Advanced biology最新文献_第3页

Inhibiting DNA Sensing Pathway Controls Steroid Hyporesponsive Lung Inflammation. 抑制DNA传感通路可控制类固醇低反应性肺部炎症

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-27 DOI: 10.1002/adbi.202400230

Bushra Mdkhana, Narjes Saheb Sharif-Askari, Roberta Cagliani, Baraa Khalid Saleh Al-Sheakly, Rakhee K Ramakrishnan, Fatemeh Saheb Sharif-Askari, Ibrahim Yaseen Hachim, Qutayba Hamid, Mutasem Rawas-Qalaji, Rabih Halwani

{"title":"Inhibiting DNA Sensing Pathway Controls Steroid Hyporesponsive Lung Inflammation.","authors":"Bushra Mdkhana, Narjes Saheb Sharif-Askari, Roberta Cagliani, Baraa Khalid Saleh Al-Sheakly, Rakhee K Ramakrishnan, Fatemeh Saheb Sharif-Askari, Ibrahim Yaseen Hachim, Qutayba Hamid, Mutasem Rawas-Qalaji, Rabih Halwani","doi":"10.1002/adbi.202400230","DOIUrl":"https://doi.org/10.1002/adbi.202400230","url":null,"abstract":"DNA damage underlies the progression of asthma toward a severe, steroid hyporesponsive phenotype. The accumulation of double-stranded DNA within the cytosol triggers the activation of cytosolic DNA-sensing pathways, notably the Stimulator of Interferon Genes (STING) pathway. However, the precise role of STING in driving steroid hyporesponsiveness remains elusive and warrants further investigation. This study evaluates STING levels in human bronchial fibroblasts from severe asthmatic patients and in lung homogenates from a steroid hyporesponsive lung inflammation mouse model. STING level is assessed at baseline, post house dust mites (HDM) stimulation, and following treatment with dexamethasone and STING inhibitor. The effect of STING inhibitors on regulating steroid hyporesponsiveness particularly glucocorticoid receptor (GR)-α/GR-β ratio is also examined. Severe asthmatic fibroblasts exhibit elevated STING/IFN-I pathway activation, further heightened by HDM and a similar pattern is seen in lung homogenates from steroid hyporesponsive mice. Dexamethasone combined with an STING inhibitor reduces STING activity, while dexamethasone alone is ineffective. Interestingly, the STING inhibitor restores steroid sensitivity by increasing the GRα/GRβ ratio. Furthermore, nanoparticle-encapsulated STING inhibitor more effectively reduces airway hyperresponsiveness and restores steroid sensitivity than the free inhibitor. These findings emphasize STING's role in severe asthma pathogenesis, proposing nanoparticle delivery of STING inhibitors as a promising therapeutic strategy.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400230"},"PeriodicalIF":3.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of 7-Day Cold Water Acclimation on Autophagic and Apoptotic Responses in Young Males. 7 天冷水驯化对年轻男性自噬和凋亡反应的影响

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-27 DOI: 10.1002/adbi.202400111

Kelli E King, James J McCormick, Glen P Kenny

{"title":"The Effect of 7-Day Cold Water Acclimation on Autophagic and Apoptotic Responses in Young Males.","authors":"Kelli E King, James J McCormick, Glen P Kenny","doi":"10.1002/adbi.202400111","DOIUrl":"https://doi.org/10.1002/adbi.202400111","url":null,"abstract":"While cold acclimation can enhance thermoregulation in humans, the potential to improve cellular cold tolerance remains unknown. Thus, this work aims to evaluate the effect of a 7-day cold-water acclimation on the cytoprotective mechanism of autophagy in young males. Further, this work assesses changes in cellular cold tolerance by employing hypothermic ex vivo (whole blood) cooling prior to and following acclimation. Peripheral blood mononuclear cells are isolated before and after cold exposures on days 1, 4, and 7 of acclimation and following ex vivo cooling. Proteins associated with autophagy, apoptosis, the heat shock response, and inflammation are analyzed via Western blotting. Indicators of autophagic dysfunction paired with increased apoptotic signaling are prevalent at the beginning of acclimation. At the end of acclimation, autophagic activity increased while apoptotic and inflammatory signaling decreased. Although an elevated heat shock response is observed following cold exposure, this does not change throughout the acclimation. Further, improvements of autophagic activity are observed during ex vivo cooling along with a reduction of apoptotic signaling, albeit still elevated compared to basal levels. This work shows that 7-day cold acclimation elicits improvements in cellular cold tolerance in young males through enhanced autophagic responses concomitant with reductions in apoptotic signaling.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400111"},"PeriodicalIF":3.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Action of Tongjiang Mixture for Treating Reflux Esophagitis: A Study Using Serum Pharmacochemistry and Network Pharmacology. 通江合剂治疗反流性食管炎的作用机制：血清药物化学和网络药理学研究。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-27 DOI: 10.1002/adbi.202400187

Yang Lu, Huang Yuzhen, Gu Yi, Wu Lili, Wang Yan, Tao Weiwei, Liu Wanli

{"title":"Mechanism of Action of Tongjiang Mixture for Treating Reflux Esophagitis: A Study Using Serum Pharmacochemistry and Network Pharmacology.","authors":"Yang Lu, Huang Yuzhen, Gu Yi, Wu Lili, Wang Yan, Tao Weiwei, Liu Wanli","doi":"10.1002/adbi.202400187","DOIUrl":"https://doi.org/10.1002/adbi.202400187","url":null,"abstract":"Tongjiang Mixture (TJM) is a traditional Chinese formula for treating reflux esophagitis (RE). Nevertheless, its active ingredients and potential pharmacological mechanisms are not yet clearly elucidated. This study will identify the active ingredients of TJM using serum pharmacochemistry and to elucidate the mechanism on RE through network pharmacology. The blood-borne ingredients of TJM are identified by the Ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometer. Subsequently, a \"compound-target-disease\" network is established and obtained core targets associated with TJM and RE. Then, the potential signaling pathways are forecasted through the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Finally, the rat model of RE is established to verify the results predicted by network pharmacology through animal experiments. Fifteen blood-borne ingredients of TJM are identified, with eight active ingredients-namely Tangeretin, Tricin, Palmati, Berberine, Limonin, Evodiamine, Tetrahydropalmatine and Rutecarpine - making significant contributions to its efficacy. Moreover, TJM is predicted to act on 193 targets related to RE, involving AKT1, HSP90AA1, PIK3CA, and other targets, which enriches mainly in PI3K/AKT /NF-κB signaling. Additionally, TJM can alleviate inflammation of the esophageal mucosa, reduce pathological damage, and increase gastric pH. It can downregulate PI3K, AKT, and NF-κB mRNA transcription levels and reduce the protein expression of PI3K, AKT, and NF-κB. Furthermore, it can inhibit the overproduction of IL-6, TNF-α and IL-17. TJM can alleviate immune-inflammatory responses and ameliorate RE by restraining the PI3K/AKT pathway and its downstream NF-κB.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400187"},"PeriodicalIF":3.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Method for Fabricating Tissue-Specific Extracellular Matrix Blocks From Decellularized Tissue Powders. 利用脱细胞组织粉末制造组织特异性细胞外基质块的方法。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-27 DOI: 10.1002/adbi.202400398

Jun Negishi, Ayana Yamaguchi, Dan Tanaka, Yoshihide Hashimoto, Yongwei Zhang, Seiichi Funamoto

{"title":"A Method for Fabricating Tissue-Specific Extracellular Matrix Blocks From Decellularized Tissue Powders.","authors":"Jun Negishi, Ayana Yamaguchi, Dan Tanaka, Yoshihide Hashimoto, Yongwei Zhang, Seiichi Funamoto","doi":"10.1002/adbi.202400398","DOIUrl":"https://doi.org/10.1002/adbi.202400398","url":null,"abstract":"Decellularized tissues retain the extracellular matrix (ECM), shape, and composition that are unique to the source tissue. Previous studies using decellularized tissue lysates and powders have shown that tissue-specific ECM plays a key role in cellular function and wound healing. However, creating decellularized tissues composed of tissue-specific ECM with customizable shapes and structures for use as scaffolding materials remains challenging. In this study, a method for compacting decellularized tissue powder into blocks is developed using cold isostatic pressing (CIP). Custom-shaped ECM blocks and composite ECM blocks are fabricated using silicone molds. Additionally, an ECM block with a two-layer structure is obtained through a two-step CIP process. Cells are observed to infiltrate porous ECM blocks that are created using sodium chloride and transglutaminase. These results highlight the development of an effective method for producing ECM blocks using CIP with customizable shapes, compositions, and structures, making them suitable for use as cell culture scaffolds.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400398"},"PeriodicalIF":3.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced 2D Nanomaterials for Phototheranostics of Breast Cancer: A Paradigm Shift. 用于乳腺癌光热疗法的先进二维纳米材料：范式转变。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-14 DOI: 10.1002/adbi.202400441

Arpana Parihar, Kritika Gaur, Paromita Sarbadhikary

{"title":"Advanced 2D Nanomaterials for Phototheranostics of Breast Cancer: A Paradigm Shift.","authors":"Arpana Parihar, Kritika Gaur, Paromita Sarbadhikary","doi":"10.1002/adbi.202400441","DOIUrl":"https://doi.org/10.1002/adbi.202400441","url":null,"abstract":"Breast cancer is the leading cause of women's deaths and associated comorbidities. The advanced and targeted strategies against breast cancer have gained considerable attention due to their potential enhanced therapeutic efficacy over conventional therapies. In this context, phototherapies like photodynamic therapy (PDT) and photothermal therapy (PTT) have shown promise as an effective and alternative strategy due to reduced side effects, noninvasiveness, and spatiotemporal specificity. With the advent of nanotechnology, several types of nanomaterials that have shown excellent prospects in increasing the efficacy of photo therapies have been exploited in cancer treatment. In recent years, 2D nanomaterials have stood out promising because of their unique ultrathin planar structure, chemical, physical, tunable characteristics, and corresponding remarkable physiochemical/biological properties. In this review, the potential and the current status of several types of 2D nanomaterials such as graphene-based nanomaterials, Mxenes, Black phosphorous, and Transition Metal Dichalcogenides for photo/thermo and combination-based imaging and therapy of breast cancer have been discussed. The current challenges and prospects in terms of translational potential in future clinical oncology are highlighted.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400441"},"PeriodicalIF":3.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel UBM/SIS Composite Biological Scaffold for 2-Year Abdominal Defect Repairing and Strength Recovery in Canine Model. 一种新型 UBM/SIS 复合生物支架，用于犬模型腹部缺损修复和力量恢复两年。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-14 DOI: 10.1002/adbi.202400131

Weidong Zhong, Jinshui Chen, Qifeng Xie, Wenyue Cheng, Meibiao Zhao, Yang Sun, Jing Dai, Jian Zhang

{"title":"A Novel UBM/SIS Composite Biological Scaffold for 2-Year Abdominal Defect Repairing and Strength Recovery in Canine Model.","authors":"Weidong Zhong, Jinshui Chen, Qifeng Xie, Wenyue Cheng, Meibiao Zhao, Yang Sun, Jing Dai, Jian Zhang","doi":"10.1002/adbi.202400131","DOIUrl":"https://doi.org/10.1002/adbi.202400131","url":null,"abstract":"Biological scaffolds are widely utilized in hernia treatment due to their exceptional pro-regenerative properties, which mitigate scar formation. However, serious complications occurred, caused by inflammatory response, premature degradation, and mechanical failure. Consequently, improvements of the biological scaffold are necessary to mitigate these risks. In this study, a novel biological scaffold integrating basement membrane-containing urinary bladder matrix (UBM) and small intestinal submucosa (SIS) is developed, and its safety and effectiveness are assessed in comparison to a commercial SIS (c-SIS) scaffold. The introduction of UBM as top surface layers significantly promotes cell adhesion, facilitating rapid formation of isolated regeneration zone. Proteomic analysis has demonstrated a more efficient decellularization of the UBM/SIS scaffold, which subsequently mitigates inflammation in murine models, and promotes the polarization of macrophages toward the pro-healing M2 phenotype in a rat model of abdominal wall muscle defect. Furthermore, a two-year repair trial is conducted on a full-thickness abdominal wall muscle defect in canine model and confirmed that the UBM/SIS scaffold exhibits reduced seroma occurrences and enhanced tissue repair performances. Overall, the efficacy of this novel biological scaffold suggests its potential to minimize hernia recurrence in clinical practice and mitigate patient suffering from severe inflammatory responses.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400131"},"PeriodicalIF":3.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate Identification of Cancer Cells in Complex Pre-Clinical Models Using a Deep-Learning Neural Network: A Transfection-Free Approach (Adv. Biology 11/2024) 利用深度学习神经网络准确识别复杂临床前模型中的癌细胞：无转染方法（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470112

Marilisa Cortesi, Dongli Liu, Elyse Powell, Ellen Barlow, Kristina Warton, Caroline E. Ford

引用次数: 0

Masthead: (Adv. Biology 11/2024) 刊头：（Adv. Biology 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470113

引用次数: 0

Predicting Clinical Outcomes of SARS-CoV-2 Drug Efficacy with a High-Throughput Human Airway Microphysiological System (Adv. Biology 11/2024) 利用高通量人体气道微生理系统预测SARS-CoV-2药物疗效的临床结果（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470111

Landys Lopez Quezada, Felix Mba Medie, Rebeccah J. Luu, Robert B. Gaibler, Elizabeth P. Gabriel, Logan D. Rubio, Thomas J. Mulhern, Elizabeth E. Marr, Jeffrey T. Borenstein, Christine R. Fisher, Ashley L. Gard

引用次数: 0

Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging. 通过数字全息显微镜和定量相位成像对黑色素细胞和 SK-MEL-28 黑色素瘤细胞进行形态学和光学分析。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-11 DOI: 10.1002/adbi.202400346

Ayah A Farhat, Yazan A Almahdi, Fatima Z Alshuhani, Besa Xhabija

{"title":"Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging.","authors":"Ayah A Farhat, Yazan A Almahdi, Fatima Z Alshuhani, Besa Xhabija","doi":"10.1002/adbi.202400346","DOIUrl":"https://doi.org/10.1002/adbi.202400346","url":null,"abstract":"Melanoma, which originates from pigment-producing melanocytes, is an aggressive and deadly skin cancer. Despite extensive research, its mechanisms of progression and metastasis remain unclear. This study uses quantitative phase imaging via digital holographic microscopy, Principal Component Analysis (PCA), and t-distributed Stochastic Neighbor Embedding (t-SNE) to identify the morphological, optical, and behavioral differences between normal melanocytes and SK-MEL-28 melanoma cells. Our findings reveal significant differences in cell shape, size, and internal organization, with SK-MEL-28 cells displaying greater size variability, more polygonal shapes, and higher optical thickness. Phase shift parameters and surface roughness analyses underscore melanoma cells' uniform and predictable textures. Violin plots highlight the dynamic and varied migration of SK-MEL-28 cells, contrasting with the localized movement of melanocytes. Hierarchical clustering of correlation matrices provides further insights into complex morphological and optical relationships. Integrating label-free imaging with robust analytical methods enhances understanding of melanoma's aggressive behavior, supporting targeted therapies and highlighting potential biomarkers for precise melanoma diagnostics and treatment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400346"},"PeriodicalIF":3.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0