Cancer Microenvironment-Stimulated Mesenchymal Stem Cells in an Indirect Co-Culture System Influence Cancer Cell Growth and Apoptosis.

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Pragati Sharma, Jun Nakanishi, Subha Narayan Rath
{"title":"Cancer Microenvironment-Stimulated Mesenchymal Stem Cells in an Indirect Co-Culture System Influence Cancer Cell Growth and Apoptosis.","authors":"Pragati Sharma, Jun Nakanishi, Subha Narayan Rath","doi":"10.1002/adbi.202500291","DOIUrl":null,"url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) migrate to injured tissues, aiding tissue repair, remodeling, and wound healing. As tumors are often considered to have traits of \"injured tissues,\" MSCs are recruited to tumor microenvironments where they can have pro- and antitumorigenic influences. This study assesses whether human mesenchymal stem cells (hMSCs) of shared ancestry exhibit similar tumorigenic properties. Bone marrow-derived (hBM-MSCs) and umbilical cord-derived (hUC-MSCs) MSCs embedded in collagen are cultured in conditioned media from lung adenocarcinoma (A549) cells to mimic the extracellular matrix and soluble cues of the cancer microenvironment. Cell viability, proliferation, and immunofluorescence analyses evaluate MSC behavior under these conditions. Further, A549 cells are exposed to conditioned media from cancer-stimulated MSCs to simulate indirect co-culture, and their response is assessed through viability, immunofluorescence, and flow cytometric analysis. Results show increased viability and proliferation of hBM-MSCs, morphological changes, and elevated alpha-smooth muscle actin expression, suggesting a transition toward cancer-associated fibroblasts. In contrast, hUC-MSCs display reduced viability and no morphological alterations. Conditioned media from cancer-exposed hUC-MSCs induce apoptosis in A549 cells, whereas hBM-MSCs support A549 growth. These findings demonstrate that, despite their common origin, hUC-MSCs and hBM-MSCs exhibit opposing responses to tumor cues and influence lung cancer cell behavior differently.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e00291"},"PeriodicalIF":2.6000,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/adbi.202500291","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Mesenchymal stem cells (MSCs) migrate to injured tissues, aiding tissue repair, remodeling, and wound healing. As tumors are often considered to have traits of "injured tissues," MSCs are recruited to tumor microenvironments where they can have pro- and antitumorigenic influences. This study assesses whether human mesenchymal stem cells (hMSCs) of shared ancestry exhibit similar tumorigenic properties. Bone marrow-derived (hBM-MSCs) and umbilical cord-derived (hUC-MSCs) MSCs embedded in collagen are cultured in conditioned media from lung adenocarcinoma (A549) cells to mimic the extracellular matrix and soluble cues of the cancer microenvironment. Cell viability, proliferation, and immunofluorescence analyses evaluate MSC behavior under these conditions. Further, A549 cells are exposed to conditioned media from cancer-stimulated MSCs to simulate indirect co-culture, and their response is assessed through viability, immunofluorescence, and flow cytometric analysis. Results show increased viability and proliferation of hBM-MSCs, morphological changes, and elevated alpha-smooth muscle actin expression, suggesting a transition toward cancer-associated fibroblasts. In contrast, hUC-MSCs display reduced viability and no morphological alterations. Conditioned media from cancer-exposed hUC-MSCs induce apoptosis in A549 cells, whereas hBM-MSCs support A549 growth. These findings demonstrate that, despite their common origin, hUC-MSCs and hBM-MSCs exhibit opposing responses to tumor cues and influence lung cancer cell behavior differently.

肿瘤微环境刺激间充质干细胞间接共培养系统对癌细胞生长和凋亡的影响。
间充质干细胞(MSCs)迁移到损伤组织,帮助组织修复、重塑和伤口愈合。由于肿瘤通常被认为具有“损伤组织”的特征,MSCs被招募到肿瘤微环境中,在那里它们可以具有促肿瘤和抗肿瘤的影响。本研究评估了具有共同祖先的人间充质干细胞(hMSCs)是否表现出相似的致瘤特性。骨髓来源(hBM-MSCs)和脐带来源(hUC-MSCs)的MSCs包埋在胶原中,在肺腺癌(A549)细胞的条件培养基中培养,以模拟细胞外基质和癌症微环境的可溶性线索。细胞活力,增殖和免疫荧光分析评估MSC在这些条件下的行为。此外,将A549细胞暴露于来自癌症刺激的MSCs的条件培养基中以模拟间接共培养,并通过活力、免疫荧光和流式细胞术分析评估其反应。结果显示hBM-MSCs的活力和增殖增加,形态学改变,α -平滑肌肌动蛋白表达升高,表明向癌症相关成纤维细胞过渡。相比之下,hUC-MSCs表现出活力降低,没有形态学改变。来自癌症暴露的hUC-MSCs的条件培养基诱导A549细胞凋亡,而hBM-MSCs支持A549细胞的生长。这些发现表明,尽管来源相同,hUC-MSCs和hBM-MSCs对肿瘤信号表现出相反的反应,并对肺癌细胞行为产生不同的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信