Forskolin增强人脂肪组织源性干细胞无xeno培养的尿激酶纤溶酶原激活物分泌和血管生成活性。

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Maria Vittoria Giraudo, Anne Therese Lauvrud, Rebecca Wiberg, Maria Brohlin, Gustav Andersson, Paul J Kingham
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引用次数: 0

摘要

脂肪组织源性干细胞(ASCs)在治疗各种临床疾病方面具有巨大的潜力。为了增强其再生特性,可以使用各种体外方案对ASCs进行化学刺激。然而,令人不满意的结果仍然存在,部分原因是相对昂贵的长期方法。此外,目前的培养技术往往依赖于异种胎牛血清的使用,这可能是免疫原性的,限制了临床转化。为了促进ASCs衍生疗法的临床翻译,研究了不同刺激方案对无xeno培养基(PRIME-XV MSC Expansion XSFM)中培养的人ASCs的影响。在无xeno培养基中添加刺激物(forskolin (FSK),碱性成纤维细胞生长因子,血小板衍生生长因子- aa,神经调节因子-1)联合或单独。FSK单独刺激72小时,或与生长因子一起刺激72小时,可增强尿激酶纤溶酶原激活剂(uPA)的产生,uPA是一种参与组织重塑过程的丝氨酸蛋白酶。由受刺激的ASCs衍生的条件培养基增强了体外血管生成和内皮细胞迁移。这项研究表明,使用无xeno培养基的短时间刺激方案可以增强人类ASCs的促血管生成反应。该方案使用现成的制造细胞治疗级分子,可能会提高ASCs分泌组的再生特性,从而提高其在临床治疗中的疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Forskolin Enhances Urokinase Plasminogen Activator Secretion and Angiogenic Activity of Xeno-Free Cultures of Human Adipose Tissue-Derived Stem Cells.

Adipose tissue-derived stem cells (ASCs) hold significant potential for treating various clinical conditions. To enhance their regenerative properties, ASCs can be chemically stimulated using various in vitro protocols. However, unsatisfactory results persist, partly due to the relatively costly long-term methods. Furthermore, current culturing techniques often rely on the use of xenogenic fetal bovine serum that can be immunogenic, limiting clinical translations. To facilitate clinical translation of ASCs-derived therapeutics, the effect of different stimulation protocols on human ASCs cultured in a xeno-free medium (PRIME-XV MSC Expansion XSFM) is investigated. The xeno-free medium was supplemented with stimulants (forskolin (FSK), basic fibroblast growth factor, platelet-derived growth factor-AA, neuregulin-1) in combinations or individually. Stimulation for 72 h in FSK alone, or together with the growth factors, enhanced the production of urokinase plasminogen activator (uPA), a serine protease involved in tissue remodeling processes. Conditioned medium derived from stimulated ASCs enhanced in vitro angiogenesis and endothelial cells migration. This study shows that pro-angiogenic responses in human ASCs can be enhanced with a defined short stimulation protocol using a xeno-free medium. The protocol, using readily available manufacturing cell therapy grade molecules, may boost the regenerative properties of ASCs secretome which could enhance their efficacy in clinical treatments.

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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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