Marine Biotechnology最新文献

{"title":"Epigallocatechin-3-gallate Attenuates the Bromo-3-chloro-5,5-dimethylhydantoin-induced Immunotoxicity in Crayfish","authors":"Yong Wang,&nbsp;Yinglei Xu,&nbsp;Fei Zhu","doi":"10.1007/s10126-025-10449-6","DOIUrl":"10.1007/s10126-025-10449-6","url":null,"abstract":"<div><p>Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH), a widely used environmental disinfectant in aquaculture, may induce toxicity, adversely affecting the health and viability of aquatic organisms. Epigallocatechin-3-gallate (EGCG), a polyphenol present in green tea, exhibits antioxidant properties that can protect normal cells from oxidative stress. The findings suggest that exposure to BCDMH results in a reduction of antioxidant enzyme activity, whereas EGCG supplementation enhances crayfish immunity and alleviates damage. Moreover, BCDMH exposure is associated with a decrease in total hemocyte count and an increase in apoptosis rate; however, EGCG demonstrates a protective effect against BCDMH-induced cytotoxicity. Histopathological analysis indicates that exposure to BCDMH results in hepatopancreatic damage in crayfish, which is mitigated by EGCG. To identify the genes and pathways influenced by EGCG, a comparative transcriptome analysis was performed. Gene Ontology (GO) analysis revealed that proteolysis and innate immune response are significant biological processes induced by EGCG. Furthermore, KEGG pathway analysis identified endocytosis and phagosome as critical pathways modulated by EGCG. EGCG effectively enhanced the survival of crayfish challenged with <i>V. alginolyticus</i> following BCDMH exposure This study contributes to fully understand the mechanisms of EGCG in reducing the immunotoxicity of antibiotic residues on aquatic animals.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Metabolomic Analyses Reveal Response Mechanisms of Sinonovacula Constricta to Saline-Alkalinity Stresses","authors":"Min Yang,&nbsp;Yuting Han,&nbsp;Yujie Chang,&nbsp;Chengbo Li,&nbsp;Donghong Niu","doi":"10.1007/s10126-025-10445-w","DOIUrl":"10.1007/s10126-025-10445-w","url":null,"abstract":"<div><p>The razor clam (<i>Sinonovacula constricta</i>) is a key species in marine aquaculture, known for its wide salinity adaptation, and potential for cultivation in saline-alkaline water. Understanding its response mechanisms is crucial for expanding its farming into these regions. This study reveals the response mechanisms of <i>S. constricta</i> in response to low-salinity alkaline stress through a combined analysis of transcriptomics and metabolomics. After 24 h of salt-alkali stress (SA group), 1378 differentially expressed genes (DEGs) were identified, with enriched pathways including glycerophospholipid metabolism, serine, taurine, and hypotaurine metabolism. Additionally, 341 significantly different metabolites (SDMs) were found, primarily involved in taurine and hypotaurine metabolism, purine metabolism, and the FoxO signaling pathway, etc. Both DEGs and SDMs were notably enriched in hypotaurine metabolism, glycerophospholipid metabolism, and the mTOR signaling pathway, showing significant upregulation in the SA group. Correlation analysis found that the integrated regulatory network was involved in the synthesis of taurine, glycerophospholipids, and L-glutamic acid, and the metabolism of 3-mercaptopropionic acid. These results suggest that low salinity and alkalinity induce stress responses in <i>S. constricta</i> by regulating osmotic balance, phospholipid synthesis, and lipid metabolism. This study offers insights into the molecular mechanisms of salt-alkali response in <i>S. constricta</i>. </p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Plastid Acetyl-CoA Carboxylase Confers Stress Tolerances with Increased Levels of Unsaturated Fatty Acids in the Marine Diatom Phaeodactylum tricornutum","authors":"Masakazu Toyoshima,&nbsp;Hajime Okuda,&nbsp;Yuya Okada,&nbsp;Kohei Yoneda,&nbsp;Ginga Shimakawa,&nbsp;Yusuke Matsuda","doi":"10.1007/s10126-025-10446-9","DOIUrl":"10.1007/s10126-025-10446-9","url":null,"abstract":"<div><p>Acetyl-coenzyme A carboxylases (ACCs) catalyze the initial reaction of fatty acid (FA) biosynthesis. The marine diatom <i>Phaeodactylum tricornutum</i> has two nuclear-encoded ACCs (PtACC1 (Phatr3_EG01955) and PtACC2 (Phatr3_J55209)), both which are homomeric and predicted to be localized in the plastids and the cytosol, respectively. In this study, we focused on stromal ACC1 by constructing <i>P. tricornutum</i> strains expressing GFP-tagged PtACC1 (ACCG strains) and confirmed that PtACC1 was localized in or around the pyrenoid. Here, we showed that unsaturated FAs (UFAs) composing the thylakoid membrane lipids increased in PtACC1 strains grown under high light conditions (190 µmol photons m⁻² s⁻¹), and that the content of triacylglycerol (TAG) and unsaturation ratios in TAG increased under oxidative stresses (with added 50 µM H₂O₂). ACCG strains showed faster growth rates than wild type under high light and/or oxidative stress conditions. These results suggest that cell proliferation is maintained by an accelerated recovery of PSII due to the increased UFAs in the thylakoid membrane in ACCG strains grown in high light, and that increased UFAs in ACCG cells enhanced the tolerance to oxidative stresses presumably due to the increased scavenging capacity of UFAs against reactive oxygen species. The introduction of plastidic ACC resulted in stimulating supply of UFAs to specific lipids that in turn enhanced tolerance to various stresses.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10446-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals Differential Gene Regulation in Ovarian Tissues of Anabas testudineus (Bloch, 1792) in Response to Insecticide, Monocrotophos","authors":"Siyag A. Dhere,&nbsp;Pokanti Vinay Kumar,&nbsp;Kiran D. Rasal,&nbsp;Sujata Mohapatra,&nbsp;Prachi Asgolkar,&nbsp;Manohar Vasam,&nbsp;Sheetal Vanjre,&nbsp;Priyanka S. S.,&nbsp;Manoj P. Brahmane,&nbsp;Arvind Sonwane,&nbsp;Rajesh Kumar,&nbsp;Naresh S. Nagpure,&nbsp;Mukunda Goswami,&nbsp;Jitendra Sundaray","doi":"10.1007/s10126-025-10443-y","DOIUrl":"10.1007/s10126-025-10443-y","url":null,"abstract":"<div><p>Monocrotophos (MCP), an organophosphate insecticide commonly used in agriculture, has raised concerns due to its runoff into aquatic ecosystems and causes potential adverse effects on fish. The present study envisaged the understanding of the impact of MCP on the ovarian tissues of <i>Anabas testudineus</i> (climbing perch), an air-breathing food fish often found close to agricultural fields, making it a valuable bio-indicator of agrochemical contamination. Transcriptome profiling of ovarian tissues in response to 45 days of MCP exposure at sub-lethal concentrations was performed. Using Illumina platform sequencing, a total of 144.51 million reads were produced. After filtering and trimming, 138.82 million high-quality reads were obtained, of which 96.10% were mapped to the Anabas genome. Expression analysis revealed a total of 54 significant differentially expressed genes (DEGs), including 28 upregulated genes, and 26 downregulated genes compared to the control group (Log2 FC &gt;  ± 1 and, adjusted <i>p-value</i> &lt; 0.05). Gene ontology analysis of the DEGs revealed associations with molecular, biological, and cellular functions. Key detoxification genes, such as <i>glutathione S-transferase</i> and <i>UDP-glucuronosyltransferase</i>, were significantly upregulated, indicating an enhanced detoxification response to MCP. In contrast, <i>cytochrome P450 family 1 subfamily A (cyp1a1)</i>, a gene critical for steroid hormone metabolism, was downregulated, suggesting disruptions in hormone regulation. Functional enrichment analysis highlighted several affected processes, including steroid hormone biosynthesis, oocyte meiosis, apoptosis, and progesterone-mediated oocyte maturation. The randomly selected eight DEGs using RT-qPCR confirmed consistent gene expression levels in line with the transcriptome data. This work identified significant genes associated with detoxification and reproduction events in the ovarian tissues for maintaining homeostasis. This will also serve as valuable information for further investigation of the association of the identified genes with the reproductive biology of fish in response to toxicants or pollutants.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Supplements Significantly Improve the Growth Rate of Cultured Asparagopsis armata","authors":"Jiasui Li,&nbsp;Lucien Alperstein,&nbsp;Masayuki Tatsumi,&nbsp;Rocky de Nys,&nbsp;Jadranka Nappi,&nbsp;Suhelen Egan","doi":"10.1007/s10126-025-10440-1","DOIUrl":"10.1007/s10126-025-10440-1","url":null,"abstract":"<div><p>Seaweed aquaculture is an expanding industry with innovative applications beyond the traditional uses as human foods and phycocolloids. <i>Asparagopsis armata</i>, a red seaweed, is cultivated as a feed supplement to reduce methane emission from ruminants. The manipulation of microbiota with seaweed beneficial microorganisms (SBMs) has shown promise in enhancing disease resistance and growth in seaweeds and has potential to aid the cultivation of <i>A. armata</i>. In this study, we developed a growth assay for the rapid selection of bacteria that promote the growth of <i>A. armata</i> tetrasporophytes. We tested bacterial strains from the genera <i>Phaeobacter</i> and <i>Pseudoalteromonas</i> for their impact on the growth of <i>A. armata</i>, as these bacteria have been recognized for their beneficial traits in other seaweeds. All strains significantly enhanced the specific growth rate (SGR) of <i>A. armata</i> tetrasporophytes compared to controls without bacterial treatment. Bacterial 16S rRNA gene amplicon sequencing confirmed the presence of the inoculated growth-promoting SBMs (SBM-Gs) in <i>A. armata</i> cultures with no significant impacts on the resident microbial community. Co-occurrence network analysis of the resulting communities demonstrated that the inoculated <i>Phaeobacter</i> spp. formed distinct modules, exclusively interacting with resident <i>Phaeobacter</i> species, while the <i>Pseudoalteromonas</i> sp. was absent from the network. These results demonstrate that microbial inoculation is an effective strategy for incorporating SBM-Gs into the <i>A. armata</i> microbiota to promote growth. The tested SBM-Gs may exert their influence by interacting with specific resident species or by directly affecting host physiology, resulting in minimal undesired effects on the microbiome.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10440-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dielectrophoresis for Isolating Low-Abundance Bacteria Obscured by Impurities in Environmental Samples","authors":"Jaeyoung Yu,&nbsp;Hajime Yuasa,&nbsp;Ikuo Hirono,&nbsp;Keiichiro Koiwai,&nbsp;Tetsushi Mori","doi":"10.1007/s10126-025-10441-0","DOIUrl":"10.1007/s10126-025-10441-0","url":null,"abstract":"<div><p>Bacterial communities associated with living organisms play critical roles in maintaining health and ecological balance. While dominant bacteria have been widely studied, understanding the role of low-abundance bacteria has become increasingly important due to their unique roles, such as regulating bacterial community dynamics and supporting host-specific functions. However, detecting these bacteria remains challenging, as impurities in environmental samples mask signals and compromise the accuracy of analyses. This study explored the use of dielectrophoresis (DEP) as a practical approach to isolate low-abundance bacteria obscured by impurities, comparing its utility to conventional centrifugation methods. Using two shrimp species, <i>Neocaridina denticulata</i> and <i>Penaeus japonicus</i>, DEP effectively isolated bacterial fractions while reducing impurities, enabling the detection of bacteria undetected in centrifuged samples. These newly detected bacteria were potentially linked to diverse ecological and host-specific functions, such as nutrient cycling and immune modulation, highlighting DEP as a highly potential approach to support the study of host-microbial interactions. Overall, we believe that DEP offers a practical solution for detecting overlooked bacteria in conventional methods and exploring their diversity and functional roles, with potential contributions to aquaculture and environmental biotechnology.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10441-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Potential of Marine Sidestreams in the Blue Economy: Lessons Learned from the EcoeFISHent Project on Fish Collagen","authors":"Lorenzo Dondero,&nbsp;Giulia De Negri Atanasio,&nbsp;Francesca Tardanico,&nbsp;Erica Lertora,&nbsp;Raffaella Boggia,&nbsp;Vittorio Capra,&nbsp;Agnese Cometto,&nbsp;Mattia Costamagna,&nbsp; Fi.L.S.E,&nbsp;Mirvana Feletti,&nbsp;Fulvio Garibaldi,&nbsp;Federica Grasso,&nbsp;Marte Jenssen,&nbsp;Luca Lanteri,&nbsp;Kjersti Lian,&nbsp;Marco Monti,&nbsp;Massimo Perucca,&nbsp;Cecilia Pinto,&nbsp;Ilaria Poncini,&nbsp;Federica Robino,&nbsp;Junio Valerio Rombi,&nbsp;Syed Saad Ahsan,&nbsp;Nikta Shirmohammadi,&nbsp;Micaela Tiso,&nbsp;Federica Turrini,&nbsp;Marta Zaccone,&nbsp;Matteo Zanotti-Russo,&nbsp;Ilaria Demori,&nbsp;Pier Francesco Ferrari,&nbsp;Elena Grasselli","doi":"10.1007/s10126-025-10438-9","DOIUrl":"10.1007/s10126-025-10438-9","url":null,"abstract":"<div><p>This review provides a general overview of collagen structure, biosynthesis, and biological properties, with a particular focus on marine collagen sources, especially fisheries discards and by-catches. Additionally, well-documented applications of collagen are presented, with special emphasis not only on its final use but also on the processes enabling sustainable and safe recovery from materials that would otherwise go to waste. Particular attention is given to the extraction process, highlighting key aspects essential for the industrialization of fish sidestreams, such as hygiene standards, adherence to good manufacturing practices, and ensuring minimal environmental impact. In this context, the EcoeFISHent projects have provided valuable insights, aiming to create replicable, systemic, and sustainable territorial clusters based on a multi-circular economy and industrial symbiosis. The main goal of this project is to increase the monetary income of certain categories, such as fishery and aquaculture activities, through the valorization of underutilized biomass.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10438-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction: Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)","authors":"Shuo Li,&nbsp;Yuyan Liu,&nbsp;Qian Wang,&nbsp;Zhiwen Zhu,&nbsp;Weijing Li,&nbsp;Chen Li,&nbsp;Fan Fei,&nbsp;Baoliang Liu,&nbsp;Changwei Shao","doi":"10.1007/s10126-025-10442-z","DOIUrl":"10.1007/s10126-025-10442-z","url":null,"abstract":"","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides from Thermolytic Hydrolysate of Arthrospira platensis","authors":"Nur Maulida Safitri,&nbsp;Jue-Liang Hsu","doi":"10.1007/s10126-025-10437-w","DOIUrl":"10.1007/s10126-025-10437-w","url":null,"abstract":"<div><p>Angiotensin-I Converting Enzyme (ACE, EC 3.4.15.1) plays an essential role in controlling blood pressure. In this research, ACE inhibitors extracted from <i>Arthrospira platensis</i> thermolysin protease were provided using various chromatographic techniques, including reversed-phase high-performance liquid chromatography (RP-HPLC) and strong cation exchange chromatography (SCX). The amino acid sequence was determined by liquid-chromatography-tandem mass spectrometry (LC–MS/MS) and identified using two independent approaches: database-assisted identification and de novo sequencing. FY11 (FSESSAPEQHY) and IR5 (ILLYR) were established with the m/z 1281.54 and 677.37, respectively. The IC₅₀ of IR5 from triplicate experiments was lower than FY11, with the value 10.54 ± 1.38 µM. IR5 was regarded as a non-competitive ACE inhibitor, with the docking interaction energy of − 106.842 kJ/mol. Docking results revealed that the interaction between ACE and peptide existed outside the ACE active site, excluding Arg 522, one of the Cl₂ binding sites. Notably, the content of IR5 in 2 mg of crude thermolysin digest was determined to be 2.42 µg/mg using LC–MS/MS quantification. Based on all these features, <i>Arthrospira</i> peptides can be considered to be a potentially promising antihypertensive agent.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptides from Dalian Stichopus japonicus: Antioxidant Activity and Melanogenesis Inhibition In Vitro Cell Models and In Vivo Zebrafish Models Guided by Molecular Docking Screening","authors":"Xue Li,&nbsp;Fanying Meng,&nbsp;Tong Sun,&nbsp;Zezhuang Hao,&nbsp;Yihua Wang,&nbsp;Yunfei Jiang,&nbsp;Ye Wang,&nbsp;Yong Li,&nbsp;Yuling Ding","doi":"10.1007/s10126-025-10433-0","DOIUrl":"10.1007/s10126-025-10433-0","url":null,"abstract":"<div><p>This article aims to reveal the optimal peptide segment with antioxidant activity from Dalian <i>Stichopus japonicus</i>, investigate its anti-melanogenesis effect, and elucidate its mechanisms of action both in vitro and in vivo. The best antioxidant alcalase hydrolysates, identified by the previous screening of proteases, was isolated by ultrafiltration; it was found that the components with a molecular weight of ≤ 3 kDa exhibited the best activity. The chemical components were characterized using LC–MS/MS. Through molecular docking, GPIGF was identified as the peptide segment with the best antioxidant and melanogenesis-inhibitory activity. A search in the NCBI database revealed that GPIGF is a newly obtained natural oligopeptide. Further experiments with synthesized GPIGF in vitro showed that it effectively reduces cell apoptosis and damage, and inhibits the expression of melanin-related genes, including tyrosinase (TYR), and associated proteins TRP-1, TRP-2, and MITF. In vivo experiments with the zebrafish model demonstrated that GPIGF significantly inhibits AAPH-induced apoptosis in zebrafish larvae, reduces the production of ROS, and suppresses melanin generation on the skin surface without exhibiting embryotoxicity. This study provides a research foundation for the development of antioxidants from Dalian <i>Stichopus japonicus</i>, which could serve as natural whitening and anti-aging agents, supporting its integrated utilization and development.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}