Advanced biology最新文献_第2页

Therapeutic Osteoinductive Potential of Saposhnikovia divaricata Schischkin in a Mouse Drill-Hole Bone Defect Model 鼠尾草对小鼠钻孔骨缺损模型的骨诱导作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.70149

Hyunseong Kim, Jin Young Hong, Hyun Kim, Changhwan Yeo, Wan-Jin Jeon, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha

{"title":"Therapeutic Osteoinductive Potential of Saposhnikovia divaricata Schischkin in a Mouse Drill-Hole Bone Defect Model","authors":"Hyunseong Kim, Jin Young Hong, Hyun Kim, Changhwan Yeo, Wan-Jin Jeon, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha","doi":"10.1002/adbi.70149","DOIUrl":"https://doi.org/10.1002/adbi.70149","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone fracture healing requires coordinated osteoclast-mediated resorption and osteoblast-driven formation. <i>Saposhnikovia divaricata</i> Schischkin (SDS) is a traditional East Asian medicine with anti-inflammatory and immunomodulatory activities, but its role in bone regeneration remains unclear. This study evaluated the osteogenic potential of SDS using integrated in vitro, in vivo, and in silico approaches. Mouse-derived osteoblasts were treated with SDS, and cell viability, alkaline phosphatase (ALP) activity, and mineralization were assessed by CCK-8, ALP assay, and Alizarin Red O staining. A femoral drill-hole injury model was established in C57BL/6 mice, followed by oral administration of SDS (100, 200, or 400 mg/kg). Bone healing was evaluated by micro-computed tomography and histological analyses. SDS enhanced osteoblast viability, ALP activity, mineral deposition, and osteogenic marker expression in vitro. In vivo, SDS increased bone volume fraction and bone mineral density at 1 week after injury, accompanied by reduced TRAP-positive osteoclasts and increased osteocalcin staining. Molecular docking predicted favorable interactions between major chromone-derived constituents of SDS and the bone-related proteins RUNX2 and RANK. Collectively, these findings suggest that SDS may promote cellular processes associated with early bone repair and warrant further investigation into its mechanisms and long-term therapeutic potential.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Analyses Reveal Dysregulation of Ribosomal Protein Genes During Hematopoietic Stem and Progenitor Cell Aging 单细胞分析揭示了造血干细胞和祖细胞衰老过程中核糖体蛋白基因的失调

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.70150

Roger Atanga, Saurav Mallik, Soumita Seth, Francine Grodstein, David A Bennett, Bernardo Lemos

{"title":"Single-Cell Analyses Reveal Dysregulation of Ribosomal Protein Genes During Hematopoietic Stem and Progenitor Cell Aging","authors":"Roger Atanga, Saurav Mallik, Soumita Seth, Francine Grodstein, David A Bennett, Bernardo Lemos","doi":"10.1002/adbi.70150","DOIUrl":"https://doi.org/10.1002/adbi.70150","url":null,"abstract":"<div>\u0000 \u0000 <p>Hematopoietic stem and progenitor cells (HSPCs) sustain lifelong blood production, yet the molecular mechanisms underlying their functional decline with age remain incompletely understood. Understanding how aging alters the transcriptomic landscape of HSPCs is critical to uncovering the origins of immune system aging. We performed a comprehensive single-cell RNA sequencing analysis integrating over 300,000 bone marrow-derived HSPCs from 50 healthy individuals spanning 19 to 84 years of age. Aging was associated with immune lineage skewing, marked by increased myeloid and decreased lymphoid output in both bone marrow and peripheral blood. Subtle increases in HSCs, MEPs, and myeloid progenitors alongside reductions in lymphoid progenitors were already evident in aged bone marrow, suggesting that lineage bias is encoded at the progenitor level. Age-associated transcriptional changes included extensive upregulation of ribosomal genes encoding small (RPS11, RPS12, RPS23) and large (RPL9, RPL19, RPL24) cytoplasmic ribosomal subunit proteins, as well as pro-inflammatory mediators (IL1B, IL18, TGFB1, S100A8). Enrichment analysis identified mitochondrial function, ribosome biogenesis, chromatin remodeling, and inflammatory signaling as key ontologies disrupted during HSPC aging. Our study identifies molecular signatures of systemic aging rooted in bone marrow HSPCs and suggests that dysregulated ribosomal protein gene expression is an under-appreciated hallmark of hematopoietic stem cell aging.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Role of EMR1 in Lymph Nodes and Primary Tumors of Colon Cancer Patients EMR1在结肠癌患者淋巴结和原发肿瘤中的预后作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.202500645

Tamer Roshdy, Hagar Eltorky, Alaa Mohamed, Faten Zahran, Adel Guirgis, Lina Olsson, Gudrun Lindmark, Marie-Louise Hammarström, Sten Hammarström, Basel Sitohy

{"title":"Prognostic Role of EMR1 in Lymph Nodes and Primary Tumors of Colon Cancer Patients","authors":"Tamer Roshdy, Hagar Eltorky, Alaa Mohamed, Faten Zahran, Adel Guirgis, Lina Olsson, Gudrun Lindmark, Marie-Louise Hammarström, Sten Hammarström, Basel Sitohy","doi":"10.1002/adbi.202500645","DOIUrl":"https://doi.org/10.1002/adbi.202500645","url":null,"abstract":"<p>The purpose was to investigate whether the eosinophil biomarker EMR1, ectopically expressed in colon cancer tumor cells, could be used to identify patients at risk for relapse after curative surgery. EMR1 mRNA levels were determined in primary tumors (<i>n</i> = 66) and lymph nodes (LNs; <i>n</i> = 377) from 121 colon cancer patients. Controls were resection margins of colon cancer tumors and LNs from patients with inflammatory bowel disease. Primary read-out was recurrency evaluated as disease-free survival time in the Kaplan–Meier survival model and hazard ratio calculation by uni- and multivariate Cox regression. High EMR1 levels in the regional LNs were associated with poor prognosis [hazard ratio 3.1 (1.2–8.0) at 5-year follow-up, <i>p</i> = 0.02]. Patients with a very poor prognosis were identified by combining EMR1 with CXCL17 analysis of LNs [hazard ratio 4.6 (1.1–19.7), <i>p</i> = 0.04]. High EMR1 levels in the primary tumor were associated with a good prognosis [hazard ratio 0.1 (0.02–0.9), <i>p</i> = 0.04]. Multivariate analysis strengthened the result for LNs but weakened the result for the primary tumor. These contradictory prognostic results probably relate to the prominent eosinophilia in the primary tumor, essentially lacking in LNs, where ectopically expressed EMR1 in tumor cells dominates. Thus, EMR1 expressed in tumor cells probably promotes tumor growth.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Role of EMR1 in Lymph Nodes and Primary Tumors of Colon Cancer Patients EMR1在结肠癌患者淋巴结和原发肿瘤中的预后作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.202500645

Tamer Roshdy, Hagar Eltorky, Alaa Mohamed, Faten Zahran, Adel Guirgis, Lina Olsson, Gudrun Lindmark, Marie-Louise Hammarström, Sten Hammarström, Basel Sitohy

{"title":"Prognostic Role of EMR1 in Lymph Nodes and Primary Tumors of Colon Cancer Patients","authors":"Tamer Roshdy, Hagar Eltorky, Alaa Mohamed, Faten Zahran, Adel Guirgis, Lina Olsson, Gudrun Lindmark, Marie-Louise Hammarström, Sten Hammarström, Basel Sitohy","doi":"10.1002/adbi.202500645","DOIUrl":"https://doi.org/10.1002/adbi.202500645","url":null,"abstract":"<p>The purpose was to investigate whether the eosinophil biomarker EMR1, ectopically expressed in colon cancer tumor cells, could be used to identify patients at risk for relapse after curative surgery. EMR1 mRNA levels were determined in primary tumors (<i>n</i> = 66) and lymph nodes (LNs; <i>n</i> = 377) from 121 colon cancer patients. Controls were resection margins of colon cancer tumors and LNs from patients with inflammatory bowel disease. Primary read-out was recurrency evaluated as disease-free survival time in the Kaplan–Meier survival model and hazard ratio calculation by uni- and multivariate Cox regression. High EMR1 levels in the regional LNs were associated with poor prognosis [hazard ratio 3.1 (1.2–8.0) at 5-year follow-up, <i>p</i> = 0.02]. Patients with a very poor prognosis were identified by combining EMR1 with CXCL17 analysis of LNs [hazard ratio 4.6 (1.1–19.7), <i>p</i> = 0.04]. High EMR1 levels in the primary tumor were associated with a good prognosis [hazard ratio 0.1 (0.02–0.9), <i>p</i> = 0.04]. Multivariate analysis strengthened the result for LNs but weakened the result for the primary tumor. These contradictory prognostic results probably relate to the prominent eosinophilia in the primary tumor, essentially lacking in LNs, where ectopically expressed EMR1 in tumor cells dominates. Thus, EMR1 expressed in tumor cells probably promotes tumor growth.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Analyses Reveal Dysregulation of Ribosomal Protein Genes During Hematopoietic Stem and Progenitor Cell Aging 单细胞分析揭示了造血干细胞和祖细胞衰老过程中核糖体蛋白基因的失调

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.70150

Roger Atanga, Saurav Mallik, Soumita Seth, Francine Grodstein, David A Bennett, Bernardo Lemos

{"title":"Single-Cell Analyses Reveal Dysregulation of Ribosomal Protein Genes During Hematopoietic Stem and Progenitor Cell Aging","authors":"Roger Atanga, Saurav Mallik, Soumita Seth, Francine Grodstein, David A Bennett, Bernardo Lemos","doi":"10.1002/adbi.70150","DOIUrl":"https://doi.org/10.1002/adbi.70150","url":null,"abstract":"<div>\u0000 \u0000 <p>Hematopoietic stem and progenitor cells (HSPCs) sustain lifelong blood production, yet the molecular mechanisms underlying their functional decline with age remain incompletely understood. Understanding how aging alters the transcriptomic landscape of HSPCs is critical to uncovering the origins of immune system aging. We performed a comprehensive single-cell RNA sequencing analysis integrating over 300,000 bone marrow-derived HSPCs from 50 healthy individuals spanning 19 to 84 years of age. Aging was associated with immune lineage skewing, marked by increased myeloid and decreased lymphoid output in both bone marrow and peripheral blood. Subtle increases in HSCs, MEPs, and myeloid progenitors alongside reductions in lymphoid progenitors were already evident in aged bone marrow, suggesting that lineage bias is encoded at the progenitor level. Age-associated transcriptional changes included extensive upregulation of ribosomal genes encoding small (RPS11, RPS12, RPS23) and large (RPL9, RPL19, RPL24) cytoplasmic ribosomal subunit proteins, as well as pro-inflammatory mediators (IL1B, IL18, TGFB1, S100A8). Enrichment analysis identified mitochondrial function, ribosome biogenesis, chromatin remodeling, and inflammatory signaling as key ontologies disrupted during HSPC aging. Our study identifies molecular signatures of systemic aging rooted in bone marrow HSPCs and suggests that dysregulated ribosomal protein gene expression is an under-appreciated hallmark of hematopoietic stem cell aging.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Osteoinductive Potential of Saposhnikovia divaricata Schischkin in a Mouse Drill-Hole Bone Defect Model 鼠尾草对小鼠钻孔骨缺损模型的骨诱导作用

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-14 DOI: 10.1002/adbi.70149

Hyunseong Kim, Jin Young Hong, Hyun Kim, Changhwan Yeo, Wan-Jin Jeon, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha

{"title":"Therapeutic Osteoinductive Potential of Saposhnikovia divaricata Schischkin in a Mouse Drill-Hole Bone Defect Model","authors":"Hyunseong Kim, Jin Young Hong, Hyun Kim, Changhwan Yeo, Wan-Jin Jeon, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha","doi":"10.1002/adbi.70149","DOIUrl":"https://doi.org/10.1002/adbi.70149","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone fracture healing requires coordinated osteoclast-mediated resorption and osteoblast-driven formation. <i>Saposhnikovia divaricata</i> Schischkin (SDS) is a traditional East Asian medicine with anti-inflammatory and immunomodulatory activities, but its role in bone regeneration remains unclear. This study evaluated the osteogenic potential of SDS using integrated in vitro, in vivo, and in silico approaches. Mouse-derived osteoblasts were treated with SDS, and cell viability, alkaline phosphatase (ALP) activity, and mineralization were assessed by CCK-8, ALP assay, and Alizarin Red O staining. A femoral drill-hole injury model was established in C57BL/6 mice, followed by oral administration of SDS (100, 200, or 400 mg/kg). Bone healing was evaluated by micro-computed tomography and histological analyses. SDS enhanced osteoblast viability, ALP activity, mineral deposition, and osteogenic marker expression in vitro. In vivo, SDS increased bone volume fraction and bone mineral density at 1 week after injury, accompanied by reduced TRAP-positive osteoclasts and increased osteocalcin staining. Molecular docking predicted favorable interactions between major chromone-derived constituents of SDS and the bone-related proteins RUNX2 and RANK. Collectively, these findings suggest that SDS may promote cellular processes associated with early bone repair and warrant further investigation into its mechanisms and long-term therapeutic potential.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterning of Multilayer Biointerfaces Driven by Microbubble Lithography Toward Optical Biochips 面向光学生物芯片的微泡光刻驱动多层生物界面图像化。

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-06 DOI: 10.1002/adbi.202500612

Anand Dev Ranjan, Suyash Narayan Amzare, Subhrokoli Ghosh, Ayan Banerjee

{"title":"Patterning of Multilayer Biointerfaces Driven by Microbubble Lithography Toward Optical Biochips","authors":"Anand Dev Ranjan, Suyash Narayan Amzare, Subhrokoli Ghosh, Ayan Banerjee","doi":"10.1002/adbi.202500612","DOIUrl":"10.1002/adbi.202500612","url":null,"abstract":"<div>\u0000 \u0000 <p>The precise and non-invasive integration of biological molecules into engineered surfaces remains a critical challenge in the development of advanced biointerfaces for biophotonics applications. Traditional fabrication techniques often rely on harsh chemical processes or photolithographic steps that risk compromising biomolecular function and lack microscale spatial selectivity. In this context, Microbubble Lithography (MBL) has established itself as an optical tweezers driven microbubble-based mask-free route toward patterning of biointerfaces in order to fabricate three-layer biointerfaces entirely in situ. This work reinforces this technology further and provides important advances in the form of quantitative understanding of the controlled patterning of fluorescent reporters on a heterostructured interface enhanced by an absorbing substrate and a cross-linker to improve interfacial stability and signal-to-noise ratios. We address important questions regarding adherence, reporter and cross-linker concentrations, while also studying the science behind the formation of such heterostructures. Thus, we explain the observed twin-peak, edge-dominated fluorescence via a phenomenological model using central thermophoretic depletion and peripheral enrichment at the three-phase contact line (TPCL), and a 3D fluorescence surface visualizing peripheral enrichment. Together, these results establish MBL as a mask-free, chemically gentle, and highly controllable route to construct an optical biochip platform to fabricate biomolecular architectures for biophotonics and biosensing applications.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Platform for Fast and Cost-Effective Screening of Immunomodulatory Compounds 一种快速、低成本筛选免疫调节化合物的新平台

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.202500696

Suzana Lj. Popovic, Dejan D. Baskic, Marina M. Mitrovic, Sanja D. Matic, Tijana R. Markovic, Marijana S. Stanojevic Pirkovic, Mirela M. Jevtic, Ivana Z. Lazic, Danijela V. Todorovic

{"title":"A Novel Platform for Fast and Cost-Effective Screening of Immunomodulatory Compounds","authors":"Suzana Lj. Popovic, Dejan D. Baskic, Marina M. Mitrovic, Sanja D. Matic, Tijana R. Markovic, Marijana S. Stanojevic Pirkovic, Mirela M. Jevtic, Ivana Z. Lazic, Danijela V. Todorovic","doi":"10.1002/adbi.202500696","DOIUrl":"https://doi.org/10.1002/adbi.202500696","url":null,"abstract":"<div>\u0000 \u0000 <p>Compounds with immunomodulatory properties are increasingly recognized as valuable adjunctive therapies in pathological conditions associated with immune dysregulation. However, their discovery process is often costly and time-consuming. The aim of this study is to develop a low-cost, simple-to-perform, and reliable method for screening immunomodulatory agents. Human peripheral blood mononuclear cells (PBMNCs) are used as a model system. PBMNCs are isolated by density gradient centrifugation and treated with different immunostimulators and immunosuppressors. By measuring several analytes with a gas analyzer, it is revealed that changes in lactate levels alone can be used as an indicator of immune cell activation/suppression. This finding is further confirmed by measuring the expression of cell surface activation markers and HIF1α using flow cytometry, glucose concentration in PBMNC cultures using a gas analyzer, and levels of TNF-ά and IL-10 by ELISA. The results presented here reveal the simplicity and reliability of this innovative platform for screening immunomodulatory compounds.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of GHSRs Facilitates the Excitability of Dentate Gyrus Granule Cells and Short-Term Spatial Memory via Multiple Ionic and Signaling Mechanisms GHSRs的激活通过多种离子和信号机制促进齿状回颗粒细胞的兴奋性和短期空间记忆

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.70145

Chidiebele S. Oraegbuna, Phani K. Kola, Simon L. Griggs, Saobo Lei

{"title":"Activation of GHSRs Facilitates the Excitability of Dentate Gyrus Granule Cells and Short-Term Spatial Memory via Multiple Ionic and Signaling Mechanisms","authors":"Chidiebele S. Oraegbuna, Phani K. Kola, Simon L. Griggs, Saobo Lei","doi":"10.1002/adbi.70145","DOIUrl":"https://doi.org/10.1002/adbi.70145","url":null,"abstract":"<p>Ghrelin is the endogenous ligand of the ghrelin secretagogue receptors (GHSRs). Although the GHSRs are densely expressed in the dentate gyrus (DG) granule cells (GCs), their roles in the DG GCs remain to be fully understood. We probed the actions of ghrelin on the DG GCs with whole-cell recordings and its effects on short-term spatial memory using the Y-maze spontaneous alternation test. Application of ghrelin excited the DG GCs by depressing the G protein-coupled inwardly rectifying K<sup>+</sup> (GIRK) channels and ATP-sensitive K<sup>+</sup> (K<sub>ATP</sub>) channels, activating the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and a TTX-resistant, persistent Na<sup>+</sup> channel. Adenylyl cyclase and EPAC2 were required, whereas phospholipase C and protein kinase A were not needed for the ghrelin-mediated excitation of DG GCs. Overnight fasting augmented the excitability of the DG GCs via activation of GHSRs, suggesting a role for endogenously released ghrelin. Ghrelin also enhanced synaptic recruitment of action potentials in DG GCs. Microinjection of ghrelin into the DG increased the alternating sequences in the Y maze test via GHSR-mediated elevation of cAMP and EPAC signals. Our results provide an action mode to explain the roles of GHSRs in improving cognitive functions.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA-XIST Competitively Binds to MicroRNA-532-5p to Regulate PTEN Expression, Exacerbating Cerebral Ischemia-Reperfusion Injury LncRNA-XIST竞争性结合MicroRNA-532-5p调控PTEN表达，加重脑缺血-再灌注损伤

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.202500593

Xudong Lu, Luhan Chen, Liqi Qian, Xiaoling Zhang, Yanping Wang, Bo Yu

{"title":"LncRNA-XIST Competitively Binds to MicroRNA-532-5p to Regulate PTEN Expression, Exacerbating Cerebral Ischemia-Reperfusion Injury","authors":"Xudong Lu, Luhan Chen, Liqi Qian, Xiaoling Zhang, Yanping Wang, Bo Yu","doi":"10.1002/adbi.202500593","DOIUrl":"https://doi.org/10.1002/adbi.202500593","url":null,"abstract":"<div>\u0000 \u0000 <p>Cerebral ischemia-reperfusion injury (CIRI) is a severe neurological condition. LncRNA-XIST is closely associated with CIRI; however, its underlying mechanism remains unclear. A middle cerebral artery occlusion/reperfusion (MCAO/R) rat model and an oxygen-glucose deprivation/reoxygenation (OGD/R) model in neuroblastoma cells (SH-SY5Y) were established. The expression of lncRNA-XIST, microRNA-532-5p, and PTEN was detected by qPCR. Binding sites between lncRNA-XIST and microRNA-532-5p, and between microRNA-532-5p and PTEN, were predicted using bioinformatic approaches and validated by dual-luciferase assays. Cell viability, apoptosis, and oxidative stress were assessed by CCK-8 assay, flow cytometry/Western blot, and MDA/SOD assay kits, respectively. LncRNA-XIST and PTEN were highly expressed in the MCAO/R rat brain tissues and the OGD/R cell model, whereas microRNA-532-5p was expressed at low levels. Knockdown of lncRNA-XIST attenuated OGD/R-induced cellular injury. Furthermore, lncRNA-XIST was found to target and inhibit microRNA-532-5p expression, while microRNA-532-5p in turn targeted and suppressed PTEN. The downregulation of PTEN expression and decrease in the apoptotic rate and oxidative stress levels induced by lncRNA-XIST knockdown in the OGD/R cell model were reversed by microRNA-532-5p knockdown. LncRNA-XIST promotes the development and progression of CIRI via the microRNA-532-5p/PTEN axis, offering potential therapeutic targets for CIRI.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0