Sun, Wei Wu, Qianyu Hu, Xiao Chen, Yu Li, Huafei Zhao, Yingying Hu, Bingbing Wu, Jian Xu

{"title":"Prevention of Intrauterine Adhesion with Platelet-Rich Plasma Double-Network Hydrogel.","authors":"Zhuomin Wang, Ying Gu, Yiyuan Qu, Xujia Huang, Tao Sun, Wei Wu, Qianyu Hu, Xiao Chen, Yu Li, Huafei Zhao, Yingying Hu, Bingbing Wu, Jian Xu","doi":"10.1002/adbi.202400336","DOIUrl":"https://doi.org/10.1002/adbi.202400336","url":null,"abstract":"Intrauterine adhesion (IUA) can negatively impact on pregnancy outcomes, leading to reduced pregnancy rates, secondary infertility, and an increased risk of pregnancy complications. Studies have shown that the application of platelet-rich plasma (PRP) in IUA patients is effective. However, the clinical readhesive rate of IUA after treatment is still high, especially in severe cases. Platelet-rich plasma double-network hydrogel (DN gel) is an engineered PRP double network hydrogel, which is a sodium alginate (SA) based PRP hydrogel with egg carton ion cross-linking and fibrin double network. The results of this study show that intrauterine injection of DN gel has a better effect on promoting endometrial regeneration and enhancing endometrial receptivity than PRP gel. The mechanism is analyzed through single-cell sequencing, which may be achieved by increasing the expression of neutrophils (Neut), natural killer cells (NK), and type I classical dendritic cells (cDC1) in the endometrium and inhibiting the infiltration of M2 macrophages. Overall, based on the good healing efficiency and good biocompatibility of DN gel, it is expected to become a method of treating IUA with better efficacy and faster clinical translation.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400336"},"PeriodicalIF":3.2,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Biodegradability and Cellular Compatibility of Mg-Zn-Nd Alloy for Vascular Stent Application. 评估血管支架应用中 Mg-Zn-Nd 合金的生物降解性和细胞兼容性。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1002/adbi.202400165

Yiqi Xing, Lili Tan, Zheng Ma, Tingzhun Zhu, Guobiao Liang

{"title":"Evaluating the Biodegradability and Cellular Compatibility of Mg-Zn-Nd Alloy for Vascular Stent Application.","authors":"Yiqi Xing, Lili Tan, Zheng Ma, Tingzhun Zhu, Guobiao Liang","doi":"10.1002/adbi.202400165","DOIUrl":"10.1002/adbi.202400165","url":null,"abstract":"The study is designed to evaluate the corrosion behavior, biocompatibility, and cytotoxicity of a novel magnesium alloy, Mg-2Zn-0.5Nd (ZN20), for potential use as biodegradable scaffolding in cerebrovascular stents. Magnesium alloy (AZ31) and ZN20 are co-cultured with Human Umbilical Vein Endothelial Cells (HUVEC) and human neuroblastoma cell (SH-SY5Y), respectively. The corrosion of AZ31 and ZN20 in different time periods is detected by electron microscope, the effects of AZ31 and ZN20 on the expression level of inflammatory factors are detected by ELISA, the PH value of cells in each group is detected, and the cell proliferation is detected by cck-8. Cell-related apoptosis protein, the expression of Platelet endothelial cell adhesion molecule-1 (CD31) and VE-cad is detected, and the pathological analysis of rat vascular tissue is carried out by HE experiment. In contrast to the AZ31 group, ZN20 exhibits mild, uniform corrosion and does not significantly deter HUVEC proliferation or increase inflammatory markers and In vivo testing reveals better endothelization with ZN20, as demonstrated by higher expression of endothelial markers CD31, and intact endothelial structure group. Western blotting shows favorable expression levels of apoptotic and anti-apoptotic markers in the ZN20 group. ZN20 alloy demonstrates enhanced corrosion resistance, favorable endothelial compatibility, and reduced cytotoxicity, endorsing its safe application in vascular stent use.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400165"},"PeriodicalIF":3.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing. 电针通过改善视网膜线粒体功能减缓实验性近视的发展：基于单细胞 RNA 测序的研究。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-01 Epub Date: 2024-10-15 DOI: 10.1002/adbi.202400269

Jiawen Hao, Yunxiao Xie, Huixia Wei, Zhaohui Yang, Ruixue Zhang, Zhongyu Ma, Miao Zhang, Xiaoshi Du, Xuewei Yin, Jinpeng Liu, Bo Bao, Hongsheng Bi, Dadong Guo

{"title":"Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing.","authors":"Jiawen Hao, Yunxiao Xie, Huixia Wei, Zhaohui Yang, Ruixue Zhang, Zhongyu Ma, Miao Zhang, Xiaoshi Du, Xuewei Yin, Jinpeng Liu, Bo Bao, Hongsheng Bi, Dadong Guo","doi":"10.1002/adbi.202400269","DOIUrl":"10.1002/adbi.202400269","url":null,"abstract":"This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is used to assess changes in individual cellular gene levels in the retina of LIM- and EA-treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA-seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain-related genes in Cones and Muller-glia cells-Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens-induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single-cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400269"},"PeriodicalIF":3.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qianggu Decoction Alleviated Osteoporosis by Promoting Osteogenesis of BMSCs through Mettl3-Mediated m⁶A Methylation. 羌活煎剂通过Mettl3介导的m6A甲基化促进BMSCs骨生成，从而缓解骨质疏松症

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-01 Epub Date: 2024-07-25 DOI: 10.1002/adbi.202400341

Yuchen Wang, Weizhong Yu, Yuan E, Lining Rui, Chuan Jia, Wenke Zhu

{"title":"Qianggu Decoction Alleviated Osteoporosis by Promoting Osteogenesis of BMSCs through Mettl3-Mediated m6A Methylation.","authors":"Yuchen Wang, Weizhong Yu, Yuan E, Lining Rui, Chuan Jia, Wenke Zhu","doi":"10.1002/adbi.202400341","DOIUrl":"10.1002/adbi.202400341","url":null,"abstract":"Osteoporosis development is linked to abnormal bone marrow mesenchymal stem cells (BMSCs) differentiation. N6-methyladenosine (m6A), a prevalent mRNA modification, is known to influence BMSCs' osteogenic capacity. Qianggu decoction (QGD), a traditional Chinese medicine for osteoporosis, has unknown effects on BMSCs differentiation. This study investigates QGD's impact on BMSCs and its potential to ameliorate osteoporosis through m6A regulation. Using Sprague-Dawley (SD) rats with ovariectomy-induced osteoporosis, it is evaluated QGD's antiosteoporotic effects through micro-CT, histology, Western blotting, and osteoblastogenesis markers. QGD is found to enhance bone tissue growth and upregulate osteogenic markers Runx2, OPN, and OCN. It also promoted BMSCs osteogenic differentiation, as shown by increased calcium nodules and ALP activity. QGD treatment significantly increased m6A RNA levels and Mettl3 expression in BMSCs. Silencing Mettl3 with siRNA negated QGD's osteogenic effects. Collectively, QGD may improve BMSCs differentiation and mitigate osteoporosis, potentially through Mettl3-mediated m6A modification.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400341"},"PeriodicalIF":3.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

o⁸G-miR-6513-5p/BCL2L13 Axis Regulates Mitophagy during Oxidative Stress in the Human Saphenous Vein Endothelial Cells. o8G-miR-6513-5p/BCL2L13 轴调控人隐静脉内皮细胞在氧化应激过程中的有丝分裂。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-01 Epub Date: 2024-09-22 DOI: 10.1002/adbi.202400218

Hao Jia, Le Kang, Ben Huang, Shuyang Lu, Zhiwen Ding, Zhenhang Chen, Chunsheng Wang, Jiangping Song, Yunzeng Zou, Yongxin Sun

引用次数: 0