Marine Biotechnology最新文献

{"title":"Reduced Vitellogenin Expression is Associated with Impaired Oogenesis in Female Triploid Pacific Oysters (Crassostrea gigas)","authors":"Chunxue Zheng,&nbsp;Yaru Zhou,&nbsp;Hong Yu,&nbsp;Qi Li","doi":"10.1007/s10126-026-10616-3","DOIUrl":"10.1007/s10126-026-10616-3","url":null,"abstract":"<div>\u0000 \u0000 <p>Triploidy is widely applied in oyster aquaculture because of its association with reduced gametogenesis, yet female triploid Pacific oyster (<i>Crassostrea gigas</i>) exhibit pronounced variability in ovarian development. The mechanisms underlying ovarian impairment in triploid females remain poorly understood. In this study, we investigated the role of vitellogenin (<i>VTG</i>) in oogenesis and gonadal development by comparing diploid females with β triploid females exhibiting severe gonadal arrest. Quantitative PCR and in situ hybridization analyses revealed that the expression of <i>VTG</i> was significantly reduced in the gonads of β triploid females compared with diploids, with <i>VTG</i> mRNA predominantly localized in the follicle cells. Functional suppression of <i>VTG</i> expression in diploid females using RNA interference resulted in abnormal germ cell development, reduced gonadal coverage area, and impaired oocyte maturation, demonstrating that adequate <i>VTG</i> expression is important for normal ovarian development. Targeted steroid profiling by LC-MS/MS demonstrated significant alterations in the gonadal endocrine environment of triploids, including elevated testosterone levels. In vivo hormone administration further showed that 17β-estradiol significantly upregulated <i>VTG</i> expression, while testosterone markedly suppressed it. Together, these findings indicate that reduced <i>VTG</i> expression, potentially arising from disrupted endocrine regulation, is closely associated with impaired oogenesis in female triploid oysters. This study provides mechanistic insight into triploid-associated ovarian dysgenesis and contributes to a better understanding of reproductive regulation in bivalve aquaculture species.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal Transcriptomic Atlas Reveals Conserved and Sex-Biased Regulatory Networks in Black-Spotted Croaker (Protonibea diacanthus)","authors":"Cheng Peng,&nbsp;Jiahui Chen,&nbsp;Qiyan Pan,&nbsp;Jingpeng Hou,&nbsp;Xiaopiao Zhong,&nbsp;Yong Zhang,&nbsp;Shijia Hu","doi":"10.1007/s10126-026-10613-6","DOIUrl":"10.1007/s10126-026-10613-6","url":null,"abstract":"<div>\u0000 \u0000 <p>The black-spotted croaker (<i>Protonibea diacanthus</i>) is a commercially important marine fish, in which males possess swimbladders of superior market value. To establish a molecular foundation for all-male breeding, this study presents the first comprehensive gonadal transcriptomic analysis of this species. RNA sequencing of ovary and testis identified 8,251 sex-biased differentially expressed genes. Functional enrichment analyses revealed their significant involvement in key reproductive pathways, including ovarian steroidogenesis and oocyte meiosis. Our analysis validated the sexually dimorphic expression of conserved sex-related genes. Testis-biased expression was confirmed for regulators and enzymes including <i>dmrt1</i>, <i>amh</i>, <i>sox9</i>, <i>sycp3</i>, and genes in the androgen synthesis pathway (<i>star</i>, <i>cyp11a1</i>, <i>cyp11c1</i>, <i>hsd3b1</i>). Conversely, ovary-biased expression was observed for pivotal female development genes such as <i>cyp19a1a</i>, <i>foxl2</i>, <i>hsd17b1</i>, <i>gdf9</i>, and <i>bmp15</i>. Protein-protein interaction network analysis further identified Dmrt1 and Cyp19a1a as central hubs within the sex-regulatory network. These findings provide a foundational transcriptomic atlas and identify candidate genes for future functional studies aimed at all-male production, but substantial downstream validation is still required to enhance the commercial production of <i>P. diacanthus</i>.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Seaweed-Derived Polysaccharides in Biocrust Technology: Ultrasound-Assisted Fucoidan Extraction from Laminaria digitata","authors":"Ivan Savić,&nbsp;Ivana Savić Gajić,&nbsp;Tamara Palanački Malešević,&nbsp;Ivana Mihalj Lubaščik,&nbsp;Jelena Vučković,&nbsp;Zorica Svirčev","doi":"10.1007/s10126-026-10606-5","DOIUrl":"10.1007/s10126-026-10606-5","url":null,"abstract":"<div>\u0000 \u0000 <p>Fucoidan, a polysaccharide derived from brown seaweed, exhibits significant potential as a natural ingredient with a multitude of health benefits due to its non-toxic properties, biodegradability, and extensive range of applications. This study aimed to optimize the extraction of fucoidan from brown seaweed (<i>Laminaria digitata</i>) and its application as a structural support for enhanced biocrust establishment. Ultrasound-assisted extraction of fucoidan was carried out using a 0.1 M hydrochloric acid. The extraction conditions were optimized using a Box–Behnken design with three parameters. The extraction time of 6.3 min, extraction temperature of 50 °C, and a liquid-to-solid ratio of 15 mL/g were obtained as the optimal conditions. Under these optimal conditions, the yield of fucoidan was 5.20 ± 0.32 g/100 g dry matter. The proposed isolation procedure proved to be more effective compared to conventional extraction where the fucoidan yield was 2.92 ± 0.11 g/100 g dry matter. The viscosity-average molecular weight of fucoidan isolated using ultrasound-assisted extraction was approximately 289.01 kDa with a sulfate content of 19%, whereas conventional extraction yielded a higher molecular weight (420.25 kDa) but lower sulfate content (17%). The results of the hydration properties and biocrust growth suggest that fucoidan could be used to significantly enhance cyanobacterial biomass production and provide structural support for development of the biocrusts. This highlights its potential as a practical tool for accelerating restoration of degraded soils and the preventing erosion.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Genes Encoding for Enzyme-associated Function in Bacillus paranthracis B1-S Strain Isolated from the Deep-sea environment of Andaman Sea","authors":"Abhay B. Fulke,&nbsp;Shreya Bhanushali,&nbsp;Eisha Patrikar,&nbsp;Nilkanth Sharma","doi":"10.1007/s10126-026-10611-8","DOIUrl":"10.1007/s10126-026-10611-8","url":null,"abstract":"<div><p>The ecological distribution of <i>Bacillus paranthracis</i>, primarily reported in terrestrial and clinical settings, remains unexplored in extreme marine ecosystems. This study addresses the knowledge gap by reporting the first isolation of strain B1-S, from the deep-sea sediment at a depth of 3,581 m in the Andaman Sea. The strain demonstrated extracellular production of protease, lipase, and DNase with enzyme activity indices of 1.08, 1.25 and 1.07, respectively. Genome sequencing revealed a 6.39 Mbp genome with 35.92% G + C content, high completeness (99.92%), and contamination (4%). Annotation identified multiple genes encoding hydrolytic enzymes, including proteases, lipases, and DNases, as well as carboxylase and decarboxylase genes, corroborating the observed enzymatic activity. The absence of genes encoding amylase, phosphatase, cellulase, and pectinase matched the negative in vitro assay results. This alignment between genomic and phenotypic data supports the organism’s metabolic capabilities for survival in a nutrient-limited deep-sea habitat. The findings highlight <i>B. paranthracis</i> B1-S may have potential for enzyme-based industrial applications, thereby contributing to the growing field of marine bioprospecting and the blue economy, thereby expanding the knowledge of known ecological niche.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alt text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotic Dietary Supplementation with Sonicated Shewanella sp. SpPdp11 Improves Intestinal Status in Juvenile Senegalese Sole (Solea senegalensis)","authors":"I.M Cerezo,&nbsp;B. Delgado-Martín,&nbsp;A.J Vizcaíno,&nbsp;A. Galafat,&nbsp;F.J Alarcón-López,&nbsp;I. García de la Banda,&nbsp;A. Hernández de Rojas,&nbsp;L. Díaz,&nbsp;M.C Balebona,&nbsp;S.T Tapia-Paniagua,&nbsp;M. A. Moriñigo","doi":"10.1007/s10126-026-10608-3","DOIUrl":"10.1007/s10126-026-10608-3","url":null,"abstract":"<div>\u0000 \u0000 <p>The use of non-viable microbial preparations (postbiotics) is emerging as a safe alternative to probiotics in aquaculture. In this study, we investigated the impact of dietary inclusion of sonicated cells of the marine bacterium <i>Shewanella</i> sp. Pdp11 (SpPdp11) on the intestinal health of juvenile Senegalese sole (<i>Solea senegalensis</i>). Fish were fed a control diet or a diet supplemented with SpPdp11 postbiotic for 45 days, and their intestinal status was assessed using histological, transcriptomic, and microbiota analyses. Postbiotic supplementation improved intestinal morphology by widening villi and reducing lamina propria and epithelial height, suggesting enhanced barrier function. RNA-seq analysis revealed downregulation of inflammatory pathways, including ECM receptor interaction and PPAR signaling, together with activation of bile acid biosynthesis. 16 S rRNA profiling showed a marked shift in microbial composition, with decreased prevalence of opportunistic taxa such as <i>Vibrio</i> and <i>Stenotrophomona</i>s and enrichment of beneficial genera, including <i>Acinetobacter</i>,<i> Pseudomonas</i>,<i> and Malacoplasma.</i> These results provide evidence that marine-derived postbiotics can modulate host physiology and gut microbial communities without the risks associated with live probiotics. Sonicated SpPdp11 represents a promising biotechnological feed additive to support intestinal health, reduce inflammation, and promote sustainable aquaculture practices.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13079515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Epigenetic Regulation Links Bacterial Signaling to Growth and Reproductive Development in the Green Macroalga Ulva compressa (Chlorophyta)","authors":"Janine F. M. Otto,&nbsp;Hermann Holbl,&nbsp;Nico Ueberschaar,&nbsp;Georg Pohnert,&nbsp;Thomas Wichard","doi":"10.1007/s10126-026-10605-6","DOIUrl":"10.1007/s10126-026-10605-6","url":null,"abstract":"","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147662092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue and Sex-Specific Transcriptomic Signatures Underlying Aggression behavior of Chinese Mitten Crab (Eriocheir sinensis)","authors":"Jinliang Hou,&nbsp;Wenqi Zhao,&nbsp;Yongzhi Zhang,&nbsp;Xin Hou,&nbsp;Xiaowen Chen,&nbsp;Jun Wang,&nbsp;Chenghui Wang","doi":"10.1007/s10126-026-10607-4","DOIUrl":"10.1007/s10126-026-10607-4","url":null,"abstract":"<div><p>Aggressive behavior is a critical constraint on sustainable crustacean aquaculture, causing cannibalism and yield loss. However, in the economically important Chinese mitten crab (<i>Eriocheir sinensis</i>), the molecular mechanisms underlying aggression responsiveness, particularly sex-specific regulation, remain poorly understood. Here, we used a standardized behavioral assay to quantify reactive aggression across three developmental stages and classified the quantified crabs into high-, moderate-, and low-aggression groups. Biochemical and transcriptomic profiling of the thoracic ganglion and the first pereiopod muscle were investigated to decode the mechanisms of aggression. High aggression was associated with significantly elevated catalase (CAT) activity and malondialdehyde (MDA) content, indicating increased oxidative stress that may reflect both the energetic demand of aggressive responses and the acute stress induced by the assay. Transcriptomic analyses showed that the upregulated genes in high-aggression crabs were enriched in neurotransmission and signal transduction pathways including dopaminergic, GABAergic, serotonergic, cAMP, and calcium signaling, which are consistent with altered neuromodulatory and signal-transduction capacity associated with high reactive aggression. Notably, significant enrichment of these neurotransmitter-related pathways was observed only in males, suggesting distinct sex-dependent adaptive strategies for aggressive behavior. Moreover, upregulated genes identified in the first pereiopod muscle of both females and males were mainly involved in metabolic processes, signal transduction, and immune defense pathways, indicating conserved functional roles across sexes. This study provides an integrative neurophysiological and transcriptomic framework for understanding the stimulus-induced aggressive response in Chinese mitten crab, highlighting oxidative trade-offs, tissue-specific regulation, and sex-dependent molecular strategies. These would help to conduct selective breeding and behavioral control in crustacean aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Probiotic and Enzyme Supplementation Enhances Growth Performance, Digestive Function, and Immune Response in Penaeus vannamei","authors":"Rachman Syah,&nbsp; Makmur,&nbsp;Bunga Rante Tampangallo,&nbsp;Sri Redjeki Hesti Mulyaningrum,&nbsp;Ince Ayu Khaerana Kadriah,&nbsp;Mat Fahrur,&nbsp;Muhammad Chaidir Undu,&nbsp;Imam Taukhid,&nbsp;Brata Pantjara,&nbsp;Muhammad Roil Bilad","doi":"10.1007/s10126-026-10603-8","DOIUrl":"10.1007/s10126-026-10603-8","url":null,"abstract":"<div>\u0000 \u0000 <p>This study assesses the impact of probiotic and enzyme supplementation on the growth, health, digestive enzyme activity, immune response, and nutritional composition of Pacific white shrimp (<i>Penaeus vannamei</i>). As sustainable aquaculture practices become increasingly important, functional feed additives like probiotics and enzymes are considered viable alternatives to antibiotics. The experiment was conducted in controlled indoor conditions using a completely randomized design with four treatments: a control group, a probiotic-phytobiotic supplement (ADX), a multienzyme complex (ZZ), and a combination of both (ADX + ZZ). These additives were incorporated into commercial feed throughout the trial period. Results showed that ZZ and ADX + ZZ improved growth performance, and ADX + ZZ yielded the lowest feed conversion ratio. Digestive enzyme responses were additive-specific: ADX increased protease and amylase activities, whereas ZZ and ADX + ZZ increased lipase activity, with no clear change in cellulase. Survival did not differ among treatments, while immune indices varied, with higher total hemocyte counts in ZZ and higher Prophenoloxidase activity in ADX + ZZ. Whole-body proximate composition and amino acid profiles were improved in supplemented groups, with the highest total amino acids observed in ADX + ZZ. Water quality parameters remained within acceptable limits across all treatments, supporting the environmental compatibility of these additives. Overall, probiotic–enzyme strategies can enhance <i>P. vannamei</i> performance and feed utilization; further work should validate these effects at farm scale and examine long-term and microbiota-mediated responses.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS Profiling, Cytotoxicity and Antiviral Activity of Caulerpa lentillifera and C. macrodisca from Northern Coastal Waters of Borneo Island, Eastern Malaysia","authors":"Mohammad Tamrin Mohamad Lal,&nbsp;Elden Sai Long Chang,&nbsp;Zy Chee Wong,&nbsp;Leong Seng Lim,&nbsp;Muhammad Dawood Shah,&nbsp;Wahidatul Husna Zuldin,&nbsp;Gilbert Ringgit,&nbsp;Nur Azreen Arbi,&nbsp;Ahmad Hazim Abdul Aziz,&nbsp;Abdul Aziz Jaziri,&nbsp;Asep Awaludin Prihanto,&nbsp;Attabik Mukhamad Amrillah,&nbsp;Muhamad Gustilatov,&nbsp;Hiroaki Saito,&nbsp;Motohiko Sano","doi":"10.1007/s10126-026-10604-7","DOIUrl":"10.1007/s10126-026-10604-7","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioactive compounds derived from marine sources have gained global attention in aquaculture disease control due to their novelty and promising medicinal value. However, marine algae as an alternative source remain underexplored particularly along the Borneo coast. In this study, marine algae specifically <i>Caulerpa lentillifera</i> and <i>C. macrodisca</i> were investigated on their toxicity and antiviral activity as well as bioactive profiling. The cytotoxicity was evaluated using five fish cell lines. The antiviral activity was assessed against infectious hematopoietic necrosis virus (IHNV). Results showed that both <i>Caulerpa species</i> exhibited moderate cytotoxicity on E-11 and EPC cells after six and 14 days, respectively. The antiviral assay revealed higher viral titer reduction by <i>C. macrodisca</i> (1.80 log₁₀ TCID₅₀ mL⁻¹; 98.38%) compared to <i>C. lentillifera</i> (0.46 log₁₀ TCID₅₀ mL⁻¹; 65.19%) after 10 days. GC-MS identified multiple potential bioactive metabolites, including two potential antiviral compounds from <i>C. lentillifera</i> and five potential antiviral compounds from <i>C. macrodisca</i>. These findings highlight the potential of Bornean <i>Caulerpa</i> species as a novel source of marine-derived antiviral metabolites for sustainable fish health management in aquaculture.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-026-10604-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147519773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct Gene Expression Patterns Reveal the Acclimatization Strategies To High-Salinity Stress in Gills of Mozambique Tilapia (Oreochromis mossambicus)","authors":"Jinghong He,&nbsp;Na Xue,&nbsp;Hancheng Bao,&nbsp;Boyuan Wang,&nbsp;Yuan Yao,&nbsp;Yun Li,&nbsp;Xin Qi,&nbsp;Qinfeng Gao,&nbsp;Yuan Tian","doi":"10.1007/s10126-026-10592-8","DOIUrl":"10.1007/s10126-026-10592-8","url":null,"abstract":"<div>\u0000 \u0000 <p>Recently, water salinities are dramatically increased and largely exceed the natural thresholds. It poses the severe osmotic stresses on fishes. Hence, much attention has been focused on the physiological adaptation of fishes to salinity ranging from 0 to 38‰. However, research on the adaptation to high-salinity environments remains limited. In the present study, 18 RNA-Seq datasets were generated from gills of <i>O. mossambicus</i> under acute high-salinity stress, including 0‰, 30‰, 50‰, 70‰, 90‰ and 110‰. Through pairwise comparisons between each salinity treatment and the 0‰ (freshwater) group, we identified 773, 5,854, 10,852, 4,557, and 7,852 differentially expressed genes (DEGs), respectively. Based on the expression patterns, 3 stages were considered important for the salinity acclimatization: initial acclimatization to high-salinity stress (30‰), acclimatization to severely high-salinity stress (70‰), and acclimatization to extremely high-salinity stress (110‰). At 30‰, in addition to ion transporters, many genes associated with oxidative phosphorylation and purine metabolism also showed upregulated expression. It may suggest the energy metabolism reorganization responsible for high-salinity stress. At 70‰, numerous ion and amino acids transporters were fully activated to balance the osmotic pressures. When confronted with 110‰ salinity, a unique set of genes was activated to regulate sphingolipid metabolism and protect cells from endoplasmic reticulum (ER) stress, contributing to membrane integrity and protein stability. The study revealed the distinct acclimatization strategies to acute high-salinity stress in <i>O. mossambicus</i>, providing valuable insights into osmoregulation in euryhaline teleosts.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}