Marine Biotechnology最新文献

Mariculture Probiotics: A Scoping Review of Research Trends, Knowledge Gaps and Opportunities. 海水养殖益生菌：研究趋势，知识差距和机遇的范围审查。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-04 DOI: 10.1007/s10126-026-10701-7

Lucien Alperstein, Jadranka Nappi, Suhelen Egan

{"title":"Mariculture Probiotics: A Scoping Review of Research Trends, Knowledge Gaps and Opportunities.","authors":"Lucien Alperstein, Jadranka Nappi, Suhelen Egan","doi":"10.1007/s10126-026-10701-7","DOIUrl":"https://doi.org/10.1007/s10126-026-10701-7","url":null,"abstract":"The use of probiotics - live microorganisms - to enhance growth and prevent disease in mariculture species is an emerging alternative to conventional methods including antibiotics, vaccines and chemical intervention. While others have reviewed specific host organisms and probiotic genera, this review synthesises the scientific literature on the use of probiotics across a range of marine organisms to identify knowledge gaps and research opportunities. We identified widespread methodological heterogeneity, making direct inter-study comparisons difficult. Despite this limitation, 86% of in vivo studies documented positive outcomes following probiotic administration. Crustacean and finfish research dominates the field, encompassing 77% of the current literature, while molluscs, macroalgae and other invertebrates remain relatively understudied despite their significant ecosystem services and contribution to aquaculture. Probiotics provide multiple reported benefits to marine hosts, including increased growth and survival, and protection against pathogens. Bacillus and Lactobacillus from terrestrial environments are the dominant probiotics used across all studies, while marine-derived probiotic genera including Pseudoalteromonas, Vibrio, Phaeobacter, Shewanella, and Halomonas showed benefits across several hosts but remain relatively underutilised. Approximately 34% of publications evaluated the use of microorganisms isolated from the same host species in which the probiotics were tested. This review highlights the opportunity for further research into underutilised autochthonous probiotic genera and development of standardised research guidelines. We propose that future research should prioritise understanding probiotic mechanisms of action, and their use in rapidly expanding and understudied systems including macroalgae and molluscs, to ensure continued productivity with minimal impact on marine ecosystems.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Amplicon Panel for High-Throughput and Low-Cost Genotyping of Yesso Scallop Mizuhopecten yessoensis. 一种高通量低成本的扇贝基因分型扩增子面板。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-03 DOI: 10.1007/s10126-026-10696-1

Ben J G Sutherland, Chen Yin Walker, Denman Moody, Chelsea Bennett, Marissa Wright-LaGreca, Chloe Shamash-McLaughlin, Karen Leask, Daniel Roth, Megan Lebeuf, Timothy J Green

{"title":"An Amplicon Panel for High-Throughput and Low-Cost Genotyping of Yesso Scallop Mizuhopecten yessoensis.","authors":"Ben J G Sutherland, Chen Yin Walker, Denman Moody, Chelsea Bennett, Marissa Wright-LaGreca, Chloe Shamash-McLaughlin, Karen Leask, Daniel Roth, Megan Lebeuf, Timothy J Green","doi":"10.1007/s10126-026-10696-1","DOIUrl":"https://doi.org/10.1007/s10126-026-10696-1","url":null,"abstract":"The Yesso scallop Mizuhopecten yessoensis was imported from Japan to western Canada in the late 1980s to establish an economically viable scallop aquaculture industry. Since this time, the industry in Canada has operated with existing genetic diversity within the broodstock, which is considerably limited relative to wild populations. The sector has not been able to realise its full potential in part due to idiopathic hatchery failures and farm stock collapses due to disease outbreaks associated with the intracellular bacterial pathogen Francisella halioticida. To support Yesso scallop production and breeding, here we generate a low-density, genotyping-by-sequencing amplicon panel using single nucleotide polymorphism (SNP) markers that are evenly spaced across the M. yessoensis genome and that show high heterozygosity in Canada and Japan. The panel can also exploit the high genetic polymorphism of the M. yessoensis genome, with de novo SNP calling identifying over 2,500 high quality SNPs within the 579 sequenced amplicons. We demonstrate the utility and versatility of this new genotyping tool for breeding applications including parentage assignment, low density family-based genome-wide association study, trait heritability evaluation to determine potential for genomic selection, and species differentiation (against the weathervane scallop Patinopecten caurinus). We did not find any genomic regions significantly associated with F. halioticida resistance but did identify potential for genomic selection. We could separate the two species based on genotypes, and did not see evidence of a past M. yessoensis x P. caurinus hybridization event within the M. yessoensis breeding population at Vancouver Island University. This low-cost genotyping panel is expected to accelerate selective breeding improvements for M. yessoensis in Canada and elsewhere.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Quantitative Proteomic Profiling of Pinctada fucata Shell Nacre Defines a Solubility-Based Type Classification of Shell Matrix Proteins. 修正：fucata Pinctada Shell珍珠的定量蛋白质组学分析定义了基于溶解度的壳基质蛋白类型分类。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-03 DOI: 10.1007/s10126-026-10704-4

Keisuke Oshima, Lumi Negishi, Takashi Atsumi, Hitoshi Kurumizaka, Michio Suzuki

引用次数: 0

Optimized Extraction and Bioactivity Profiling of Phenolics from Bostrychia scorpioides (Hudson) Mont: A Sustainable Approach for Pharmaceuticals Applications. 天蝎花酚类物质的优化提取及生物活性分析：一种可持续的药物应用方法。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-02 DOI: 10.1007/s10126-026-10700-8

Kaoutar Bougadra, Amine Elbouzidi, João Cotas, Khadija El Azhary, Sara Bourdoukh, Zahira Belattmania, Abdallah Badou, Brahim Sabour, Fouzia Hmimid

{"title":"Optimized Extraction and Bioactivity Profiling of Phenolics from Bostrychia scorpioides (Hudson) Mont: A Sustainable Approach for Pharmaceuticals Applications.","authors":"Kaoutar Bougadra, Amine Elbouzidi, João Cotas, Khadija El Azhary, Sara Bourdoukh, Zahira Belattmania, Abdallah Badou, Brahim Sabour, Fouzia Hmimid","doi":"10.1007/s10126-026-10700-8","DOIUrl":"https://doi.org/10.1007/s10126-026-10700-8","url":null,"abstract":"This study presents a comprehensive approach to valorizing the red macroalga Bostrychia scorpioides (BS), collected from Oualidia lagoon, as a reservoir of compounds with biological activity. An extraction protocol combining ultrasound-assisted pretreatment with conventional maceration in 80% (v/v) hydroethanolic solvent was developed, and the recovery of phenolic compounds was maximized using response surface methodology (RSM) based on a Box-Behnken design (BBD). The model-predicted and experimentally validated optimal extraction conditions were 37.5 min of ultrasonication, an extraction temperature of 45 °C and 3.5 h of maceration, yielding a TPC of 341.45 µg GAE/g dw; the optimal extract was also rich in flavonoids and condensed tannins. In terms of bioactivity, the extract exhibited substantial antioxidant capacity, achieving 98.97% hydrogen peroxide scavenging, which strongly correlated with the phenolic concentration. Antibacterial evaluation revealed pronounced inhibition of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli, with inhibition zones ranging from 9 to 19 mm and MIC values from 3.125 to 12.5 mg/mL. Crucially, cytotoxicity assessment on PBMCs demonstrated a favorable safety profile, maintaining over 85% cell viability at concentrations up to 200 µg/mL. High-Performance Liquid Chromatography (HPLC) and molecular docking simulations identified chlorogenic acid as a major bioactive constituent, displaying a high in silico binding affinity (- 8.0 kcal/mol) for aromatase, a validated target in oncological therapy. Taken together, these findings suggest that BS represents a sustainable and safe natural reservoir of antioxidant and antimicrobial compounds of interest for natural drug development.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Validation of the Phase I-II-III Detoxification Pathway in Azumapecten farreri using Optimized RNA Interference Technology. 利用优化的RNA干扰技术对azumappecten farfareri I-II-III期解毒途径的功能验证。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-02 DOI: 10.1007/s10126-026-10698-z

Limin Lu, Guanchao Zheng, Yichen Lin, Chenfan Dong, Jixing Peng, Haiyan Wu, Zhijun Tan

{"title":"Functional Validation of the Phase I-II-III Detoxification Pathway in Azumapecten farreri using Optimized RNA Interference Technology.","authors":"Limin Lu, Guanchao Zheng, Yichen Lin, Chenfan Dong, Jixing Peng, Haiyan Wu, Zhijun Tan","doi":"10.1007/s10126-026-10698-z","DOIUrl":"https://doi.org/10.1007/s10126-026-10698-z","url":null,"abstract":"Paralytic shellfish toxins (PSTs) are widely distributed neurotoxins that accumulate and transform in bivalves, but the metabolic mechanisms after exposure of Azumapecten farreri (A. farreri) to PSTs remain unknown. To verify the phase I (cytochrome P450, CYP450)-II (glutathione S-transferase, GST)-III (ABC transport) metabolic genes for PSTs, we used RNA interference (RNAi) to silence target genes (CYP46A1, GSTM1, and ABCF2), measured PSTs content, and performed single nucleotide polymorphism (SNP) screening. Injection of small interfering RNA (siRNA) fragments into the scallop adductor muscle silenced target genes in multiple tissues. The three most effective fragments were selected. Secondary injection of screening fragments prolonged the silencing duration, with a silencing efficiency of 12.0%-100%. Silencing increased PSTs accumulation. Notably, with CYP46A1 silencing, the PSTs accumulation (1016 µg STXeq/kg) reached 3.85 times that of the control group. This silencing also delayed the conversion of highly toxic components (GTX1-4) to components with low toxicity (C1-2), thereby prolonging the PSTs metabolic process. SNP analyses indicated that PSTs exposure increased homozygous mutations in CYP49A1 (511908 site), this site is potentially involved in PSTs metabolism. In summary, this study optimized an RNAi method and confirmed the existence of a three-phase metabolic mechanism in scallops. CYP450 was found to be the main mode of PSTs metabolism. This study provides data regarding PSTs metabolic genes and loci in bivalves, as well as valuable molecular markers for anti-toxin breeding in shellfish.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus. 高效显微注射及CRISPR/Cas9介导的独角兔基因组编辑

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-02 DOI: 10.1007/s10126-026-10702-6

Kai Wang, Jiao Chen, Shumiao Bai, Qing Wang, Wei Zhang, Zhenkui Qin, Zhengrui Zhang, Zhifeng Zhang, Yubin Ma

{"title":"Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus.","authors":"Kai Wang, Jiao Chen, Shumiao Bai, Qing Wang, Wei Zhang, Zhenkui Qin, Zhengrui Zhang, Zhifeng Zhang, Yubin Ma","doi":"10.1007/s10126-026-10702-6","DOIUrl":"https://doi.org/10.1007/s10126-026-10702-6","url":null,"abstract":"Urechis unicinctus is an economically important aquaculture species and an emerging model for developmental and evolutionary research; however, progress in functional genetic studies in this species has remained limited by the absence of effective genome editing approaches. Here, we establish a CRISPR/Cas9-mediated genome editing platform in U. unicinctus based on an optimized microinjection procedure. Targeting the cilia-associated gene Caveolin-1, Cas9/sgRNA ribonucleoprotein complexes were delivered into oocytes, generating mosaic G0 larvae harboring insertion-deletion mutations, as confirmed by ICE analysis and cloning-based Sanger sequencing with mutation detection rates of 68.51% and 64.86%, respectively. Ciliary defects were frequently observed in edited larvae, including shortening of the circumoral ciliary ring and reduction of apical ciliary tufts, accompanied by impaired swimming performance. Whole-mount in situ hybridization further revealed altered spatial expression patterns of Caveolin-1 in edited embryos, with 78% of individuals showing detectable changes in expression patterns. To independently assess the robustness of the platform, the conserved cytoskeletal gene α-tubulin was additionally targeted, resulting in successful mutagenesis and associated ciliary abnormalities. Together, these findings establish the first CRISPR/Cas9 genome editing workflow for U. unicinctus, providing a technical framework for functional genomics in echiurans and facilitating future studies of developmental mechanisms and molecular breeding in this species.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Accumulation and Excretion of Tetrodotoxin and 5,6,11-Trideoxytetrodotoxin in the Mussel Mytilus galloprovincialis Following Ingestion of Toxic Flatworm Larvae. 食用有毒扁虫幼虫后，加洛省贻贝体内河豚毒素和5,6,11-三氧河豚毒素积累和排泄的差异。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-09-01 DOI: 10.1007/s10126-026-10690-7

Megumi Morimura, Wakana Yuu, Kyoko Shirai, Rei Suo, Shiro Itoi

{"title":"Differential Accumulation and Excretion of Tetrodotoxin and 5,6,11-Trideoxytetrodotoxin in the Mussel Mytilus galloprovincialis Following Ingestion of Toxic Flatworm Larvae.","authors":"Megumi Morimura, Wakana Yuu, Kyoko Shirai, Rei Suo, Shiro Itoi","doi":"10.1007/s10126-026-10690-7","DOIUrl":"https://doi.org/10.1007/s10126-026-10690-7","url":null,"abstract":"Tetrodotoxin (TTX), commonly known as pufferfish toxin, is widely distributed among marine organisms. In the past decade, TTX has been detected in edible bivalves along coasts worldwide, raising concerns about unexpected food poisoning. Recent research suggests that planktonic larvae of the TTX-bearing flatworm Planocera multitentaculata are involved in the toxification of bivalves. However, some uncertainties remain: although these flatworms contain large amounts of 5,6,11-trideoxytetrodotoxin (TDT), TDT is generally not detected in bivalves, even when TTX is present. The aim of this study was to characterize the accumulation and excretion of TTX and TDT in the mussel Mytilus galloprovincialis after ingestion of toxic P. multitentaculata larvae and to determine whether differences in their excretion could explain the rarity of TDT in naturally contaminated bivalves. LC-MS/MS analysis revealed that the toxified mussels accumulated both TTX and TDT in the midgut gland. However, the concentrations of both compounds decreased over time, with TDT showing a faster decline than TTX. These findings provide the first evidence for the accumulation and excretion of TDT in bivalves and suggest that its faster elimination contributes to its rare detection in naturally contaminated specimens. The results also indicate that detection of TDT in wild bivalves may reflect relatively recent ingestion of TTX-bearing organisms, providing new insights into TTX analog dynamics and contributing to seafood safety monitoring.","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Activity of Bioactive Peptides Derived from Ulva prolifera in HaCaT Cells and Zebrafish 增生Ulva生物活性肽在HaCaT细胞和斑马鱼中的抗氧化活性

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-08-29 DOI: 10.1007/s10126-026-10691-6

Li-Ru Hong, Shuo-Qi Wang, Ting-Ting Wu, Zhi-Ru Chen, Mo Chen, Chun-Er Cai, Pei-Min He, Ya Zhao, Rui Jia

{"title":"Antioxidant Activity of Bioactive Peptides Derived from Ulva prolifera in HaCaT Cells and Zebrafish","authors":"Li-Ru Hong, Shuo-Qi Wang, Ting-Ting Wu, Zhi-Ru Chen, Mo Chen, Chun-Er Cai, Pei-Min He, Ya Zhao, Rui Jia","doi":"10.1007/s10126-026-10691-6","DOIUrl":"10.1007/s10126-026-10691-6","url":null,"abstract":"<div>Ulva prolifera (U. prolifera), a large green alga widely distributed along coastlines worldwide, can trigger marine ecological issues such as “green tides” when over-proliferating. Nevertheless, it is abundant in various nutrients and bioactive compounds, making the utilization of this alga an urgent task. In this study, an antioxidant peptide fraction (UCP‑1) was isolated and purified from U. prolifera. LC‑MS/MS analysis indicated that the peptide composition and molecular weight distribution of UCP‑1 possess typical characteristics of antioxidant peptides. In vitro experiments demonstrated that UCP‑1 at a concentration of 1 mg mL⁻¹ exhibited a DPPH radical scavenging capacity of 38.86 ± 2.7%, indicating antioxidant activity. In human immortalized keratinocytes (HaCaT) and zebrafish models, UCP-1 pretreatment reduced hydrogen peroxide (H₂O₂)-induced reactive oxygen species (ROS) and malondialdehyde (MDA) levels while increasing superoxide dismutase (SOD) activity. In zebrafish, UCP-1 also enhanced catalase (CAT) and glutathione peroxidase (GSH-Px) activities. In addition, incorporation of UCP‑1 into lotions and creams in skin trials improved skin hydration and elasticity and reduced erythema. Overall, the peptide UCP‑1 derived from U. prolifera exerts antioxidant activity and may serve as a natural antioxidant ingredient in cosmetic formulations, thereby providing an alternative approach for the high-value utilization of U. prolifera resources.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Myo-Inositol Improves Growth, Antioxidant and Immune Responses in Bisphenol S-Exposed Procambarus clarkii 饲粮中肌醇对暴露于双酚s的克氏原螯虾生长、抗氧化和免疫应答的促进作用

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-08-25 DOI: 10.1007/s10126-026-10694-3

Changchang Pu, Yuanyi Liu, Junhui Wang, Bingke Wang, Aimin Wang, Chunnuan Zhang

{"title":"Dietary Myo-Inositol Improves Growth, Antioxidant and Immune Responses in Bisphenol S-Exposed Procambarus clarkii","authors":"Changchang Pu, Yuanyi Liu, Junhui Wang, Bingke Wang, Aimin Wang, Chunnuan Zhang","doi":"10.1007/s10126-026-10694-3","DOIUrl":"10.1007/s10126-026-10694-3","url":null,"abstract":"<div>Bisphenol S (BPS) is an emerging environmental contaminant that can disrupt oxidative and immune homeostasis in aquatic organisms, whereas myo-inositol (MI) is a water-soluble nutrient involved in growth and cellular signaling. This study evaluated growth, hepatopancreatic biochemical indices, and stress-related gene transcription in crayfish fed 0 or 1000 mg/kg supplemental MI and chronically exposed to 0–10 µg/L BPS for 6 weeks. Compared with the control group, the BPS group had significantly lower weight gain rate (WGR), specific growth rate (SGR), molting frequency, antioxidant enzyme activities (SOD, CAT, and POD), and immune enzyme activities (ACP, AKP, PO, and LZM), together with higher MDA content (P < 0.05). Compared with the BPS group, the MI + BPS group had significantly higher WGR, SGR, molting frequency, SOD, POD, ACP, and PO activities (P < 0.05). The MI + BPS group also showed higher nrf2 and hmc mRNA levels and lower keap1, bip, ire1, casp3, cytc, jnk, il-6, tnf-α, and nf-κb mRNA levels than the BPS group (P < 0.05). WGR, SGR, molting frequency, and hmc mRNA abundance in the MI + BPS group did not differ significantly from the control group (P > 0.05), the remaining endpoints showed indicator-specific responses. These findings indicate that dietary MI supplementation was associated with improved growth and selected antioxidant and immune indices, as well as altered transcription of genes related to oxidative, endoplasmic-reticulum-stress, inflammatory, and apoptosis-associated responses under BPS exposure. Pathway activation, tissue injury, and apoptosis were not directly assessed.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient Trim25 Knockdown Enhances Transfection Efficiency in Two Teleost Fish Cell Lines 瞬时敲低Trim25提高两种硬骨鱼细胞系转染效率

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-08-25 DOI: 10.1007/s10126-026-10695-2

Yajun Wang, Xiaohuan Ge, Tiange Guo, Mengda Du, Yan He

{"title":"Transient Trim25 Knockdown Enhances Transfection Efficiency in Two Teleost Fish Cell Lines","authors":"Yajun Wang, Xiaohuan Ge, Tiange Guo, Mengda Du, Yan He","doi":"10.1007/s10126-026-10695-2","DOIUrl":"10.1007/s10126-026-10695-2","url":null,"abstract":"<div>Efficient transgene expression following nucleic acid transfection remains difficult in many fish cell lines, particularly those derived from marine species. Although extracellular delivery systems have been extensively optimized, intracellular factors that limit the expression of delivered nucleic acids remain poorly understood in teleosts. In mammals, TRIM25 (tripartite motif-containing protein 25) participates in cytoplasmic exogenous RNA surveillance. Here, we examined whether its teleost homolog contributes to the low reporter expression observed after transfection. Transient siRNA-mediated knockdown of trim25 increased the percentage of eGFP-positive cells following Cap1-m¹Ψ-eGFP mRNA transfection from 19.27% to 74.63% in zebrafish embryonic fibroblast cell line (PAC2) and from 10.41% to 36.15% in Chinese tongue sole testis cell line (CST). Reporter expression following pEGFP-N1 plasmid transfection was also increased, although to a lesser extent. In this study, transfection efficiency was operationally defined as the percentage of eGFP-positive cells among total nuclei and did not directly measure nucleic acid uptake. These findings suggest that transient trim25 knockdown may alleviate intracellular restriction of exogenous reporter expression in PAC2 and CST cells and may provide an affordable approach for improving molecular manipulation in selected fish cell systems.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0