Advanced biology最新文献_第10页

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-19

引用次数: 0

Bioelectronic Technology for Nutritional Research—a Novel In Vitro Platform for a Better Understanding of Human Gut Barrier Absorption 用于营养研究的生物电子技术——更好地了解人体肠道屏障吸收的新型体外平台。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500409

Verena Stoeger, Magdalena Strauss, Kumar Thurimella, Shani Elias-Kirma, Izabella Niewczas, Etkin Parlar, Erika Schaudy, Chrysanthi-Maria Moysidou, Stephen Voong, Jory Lietard, Jonathan Clark, Christopher Gerner, Róisín M. Owens

{"title":"Bioelectronic Technology for Nutritional Research—a Novel In Vitro Platform for a Better Understanding of Human Gut Barrier Absorption","authors":"Verena Stoeger, Magdalena Strauss, Kumar Thurimella, Shani Elias-Kirma, Izabella Niewczas, Etkin Parlar, Erika Schaudy, Chrysanthi-Maria Moysidou, Stephen Voong, Jory Lietard, Jonathan Clark, Christopher Gerner, Róisín M. Owens","doi":"10.1002/adbi.202500409","DOIUrl":"10.1002/adbi.202500409","url":null,"abstract":"<p>The epithelial gut barrier and gut microbiota significantly contribute to human health by controlling molecule absorption, a regulated transport that dictates bioavailability. Effective public health strategies, like dietary reference values, require a complete understanding of nutrient absorption. However, the lack of internationally harmonized nutritional recommendations indicates that gut barrier mechanisms are not fully unraveled. The conventional in vitro model Caco-2/HT29-MTX cultured on cell culture inserts, established for drug development, is limited in representing complex human gut physiology. The new bioelectronic e-transmembrane platform leverages technological and biological advances to generate more meaningful in vitro predictions. The soft electroactive Poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) scaffold enables direct cell-electrode coupling for more sensitive barrier impedance measurements, especially required for testing commonly low physiological nutrient concentrations. Promoted epithelial-fibroblast interactions result in modulated protein signal transduction and expression of genes regulating gut barrier integrity. Overall, the e-transmembrane gut barrier more closely mimicked physiological effects for humans as demonstrated using the dietary compound butyrate.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Therapeutic Targets and Signaling Networks in Ischemia–Reperfusion Injury 缺血再灌注损伤的新治疗靶点和信号网络。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500673

Shreeya Bhujbal, Lokesh Kumar Bhatt

{"title":"Emerging Therapeutic Targets and Signaling Networks in Ischemia–Reperfusion Injury","authors":"Shreeya Bhujbal, Lokesh Kumar Bhatt","doi":"10.1002/adbi.202500673","DOIUrl":"10.1002/adbi.202500673","url":null,"abstract":"<div>\u0000 \u0000 <p>Ischemia–reperfusion injury remains a major challenge in modern regenerative medicine due to its complex mechanisms, lack of effective therapies, and persistent constrains to translating new interventions from bench to bedside. Currently, there are no FDA-approved drugs that directly target ischemia–reperfusion injury, highlighting a substantial therapeutic gap. While restoring blood flow is vital for salvaging ischemic tissue, the reperfusion process paradoxically triggers additional cellular damage. The clinical significance and complexity of ischemia–reperfusion injury underscore an urgent need for mechanistically targeted therapeutic approaches. Recent research has identified pivotal molecular targets—PHLDA1, SIRT6, PKM2, and ubiquitin-specific proteases (USPs)—that play key roles in modulating cellular responses such as oxidative stress, inflammation, metabolism, apoptosis, autophagy, ferroptosis, and blood-brain barrier dysfunction during ischemia reperfusion injury. Advances in understanding these mechanisms offer promising strategies for developing novel interventions to mitigate tissue damage and improve patient outcomes. This review critically examines these molecular targets, detailing recent advances and outlining future directions in ischemia reperfusion injury research.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18

引用次数: 0

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18

引用次数: 0

Notoginsenoside R1 as a Protector Against Gentamicin Ototoxicity: Targeting p62-NRF2-GPX4 Ferroptosis Axis 三七皂苷R1作为抗Gentamicin耳毒性的保护剂：靶向p62-NRF2-GPX4 Ferroptosis轴

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500580

Xiaorui Chen, Ruoyu Jiang, Yunfeng Chu, Yan Sun, Zijiang Yuan, Yuhan Qian, Xiaolan Cheng, Hongjun Dong

{"title":"Notoginsenoside R1 as a Protector Against Gentamicin Ototoxicity: Targeting p62-NRF2-GPX4 Ferroptosis Axis","authors":"Xiaorui Chen, Ruoyu Jiang, Yunfeng Chu, Yan Sun, Zijiang Yuan, Yuhan Qian, Xiaolan Cheng, Hongjun Dong","doi":"10.1002/adbi.202500580","DOIUrl":"10.1002/adbi.202500580","url":null,"abstract":"<div>\u0000 \u0000 <p>Sudden sensorineural hearing loss (SSNHL) is a common auditory disorder, with oxidative stress and ferroptosis implicated in its pathogenesis. Notoginsenoside R1, a major component of Panax notoginseng, exhibits antioxidant and cytoprotective effects, but its role in ferroptosis regulation remains unclear. In this study, a mouse model of SSNHL and an oxygen-glucose deprivation/reoxygenation (OGD/R)-induced Ear Institute-Organ of Corti 1 (HEI-OC1) cell model were used to evaluate the effects of Notoginsenoside R1. Notoginsenoside R1 significantly reduced auditory brainstem response (ABR) thresholds, reduced cochlear hair cell apoptosis, and alleviated oxidative damage. It inhibited ferroptosis by decreasing Fe<sup>2</sup><sup>+</sup>, ROS, MDA, and LPO levels, while upregulating SOD, GSH, GCL, and GPX4. Mechanistically, nuclear factor erythroid 2-related factor 2 (NRF2) was identified as a key responsive target through target prediction and qPCR validation, while molecular docking and microscale thermophoresis (MST) assays demonstrated a direct interaction between Notoginsenoside R1 and SQSTM1 (p62). Besides, Notoginsenoside R1 enhanced p62 expression and promoted NRF2 nuclear translocation, restoring expression of GCLC, GCLM, and GPX4. Both p62 knockout and siRNA-mediated knockdown progressively attenuated these protective effects. These findings suggest that Notoginsenoside R1 protects against SSNHL by inhibiting ferroptosis and oxidative stress via activation of the p62-NRF2-GCL-GPX4 axis.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sub-Neuronal Network Profiling of Extracellular Vesicle Release Using a Compartmentalized Neurofluidic Platform 使用区隔化神经流体平台的细胞外囊泡释放的亚神经元网络分析。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500381

Zeynep Malkoc, Esther Stopps, Prince M. K. Asamoah, Stephanie E. McCalla, Anja Kunze

{"title":"Sub-Neuronal Network Profiling of Extracellular Vesicle Release Using a Compartmentalized Neurofluidic Platform","authors":"Zeynep Malkoc, Esther Stopps, Prince M. K. Asamoah, Stephanie E. McCalla, Anja Kunze","doi":"10.1002/adbi.202500381","DOIUrl":"10.1002/adbi.202500381","url":null,"abstract":"<p>Extracellular vesicles (EVs) are membrane-bound vesicles that are secreted by a wide range of organisms and cells, carrying cell-specific receptors and molecular cargo such as proteins and nucleic acids. EVs have emerged as promising biomarkers for cancer and neurodegenerative disorders like Alzheimer's Disease (AD). Traditional methods for isolating neuron-derived EVs from bodily fluids or conditioned media are based on bulk analysis methods, such as ultracentrifugation, isolation reagents, and immunoaffinity-based techniques, and lack spatial resolution to capture localized secretion dynamics. Here, our neurofluidic platform compartmentalizes neuronal networks and enables spatially resolved analysis of EV profiling before subsequent traditional isolation and content screening. This intermediate resolution provides critical insights into localized sub-neuronal EV secretion dynamics in cortical, hippocampal, and brainstem neurons. Using our platform, the influence of growth environment, cell maturation time, and exogenous stressors such as shear and biochemical stress can be unraveled. Biochemical stress is induced through okadaic acid (OA), a PP1A/PP2A inhibitor, which leads to hyperphosphorylation of proteins. In parallel, microRNA expression profiles are shown after OA treatment in primary neuron cultures, indicating an additional transcriptional response. These findings reveal regional differences in EV secretion dynamics associated with neuronal development and external stressors, including shear forces and PP1A/PP2A inhibition.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12914630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Common Immune Responses Differences from Transcriptomic Profiling in Cardiomyocytes upon Coxsackievirus B5 and Echovirus 6 Infection 柯萨奇病毒B5和埃可病毒6感染心肌细胞转录组学分析的共同免疫反应差异

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500281

Yi Xu, Xianwu Lan, Jianwei Chen, Jieyu You, Hiu Tung Ko, Chuqun Fang, Shanshan Cui, Muhammad Adnan Shereen, Zhen Luo, Shaorong Wu

{"title":"The Common Immune Responses Differences from Transcriptomic Profiling in Cardiomyocytes upon Coxsackievirus B5 and Echovirus 6 Infection","authors":"Yi Xu, Xianwu Lan, Jianwei Chen, Jieyu You, Hiu Tung Ko, Chuqun Fang, Shanshan Cui, Muhammad Adnan Shereen, Zhen Luo, Shaorong Wu","doi":"10.1002/adbi.202500281","DOIUrl":"10.1002/adbi.202500281","url":null,"abstract":"<div>\u0000 \u0000 <p>Viral myocarditis (VMC) is a myocardial injury syndrome caused by enterovirus infections, including Coxsackievirus B5 (CVB5) and Echovirus 6 (ECHO6). However, the exact pathogenesis of VMC by enteroviruses remains unclear. Here, the host immune response differences in transcriptomics in human cardiomyocyte AC16 cells upon CVB5 and ECHO6 infections were explored. CVB5 and ECHO6 effectively infected AC16 cells, showing significant viral replication and cytopathic effects at 48 h post-infection. Transcriptomic analysis indicated that both CVB5 and ECHO6 infection induced a series of immune- and inflammation- related genes, including IL6, CCL3, and IFNL1, which were validated by qPCR. Additionally, Ribavirin demonstrated a certain inhibitory effect on the viral replication of both CVB5 and ECHO6 at a concentration of 50 µ<span>m</span>. This study established a systematic comparison of the common transcriptomic differences and immune response characteristics of both CVB5 and ECHO6 infection in human cardiomyocytes. The marked upregulation of immune-related genes suggests that innate immunity and the inflammatory response play critical roles in cardiomyocytes defense against enterovirus infection. Ribavirin showed notable inhibitory activity against both CVB5 and ECHO6. The study sheds light on new insights into the foundations for the pathogenesis of enterovirus-associated VMC and the development of promising therapeutic approaches against VMC.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18

引用次数: 0

Suppression of LncRNA Dlx6os1 Alleviated High Glucose-Induced Oxidative Stress Mediated by Parkin in Cardiomyocytes LncRNA Dlx6os1抑制可缓解Parkin介导的高糖诱导的心肌细胞氧化应激。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500692

Jiayu Diao, Qiling Gou, Linting Zhang, Fengchao Wu, Lei Liang

{"title":"Suppression of LncRNA Dlx6os1 Alleviated High Glucose-Induced Oxidative Stress Mediated by Parkin in Cardiomyocytes","authors":"Jiayu Diao, Qiling Gou, Linting Zhang, Fengchao Wu, Lei Liang","doi":"10.1002/adbi.202500692","DOIUrl":"10.1002/adbi.202500692","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the effect of lncDlx6os1 on high glucose (HG)-induced oxidative stress and the involvement of the parkin pathway. Methods: Short hairpin RNA of lncDlx6-os1 (sh-lncDlx6os1) and small interfering RNA of Parkin (si-Parkin) were used to inhibit the expression of lncDlx6-os1 and Parkin in cardiomyocytes, respectively. qRT-PCR, western blotting, and flow cytometry were performed to investigate the underlying mechanism. Results: sh-lncDlx6os1 inhibited HG-induced reactive oxygen species (ROS) generation and increased the expression of HO-1 and NRF2 in HG-treated cardiomyocytes. sh-lncDlx6os1 significantly suppressed the expression of the apoptotic molecule caspase3, and increased the expression of the anti-apoptotic molecule Bcl-2, thereby inhibiting HG-induced apoptosis. Meanwhile, sh-lncDlx6os1 increased the expression of Sirt1, PINK1, Parkin, and LC3II/LC3I in HG-treated cardiomyocytes. Suppression of Parkin by si-Parkin weakened the regulatory effect of sh-lncDlx6os1 on apoptosis and caspase3 and Bcl-2 expression. si-Parkin also attenuated the inhibitory effect of sh-lncDlx6os1 on ROS generation and increased HO-1 and NRF2 expressions in HG-treated cardiomyocytes. Conclusion: Our study revealed that the suppression of lncDlx6os1 significantly alleviated HG-induced oxidative stress, which was partially mediated by parkin in cardiomyocytes.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0