Marine Biotechnology最新文献

{"title":"Environmental Changes Drives the Transcriptome and Gene Regulation Plasticity During Sea Lice Infestation","authors":"Antonio Casuso,&nbsp;Valentina Valenzuela-Muñoz,&nbsp;Constanza Sáez-Vera,&nbsp;Cristian Gallardo-Escárate","doi":"10.1007/s10126-025-10459-4","DOIUrl":"10.1007/s10126-025-10459-4","url":null,"abstract":"<div><p>The sea louse, <i>Caligus rogercresseyi</i>, is one of the main concerns in the Chilean salmon industry. The free-living copepodid stage can recognize the host and initiate the parasitic phase, where environmental factors can shape the host recognition process. This study aimed to explore the ecological influence on the transcriptome of copepodids infesting Atlantic salmon experimentally exposed to different salinity and temperature (S/T) conditions. Herein, 200 salmon were infested with 35 copepodids per fish previously acclimatized to four S/T treatments: 32 and 26 PSU; 8 and 16°C. After 48 h of infestation, the attached copepodids from each experimental group were counted and digitalized for geometric morphometric analysis. Copepodids were then collected for RNA sequencing to analyze transcriptome modulation and gene regulation. Morphological changes in copepodids were mainly associated with temperature rather than salinity conditions. The transcriptome survey revealed molecular signatures related to salinity and temperature changes, where salinity drives the gene expression of copepodids. Notably, specific genes, such as those encoding cuticle proteins and trypsin-like kinases, were regulated by all three post-transcriptional mechanisms assessed: alternative splicing, miRNA, and gene fusion. The transcriptome analysis revealed that trypsin-like kinase genes exhibited upregulation and downregulation across the various S/T conditions. In contrast, cuticle protein genes were consistently downregulated in the 32 PSU/8°C, 26 PSU/8°C, and 26 PSU/16°C groups compared to the 32 PSU/16°C control. This suggests that the three post-transcriptional mechanisms may exert a combined influence on the expression of specific genes, potentially driven by salinity and temperature environmental conditions in sea lice biology.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sterilization of Channel Catfish (Ictalurus punctatus) via Overexpression of bax Gene Regulated by a Tet-off System in the Primordial Germ Cells","authors":"Zhi Ye,&nbsp;Ahmed Elaswad,&nbsp;Guyu Qin,&nbsp;Dongdong Zhang,&nbsp;Baofeng Su,&nbsp;Karim Khalil,&nbsp;Zhenkui Qin,&nbsp;Nermeen Y. Abass,&nbsp;Qi Cheng,&nbsp;Ramjie Odin,&nbsp;Khoi Vo,&nbsp;Nathan Backenstose,&nbsp;David Drescher,&nbsp;Mei Shang,&nbsp;Hanbo Li,&nbsp;Dan Zhang,&nbsp;William S. Bugg,&nbsp;Kamal Gosh,&nbsp;Rex A. Dunham","doi":"10.1007/s10126-025-10456-7","DOIUrl":"10.1007/s10126-025-10456-7","url":null,"abstract":"<div><p>Transgenic technologies have been used for genetic improvement of catfish performance with notable success. However, these developments are useless from a commercialization standpoint without extremely efficient confinement. Transgenic sterilization has the potential to accomplish 100% reproductive confinement and avoid genetic exchange between transgenic or domestic genotypes and wild populations. The present study reports a novel sterilization method for channel catfish by overexpressing the pro-apoptosis gene <i>bax</i>, specifically in the primordial germ cells, to inhibit their proliferation. Three transgenic constructs were electroporated into channel catfish one-cell embryos, including Nanos-nanos, Nanos-dnd, and Dazl-vasa. Transgene integration, gonad development, and sex ratio were evaluated in P₁ and F₁ generations. The transgene was successfully integrated into the channel catfish genome, with variable rates depending on each construct. Mosaicism of transgene integration was widely evident in the P₁ fish, as expected. All three constructs showed similar efficacy for sterilizing P₁ male channel catfish, with approximately half of all males showing little to no gonadal development, resulting in a significantly lower (<i>p</i> &lt; 0.05) gonadosomatic index (GSI) when compared to the control at four years of age. The same trend occurred but with lower efficacy in P₁ females, with approximately one-third showing little gonadal development at four years of age. This technology is potentially useful for generating sterile male fish, where the overexpression of the <i>bax</i> gene can lead to reduced or no gonadal development, presumably due to the death of primordial germ cells.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Environmentally Friendly Aquaculture Chamber Coatings on Enzyme Activities, Histology, and Transcriptome in the Liver of Larimichthys crocea","authors":"Xuan Xu,&nbsp;Huayu Song,&nbsp;Huicai Wu,&nbsp;Lu Zhang,&nbsp;Fengjun Lin,&nbsp;Chonghui Chen,&nbsp;Xiaoxu Zhang,&nbsp;Yiying Liu,&nbsp;Chao Li,&nbsp;Qiang Fu","doi":"10.1007/s10126-025-10453-w","DOIUrl":"10.1007/s10126-025-10453-w","url":null,"abstract":"<div><p>Aquaculture vessels have emerged as a sustainable alternative to traditional offshore aquaculture. However, the biological impacts of protective coatings used for vessel interiors are still poorly understood. This study assessed acute stress responses of <i>Larimichthys crocea</i> to epoxy-based aquaculture coatings using actual culture (1-fold) and high-exposure (80-fold) concentrations. Liver analyses included antioxidant enzymes, histopathology, and transcriptomics over 12–96 h. Firstly, the effect of the 80-fold concentration group on the activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) was more significant in the liver of <i>L. crocea</i> compared to the 1-fold concentration group. Similarly, histological observations revealed that the 80-fold concentration group produced more significant pathological changes in the liver than the 1-fold concentration group, including hepatocyte damage and vacuolization. Subsequently, through high-throughput sequencing, a total of 714.02 million clean reads were obtained, with 693.71 million of these reads successfully mapped onto the reference genome of <i>L. crocea</i>, identifying 13,709 differentially expressed genes (DEGs). KEGG pathway enrichment analysis showed that many DEGs following coating-treated were involved in protein processing in endoplasmic reticulum, oxidative phosphorylation, cytokine-cytokine receptor interaction, FoxO signaling pathway, and toll-like receptor signaling pathway. Finally, fifteen DEGs were selected for quantitative real-time PCR (qRT-PCR) analysis, and the results showed a significant correlation with RNA-seq results, verifying the reliability and accuracy of the high-throughput sequencing data. This study preliminarily revealed the stress responses induced by aquaculture vessel coatings in <i>L. crocea</i> and provided fundamental data into the scientific use of coatings on aquaculture vessels.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Characterization of Hepatocyte Line from Turbot (Scophthalmus maximus L.) and Its Application in the Study of Glucose Metabolism","authors":"Mingzhu Pan,&nbsp;Gaochan Qin,&nbsp;Jiahuan Liu,&nbsp;Mengxi Yang,&nbsp;Xinxin Li,&nbsp;Zhenhua Wu,&nbsp;Kangsen Mai,&nbsp;Wenbing Zhang","doi":"10.1007/s10126-025-10448-7","DOIUrl":"10.1007/s10126-025-10448-7","url":null,"abstract":"<div><p>The present study was to establish a hepatocyte line and investigate its role in glucose metabolism. A continuous cell line, THL (turbot hepatocyte line), was established from the liver tissue of turbot (<i>Scophthalmus maximus</i> L<i>.</i>). It has been successfully passaged more than 60 generations. The THL cells showed an epithelial-like morphology and the normal chromosome number was 44. Different methods were used to identify the hepatocytes. Periodic acid-Schiff (PAS) staining for THL cells was positive, and two key functional proteins of hepatocytes, cytokeratin- 18 (CK- 18) and albumin (ALB), were detected in THL cells. The results of CCK- 8 indicated that a medium containing 15 mM glucose showed optimal cell viability of THL. Conversely, elevating glucose concentrations beyond 50 mM markedly impaired THL cell viability. Western blot and qRT-PCR were employed to assess the gene and protein expression in cells treated with varying concentrations of glucose. The results of cells incubated with 0 mM, 15 mM, and 50 mM glucose concentrations showed that compared with the 0 mM glucose group, 15 mM glucose could increase the gene expression of glucokinase (<i>gk</i>) and decrease the gene expression of cytosolic phosphoenolpyruvate carboxykinase (<i>cpepck</i>), mitochondrial phosphoenolpyruvate (<i>mpepck</i>), glucose- 6-phosphatase 1 (<i>g6pase1</i>), forkhead box o1 (<i>foxo1</i>), and glucose-regulated protein 78 (<i>grp78</i>). Compared to 15 mM glucose treatment, the expression of <i>gk</i> in the 50 mM group was significantly decreased, but the expression of <i>cpepck</i>, <i>mpepck</i>, <i>g6pase1</i>, <i>foxo1</i>, and <i>grp78</i> was significantly increased. Moreover, the protein expression of FoxO1 and GRP78 in 50 mM treatment group was significantly increased compared to that in the15 mM group. In the present study, it was found that excessive glucose level can activate the pathways of FoxO1-mediated gluconeogenesis and GRP78-mediated endoplasmic reticulum stress and reduce the glycolytic pathway, thus disrupting the glucose homeostasis in hepatocytes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological and miRNA-mRNA Analyses Provide New Insights into the Immune Response of Clams to Vibrio Infection","authors":"Jianxin Jia,&nbsp;Hongtao Nie","doi":"10.1007/s10126-025-10454-9","DOIUrl":"10.1007/s10126-025-10454-9","url":null,"abstract":"<div><p>Manila clam plays a crucial role in China’s marine aquaculture industry. However, frequent vibriosis outbreaks severely hinder sustainable and healthy development of the shellfish aquaculture industry. This study indicated markedly decreased clam survival rates after 48 h of <i>Vibrio alginolyticus</i> challenge. Gill and hepatopancreas damage was investigated through histological observation. The activity of lysozyme in the gills and hepatopancreas peaked at 12 and 24 h, respectively. <i>V. alginolyticus</i> showed a maximum bacterial load in the gills and hepatopancreas at 12 and 24 h, respectively. Additionally, transcriptome sequencing of hepatopancreas revealed ten differentially expressed miRNAs in Va and Cn after 48 h infection with <i>V. alginolyticus</i>, corresponding to 100 target genes, with eight upregulated and two downregulated DE miRNAs. Gene ontology (GO) enrichment analysis identified 50 known miRNAs and 111 novel miRNAs, thereby predicting a total of 1840 target genes. KEGG analysis revealed significant changes in multiple signaling pathways, involving lysosomes, apoptosis, amino acid metabolism, and endocytosis, in response to <i>V. alginolyticus</i> stimulation. This study provided new information regarding the immune regulation mechanisms of <i>R. philippinarum</i> in response to <i>V. alginolyticus</i> stress.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Conchocelis of Red Algae Bangia sp. in the Nacreous Layer of Akoya Pearl Oyster Pinctada fucata Shell","authors":"Yukino Mizutani,&nbsp;Naohisa Kato,&nbsp;Misato Okamura,&nbsp;Kiyohito Nagai,&nbsp;Kaoru Maeyama,&nbsp;Michio Suzuki,&nbsp;Toshiyuki Shibata,&nbsp;Makoto Kakinuma,&nbsp;Daisuke Funabara","doi":"10.1007/s10126-025-10455-8","DOIUrl":"10.1007/s10126-025-10455-8","url":null,"abstract":"<div><p>We found filamentous structures resembling conchocelis of red algae within the nacreous layer of the Akoya pearl oyster <i>Pinctada fucata</i> shell. Microscopic analyses revealed that these filamentous structures had an autofluorescence and varied in length, occasionally branched, and exhibited diameters ranging from 2 to 5 μm—consistent with previously reported sizes for Bangiales conchocelis. We extracted DNA from organic matrix sheets obtained by decalcifying the shell and performed PCR with primers targeting red algae from the family Bangiales to identify these structures. Sequencing analysis confirmed a 94% match with that of <i>Bangia fuscopurpurea</i>, establishing the presence of <i>Bangia</i> conchocelis within the shell. Conchocelis distributed predominantly near the anterior ear across all examined shells. In 3-year-old oysters, conchocelis filament densities reached approximately 250 filaments per 1 mm², with a broader distribution area than a 2-year-old oyster, where densities were lower, peaking at 35 filaments per 1 mm². Conchocelis distribution was consistent between the left and right shells of the identical oyster. These findings suggest that conchocelis density and distribution expand with oyster growth, providing insights into the age-related dynamics of conchocelis localization in Akoya pearl oyster shells.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10455-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages","authors":"Maria Papadaki,&nbsp;Ngoc-Son Le,&nbsp;Constantinos C. Mylonas,&nbsp;Elena Sarropoulou","doi":"10.1007/s10126-025-10444-x","DOIUrl":"10.1007/s10126-025-10444-x","url":null,"abstract":"<div><p>Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three <i>fanc</i> genes (<i>fancc</i><i>, </i><i>fancl,</i> and <i>fancd2</i>), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream (<i>Sparus aurata</i>), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of <i>fancl</i> and <i>fancd2</i> in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of <i>fancd2</i>, <i>fancc</i> and <i>fancl</i>, respectively. Overall, the data indicated the potential use of <i>fancl</i> and <i>fancd2</i> genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of <i>fanc</i> genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10444-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-(1 → 3) Linked Sulfated Polygalactan from a Seaweed-associated Bacillus velezensis MTCC 13097: A Potential Lead Against Human Hepatocellular Adenocarcinoma","authors":"Sumayya Asharaf,&nbsp;Kajal Chakraborty,&nbsp;Silpa Kunnappilly Paulose,&nbsp;Chesvin Varghese,&nbsp;Shubhajit Dhara","doi":"10.1007/s10126-025-10447-8","DOIUrl":"10.1007/s10126-025-10447-8","url":null,"abstract":"<div><p>Among tumors, liver cancer has an inferior prognosis. Therefore, exploring alternative strategies to improve the effectiveness of treatment for this ailment is of utmost urgency. In this study, we focused on analyzing the anti-cancer properties of bacterial exopolysaccharide from <i>Bacillus velezensis</i> associated with the seaweed <i>Sargassum wightii</i> against hepatocellular adenocarcinoma. A culture-dependent method was used to isolate heterotrophic <i>B. velezensis,</i> which was then evaluated for its antioxidant and anti-cancer properties. A <i>β</i>-(1 → 3) linked sulfated polygalactan exopolysaccharide (BVEP-2) was isolated from the bacterial extract and characterized by spectroscopic analysis. The anti-cancer property was analyzed through assays involving 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red uptake (NRU), and apoptosis (by annexin V-FITC/PI staining) against the human hepatocellular adenocarcinoma cell line (HepG2). BVEP-2 demonstrated potential cytotoxicity in the MTT assay (IC₅₀ 65.05 μg/mL) and 23.02% cell viability in the NRU assay at a 100 µg/mL concentration of BVEP-2 against HepG2, compared to the standard doxorubicin. Potential antioxidant properties of BVEP-2 (IC₅₀ 112–117 µg/mL) corroborated the anti-cancer activities, and the attenuation of free radicals could play a significant role in its anti-cancer potential. BVEP-2 induced approximately 9% early apoptosis and 39% late apoptosis in the HepG2 cell line, whereas the standard drug resulted in around 38% early apoptosis and 37% late apoptosis, along with 6% necrotic cells. The <i>β</i>-(1 → 3) linked sulfated polygalactan exopolysaccharide (BVEP-2) of <i>B. velezensis</i> MTCC13097 showed potential antioxidant and anti-cancer activities, and thus, could be developed as a promising pharmacophore lead against human hepatocellular adenocarcinoma.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Compounds Derived from Marine Halophilic Bacteria: Studies on In Vitro and In Silico Docking","authors":"Arul Dhayalan,&nbsp;Balasubramani Govindasamy,&nbsp;Ashwini Ramesh,&nbsp;Suvithra Sakkarabani,&nbsp;Abdulwahed Fahad Alrefaei,&nbsp;Muthu Thiruvengadam,&nbsp;Deepak Paramasivam","doi":"10.1007/s10126-025-10450-z","DOIUrl":"10.1007/s10126-025-10450-z","url":null,"abstract":"<div><p>Halophilic marine bacteria have been considered for their potential secondary metabolites that possess antagonistic activity, bio-reducing activity, and a number of clinical and probiotic applications. In total, 12 halophilic bacterial strains were isolated from marine water and soil sediment samples. From the primary antimicrobial screening of the cell-free supernatant of all isolates, T7 and N5 strains showed significant results. The genomic DNA of these strains was isolated and their nucleotide sequencing confirmed as <i>Vibrio alginolyticus</i> (T7) and <i>Aeromonas veronii</i> (N5), respectively. The bacterial secondary metabolites from ethyl acetate extract (EAE) were tested against pathogens (<i>Vibrio harveyi</i>, <i>Vibrio parahaemolyticus</i>, <i>Vibrio</i> spp., and <i>Aeromonas hydrophila</i>). The Fourier transform infrared (FT-IR) characterization of the EAE revealed nine functional groups, primarily alkanes and amides, in each isolate (T7 and N5), while gas chromatography mass spectrometry (GC–MS) identified nine compounds from <i>V. alginolyticus</i> (T7) and four from <i>A. veronii</i> (N5). The molecular docking was performed for 12 compounds against proteins from pathogens: DNA gyrase subunit B and the topoisomerase IV (Topo IV), which revealed the potential binding mode of the ligands to the sites of the appropriate targets. Whereas the compound decyl phthalate from <i>V. alginolyticus</i> showed an active binding affinity value − 5.5 to − 6.6 kcal/mol against gyrB and Topo IV proteins of pathogens, compounds phthalic acid, 2-ethylhexyl pentadecyl ester, and bis(2-ethylhexyl) phthalate from <i>A. veronii</i> showed high binding affinity values of − 6.1 to − 7.2 and − 5.7 to − 6.8 kcal/mol against fish pathogen proteins of the gyrB protein and Topo IV, respectively. Thus, the protein with ligand binding affinity indicates a stronger inhibition of <i>V. harveyi</i> and <i>A. hydrophila</i> compared to other pathogens. Overall, the compounds from the EAE of <i>A. veronii</i> were found to be highly effective against pathogens compared to the EAE of <i>V. alginolyticus</i>. Therefore, further studies are needed to explore the potential therapeutic applications in treating bacterial infections in aquaculture settings.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpenoids with Antiangiogenic Activity from Formosan Soft Coral Caementabunda hypotentaculata","authors":"Hsin Huang,&nbsp;Chen-Yu Yang,&nbsp;Shih-Wei Wang,&nbsp;Limbadri Salendra,&nbsp;Shu-Rong Chen,&nbsp;Tzi-Yi Ke,&nbsp;Yu-Liang Yang,&nbsp;Yu-Chi Lin,&nbsp;Yuan-Bin Cheng","doi":"10.1007/s10126-025-10452-x","DOIUrl":"10.1007/s10126-025-10452-x","url":null,"abstract":"<div><p>Soft corals of the genus <i>Caementabunda</i> (synonym <i>Cespitularia</i>) were reported to possess terpenoids with various carbon skeletons. In this research, samples of <i>C. hypotentaculata</i> were collected before and after spawning (in May and August) to compare their secondary metabolites. With the aid of LC–MS/MS molecule networking, a series of unknown verticillane-type diterpenoids were targeted. After repeated column chromatography, five new verticillane-type diterpenoids (<b>1</b>‒<b>5</b>) and one new eudesmanolide-type sesquiterpenoid (<b>6</b>), together with twenty-four known compounds (<b>7</b>‒<b>30</b>), were identified from the Formosan soft coral <i>C. hypotentaculata</i>. Their structures were determined by UV, IR, MS, and NMR analyses, together with the biogenetic considerations of terpenoids. Our pharmacological study showed that the ethanol extract of <i>C. hypotentaculata</i> exhibited antiangiogenic potential against human endothelial progenitor cells. Among all isolated compounds, compound <b>30</b> demonstrated a moderate antiangiogenic effect with an IC₅₀ value of 29.0 ± 0.6 μM.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}