Advanced biology最新文献_第3页

Loss of BCAP31 or POLR1E Accelerates Aging in C. elegans and is Linked to Senescence in Vascular Endothelium BCAP31或POLR1E的缺失加速了线虫的衰老，并与血管内皮的衰老有关

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.70146

Mengyu Li, Zhenyuan Guo, Yuwei Jiang, Lianghao Zhao, Yuxiang Zhang, Lingxin Meng, Chengzhi Ni, Zhiqiang Chen, Lu Zhang

{"title":"Loss of BCAP31 or POLR1E Accelerates Aging in C. elegans and is Linked to Senescence in Vascular Endothelium","authors":"Mengyu Li, Zhenyuan Guo, Yuwei Jiang, Lianghao Zhao, Yuxiang Zhang, Lingxin Meng, Chengzhi Ni, Zhiqiang Chen, Lu Zhang","doi":"10.1002/adbi.70146","DOIUrl":"https://doi.org/10.1002/adbi.70146","url":null,"abstract":"<div>\u0000 \u0000 <p>Aging involves the progressive decline of cellular function, yet the key molecular regulators that determine aging require further identification. Building on our previous finding that the intracellular domain of CD44 (CD44ICD) is a critical aging regulator, we performed an interactome screen to identify its functional partners. This approach revealed BCAP31 and POLR1E as two previously unrecognized modulators of senescence. Depletion of their respective orthologs in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) shortened lifespan and accelerated age-related functional decline. In human vascular endothelial cells, knockdown of BCAP31 or POLR1E induced premature cellular senescence, whereas their overexpression countered it. Importantly, the protein levels of both BCAP31 and POLR1E decreased during physiological aging across species, including in senescent endothelial cells, aged nematodes, and tissues from old mice, with BCAP31 reduction being particularly pronounced in the cardiovascular system. Our findings establish BCAP31 and POLR1E as evolutionarily conserved components of a CD44-associated network that protects against cellular senescence and organismal aging, highlighting new potential targets for mitigating age-related vascular decline.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of GHSRs Facilitates the Excitability of Dentate Gyrus Granule Cells and Short-Term Spatial Memory via Multiple Ionic and Signaling Mechanisms GHSRs的激活通过多种离子和信号机制促进齿状回颗粒细胞的兴奋性和短期空间记忆

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.70145

Chidiebele S. Oraegbuna, Phani K. Kola, Simon L. Griggs, Saobo Lei

{"title":"Activation of GHSRs Facilitates the Excitability of Dentate Gyrus Granule Cells and Short-Term Spatial Memory via Multiple Ionic and Signaling Mechanisms","authors":"Chidiebele S. Oraegbuna, Phani K. Kola, Simon L. Griggs, Saobo Lei","doi":"10.1002/adbi.70145","DOIUrl":"https://doi.org/10.1002/adbi.70145","url":null,"abstract":"<p>Ghrelin is the endogenous ligand of the ghrelin secretagogue receptors (GHSRs). Although the GHSRs are densely expressed in the dentate gyrus (DG) granule cells (GCs), their roles in the DG GCs remain to be fully understood. We probed the actions of ghrelin on the DG GCs with whole-cell recordings and its effects on short-term spatial memory using the Y-maze spontaneous alternation test. Application of ghrelin excited the DG GCs by depressing the G protein-coupled inwardly rectifying K<sup>+</sup> (GIRK) channels and ATP-sensitive K<sup>+</sup> (K<sub>ATP</sub>) channels, activating the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and a TTX-resistant, persistent Na<sup>+</sup> channel. Adenylyl cyclase and EPAC2 were required, whereas phospholipase C and protein kinase A were not needed for the ghrelin-mediated excitation of DG GCs. Overnight fasting augmented the excitability of the DG GCs via activation of GHSRs, suggesting a role for endogenously released ghrelin. Ghrelin also enhanced synaptic recruitment of action potentials in DG GCs. Microinjection of ghrelin into the DG increased the alternating sequences in the Y maze test via GHSR-mediated elevation of cAMP and EPAC signals. Our results provide an action mode to explain the roles of GHSRs in improving cognitive functions.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of BCAP31 or POLR1E Accelerates Aging in C. elegans and is Linked to Senescence in Vascular Endothelium BCAP31或POLR1E的缺失加速了线虫的衰老，并与血管内皮的衰老有关

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.70146

Mengyu Li, Zhenyuan Guo, Yuwei Jiang, Lianghao Zhao, Yuxiang Zhang, Lingxin Meng, Chengzhi Ni, Zhiqiang Chen, Lu Zhang

{"title":"Loss of BCAP31 or POLR1E Accelerates Aging in C. elegans and is Linked to Senescence in Vascular Endothelium","authors":"Mengyu Li, Zhenyuan Guo, Yuwei Jiang, Lianghao Zhao, Yuxiang Zhang, Lingxin Meng, Chengzhi Ni, Zhiqiang Chen, Lu Zhang","doi":"10.1002/adbi.70146","DOIUrl":"https://doi.org/10.1002/adbi.70146","url":null,"abstract":"<div>\u0000 \u0000 <p>Aging involves the progressive decline of cellular function, yet the key molecular regulators that determine aging require further identification. Building on our previous finding that the intracellular domain of CD44 (CD44ICD) is a critical aging regulator, we performed an interactome screen to identify its functional partners. This approach revealed BCAP31 and POLR1E as two previously unrecognized modulators of senescence. Depletion of their respective orthologs in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) shortened lifespan and accelerated age-related functional decline. In human vascular endothelial cells, knockdown of BCAP31 or POLR1E induced premature cellular senescence, whereas their overexpression countered it. Importantly, the protein levels of both BCAP31 and POLR1E decreased during physiological aging across species, including in senescent endothelial cells, aged nematodes, and tissues from old mice, with BCAP31 reduction being particularly pronounced in the cardiovascular system. Our findings establish BCAP31 and POLR1E as evolutionarily conserved components of a CD44-associated network that protects against cellular senescence and organismal aging, highlighting new potential targets for mitigating age-related vascular decline.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Platform for Fast and Cost-Effective Screening of Immunomodulatory Compounds 一种快速、低成本筛选免疫调节化合物的新平台

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.202500696

Suzana Lj. Popovic, Dejan D. Baskic, Marina M. Mitrovic, Sanja D. Matic, Tijana R. Markovic, Marijana S. Stanojevic Pirkovic, Mirela M. Jevtic, Ivana Z. Lazic, Danijela V. Todorovic

{"title":"A Novel Platform for Fast and Cost-Effective Screening of Immunomodulatory Compounds","authors":"Suzana Lj. Popovic, Dejan D. Baskic, Marina M. Mitrovic, Sanja D. Matic, Tijana R. Markovic, Marijana S. Stanojevic Pirkovic, Mirela M. Jevtic, Ivana Z. Lazic, Danijela V. Todorovic","doi":"10.1002/adbi.202500696","DOIUrl":"https://doi.org/10.1002/adbi.202500696","url":null,"abstract":"<div>\u0000 \u0000 <p>Compounds with immunomodulatory properties are increasingly recognized as valuable adjunctive therapies in pathological conditions associated with immune dysregulation. However, their discovery process is often costly and time-consuming. The aim of this study is to develop a low-cost, simple-to-perform, and reliable method for screening immunomodulatory agents. Human peripheral blood mononuclear cells (PBMNCs) are used as a model system. PBMNCs are isolated by density gradient centrifugation and treated with different immunostimulators and immunosuppressors. By measuring several analytes with a gas analyzer, it is revealed that changes in lactate levels alone can be used as an indicator of immune cell activation/suppression. This finding is further confirmed by measuring the expression of cell surface activation markers and HIF1α using flow cytometry, glucose concentration in PBMNC cultures using a gas analyzer, and levels of TNF-ά and IL-10 by ELISA. The results presented here reveal the simplicity and reliability of this innovative platform for screening immunomodulatory compounds.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA-XIST Competitively Binds to MicroRNA-532-5p to Regulate PTEN Expression, Exacerbating Cerebral Ischemia-Reperfusion Injury LncRNA-XIST竞争性结合MicroRNA-532-5p调控PTEN表达，加重脑缺血-再灌注损伤

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-08-04 DOI: 10.1002/adbi.202500593

Xudong Lu, Luhan Chen, Liqi Qian, Xiaoling Zhang, Yanping Wang, Bo Yu

{"title":"LncRNA-XIST Competitively Binds to MicroRNA-532-5p to Regulate PTEN Expression, Exacerbating Cerebral Ischemia-Reperfusion Injury","authors":"Xudong Lu, Luhan Chen, Liqi Qian, Xiaoling Zhang, Yanping Wang, Bo Yu","doi":"10.1002/adbi.202500593","DOIUrl":"https://doi.org/10.1002/adbi.202500593","url":null,"abstract":"<div>\u0000 \u0000 <p>Cerebral ischemia-reperfusion injury (CIRI) is a severe neurological condition. LncRNA-XIST is closely associated with CIRI; however, its underlying mechanism remains unclear. A middle cerebral artery occlusion/reperfusion (MCAO/R) rat model and an oxygen-glucose deprivation/reoxygenation (OGD/R) model in neuroblastoma cells (SH-SY5Y) were established. The expression of lncRNA-XIST, microRNA-532-5p, and PTEN was detected by qPCR. Binding sites between lncRNA-XIST and microRNA-532-5p, and between microRNA-532-5p and PTEN, were predicted using bioinformatic approaches and validated by dual-luciferase assays. Cell viability, apoptosis, and oxidative stress were assessed by CCK-8 assay, flow cytometry/Western blot, and MDA/SOD assay kits, respectively. LncRNA-XIST and PTEN were highly expressed in the MCAO/R rat brain tissues and the OGD/R cell model, whereas microRNA-532-5p was expressed at low levels. Knockdown of lncRNA-XIST attenuated OGD/R-induced cellular injury. Furthermore, lncRNA-XIST was found to target and inhibit microRNA-532-5p expression, while microRNA-532-5p in turn targeted and suppressed PTEN. The downregulation of PTEN expression and decrease in the apoptotic rate and oxidative stress levels induced by lncRNA-XIST knockdown in the OGD/R cell model were reversed by microRNA-532-5p knockdown. LncRNA-XIST promotes the development and progression of CIRI via the microRNA-532-5p/PTEN axis, offering potential therapeutic targets for CIRI.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Value of Oxidative Stress-Related Features Mined by WGCNA and Machine Learning in Respiratory Syncytial Virus Infection WGCNA与机器学习挖掘氧化应激相关特征对呼吸道合胞病毒感染的诊断价值

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-07-25 DOI: 10.1002/adbi.70142

Ying Lei, Jiahuan He, Weili Lu, Xueqing Ma, Zhiyu Wu, Tao Wang

{"title":"Diagnostic Value of Oxidative Stress-Related Features Mined by WGCNA and Machine Learning in Respiratory Syncytial Virus Infection","authors":"Ying Lei, Jiahuan He, Weili Lu, Xueqing Ma, Zhiyu Wu, Tao Wang","doi":"10.1002/adbi.70142","DOIUrl":"10.1002/adbi.70142","url":null,"abstract":"<div>\u0000 \u0000 <p>Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory tract infections in infants and young children, with insufficient early diagnostic biomarkers and unclear molecular mechanisms. Oxidative stress (OS) is central to RSV-associated immune disorders and tissue damage. GEO datasets (GSE105450, GSE77087) were used to screen differentially expressed genes (DEGs); OS-related DEGs were identified via WGCNA. Four machine learning algorithms screened core diagnostic genes, with in vitro validation (A549/BEAS-2B cells) by qRT-PCR, shRNA-ID3 silencing, immunofluorescence and ELISA. ID3, MATK and ZMAT3 were potential diagnostic biomarkers (training AUC = 0.859, validation AUC = 0.725), upregulated in RSV-infected samples, correlated with immune cell infiltration, and involved in AKT/ERBB pathways. RSV upregulated ID3; ID3 knockdown promoted RSV replication and exacerbated OS. ID3, MATK and ZMAT3 are potential RSV diagnostic biomarkers; ID3 exerts host protection by regulating OS and inhibiting RSV replication, providing new targets for early diagnosis and intervention.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Determining Sirtuin-1 Target Engagement 决定Sirtuin-1目标参与的因素。

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-07-25 DOI: 10.1002/adbi.202500663

Petra Neumann-Staubitz, Yannick Burgdorf, Sarah Hofmann, Heinz Neumann

{"title":"Factors Determining Sirtuin-1 Target Engagement","authors":"Petra Neumann-Staubitz, Yannick Burgdorf, Sarah Hofmann, Heinz Neumann","doi":"10.1002/adbi.202500663","DOIUrl":"10.1002/adbi.202500663","url":null,"abstract":"<p>Sirtuin-1 (Sirt1) is a key NAD<sup>+</sup>-dependent deacylase regulating metabolism, stress responses, genome stability, and aging. Although well-characterized biochemically and structurally, its substrate selectivity remains unclear: in vitro, Sirt1 appears promiscuous, while in vivo it activates particular processes in response to different stimuli. Emerging evidence indicates that selectivity arises from multiple regulatory layers beyond the catalytic site. Here we review how post-translational modifications (PTMs) ‒ including phosphorylation, acetylation, and glycosylation ‒ modulate activity, localization, and substrate affinity. For instance, phosphorylation at S27/T530 (by JNK1) or S682 (by HIPK2) affects nuclear translocation, substrate targeting, or complex formation with cofactors such as AROS and DBC1. Protein–protein interactions, for example with DBC1, PACS2, and transcription factors, further direct Sirt1 to specific substrates or compartments, functioning as allosteric regulators. Spatial compartmentalization, including nucleocytoplasmic shuttling and localization to promyelocytic leukemia nuclear bodies (PML-NBs), integrates Sirt1 into defined signaling contexts. Moreover, liquid–liquid phase separation (LLPS) may concentrate Sirt1 and substrates within condensates, enhancing its selectivity. Overall, Sirt1 specificity emerges from PTMs, interactions, localization, and phase behavior ‒ offering a framework for developing selective modulators in metabolic and age-related diseases.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 20-HETE/GPR75 Axis Drives NLRP1 Inflammasome Activation via p53 in Bronchopulmonary Dysplasia 在支气管肺发育不良中，20-HETE/GPR75轴通过p53驱动NLRP1炎性体激活。

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-07-23 DOI: 10.1002/adbi.202500321

QiuXiang Xu, XiaoLi Tian, Bin Li, Jian Kang

{"title":"The 20-HETE/GPR75 Axis Drives NLRP1 Inflammasome Activation via p53 in Bronchopulmonary Dysplasia","authors":"QiuXiang Xu, XiaoLi Tian, Bin Li, Jian Kang","doi":"10.1002/adbi.202500321","DOIUrl":"10.1002/adbi.202500321","url":null,"abstract":"<div>\u0000 \u0000 <p>Bronchopulmonary dysplasia (BPD), a debilitating chronic respiratory condition afflicting preterm neonates, stems from oxidative injury and is characterized by arrested alveolar growth and aberrant inflammatory responses. This investigation uncovers a novel mechanistic axis in which the 20-hydroxyeicosatetraenoic acid (20-HETE)/GPR75 signaling cascade acts as a previously unrecognized driver of NLRP1 inflammasome activation in neonatal BPD, thereby linking oxidative stress to inflammatory signaling via p53. Using murine hyperoxia models, we observe that excessive oxygen exposure selectively upregulates GPR75 expression and 20-HETE biosynthesis within alveolar epithelial cells. Genetic inactivation of <i>Gpr75</i> in vivo significantly attenuated hyperoxia-induced lung injury, oxidative stress, and NLRP1-dependent secretion of interleukin-1β and interleukin-18. Mechanistic dissection revealed that 20-HETE induces NLRP1 inflammasome assembly in rat alveolar epithelia via p53-mediated signaling. In vitro corroboration via lentiviral-mediated Gpr75 silencing, with scrambled shRNA serving as a negative control, further validated its role in hyperoxia-triggered NLRP1 activation and cytokine release (P < 0.01 vs. scrambled control). Collectively, these findings establish the 20-HETE/GPR75 axis as a critical regulator of inflammasome-dependent pathogenesis in BPD. Blockade of this axis inhibits inflammasome assembly and downstream inflammation by suppressing p53 signaling. These results highlight this axis as a promising therapeutic target to mitigate oxygen-induced injury and inflammatory sequelae in preterm infants.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macroporous Poly-N-Isopropylacrylamide and Cationic Co-Polymer Hydrogels: Long-Term Subcutaneous Implant Biocompatibility 大孔聚n -异丙基丙烯酰胺和阳离子共聚物水凝胶：长期皮下植入生物相容性。

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-07-16 DOI: 10.1002/adbi.202500529

Virginia Capella, Jimena Arri, Ana C. Liaudat, Nahir D. Panozzo Lacunza, Bianca A. Opizzo Balza, Rocio B. Bonino, Gricelda D. Morilla, Matias Caverzan, Cesar A. Barbero, Pablo Bosch, Claudia R. Rivarola, Nancy Rodriguez

{"title":"Macroporous Poly-N-Isopropylacrylamide and Cationic Co-Polymer Hydrogels: Long-Term Subcutaneous Implant Biocompatibility","authors":"Virginia Capella, Jimena Arri, Ana C. Liaudat, Nahir D. Panozzo Lacunza, Bianca A. Opizzo Balza, Rocio B. Bonino, Gricelda D. Morilla, Matias Caverzan, Cesar A. Barbero, Pablo Bosch, Claudia R. Rivarola, Nancy Rodriguez","doi":"10.1002/adbi.202500529","DOIUrl":"10.1002/adbi.202500529","url":null,"abstract":"<div>\u0000 \u0000 <p>Biomaterials have been developed with a great potential in tissue engineering. Hydrogels, especially those based on poly-<i>N</i>-isopropylacrylamide (PNIPAM), have innate mechanical and physicochemical similarities with the native extracellular matrix. We have previously reported the in Vitro compatibility of PNIPAM and its copolymers with a varied number of cell lines, displaying good proliferation rates and bioadhesive capacity. However, the in Vivo biocompatibility assessment is necessary to evaluate. In this work, biocompatibility and immunological acceptance of macroporous hydrogels based on PNIPAM and its co-polymer PNIPAM-co-3%APTA (3-acrylamidopropyl chloride)trimethyl-ammonium) were analyzed using a Wistar female rat model. Hydrogels were synthesized by free radical polymerization and an in Vitro assessment was performed in RAW 264.7 murine macrophages. Then, polymers were implanted subcutaneously and 3-months afterward, grafts were retrieved along with blood and organ samples for haematological, blood chemistry and histological analysis. Cytotoxicity and nitric oxide production did not show variability for the hydrogels. No alterations in the healing process and complete blood count were observed as well as in systemic, liver or kidney toxicity. Histological analysis revealed graft´s integration with the surrounding tissue, without evidence of fibrous capsule or immune cell infiltration. Therefore, these hydrogels are promising biocompatible scaffolds to be used as tissue-engineered constructs.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Context-Dependent Functional Outcomes of Mitochondrial Transfer: A Donor–Recipient Perspective 线粒体移植的环境依赖性功能结果：供体-受体视角。

IF 3.2 3区生物学

Advanced biology Pub Date : 2026-07-16 DOI: 10.1002/adbi.70137

Bin Wang, Tian Lin, Xinying Mo, Benson Peter Mugaka, Guangjie Tai, Ming Xu

{"title":"Context-Dependent Functional Outcomes of Mitochondrial Transfer: A Donor–Recipient Perspective","authors":"Bin Wang, Tian Lin, Xinying Mo, Benson Peter Mugaka, Guangjie Tai, Ming Xu","doi":"10.1002/adbi.70137","DOIUrl":"10.1002/adbi.70137","url":null,"abstract":"<div>\u0000 \u0000 <p>Intercellular mitochondrial transfer has emerged as a fundamental mechanism regulating tissue homeostasis and disease progression, yet its complex regulatory network remains incompletely understood. This review synthesizes the principal transfer mechanisms, focusing on tunneling nanotube (TNT)–mediated direct contact and extracellular vesicle (EV)–mediated indirect transport. Based on the functional disparity between donor and recipient cells, we categorize four prototypical pairing modes and delineate the functional diversity of mitochondrial transfer in metabolic support, tissue repair, and stress responses. Building on this framework, we propose five key determinants as the core dimensions for understanding transfer efficiency and functional outcomes across various contexts: donor mitochondrial quality, donor transfer capacity, recipient metabolic demand, recipient integration capacity, and donor–recipient compatibility. Integrating these factors provides a conceptual basis for explaining the dual effects of mitochondrial transfer—supporting tissue regeneration and functional recovery while, in certain microenvironments, driving pathological reprogramming. This review offers a comprehensive framework for mechanistic studies and clinical translation of mitochondrial transfer and highlights its therapeutic potential in regenerative medicine, aging intervention, and cancer therapy.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0