双链DNA减少唾液中dsRNA降解并显著增强rnai介导的Halyomorpha halys基因沉默

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Venkata Partha Sarathi Amineni, Georg Petschenka, Aline Koch
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引用次数: 0

摘要

入侵害虫Halyomorpha halys(半翅目:蝽科)对农业造成重大威胁,需要化学农药以外的防治方法。本研究探讨了RNA干扰(RNAi)作为一种靶向基因沉默方法来管理halys种群。然而,RNAi的功效因昆虫目(包括半翅目昆虫)的不同而不同,这是由于存在于halys唾液中的DNA/RNA非特异性核酸酶(HhNSE)对双链RNA (dsRNA)的快速降解等因素。值得注意的是,本研究证明双链DNA (dsDNA)可以稳定唾液中的dsRNA,可能是通过竞争性抑制HhNSE, HhNSE在唾液腺中高度表达。针对网蛋白重链基因(HhCHC)的体内试验表明,与单独使用dsRNA相比,将dsRNA- chc和dsDNA的混合物喂给海螺时,基因沉默增强。虽然dsRNA-CHC注射剂导致几乎完全死亡,但与dsRNA-CHC一起喂养时,dsDNA制剂不会显著增加死亡率。这些发现强调需要进一步研究核酸酶以外的因素,如昆虫肠道中dsRNA的摄取和释放机制。尽管如此,这项研究为改善在halys和其他具有这种核酸酶的害虫中的RNAi传递提供了有希望的见解,以支持可持续的害虫管理解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys

Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys

Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys

Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys

Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys

The invasive pest Halyomorpha halys (Hemiptera: Pentatomidae) poses a significant threat to agriculture and requires control methods beyond chemical pesticides. This study investigates RNA interference (RNAi) as a targeted gene silencing approach to manage H. halys populations. However, RNAi efficacy varies between insect orders, including hemipterans, due to factors such as the rapid degradation of double-stranded RNA (dsRNA) by a DNA/RNA non-specific nuclease (HhNSE) present in the saliva of H. halys. Notably, this study proves that double-stranded DNA (dsDNA) can stabilise dsRNA in saliva, probably by competitively inhibiting HhNSE, which is highly expressed in salivary glands. In vivo tests targeting the clathrin heavy chain gene (HhCHC) demonstrate that a mixture of dsRNA-CHC and dsDNA result in enhanced gene silencing when fed to H. halys, compared to dsRNA alone. While dsRNA-CHC injection causes almost complete mortality, the dsDNA formulation do not significantly increase mortality when fed together with dsRNA-CHC. These findings highlight the need to further investigate factors beyond nucleases such as dsRNA uptake and release mechanisms in the insect gut. Nevertheless, this study provides promising insights for improving RNAi delivery in H. halys, and perhaps other pests with such nucleases, in support of sustainable pest management solutions.

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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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