Marine Biotechnology最新文献

{"title":"Study on the Mechanism and Potential of Corbicula fluminea (Asian Clam) in Removing Copper and Cadmium from Aquaculture Ponds.","authors":"Jiahua Zhang, Jie Wang, Zhaojun Gu, Xingguo Liu","doi":"10.1007/s10126-024-10389-7","DOIUrl":"https://doi.org/10.1007/s10126-024-10389-7","url":null,"abstract":"<p><p>The issue of heavy metal pollution in aquaculture ponds is becoming increasingly severe, posing a significant threat to the healthy development of the aquaculture industry. Heavy metals such as cadmium and copper accumulate in ponds, not only exerting toxic effects on aquatic organisms and affecting their growth and reproduction but also endangering human health through the food chain. Bioremediation, as a green and environmentally friendly technology, utilizes specific organisms to absorb, transform, and immobilize heavy metals. We examined metal accumulation, traditional metal-related biomarkers, alongside transcriptomic and tissue histological analyses, in the hepatopancreas of Corbicula fluminea following a 14-day exposure to copper (20 µg/L), cadmium (20 µg/L), or combined copper-cadmium treatments (20 µg/L Cu and 20 µg/L Cd). Metal exposure led to notable metal accumulation in the clam's hepatopancreas. Analysis of traditional biomarkers revealed signs of cellular injury and oxidative stress in clams post-metal exposure. Transcriptomic analysis across the three treatment groups revealed disruptions in immune response, response to metal ion, and energy metabolism, characterized by differential expression levels of key genes such as ABCA3, MYD88, TOLLIP, TBK1, C2, C4, c-Myc, SYK, and SAMHD1. These findings deepen our understanding of the adverse effects of metal exposure on freshwater organisms and evaluate the potential of Corbicula fluminea for removing heavy metals from aquaculture ponds.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":"2"},"PeriodicalIF":2.6,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic Temperature Influences the Mucosal Responses of Atlantic Salmon Alevins to a Bacterial Challenge.","authors":"Muhammad Salman Malik, Alexander Rebl, Erik Burgerhout, Carlo C Lazado","doi":"10.1007/s10126-024-10386-w","DOIUrl":"https://doi.org/10.1007/s10126-024-10386-w","url":null,"abstract":"<p><p>The present work investigated the effects of embryonic temperature on the responses of Atlantic salmon (Salmo salar) alevins to a bacterial challenge using Yersinia ruckeri as a model pathogen. Embryos were reared at 4 °C, 6 °C, and 8 °C from fertilization to the eyed-egg stage. Alevins, before the start of feeding, were challenged with the pathogen, and mortality and early immune responses in mucosal organs were assessed. Fish from the 4 °C and 6 °C groups exhibited higher survival probabilities than those from the 8 °C group 72 h post-infection. Mild histopathological changes were observed in the gills and skin across all temperature groups, with bacterial antigen detected in the secondary lamellae of gills and in the skin epithelial and basal layers. Gene expression profiling revealed slightly distinct immune gene expression patterns in low-temperature groups (4 °C and 6 °C) compared to the 8 °C group. Gelsolin (gsn) expression increased in the skin across all temperature groups at 72 h post-infection. Claudin (cldn4) and collagen (col1a) were only upregulated in the skin of the 4 °C group, while heat shock protein 70 (hspa1a) was downregulated in the gills of infected fish at 72 h compared to controls. Toll-like receptor 13 (tlr13) expression increased in infected fish at 24 h compared to controls. In the 6 °C and 8 °C groups, gsn expression also increased at 72 h post-infection. Cldn4 expression increased only in the gills of 8 °C infected fish. This study revealed that low embryonic temperature could influence survival and mucosal immune defences following a bacterial challenge in Atlantic salmon alevins.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":"1"},"PeriodicalIF":2.6,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Variation of Amphiprion Percula (Lacepède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951","authors":"Jose Priya T. A.,&nbsp;Charutha Karunakaran,&nbsp;Aishwarya Nath,&nbsp;Sudha Kappalli","doi":"10.1007/s10126-023-10246-z","DOIUrl":"10.1007/s10126-023-10246-z","url":null,"abstract":"<div><p><i>Cryptocaryon irritans</i> (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-<i>C</i>. <i>irritans</i>’ molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for <i>C. irrtians</i>-infected gills of the clownfish <i>Amphiprion percula</i> (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEGs showed a vast genetic variation related to the following aspects: ECM-receptor interaction, P13K-Akt signalling, cytokine-cytokine receptor interaction, and endocytosis. During the early phase of infection, key genes involved in ATP production, energy homeostasis, and stress control were abruptly increased. In the late phase, however, acute response molecules of the peripheral nervous system (synaptic transmission and local immunity), metabolic system triggering glycogen synthesis, energy maintenance, and osmoregulation were found to be critical. The highest number of upregulated genes (URGs) recovered during the early phase was included under the ‘biological process’ category, which primarily functions as response to stimuli, signalling, and biological regulation. In the late phase, most of the URGs were related to gene regulation and immune system processes under ‘molecular function’ category. The immune-related URGs of early infection include major histocompatibility complex (MHC) class-II molecules apparently triggering CD4+ T-cell–activated Th responses, and that of late infection include MHC class-1 molecules for the possible culmination of CD8+ T-cell triggered cytotoxicity. The high level of genic single nucleotide polymorphisms (SNPs) identified during the late phase of infection is likely to influence their susceptibility to secondary infection. In summary, the identified DEGs and their related metabolic and immune-related pathways and the SNPs may provide new insights into coordinating the immunological events and improving resistance in Pomacentridae fishes against <i>C. irritans</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 6","pages":"858 - 890"},"PeriodicalIF":2.6,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10258700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrodotoxins in Larval Development of Ribbon Worm Cephalothrix cf. simula (Palaeonemertea, Nemertea)","authors":"Grigorii V. Malykin,&nbsp;Peter V. Velansky,&nbsp;Daria I. Melnikova,&nbsp;Timur Yu. Magarlamov","doi":"10.1007/s10126-023-10249-w","DOIUrl":"10.1007/s10126-023-10249-w","url":null,"abstract":"<div><p>The toxic ribbon worm, <i>Cephalothrix</i> cf. <i>simula</i> (Palaeonemertea, Nemertea), possesses extremely high concentrations of tetrodotoxin (TTX). Although TTX has been found in the eggs of this species, the fate of the toxin in the ontogeny of the animal has not been explored. Here, using high performance liquid chromatography with tandem mass spectrometry and immunohistochemistry with anti-TTX antibodies, we examined levels, profile, and localization of TTX and its analogues (TTXs) in larvae of <i>C</i>. cf. <i>simula</i> throughout 41 days post-fertilization. A detailed investigation of cells in sites of TTX-accumulation was performed with light and electron microscopy. Newly hatched larvae possessed weak TTX-like immunoreactivity in all cells. With subsequent development, intensity of TTX-labeling in the ectodermal structures, mesodermal cells and apical cylinder of the apical gland increased. In the ectodermal structures, an intense TTX-labeling was observed in the multiciliated, type II granular, type I mucoid, and basal cells of the epidermis, and in the type III granular cells of the mouth gland. In the mesoderm, TTX was localized in the muscle and unigranular parenchyma-like cells. Eggs and larvae of <i>C</i>. cf. <i>simula</i> contained five TTXs, with two major toxins – TTX and 5,6,11-trideoxyTTX. Level and relative proportion of TTXs did not differ significantly among developmental stages, confirming that larvae obtained toxins from maternal eggs and were able to retain it. The results of this study provide insights into the formation of TTX-bearing apparatus of <i>C</i>. cf. <i>simula</i> through the larval development.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 6","pages":"918 - 934"},"PeriodicalIF":2.6,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Utilization of Reference-Guided Assembly and In Silico Libraries Improves the Draft Genome of Clarias batrachus and Culter alburnus","authors":"Kai Liu,&nbsp;Nan Xie,&nbsp;Yuxi Wang,&nbsp;Xinyi Liu","doi":"10.1007/s10126-023-10248-x","DOIUrl":"10.1007/s10126-023-10248-x","url":null,"abstract":"<div><p>Long-read sequencing technologies can generate highly contiguous genome assemblies compared to short-read methods. However, their higher cost often poses a significant barrier. To address this, we explore the utilization of mapping-based genome assembly and reference-guided assembly as cost-effective alternative approaches. We assess the efficacy of these approaches in improving the contiguity of <i>Clarias batrachus</i> and <i>Culter alburnus</i> draft genomes. Our findings demonstrate that employing an iterative mapping strategy leads to a reduction in assembly errors. Specifically, after three iterations, the Mismatches per 100 kbp value for the <i>C. batrachus</i> genome decreased from 2447.20 to 2432.67, reaching a minimum of 2422.67 after two iterations. Additionally, the N50 value for the <i>C. batrachus</i> genome increased from 362,143 to 1,315,126 bp, with a maximum of 1,315,403 bp after two iterations. Furthermore, we achieved Mismatches per 100 kbp values of 3.70 for the reference-guided assembly of <i>C. batrachus</i> and 0.34 for <i>C. alburnus</i>. Correspondingly, the N50 value for the <i>C. batrachus</i> and <i>C. alburnus</i> genomes increased from 362,143 bp and 3,686,385 bp to 2,026,888 bp and 43,735,735 bp, respectively. Finally, we successfully utilized the improved <i>C. batrachus</i> and <i>C. alburnus</i> genomes to compare genome studies using the combined approach of Ragout and Ragtag. Through a comprehensive comparative analysis of mapping-based and reference-guided genome assembly methods, we shed light on the specific contributions of reference-guided assembly in reducing assembly errors and improving assembly continuity and integrity. These advancements establish reference-guided assembly and the utilization of in silico libraries as a promising and suitable approach for comparative genomics studies.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 6","pages":"907 - 917"},"PeriodicalIF":2.6,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra","authors":"Zixuan E,&nbsp;Peng Luo,&nbsp;Chunhua Ren,&nbsp;Chuhang Cheng,&nbsp;Wenjie Pan,&nbsp;Xiao Jiang,&nbsp;Fajun Jiang,&nbsp;Bo Ma,&nbsp;Suzhong Yu,&nbsp;Xin Zhang,&nbsp;Ting Chen,&nbsp;Chaoqun Hu","doi":"10.1007/s10126-023-10239-y","DOIUrl":"10.1007/s10126-023-10239-y","url":null,"abstract":"<div><p>The tropical sea cucumber <i>Holothuria scabra</i> is naturally found in the Indo-West Pacific. However, due to their commercial value, natural <i>H. scabra</i> populations have declined significantly in recent years, resulting in its status as an endangered species. Surveys of <i>H. scabra</i> resource pose a challenge due to its specific characteristics, such as sand-burrowing behavior. To overcome this problem, our study established a convenient and feasible method for assessing <i>H. scabra</i> resources using environmental DNA (eDNA) monitoring technology. First, <i>H. scabra</i>-specific TaqMan primers and probe were designed based on its <i>cox</i>1 gene, followed by the development of an eDNA monitoring method for <i>H. scabra</i> in two separate sea areas (Xuwen and Daya Bay). The method was subsequently employed to investigate the distribution of <i>H. scabra</i> and assess the effects of aquaculture stock enhancement through juvenile releasing in the Weizhou Island sea area. The <i>H. scabra</i> eDNA monitoring approach was found to be more appropriate and credible than traditional methods, and a positive impact of stocking on <i>H. scabra</i> populations was observed. In summary, this is the first report to quantify eDNA concentration in a Holothuroidea species, and it provides a convenient and accurate method for surveying <i>H. scabra</i> resources. This study introduces novel concepts for eDNA-based detection of endangered marine benthic animals and monitoring their population distribution and abundance.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 5","pages":"778 - 789"},"PeriodicalIF":3.0,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Stress Induces Apoptosis in Silver Pomfret via DUSP-JNK Pathway","authors":"Jiabao Hu,&nbsp;Man Zhang,&nbsp;Kaiheng Yan,&nbsp;Youyi Zhang,&nbsp;Yaya Li,&nbsp;Jiajie Zhu,&nbsp;Guanlin Wang,&nbsp;Xiangbing Wang,&nbsp;Yuanbo Li,&nbsp;Xiang Huang,&nbsp;Jie Tang,&nbsp;Rongyue Zheng,&nbsp;Shanliang Xu,&nbsp;Danli Wang,&nbsp;Yajun Wang,&nbsp;Xiaojun Yan","doi":"10.1007/s10126-023-10245-0","DOIUrl":"10.1007/s10126-023-10245-0","url":null,"abstract":"<div><p>We cultured silver pomfret for 20 days, decreasing water temperature from 18 to 8 ℃, and sampled muscle every 5 days. Muscle fiber degeneration and apoptosis began to increase at 13 ℃ detected by HE and TUNEL staining. Further analysis of transcriptome revealed that several apoptosis-related pathways were highly enriched by differentially expressed genes (DEGs). We analyzed 10 DEGs from these pathways by RT-qPCR during the temperature-decreasing process. <i>JNK1</i>, <i>PIDD</i>, <i>CytC</i>, <i>Casp 3</i>, and <i>GADD45</i> were up-regulated after 15 and 20 days, while <i>DUSP3</i>, <i>JNK2</i>, and <i>PARP</i> genes were down-regulated after 15 and 20 days. <i>DUSP5</i> was up-regulated from 10 to 20 days, and <i>C-JUN</i> was up-regulated after 20 days. We analyzed apoptosis in PaM cells under different temperatures (26 ℃, 23 ℃, 20 ℃, 17 ℃, and 14 ℃). The cell viability significantly declined from 14 to 20 ℃; the TUNEL and IHC results showed that the apoptosis signal increased with the temperature dropping, especially in 17 ℃ and 14 ℃; <i>DUSP5</i>, <i>JNK1</i>, <i>CytC</i>, <i>C-JUN</i>, <i>Casp 3</i>, and <i>GADD45</i> were up-regulated at 17 ℃ and 14 ℃, and <i>PIDD</i> was up-regulated at 20 ℃, 17 ℃, and 14 ℃. <i>DUSP3</i> was up-regulated at 20 ℃ but down-regulated at 17 ℃ and 14 ℃, and <i>PARP</i> was down-regulated at 17 ℃ and 14 ℃. <i>JNK2</i> was up-regulated at 20 ℃ but down-regulated at 17 ℃ and 14 ℃. Our results suggest that DUSP could help inhibit apoptosis in the initial stage of cold stress, but low temperature could down-regulate it and up-regulate JNK-C-JUN, inducing apoptosis in a later stage. These data provide a basis for the study of the response mechanism of fish to cold.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 6","pages":"846 - 857"},"PeriodicalIF":2.6,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10142959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Microplastics and Microcystin-LR Effect for Asian Clams (Corbicula fluminea) by a Metabolomics Approach","authors":"Jiahua Zhang,&nbsp;Jie Wang,&nbsp;Xiaodong Wang,&nbsp;Shikun Liu,&nbsp;Liang Zhou,&nbsp;Xingguo Liu","doi":"10.1007/s10126-023-10238-z","DOIUrl":"10.1007/s10126-023-10238-z","url":null,"abstract":"<div><p>Microplastics (MP) and microcystins (MCs) are two co-occurring pollutants in freshwater ecosystems that pose significant risks to aquatic organisms and human health. This study investigates the interactions between MP and MCs and their effects on the metabolic responses of freshwater aquaculture. Asian clams have been used as an indicator of microplastic pollution in freshwater ecosystems. The present study investigates metabolic responses of Asian clams during microplastic and microcystin-LR stress to identify health impacts and elucidate mechanistic effects of external stressors on Asian clams. A liquid chromatography/mass spectrometry (LC–MS)-based metabolomics approach was used to identify metabolic perturbations and histological section technique was used to assess changes of tissues from different Asian clam treatment groups. The results showed significantly pathological changes in the gills and hepatopancreas in experimental clam compared to control (healthy) clam. Metabolomics revealed alterations of many metabolites in the hepatopancreas of six Asian clam comparison groups, reflecting perturbations in several molecular pathways, including energy metabolism, amino acid metabolism, protein degradation/tissue damage, and oxidative stress. Overall, this study emphasizes the importance of understanding the interactions between MP and MCs and the need for proactive measures to safeguard freshwater ecosystems and human health.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 5","pages":"763 - 777"},"PeriodicalIF":3.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10122681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Characteristics of Okamejei kenojei and the Implications to Its Evolutionary Biology Study","authors":"Na Song,&nbsp;Siyu Ma,&nbsp;Xiang Zhao,&nbsp;Jiabao Zhao,&nbsp;Linlin Zhao","doi":"10.1007/s10126-023-10242-3","DOIUrl":"10.1007/s10126-023-10242-3","url":null,"abstract":"<div><p><i>Okamejei kenojei</i> is an important economic species widely distributed in shallow coastal waters of the western North Pacific. In this study, the whole-genome survey analysis of <i>O. kenojei</i> was conducted to reveal its genomic characteristics. The genome size was estimated to be 2027.44 Mb, the repeat sequence content was 44.90%, and the heterozygous ratio was 1.04%. The mitochondrial genome excavated from the sequencing data was 16,974 bp, and it can form the closed circular molecule. The phylogenetic tree based on 13 protein-coding gene sequences supported the validity of <i>Okamejei</i> and assisted the conclusion that <i>Raja porosa</i> was the junior synonym of <i>O. kenojei</i>. Plenty of potential microsatellite loci were identified, and the distribution frequency was estimated to be approximately 236.3 SSRs per Mb. Among all motif types of microsatellites, the dinucleotide repeats were dominant (82.59%), followed by the trinucleotide repeats (8.05%), tetranucleotide repeats (5.80%), pentanucleotide repeats (2.83%), and hexanucleotide repeats (0.72%). The results of the present study could not only provide useful information for understanding the genome structure and functional characteristics of <i>O. kenojei</i>, but also lay the foundation for the subsequent mapping of the whole genome.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 5","pages":"815 - 823"},"PeriodicalIF":3.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops","authors":"Tieying Yu,&nbsp;Junhao Ning,&nbsp;Fukai Wang,&nbsp;Guilong Liu,&nbsp;Quanchao Wang,&nbsp;Xin Xu,&nbsp;Chunde Wang,&nbsp;Xia Lu","doi":"10.1007/s10126-023-10247-y","DOIUrl":"10.1007/s10126-023-10247-y","url":null,"abstract":"<div><p>The interspecific hybrid scallops generated from the hermaphroditic bay scallops (<i>Argopecten irradians</i>) and Peruvian scallops (<i>Argopecten purpuratus</i>) showed significant heterosis in growth. However, its sterility limits large-scale hybridization and hinders the development of the scallop breeding industry. Hybrid sterility is regulated by plenty of genes and involves a range of biochemical and physiological transformations. In this study, whole-genome re-sequencing and transcriptomic analysis were performed in sterile and fertile hybrid scallops. The potential genetic variations and abnormally expressed genes were detected to explore the mechanism underlying hybrid sterility in hermaphroditic <i>Argopecten</i> scallops. Compared with fertile hybrids, 24 differentially expressed genes (DEGs) with 246 variations were identified to be related to fertility regulation, which were mainly enriched in germarium-derived egg chamber formation, spermatogenesis, spermatid development, mismatch repair, mitotic and meiotic cell cycles, Wnt signaling pathway, MAPK signaling pathway, calcium modulating pathway, and notch signaling pathway. Specifically, variation and abnormal expression of these genes might inhibit the progress of mitosis and meiosis, promote cell apoptosis, and impede the genesis and maturation of gametes in sterile hybrid scallops. Eleven DEGs (<i>XIAP</i>, <i>KAZN</i>, <i>CDC42</i>, <i>MEIS1</i>, <i>SETD1B</i>, <i>NOTCH2</i>, <i>TRPV5</i>, M- <i>EXO1</i>, <i>GGT1</i>, <i>SBDS</i>, and <i>TBCEL</i>) were confirmed by qRT-PCR validation. Our findings may enrich the determination mechanism of hybrid sterility and provide new insights into the use of interspecific hybrids for extensive breeding.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"25 6","pages":"891 - 906"},"PeriodicalIF":2.6,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10076897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}