Advanced biology最新文献

{"title":"Modelling Atherosclerotic Plaque Cap Mechanics: Microcalcifications Reduce Mechanical Properties in Mesenchymal Stromal Cell-Based Model","authors":"Imke L. Jansen,&nbsp;Deniz Şahin,&nbsp;Frank J.H. Gijsen,&nbsp;Eric Farrell,&nbsp;Kim van der Heiden","doi":"10.1002/adbi.202500106","DOIUrl":"10.1002/adbi.202500106","url":null,"abstract":"<p>Rupture of atherosclerotic plaque caps is the cause of many disabling or lethal cardiovascular events, such as stroke and myocardial infarction. Microcalcifications (&lt;50 µm) have been shown, in computational models, to affect the biomechanical stability of the cap. The current study aims to develop a tissue-engineered model of the atherosclerotic fibrous cap with microcalcifications produced by mesenchymal stromal cells (MSCs). Human MSCs are seeded in fibrin gels and cultured for 2 weeks in medium supplemented with TGF-β1 to induce smooth muscle cell differentiation and collagenous matrix formation. Afterward, mineralizing medium stimulates microcalcification formation for an additional 4 weeks. Tissue-engineered structures are imaged after culture with second harmonic generation microscopy with a hydroxyapatite probe, showing collagenous matrix with microcalcifications. Mechanical characterization shows the effect of microcalcifications on global tissue mechanics, as the ultimate stress at rupture of the tissue is significantly lower compared to control tissues. The amount of calcification, determined by histological analysis, is correlated to the decrease in ultimate tensile stress, with a higher amount of microcalcification resulting in weakened mechanical properties. The developed tissue-engineered plaque cap model with biologically formed collagenous matrix and microcalcifications offers valuable insight into the impact of microcalcifications on biomechanical stability.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The c-di-GMP Metabolic Gene pdeN Interacts with LacY and ManZ to Modulate Biofilm Formation in Avian Pathogenic Escherichia coli.","authors":"Zhihao Wang, Xiaolong Lv, Lanfang Kong, Saqib Nawaz, Chuanyan Che, Zhaoguo Chen, Huifang Yin, Cuiqin Huang, Yinli Bao, Wei Jiang, Xiangan Han","doi":"10.1002/adbi.202500190","DOIUrl":"https://doi.org/10.1002/adbi.202500190","url":null,"abstract":"<p><p>Bis-(3'-5')-cyclic diguanylic acid (c-di-GMP), a ubiquitous secondary messenger, affects multiple biological characteristics, including biofilm formation in avian pathogenic Escherichia coli (APEC). C-di-GMP is synthesized by diguanylate cyclase harboring a GGDEF domain and degraded by phosphodiesterase harboring either an EAL or an HD-GYP domain. However, the roles of PdeN, encoding a CSS-EAL domain, are uncharacterized. In this study, it is demonstrated that lacking pdeN significantly promotes biofilm formation and reduces the motility of the clinically isolated APEC O2 serotype strain DE17. In addition, macrocolony morphotypes showed that the ΔpdeN strain exhibits increasing production of curli fibers and cellulose, which is consistent with the results of RNA-seq and qPCR. Further exploration shows that lactose permease LacY and mannose permease subunit ManZ interact with PdeN. Infection experiments show that lacking pdeN significantly reduced the release of LDH in HD-11 cells and adhesion capacity to DF-1 cells. In conclusion, c-di-GMP metabolic gene pdeN involves biofilm formation and pathogenicity of APEC. Besides, it interacts with LacY and ManZ. Those results provide a basis for the prevention and control of APEC from the perspective of biofilm and carbohydrate metabolism.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00190"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications","authors":"Manon Miran,&nbsp;Kieu Ngo,&nbsp;David Buob,&nbsp;Hanna Debiec,&nbsp;Pierre Ronco,&nbsp;Guillaume Perry","doi":"10.1002/adbi.202500108","DOIUrl":"10.1002/adbi.202500108","url":null,"abstract":"<p>Chronic kidney disease (CKD) affects over 850 million individuals worldwide, often progressing to stages requiring dialysis or kidney transplants. Central to kidney function is the glomerular filtration barrier (GFB), which selectively filters waste while retaining essential proteins. Traditional models, including animal studies and 2D cell cultures, fail to fully replicate the GFB's complexity, limiting CKD research. Recent developments in microphysiological systems (MPS), particularly microphysiological glomerular filtration barriers (MPGFB), provide more accurate in vitro models for studying kidney diseases and evaluating therapies. MPGFB systems use organ-on-chip technology to integrate podocytes and glomerular endothelial cells within confined microfluidic environments, closely mimicking GFB's dynamic in vivo conditions. This setup enables detailed permeability analysis, aiding in research on disease mechanisms and drug toxicity. Furthermore, using human-induced pluripotent stem cells in MPGFB platforms allows patient-specific studies, enhancing insights into genetic kidney disorders. This review first examines the GFB's structure and function, focusing on its cellular and extracellular matrix components. It then discusses biological and engineering approaches to MPGFB fabrication, covering materials, 3D design, and flow control. The review concludes with MPGFB applications in disease modeling and drug testing, and addresses improvements needed for refining MPGFB as a key tool in kidney disease research and treatment.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chaperone-Mediated Autophagy: A Critical Regulator of Neuroinflammation and Neurodegeneration","authors":"Ying-ying Han,&nbsp;Xin-yue Huang,&nbsp;Ying Su,&nbsp;Jing-jing Ma,&nbsp;Jin Wu","doi":"10.1002/adbi.202500191","DOIUrl":"10.1002/adbi.202500191","url":null,"abstract":"<p>Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by hallmark pathological features such as the accumulation of misfolded proteins and neuroinflammation. Chaperone-mediated autophagy (CMA), a selective lysosomal pathway, facilitates the degradation of proteins containing KFERQ-like motifs via the receptor lysosome-associated membrane protein type 2A (LAMP2A). In the recent review, the pivotal role of CMA in regulating proteostasis and modulating inflammatory responses is highlighted. This commentary explores the multifaceted roles of CMA in neurodegenerative disease progression, emphasizing its involvement in age-related decline, feedback loops between CMA dysregulation and neurodegeneration, and potential as a therapeutic target. Emerging CMA activators and the challenges of modulating CMA for clinical use are also discussed.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Cellular Effects of GALC Dosing in Enzyme Replacement Therapy for Krabbe Disease Supports the Role of Nanomedicine","authors":"Ambra Del Grosso,&nbsp;Sara Carpi,&nbsp;Laura Colagiorgio,&nbsp;Miriam De Sarlo,&nbsp;Mariacristina Gagliardi,&nbsp;Marco Cecchini","doi":"10.1002/adbi.202500147","DOIUrl":"10.1002/adbi.202500147","url":null,"abstract":"<p>Krabbe disease (KD) is a lysosomal storage disorder characterized by severe neurodegeneration and demyelination. It is caused by mutations in the galactosylceramidase (GALC) gene, leading to the accumulation of psychosine, a neurotoxic metabolite. This study presents an optimized workflow for the production and characterization of recombinant murine GALC (rm-GALC) from HEK293T cells, aiming to improve the feasibility of enzyme replacement therapy (ERT) for KD. An affinity chromatography protocol is refined to purify His-tagged rm-GALC, followed by buffer exchange and concentration steps to produce a stable and active enzyme suitable for subsequent in vitro applications. The purified rm-GALC is characterized for enzymatic activity, purity, and stability using SDS-PAGE, immunoblotting, and dynamic light scattering (DLS). In vitro assays reveal dose-dependent enzymatic activity recovery in KD primary cells upon rm-GALC administration, with no adverse effects on cell viability up to the physiological GALC dose. Additionally, GALC treatment at the physiological dose restored autophagic function in KD cells, as shown by LC3 and p62 marker analyses, confirming its compatibility with lysosomal-autophagic pathways. Conversely, supra-physiological GALC administration resulted in decreased viability and autophagy impairment. Finally, the feasibility of loading GALC into a polymeric nanovector based on stabilized reverse micelles is investigated. These findings highlight the critical importance of precise GALC dose regulation in developing a safe and effective enzyme replacement therapy (ERT) strategy for Krabbe disease (KD), further supporting the potential of a nanovector-mediated ERT approach.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal Microbiota Transplantation Modulates Th17/Treg Balance via JAK/STAT Pathway in ARDS Rats","authors":"Dongwei Zhang,&nbsp;Biying Dong,&nbsp;Jie Chen,&nbsp;Zhenqiang Zhang,&nbsp;Weitong Zeng,&nbsp;Longxiong Liao,&nbsp;Xia Xiong,&nbsp;Xuejun Qin,&nbsp;Xianming Fan","doi":"10.1002/adbi.202500028","DOIUrl":"10.1002/adbi.202500028","url":null,"abstract":"<p>This study evaluated the therapeutic effects of fecal microbiota transplantation (FMT) on lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) in rats. The study focused on the balance of T-helper 17 (Th17) and regulatory T (Treg) cells, as well as the modulation of the JAK/STAT pathway. This study established a rat ARDS model using intranasal LPS instillation, administering interventions such as FMT, Treg cell depletion, and JAK inhibitors. Assessments included histopathological examination of lung and intestinal tissues, flow cytometry for Th17 and Treg cell proportions, qPCR and Western blot for gene and protein expression, ELISA for inflammatory cytokines, and correlation analysis using Spearman's method for cytokine-immune cell interactions. Results indicated that FMT and JAK inhibitors significantly reduce lung damage induced by LPS, reduced alveolar destruction and inflammation, restored Th17/Treg balance, and inhibited JAK/STAT pathway activity. Notably, FMT decreased pro-inflammatory cytokines (IL-2, IL-6, IL-8, IL-17A, IL-23, TGF-β1) and increased anti-inflammatory cytokines (IL-10, IL-35) in serum. Spearman correlation analysis indicated that FMT restored immune balance by modulating the interactions between cytokines and immune cells. In conclusion, FMT effectively alleviates lung and intestinal injury in LPS-induced ARDS rat models by modulating Th17/Treg balance and inhibiting JAK/STAT pathway activity, demonstrating promising therapeutic potential for ARDS treatment.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202500028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting RAGE with Nanobodies for Molecular Imaging of Cancers and Alzheimer's Disease","authors":"Guangfeng Liang,&nbsp;Fujing Wang,&nbsp;Wei Xiong,&nbsp;Guangwei Shi,&nbsp;Junling Yuan,&nbsp;Yang Li,&nbsp;Hongyan Zhang,&nbsp;Yanmei Xing,&nbsp;Shan Jin,&nbsp;Kongjun Yang,&nbsp;Zhongliang Dai,&nbsp;Jichao Sun,&nbsp;Zhijie Li,&nbsp;Jianhong Wang","doi":"10.1002/adbi.202400617","DOIUrl":"10.1002/adbi.202400617","url":null,"abstract":"<p>The receptor for advanced glycation end products (RAGE) is a multifunctional cell surface receptor implicated in aging and the progression of chronic diseases, including cancer and Alzheimer's disease. Its interaction with advanced glycation end products (AGEs) promotes cellular stress and inflammation, underscoring the diagnostic and therapeutic relevance of targeting RAGE. In this study, we explored the potential of nanobodiessingle-domain antibodies known for high specificity, strong affinity, and deep tissue penetrationas molecular tools for RAGE-targeted applications. Using a phage display library, a panel of RAGE-specific nanobodies were isolated and characterized. Binding activity and affinity were evaluated through enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) assays. Among them, nanobody NbF8 demonstrated the highest affinity and specificity toward RAGE. In vitro, NbF8 selectively bound RAGE-expressing cells, while in vivo imaging in renal carcinoma and Alzheimer's disease mouse models confirmed its targeted accumulation in RAGE-overexpressing tumors and brain tissues. These findings highlight NbF8 as a promising molecular imaging agent for RAGE-associated diseases. This study supports the potential of RAGE-targeting nanobodies in both diagnostic imaging and therapeutic development, offering a novel approach for precision medicine in conditions driven by RAGE signaling.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400617","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lithocholic Acid, Calorie Restriction, and Halting Aging","authors":"Cade Ward,&nbsp;Michael M. Shahid,&nbsp;Grace Hohman,&nbsp;Mohamed A. Eldeeb","doi":"10.1002/adbi.202500110","DOIUrl":"10.1002/adbi.202500110","url":null,"abstract":"<p>While aging is a natural biological process, it is associated with a greater risk for multiple diseases, including cancer, neurodegeneration, and cardiovascular disease. Thus, it is important to study the biochemical mechanisms involved in aging to understand how to treat and prevent these health conditions. The discovery that calorie restriction (CR) promoted longevity in various organisms is a major breakthrough for aging research. Molecular studies of CR have revealed that it mediates its anti-aging effects by activating key signaling pathways, including the AMPK pathway. This pathway is important for regulating various processes, including energy homeostasis, metabolism, and proteostasis. Despite the advantages associated with CR, this practice can have detrimental effects, including decreased liver, body, and muscle mass. Additionally, CR is difficult to track and maintain, limiting its long-term potential. Interestingly, direct activation of the AMPK pathway offers a potential approach to increase longevity and quality of life without dietary restrictions. Remarkably, a recent discovery revealed that lithocholic acid (LCA), a metabolite from bile acid, could directly activate the AMPK pathway. Activation of the AMPK pathway by LCA leads to the beneficial effects of CR without the negative effects. These recent findings point to the possibility that supplementation of specific doses of LCA could offer a novel approach to induce anti-aging pathways that lead to increased longevity and improved quality of life.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision Medicine in ICH Unveiling the Superior Predictive Power of a Joint Model","authors":"Fa Wu,&nbsp;YuLin Yang,&nbsp;TingTing Wu,&nbsp;JinPing Sheng,&nbsp;FeiZhou Du,&nbsp;JianHao Li,&nbsp;ZhiWei Zuo,&nbsp;JunFeng Zhang,&nbsp;Rui Jiang,&nbsp;Peng Wang","doi":"10.1002/adbi.202400833","DOIUrl":"10.1002/adbi.202400833","url":null,"abstract":"<p>This study examined the effectiveness of a combined model using non-contrast computed tomography (NCCT) imaging, clinical data, and radiomics for predicting early hematoma enlargement in patients with spontaneous intracerebral hemorrhage. The study involved 232 patients with primary cerebral hemorrhage who met the inclusion criteria at the General Hospital of the Western Theater Command, PLA, between January 2018 and December 2023. Imaging and clinical features were compared, radiomic features were extracted from head CT scans, and a multivariate logistic regression model identified key imaging markers and clinical features. Univariate and multivariate logistic regression models were used for dimensionality reduction of radiomic features and to develop a radiomic signature/model. Patients were split into training and validation sets in a 7:3 ratio. Then, NCCT, clinical, radiomics, and combined NCCT-clinical-radiomics models were built, along with a nomogram. The AUC values for hematoma expansion prediction were as follows in the training set: NCCT model (0.758), clinical model (0.742), radiomics model (0.779), and combined model (0.872). In the validation set, the AUCs were: NCCT model (0.853), clinical model (0.754), radiomics model (0.778), and combined model (0.905). Calibration and decision curve analysis further confirmed the superior clinical utility of the combined model over the individual models. In conclusion, the combined NCCT-clinical-radiomics model significantly outperformed the individual models, leading to improved predictive accuracy, stability, and generalizability.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Approaches to Understand Movement as Medicine","authors":"Kieran F. Reid","doi":"10.1002/adbi.202400709","DOIUrl":"10.1002/adbi.202400709","url":null,"abstract":"&lt;p&gt;This &lt;i&gt;Special Section&lt;/i&gt; of &lt;i&gt;Advanced Biology&lt;/i&gt; provides new insights, novel perspectives, and future directions to advance our understanding of “movement as medicine”. With a distinct translational science perspective, this section included studies ranging from molecular level transcriptome experiments in mice to late phase efficacy clinical trials of mind-body interventions. A unique combination of original investigations is presented that describes two preclinical studies of exercise training to induce neural regeneration and cardiac remodeling,&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;1, 2&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; an innovative characterization of the gut microbiome within elite athletes and sedentary controls,&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;3&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; studies on new approaches to further delineate anthropometric aspects of sarcopenia&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;4&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; and sarcopenic obesity,&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;5&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; a characterization of neurohemodynamic responses to acute aerobic exercise in pre-dementia older adults,&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;6&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; the efficacy of a music-based mind-body program of Dalcroze Eurthymics for improving patient-important outcomes in older adults at high fall risk,&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;7&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; and a comprehensive narrative review of physical, pharmacological, and multimodality therapeutic approaches to mitigate the impact of musculoskeletal diseases among individuals living with spinal cord injury (SCI).&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;8&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt;&lt;/p&gt;&lt;p&gt;Fang et al.&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; sought to examine the potential molecular mechanisms of exercise-induced axonal regeneration in a mouse model of optic nerve injury. Several weeks of exercise stimulation restored DNA methylation patterns and promoted retinal ganglion cell (RGC) axon regeneration via TET3 mediated epigenetic effects. The authors then further demonstrated in a series of elegant experiments that exercise training induced RGC axon regeneration, reestablished visual circuits, partially restored vision loss, and improved metabolic function in older mice. This elucidation of the mechanistic effects of exercise-induced regeneration of these functionally important CNS neurons may allow for the further development of novel regenerative approaches to mitigate the adverse effects of optic neuropathy in humans. In another preclinical study, Han et al.&lt;sup&gt;[&lt;/sup&gt;&lt;span&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/span&gt;&lt;sup&gt;]&lt;/sup&gt; examined whether a combination of endurance and resistance training could improve cardiac function. Compared to sedentary controls, aging mice underwent an 8-week intensive and progressive swimming or voluntary resistance running training regimen. This experimental approach revealed that both swimming and voluntary resistance running attenuated age-related cardiac hypertrophy and cellular senescence, cardiac metabolism, and oxidative stress, and they improved ","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}