Marine Biotechnology最新文献

{"title":"Transcriptome Analysis Reveals the Key Genes and Pathways for Growth, Ion Transport, and Oxidative Stress in Post-Larval Black Tiger Shrimp (Penaeus monodon) Under Acute Low Salt Stress","authors":"Yiming Li,&nbsp;Pengcheng Gao,&nbsp;Yucong Ye,&nbsp;Yan Li,&nbsp;Zhen Sun,&nbsp;Longyi Li,&nbsp;Kai Zhou,&nbsp;Yuxing Wei,&nbsp;Zongli Yao,&nbsp;Qifang Lai","doi":"10.1007/s10126-025-10411-6","DOIUrl":"10.1007/s10126-025-10411-6","url":null,"abstract":"<div><p>As an abiotic stress factor, salinity significantly affects the physiological activities of crustaceans. In this study, transcriptome sequencing was used to evaluate the mechanism of ion transport and the physiological response of black tiger shrimp (<i>Penaeus monodon</i>) under low salt stress. Four hundred post larval (PL) stage <i>P. monodon</i> were distributed in eight experimental tanks and exposed to 3 or 5 ppt salt concentrations for 96 h. Low salinity significantly reduced the survival rate of shrimp but simultaneously activated the activity of ion transporter enzymes Na⁺/K⁺-ATPase (NKA) and Ca²⁺/Mg²⁺-ATPase), the expression of <i>NKA</i>, galectin 10, and cytochrompe c peroxidase genes, and the activity and expression of antioxidant-related genes (superoxide dismutase, catalase, heat shock protein 60). Low salt stress activated the urea cycle but significantly inhibited glutathione metabolization-related indicators (glutamate dehydrogenase, glutaminase, glutamic acid). RNA-seq analysis identified 221 differentially expressed genes (78 up-regulated and 143 down-regulated). Quantitative real-time PCR and RNA-seq results of 11 of them were consistent, illustrating the validity of the transcriptomic predictions. Gene set enrichment analysis results showed that calcium ion transmembrane transport, calmodulin binding, the stress-activated protein kinase signaling cascade, and regulation of the cytosolic calcium ion concentration process were significantly enriched. These results showed that low salt stress activated the calcium-dominated ion transport pathway and promoted molting growth of <i>P. monodon</i>. They also indicate that there is potential for larval rearing shrimp under low salt conditions.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative Splicing Events and Differently Expressed Genes During Peak Mortality in Large Yellow Croaker (Larimichthys crocea) Infected with Scuticociliate","authors":"Jian Jin,&nbsp;Yang Li,&nbsp;Xiande Liu,&nbsp;Chunmei Yan,&nbsp;Zhiqiang Cheng,&nbsp;Yannian Wu,&nbsp;Zhiyong Wang,&nbsp;Dongling Zhang","doi":"10.1007/s10126-025-10413-4","DOIUrl":"10.1007/s10126-025-10413-4","url":null,"abstract":"<div><p>Large yellow croaker (<i>Larimichthys crocea</i>) is facing various threats from bacterial, viral, and parasitic diseases, especially scuticociliate. Scuticociliate is a facultative parasite causing high mortality in various marine fishes. In this study, an artificial scuticociliate infection model was successfully established for large yellow croaker. Comparative transcriptome analysis was performed on gill tissues collected from control fish and fish at the peak of mortality following exposure to the parasite to investigate the underlying molecular mechanisms of host-parasite interactions. A total of 400, 427, and 311 differential alternative splicing (DAS) events were identified at 7 d/0 h, 8 d/0 h, and 9 d/0 h, respectively. Meanwhile, 761 differentially expressed genes (DEGs) were found, with 154 simultaneously at three time points. GO and KEGG enrichment analysis showed that DAS genes and DEGs were mainly focused on self-respire, immune, and metabolic-related pathways. The DEGs related to blood coagulation included <i>fga</i>, <i>fgb</i>, <i>fgg</i>, and lectin domain genes. Lectin domain genes were also involved in reducing parasite burden. Cytokines, Caspase-1, <i>trim13</i>, <i>trim16</i>, and <i>trim39</i> co-participated in immune response. Notably, the complement component gene <i>c3</i> was both a DEG and underwent DAS. Using STRING software, interaction regulatory networks were constructed to visualize potential hub genes, revealing 22 DEGs shared across at least two time points. These findings provide valuable insights into the immune and metabolic responses of large yellow croaker to scuticociliate infection, offering a foundational reference for identifying resistant genes and understanding fish-parasite interactions.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory Effects of the Fucoidan from Sea Cucumber Apostichopus japonicus","authors":"Tingting Hou,&nbsp;Xiaolin Liu,&nbsp;Shuai Zhang,&nbsp;Kui Tang,&nbsp;Shilin Liu,&nbsp;Jialiang Liu,&nbsp;Xiaoteng Fan,&nbsp;Zaizhao Wang","doi":"10.1007/s10126-025-10410-7","DOIUrl":"10.1007/s10126-025-10410-7","url":null,"abstract":"<div><p>Fucoidan from <i>Apostichopus japonicus</i> (Aj-FUC) has shown anti-inflammatory activity, whereas its mechanism was not explicated. This study investigated the anti-inflammatory potential and mechanism of the fucoidan from green and purple <i>A. japonicus</i> (G-FUC and P-FUC) in lipopolysaccharide (LPS)-treated RAW264.7 cells. Results showed that Aj<i>-</i>FUCs at 25–400 µg/mL had no toxicity to cells after 24 h stimulation and promoted cell phagocytic activity. ELISA results indicated that Aj<i>-</i>FUC reduced the nitric oxide (NO), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 levels and increased IL-10 level. The Aj-FUC suppressed transcription of inflammatory-related genes (<i>tnf-α, il-1β</i>, <i>il-6</i>, <i>nlrp3</i>, <i>inos</i>, <i>cox-2</i>, <i>tlr4</i>, <i>trif</i>, and <i>nf-κb</i>) in LPS-treated RAW264.7 cells, among which G-FUC had stronger anti-inflammatory effects. Moreover, Aj<i>-</i>FUC upregulated the mRNA expression of autophagic genes (<i>beclin1</i>, <i>lc3II</i>, and <i>lamp2</i>). The immunoblotting and immunofluorescence analyses of Beclin-1 and LC3II supported that Aj<i>-</i>FUC enhanced autophagy activity. After autophagy inhibited by 3-methyladenine, the mRNA expressions of <i>tnf-α</i>, <i>il-6</i>, <i>il-1β</i>, and <i>nlrp3</i> were significantly upregulated in LPS-induced cells treated with Aj-FUC, suggesting the suppressed inflammation by Aj-FUC mediated via autophagy. Summarily, the present study demonstrated that Aj<i>-</i>FUC showed anti-inflammatory effects by elevating autophagy activity in LPS-induced macrophages.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria-Produced Algicide for Field Control of Toxic Dinoflagellates Does Not Cause a Cortisol Stress Response in Two Estuarine Fish Species","authors":"Victoria E. Simons,&nbsp;Timothy E. Targett,&nbsp;Patrick M. Gaffney,&nbsp;Kathryn J. Coyne","doi":"10.1007/s10126-024-10383-z","DOIUrl":"10.1007/s10126-024-10383-z","url":null,"abstract":"<div><p>Application of algicides produced by naturally occurring bacteria is considered an environmentally friendly approach to control harmful algal blooms. However, few studies assess the effects of bacterial algicides on non-target species, either independently or with other stressors. Here, we measured sub-lethal effects of dinoflagellate-specific algicide IRI-160AA on the estuarine fish <i>Fundulus heteroclitus</i> and <i>Menidia menidia</i> in laboratory experiments. Plasma cortisol levels were measured to test whether a neuroendocrine stress response was induced in these fish following exposure to the algicide alone, and in combination with diel-cycling hypoxia and/or pH, at 25 and 30 °C. Results show that exposure to IRI-160AA does not significantly affect cortisol levels in either species, at either temperature tested, whether exposure occurs independently or with co-occurring hypoxia and/or pH cycles as potential multiple stressors. These results support the application of IRI-160AA as an environmentally friendly approach to control harmful algal blooms in estuarine environments.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10383-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation of Somatic Tissues in Oysters is Influenced by Sex and Heredity","authors":"Wen Teng,&nbsp;Chengxun Xu,&nbsp;Shikai Liu,&nbsp;Hong Yu,&nbsp;Lingfeng Kong,&nbsp;Qi Li","doi":"10.1007/s10126-024-10409-6","DOIUrl":"10.1007/s10126-024-10409-6","url":null,"abstract":"<div><p>The influence of sex and heredity on DNA methylation in the somatic tissues of mice has been well-documented, with similar hereditary effects reported in honeybees. However, the extent to which these factors affect DNA methylation in molluscan somatic tissues remains poorly understood. In this study, we investigated genomic DNA methylation patterns in the adductor muscle of two genetically distinct oyster strains using whole-genome bisulfite sequencing (WGBS). Our analysis identified significant differences in DNA methylation between sexes, with females exhibiting a global reduction compared to males. Furthermore, approximately half of the differentially methylated sites between the two parental strains were conserved in their offspring. Regions with differential methylation in parents typically exhibited intermediate methylation levels in the F1 progeny, whereas consistently methylated regions in parents maintained similar methylation levels in their progeny. These findings suggest that offspring DNA methylation is strongly influenced by parental methylation profiles, highlighting its potential role in sexual determination in oysters.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Mechanism Analysis Related to Cold Tolerance of Red Swamp Crayfish, Procambarus clarkii","authors":"Jihu Zhang,&nbsp;Cheng-long Zhang,&nbsp;Hong Ju Chen,&nbsp;Xiang Shan Ji,&nbsp;Yan Zhao","doi":"10.1007/s10126-024-10408-7","DOIUrl":"10.1007/s10126-024-10408-7","url":null,"abstract":"<div><p>In China, the red swamp crayfish (<i>Procambarus clarkii</i>), a notorious invasive species, has become an important economic freshwater species. In order to compare the genetic diversity and population structure of crayfish from northern and southern China, we collected 60 crayfish individuals from 4 crayfish populations in northern China and 2 populations in southern China for sequencing using the 2b-RAD technique. Additionally, the whole genome sequence information obtained by 2b-RAD of 90 individuals from 2 populations in northern China and 7 populations in southern China were downloaded from NCBI. After quality control, a total of 25,371 SNPs were detected from approximately 54.22 billion raw reads. Based on these SNPs, high genetic diversity was observed in the 15 crayfish populations in China. The pairwise <i>F</i>_ST values indicated that there was a large genetic differentiation of crayfish populations in northern and southern China. Despite common genetic backgrounds, due to geographical barriers, genetic divergence has been observed in northern and southern China crayfishes. The principal component analysis in combination with Admixture and Neighbor-Joining tree analysis showed that the crayfish fell into two clusters corresponding to geographical regions. The integrated analysis of whole genome and transcriptome data showed that two genes (<i>CETN4</i> and <i>CPEB2</i>) might play important roles during crayfish resistance to a cold environment. This study reveals the genetic differentiation of crayfish populations in northern and southern China and provides clues to the genetic mechanism related to cold adaptation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Chromatography Test Strip of Exonuclease III-Amplified Aptamer for Rapid Identification of Prorocentrum minimum","authors":"Fuguo Liu,&nbsp;Chunyun Zhang,&nbsp;Jiahao Li,&nbsp;Jinju Ma,&nbsp;Yu Duan,&nbsp;Yuanyuan Wang,&nbsp;Guofu Chen","doi":"10.1007/s10126-024-10407-8","DOIUrl":"10.1007/s10126-024-10407-8","url":null,"abstract":"<div><p>Recently, the scale and frequency of harmful algae blooms (HABs) have gradually increased, posing a serious threat to human health, marine ecosystems and economic development. For early warning, a method is required that can quickly detect and monitor microalgae. It is proposed to use aptamer targeted to <i>Prorocentrum minimum</i>, along with exonuclease III (Exo III), gold nanoparticles, target single-stranded DNA and hairpin structure probe to construct a new method, i.e. aptamer-lateral flow dipstick (LFD) based on Exo III-assisted signal amplification assay (ALBEA). The key conditions, including signal amplification and LFD detection, are optimized. Under the optimal conditions, the detection limit of ALBEA was 1.25 cells mL⁻¹. The cross-reactivity test showed no positive result except for <i>P. minimum</i>, indicating that the method is highly specific. The anti-interference test confirmed that the technique was not affected by the presence of other microalgae. The tested results of <i>P. minimum</i> cultured under different nutrient conditions and different growth stages demonstrated that the method is not affected by the cell state. Furthermore, the test results of simulated natural water samples further validated the practicality of the ALBEA. In conclusion, the established ALBEA offers a sensitive, specific and user-friendly tool, which can be used for the rapid detection of <i>P. minimum</i> and also provides a reference for the detection of other microalgae.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Basis and Identification of Candidate Genes for Alkalinity Tolerance Trait in Spotted Sea Bass (Lateolabrax maculatus) by Genome-Wide Association Study (GWAS)","authors":"Yonghang Zhang,&nbsp;Chong Zhang,&nbsp;Haishen Wen,&nbsp;Xin Qi,&nbsp;Qing Wang,&nbsp;Kaiqiang Zhang,&nbsp;Lingyu Wang,&nbsp;Donglei Sun,&nbsp;Yani Dong,&nbsp;Pengyu Li,&nbsp;Yun Li","doi":"10.1007/s10126-024-10405-w","DOIUrl":"10.1007/s10126-024-10405-w","url":null,"abstract":"<div><p>Given the challenges of overcrowded coastal aquaculture spaces and insufficient production, utilizing saline-alkaline water areas represents a vital strategy to alleviate these bottlenecks. Spotted sea bass (<i>Lateolabrax maculatus</i>), with its formidable osmoregulatory capabilities, is an ideal candidate to develop a saline-alkaline tolerant strain. In our study, genotypic and phenotypic data from 287 <i>L. maculatus</i> individuals exposed to carbonate alkaline conditions were collected, and a genome-wide association study (GWAS) conducted to elucidate genetic basis related to carbonate alkalinity tolerance trait. Results showed that 14 SNPs and 8 InDels were markedly related to carbonate alkalinity tolerance trait, and 404 candidate genes were pinpointed within a ± 300-kb region surrounding these variants. Notably, the most significant SNP (SNP_05_17240108), along with two adjacent SNPs (SNP_05_17240102 and SNP_05_17240340) and two InDels (InDel_05_17240228 and InDel_05_17240231), was situated in the intron region of <i>trio</i> gene that could play vital roles in cell remodeling, and cell junction and activity of aquaporins to deal with carbonate alkalinity stress. Furthermore, candidate genes were significantly involved in pathways associated with carbohydrate metabolism, cell remodeling, ion transport, and RNA degradation, which were consistent with RNA-Seq analysis results of gills and kidneys in response to alkalinity stress. Our study will contribute to elucidate the genetic basis of alkalinity tolerance and the identified SNPs and InDels could be used for marker-assisted selection (MAS) and genomic selection (GS) for alkalinity tolerance trait in the breeding programs of spotted sea bass.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sargassum glaucescens Extract/Marine-Derived Collagen Blend Sponge and Their Properties for Wound Healing","authors":"Tayyeb Ghadimi,&nbsp;Noorahmad Latifi,&nbsp;Ahmad Hivechi,&nbsp;Vahid Hosseinpour Sarmadi,&nbsp;Sina Bayat Shahbazi,&nbsp;Naser Amini,&nbsp;Peiman B. Milan,&nbsp;Abolfazl Abbaszadeh,&nbsp;Ghazaleh Larijani,&nbsp;Hamid Fathalian,&nbsp;Shokoufeh Mortazavi,&nbsp;Fatemeh Latifi,&nbsp;Fatemeh Ghadimi,&nbsp;Siamak Farokh Forghani,&nbsp;Soheila Naderi Gharahgheshlagh","doi":"10.1007/s10126-024-10402-z","DOIUrl":"10.1007/s10126-024-10402-z","url":null,"abstract":"<div><p>Burn wounds are challenging to treat due to considerable tissue damage and fluid loss. Creating wound dressings from natural and biological materials makes it possible to treat wounds and promote rapid epithelialization to speed healing and restore skin function. As a result, the ability of a collagen scaffold (Col) made from rainbow trout (<i>Oncorhynchus mykiss</i>) and putative bioactive phytochemical components from a <i>Sargassum glaucescens (S. glaucescens)</i> extract (SGE) to promote burn wound healing was assessed in this work. Synthesized collagen (40 mg/ml)/SGE (1–3 mg/ml) samples were then characterized physiochemically and physiologically. The physicochemical examination validated the bioactive component of SGE, the type of collagen (type I, α1, and α2), the successful incorporation of SGE into collagen scaffolds (Col/SGE), the thermal stability, and excellent hydrophilicity and water absorption capacity of produced scaffolds. Moreover, biological experiments approved the excellent antioxidant and antibacterial activity of SGE, structural stability improvement against degradation, and cell proliferation enhancement without cell toxicity. The results showed the Col/SGE 3 mg/ml sample also had the highest level of cell activity, according to the antibacterial and cell viability assays. Additionally, using Col/SGE in vivo on burn wounds in rat models demonstrated a quicker rate of wound healing with stronger re-epithelialization and dermal remodeling, fewer inflammatory cells, more fibroblast cells, and great collagen buildup. Therefore, since the collagen/SGE scaffold is structurally stable and can potentially promote cell proliferation without causing cell toxicity, the acquired results suggested that it may significantly impact wound healing.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142919053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Single Nucleotide Polymorphisms in Cysteamine Dioxygenase Gene with Taurine Content in Oyster Crassostrea gigas","authors":"Shiqing Sun,&nbsp;Ning Kong,&nbsp;Xiang Li,&nbsp;Chunyu Jiang,&nbsp;Mingyu Xue,&nbsp;Lingling Wang,&nbsp;Linsheng Song","doi":"10.1007/s10126-024-10404-x","DOIUrl":"10.1007/s10126-024-10404-x","url":null,"abstract":"<div><p>The Pacific oyster <i>Crassostrea gigas</i> is rich in taurine, a conditionally essential amino acid functioning in anti-oxidation, anti-inflammation, anti-aging, osmoregulation, and neuromodulation. Breeding oyster varieties with enhanced taurine content is significant to meet people’s demand for high-quality oysters. In the present study, polymorphisms in the oyster cysteamine dioxygenase (<i>Cg</i>ADO) gene that encodes the central enzyme of the cysteamine pathway for taurine synthesis were investigated, and their association with taurine content was assessed in the Changhai (CH) and Qinhuangdao (QHD) populations. A total of 47 single nucleotide polymorphism (SNP) loci were identified in the exonic region of <i>Cg</i>ADO through Sanger sequencing, with a synonymous SNP (c.415T&gt;C) showing a significant association with taurine content. Oysters with the CT genotype at c.415T&gt;C exhibited higher taurine content than those with the TT genotype (<i>p</i> &lt; 0.05). Moreover, a significant difference in the <i>Cg</i>ADO mRNA expression was observed between oysters with different genotypes, with higher expression in the CT genotype compared to the TT genotype (<i>p</i> &lt; 0.001). These findings indicate the potential influence of <i>Cg</i>ADO polymorphisms on taurine content in <i>C. gigas</i> and provide candidate functional markers for the selective breeding of oyster varieties with improved taurine levels.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142919052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}