Advanced biology最新文献_第5页

The Impact of Decellularization Method on the Cytocompatibility and Wound Healing Capability of Human Amniotic Membrane 脱细胞法对人羊膜细胞相容性及伤口愈合能力的影响。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-17 DOI: 10.1002/adbi.202400509

Haadia Tauseef, Kainat Ahmed, Faiza Chaudhary, Asmat Salim, Omair Anwar Mohiuddin

{"title":"The Impact of Decellularization Method on the Cytocompatibility and Wound Healing Capability of Human Amniotic Membrane","authors":"Haadia Tauseef, Kainat Ahmed, Faiza Chaudhary, Asmat Salim, Omair Anwar Mohiuddin","doi":"10.1002/adbi.202400509","DOIUrl":"10.1002/adbi.202400509","url":null,"abstract":"The decellularized human amniotic membrane (dHAM) has been evaluated as a biomaterial for various tissue engineering applications, notably as a skin dressing for wound healing. The decellularization process alters the composition and structure of the extracellular matrix consequently influencing its characteristics. The aim of the present study was to comparatively evaluate dHAM-E and dHAM-S prepared by enzymatic and salt solution treatment respectively for their microstructure using scanning electron microscopy (SEM), in vitro biocompatibility with mesenchymal stem cells (MSCs), and regenerative capability in full-thickness wound model in mice. The SEM results revealed increased porosity in dHAM-S and better MSC adhesion and proliferation as compared to dHAM-E. Moreover, wound healing capability assessed at day 7 and day 14 by histological analysis of the regenerated tissues indicated that the dHAM treated groups achieved greater re-epithelialization and remodeling than the untreated group. However, dHAM-S treated samples presented a more remodeled regenerated skin than the other groups. Furthermore, gene expression analysis of the regenerated skin displayed a higher expression of anti-inflammatory, proliferation, and keratinization marker genes in the dHAM treated groups. Overall, it was found that dHAMs are compatible with MSCs and improve wound healing. However, clear differences were observed in the bioactivity of the two dHAMs.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging (Adv. Biology 2/2025) 黑素细胞和SK-MEL-28黑色素瘤细胞的数字全息显微镜和定量相成像的形态学和光学分析（ad . Biology 2/2025）

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-16 DOI: 10.1002/adbi.202570093

Ayah A. Farhat, Yazan A. Almahdi, Fatima Z. Alshuhani, Besa Xhabija

引用次数: 0

Vectors in CRISPR Gene Editing for Neurological Disorders: Challenges and Opportunities 神经系统疾病的CRISPR基因编辑载体：挑战与机遇。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-14 DOI: 10.1002/adbi.202400374

Kexin Xiong, Xiaxia Wang, Caicai Feng, Kaixiang Zhang, Di Chen, Sen Yang

{"title":"Vectors in CRISPR Gene Editing for Neurological Disorders: Challenges and Opportunities","authors":"Kexin Xiong, Xiaxia Wang, Caicai Feng, Kaixiang Zhang, Di Chen, Sen Yang","doi":"10.1002/adbi.202400374","DOIUrl":"10.1002/adbi.202400374","url":null,"abstract":"Diseases of the nervous system are recognized as the second leading cause of death worldwide. The global prevalence of neurological diseases, such as Huntington's disease, Alzheimer's disease, and Parkinson's disease has seen a significant rise due to the increasing proportion of the aging population. The discovery of the clustered regularly interspaced short palindromic repeats (CRISPR) genome editing technique has paved way for universal neurological diseases treatment. However, finding a safe and effective method to deliver CRISPR gene-editing tools remains a main challenge for genome editing therapies in vivo. Adeno-associated virus (AAV) is currently one of the most commonly used vector systems, but some issues remain unresolved, including capsid immunogenicity, off-target mutations, and potential genotoxicity. To address these concerns, researchers are actively encouraging the development of new delivery systems, like virus-like particles and nanoparticles. These novel systems have the potential to enhance targeting efficiency, thereby offering possible solutions to the current challenges. This article reviews CRISPR delivery vectors for neurological disorders treatment and explores potential solutions to overcome limitations in vector systems. Additionally, the delivery strategies of CRISPR systems are highlighted as valuable tools for studying neurological diseases, and the challenges and opportunities that these vectors present.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broadening the Healthy Aging Paradigm: Inclusion of Gestation, Development, and Reproductive Health 拓宽健康老龄化范式：包括妊娠、发育和生殖健康。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-14 DOI: 10.1002/adbi.202400481

Lan Yao, John Eikelboom, Andrew Macnab, Zhuo Yu, Yanhong Cao, Wei Wei, Shuqiu Sun, Asma Khalil, William C. Cho, Farah Naz Qamar, Peter Kremsner, Miquel Oliu-Barton, Lishi Wang, Ivan Sisa, Florian Marks, Cong-Yi Wang, J. Carolyn Graff, Scott C. Howard, Tianshu Gu, Marcello Maida, Edward V Prochownik, Tapfumanei Mashe, Alyce D Fly, Yang Hui, Andrea B. Maier, Sami Aleya, Dianjun Sun, Gang Wang, Evelyne Yehudit Bischof, Lotfi Aleya, Weikuan Gu

{"title":"Broadening the Healthy Aging Paradigm: Inclusion of Gestation, Development, and Reproductive Health","authors":"Lan Yao, John Eikelboom, Andrew Macnab, Zhuo Yu, Yanhong Cao, Wei Wei, Shuqiu Sun, Asma Khalil, William C. Cho, Farah Naz Qamar, Peter Kremsner, Miquel Oliu-Barton, Lishi Wang, Ivan Sisa, Florian Marks, Cong-Yi Wang, J. Carolyn Graff, Scott C. Howard, Tianshu Gu, Marcello Maida, Edward V Prochownik, Tapfumanei Mashe, Alyce D Fly, Yang Hui, Andrea B. Maier, Sami Aleya, Dianjun Sun, Gang Wang, Evelyne Yehudit Bischof, Lotfi Aleya, Weikuan Gu","doi":"10.1002/adbi.202400481","DOIUrl":"10.1002/adbi.202400481","url":null,"abstract":"The prevailing focus of lifespan health research has predominantly centered on “healthy aging”. This oversight may hinder the understanding of health across the lifespan, as disorders in earlier stages can substantially impact overall health and longevity. Aging, conceptually, begins at gestation. The trajectory of an individual's health is influenced from the earliest stages of life, where adverse conditions can set a foundation for lifelong health challenges. For example, suboptimal conditions during gestation leading to premature birth can predispose individuals to various health issues later in life. Additionally, precocious puberty defined as the onset of sexual maturity before eight years of age or early menopause—occurring before 50 years of age requires medical intervention and is indicative of atypical aging processes. To address these critical gaps in lifespan health research, the expansion of medical lexicons and research categorizations is advocated to include “healthy gestation,” “healthy development,” and “healthy reproduction” alongside “healthy aging.” This broader terminology will enable a more comprehensive investigation of disorders at all life stages. An integrative approach underscores the interconnectedness of all life stages and the continuous nature of aging, advocating for a seamless continuum in health research and interventions from gestation through late adulthood.\u0000","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Extrachromosomal DNA in Cancer: Biogenesis, Methodologies, Functions, and Therapeutic Potential 染色体外DNA在癌症中的作用：生物发生、方法、功能和治疗潜力。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-13 DOI: 10.1002/adbi.202400433

Xudong Mao, Guocheng Rao, Gonghui Li, Shihan Chen

{"title":"Insights into Extrachromosomal DNA in Cancer: Biogenesis, Methodologies, Functions, and Therapeutic Potential","authors":"Xudong Mao, Guocheng Rao, Gonghui Li, Shihan Chen","doi":"10.1002/adbi.202400433","DOIUrl":"10.1002/adbi.202400433","url":null,"abstract":"Originating from, but independent of, linear chromosomes, extrachromosomal DNA (ecDNA) exists in a more active state of transcription and autonomous replication. It plays a crucial role in the development of malignancies and therapy resistance. Since its discovery in eukaryotic cells more than half a century ago, the biological characteristics and functions of ecDNA have remained unclear due to limitations in detection methods. However, recent advancements in research tools have transformed ecDNA research. It is believed that ecDNA exhibits greater activity in the abnormal amplification of oncogenes, thereby driving cancer progression through their overexpression. Notably, compared to linear DNA, ecDNA can also function as a genomic element with regulatory roles, including both trans- and cis-acting functions. Its critical roles in tumorigenesis, evolution, progression, and drug resistance in malignant tumors are increasingly recognized. This review provides a comprehensive summary of the evolutionary context of ecDNA and highlights significant progress in understanding its biological functions and potential applications as a therapeutic target in malignant tumors.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Considering the Risks and Costs of Solid Organ Xenotransplantation 考虑实体器官异种移植的风险和成本。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-13 DOI: 10.1002/adbi.202400453

Catharine E. Krebs, Janine McCarthy, Kristie Sullivan, James Craner, Brendan Parent, Ann Lam

{"title":"Considering the Risks and Costs of Solid Organ Xenotransplantation","authors":"Catharine E. Krebs, Janine McCarthy, Kristie Sullivan, James Craner, Brendan Parent, Ann Lam","doi":"10.1002/adbi.202400453","DOIUrl":"10.1002/adbi.202400453","url":null,"abstract":"The standard treatment for end-stage organ failure is transplantation, but demand for organs has always vastly outstripped supply. Discussions are ongoing about the feasibility of addressing the organ shortage through measures like increasing organ donations, improving post-transplant outcomes, and xenotransplantation. This paper examines the rationale, risks, and costs of xenotransplantation, such as xenozoonoses, creating a new form of industrialized animal farming, abandoning animal ethics principles, and the opportunity costs of investing finite research dollars in xenotransplantation instead of investing in more viable strategies. Alternative strategies that can ethically and effectively address the demand for heart, kidney, and other transplants are recommended: Improving disease prevention and management to reduce demand for transplant organs, improving transplantation methods, and systemic changes to donor policies and organ recovery methods to increase overall supply. Upon careful exploration of the full landscape of organ transplantation, it is considered whether these alternative strategies that do not impose the definite harms and significant risks of xenotransplantation are the most ethical and effective means to increase life-saving options and improve clinical outcomes for patients in organ failure.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coacervates Composed of Low-Molecular-Weight Compounds– Molecular Design, Stimuli Responsiveness, Confined Reaction 由低分子量化合物组成的共渗物--分子设计、刺激响应性和封闭反应。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-12 DOI: 10.1002/adbi.202400572

Sayuri L. Higashi, Masato Ikeda

引用次数: 0

Mucus Physically Restricts Influenza A Viral Particle Access to the Epithelium 黏液物理上限制甲型流感病毒颗粒进入上皮。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-12 DOI: 10.1002/adbi.202400329

Logan Kaler, Elizabeth M. Engle, Maria Corkran, Ethan Iverson, Allison Boboltz, Maxinne A. Ignacio, Taj Yeruva, Margaret A. Scull, Gregg A. Duncan

{"title":"Mucus Physically Restricts Influenza A Viral Particle Access to the Epithelium","authors":"Logan Kaler, Elizabeth M. Engle, Maria Corkran, Ethan Iverson, Allison Boboltz, Maxinne A. Ignacio, Taj Yeruva, Margaret A. Scull, Gregg A. Duncan","doi":"10.1002/adbi.202400329","DOIUrl":"10.1002/adbi.202400329","url":null,"abstract":"Prior work suggests influenza A virus (IAV) crosses the airway mucus barrier in a sialic acid-dependent manner through the actions of the viral envelope proteins, hemagglutinin, and neuraminidase. However, host and viral factors that influence how efficiently mucus traps IAV remain poorly defined. In this work, how the physicochemical properties of mucus influence its ability to effectively capture IAV is assessed using fluorescence video microscopy and multiple particle tracking. Our studies suggest an airway mucus gel layer must be produced with virus-sized pores to physically constrain IAV. While sialic acid binding by IAV may improve mucus trapping efficiency, sialic acid binding preference is found to have little impact on IAV mobility and the fraction of viral particles expected to penetrate the mucus barrier. Further, synthetic polymeric hydrogels engineered with mucus-like architecture are similarly protective against IAV infection despite their lack of sialic acid decoy receptors. Together, this work provides new insights on mucus barrier function toward IAV with important implications on innate host defense and transmission of respiratory viruses.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202400329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology in Plant Nanobionics: Mechanisms, Applications, and Future Perspectives 植物纳米仿生学中的纳米技术：机制、应用和未来展望。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-12 DOI: 10.1002/adbi.202400589

Kajal Gautam, Hukum Singh, A. K. Sinha

{"title":"Nanotechnology in Plant Nanobionics: Mechanisms, Applications, and Future Perspectives","authors":"Kajal Gautam, Hukum Singh, A. K. Sinha","doi":"10.1002/adbi.202400589","DOIUrl":"10.1002/adbi.202400589","url":null,"abstract":"Plants are vital to ecosystems and human survival, possessing intricate internal and inter-plant signaling networks that allow them to adapt quickly to changing environments and maintain ecological balance. The integration of engineered nanomaterials (ENMs) with plant systems has led to the emergence of plant nanobionics, a field that holds the potential to enhance plant capabilities significantly. This integration may result in improved photosynthesis, increased nutrient uptake, and accelerated growth and development. Plants treated with ENMs can be stress mitigators, pollutant detectors, environmental sensors, and even light emitters. This review explores recent advancements in plant nanobionics, focusing on nanoparticle (NP) synthesis, adhesion, uptake, transport, fate, and application in enhancing plant physiological functioning, stress mitigation, plant health monitoring, energy production, environmental sensing, and overall plant growth and productivity. Potential research directions and challenges in plant nanobionics are highlighted, and how material optimization and innovation are propelling the growth in the field of smart agriculture, pollution remediation, and energy/biomass production are discussed.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"9 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetics and Its Application in Nervous System Diseases 光遗传学及其在神经系统疾病中的应用。

IF 3.2 3区生物学

Advanced biology Pub Date : 2025-02-10 DOI: 10.1002/adbi.202400416

Guocheng Mei, Huiqiong Pan, Hang Xu, Kepei Chen, Weihong Zheng, Hualin Xu, Yuetong Chen, Wei Lin, Jie Yang, Zhenlang Lin, Zhiming Liu, Min Zhang

引用次数: 0