Advanced biology最新文献_第3页

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles. 巨型聚合物-脂质混合囊泡中微管原位聚合产生的不同网络形态。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-03-12 DOI: 10.1002/adbi.202400601

Paula De Dios Andres, Mousumi Akter, Cecilie Ryberg, Brigitte Städler, Allen P Liu

引用次数: 0

The Aging Substantia Nigra is Characterized by ROS Accumulation Potentially Resulting in Increased Neuroinflammation and Cytoskeletal Remodeling 衰老的黑质下部具有 ROS 积累的特征，可能导致神经炎症和细胞骨骼重塑的增加。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-03-12 DOI: 10.1002/adbi.202400814

Britta Eggers, Simone Steinbach, Isabel Gil Aldea, Sharon Keers, Mariana Molina, Lea T. Grinberg, Helmut Heinsen, Renata E. Paraizo Leite, Johannes Attems, Caroline May, Katrin Marcus

{"title":"The Aging Substantia Nigra is Characterized by ROS Accumulation Potentially Resulting in Increased Neuroinflammation and Cytoskeletal Remodeling","authors":"Britta Eggers, Simone Steinbach, Isabel Gil Aldea, Sharon Keers, Mariana Molina, Lea T. Grinberg, Helmut Heinsen, Renata E. Paraizo Leite, Johannes Attems, Caroline May, Katrin Marcus","doi":"10.1002/adbi.202400814","DOIUrl":"10.1002/adbi.202400814","url":null,"abstract":"Aging is a progressive and irreversible process, serving as the primary risk factor for neurodegenerative disorders. This study aims to identify the molecular mechanisms underlying physiological aging within the substantia nigra, which is primarily affected by Parkinson's disease, and to draw potential conclusions on the earliest events leading to neurodegeneration in this specific brain region. The characterization of essential stages in aging progress can enhance knowledge of the mechanisms that promote the development of Parkinson's disease. To gain a comprehensive overview three study groups are utilized: young individuals (mean age: 28.7 years), middle-aged (mean age: 62.3 years), and elderly individuals (mean age: 83.9 years). Using the proteomic approach, crucial features of physiological aging are able to be identified. These include heightened oxidative stress, enhanced lysosomal degradation, autophagy, remodeling of the cytoskeleton, changes in the structure of the mitochondria, alterations in vesicle transportation, and synaptic plasticity.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-03-04 DOI: 10.1002/adbi.202400717

GaYoung Park, Josh A. Grey, Foteini Mourkioti, Woojin M. Han

{"title":"3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function","authors":"GaYoung Park, Josh A. Grey, Foteini Mourkioti, Woojin M. Han","doi":"10.1002/adbi.202400717","DOIUrl":"10.1002/adbi.202400717","url":null,"abstract":"Muscle stem cells (MuSCs) play a crucial role in skeletal muscle regeneration, residing in a niche that undergoes dimensional and mechanical changes throughout the regeneration process. This study investigates how 3D confinement and stiffness encountered by MuSCs during the later stages of regeneration regulate their function, including stemness, activation, proliferation, and differentiation. An asymmetric 3D hydrogel bilayer platform is engineered with tunable physical constraints to mimic the regenerating MuSC niche. These results demonstrate that increased 3D confinement maintains Pax7 expression, reduces MuSC activation and proliferation, inhibits differentiation, and is associated with smaller nuclear size and decreased H4K16ac levels, suggesting that mechanical confinement modulates both nuclear architecture and epigenetic regulation. MuSCs in unconfined 2D environments exhibit larger nuclei and higher H4K16ac expression compared to those in more confined 3D conditions, leading to progressive activation, expansion, and myogenic commitment. This study highlights the importance of 3D mechanical cues in MuSC fate regulation, with 3D confinement acting as a mechanical brake on myogenic commitment, offering novel insights into the mechano-epigenetic mechanisms that govern MuSC behavior during muscle regeneration.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CS12192 Reverses Alopecia Areata by Selectively Targeting JAK3/JAK1/TBK1.

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-28 DOI: 10.1002/adbi.202400769

Heping Jin, Zongyang Li, Xinwen Li, Qiongqiong Xu, Beizhong Chen, Song Shan, Desi Pan, Peng Hu, Shengjian Huang

{"title":"CS12192 Reverses Alopecia Areata by Selectively Targeting JAK3/JAK1/TBK1.","authors":"Heping Jin, Zongyang Li, Xinwen Li, Qiongqiong Xu, Beizhong Chen, Song Shan, Desi Pan, Peng Hu, Shengjian Huang","doi":"10.1002/adbi.202400769","DOIUrl":"https://doi.org/10.1002/adbi.202400769","url":null,"abstract":"The approval of JAK inhibitors, represented by baricitinib, for the treatment of alopecia areata (AA) has ushered in a new era. However, their excessive immunosuppressive effects have also raised numerous concerns. Therefore, the development of JAK inhibitors with different selectivities may enhance drug safety while maintaining therapeutic efficacy. CS12192, developed by our team, is a selective JAK3 inhibitor that also partially inhibits JAK1 and TBK1. In this study, we evaluate the effectiveness of CS12192 and baricitinib in a C3H/HeJ skin-transplanted AA mouse model and conduct in-depth analysis of the similarities and differences in the mechanisms of the two compounds using mass cytometry (CyTOF) and RNA-seq technology. The results show that CS12192 could reverse hair growth inhibition in AA mice in a dose-dependent manner, with the high-dose group exhibiting comparable effectiveness to baricitinib but with better safety. Further research revealed that both compounds significantly reduced the infiltration of immune cells, particularly CD8+ T cells, in the skin of AA animals. CyTOF and RNA-seq analysis revealed the mechanistic similarities between CS12192 and baricitinib in regulating AA-related immune cells and signaling pathways. In conclusion, CS12192, as a novel selective JAK inhibitor, holds great potential for the treatment of AA.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400769"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Detection of Lipid Accumulation in Cells Using Magnetic Levitation.

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-27 DOI: 10.1002/adbi.202200142

Kazim Kerim Moncal, Laeya Abdoli Najmi, Rakhi Gupta, Malavika Ramarao, Joshua W Knowles, Chong Y Park, Naside Gozde Durmus

{"title":"Label-Free Detection of Lipid Accumulation in Cells Using Magnetic Levitation.","authors":"Kazim Kerim Moncal, Laeya Abdoli Najmi, Rakhi Gupta, Malavika Ramarao, Joshua W Knowles, Chong Y Park, Naside Gozde Durmus","doi":"10.1002/adbi.202200142","DOIUrl":"https://doi.org/10.1002/adbi.202200142","url":null,"abstract":"Dysfunction in adipose tissue can cause serious health problems, including obesity, type-2 diabetes, and cardiovascular disease, significantly reducing human life expectancy. Differences in differentiation and lipid accumulation in adipocytes reflect their functional status, making it important to characterize adipocytes by monitoring biophysical changes during adipogenic differentiation. However, there is currently no specific cell surface marker to separate mature adipocytes from non-adipose cells based on their lipid content, and separation of mature adipocytes is challenging due to handling limitations without fixation, antibody staining, or particle conjugation. Here, we report a biomarker-free, magnetic levitation-based method to detect density changes and quantify the accumulation of lipid-rich droplets within differentiating adipogenic cells. Magnetic levitation revealed density changes within preadipocytes differentiating towards mature adipocytes, with density decreasing over time as cells accumulated lipids. We then used lipid droplets as an intracellular marker to quantify lipid accumulation in single adipocytes during adipogenesis. The significant density changes correlated with cell morphology and lipid droplet morphology within the cytoplasm. For the first time, free-floating lipid vesicle density was measured using magnetic levitation. This unique method enables efficient detection and quantification of dynamically evolving lipid droplets in cells, proving beneficial for modeling lipid storage-related diseases and drug screening applications.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2200142"},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-X-C Motif Ligand 1 Induces Cell Migration by Upregulating ICAM-1 Expression by Activating PI3K/Akt and NF-κB Signaling Pathway in Liver Cancer

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-27 DOI: 10.1002/adbi.202400295

Yi-Hsin Chen, Chih-Chun Chu, Ju-Fang Liu, Hong-Shiee Lai, You-Tzung Chen

{"title":"C-X-C Motif Ligand 1 Induces Cell Migration by Upregulating ICAM-1 Expression by Activating PI3K/Akt and NF-κB Signaling Pathway in Liver Cancer","authors":"Yi-Hsin Chen, Chih-Chun Chu, Ju-Fang Liu, Hong-Shiee Lai, You-Tzung Chen","doi":"10.1002/adbi.202400295","DOIUrl":"10.1002/adbi.202400295","url":null,"abstract":"Human hepatocellular carcinoma (HCC) is the most common liver cancer and the third leading cause of cancer-related deaths worldwide. HCC is a malignant tumor that can lead to intrahepatic and extrahepatic metastases. Intercellular adhesion molecule 1 (ICAM-1) is involved in cancer metastasis. ICAM-1 enhances cell-cell interactions by promoting adhesion and facilitating cell movement within the extracellular matrix. Moreover, ICAM-1 is more abundant in cancerous hepatocytes than in non-cancerous ones. Chemokine (C-X-C motif) ligand 1 (CXCL1) is found in diverse cancers, including melanoma, breast, lung, pancreatic, colorectal, and prostate. Several studies show a correlation between CXCL1 overexpression and poor prognosis in cancer. CXCL1 has been identified as a candidate gene that could function as a clinically relevant biomarker in HCC. However, the role of CXCL1 in cancer metastasis in HCC is poorly delineated. In this study, Gene Expression Omnibus (GEO) database analysis revealed a positive correlation between CXCL1 level and the progression and metastasis of hepatocellular carcinoma patients. CXCL1 treatment facilitates cell movement through inducing ICAM-1 expression. The Phosphoinositide 3-kinase (PI3K)/Akt/Nuclear Factor kappa B (NF-kB) signaling pathway plays a crucial role in CXCL1-regulated ICAM-1 production and cell motility. Thus, CXCL1 represents a promising therapeutic target for treating metastatic hepatocellular carcinoma.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-19 DOI: 10.1002/adbi.202400824

Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Burton Yang, Yu Sun, Lidan You

{"title":"Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone","authors":"Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Burton Yang, Yu Sun, Lidan You","doi":"10.1002/adbi.202400824","DOIUrl":"10.1002/adbi.202400824","url":null,"abstract":"Bone metastasis is a devastating complication for advanced-stage prostate cancer patients. Osteocytes, as the primary mechanosensors in bone, have been recently investigated for their role in prostate cancer bone metastasis. In vivo findings show potential benefits of exercise as a preventative intervention strategy for bone metastasis. In contrast, in vitro studies indicate direct prostate cancer-osteocyte interactions under mechanical loading promote prostate cancer growth and migration. These findings are not consistent with in vivo results and may be more reflective of late-stage metastatic colonization. Here, the role of flow-stimulated osteocytes during early-stage bone metastasis, particularly prostate cancer-endothelial interactions, is examined. Flow-stimulated osteocytes reduce PC-3 prostate cancer cell adhesion and trans-endothelial migration by 32.3% and 40% compared to static controls. Both MLO-Y4 and primary murine osteocytes under mechanical loading regulate the extravasation distance and frequency of PC-3 cells in a microfluidic tissue model. Application of vascular cellular adhesion molecule 1 (VCAM-1) neutralizing antibody abolishes the difference in cancer cell adhesion, extravasation frequency, and number of extravasated PC-3 cells between static and flow-stimulated groups. Taken together, the role of osteocytes in early-stage bone metastasis using PC-3 cells as a model is demonstrated here, bridging the gap between in vitro and in vivo findings.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Naringin and Zinc Treatment on Biochemical, Molecular, and Histological Alterations in Stomach and Pancreatic Tissues of STZ-Induced Diabetic Rats

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400688

Al-Shimaa M. Abas, Mohamed H. Sherif, Sarah Ibrahim

{"title":"Effects of Naringin and Zinc Treatment on Biochemical, Molecular, and Histological Alterations in Stomach and Pancreatic Tissues of STZ-Induced Diabetic Rats","authors":"Al-Shimaa M. Abas, Mohamed H. Sherif, Sarah Ibrahim","doi":"10.1002/adbi.202400688","DOIUrl":"10.1002/adbi.202400688","url":null,"abstract":"Diabetes mellitus is a chronic metabolic disorder that affects multiple organs, including the stomach. This research examines the effects of naringin and/or zinc on stomach and pancreatic tissues of streptozotocin-induced diabetic rats. Type 2 diabetes is induced by intraperitoneal injection of nicotinamide and streptozotocin. Three weeks after diabetes induction, rats receive eight weeks of treatment. Malondialdehyde and total antioxidant capacity are estimated colorimetrically. Asprosin and P-selectin levels are assessed via ELISA. Quantitative RT-PCR analysis of nuclear factor kappa B (NF-кB), peroxisome proliferator-activated receptor gamma (PPAR γ), and nuclear factor erythroid 2-related factor 2 (Nrf-2) genes is carried out. Tumor necrosis factor-alpha (TNF-α) is assessed immunohistochemically, and stomach and pancreatic tissues are examined histologically. Combined naringin and zinc treatment significantly reduces gastric Malondialdehyde, serum asprosin, and P-selectin levels in serum, stomach, and pancreas compared to diabetic rats. Additionally, gastric NF-кB expression is significantly lower, while PPAR γ and Nrf-2 expressions are significantly higher compared to diabetic rats. Immunohistochemical analysis and histopathological examination confirm these findings. In conclusion, combined naringin and zinc treatment significantly improves gastric alterations in diabetic rats by reducing oxidative stress and inflammation. Nonetheless, it shows no additional impacts on pancreatic tissue compared to naringin or zinc alone.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400571

Minju Kim, Kanghoon Choi, Amy Lin, Jungkyu Kim

{"title":"Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.","authors":"Minju Kim, Kanghoon Choi, Amy Lin, Jungkyu Kim","doi":"10.1002/adbi.202400571","DOIUrl":"https://doi.org/10.1002/adbi.202400571","url":null,"abstract":"Corneal blindness remains a significant global health challenge, with limited treatment options due to donor tissue scarcity outside of the United States and inadequate in vitro models. This review analyzes the current state of cornea chip technology, addressing fundamental challenges and exploring future directions. Recent advancements in biomaterials and fabrication techniques are discussed that aim to recapitulate the complex structure and function of the human cornea, including the multilayered epithelium, organized stroma, and functional endothelium. The review highlights the potential of the cornea chips to revolutionize ocular research by offering more predictive and physiologically relevant models for drug screening, disease modeling, and personalized medicine. Current designs, their applications in studying drug permeability, barrier function, and wound healing, and their limitations in replicating native corneal architecture, are examined. Key challenges include integrating corneal curvature, basement membrane formation, and innervation. Applications are explored in modeling diseases like keratitis, dry eye disease, keratoconus, and Fuchs' endothelial dystrophy. Future directions include incorporating corneal curvature using hydraulically controlled systems, using patient-derived cells, and developing comprehensive disease models to accelerate therapy development and reduce reliance on animal testing.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400571"},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome Size Is Positively Correlated with Lifespan in Mammals but Negatively Correlated with Lifespan in Birds

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400633

Juliano Morimoto, Zuzanna Pietras

引用次数: 0