Advanced biology最新文献_第4页

Cancer Cell and Cancer-Associated Fibroblast Communication-Mediated Molecular Subtypes Portray Non-Inflamed Tumor Microenvironment and Guide the Precision Treatment of Bladder Cancer

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400434

Shenglin Gao, Chuan Liu, Lixin Mao, Yin Chen, Xiaokai Shi, Chuang Yue, Shouchun Li, Xihu Qin

{"title":"Cancer Cell and Cancer-Associated Fibroblast Communication-Mediated Molecular Subtypes Portray Non-Inflamed Tumor Microenvironment and Guide the Precision Treatment of Bladder Cancer","authors":"Shenglin Gao, Chuan Liu, Lixin Mao, Yin Chen, Xiaokai Shi, Chuang Yue, Shouchun Li, Xihu Qin","doi":"10.1002/adbi.202400434","DOIUrl":"10.1002/adbi.202400434","url":null,"abstract":"Cancer-associated fibroblasts (CAFs) drive tumor progression through restructuring of the tumor microenvironment. This investigation aim to elucidate the function of molecular subtypes (MS) derived from cancer cells communication with CAFs, depicting the hallmarks of the tumor microenvironment and precise bladder cancer (BLCA) treatment. The BLCA data from TCGA and several external sources are utilized to generate a novel ligand, receptor, and transcription factor (LRT) associated molecular subtype and their corresponding score (LRT score). The LRT-mediated molecular subtype is identified via unsupervised clustering. LRT score is measured by principal component analysis. Then, the association of LRT clusters to established MS, immunophenotypes, and medical endpoints, together with BLCA treatment strategies is investigated. Two LRT clusters (A and B) are identified. LRT cluster (LRT score) can precisely propose immunophenotypes, classical MS, clinical outcomes, and BLCA therapeutic strategies. Cluster B (Low LRT score) represent a basal subtype and inflamed phenotype specified by high immunity against tumors and unfavorable clinical outcomes. Furthermore, it is highly sensitive to cancer immunotherapy; however, it has low sensitivity to antiangiogenic and targeted therapies. The novel LRT clusters with a strong association with biological characteristics and precise BLCA treatment strategies are derived from the communication between cancer cells and cancer-associated fibroblasts. The LRT may be a useful clinician tool for developing individualized treatment strategies.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400668

Anika Kaufmann, Kateryna Ivanova, Julian Thiele

{"title":"Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.","authors":"Anika Kaufmann, Kateryna Ivanova, Julian Thiele","doi":"10.1002/adbi.202400668","DOIUrl":"https://doi.org/10.1002/adbi.202400668","url":null,"abstract":"Cell-like platforms are being studied intensively for their application in synthetic biology to mimic aspects of life in an artificial environment. Here, micrometer-sized, bifunctional microgels are used as an experimental platform to investigate the interplay of cell-free protein synthesis (CFPS) and in situ protein accumulation inside the microgel volume. In detail, microgels made of hyaluronic acid (HA) are first modified with different amounts of nitrilotriacetic acid (NTA) moieties to characterize the capability and maximum capacity of binding His-tag modified GFP. CFPS is optimized for the system used here, particularly when using a linear DNA template. Afterward, HA-microgels are functionalized with the linear DNA template and Ni2+-activated NTA moieties to bind in situ synthesized GFP-His. CFPS and parallel protein accumulation within the microgels are observed over time to determine the GFP-His binding to the microgel platform. With this approach, the study presents the first steps for a platform to study the temporal-spatial regulation of protein synthesis by tailored protein binding or release from the microgel matrix-based reaction environment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400668"},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Luteolin in Inhibiting Prostaglandin-Endoperoxide Synthase 2 to Relieve Neointimal Hyperplasia in Arteriovenous Fistula

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400437

Ruibin Zhang, Wei Li, Jihua Yang, Xiujie Fan, Huili Fan, Wei Li

{"title":"The Role of Luteolin in Inhibiting Prostaglandin-Endoperoxide Synthase 2 to Relieve Neointimal Hyperplasia in Arteriovenous Fistula","authors":"Ruibin Zhang, Wei Li, Jihua Yang, Xiujie Fan, Huili Fan, Wei Li","doi":"10.1002/adbi.202400437","DOIUrl":"10.1002/adbi.202400437","url":null,"abstract":"This study aims to investigate the role and mechanism of luteolin in inflammation and phenotypic switch of vascular smooth muscle cells (VSMCs) in an arteriovenous fistula (AVF) model, for providing a potential agent for the prevention and therapy of AVF neointimal hyperplasia. In vivo, an AVF model is created in Sprague Dawley rats. In vitro, rat VSMCs are treated with platelet-derived growth factor-BB (PDGF-BB) to induce the phenotypic switch of VSMCs. Histological AVF changes are analyzed using hematoxylin-eosin. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) are utilized to detect prostaglandin-endoperoxide synthase 2 (PTGS2) expression. In vivo, luteolin inhibits neointima formation and reduces vimentin, α-SMA, MCP-1, MMP-9, TNF-α, and IL-6 levels. In vitro, under PDGF-BB treatment, luteolin inhibits proliferation and migration and reduces TNF-α, vimentin, α-SMA, MCP-1, MMP-9, and IL-6 levels in VSMCs. In rat AVF tissues, PTGS2 expression is increased. Luteolin inhibits PTGS2 expression in vivo and in vitro. PTGS2 overexpression reverses the role of luteolin in extracellular matrix protein expression, proliferation, inflammation, and migration in VSMCs treated with PDGF-BB. Altogether, in the AVF, luteolin inhibits proliferation, migration, the phenotypic switch of VSMCs, neointima formation, and the inflammatory response through inhibiting PTGS2 expression.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIF11 Inhibition Induces Retinopathy Progression by Affecting Photoreceptor Cell Ciliogenesis and Cell Cycle Regulation in Development

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400748

Yue Xu, Jie Chen, Xin-Yao Wang, Min-Hui Huang, Xiang Wei, Xin-Rui Luo, Ya-Lan Wei, Zhen-Yu She

{"title":"KIF11 Inhibition Induces Retinopathy Progression by Affecting Photoreceptor Cell Ciliogenesis and Cell Cycle Regulation in Development","authors":"Yue Xu, Jie Chen, Xin-Yao Wang, Min-Hui Huang, Xiang Wei, Xin-Rui Luo, Ya-Lan Wei, Zhen-Yu She","doi":"10.1002/adbi.202400748","DOIUrl":"10.1002/adbi.202400748","url":null,"abstract":"Microcephaly with or without chorioretinopathy, lymphedema, or impaired intellectual development (MCLMR; OMIM 152950) is a rare autosomal dominant disorder, which is primarily characterized by defects in the central nervous system and retinal developmental anomalies. Kinesin-5 KIF11 has been discovered as a major causative gene for MCLMR. It has been well established that KIF11 is essential for microtubule organization, centrosome separation, and spindle assembly during mitosis. However, cellular and molecular mechanisms in the physiopathology of MCLMR remain largely unknown. In this study, KIF11-inhibition mouse models are generated, which reveal that chemical inhibition of KIF11 results in defects in retinal development, the formation of rosettes, photoreceptor ciliary alterations, and vision loss. Furthermore, it is demonstrated that KIF11 is essential for the formation, organization, and maintenance of primary cilia in photoreceptor cells, which further contributes to the organization of photoreceptor cells and the development of the retina. Using the developing mouse embryos as a model, it is revealed that KIF11 inhibition induces the formation of monopolar spindle and mitotic arrest, which further results in tetraploidy and apoptotic cell death. These findings uncover cellular mechanisms underlying the loss-of-function of KIF11 and retinopathy in MCLMR and further support the functions of KIF11 in development.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Decellularization Method on the Cytocompatibility and Wound Healing Capability of Human Amniotic Membrane

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400509

Haadia Tauseef, Kainat Ahmed, Faiza Chaudhary, Asmat Salim, Omair Anwar Mohiuddin

{"title":"The Impact of Decellularization Method on the Cytocompatibility and Wound Healing Capability of Human Amniotic Membrane","authors":"Haadia Tauseef, Kainat Ahmed, Faiza Chaudhary, Asmat Salim, Omair Anwar Mohiuddin","doi":"10.1002/adbi.202400509","DOIUrl":"10.1002/adbi.202400509","url":null,"abstract":"The decellularized human amniotic membrane (dHAM) has been evaluated as a biomaterial for various tissue engineering applications, notably as a skin dressing for wound healing. The decellularization process alters the composition and structure of the extracellular matrix consequently influencing its characteristics. The aim of the present study was to comparatively evaluate dHAM-E and dHAM-S prepared by enzymatic and salt solution treatment respectively for their microstructure using scanning electron microscopy (SEM), in vitro biocompatibility with mesenchymal stem cells (MSCs), and regenerative capability in full-thickness wound model in mice. The SEM results revealed increased porosity in dHAM-S and better MSC adhesion and proliferation as compared to dHAM-E. Moreover, wound healing capability assessed at day 7 and day 14 by histological analysis of the regenerated tissues indicated that the dHAM treated groups achieved greater re-epithelialization and remodeling than the untreated group. However, dHAM-S treated samples presented a more remodeled regenerated skin than the other groups. Furthermore, gene expression analysis of the regenerated skin displayed a higher expression of anti-inflammatory, proliferation, and keratinization marker genes in the dHAM treated groups. Overall, it was found that dHAMs are compatible with MSCs and improve wound healing. However, clear differences were observed in the bioactivity of the two dHAMs.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging (Adv. Biology 2/2025)

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-16 DOI: 10.1002/adbi.202570093

Ayah A. Farhat, Yazan A. Almahdi, Fatima Z. Alshuhani, Besa Xhabija

引用次数: 0

Vectors in CRISPR Gene Editing for Neurological Disorders: Challenges and Opportunities

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-14 DOI: 10.1002/adbi.202400374

Kexin Xiong, Xiaxia Wang, Caicai Feng, Kaixiang Zhang, Di Chen, Sen Yang

{"title":"Vectors in CRISPR Gene Editing for Neurological Disorders: Challenges and Opportunities","authors":"Kexin Xiong, Xiaxia Wang, Caicai Feng, Kaixiang Zhang, Di Chen, Sen Yang","doi":"10.1002/adbi.202400374","DOIUrl":"10.1002/adbi.202400374","url":null,"abstract":"Diseases of the nervous system are recognized as the second leading cause of death worldwide. The global prevalence of neurological diseases, such as Huntington's disease, Alzheimer's disease, and Parkinson's disease has seen a significant rise due to the increasing proportion of the aging population. The discovery of the clustered regularly interspaced short palindromic repeats (CRISPR) genome editing technique has paved way for universal neurological diseases treatment. However, finding a safe and effective method to deliver CRISPR gene-editing tools remains a main challenge for genome editing therapies in vivo. Adeno-associated virus (AAV) is currently one of the most commonly used vector systems, but some issues remain unresolved, including capsid immunogenicity, off-target mutations, and potential genotoxicity. To address these concerns, researchers are actively encouraging the development of new delivery systems, like virus-like particles and nanoparticles. These novel systems have the potential to enhance targeting efficiency, thereby offering possible solutions to the current challenges. This article reviews CRISPR delivery vectors for neurological disorders treatment and explores potential solutions to overcome limitations in vector systems. Additionally, the delivery strategies of CRISPR systems are highlighted as valuable tools for studying neurological diseases, and the challenges and opportunities that these vectors present.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broadening the Healthy Aging Paradigm: Inclusion of Gestation, Development, and Reproductive Health

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-14 DOI: 10.1002/adbi.202400481

Lan Yao, John Eikelboom, Andrew Macnab, Zhuo Yu, Yanhong Cao, Wei Wei, Shuqiu Sun, Asma Khalil, William C. Cho, Farah Naz Qamar, Peter Kremsner, Miquel Oliu-Barton, Lishi Wang, Ivan Sisa, Florian Marks, Cong-Yi Wang, J. Carolyn Graff, Scott C. Howard, Tianshu Gu, Marcello Maida, Edward V Prochownik, Tapfumanei Mashe, Alyce D Fly, Yang Hui, Andrea B. Maier, Sami Aleya, Dianjun Sun, Gang Wang, Evelyne Yehudit Bischof, Lotfi Aleya, Weikuan Gu

{"title":"Broadening the Healthy Aging Paradigm: Inclusion of Gestation, Development, and Reproductive Health","authors":"Lan Yao, John Eikelboom, Andrew Macnab, Zhuo Yu, Yanhong Cao, Wei Wei, Shuqiu Sun, Asma Khalil, William C. Cho, Farah Naz Qamar, Peter Kremsner, Miquel Oliu-Barton, Lishi Wang, Ivan Sisa, Florian Marks, Cong-Yi Wang, J. Carolyn Graff, Scott C. Howard, Tianshu Gu, Marcello Maida, Edward V Prochownik, Tapfumanei Mashe, Alyce D Fly, Yang Hui, Andrea B. Maier, Sami Aleya, Dianjun Sun, Gang Wang, Evelyne Yehudit Bischof, Lotfi Aleya, Weikuan Gu","doi":"10.1002/adbi.202400481","DOIUrl":"10.1002/adbi.202400481","url":null,"abstract":"The prevailing focus of lifespan health research has predominantly centered on “healthy aging”. This oversight may hinder the understanding of health across the lifespan, as disorders in earlier stages can substantially impact overall health and longevity. Aging, conceptually, begins at gestation. The trajectory of an individual's health is influenced from the earliest stages of life, where adverse conditions can set a foundation for lifelong health challenges. For example, suboptimal conditions during gestation leading to premature birth can predispose individuals to various health issues later in life. Additionally, precocious puberty defined as the onset of sexual maturity before eight years of age or early menopause—occurring before 50 years of age requires medical intervention and is indicative of atypical aging processes. To address these critical gaps in lifespan health research, the expansion of medical lexicons and research categorizations is advocated to include “healthy gestation,” “healthy development,” and “healthy reproduction” alongside “healthy aging.” This broader terminology will enable a more comprehensive investigation of disorders at all life stages. An integrative approach underscores the interconnectedness of all life stages and the continuous nature of aging, advocating for a seamless continuum in health research and interventions from gestation through late adulthood.\u0000","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Extrachromosomal DNA in Cancer: Biogenesis, Methodologies, Functions, and Therapeutic Potential

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-13 DOI: 10.1002/adbi.202400433

Xudong Mao, Guocheng Rao, Gonghui Li, Shihan Chen

{"title":"Insights into Extrachromosomal DNA in Cancer: Biogenesis, Methodologies, Functions, and Therapeutic Potential","authors":"Xudong Mao, Guocheng Rao, Gonghui Li, Shihan Chen","doi":"10.1002/adbi.202400433","DOIUrl":"10.1002/adbi.202400433","url":null,"abstract":"Originating from, but independent of, linear chromosomes, extrachromosomal DNA (ecDNA) exists in a more active state of transcription and autonomous replication. It plays a crucial role in the development of malignancies and therapy resistance. Since its discovery in eukaryotic cells more than half a century ago, the biological characteristics and functions of ecDNA have remained unclear due to limitations in detection methods. However, recent advancements in research tools have transformed ecDNA research. It is believed that ecDNA exhibits greater activity in the abnormal amplification of oncogenes, thereby driving cancer progression through their overexpression. Notably, compared to linear DNA, ecDNA can also function as a genomic element with regulatory roles, including both trans- and cis-acting functions. Its critical roles in tumorigenesis, evolution, progression, and drug resistance in malignant tumors are increasingly recognized. This review provides a comprehensive summary of the evolutionary context of ecDNA and highlights significant progress in understanding its biological functions and potential applications as a therapeutic target in malignant tumors.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Considering the Risks and Costs of Solid Organ Xenotransplantation

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-13 DOI: 10.1002/adbi.202400453

Catharine E. Krebs, Janine McCarthy, Kristie Sullivan, James Craner, Brendan Parent, Ann Lam

{"title":"Considering the Risks and Costs of Solid Organ Xenotransplantation","authors":"Catharine E. Krebs, Janine McCarthy, Kristie Sullivan, James Craner, Brendan Parent, Ann Lam","doi":"10.1002/adbi.202400453","DOIUrl":"10.1002/adbi.202400453","url":null,"abstract":"The standard treatment for end-stage organ failure is transplantation, but demand for organs has always vastly outstripped supply. Discussions are ongoing about the feasibility of addressing the organ shortage through measures like increasing organ donations, improving post-transplant outcomes, and xenotransplantation. This paper examines the rationale, risks, and costs of xenotransplantation, such as xenozoonoses, creating a new form of industrialized animal farming, abandoning animal ethics principles, and the opportunity costs of investing finite research dollars in xenotransplantation instead of investing in more viable strategies. Alternative strategies that can ethically and effectively address the demand for heart, kidney, and other transplants are recommended: Improving disease prevention and management to reduce demand for transplant organs, improving transplantation methods, and systemic changes to donor policies and organ recovery methods to increase overall supply. Upon careful exploration of the full landscape of organ transplantation, it is considered whether these alternative strategies that do not impose the definite harms and significant risks of xenotransplantation are the most ethical and effective means to increase life-saving options and improve clinical outcomes for patients in organ failure.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0