Advanced biology最新文献_第2页

CBX3 Downregulates HLTF to Activate PI3K/AKT Signaling Promoting Cholangiocarcinoma. CBX3 下调 HLTF 激活 PI3K/AKT 信号促进胆管癌的发生

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-11-27 DOI: 10.1002/adbi.202400413

Min Xie, Huaiyuan Liang, Yuxuan Mao, Yuping Yao, Bingzhang Tian

引用次数: 0

Inhibiting DNA Sensing Pathway Controls Steroid Hyporesponsive Lung Inflammation. 抑制DNA传感通路可控制类固醇低反应性肺部炎症

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-11-27 DOI: 10.1002/adbi.202400230

Bushra Mdkhana, Narjes Saheb Sharif-Askari, Roberta Cagliani, Baraa Khalid Saleh Al-Sheakly, Rakhee K Ramakrishnan, Fatemeh Saheb Sharif-Askari, Ibrahim Yaseen Hachim, Qutayba Hamid, Mutasem Rawas-Qalaji, Rabih Halwani

{"title":"Inhibiting DNA Sensing Pathway Controls Steroid Hyporesponsive Lung Inflammation.","authors":"Bushra Mdkhana, Narjes Saheb Sharif-Askari, Roberta Cagliani, Baraa Khalid Saleh Al-Sheakly, Rakhee K Ramakrishnan, Fatemeh Saheb Sharif-Askari, Ibrahim Yaseen Hachim, Qutayba Hamid, Mutasem Rawas-Qalaji, Rabih Halwani","doi":"10.1002/adbi.202400230","DOIUrl":"10.1002/adbi.202400230","url":null,"abstract":"DNA damage underlies the progression of asthma toward a severe, steroid hyporesponsive phenotype. The accumulation of double-stranded DNA within the cytosol triggers the activation of cytosolic DNA-sensing pathways, notably the Stimulator of Interferon Genes (STING) pathway. However, the precise role of STING in driving steroid hyporesponsiveness remains elusive and warrants further investigation. This study evaluates STING levels in human bronchial fibroblasts from severe asthmatic patients and in lung homogenates from a steroid hyporesponsive lung inflammation mouse model. STING level is assessed at baseline, post house dust mites (HDM) stimulation, and following treatment with dexamethasone and STING inhibitor. The effect of STING inhibitors on regulating steroid hyporesponsiveness particularly glucocorticoid receptor (GR)-α/GR-β ratio is also examined. Severe asthmatic fibroblasts exhibit elevated STING/IFN-I pathway activation, further heightened by HDM and a similar pattern is seen in lung homogenates from steroid hyporesponsive mice. Dexamethasone combined with an STING inhibitor reduces STING activity, while dexamethasone alone is ineffective. Interestingly, the STING inhibitor restores steroid sensitivity by increasing the GRα/GRβ ratio. Furthermore, nanoparticle-encapsulated STING inhibitor more effectively reduces airway hyperresponsiveness and restores steroid sensitivity than the free inhibitor. These findings emphasize STING's role in severe asthma pathogenesis, proposing nanoparticle delivery of STING inhibitors as a promising therapeutic strategy.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400230"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Action of Tongjiang Mixture for Treating Reflux Esophagitis: A Study Using Serum Pharmacochemistry and Network Pharmacology. 通江合剂治疗反流性食管炎的作用机制：血清药物化学和网络药理学研究。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-11-27 DOI: 10.1002/adbi.202400187

Yang Lu, Huang Yuzhen, Gu Yi, Wu Lili, Wang Yan, Tao Weiwei, Liu Wanli

{"title":"Mechanism of Action of Tongjiang Mixture for Treating Reflux Esophagitis: A Study Using Serum Pharmacochemistry and Network Pharmacology.","authors":"Yang Lu, Huang Yuzhen, Gu Yi, Wu Lili, Wang Yan, Tao Weiwei, Liu Wanli","doi":"10.1002/adbi.202400187","DOIUrl":"10.1002/adbi.202400187","url":null,"abstract":"Tongjiang Mixture (TJM) is a traditional Chinese formula for treating reflux esophagitis (RE). Nevertheless, its active ingredients and potential pharmacological mechanisms are not yet clearly elucidated. This study will identify the active ingredients of TJM using serum pharmacochemistry and to elucidate the mechanism on RE through network pharmacology. The blood-borne ingredients of TJM are identified by the Ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometer. Subsequently, a \"compound-target-disease\" network is established and obtained core targets associated with TJM and RE. Then, the potential signaling pathways are forecasted through the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Finally, the rat model of RE is established to verify the results predicted by network pharmacology through animal experiments. Fifteen blood-borne ingredients of TJM are identified, with eight active ingredients-namely Tangeretin, Tricin, Palmati, Berberine, Limonin, Evodiamine, Tetrahydropalmatine and Rutecarpine - making significant contributions to its efficacy. Moreover, TJM is predicted to act on 193 targets related to RE, involving AKT1, HSP90AA1, PIK3CA, and other targets, which enriches mainly in PI3K/AKT /NF-κB signaling. Additionally, TJM can alleviate inflammation of the esophageal mucosa, reduce pathological damage, and increase gastric pH. It can downregulate PI3K, AKT, and NF-κB mRNA transcription levels and reduce the protein expression of PI3K, AKT, and NF-κB. Furthermore, it can inhibit the overproduction of IL-6, TNF-α and IL-17. TJM can alleviate immune-inflammatory responses and ameliorate RE by restraining the PI3K/AKT pathway and its downstream NF-κB.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400187"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Method for Fabricating Tissue-Specific Extracellular Matrix Blocks From Decellularized Tissue Powders. 利用脱细胞组织粉末制造组织特异性细胞外基质块的方法。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-11-27 DOI: 10.1002/adbi.202400398

Jun Negishi, Ayana Yamaguchi, Dan Tanaka, Yoshihide Hashimoto, Yongwei Zhang, Seiichi Funamoto

引用次数: 0

PAK4 is Required for Meiotic Resumption, Spindle Assembly, and Cortical Migration in Mouse Oocytes During Meiotic Maturation. PAK4是小鼠卵母细胞在减数分裂成熟过程中恢复减数分裂、纺锤体组装和皮质迁移所必需的。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-10-22 DOI: 10.1002/adbi.202400307

Ke Song, Dandan Chen, Jingyu Li, Jiaqi Zhang, Ying Tian, Xiangning Xu, Bicheng Wang, Ziqi Huang, Shuo Lou, Jingyi Kang, Ningning Zhang, Xiaokui Yang, Wei Ma

{"title":"PAK4 is Required for Meiotic Resumption, Spindle Assembly, and Cortical Migration in Mouse Oocytes During Meiotic Maturation.","authors":"Ke Song, Dandan Chen, Jingyu Li, Jiaqi Zhang, Ying Tian, Xiangning Xu, Bicheng Wang, Ziqi Huang, Shuo Lou, Jingyi Kang, Ningning Zhang, Xiaokui Yang, Wei Ma","doi":"10.1002/adbi.202400307","DOIUrl":"10.1002/adbi.202400307","url":null,"abstract":"Oocyte meiotic errors can cause infertility, miscarriage, and birth defects. Here the role and the underlying mechanism of p21 activated kinase 4 (PAK4) in mouse oocyte meiosis is evaluated. It is found that PAK4 expression and its phosphorylation are detected in high level at germinal vesicle (GV) stage, and gradually decreased after meiotic resumption in oocytes. PAK4 has direct physical interaction with both mitogen-activated protein kinases 1/2 (MEK1/2) and Paxillin, they are colocalized on the spindle structure during metaphases I and II. Phospho-PAK4 is distributed beneath the cytoplasmic membrane and on the chromosomes, and colocalized with the microtubule organizing center (MTOC) proteins, Pericentrin and γ-tubulin, as well as phosphor-MEK1/2 and phosphor-Paxillin on spindle poles. PAK4 inhibition by chemical inhibitor LCH-7749944, specific Pak4 morpholino oligo or the dominant negative mutant Pak4K350, 351 M influence the meiotic resumption, spindle assembly and its cortical migration, and associated with the downregulation in the dephosphorylation of cyclin dependent kinase 1 (CDK1) and the levels of Cyclin B1, MEK1/2, Paxillin, g-tubulin, acetylated a-tubulin, Arp3, and Cofilin phosphorylation in oocytes. In sum, PAK4 functions to sustain the rational levels of Cyclin B1, MEK1/2, Paxillin, y-tubulin, acetylated a-tubulin, Arp3, and phosphor-Cofilin in mouse oocytes, thereby promotes the meiotic resumption, spindle assembly, and migration during meiotic maturation.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400307"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospective Intervention Strategies Between Skeletal Muscle Health and Mitochondrial Changes During Aging. 老化过程中骨骼肌健康与线粒体变化之间的前瞻性干预策略。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1002/adbi.202400235

Xin Zhang, Suchan Liao, Lingling Huang, Jinhua Wang

{"title":"Prospective Intervention Strategies Between Skeletal Muscle Health and Mitochondrial Changes During Aging.","authors":"Xin Zhang, Suchan Liao, Lingling Huang, Jinhua Wang","doi":"10.1002/adbi.202400235","DOIUrl":"10.1002/adbi.202400235","url":null,"abstract":"Sarcopenia is a geriatric condition characterized by a decrease in skeletal muscle mass and function, significantly impacting both quality of life and overall health. Mitochondria are the main sites of energy production within the cell, and also produce reactive oxygen species (ROS), which maintain mitochondrial homeostasis-mitophagy (clearing damaged mitochondria); mitochondrial dynamics, which involve fusion and fission to regulate mitochondrial morphology; mitochondrial biogenesis, which ensures the functionality and homeostasis of mitochondria. Sarcopenia is linked to mitochondrial dysfunction, suggesting that muscle mitochondrial function therapy should be investigated. Extrinsic therapies are extensively examined to identify new treatments for muscular illnesses including sarcopenia. Changes in muscle physiology and lifestyle interventions, such as pharmacological treatments and exercise, can modulate mitochondrial activity in older adults. This PubMed review encompasses the most significant mitophagy and sarcopenia research from the past five years. Animal models, cellular models, and human samples are well covered. The review will inform the development of novel mitochondria-targeted therapies aimed at combating age-related muscle atrophy.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400235"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel UBM/SIS Composite Biological Scaffold for 2-Year Abdominal Defect Repairing and Strength Recovery in Canine Model. 一种新型 UBM/SIS 复合生物支架，用于犬模型腹部缺损修复和力量恢复两年。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-11-14 DOI: 10.1002/adbi.202400131

Weidong Zhong, Jinshui Chen, Qifeng Xie, Wenyue Cheng, Meibiao Zhao, Yang Sun, Jing Dai, Jian Zhang

{"title":"A Novel UBM/SIS Composite Biological Scaffold for 2-Year Abdominal Defect Repairing and Strength Recovery in Canine Model.","authors":"Weidong Zhong, Jinshui Chen, Qifeng Xie, Wenyue Cheng, Meibiao Zhao, Yang Sun, Jing Dai, Jian Zhang","doi":"10.1002/adbi.202400131","DOIUrl":"10.1002/adbi.202400131","url":null,"abstract":"Biological scaffolds are widely utilized in hernia treatment due to their exceptional pro-regenerative properties, which mitigate scar formation. However, serious complications occurred, caused by inflammatory response, premature degradation, and mechanical failure. Consequently, improvements of the biological scaffold are necessary to mitigate these risks. In this study, a novel biological scaffold integrating basement membrane-containing urinary bladder matrix (UBM) and small intestinal submucosa (SIS) is developed, and its safety and effectiveness are assessed in comparison to a commercial SIS (c-SIS) scaffold. The introduction of UBM as top surface layers significantly promotes cell adhesion, facilitating rapid formation of isolated regeneration zone. Proteomic analysis has demonstrated a more efficient decellularization of the UBM/SIS scaffold, which subsequently mitigates inflammation in murine models, and promotes the polarization of macrophages toward the pro-healing M2 phenotype in a rat model of abdominal wall muscle defect. Furthermore, a two-year repair trial is conducted on a full-thickness abdominal wall muscle defect in canine model and confirmed that the UBM/SIS scaffold exhibits reduced seroma occurrences and enhanced tissue repair performances. Overall, the efficacy of this novel biological scaffold suggests its potential to minimize hernia recurrence in clinical practice and mitigate patient suffering from severe inflammatory responses.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400131"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Anti-Inflammatory and Immunosuppressant Potential of Isotelekin in Lipopolysaccharide (LPS) Stimulated Macrophage (RAW 264.7) and Sheep Red Blood Cells (SRBC) Sensitized Murine Models. 评估异特勒金在脂多糖（LPS）刺激的巨噬细胞（RAW 264.7）和绵羊红细胞（SRBC）致敏小鼠模型中的抗炎和免疫抑制潜力。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI: 10.1002/adbi.202400386

Irfan Qasam, Shah Nawaz, Hema Kumari, Narendra Chauhan, Yedukondalu Nalli, Govind Yadav

{"title":"Evaluation of Anti-Inflammatory and Immunosuppressant Potential of Isotelekin in Lipopolysaccharide (LPS) Stimulated Macrophage (RAW 264.7) and Sheep Red Blood Cells (SRBC) Sensitized Murine Models.","authors":"Irfan Qasam, Shah Nawaz, Hema Kumari, Narendra Chauhan, Yedukondalu Nalli, Govind Yadav","doi":"10.1002/adbi.202400386","DOIUrl":"10.1002/adbi.202400386","url":null,"abstract":"The present study explored the natural compound Isotelekin isolated from Inula racemose against anti-inflammatory and immunomodulatory potential in LPS-induced RAW264.7 cell lines and immune-elevated SRBC-sensitized animal models. Isotelekin in in vitro studies, inhibited the production of Th-1 cytokines Interleukin-6 (IL-6), Tumour necrosis factor (TNF-α), and Interferon-gamma (INF-γ), and increased Th-2 cytokines Interleukin-10 (IL-10). Whereas it inhibited the nitrites and reactive oxygen species (ROS) production by mitigating the effect of LPS significantly. In vivo immunomodulatory activity in Delayed-type hypersensitivity (DTH) and Hemagglutinating antibody (HA), Isotelekin suppressed the cellular as well as humoral immunity in immune-affected and SRBC-sensitized mice. Isotelekin decreased the phagocytic responses against carbon particles and plaque-forming mainly IgG (Immunoglobulin G) production. Additionally, Isotelekin showed immunosuppressive potential through the evaluation of splenocytes, allograft acceptance, and haematological parameters. Molecular studies, including western blot analysis and immunocytochemistry, revealed that Isotelekin reduced the expression of iNOS (Inducible nitric oxide synthase), COX-2 (Cyclo-Oxygenase 2), and p-IkBα (Phospho I-kappa-B-alpha), and significantly inhibited the nuclear translocation of NF-κB/p65. Based on these results, Isotelekin at 10 µm in in vitro and at 30 mg kg-1 in in vivo demonstrated strong anti-inflammatory and immunosuppressive therapeutic potential.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400386"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential for Extracellular Vesicles in Nanomedicine: A Review of Recent Advancements and Challenges Ahead. 细胞外囊泡在纳米医学中的潜力：近期进展和未来挑战的综述。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-31 DOI: 10.1002/adbi.202400623

Farbod Ebrahimi, Anjali Kumari, Samaneh Ghadami, Saqer Al Abdullah, Kristen Dellinger

{"title":"The Potential for Extracellular Vesicles in Nanomedicine: A Review of Recent Advancements and Challenges Ahead.","authors":"Farbod Ebrahimi, Anjali Kumari, Samaneh Ghadami, Saqer Al Abdullah, Kristen Dellinger","doi":"10.1002/adbi.202400623","DOIUrl":"https://doi.org/10.1002/adbi.202400623","url":null,"abstract":"Extracellular vesicles (EVs) have emerged as promising tools in diagnostics and therapy for chronic diseases, including cancer and Alzheimer's. Small EVs, also called exosomes, are lipid-bound particles (≈30-150 nm) that play a role in healthy and pathophysiological interactions, including intercellular communication, by transporting bioactive molecules, including proteins, lipids, and nucleic acids. Their ability to cross biological barriers, such as the blood-brain barrier, makes them ideal candidates for targeted therapeutic interventions. In the context of chronic diseases, exosomes can be engineered to deliver active agents, including small molecules and siRNAs to specific target cells, providing a novel approach to precision medicine. Moreover, exosomes show great promise as repositories for diagnostic biomarkers. Their cargo can reflect the physiological and pathological status of the parent cells, making them valuable indicators of disease progression and response to treatment. This paper presents a comprehensive review of the application of exosomes in four chronic diseases: cancer, cardiovascular disease, neurodegenerative disease, and orthopedic disease, which significantly impact global public health due to their high prevalence and associated morbidity and mortality rates. Furthermore, the potential of exosomes as valuable tools for theranostics and disease management is highlighted. Finally, the challenges associated with exosomes and their demonstrated potential for advancing future nanomedicine applications are discussed.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400623"},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysozyme/Tracheal Antimicrobial Peptide-Based Tissue-Specific Expression Antimicrobial Plasmids Show Broad-Spectrum Antibacterial Activities in the Treatment of Mastitis in Mice. 基于溶菌酶/气管抗菌肽的组织特异性表达抗菌质粒在治疗小鼠乳腺炎中显示出广谱抗菌活性。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-12-30 DOI: 10.1002/adbi.202400132

Yi Yang, Yining Meng, Daijie Chen, Ping Hou, Zhipeng Zhang, Wenqiang Cao, Ye Meng, Qianwen Zhang, Runyan Tu, Xiaoli Hao, Aijian Qin, Shaobin Shang, Zhangping Yang

{"title":"Lysozyme/Tracheal Antimicrobial Peptide-Based Tissue-Specific Expression Antimicrobial Plasmids Show Broad-Spectrum Antibacterial Activities in the Treatment of Mastitis in Mice.","authors":"Yi Yang, Yining Meng, Daijie Chen, Ping Hou, Zhipeng Zhang, Wenqiang Cao, Ye Meng, Qianwen Zhang, Runyan Tu, Xiaoli Hao, Aijian Qin, Shaobin Shang, Zhangping Yang","doi":"10.1002/adbi.202400132","DOIUrl":"https://doi.org/10.1002/adbi.202400132","url":null,"abstract":"The use of antibiotics is the preferred therapy for bacterial diseases. However, overusing antibiotics has led to the development of antibiotic resistance in bacteria, which is now a major public health concern. Therefore, in this study, the performance of lysozyme (LYZ)/tracheal antimicrobial peptide (TAP)-based tissue-specific expression antimicrobial plasmids (TSEAP) have been evaluated in the treatment of mastitis in mice. The results show that LYZ/ and TAP-based TSEAP could effectively reduce the clinical symptoms caused by Staphylococcus sciuri, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa-induced mastitis. In addition, the studies of behavioral tests, parameters of weight growth, blood biochemistry, and organ coefficients comprehensively indicate that the transfection of LYZ/TAP-based TSEAP is safe in mice. Taken together, LYZ/TAP-based TSEAP have broad-spectrum antibacterial activity and may provide new insight for the non-antibiotic treatment of bacterial diseases.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400132"},"PeriodicalIF":3.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0