Advanced biology最新文献_第9页

Accurate Identification of Cancer Cells in Complex Pre-Clinical Models Using a Deep-Learning Neural Network: A Transfection-Free Approach (Adv. Biology 11/2024) 利用深度学习神经网络准确识别复杂临床前模型中的癌细胞：无转染方法（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470112

Marilisa Cortesi, Dongli Liu, Elyse Powell, Ellen Barlow, Kristina Warton, Caroline E. Ford

引用次数: 0

Masthead: (Adv. Biology 11/2024) 刊头：（Adv. Biology 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470113

引用次数: 0

Predicting Clinical Outcomes of SARS-CoV-2 Drug Efficacy with a High-Throughput Human Airway Microphysiological System (Adv. Biology 11/2024) 利用高通量人体气道微生理系统预测SARS-CoV-2药物疗效的临床结果（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470111

Landys Lopez Quezada, Felix Mba Medie, Rebeccah J. Luu, Robert B. Gaibler, Elizabeth P. Gabriel, Logan D. Rubio, Thomas J. Mulhern, Elizabeth E. Marr, Jeffrey T. Borenstein, Christine R. Fisher, Ashley L. Gard

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Evaluating the Biodegradability and Cellular Compatibility of Mg–Zn–Nd Alloy for Vascular Stent Application 评估血管支架应用中 Mg-Zn-Nd 合金的生物降解性和细胞兼容性。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-11 DOI: 10.1002/adbi.202400165

Yiqi Xing, Lili Tan, Zheng Ma, Tingzhun Zhu, Guobiao Liang

{"title":"Evaluating the Biodegradability and Cellular Compatibility of Mg–Zn–Nd Alloy for Vascular Stent Application","authors":"Yiqi Xing, Lili Tan, Zheng Ma, Tingzhun Zhu, Guobiao Liang","doi":"10.1002/adbi.202400165","DOIUrl":"10.1002/adbi.202400165","url":null,"abstract":"The study is designed to evaluate the corrosion behavior, biocompatibility, and cytotoxicity of a novel magnesium alloy, Mg-2Zn-0.5Nd (ZN20), for potential use as biodegradable scaffolding in cerebrovascular stents. Magnesium alloy (AZ31) and ZN20 are co-cultured with Human Umbilical Vein Endothelial Cells (HUVEC) and human neuroblastoma cell (SH-SY5Y), respectively. The corrosion of AZ31 and ZN20 in different time periods is detected by electron microscope, the effects of AZ31 and ZN20 on the expression level of inflammatory factors are detected by ELISA, the PH value of cells in each group is detected, and the cell proliferation is detected by cck-8. Cell-related apoptosis protein, the expression of Platelet endothelial cell adhesion molecule-1 (CD31) and VE-cad is detected, and the pathological analysis of rat vascular tissue is carried out by HE experiment. In contrast to the AZ31 group, ZN20 exhibits mild, uniform corrosion and does not significantly deter HUVEC proliferation or increase inflammatory markers and In vivo testing reveals better endothelization with ZN20, as demonstrated by higher expression of endothelial markers CD31, and intact endothelial structure group. Western blotting shows favorable expression levels of apoptotic and anti-apoptotic markers in the ZN20 group. ZN20 alloy demonstrates enhanced corrosion resistance, favorable endothelial compatibility, and reduced cytotoxicity, endorsing its safe application in vascular stent use.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"8 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging 通过数字全息显微镜和定量相位成像对黑色素细胞和 SK-MEL-28 黑色素瘤细胞进行形态学和光学分析。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-11 DOI: 10.1002/adbi.202400346

Ayah A. Farhat, Yazan A. Almahdi, Fatima Z. Alshuhani, Besa Xhabija

{"title":"Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging","authors":"Ayah A. Farhat, Yazan A. Almahdi, Fatima Z. Alshuhani, Besa Xhabija","doi":"10.1002/adbi.202400346","DOIUrl":"10.1002/adbi.202400346","url":null,"abstract":"Melanoma, which originates from pigment-producing melanocytes, is an aggressive and deadly skin cancer. Despite extensive research, its mechanisms of progression and metastasis remain unclear. This study uses quantitative phase imaging via digital holographic microscopy, Principal Component Analysis (PCA), and t-distributed Stochastic Neighbor Embedding (t-SNE) to identify the morphological, optical, and behavioral differences between normal melanocytes and SK-MEL-28 melanoma cells. Our findings reveal significant differences in cell shape, size, and internal organization, with SK-MEL-28 cells displaying greater size variability, more polygonal shapes, and higher optical thickness. Phase shift parameters and surface roughness analyses underscore melanoma cells' uniform and predictable textures. Violin plots highlight the dynamic and varied migration of SK-MEL-28 cells, contrasting with the localized movement of melanocytes. Hierarchical clustering of correlation matrices provides further insights into complex morphological and optical relationships. Integrating label-free imaging with robust analytical methods enhances understanding of melanoma's aggressive behavior, supporting targeted therapies and highlighting potential biomarkers for precise melanoma diagnostics and treatment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced BMP Signaling Alters Human β-Cell Identity and Function 增强的BMP信号改变了人类β细胞的特性和功能

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-05 DOI: 10.1002/adbi.202400470

Esmée Dekker, Javier Triñanes, Amadeo Muñoz Garcia, Natascha de Graaf, Eelco de Koning, Françoise Carlotti

{"title":"Enhanced BMP Signaling Alters Human β-Cell Identity and Function","authors":"Esmée Dekker, Javier Triñanes, Amadeo Muñoz Garcia, Natascha de Graaf, Eelco de Koning, Françoise Carlotti","doi":"10.1002/adbi.202400470","DOIUrl":"10.1002/adbi.202400470","url":null,"abstract":"Inflammation contributes to the pathophysiology of diabetes. Identifying signaling pathways involved in pancreatic β-cell failure and identity loss can give insight into novel potential treatment strategies to prevent the loss of functional β-cell mass in diabetes. It is reported earlier that the immunosuppressive drug tacrolimus has a detrimental effect on human β-cell identity and function by activating bone morphogenetic protein (BMP) signaling. Here it is hypothesized that enhanced BMP signaling plays a role in inflammation-induced β-cell failure. Single-cell transcriptomics analyses of primary human islets reveal that IL-1β+IFNγ and IFNα treatment activated BMP signaling in β-cells. These findings are validated by qPCR. Furthermore, enhanced BMP signaling with recombinant BMP2 or 4 triggers a reduced expression of key β-cell maturity genes, associated with increased ER stress, and impaired β-cell function. Altogether, these results indicate that inflammation-activated BMP signaling is detrimental to pancreatic β-cells and that BMP-signaling can be a target to preserve β-cell identity and function in a pro-inflammatory environment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xiao-Ban-Xia Decoction Alleviates Chemotherapy-Induced Nausea and Vomiting by Inhibiting Ferroptosis via Activation of The Nrf2/SLC7A11/GPX4 Pathway 小半夏煎剂通过激活Nrf2/SLC7A11/GPX4通路抑制铁变态反应，缓解化疗引起的恶心和呕吐

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-05 DOI: 10.1002/adbi.202400323

Wan Liang, Yuke Ren, Yusu Wang, Weijian Chen, Ziyao Mo, Chenglu Yang, Ke Nie

{"title":"Xiao-Ban-Xia Decoction Alleviates Chemotherapy-Induced Nausea and Vomiting by Inhibiting Ferroptosis via Activation of The Nrf2/SLC7A11/GPX4 Pathway","authors":"Wan Liang, Yuke Ren, Yusu Wang, Weijian Chen, Ziyao Mo, Chenglu Yang, Ke Nie","doi":"10.1002/adbi.202400323","DOIUrl":"10.1002/adbi.202400323","url":null,"abstract":"Chemotherapy-induced nausea and vomiting (CINV) represents the common gastrointestinal side effect for cancer patients. Xiao-Ban-Xia decoction (XBXD), a classical anti-emetic traditional Chinese medicine formula, is frequently used for the clinical treatment of CINV. This study used a cisplatin-induced rat pica model to explore whether the anti-emetic mechanism of XBXD in treating CINV is related to ferroptosis. The inflammatory damage of the gastrointestinal tract is evaluated by HE staining and ELISA. The degree of ferroptosis are validated by the iron deposition, the levels of ROS, MDA, and GSH, and the ultrastructure of mitochondria in the gastric antrum and ileum. The potential ferroptosis-related targets of XBXD against CINV are screened by network pharmacology and further assessed by Western blot. XBXD significantly decreased the kaolin consumption in rats, and improved the inflammatory pathological damage, with decreased levels of HMGB1, IL-1β, and TNF-α. Furthermore, XBXD significantly suppressed ferroptosis, as indicated by the improvement of iron deposition, mitochondrial abnormalities, and oxidative stress. The network pharmacology and Western blot results indicated that XBXD activated the Nrf2/SLC7A11/GPX4 signaling pathway. This study proved that XBXD activates the Nrf2/SLC7A11/GPX4 signaling pathway, thereby inhibiting ferroptosis, which represents a critical anti-emetic mechanism of XBXD in combatting CINV.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"8 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-12p70 Induces Neuroprotection via the PI3K-AKT-BCL2 Axis to Mediate the Therapeutic Effect of Electroacupuncture on Postoperative Cognitive Dysfunction IL-12p70通过PI3K-AKT-BCL2轴诱导神经保护，介导电针对术后认知功能障碍的治疗效果

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-30 DOI: 10.1002/adbi.202400172

Tingting Huang, Jie Hong, Jia Ling, Lin Zhu, Wei Zhao, Xinlu Zhang, Xinze Yan, Chen Hu, Ruijie Zhang, Chen Gao, Shengzhao Zhang, Chen Chen, Runhuai Yang, Weiwei Wu, Chunhui Wang, Qian Gao

{"title":"IL-12p70 Induces Neuroprotection via the PI3K-AKT-BCL2 Axis to Mediate the Therapeutic Effect of Electroacupuncture on Postoperative Cognitive Dysfunction","authors":"Tingting Huang, Jie Hong, Jia Ling, Lin Zhu, Wei Zhao, Xinlu Zhang, Xinze Yan, Chen Hu, Ruijie Zhang, Chen Gao, Shengzhao Zhang, Chen Chen, Runhuai Yang, Weiwei Wu, Chunhui Wang, Qian Gao","doi":"10.1002/adbi.202400172","DOIUrl":"10.1002/adbi.202400172","url":null,"abstract":"Postoperative cognitive dysfunction (POCD), a postsurgical decline in cognitive function, primarily affects older adults and worsens their prognosis. Although elevated interleukin-12p70 (IL-12p70) is closely correlated with slower cognitive decline in older adults, its role in POCD remains unclear. Here, IL-12p70 is identified as a significant mediator of therapeutic effect of electroacupuncture (EA) on POCD. EA at acupoints ST36, GV20, and GV24 significantly enhanced cognitive behaviors of POCD mice. IL-12p70, downregulated in POCD mice but rescued by EA treatment, is the cytokine closely associated with EA's therapeutic effect. Clinically, IL-12p70 is downregulated in older adults’ serum post-surgery. Furthermore, IL-12p70 exerts a potent neuroprotective effect in both neuronal cell lines and primary hippocampal neurons. The PI3K-AKT-BCL2 axis enriched by in silico analysis is validated as the signaling mechanism underlying IL-12p70-induced neuroprotection. In vivo, beneficial effects of EA treatment on the activation of PI3K-AKT-BCL2 axis and POCD are reproduced by IL-12p70 administration but attenuated by IL-12p70 knockdown. The findings reveal a novel mechanism underlying the therapeutic effect of EA on POCD, demonstrating that IL-12p70 exerts a neuroprotective effect by activating PI3K-AKT-BCL2 axis in hippocampal neurons. The newly-discovered function and mechanism of IL-12p70 highlight its potential in treating cognitive disorders.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Impact of Senescent Cell-Derived Extracellular Vesicles on Innate Immune Cell Function 衰老细胞衍生的细胞外囊泡对先天性免疫细胞功能的影响

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-23 DOI: 10.1002/adbi.202400265

Yung-Yi Chen, Jack Sullivan, Shaun Hanley, Joshua Price, Mohammad A. Tariq, Luke C. McIlvenna, Martin Whitham, Archana Sharma-Oates, Paul Harrison, Janet M. Lord, Jon Hazeldine

{"title":"Impact of Senescent Cell-Derived Extracellular Vesicles on Innate Immune Cell Function","authors":"Yung-Yi Chen, Jack Sullivan, Shaun Hanley, Joshua Price, Mohammad A. Tariq, Luke C. McIlvenna, Martin Whitham, Archana Sharma-Oates, Paul Harrison, Janet M. Lord, Jon Hazeldine","doi":"10.1002/adbi.202400265","DOIUrl":"10.1002/adbi.202400265","url":null,"abstract":"Extracellular vesicles (EVs) are components of the senescence-associated secretory phenotype (SASP) that influence cellular functions via their cargo. Here, the interaction between EVs derived from senescent (SEVs) and non-senescent (N-SEVs) fibroblasts and the immune system is investigated. Via endocytosis, SEVs are phagocytosed by monocytes, neutrophils, and B cells. Studies with the monocytic THP-1 cell line find that pretreatment with SEVs results in a 32% (p < 0.0001) and 66% (p < 0.0001) increase in lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-α) production when compared to vehicle control or N-SEVs respectively. Interestingly, relative to vehicle control, THP-1 cells exposed to N-SEVs exhibit a 20% decrease in TNF-α secretion (p < 0.05). RNA sequencing reveals significant differences in gene expression in THP-1 cells treated with SEVs or N-SEVs, with vesicle-mediated transport and cell cycle regulation pathways featuring predominantly with N-SEV treatment, while pathways relating to SLITS/ROBO signaling, cell metabolism, and cell cycle regulation are enriched in THP-1 cells treated with SEVs. Proteomic analysis also reveals significant differences between SEV and N-SEV cargo. These results demonstrate that phagocytes and B cells uptake SEVs and drive monocytes toward a more proinflammatory phenotype upon LPS stimulation. SEVs may therefore contribute to the more proinflammatory immune response seen with aging.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"8 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Update on Theoretical and Metrological Aspects of the Surface Hydrophobicity of Virus and Virus-Like Particles. 病毒和类病毒颗粒表面疏水性的理论和计量方面的最新情况。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-22 DOI: 10.1002/adbi.202400221

Guillaume Sautrey

{"title":"An Update on Theoretical and Metrological Aspects of the Surface Hydrophobicity of Virus and Virus-Like Particles.","authors":"Guillaume Sautrey","doi":"10.1002/adbi.202400221","DOIUrl":"https://doi.org/10.1002/adbi.202400221","url":null,"abstract":"Viruses are biological entities embodied in protein-based nanoparticles devoid of metabolic activity. Hence, the colloidal, interfacial, and chemical reactivity of virus particles (VPs) profoundly affects the fate of natural and artificial viruses in biotic or abiotic aqueous systems. These rely on the physical chemistry at the outer surface of VPs. In other words, whether wild or synthetic VPs and regardless of the scientific fields involved, taming viruses implies thus managing the physical chemistry at the VP external surface. The surface hydrophobicity (SH) of VPs is a critical feature that must be looked at. Still, the literature dealing with nanoscale hydrophobic domains at the proteinaceous surface of VPs underlying their global SH is like a fragmented puzzle. This article provides an overview of the topic from the perspective of modern protein biophysics for updating the classic physicochemical picture of outer VP/water interfaces hitherto accepted. Patterns of non-polar and \"false-polar\" patches, expressing variable hydrophobic degrees according to neighboring polar patches, are now drawn. The extensive discussion of reviewed data generates such fresh ideas to explore in the coming years for better modeling the SH of wild virions or engineered virus-based nanoparticles, paving the way for new directions in fundamental virology and virus-based chemistry.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400221"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0