Advanced biology最新文献_第4页

Label-Free Detection of Lipid Accumulation in Cells Using Magnetic Levitation. 利用磁悬浮技术无标记检测细胞内脂质积累。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-27 DOI: 10.1002/adbi.202200142

Kazim Kerim Moncal, Laeya Abdoli Najmi, Rakhi Gupta, Malavika Ramarao, Joshua W Knowles, Chong Y Park, Naside Gozde Durmus

{"title":"Label-Free Detection of Lipid Accumulation in Cells Using Magnetic Levitation.","authors":"Kazim Kerim Moncal, Laeya Abdoli Najmi, Rakhi Gupta, Malavika Ramarao, Joshua W Knowles, Chong Y Park, Naside Gozde Durmus","doi":"10.1002/adbi.202200142","DOIUrl":"https://doi.org/10.1002/adbi.202200142","url":null,"abstract":"Dysfunction in adipose tissue can cause serious health problems, including obesity, type-2 diabetes, and cardiovascular disease, significantly reducing human life expectancy. Differences in differentiation and lipid accumulation in adipocytes reflect their functional status, making it important to characterize adipocytes by monitoring biophysical changes during adipogenic differentiation. However, there is currently no specific cell surface marker to separate mature adipocytes from non-adipose cells based on their lipid content, and separation of mature adipocytes is challenging due to handling limitations without fixation, antibody staining, or particle conjugation. Here, we report a biomarker-free, magnetic levitation-based method to detect density changes and quantify the accumulation of lipid-rich droplets within differentiating adipogenic cells. Magnetic levitation revealed density changes within preadipocytes differentiating towards mature adipocytes, with density decreasing over time as cells accumulated lipids. We then used lipid droplets as an intracellular marker to quantify lipid accumulation in single adipocytes during adipogenesis. The significant density changes correlated with cell morphology and lipid droplet morphology within the cytoplasm. For the first time, free-floating lipid vesicle density was measured using magnetic levitation. This unique method enables efficient detection and quantification of dynamically evolving lipid droplets in cells, proving beneficial for modeling lipid storage-related diseases and drug screening applications.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2200142"},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-X-C Motif Ligand 1 Induces Cell Migration by Upregulating ICAM-1 Expression by Activating PI3K/Akt and NF-κB Signaling Pathway in Liver Cancer C-X-C Motif配体1通过激活PI3K/Akt和NF-κB信号通路上调ICAM-1表达诱导肝癌细胞迁移

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-27 DOI: 10.1002/adbi.202400295

Yi-Hsin Chen, Chih-Chun Chu, Ju-Fang Liu, Hong-Shiee Lai, You-Tzung Chen

{"title":"C-X-C Motif Ligand 1 Induces Cell Migration by Upregulating ICAM-1 Expression by Activating PI3K/Akt and NF-κB Signaling Pathway in Liver Cancer","authors":"Yi-Hsin Chen, Chih-Chun Chu, Ju-Fang Liu, Hong-Shiee Lai, You-Tzung Chen","doi":"10.1002/adbi.202400295","DOIUrl":"10.1002/adbi.202400295","url":null,"abstract":"Human hepatocellular carcinoma (HCC) is the most common liver cancer and the third leading cause of cancer-related deaths worldwide. HCC is a malignant tumor that can lead to intrahepatic and extrahepatic metastases. Intercellular adhesion molecule 1 (ICAM-1) is involved in cancer metastasis. ICAM-1 enhances cell-cell interactions by promoting adhesion and facilitating cell movement within the extracellular matrix. Moreover, ICAM-1 is more abundant in cancerous hepatocytes than in non-cancerous ones. Chemokine (C-X-C motif) ligand 1 (CXCL1) is found in diverse cancers, including melanoma, breast, lung, pancreatic, colorectal, and prostate. Several studies show a correlation between CXCL1 overexpression and poor prognosis in cancer. CXCL1 has been identified as a candidate gene that could function as a clinically relevant biomarker in HCC. However, the role of CXCL1 in cancer metastasis in HCC is poorly delineated. In this study, Gene Expression Omnibus (GEO) database analysis revealed a positive correlation between CXCL1 level and the progression and metastasis of hepatocellular carcinoma patients. CXCL1 treatment facilitates cell movement through inducing ICAM-1 expression. The Phosphoinositide 3-kinase (PI3K)/Akt/Nuclear Factor kappa B (NF-kB) signaling pathway plays a crucial role in CXCL1-regulated ICAM-1 production and cell motility. Thus, CXCL1 represents a promising therapeutic target for treating metastatic hepatocellular carcinoma.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone 通过振荡流体流动的骨细胞机械负荷调节早期PC-3前列腺癌骨转移。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-19 DOI: 10.1002/adbi.202400824

Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Burton Yang, Yu Sun, Lidan You

{"title":"Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone","authors":"Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Burton Yang, Yu Sun, Lidan You","doi":"10.1002/adbi.202400824","DOIUrl":"10.1002/adbi.202400824","url":null,"abstract":"Bone metastasis is a devastating complication for advanced-stage prostate cancer patients. Osteocytes, as the primary mechanosensors in bone, have been recently investigated for their role in prostate cancer bone metastasis. In vivo findings show potential benefits of exercise as a preventative intervention strategy for bone metastasis. In contrast, in vitro studies indicate direct prostate cancer-osteocyte interactions under mechanical loading promote prostate cancer growth and migration. These findings are not consistent with in vivo results and may be more reflective of late-stage metastatic colonization. Here, the role of flow-stimulated osteocytes during early-stage bone metastasis, particularly prostate cancer-endothelial interactions, is examined. Flow-stimulated osteocytes reduce PC-3 prostate cancer cell adhesion and trans-endothelial migration by 32.3% and 40% compared to static controls. Both MLO-Y4 and primary murine osteocytes under mechanical loading regulate the extravasation distance and frequency of PC-3 cells in a microfluidic tissue model. Application of vascular cellular adhesion molecule 1 (VCAM-1) neutralizing antibody abolishes the difference in cancer cell adhesion, extravasation frequency, and number of extravasated PC-3 cells between static and flow-stimulated groups. Taken together, the role of osteocytes in early-stage bone metastasis using PC-3 cells as a model is demonstrated here, bridging the gap between in vitro and in vivo findings.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Naringin and Zinc Treatment on Biochemical, Molecular, and Histological Alterations in Stomach and Pancreatic Tissues of STZ-Induced Diabetic Rats 柚皮苷和锌对stz诱导的糖尿病大鼠胃和胰腺组织生化、分子和组织学改变的影响。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400688

Al-Shimaa M. Abas, Mohamed H. Sherif, Sarah Ibrahim

{"title":"Effects of Naringin and Zinc Treatment on Biochemical, Molecular, and Histological Alterations in Stomach and Pancreatic Tissues of STZ-Induced Diabetic Rats","authors":"Al-Shimaa M. Abas, Mohamed H. Sherif, Sarah Ibrahim","doi":"10.1002/adbi.202400688","DOIUrl":"10.1002/adbi.202400688","url":null,"abstract":"Diabetes mellitus is a chronic metabolic disorder that affects multiple organs, including the stomach. This research examines the effects of naringin and/or zinc on stomach and pancreatic tissues of streptozotocin-induced diabetic rats. Type 2 diabetes is induced by intraperitoneal injection of nicotinamide and streptozotocin. Three weeks after diabetes induction, rats receive eight weeks of treatment. Malondialdehyde and total antioxidant capacity are estimated colorimetrically. Asprosin and P-selectin levels are assessed via ELISA. Quantitative RT-PCR analysis of nuclear factor kappa B (NF-кB), peroxisome proliferator-activated receptor gamma (PPAR γ), and nuclear factor erythroid 2-related factor 2 (Nrf-2) genes is carried out. Tumor necrosis factor-alpha (TNF-α) is assessed immunohistochemically, and stomach and pancreatic tissues are examined histologically. Combined naringin and zinc treatment significantly reduces gastric Malondialdehyde, serum asprosin, and P-selectin levels in serum, stomach, and pancreas compared to diabetic rats. Additionally, gastric NF-кB expression is significantly lower, while PPAR γ and Nrf-2 expressions are significantly higher compared to diabetic rats. Immunohistochemical analysis and histopathological examination confirm these findings. In conclusion, combined naringin and zinc treatment significantly improves gastric alterations in diabetic rats by reducing oxidative stress and inflammation. Nonetheless, it shows no additional impacts on pancreatic tissue compared to naringin or zinc alone.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics. 推进眼科研究和治疗的当前和未来角膜芯片模型。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400571

Minju Kim, Kanghoon Choi, Amy Lin, Jungkyu Kim

{"title":"Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.","authors":"Minju Kim, Kanghoon Choi, Amy Lin, Jungkyu Kim","doi":"10.1002/adbi.202400571","DOIUrl":"https://doi.org/10.1002/adbi.202400571","url":null,"abstract":"Corneal blindness remains a significant global health challenge, with limited treatment options due to donor tissue scarcity outside of the United States and inadequate in vitro models. This review analyzes the current state of cornea chip technology, addressing fundamental challenges and exploring future directions. Recent advancements in biomaterials and fabrication techniques are discussed that aim to recapitulate the complex structure and function of the human cornea, including the multilayered epithelium, organized stroma, and functional endothelium. The review highlights the potential of the cornea chips to revolutionize ocular research by offering more predictive and physiologically relevant models for drug screening, disease modeling, and personalized medicine. Current designs, their applications in studying drug permeability, barrier function, and wound healing, and their limitations in replicating native corneal architecture, are examined. Key challenges include integrating corneal curvature, basement membrane formation, and innervation. Applications are explored in modeling diseases like keratitis, dry eye disease, keratoconus, and Fuchs' endothelial dystrophy. Future directions include incorporating corneal curvature using hydraulically controlled systems, using patient-derived cells, and developing comprehensive disease models to accelerate therapy development and reduce reliance on animal testing.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400571"},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome Size Is Positively Correlated with Lifespan in Mammals but Negatively Correlated with Lifespan in Birds 哺乳动物的蛋白质组大小与寿命呈正相关，而鸟类的蛋白质组大小与寿命负相关。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400633

Juliano Morimoto, Zuzanna Pietras

引用次数: 0

Cancer Cell and Cancer-Associated Fibroblast Communication-Mediated Molecular Subtypes Portray Non-Inflamed Tumor Microenvironment and Guide the Precision Treatment of Bladder Cancer 癌细胞和癌症相关成纤维细胞通讯介导的分子亚型描绘非炎症肿瘤微环境并指导膀胱癌的精确治疗。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400434

Shenglin Gao, Chuan Liu, Lixin Mao, Yin Chen, Xiaokai Shi, Chuang Yue, Shouchun Li, Xihu Qin

{"title":"Cancer Cell and Cancer-Associated Fibroblast Communication-Mediated Molecular Subtypes Portray Non-Inflamed Tumor Microenvironment and Guide the Precision Treatment of Bladder Cancer","authors":"Shenglin Gao, Chuan Liu, Lixin Mao, Yin Chen, Xiaokai Shi, Chuang Yue, Shouchun Li, Xihu Qin","doi":"10.1002/adbi.202400434","DOIUrl":"10.1002/adbi.202400434","url":null,"abstract":"Cancer-associated fibroblasts (CAFs) drive tumor progression through restructuring of the tumor microenvironment. This investigation aim to elucidate the function of molecular subtypes (MS) derived from cancer cells communication with CAFs, depicting the hallmarks of the tumor microenvironment and precise bladder cancer (BLCA) treatment. The BLCA data from TCGA and several external sources are utilized to generate a novel ligand, receptor, and transcription factor (LRT) associated molecular subtype and their corresponding score (LRT score). The LRT-mediated molecular subtype is identified via unsupervised clustering. LRT score is measured by principal component analysis. Then, the association of LRT clusters to established MS, immunophenotypes, and medical endpoints, together with BLCA treatment strategies is investigated. Two LRT clusters (A and B) are identified. LRT cluster (LRT score) can precisely propose immunophenotypes, classical MS, clinical outcomes, and BLCA therapeutic strategies. Cluster B (Low LRT score) represent a basal subtype and inflamed phenotype specified by high immunity against tumors and unfavorable clinical outcomes. Furthermore, it is highly sensitive to cancer immunotherapy; however, it has low sensitivity to antiangiogenic and targeted therapies. The novel LRT clusters with a strong association with biological characteristics and precise BLCA treatment strategies are derived from the communication between cancer cells and cancer-associated fibroblasts. The LRT may be a useful clinician tool for developing individualized treatment strategies.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Luteolin in Inhibiting Prostaglandin-Endoperoxide Synthase 2 to Relieve Neointimal Hyperplasia in Arteriovenous Fistula 木犀草素抑制前列腺素内过氧化物合酶2缓解动静脉瘘新生内膜增生的作用。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400437

Ruibin Zhang, Wei Li, Jihua Yang, Xiujie Fan, Huili Fan, Wei Li

{"title":"The Role of Luteolin in Inhibiting Prostaglandin-Endoperoxide Synthase 2 to Relieve Neointimal Hyperplasia in Arteriovenous Fistula","authors":"Ruibin Zhang, Wei Li, Jihua Yang, Xiujie Fan, Huili Fan, Wei Li","doi":"10.1002/adbi.202400437","DOIUrl":"10.1002/adbi.202400437","url":null,"abstract":"This study aims to investigate the role and mechanism of luteolin in inflammation and phenotypic switch of vascular smooth muscle cells (VSMCs) in an arteriovenous fistula (AVF) model, for providing a potential agent for the prevention and therapy of AVF neointimal hyperplasia. In vivo, an AVF model is created in Sprague Dawley rats. In vitro, rat VSMCs are treated with platelet-derived growth factor-BB (PDGF-BB) to induce the phenotypic switch of VSMCs. Histological AVF changes are analyzed using hematoxylin-eosin. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) are utilized to detect prostaglandin-endoperoxide synthase 2 (PTGS2) expression. In vivo, luteolin inhibits neointima formation and reduces vimentin, α-SMA, MCP-1, MMP-9, TNF-α, and IL-6 levels. In vitro, under PDGF-BB treatment, luteolin inhibits proliferation and migration and reduces TNF-α, vimentin, α-SMA, MCP-1, MMP-9, and IL-6 levels in VSMCs. In rat AVF tissues, PTGS2 expression is increased. Luteolin inhibits PTGS2 expression in vivo and in vitro. PTGS2 overexpression reverses the role of luteolin in extracellular matrix protein expression, proliferation, inflammation, and migration in VSMCs treated with PDGF-BB. Altogether, in the AVF, luteolin inhibits proliferation, migration, the phenotypic switch of VSMCs, neointima formation, and the inflammatory response through inhibiting PTGS2 expression.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels 透明质酸微凝胶中无细胞蛋白合成过程中调节蛋白固定化。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400668

Anika Kaufmann, Kateryna Ivanova, Julian Thiele

{"title":"Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels","authors":"Anika Kaufmann, Kateryna Ivanova, Julian Thiele","doi":"10.1002/adbi.202400668","DOIUrl":"10.1002/adbi.202400668","url":null,"abstract":"Cell-like platforms are being studied intensively for their application in synthetic biology to mimic aspects of life in an artificial environment. Here, micrometer-sized, bifunctional microgels are used as an experimental platform to investigate the interplay of cell-free protein synthesis (CFPS) and in situ protein accumulation inside the microgel volume. In detail, microgels made of hyaluronic acid (HA) are first modified with different amounts of nitrilotriacetic acid (NTA) moieties to characterize the capability and maximum capacity of binding His-tag modified GFP. CFPS is optimized for the system used here, particularly when using a linear DNA template. Afterward, HA-microgels are functionalized with the linear DNA template and Ni2+-activated NTA moieties to bind in situ synthesized GFP-His. CFPS and parallel protein accumulation within the microgels are observed over time to determine the GFP-His binding to the microgel platform. With this approach, the study presents the first steps for a platform to study the temporal-spatial regulation of protein synthesis by tailored protein binding or release from the microgel matrix-based reaction environment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIF11 Inhibition Induces Retinopathy Progression by Affecting Photoreceptor Cell Ciliogenesis and Cell Cycle Regulation in Development KIF11抑制通过影响感光细胞纤毛发生和细胞周期调节诱导视网膜病变进展。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400748

Yue Xu, Jie Chen, Xin-Yao Wang, Min-Hui Huang, Xiang Wei, Xin-Rui Luo, Ya-Lan Wei, Zhen-Yu She

{"title":"KIF11 Inhibition Induces Retinopathy Progression by Affecting Photoreceptor Cell Ciliogenesis and Cell Cycle Regulation in Development","authors":"Yue Xu, Jie Chen, Xin-Yao Wang, Min-Hui Huang, Xiang Wei, Xin-Rui Luo, Ya-Lan Wei, Zhen-Yu She","doi":"10.1002/adbi.202400748","DOIUrl":"10.1002/adbi.202400748","url":null,"abstract":"Microcephaly with or without chorioretinopathy, lymphedema, or impaired intellectual development (MCLMR; OMIM 152950) is a rare autosomal dominant disorder, which is primarily characterized by defects in the central nervous system and retinal developmental anomalies. Kinesin-5 KIF11 has been discovered as a major causative gene for MCLMR. It has been well established that KIF11 is essential for microtubule organization, centrosome separation, and spindle assembly during mitosis. However, cellular and molecular mechanisms in the physiopathology of MCLMR remain largely unknown. In this study, KIF11-inhibition mouse models are generated, which reveal that chemical inhibition of KIF11 results in defects in retinal development, the formation of rosettes, photoreceptor ciliary alterations, and vision loss. Furthermore, it is demonstrated that KIF11 is essential for the formation, organization, and maintenance of primary cilia in photoreceptor cells, which further contributes to the organization of photoreceptor cells and the development of the retina. Using the developing mouse embryos as a model, it is revealed that KIF11 inhibition induces the formation of monopolar spindle and mitotic arrest, which further results in tetraploidy and apoptotic cell death. These findings uncover cellular mechanisms underlying the loss-of-function of KIF11 and retinopathy in MCLMR and further support the functions of KIF11 in development.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0