Advanced biology最新文献_第5页

Accurate Identification of Cancer Cells in Complex Pre-Clinical Models Using a Deep-Learning Neural Network: A Transfection-Free Approach (Adv. Biology 11/2024) 利用深度学习神经网络准确识别复杂临床前模型中的癌细胞：无转染方法（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470112

Marilisa Cortesi, Dongli Liu, Elyse Powell, Ellen Barlow, Kristina Warton, Caroline E. Ford

引用次数: 0

Masthead: (Adv. Biology 11/2024) 刊头：（Adv. Biology 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470113

引用次数: 0

Predicting Clinical Outcomes of SARS-CoV-2 Drug Efficacy with a High-Throughput Human Airway Microphysiological System (Adv. Biology 11/2024) 利用高通量人体气道微生理系统预测SARS-CoV-2药物疗效的临床结果（生物学进展 11/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-12 DOI: 10.1002/adbi.202470111

Landys Lopez Quezada, Felix Mba Medie, Rebeccah J. Luu, Robert B. Gaibler, Elizabeth P. Gabriel, Logan D. Rubio, Thomas J. Mulhern, Elizabeth E. Marr, Jeffrey T. Borenstein, Christine R. Fisher, Ashley L. Gard

引用次数: 0

Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging. 通过数字全息显微镜和定量相位成像对黑色素细胞和 SK-MEL-28 黑色素瘤细胞进行形态学和光学分析。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-11-11 DOI: 10.1002/adbi.202400346

Ayah A Farhat, Yazan A Almahdi, Fatima Z Alshuhani, Besa Xhabija

{"title":"Morphological and Optical Profiling of Melanocytes and SK-MEL-28 Melanoma Cells Via Digital Holographic Microscopy and Quantitative Phase Imaging.","authors":"Ayah A Farhat, Yazan A Almahdi, Fatima Z Alshuhani, Besa Xhabija","doi":"10.1002/adbi.202400346","DOIUrl":"https://doi.org/10.1002/adbi.202400346","url":null,"abstract":"Melanoma, which originates from pigment-producing melanocytes, is an aggressive and deadly skin cancer. Despite extensive research, its mechanisms of progression and metastasis remain unclear. This study uses quantitative phase imaging via digital holographic microscopy, Principal Component Analysis (PCA), and t-distributed Stochastic Neighbor Embedding (t-SNE) to identify the morphological, optical, and behavioral differences between normal melanocytes and SK-MEL-28 melanoma cells. Our findings reveal significant differences in cell shape, size, and internal organization, with SK-MEL-28 cells displaying greater size variability, more polygonal shapes, and higher optical thickness. Phase shift parameters and surface roughness analyses underscore melanoma cells' uniform and predictable textures. Violin plots highlight the dynamic and varied migration of SK-MEL-28 cells, contrasting with the localized movement of melanocytes. Hierarchical clustering of correlation matrices provides further insights into complex morphological and optical relationships. Integrating label-free imaging with robust analytical methods enhances understanding of melanoma's aggressive behavior, supporting targeted therapies and highlighting potential biomarkers for precise melanoma diagnostics and treatment.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400346"},"PeriodicalIF":3.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Update on Theoretical and Metrological Aspects of the Surface Hydrophobicity of Virus and Virus-Like Particles. 病毒和类病毒颗粒表面疏水性的理论和计量方面的最新情况。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-22 DOI: 10.1002/adbi.202400221

Guillaume Sautrey

{"title":"An Update on Theoretical and Metrological Aspects of the Surface Hydrophobicity of Virus and Virus-Like Particles.","authors":"Guillaume Sautrey","doi":"10.1002/adbi.202400221","DOIUrl":"https://doi.org/10.1002/adbi.202400221","url":null,"abstract":"Viruses are biological entities embodied in protein-based nanoparticles devoid of metabolic activity. Hence, the colloidal, interfacial, and chemical reactivity of virus particles (VPs) profoundly affects the fate of natural and artificial viruses in biotic or abiotic aqueous systems. These rely on the physical chemistry at the outer surface of VPs. In other words, whether wild or synthetic VPs and regardless of the scientific fields involved, taming viruses implies thus managing the physical chemistry at the VP external surface. The surface hydrophobicity (SH) of VPs is a critical feature that must be looked at. Still, the literature dealing with nanoscale hydrophobic domains at the proteinaceous surface of VPs underlying their global SH is like a fragmented puzzle. This article provides an overview of the topic from the perspective of modern protein biophysics for updating the classic physicochemical picture of outer VP/water interfaces hitherto accepted. Patterns of non-polar and \"false-polar\" patches, expressing variable hydrophobic degrees according to neighboring polar patches, are now drawn. The extensive discussion of reviewed data generates such fresh ideas to explore in the coming years for better modeling the SH of wild virions or engineered virus-based nanoparticles, paving the way for new directions in fundamental virology and virus-based chemistry.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400221"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the Supramolecular Interaction of Positively Supercharged Fluorescent Protein with Anionic Phthalocyanines. 阐明带正电荷的荧光蛋白与阴离子酞菁的超分子相互作用。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-16 DOI: 10.1002/adbi.202400308

Sharon Saarinen, Ramsha Khan, Marta Patrian, Juan Pablo Fuenzalida-Werner, Rubén D Costa, Petr Zimcik, Veronika Novakova, Tero-Petri Ruoko, Nikolai V Tkachenko, Eduardo Anaya-Plaza, Mauri A Kostiainen

{"title":"Elucidating the Supramolecular Interaction of Positively Supercharged Fluorescent Protein with Anionic Phthalocyanines.","authors":"Sharon Saarinen, Ramsha Khan, Marta Patrian, Juan Pablo Fuenzalida-Werner, Rubén D Costa, Petr Zimcik, Veronika Novakova, Tero-Petri Ruoko, Nikolai V Tkachenko, Eduardo Anaya-Plaza, Mauri A Kostiainen","doi":"10.1002/adbi.202400308","DOIUrl":"https://doi.org/10.1002/adbi.202400308","url":null,"abstract":"Developing bioinspired materials to convert sunlight into electricity efficiently is paramount for sustainable energy production. Fluorescent proteins are promising candidates as photoactive materials due to their high fluorescence quantum yield and absorption extinction coefficients in aqueous media. However, developing artificial bioinspired photosynthetic systems requires a detailed understanding of molecular interactions and energy transfer mechanisms in the required operating conditions. Here, the supramolecular self-assembly and photophysical properties of fluorescent proteins complexed with organic dyes are investigated in aqueous media. Supercharged mGreenLantern protein, mutated to have a charge of +22, is complexed together with anionic zinc phthalocyanines having 4 or 16 carboxylate groups. The structural characterization reveals a strong electrostatic interaction between the moieties, accompanied by partial conformational distortion of the protein structure, yet without compromising the mGreenLantern chromophore integrity as suggested by the lack of emission features related to the neutral form of the chromophore. The self-assembled biohybrid shows a total quenching of protein fluorescence, in favor of an energy transfer process from the protein to the phthalocyanine, as demonstrated by fluorescence lifetime and ultrafast transient absorption measurements. These results provide insight into the rich photophysics of fluorescent protein-dye complexes, anticipating their applicability as water-based photoactive materials.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400308"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Proteomics Approaches for Screening Small Molecule Inhibitors Covalently Binding to SARS-Cov-2 筛选与 SARS-Cov-2 共价结合的小分子抑制剂的化学蛋白质组学方法。

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-15 DOI: 10.1002/adbi.202300612

Liuhai Zheng, Qian Zhang, Piao Luo, Fei Shi, Ying Zhang, Xiaoxue He, Yehai An, Guangqing Cheng, Xiaoyan Pan, Zhijie Li, Boping Zhou, Jigang Wang

{"title":"Chemical Proteomics Approaches for Screening Small Molecule Inhibitors Covalently Binding to SARS-Cov-2","authors":"Liuhai Zheng, Qian Zhang, Piao Luo, Fei Shi, Ying Zhang, Xiaoxue He, Yehai An, Guangqing Cheng, Xiaoyan Pan, Zhijie Li, Boping Zhou, Jigang Wang","doi":"10.1002/adbi.202300612","DOIUrl":"10.1002/adbi.202300612","url":null,"abstract":"Although various strategies have been used to prevent and treat SARS-CoV-2, the spread and evolution of SARS-CoV-2 is still progressing rapidly. The emerging variants Omicron and its sublineage have a greater ability to spread and escape nearly all current monoclonal antibodies treatments, highlighting an urgent need to develop therapeutics targeting current and emerging Omicron variants or recombinants with breadth and potency. Here, some small molecule drugs are rapidly identified that could covalently binding to receptor binding domain (RBD) protein of Omicron through the combined application of artificial intelligence (AI) and activity-based protein profiling (ABPP) technology. The surface plasmon resonance (SPR) and pseudo-virus neutralization experiments further reveal that an FDA-approved drug gallic acid has robust neutralization potency against Omicron pseudo-virus with the IC50 values of 23.56 × 10−6 m. Taken together, a platform combining AI intelligence, biochemical, SPR, molecular docking, and pseudo-virus-based screening for rapid identification and evaluation of potential anti-SARS-CoV-2 small molecule drugs is established and the effectiveness of the platform is validated.","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"8 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: (Adv. Biology 10/2024) 刊头：（Adv. Biology 10/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-13 DOI: 10.1002/adbi.202470102

引用次数: 0

Delivery of TGFβ3 from Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro (Adv. Biology 10/2024) 磁响应同轴纤维传递 TGFβ3 可降低体外脊髓星形胶质细胞的反应性（生物学进展 10/2024）

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-13 DOI: 10.1002/adbi.202470101

Jessica L. Funnell, Jasper Fougere, Diana Zahn, Silvio Dutz, Ryan J. Gilbert

{"title":"Delivery of TGFβ3 from Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro (Adv. Biology 10/2024)","authors":"Jessica L. Funnell, Jasper Fougere, Diana Zahn, Silvio Dutz, Ryan J. Gilbert","doi":"10.1002/adbi.202470101","DOIUrl":"https://doi.org/10.1002/adbi.202470101","url":null,"abstract":"Drug DeliverySpinal cord injury (SCI) is a devastating condition that severely impacts patient quality of life, and there are no available treatments that restore lost function. Biomaterials can provide local, sustained release of therapeutics, but drug-releasing biomaterials do not address variability in injury severity. To tune delivery to a unique injury, Ryan J. Gilbert and co-workers developed a fibrous scaffold that can be stimulated with a magnetic field to alter the release rate of a growth factor. The authors found that sustained release of the growth factor resulted in a greater reduction of spinal cord astrocyte reactivity compared to bolus delivery in vitro. The astrocytes treated with the drug-releasing scaffold supported sensory neuron growth in coculture, shown in the flourescence image. Article number 2300531 provides a foundation for developing biomaterials capable of tunable growth factor release in response to externally applied magnetic fields for SCI treatment.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":"8 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adbi.202470101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Idiopathic Pulmonary Fibrosis Caused by Damaged Mitochondria and Imbalanced Protein Homeostasis in Alveolar Epithelial Type II Cell. 肺泡上皮 II 型细胞线粒体受损和蛋白质平衡失调导致特发性肺纤维化

IF 3.2 3区生物学

Advanced biology Pub Date : 2024-10-10 DOI: 10.1002/adbi.202400297

Zhaoxiong Dong, Xiaolong Wang, Peiwen Wang, Mingjian Bai, Tianyu Wang, Yanhui Chu, Yan Qin

引用次数: 0