Marine Biotechnology最新文献

Mechanism of Crassostrea gigas T-box Transcription Factor 2: Regulation by a Transcript Isoform of Microphthalmia-Associated Transcription Factor and Its Role in Cell Proliferation

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-24 DOI: 10.1007/s10126-025-10435-y

Wenhao Yang, Jinhai Zhang, Yvlu Jiang, Chen Liu, Yijing Han, Yaqiong Liu, Xiaotong Wang

{"title":"Mechanism of Crassostrea gigas T-box Transcription Factor 2: Regulation by a Transcript Isoform of Microphthalmia-Associated Transcription Factor and Its Role in Cell Proliferation","authors":"Wenhao Yang, Jinhai Zhang, Yvlu Jiang, Chen Liu, Yijing Han, Yaqiong Liu, Xiaotong Wang","doi":"10.1007/s10126-025-10435-y","DOIUrl":"10.1007/s10126-025-10435-y","url":null,"abstract":"<div>T-box transcription factor 2 (TBX2) plays a critical role in various biological processes, including cell cycle regulation, malignant transformation, and regulating melanogenesis. In our previous study, we identified a Crassostrea gigas TBX2 (CgTBX2) and investigated its regulatory role in melanin production in oysters. Here, the mechanism of CgTBX2 in regulating cell proliferation was investigated. First, we found that CgTBX2 promoted the proliferation of mouse melanoma (B16F10) cells. CgMITF-X3, a 1347 bp transcript isoform of MITF from C. gigas, was then cloned and it was also found to promote cell proliferation. Co-transfection of CgTBX2 and CgMITF-X3 into B16F10 cells had a synergistic effect on cell proliferation, suggesting that CgMITF-X3 enhanced the function of CgTBX2 in promoting cell proliferation. CgMITF-X3 promoted the transcriptional activation of CgTBX2 by directly binding to the TCTCACGCGG sequence in the CgTBX2 promoter region. In addition, CgTBX2 and CgMITF-X3 proteins were co-located in the nucleus, indicating that these two proteins may perform a certain function collectively in the nucleus. Taken together, our findings revealed that CgTBX2 is directly activated by CgMITF-X3 at the transcriptional level, and both CgTBX2 and CgMITF-X3 facilitate cell proliferation.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Microsatellite Multiplex PCRs for Triploidy Verification and Genetic Diversity Assessment in the Pacific Oyster, Crassostrea gigas

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-21 DOI: 10.1007/s10126-025-10432-1

Huilin Zheng, Yuanxin Liang, Geng Cheng, Jianmin Zhou, Wenlong Bi, Hong Hu, Qi Li

{"title":"Optimization of Microsatellite Multiplex PCRs for Triploidy Verification and Genetic Diversity Assessment in the Pacific Oyster, Crassostrea gigas","authors":"Huilin Zheng, Yuanxin Liang, Geng Cheng, Jianmin Zhou, Wenlong Bi, Hong Hu, Qi Li","doi":"10.1007/s10126-025-10432-1","DOIUrl":"10.1007/s10126-025-10432-1","url":null,"abstract":"<div>The ploidy detection is crucial for the oyster industry. The objective of this study was to develop a method that verifies ploidy of the triploid Pacific oyster Crassostrea gigas by analyzing the diversity of triploid through microsatellite multiplex PCRs using fluorescent universal primers. We developed four information-rich multiplex PCR panels, comprising a total of 12 genomic microsatellites located in the genome of the C. gigas, distributed across seven chromosomes with an average of 14 alleles per locus. Each panel used M13(-21) primers labeled with specific fluorochrome dyes, and the forward primers for each locus were appended with M13(-21) sequences. We validated the approach to infer ploidy using flow cytometry as a reference, finding > 95% agreement between these methods, and demonstrated its potential utility to infer aneuploidy. Genotyping of 496 triploid samples from eight populations yielded 10 or more alleles per locus in 99.63% of samples in a single capillary electrophoresis. The correct assignment of triploidy depends on the number of markers with three unique allele fragments (MNM). Using semi-strict criteria of three unique alleles at one or more loci, the detection accuracy rate was 95.26% for triploids. Using the strict criteria of three unique alleles at two or more loci, the detection accuracy rate was 98.34%. Populations with reduced genetic diversity due to selective breeding were better suited for the semistrict criterion, maximizing triploid detection. And cultured populations were more suitable for evaluation using the strict criteria, which effectively reduced false-positive diploid assignment and increased triploid detection accuracy. The markers developed in this study were highly polymorphic and effective for assessing genetic diversity and distinguishing populations, providing a reliable tool for triploid detection and analysis in oyster breeding.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-20 DOI: 10.1007/s10126-025-10423-2

Shuo Li, Yuyan Liu, Qian Wang, Zhiwen Zhu, Weijing Li, Chen Li, Fan Fei, Baoliang Liu, Changwei Shao

{"title":"Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)","authors":"Shuo Li, Yuyan Liu, Qian Wang, Zhiwen Zhu, Weijing Li, Chen Li, Fan Fei, Baoliang Liu, Changwei Shao","doi":"10.1007/s10126-025-10423-2","DOIUrl":"10.1007/s10126-025-10423-2","url":null,"abstract":"<div>Flow velocity is a critical environmental factor influencing the growth, energy metabolism, and physiological health of aquaculture species. This study investigated the physiological and molecular responses of spotted sea bass (Lateolabrax maculatus) under experimental conditions simulating flow velocities typical of land-based recirculating aquaculture systems (RAS) and deep-sea cage systems. High flow velocities (HFV, 0.35–0.65 body lengths per second [BL/s]) enhanced growth performance compared to low flow velocity (LFV, 2.28–2.85 BL/s) conditions. Histological analysis revealed reduced hepatic lipid accumulation under HFV, while LFV promoted lipid storage. Serum analyses showed elevated antioxidant enzyme activity in the LFV group but higher oxidative stress markers in the HFV group. Transcriptomic profiling identified foxo3 as a key regulatory hub orchestrating metabolic and oxidative stress adaptations. Genes associated with oxidative damage repair, lipid catabolism, and glucose metabolism were significantly enriched under hydrodynamic stress. Enrichment of the FoxO signaling pathway highlighted its central role in mediating oxidative stress mitigation and energy mobilization. These findings demonstrate the dual effects of flow velocity, where higher velocities promote growth and metabolic activity at the cost of oxidative stress, and lower velocities conserve energy while maintaining oxidative stability. Tailored flow velocity conditions can optimize fish welfare and productivity across aquaculture systems. Future studies should investigate the systemic effects of hydrodynamic stress using multi-omics approaches to advance sustainable aquaculture practices.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia-induced changes in the gill and hepatopancreatic bacterial communities of the ark shell Anadara kagoshimensis

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-20 DOI: 10.1007/s10126-025-10430-3

Yukino Mizutani, Ryo Orita, Kei Kimura, Daisuke Funabara

{"title":"Hypoxia-induced changes in the gill and hepatopancreatic bacterial communities of the ark shell Anadara kagoshimensis","authors":"Yukino Mizutani, Ryo Orita, Kei Kimura, Daisuke Funabara","doi":"10.1007/s10126-025-10430-3","DOIUrl":"10.1007/s10126-025-10430-3","url":null,"abstract":"<div>Coastal hypoxia is an increasing environmental concern affecting marine ecosystems globally, particularly impacting benthic organisms such as bivalves. Although previous studies focused on the physiological responses of bivalves to hypoxic stress, the role of resident bacteria in the host response to hypoxia remains poorly understood. This study investigated changes in the resident bacterial communities in the gills and hepatopancreatic tissues of the ark shell (Anadara kagoshimensis) under hypoxic conditions. Specimens were assigned to three treatment groups: untreated control, hypoxia, and hypoxia with chloramphenicol supplementation (5.0 mg/L). After 3 days, specimens exposed to hypoxia exhibited black precipitation in the culture water, whereas antibiotic treatment reduced these effects. Amplicon sequencing revealed distinct bacterial communities between the tissues, with Arcobacteraceae and Alkalispirochaetaceae dominating in the gills and Metamycoplasmataceae being predominant in the hepatopancreas. The hepatopancreas displayed greater bacterial community changes than the gills under hypoxic conditions, including an increase in the abundance of Metamycoplasmataceae. The predicted metabolic functions suggested that these bacteria contribute to iron sulfide precipitation through sulfate reduction and iron respiration. The antibiotic-treated group displayed bacterial communities more similar to those of the control group, confirming the effectiveness of chloramphenicol in suppressing bacterial changes under hypoxia. This study provided new insights into tissue-specific bacterial responses to hypoxia in A. kagoshimensis and highlighted the potential role of Metamycoplasmataceae in the bivalve’s response to hypoxic stress.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Hepcidin Isoform Jd-Hep from the Sin Croaker Johnius dussumieri (Cuvier, 1830): Recombinant Expression and Insights into the Antibacterial Property and Modes of Action

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-19 DOI: 10.1007/s10126-025-10426-z

M. V. Anju, K. Archana, S. Muhammed Musthafa, V. V. Anooja, P. P. Athira, S. Neelima, M. Dhaneesha, T. P. Sajeevan, I. S. Bright Singh, Rosamma Philip

{"title":"A Novel Hepcidin Isoform Jd-Hep from the Sin Croaker Johnius dussumieri (Cuvier, 1830): Recombinant Expression and Insights into the Antibacterial Property and Modes of Action","authors":"M. V. Anju, K. Archana, S. Muhammed Musthafa, V. V. Anooja, P. P. Athira, S. Neelima, M. Dhaneesha, T. P. Sajeevan, I. S. Bright Singh, Rosamma Philip","doi":"10.1007/s10126-025-10426-z","DOIUrl":"10.1007/s10126-025-10426-z","url":null,"abstract":"<div>Hepcidin is a cysteine-rich antimicrobial peptide that plays an important role in fish immunity. In the current study, we report a novel isoform of hepcidin (Jd-Hep) from Sin croaker, Johnius dussumieri, with an open reading frame (ORF) of 258 nucleotide bases that encodes 85 amino acids containing a signal peptide (24 amino acids), a prodomain (35 amino acids) and a biologically active mature peptide (26 amino acids). Phylogenetic tree analysis showed that J. dussumieri hepcidin belonged to the HAMP2 cluster of hepcidin. The tissue distribution showed that the expression of hepcidin was highest in the liver in wild-caught J. dussumieri. The mature peptide mJd-Hep was recombinantly expressed in a prokaryotic host, E. coli Rosetta-gami™B (DE3) pLysS cells, and the peptide was isolated and purified. The recombinant peptide, rJd-Hep, exhibited notable antibacterial activity against aquatic pathogens such as Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio proteolyticus, and Vibrio fluvialis. The mode of action of the peptide was proven to be membrane-based (pore formation and depolarization). The rJd-Hep was found to be non-hemolytic to hRBCs and non-cytotoxic to the mammalian cell line. The peptide showed 85% growth inhibition of cancer cell line, MCF-7. These findings expand our knowledge of the potential application of hepcidin in aquaculture as a therapeutic agent.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10426-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Striped Bass (Morone saxatilis) and White Bass (M. chrysops) Splenic Transcriptome Following Streptococcus iniae Infection

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-17 DOI: 10.1007/s10126-025-10431-2

Linnea K. Andersen, Jason W. Abernathy, Bradley D. Farmer, Miles D. Lange, Nithin M. Sankappa, Matthew E. McEntire, Steven D. Rawles

{"title":"Analysis of Striped Bass (Morone saxatilis) and White Bass (M. chrysops) Splenic Transcriptome Following Streptococcus iniae Infection","authors":"Linnea K. Andersen, Jason W. Abernathy, Bradley D. Farmer, Miles D. Lange, Nithin M. Sankappa, Matthew E. McEntire, Steven D. Rawles","doi":"10.1007/s10126-025-10431-2","DOIUrl":"10.1007/s10126-025-10431-2","url":null,"abstract":"<div>Streptococcal disease results in major mortality events of both marine and freshwater fishes worldwide. Streptococcus iniae is among the prominent causative bacterial strains as it has been found to cause a higher incidence of mortality and act as a zoonotic pathogen. Here, we examine the susceptibility of two important aquaculture species in the USA, striped bass (Morone saxatilis) and white bass (Morone chrysops) to S. iniae. A high incidence of mortality was observed in both species, although striped bass succumbed more rapidly than white bass. Spleen gene expression at three time points following infection was analyzed to further elucidate the mechanisms underlying these observations. The down-regulation of gene transcripts associated with pathogen detection (tlr1, tlr8, tlr9), antigen processing (cd74a), immune cell recruitment and migration (ccr6b, ccr7), macrophage function (csf1ra), T-cell signaling, and NF-kB activation (card11, fyna, tirap) was detected in both species. These findings potentially indicate impairment in these critical early immune system processes such that both species were ultimately highly susceptible to S. iniae infection despite the detected up-regulation of transcripts typically associated with effective immune response, such as cytokines (il1β, il8, il12b2, il17rc, tnfα) and hepcidins (hamp, hamp2). The presented results collectively identify several candidate genes and associated pathways for further investigation to characterize the vulnerability of striped bass and white bass to S. iniae and that may be considered for selective breeding efforts, biotechnological intervention, and/or exploitation in the development of vaccines and alternative treatments.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid- and Multivariate-Based Analyses to Determine Cell Response to pH Variations and Buffer Composition

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-15 DOI: 10.1007/s10126-025-10421-4

Ricardo F. S. Pereira, Carla C. C. R. de Carvalho

{"title":"Lipid- and Multivariate-Based Analyses to Determine Cell Response to pH Variations and Buffer Composition","authors":"Ricardo F. S. Pereira, Carla C. C. R. de Carvalho","doi":"10.1007/s10126-025-10421-4","DOIUrl":"10.1007/s10126-025-10421-4","url":null,"abstract":"<div>During marine bioprocess development, pH control is of paramount importance. In shake flasks, aerobic fermentations usually have no pH control except from the buffering effect provided by buffers in the medium. However, the use of traditional buffers, such as phosphate buffer, can lead to the precipitation of medium components. Good’s buffers may be a sound alternative. Using Serratia rubidaea cells and their production of prodigiosin as model system, several Good’s buffers were tested and compared to phosphate buffer. Lipidomics analysis in conjugation with statistical multivariate analysis was performed to determine the cellular response to pH variations and buffer composition. Biomass productivity was similar when using the different buffers, but prodigiosin production was highly influenced and was highest with MES buffer at pH 5.5, reaching 249.8 mg/L, which corresponds to 43.7 mg/gbiomass. At pH 7.0, the best results were achieved with EPPS, HEPES and TRIS buffer, being these good substitutes for phosphate buffer in marine medium. The results also show that cells adapted the fatty acid composition of their membranes as response to the buffering species present in the growth medium. This is a clear indication that the buffer composition should not be disregarded when developing a bioprocess.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10421-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Efficient Method for Enrichment and In Vitro Propagation of Muscle Stem Cells Derived from Black Sea Bream (Acanthopagrus schlegelii) Skeletal Muscle

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-15 DOI: 10.1007/s10126-025-10428-x

Dan Hee Han, Seung Pyo Gong

{"title":"An Efficient Method for Enrichment and In Vitro Propagation of Muscle Stem Cells Derived from Black Sea Bream (Acanthopagrus schlegelii) Skeletal Muscle","authors":"Dan Hee Han, Seung Pyo Gong","doi":"10.1007/s10126-025-10428-x","DOIUrl":"10.1007/s10126-025-10428-x","url":null,"abstract":"<div>Muscle stem cells (MSCs) play a crucial role in muscle growth, repair, and regeneration, offering potential applications in cell-mediated therapy, tissue engineering, and alternative food production. Despite significant advancements in isolating and enriching MSCs from mammalian tissues, research on fish MSCs remains limited. This study aimed to establish an optimized protocol for isolating, enriching, and propagating black sea bream (Acanthopagrus schlegelii) MSCs for potential biotechnological applications. Skeletal muscle tissues were enzymatically dissociated using various enzymes, with collagenase type II and pronase identified as the most effective combination for cell isolation and tissue debris removal. Differential plating (DP) on collagen type I effectively enriched MSCs, as evidenced by a significant increase in Pax7 expression in non-adhesive cells. Among several adhesion substrates tested, Matrigel-coated dishes best supported the maintenance and differentiation potential of enriched MSCs, enabling robust myotube formation. To mitigate the high cost of Matrigel, cells were transitioned to laminin- or gelatin-coated dishes after the early passages. Notably, Matrigel-conditioned cells maintained their survival and differentiation capacities on these more cost-effective substrates. After long-term culture on gelatin-coated dishes, the cell lines were stably maintained for more than 25 passages, and their myogenic differentiation potentials were well preserved, with variations observed between the cell lines. These findings provide a foundational framework for the efficient isolation, enrichment, and culture of fish MSCs, contributing to the development of scalable and cost-effective protocols for their application in muscle biology and biotechnology.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive Benefits of Dietary Selenium Nanoparticles (SeNPs) in Asian Seabass (Lates calcarifer) Male Broodstock

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-12 DOI: 10.1007/s10126-025-10429-w

Saeed Keyvanshokooh, Amir Parviz Salati, Ahmad Ghasemi, Samira Nazemroaya, Hossein Houshmand, Mansour Torfi Mozanzadeh

{"title":"Reproductive Benefits of Dietary Selenium Nanoparticles (SeNPs) in Asian Seabass (Lates calcarifer) Male Broodstock","authors":"Saeed Keyvanshokooh, Amir Parviz Salati, Ahmad Ghasemi, Samira Nazemroaya, Hossein Houshmand, Mansour Torfi Mozanzadeh","doi":"10.1007/s10126-025-10429-w","DOIUrl":"10.1007/s10126-025-10429-w","url":null,"abstract":"<div>Selenium (Se), a critical trace element for human and animal health, is essential for numerous physiological processes, including antioxidant defense, immune function, and reproduction. This study investigated the effects of dietary selenium nanoparticles (SeNPs) at 2 mg/kg on the reproductive performance, Se deposition, antioxidant status, and gene expression in male Asian seabass (Lates calcarifer). Over 120 days before spawning, the male broodfish were fed either a control diet (CD) or a SeNP-supplemented diet (SeD) in triplicate tanks per group. The SeD group exhibited significantly higher gonadosomatic index (GSI), fertilization rate, and hatching rate, along with lower rates of abnormal embryogenesis compared to the CD group (P < 0.05). Selenium accumulation was significantly elevated in the liver and testis tissues of the SeD group, corresponding with increased expression of the hepatic selenop gene. Reproduction-related genes, including ar, p450scc, and cdk1, were significantly upregulated in the testis of the SeD group, indicating enhanced reproductive processes. Antioxidant-related genes, including catalase (cat), superoxide dismutase (sod), and glutathione-s-transferase (gst) were also upregulated in both liver and testis tissues, while catalase activity increased in the testis. In terms of sex steroid hormones, the SeD group displayed significantly higher serum levels of testosterone and progesterone (P < 0.05), suggesting enhanced steroidogenesis. The absence of adverse effects further demonstrates the efficacy and safety of SeNP supplementation at 2 mg/kg. This study highlights the potential of SeNPs to enhance reproductive performance, regulate molecular pathways, and optimize broodstock management in aquaculture.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Chromatin Accessibility and Transcriptional Regulation in the Eyes of Red Tilapia (Oreochromis sp.) in Response to Wintering Stress

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2025-02-12 DOI: 10.1007/s10126-025-10424-1

Ying Bai, Gan Yang, Tongde Liu, Fuyan Chen, Junhong Xia

{"title":"Dynamic Chromatin Accessibility and Transcriptional Regulation in the Eyes of Red Tilapia (Oreochromis sp.) in Response to Wintering Stress","authors":"Ying Bai, Gan Yang, Tongde Liu, Fuyan Chen, Junhong Xia","doi":"10.1007/s10126-025-10424-1","DOIUrl":"10.1007/s10126-025-10424-1","url":null,"abstract":"<div>During wintering, red tilapia may develop variable black spots on their bodies, significantly reducing their market value. Understanding the mechanisms driving this phenomenon is essential for molecular improvements in body color. In this study, we investigated chromatin accessibility landscapes in the eyes of red tilapia with two distinct phenotypes (normal pure red and black spot) under wintering stress using ATAC-seq and RNA-seq analyses. We observed that approximately 32.7% of chromatin accessibility peaks were located in promoter regions, followed by intergenic regions (32.4%) and intronic regions (26.7%). One thousand two hundred twenty-nine differentially accessible regions (DARs) and 1448 differentially expressed genes (DEGs) were identified between the RS and DS groups. Notably, DEGs associated with melanin synthesis, including tyrp1, tyr, tyrp1b, pmela, slc24a5, and mlph, were significantly upregulated in the DS group, which aligns with the observed 1.85-fold increase in melanin content, compared to the RS group. 92 DEGs were associated with significant changes in chromatin accessibility between groups (R2 = 0.8059; p < 0.0001), indicating potential regulatory relationships. Interestingly, 23.92% of the DARs were located on the chromosome 3. Specifically, a 2.5-fold difference in average peak height on LG3: 11,215,273–11,217,225 were observed between DS and RS tilapia. In the region, transcription factors including HSF1 and HSF2 were identified as key regulators of chromatin structure and gene expression under wintering stress. Our findings reveal that dynamic chromatin accessibility in the eyes of red tilapia facilitates adaptation to wintering stress by regulating visual signaling, melanin production, and downstream pigmentation.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0