Luteolin Regulates Mitophagy to Alleviate Myocardial Ischemia-Reperfusion Injury via Sirt3/Foxo3a Pathway.

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Li Yan, Lei Liang, Qiling Gou, Haoyu Wu, Mengya Dong, Hao Chen, Jiayu Diao
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Abstract

Luteolin (LUT) belongs to a kind of flavonoid, which has protective effects on myocardial ischemia/reperfusion (I/R) injury. Sirt3 is located in mitochondria and interacts with Foxo3a to protect mitochondrial function against stress. Mitophagy is an important form of mitochondrial quality control. However, whether LUT regulates mitophagy to alleviate myocardial I/R injury via the Sirt3/Foxo3a pathway is rarely reported. In this study, 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP) is used to inhibit the Sirt3/Foxo3a pathway. Male adult rats are divided into four groups: Sham group, I/R group, I/R+LUT group, and I/R+LUT+3-TYP group. The I/R rats model is established by ligating the left anterior descending coronary artery for 30 min, then releasing the ligature for 24 h. Indexes of left ventricular function, myocardial damage, oxidative stress, and mitophagy are detected. It is found that LUT treatment activated Sirt3/Foxo3a pathway, improves left ventricular function, decreases myocardial infarction size, inhibits myocardial apoptosis and oxidative stress, and initiates mitophagy in I/R rats. Moreover, these protective effects of LUT are weakened when Sirt3 is inhibited. Together, LUT regulates mitophagy to alleviate myocardial I/R injury via the Sirt3/Foxo3a pathway.

木犀草素通过Sirt3/Foxo3a通路调节线粒体自噬减轻心肌缺血再灌注损伤
木犀草素(lutein, LUT)属于类黄酮的一种,对心肌缺血/再灌注(I/R)损伤具有保护作用。Sirt3位于线粒体中,并与Foxo3a相互作用以保护线粒体功能免受应激。线粒体自噬是线粒体质量控制的一种重要形式。然而,LUT是否通过Sirt3/Foxo3a途径调控线粒体自噬从而减轻心肌I/R损伤的报道很少。本研究使用3-(1h -1,2,3-三唑-4-基)吡啶(3- typ)抑制Sirt3/Foxo3a通路。雄性成年大鼠分为Sham组、I/R组、I/R+LUT组、I/R+LUT+3-TYP组。结扎左冠状动脉前降支30min后解除结扎24h,建立I/R大鼠模型,检测左心室功能、心肌损伤、氧化应激、线粒体自噬等指标。结果发现,LUT处理激活Sirt3/Foxo3a通路,改善I/R大鼠左心室功能,减小心肌梗死面积,抑制心肌凋亡和氧化应激,启动线粒体自噬。此外,当Sirt3被抑制时,LUT的这些保护作用被削弱。总之,LUT通过Sirt3/Foxo3a途径调节线粒体自噬以减轻心肌I/R损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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