Advanced biology最新文献_第10页

Toosendanin Induces Lung Squamous Cell Carcinoma Cell Apoptosis and Inhibits Tumor Progression via the BNIP3/AMPK Signaling Pathway Toosendanin 通过 BNIP3/AMPK 信号通路诱导肺鳞状细胞癌细胞凋亡并抑制肿瘤进展

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-21 DOI: 10.1002/adbi.202300610

Fabing Liu, Guangxue Wang, Liming Zhao, Guohan Chen, Lin Dong, Qinchuan Li, Dongyi Zhu

{"title":"Toosendanin Induces Lung Squamous Cell Carcinoma Cell Apoptosis and Inhibits Tumor Progression via the BNIP3/AMPK Signaling Pathway","authors":"Fabing Liu, Guangxue Wang, Liming Zhao, Guohan Chen, Lin Dong, Qinchuan Li, Dongyi Zhu","doi":"10.1002/adbi.202300610","DOIUrl":"10.1002/adbi.202300610","url":null,"abstract":"Lung squamous cell carcinoma (LUSC) is the second most common type of non-small cell lung cancer. Toosendanin can target critical cancer cell survival and proliferation. However, the function of toosendanin in LUSC is limited. Cancer cell proliferative capacity is detected using cell morphology, colony formation, and flow cytometry. The invasiveness of the cells is detected by a Transwell assay, western blotting, and RT-qPCR. Nude mice are injected with H226 (1×106) and received an intraperitoneal injection of toosendanin every 2 days for 21 days. RNA sequence transcriptome analysis is performed on toosendanin-treated cells to identify target genes and signaling pathways. With increasing concentrations of toosendanin, the rate of cell proliferation decreases and apoptotic cells increases. The number of migrated cells significantly reduces and epithelial-mesenchymal transition is reversed. Injection of toosendanin in nude mice leads to a reduction in tumor volume, weight, and the number of metastatic tumors. Furthermore, KEGG shows that genes related to the AMPK pathway are highly enriched. BNIP3 is the most differentially expressed gene, and its expression along with phosphorylated-AMPK significantly increases in toosendanin-treated cells. Toosendanin exerts anticancer effects, induces apoptosis in LUSC cells, and inhibits tumor progression via the BNIP3/AMPK signaling pathway.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Endurance and Resistance Exercise Mitigates Age-Associated Cardiac Dysfunction. 耐力和阻力运动相结合可减轻与年龄相关的心脏功能障碍

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-21 DOI: 10.1002/adbi.202400137

Xiaowei Han, Muhammad Ashraf, Hong Shi, Augustine T Nkembo, Srinivas M Tipparaju, Wanling Xuan

{"title":"Combined Endurance and Resistance Exercise Mitigates Age-Associated Cardiac Dysfunction.","authors":"Xiaowei Han, Muhammad Ashraf, Hong Shi, Augustine T Nkembo, Srinivas M Tipparaju, Wanling Xuan","doi":"10.1002/adbi.202400137","DOIUrl":"10.1002/adbi.202400137","url":null,"abstract":"Aging is associated with a decline in cardiac function. Exercise has been shown to effectively reduce the risks of cardiovascular diseases. Here whether a combination of endurance and resistance exercises can improve cardiac function in aged mice during late life is investigated. Through transcriptome analysis, several signaling pathways activated in the hearts of 22-month-old mice after combined exercise, including cardiac muscle contraction, mitophagy, and longevity regulation are identified. Combined exercise training mitigated age-associated pathological cardiac hypertrophy, reduced oxidative stress, cardiac senescence, and enhanced cardiac function. Upstream stimulatory factor 2 (Usf2) is upregulated in the aged mouse hearts with combined exercise compared to sedentary mice. In the human cardiomyocytes senescent model, overexpression of Usf2 led to anti-senescence effects, while knockdown of Usf2 exacerbated cellular senescence. The results suggest that a combination of endurance and resistance exercises, such as swimming and resistance running, can mitigate age-related pathological cardiac remodeling and cardiac dysfunction in late life. These cardioprotective effects are likely due to the activation of Usf2 and its anti-senescence effect. Therefore, Usf2 can potentially be a novel therapeutic target for mitigating age-related cardiac dysfunction.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Receptor in Synthetic Cells Performs Transmembrane Activation of Proteolysis. 合成细胞中的人工受体可执行跨膜激活蛋白水解。

IF 4.1 3区生物学

Advanced biology Pub Date : 2024-05-20 DOI: 10.1002/adbi.202400053

Ane Bretschneider Søgaard, Kaja Borup Løvschall, Mireia Casanovas Montasell, Clara Bakkegaard Cramer, Pere Monge Marcet, Andreas Bøtker Pedersen, Josefine Hammer Jakobsen, Alexander N Zelikin

{"title":"Artificial Receptor in Synthetic Cells Performs Transmembrane Activation of Proteolysis.","authors":"Ane Bretschneider Søgaard, Kaja Borup Løvschall, Mireia Casanovas Montasell, Clara Bakkegaard Cramer, Pere Monge Marcet, Andreas Bøtker Pedersen, Josefine Hammer Jakobsen, Alexander N Zelikin","doi":"10.1002/adbi.202400053","DOIUrl":"https://doi.org/10.1002/adbi.202400053","url":null,"abstract":"The design of artificial, synthetic cells is a fundamentally important and fast-developing field of science. Of the diverse attributes of cellular life, artificial transmembrane signaling across the biomolecular barriers remains a high challenge with only a few documented successes. Herein, the study achieves signaling across lipid bilayers and connects an exofacial enzymatic receptor activation to an intracellular biochemical catalytic response using an artificial receptor. The mechanism of signal transduction for the artificial receptor relies on the triggered decomposition of a self-immolative linker. Receptor activation ensues its head-to-tail decomposition and the release of a secondary messenger molecule into the internal volume of the synthetic cell. Transmembrane signaling is demonstrated in synthetic cells based on liposomes and mammalian cell-sized giant unilamellar vesicles and illustrates receptor performance in cell mimics with a diverse size and composition of the lipid bilayer. In giant unilamellar vesicles, transmembrane signaling connects exofacial receptor activation with intracellular activation of proteolysis. Taken together, the results of this study take a step toward engineering receptor-mediated, responsive behavior in synthetic cells.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphic Structure Determination of the Macrocyclic Drug Paritaprevir by MicroED (Adv. Biology 5/2024) 利用 MicroED 测定大环药物帕立普韦的多态结构（生物学进展 5/2024）

IF 4.1 3区生物学

Advanced biology Pub Date : 2024-05-13 DOI: 10.1002/adbi.202470051

Guanhong Bu, Emma Danelius, Lianne H.E. Wieske, Tamir Gonen

引用次数: 0

Masthead: (Adv. Biology 5/2024) 刊头：（高级生物学 5/2024）

IF 4.1 3区生物学

Advanced biology Pub Date : 2024-05-13 DOI: 10.1002/adbi.202470052

引用次数: 0

CellMag-CARWash: A High Throughput Droplet Microfluidic Device for Live Cell Isolation and Single Cell Applications CellMag-CARWash：用于活细胞分离和单细胞应用的高通量液滴微流控装置

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-13 DOI: 10.1002/adbi.202400066

Brittany T. Rupp, Claire D. Cook, Emma A. Purcell, Matei Pop, Abigail E. Radomski, Nicolas Mesyngier, Ryan C. Bailey, Sunitha Nagrath

{"title":"CellMag-CARWash: A High Throughput Droplet Microfluidic Device for Live Cell Isolation and Single Cell Applications","authors":"Brittany T. Rupp, Claire D. Cook, Emma A. Purcell, Matei Pop, Abigail E. Radomski, Nicolas Mesyngier, Ryan C. Bailey, Sunitha Nagrath","doi":"10.1002/adbi.202400066","DOIUrl":"10.1002/adbi.202400066","url":null,"abstract":"The recent push toward understanding an individual cell's behavior and identifying cellular heterogeneity has created an unmet need for technologies that can probe live cells at the single-cell level. Cells within a population are known to exhibit heterogeneous responses to environmental cues. These differences can lead to varied cellular states, behavior, and responses to therapeutics. Techniques are needed that are not only capable of processing and analyzing cellular populations at the single cell level, but also have the ability to isolate specific cell populations from a complex sample at high throughputs. The new CellMag-Coalesce-Attract-Resegment Wash (CellMag-CARWash) system combines positive magnetic selection with droplet microfluidic devices to isolate cells of interest from a mixture with >93% purity and incorporate treatments within individual droplets to observe single cell biological responses. This workflow is shown to be capable of probing the single cell extracellular vesicle (EV) secretion of MCF7 GFP cells. This article reports the first measurement of β-Estradiol's effect on EV secretion from MCF7 cells at the single cell level. Single cell processing revealed that MCF7 GFP cells possess a heterogeneous response to β-Estradiol stimulation with a 1.8-fold increase relative to the control.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast Cancer Immune Landscape: Interplay Between Systemic and Local Immunity 乳腺癌免疫格局：全身免疫与局部免疫的相互作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-10 DOI: 10.1002/adbi.202400140

Tatiana Gerashchenko, Anastasia Frolova, Marina Patysheva, Anton Fedorov, Marina Stakheyeva, Evgeny Denisov, Nadezda Cherdyntseva

{"title":"Breast Cancer Immune Landscape: Interplay Between Systemic and Local Immunity","authors":"Tatiana Gerashchenko, Anastasia Frolova, Marina Patysheva, Anton Fedorov, Marina Stakheyeva, Evgeny Denisov, Nadezda Cherdyntseva","doi":"10.1002/adbi.202400140","DOIUrl":"10.1002/adbi.202400140","url":null,"abstract":"Breast cancer (BC) is one of the most common malignancies in women worldwide. Numerous studies in immuno-oncology and successful trials of immunotherapy have demonstrated the causal role of the immune system in cancer pathogenesis. The interaction between the tumor and the immune system is known to have a dual nature. Despite cytotoxic lymphocyte activity against transformed cells, a tumor can escape immune surveillance and leverage chronic inflammation to maintain its own development. Research on antitumor immunity primarily focuses on the role of the tumor microenvironment, whereas the systemic immune response beyond the tumor site is described less thoroughly. Here, a comprehensive review of the formation of the immune profile in breast cancer patients is offered. The interplay between systemic and local immune reactions as self-sustaining mechanism of tumor progression is described and the functional activity of the main cell populations related to innate and adaptive immunity is discussed. Additionally, the interaction between different functional levels of the immune system and their contribution to the development of the pro- or anti-tumor immune response in BC is highlighted. The presented data can potentially inform the development of new immunotherapy strategies in the treatment of patients with BC.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obeticholic Acid Inhibit Mitochondria Dysfunction Via Regulating ERK1/2-DRP Pathway to Exert Protective Effect on Lipopolysaccharide-Induced Myocardial Injury 奥贝胆酸通过调节ERK1/2-DRP通路抑制线粒体功能障碍，从而对脂多糖诱发的心肌损伤发挥保护作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-10 DOI: 10.1002/adbi.202300576

Huijie Miao, Xiaomeng Tang, Yun Cui, Jingyi Shi, Xi Xiong, Chunxia Wang, Yucai Zhang

{"title":"Obeticholic Acid Inhibit Mitochondria Dysfunction Via Regulating ERK1/2-DRP Pathway to Exert Protective Effect on Lipopolysaccharide-Induced Myocardial Injury","authors":"Huijie Miao, Xiaomeng Tang, Yun Cui, Jingyi Shi, Xi Xiong, Chunxia Wang, Yucai Zhang","doi":"10.1002/adbi.202300576","DOIUrl":"10.1002/adbi.202300576","url":null,"abstract":"Farnesoid X receptor (FXR) plays critical regulatory roles in cardiovascular physiology/pathology. However, the role of FXR agonist obeticholic acid (OCA) in sepsis-associated myocardial injury and underlying mechanisms remain unclear. C57BL/6J mice are treated with OCA before lipopolysaccharide (LPS) administration. The histopathology of the heart and assessment of FXR expression and mitochondria function are performed. To explore the underlying mechanisms, H9c2 cells, and primary cardiomyocytes are pre-treated with OCA before LPS treatment, and extracellular signal-regulated protein kinase (ERK) inhibitor PD98059 is used. LPS-induced myocardial injury in mice is significantly improved by OCA pretreatment. Mechanistically, OCA pretreatment decreased reactive oxygen species (ROS) levels and blocked the loss of mitochondrial membrane potential (ΔΨm) in cardiomyocytes. The expression of glutathione peroxidase 1 (GPX1), superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and nuclear factor erythroid 2-related factor 2 (NRF-2) increased in the case of OCA pretreatment. In addition, OCA improved mitochondria respiratory chain with increasing Complex I expression and decreasing cytochrome C (Cyt-C) diffusion. Moreover, OCA pretreatment inhibited LPS-induced mitochondria dysfunction via suppressing ERK1/2-DRP signaling pathway. FXR agonist OCA inhibits LPS-induced mitochondria dysfunction via suppressing ERK1/2-DRP signaling pathway to protect mice against LPS-induced myocardial injury.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202300576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding Organelle Interactions: Unveiling Molecular Mechanisms and Disease Therapies 解码细胞器相互作用：揭示分子机制和疾病疗法。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-08 DOI: 10.1002/adbi.202300288

Ruixue Liu, Weilong Hong, Dongyao Hou, He Huang, Chenyang Duan

引用次数: 0

The ICL1 and MLS1 Genes, Integral to the Glyoxylate Cycle, are Essential and Specific for Caloric Restriction-Mediated Extension of Lifespan in Budding Yeast ICL1和MLS1基因是乙醛酸循环的组成部分，它们对于热量限制介导的芽殖酵母寿命延长是必要的和特异的。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-05-08 DOI: 10.1002/adbi.202400083

Young-Yon Kwon, Han-Jun Lee, Myung-Jin Lee, Young-Sam Lee, Cheol-Koo Lee

{"title":"The ICL1 and MLS1 Genes, Integral to the Glyoxylate Cycle, are Essential and Specific for Caloric Restriction-Mediated Extension of Lifespan in Budding Yeast","authors":"Young-Yon Kwon, Han-Jun Lee, Myung-Jin Lee, Young-Sam Lee, Cheol-Koo Lee","doi":"10.1002/adbi.202400083","DOIUrl":"10.1002/adbi.202400083","url":null,"abstract":"The regulation of complex energy metabolism is intricately linked to cellular energy demands. Caloric restriction (CR) plays a pivotal role in modulating the expression of genes associated with key metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and the glyoxylate cycle. In this study, the chronological lifespan (CLS) of 35 viable single-gene deletion mutants under both non-restricted and CR conditions, focusing on genes related to these metabolic pathways is evaluated. CR is found to increase CLS predominantly in mutants associated with the glycolysis and TCA cycle. However, this beneficial effect of CR is not observed in mutants of the glyoxylate cycle, particularly those lacking genes for critical enzymes like isocitrate lyase 1 (icl1Δ) and malate synthase 1 (mls1Δ). This analysis revealed an increase in isocitrate lyase activity, a key enzyme of the glyoxylate cycle, under CR, unlike the activity of isocitrate dehydrogenase, which remains unchanged and is specific to the TCA cycle. Interestingly, rapamycin, a compound known for extending lifespan, does not increase the activity of the glyoxylate cycle enzyme. This suggests that CR affects lifespan through a distinct metabolic mechanism.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0