Advanced biology最新文献_第3页

Sub-Neuronal Network Profiling of Extracellular Vesicle Release Using a Compartmentalized Neurofluidic Platform 使用区隔化神经流体平台的细胞外囊泡释放的亚神经元网络分析。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500381

Zeynep Malkoc, Esther Stopps, Prince M. K. Asamoah, Stephanie E. McCalla, Anja Kunze

{"title":"Sub-Neuronal Network Profiling of Extracellular Vesicle Release Using a Compartmentalized Neurofluidic Platform","authors":"Zeynep Malkoc, Esther Stopps, Prince M. K. Asamoah, Stephanie E. McCalla, Anja Kunze","doi":"10.1002/adbi.202500381","DOIUrl":"10.1002/adbi.202500381","url":null,"abstract":"<p>Extracellular vesicles (EVs) are membrane-bound vesicles that are secreted by a wide range of organisms and cells, carrying cell-specific receptors and molecular cargo such as proteins and nucleic acids. EVs have emerged as promising biomarkers for cancer and neurodegenerative disorders like Alzheimer's Disease (AD). Traditional methods for isolating neuron-derived EVs from bodily fluids or conditioned media are based on bulk analysis methods, such as ultracentrifugation, isolation reagents, and immunoaffinity-based techniques, and lack spatial resolution to capture localized secretion dynamics. Here, our neurofluidic platform compartmentalizes neuronal networks and enables spatially resolved analysis of EV profiling before subsequent traditional isolation and content screening. This intermediate resolution provides critical insights into localized sub-neuronal EV secretion dynamics in cortical, hippocampal, and brainstem neurons. Using our platform, the influence of growth environment, cell maturation time, and exogenous stressors such as shear and biochemical stress can be unraveled. Biochemical stress is induced through okadaic acid (OA), a PP1A/PP2A inhibitor, which leads to hyperphosphorylation of proteins. In parallel, microRNA expression profiles are shown after OA treatment in primary neuron cultures, indicating an additional transcriptional response. These findings reveal regional differences in EV secretion dynamics associated with neuronal development and external stressors, including shear forces and PP1A/PP2A inhibition.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12914630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Common Immune Responses Differences from Transcriptomic Profiling in Cardiomyocytes upon Coxsackievirus B5 and Echovirus 6 Infection 柯萨奇病毒B5和埃可病毒6感染心肌细胞转录组学分析的共同免疫反应差异

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500281

Yi Xu, Xianwu Lan, Jianwei Chen, Jieyu You, Hiu Tung Ko, Chuqun Fang, Shanshan Cui, Muhammad Adnan Shereen, Zhen Luo, Shaorong Wu

{"title":"The Common Immune Responses Differences from Transcriptomic Profiling in Cardiomyocytes upon Coxsackievirus B5 and Echovirus 6 Infection","authors":"Yi Xu, Xianwu Lan, Jianwei Chen, Jieyu You, Hiu Tung Ko, Chuqun Fang, Shanshan Cui, Muhammad Adnan Shereen, Zhen Luo, Shaorong Wu","doi":"10.1002/adbi.202500281","DOIUrl":"10.1002/adbi.202500281","url":null,"abstract":"<div>\u0000 \u0000 <p>Viral myocarditis (VMC) is a myocardial injury syndrome caused by enterovirus infections, including Coxsackievirus B5 (CVB5) and Echovirus 6 (ECHO6). However, the exact pathogenesis of VMC by enteroviruses remains unclear. Here, the host immune response differences in transcriptomics in human cardiomyocyte AC16 cells upon CVB5 and ECHO6 infections were explored. CVB5 and ECHO6 effectively infected AC16 cells, showing significant viral replication and cytopathic effects at 48 h post-infection. Transcriptomic analysis indicated that both CVB5 and ECHO6 infection induced a series of immune- and inflammation- related genes, including IL6, CCL3, and IFNL1, which were validated by qPCR. Additionally, Ribavirin demonstrated a certain inhibitory effect on the viral replication of both CVB5 and ECHO6 at a concentration of 50 µ<span>m</span>. This study established a systematic comparison of the common transcriptomic differences and immune response characteristics of both CVB5 and ECHO6 infection in human cardiomyocytes. The marked upregulation of immune-related genes suggests that innate immunity and the inflammatory response play critical roles in cardiomyocytes defense against enterovirus infection. Ribavirin showed notable inhibitory activity against both CVB5 and ECHO6. The study sheds light on new insights into the foundations for the pathogenesis of enterovirus-associated VMC and the development of promising therapeutic approaches against VMC.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of LncRNA Dlx6os1 Alleviated High Glucose-Induced Oxidative Stress Mediated by Parkin in Cardiomyocytes LncRNA Dlx6os1抑制可缓解Parkin介导的高糖诱导的心肌细胞氧化应激。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-18 DOI: 10.1002/adbi.202500692

Jiayu Diao, Qiling Gou, Linting Zhang, Fengchao Wu, Lei Liang

{"title":"Suppression of LncRNA Dlx6os1 Alleviated High Glucose-Induced Oxidative Stress Mediated by Parkin in Cardiomyocytes","authors":"Jiayu Diao, Qiling Gou, Linting Zhang, Fengchao Wu, Lei Liang","doi":"10.1002/adbi.202500692","DOIUrl":"10.1002/adbi.202500692","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the effect of lncDlx6os1 on high glucose (HG)-induced oxidative stress and the involvement of the parkin pathway. Methods: Short hairpin RNA of lncDlx6-os1 (sh-lncDlx6os1) and small interfering RNA of Parkin (si-Parkin) were used to inhibit the expression of lncDlx6-os1 and Parkin in cardiomyocytes, respectively. qRT-PCR, western blotting, and flow cytometry were performed to investigate the underlying mechanism. Results: sh-lncDlx6os1 inhibited HG-induced reactive oxygen species (ROS) generation and increased the expression of HO-1 and NRF2 in HG-treated cardiomyocytes. sh-lncDlx6os1 significantly suppressed the expression of the apoptotic molecule caspase3, and increased the expression of the anti-apoptotic molecule Bcl-2, thereby inhibiting HG-induced apoptosis. Meanwhile, sh-lncDlx6os1 increased the expression of Sirt1, PINK1, Parkin, and LC3II/LC3I in HG-treated cardiomyocytes. Suppression of Parkin by si-Parkin weakened the regulatory effect of sh-lncDlx6os1 on apoptosis and caspase3 and Bcl-2 expression. si-Parkin also attenuated the inhibitory effect of sh-lncDlx6os1 on ROS generation and increased HO-1 and NRF2 expressions in HG-treated cardiomyocytes. Conclusion: Our study revealed that the suppression of lncDlx6os1 significantly alleviated HG-induced oxidative stress, which was partially mediated by parkin in cardiomyocytes.</p>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Personalized Models of Biological Barriers and Their Diseases: Recent Progress with Organs-On-Chips 生物屏障及其疾病的个性化模型：器官芯片的最新进展

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-02-11 DOI: 10.1002/adbi.202500536

Franziska Buck, Jeroen Bugter, Gizem Yorukoglu, Mina Kazemzadeh Dastjerd, Thomas E. Winkler

{"title":"Personalized Models of Biological Barriers and Their Diseases: Recent Progress with Organs-On-Chips","authors":"Franziska Buck, Jeroen Bugter, Gizem Yorukoglu, Mina Kazemzadeh Dastjerd, Thomas E. Winkler","doi":"10.1002/adbi.202500536","DOIUrl":"10.1002/adbi.202500536","url":null,"abstract":"<p>Barrier tissues—epithelial and endothelial interfaces that compartmentalize the human body—govern molecular exchange, immune surveillance, and organ homeostasis. Their dysfunction is central to disorders ranging from dermatitis to neurodegeneration. Conventional static cultures fail to capture the relevant microenvironment and typically rely on cell lines that overlook patient-specific genetics. Organs-on-chips (OoCs), by contrast, can recapitulate barrier-specific flow, biomechanics, chemical gradients, and a multicellular architecture. Additionally, incorporating primary or induced pluripotent stem cell (iPSC)-derived cells into OoCs can open new avenues for precision medicine. This review surveys the architectural diversity and physiological functions of human barrier systems and explores how OoC platforms—especially those using patient-derived cells—are advancing barrier disease modeling. It reveals similar core features but also unique barrier characteristics requiring specific adaptations, resulting in varied progress across systems, and continued refinement of iPSC differentiation protocols and OoC engineering is needed overall. Nevertheless, existing biological and technological advances already offer substantial, untapped opportunities to create physiologically relevant, patient-specific disease models and drug-testing platforms, bridging the gap between fundamental biology and translational medicine.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146154890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in Research on Medication-Related Osteonecrosis of the Jaw: From Pathophysiological Mechanisms to Clinical Treatment 颌骨药物相关性骨坏死的研究进展：从病理生理机制到临床治疗。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-28 DOI: 10.1002/adbi.202500627

Xinliang Duan, Ziyin Zhang, Sichen Han, Yao Yuan, Junzi Mi, Yixuan Dai, Yifu Bian, Zilin Wang

引用次数: 0

Cryopreservation of Anopheles gambiae Larvae 冈比亚按蚊幼虫的低温保存。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-27 DOI: 10.1002/adbi.202500633

Purva Joshi, Zachary Chau, Abby Callahan-Muller, Vincent O. Nyasembe, Kala Acha, Ellen M. Dotson, Mehmet Toner, Rebecca D. Sandlin

{"title":"Cryopreservation of Anopheles gambiae Larvae","authors":"Purva Joshi, Zachary Chau, Abby Callahan-Muller, Vincent O. Nyasembe, Kala Acha, Ellen M. Dotson, Mehmet Toner, Rebecca D. Sandlin","doi":"10.1002/adbi.202500633","DOIUrl":"10.1002/adbi.202500633","url":null,"abstract":"<p>Mosquitoes remain a significant global health concern due to their role in transmitting deadly diseases including malaria, dengue, and yellow fever. Recent advances in genetic modification technologies have enabled researchers to better study disease transmission and is an emerging approach for vector control. However, a major bottleneck is the need for continuous rearing of each new strain, an intensive process requiring substantial manual effort and space with inherent risks of cross-contamination, genetic drift, and catastrophic loss. Cryopreservation offers a potential solution, where strains could be stored indefinitely and revived on an as-needed basis. Here, we report a cryopreservation protocol that results in the recovery of viable <i>Anopheles gambiae</i> larvae. After thawing, 82% of larvae showed signs of viability, including wriggling and mouth brush movements. Approximately 15% further exhibited coordinated swimming behavior. Although post-thaw survival was limited to 24 h, this study provides the first evidence that mosquito larvae can survive cryopreservation, representing a key milestone toward long-term storage of mosquito lines.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

mmu_circ_0000217 Promotes Osteogenic Differentiation in OCCM-30 Cells via the miR-3064-3p/DKK1 Axis mmu_circ_0000217通过miR-3064-3p/DKK1轴促进OCCM-30细胞的成骨分化。

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-27 DOI: 10.1002/adbi.202500581

Kegang Wang, Cong Zhang, Yu Mai, Yan Zhang, Ying Li, Chong Li

{"title":"mmu_circ_0000217 Promotes Osteogenic Differentiation in OCCM-30 Cells via the miR-3064-3p/DKK1 Axis","authors":"Kegang Wang, Cong Zhang, Yu Mai, Yan Zhang, Ying Li, Chong Li","doi":"10.1002/adbi.202500581","DOIUrl":"10.1002/adbi.202500581","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Periodontitis leads to irreversible periodontal tissue damage, and current treatments lack sufficient regenerative capacity. This study investigated the role of mmu_circ_0000217 in osteogenic differentiation of OCCM-30 cementoblastic cells and its underlying mechanism, aiming to provide a theoretical basis for periodontal tissue regeneration. This study first identified the upregulated expression of mmu_circ_0000217 during osteogenic differentiation of OCCM-30 cells using high-throughput sequencing. The impact of mmu_circ_0000217 on cell proliferation and apoptosis was evaluated in OCCM-30 cells by modulating its expression and using CCK-8 assays and TUNEL staining. Morphological changes related to mineralization and differentiation were examined using Alkaline Phosphatase (ALP) and Alizarin Red S staining (ARS). Osteogenic gene and protein expressions were analyzed with QPCR and Western blotting, which also detected JAK-STAT3 signaling pathway activation. High-throughput sequencing identified mmu_circ_0000217 as the most significantly upregulated circRNA during osteogenic induction. Functional experiments demonstrated that mmu_circ_0000217 overexpression significantly enhanced cell proliferation, inhibited apoptosis, and potentiated mineralization, as evidenced by increased ALP activity and Alizarin Red S staining. Conversely, its knockdown produced the opposite effects. Mechanistically, mmu_circ_0000217 functioned as a molecular sponge for miR-3064-3p, which led to the derepression of its target, DKK1, and consequent activation of the JAK-STAT3 signaling pathway. Mmu_circ_0000217 activated the JAK-STAT3 signaling pathway by adsorbing miR-3064-3p, promoted cell proliferation, inhibited apoptosis, and enhanced osteoblast differentiation. These findings enhance our understanding of the molecular mechanisms behind periodontal tissue regeneration.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-20 DOI: 10.1002/adbi.202500710

Zhengyi Yan, Luowei Yuan, Jinxing Wang, Shen Gu, Yong Lei

{"title":"PRC2-Related Epigenetic Age Acceleration in Acute Myeloid Leukemia with DNMT3A and IDH2 Mutations","authors":"Zhengyi Yan, Luowei Yuan, Jinxing Wang, Shen Gu, Yong Lei","doi":"10.1002/adbi.202500710","DOIUrl":"10.1002/adbi.202500710","url":null,"abstract":"<p>Aging is closely linked to epigenetic remodeling, with DNA methylation (DNAm) emerging as a robust biomarker for estimating epigenetic age (EA) and quantifying senescence. Dysregulation of aging-associated DNAm has been implicated in diverse pathologies, including acute myeloid leukemia (AML). However, the effect of these epigenetic alterations in diseases and the underlying mechanism remains largely uncharacterized. Using causality-enriched epigenetic clocks, we identified that adaptive DNAm dynamics are sensitive to short-term therapeutic intervention in treating AML and may exhibit adaptive effects linked to better health outcomes. Subsequently, integrative genomic analysis showed significant associations between epigenetic aging and recurrent AML driver mutated genes, particularly <i>DNMT3A</i> and <i>IDH2</i>. The elevated adaptive aging associates with improved overall survival in cytogenetically normal AML harboring these mutations, highlighting its prognostic value in specific genomic contexts. Mechanistic analysis demonstrated that differentially methylated CpG sites in mutated gene-specific AML subtypes are enriched at polycomb repressive complex 2 (PRC2) targets. These findings link mutation-specific epigenetic aging, PRC2-mediated methylation dynamics, and AML pathogenesis, offering insights into how aging-related epigenetic dysregulation fosters malignant transformation. This study shows that AdaptAge can help reveal AML‑related DNAm dynamics when combined with genetic stratification, suggesting a path toward future biomarker development.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12817234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Roles of FAHD-1 and PYC-1 in Mitochondrial Function, Behavior, and Longevity in C. elegans FAHD-1和PYC-1在线虫线粒体功能、行为和寿命中的协同作用

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-20 Epub Date: 2025-12-22 DOI: 10.1002/adbi.202500410

Riccardo Giaquinta, Andreas Andric, Matthias Scheppach, Alexander K. H. Weiss

{"title":"Synergistic Roles of FAHD-1 and PYC-1 in Mitochondrial Function, Behavior, and Longevity in C. elegans","authors":"Riccardo Giaquinta, Andreas Andric, Matthias Scheppach, Alexander K. H. Weiss","doi":"10.1002/adbi.202500410","DOIUrl":"10.1002/adbi.202500410","url":null,"abstract":"<p>Mitochondrial metabolism plays a central role in organismal physiology and aging. In <i>Caenorhabditis elegans</i>, FAHD-1 (oxaloacetate decarboxylase) and PYC-1 (pyruvate carboxylase) catalyze opposing reactions that influence oxaloacetate homeostasis within the tricarboxylic acid cycle. To dissect their functional interplay, we analyzed single- and double-knockout strains generated by CRISPR/Cas9 alongside the classical allele. <i>Fahd-1</i> mutants exhibit impaired mitochondrial respiration, reduced motility, and early egg-laying onset, whereas <i>pyc-1</i> mutants display increased locomotion and enhanced metabolic flexibility. Paradoxically, although each single mutantion extended lifespan, combining them restored wild-type lifespan and partially normalized respiratory function, suggesting a compensatory interaction. These findings establish FAHD-1 and PYC-1 as antagonistic mitochondrial enzymes whose balance governs locomotion, reproduction, and lifespan in <i>C. elegans</i>, providing a conceptual framework for conserved links between mitochondrial metabolism and aging.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12817237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145802892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dacarbazine as a Positive Control in Melanoma Cell Lines (A375, SK-MEL-103, 1205Lu) and a Human Ex Vivo Skin Model 达卡巴嗪作为黑色素瘤细胞系（A375, SK-MEL-103, 1205Lu）和人离体皮肤模型的阳性对照

IF 2.6 3区生物学

Advanced biology Pub Date : 2026-01-20 DOI: 10.1002/adbi.202500532

Marcel Nani Leite, Natália Aparecida de Paula Rios, Juliana Santos Rosa Viegas, Maria Vitória Lopes Badra Bentley, Leandra Náira Zambelli Ramalho, Enilza Maria Espreafico, Marco Andrey Cipriani Frade

{"title":"Dacarbazine as a Positive Control in Melanoma Cell Lines (A375, SK-MEL-103, 1205Lu) and a Human Ex Vivo Skin Model","authors":"Marcel Nani Leite, Natália Aparecida de Paula Rios, Juliana Santos Rosa Viegas, Maria Vitória Lopes Badra Bentley, Leandra Náira Zambelli Ramalho, Enilza Maria Espreafico, Marco Andrey Cipriani Frade","doi":"10.1002/adbi.202500532","DOIUrl":"10.1002/adbi.202500532","url":null,"abstract":"<p>One important step for evaluating and selecting a drug is toxicity studies, which are responsible for eliminating molecules that are considered promising for treating a certain disease based on their effectiveness in clinical studies, but are unsafe to go to the pharmaceutical market. We proposed an evaluation of dacarbazine as a positive control in toxicity effects in the context of macro- and micro effects represented by tissue and cell responses. A resazurin assay is used to evaluate cytotoxicity in cells (melanoma cells A375, Sk-Mel-103, and 1205Lu; immortalized skin cells HaCat and 3T3), and hematoxylin/eosin staining and TUNEL staining are used in skin explants. There is no toxicity demonstrated in the immortalized cells at the studied concentrations, whereas in the melanoma cells, A375 is the most sensitive to dacarbazine, with a high toxicity at all concentrations over 72 h (<i>p</i> < 0.05), Sk-Mel-103 showed toxicity effects only at 200 µg/mL, and 1205Lu showed no evidence of toxicity. Histological data showed that the entire skin structure of the explants is preserved, and no apoptotic cells are observed. Thus, we can conclude that cell lines behave differently when exposed to a drug, in this case Dacarbazine proved to be a good control for toxicity tests.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12817238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0