Marine Biotechnology最新文献_第2页

Correction to: HcN13, a Fibroblast Growth Factor Binding Protein, Is Associated with Shell Formation in Hyriopsis cumingii 更正：成纤维细胞生长因子结合蛋白HcN13与三角帆蚌的壳形成有关。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-24 DOI: 10.1007/s10126-025-10520-2

Can Jin, Xiaoyang Tan, Jiayi Zhang, Fangmengjie Wei, Wen Luo

引用次数: 0

Comparisons of Methods for Mucus Sampling and Mucin Semi-Quantification on Barramundi (Lates calcarifer) and Atlantic Salmon (Salmo salar) Epithelial Sites Barramundi （Lates calcarifer）和Atlantic Salmon （Salmo salar）上皮部位粘液取样和黏液半定量方法的比较。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-24 DOI: 10.1007/s10126-025-10515-z

Kyung Min Lee, John Benktander, James W. Wynne, Joel Slinger, Richard S. Taylor, Sara K. Lindén

{"title":"Comparisons of Methods for Mucus Sampling and Mucin Semi-Quantification on Barramundi (Lates calcarifer) and Atlantic Salmon (Salmo salar) Epithelial Sites","authors":"Kyung Min Lee, John Benktander, James W. Wynne, Joel Slinger, Richard S. Taylor, Sara K. Lindén","doi":"10.1007/s10126-025-10515-z","DOIUrl":"10.1007/s10126-025-10515-z","url":null,"abstract":"<div>Fish epithelial surfaces are covered by a mucus layer. The highly glycosylated proteins called mucins are a main component of the mucus, which also contains a range of antibacterial enzymes, proteins, and peptides of importance for its protective properties. Here, we compared the practicality and yield of mucus harvesting from barramundi and Atlantic salmon epithelial sites using glass slide, swab, Super·SAL™ and whole tissue extract. We also compared the feasibility of using the orcinol assay, a glycan-on-membrane assay, and absorbance at 230 nm in combination with standard curves of pig gastric mucin to estimate the mucin concentration. Glycomics demonstrated that non-amine hexose content differed more between fish and tissues than terminal monosaccharides with cis-hydroxy groups, and that non-mucin molecules had a major impact on the A230-based results, making the glycan-on-membrane assay the most versatile method for estimating mucin concentration. We conclude that the most versatile tool for mucus harvesting was swabs, allowing for sufficient amounts of sample to be harvested with relative ease and low levels of contamination from the oral cavity, gill, skin, and intestine. Furthermore, the glycan-on-membrane assay was useful for measuring mucus concentration, and it was beneficial to estimate both sample concentration and purity by comparing samples at relatively similar concentrations.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10515-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Probiotic Intervention Reshapes Host Microbiota and Metabolite Profiles to Enhance Disease Resistance in Micropterus salmoides 功能性益生菌干预重塑寄主微生物群和代谢物谱以增强沙尾小翼的抗病能力

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-12 DOI: 10.1007/s10126-025-10514-0

Huijuan Wang, Ling Lin, Wangyang Jin, Zibin Liang, Siling Hu, Aiyi Zhu, Lihua Jiang

{"title":"Functional Probiotic Intervention Reshapes Host Microbiota and Metabolite Profiles to Enhance Disease Resistance in Micropterus salmoides","authors":"Huijuan Wang, Ling Lin, Wangyang Jin, Zibin Liang, Siling Hu, Aiyi Zhu, Lihua Jiang","doi":"10.1007/s10126-025-10514-0","DOIUrl":"10.1007/s10126-025-10514-0","url":null,"abstract":"<div>In this study, Lactobacillus plantarum, Bacillus subtilis, and their 1:1 mixture were introduced into the aquaculture system of Micropterus salmoides for a duration of 42 days. Subsequently, a challenge test with Aeromonas hydrophila was performed. The objective was to evaluate the immunomodulatory and microbiota-regulating effects of these probiotics, with emphasis on intestinal microbial composition and serum metabolome shifts in treated versus control groups before and after infection. Results revealed a significant reduction in the relative abundance of A. hydrophila in the intestines of probiotic-treated fish (p < 0.05). Histological observations indicated improved intestinal integrity in treated groups. Metabolomic analysis identified significant alterations in serum compounds, particularly in carboxylic acids, organic oxygen compounds, glycerophospholipids, and fatty acyl derivatives (p < 0.05). Post-challenge, levels of pro-inflammatory metabolites were significantly lower in probiotic groups compared to controls. Microbial network analysis showed increased community modularity and stability in treated fish, with key shifts toward dominant genera such as Plesiomonas. These findings demonstrate that L. plantarum and B. subtilis effectively modulate host gut microbiota, reshape systemic metabolite profiles, and enhance immune resilience against bacterial infection. The observed microbiota–metabolome interactions underline the potential of these probiotics as functional feed additives for promoting fish health. The study provides mechanistic insights supporting the broader application of probiotic interventions in aquaculture and functional food development.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptomic Analysis Reveals Key Growth-Related Genes and Alternative Splicing Events in Siniperca scherzeri 比较转录组学分析揭示了中华鳜生长相关关键基因和选择性剪接事件。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-11 DOI: 10.1007/s10126-025-10516-y

Hao Xu, Jian Jin, Yang Li, Yannian Wu, Zhiqiang Cheng, Maoyuan Wang, Xiaojun Ye, Mingyong Lai, Dongling Zhang

{"title":"Comparative Transcriptomic Analysis Reveals Key Growth-Related Genes and Alternative Splicing Events in Siniperca scherzeri","authors":"Hao Xu, Jian Jin, Yang Li, Yannian Wu, Zhiqiang Cheng, Maoyuan Wang, Xiaojun Ye, Mingyong Lai, Dongling Zhang","doi":"10.1007/s10126-025-10516-y","DOIUrl":"10.1007/s10126-025-10516-y","url":null,"abstract":"<div>Siniperca scherzeri is an important aquaculture species in China with good disease resistance, but its growth rate is relatively slow. To uncover the molecular mechanisms underlying growth trait differences, this study employed RNA-seq technology to analyze the liver tissues of fast-growing (FG) and slow-growing (SG) individuals of S. scherzeri. A total of 875 differentially expressed genes (DEGs) and 622 differentially alternatively spliced genes (DSGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the DEGs were mainly involved in skeletal development, extracellular matrix remodeling, signal transduction, and cell growth and apoptosis, whereas the DSGs were significantly enriched in processes such as RNA splicing and processing, protein degradation, ribosome biogenesis, muscle structure and contraction, and were associated with multiple energy metabolism and signaling pathways. Alternative splicing analysis revealed that, compared with the slow-growth group, the fast-growth group exhibited a higher abundance of alternative splicing events. These results suggest that the growth differences in S. scherzeri may stem from the coordinated regulation at both the transcriptional and splicing levels, thereby contributing to enhanced growth rates. These findings provide important insights into the molecular basis underlying growth differences in S. scherzeri and offer valuable resources for the identification of potential molecular markers and key functional genes for aquaculture breeding.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a Core Germplasm and Identification of Candidate SNPs Associated with Growth Performance of Epinephelus tukula by Whole-Genome Resequencing 斑点石斑鱼核心种质的构建及生长性能相关候选snp的全基因组重测序

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-10 DOI: 10.1007/s10126-025-10502-4

Liu Cao, Jun Ma, Ningyiming Hong, Jiaoli Yao, Yan Lu, Yatong Wu, Lei Tang, Pan Chen, Xingrong Hou, Ning Yang, Hai Huang

{"title":"Construction of a Core Germplasm and Identification of Candidate SNPs Associated with Growth Performance of Epinephelus tukula by Whole-Genome Resequencing","authors":"Liu Cao, Jun Ma, Ningyiming Hong, Jiaoli Yao, Yan Lu, Yatong Wu, Lei Tang, Pan Chen, Xingrong Hou, Ning Yang, Hai Huang","doi":"10.1007/s10126-025-10502-4","DOIUrl":"10.1007/s10126-025-10502-4","url":null,"abstract":"<div>Epinephelus tukula is an economically important aquaculture animal, and a major parent in grouper crossbreeding. To better preserve and exploit E. tukula germplasm resources, a core collection (containing 34 individuals derived from 10 genetic groups) was first constructed based on phenotypic growth traits and whole-genome resequencing (WGS) data. The phenotypic traits of the individuals within the core collection were not significantly different from those in the original collection, suggesting effective representativeness of the core collection. Additionally, we performed genome-wide association study (GWAS) of E. tukula to identify candidate single nucleotide polymorphisms (SNPs) and genes associated with growth traits, to facilitate the improvements in the growth performance of this species. Twenty-six significant SNPs were identified, scattered among multiple chromosomes. Five SNPs were confirmed to be correlated with growth in another new group of 101 individuals. Based on the annotation results, these five SNPs were located in CCDC102A, NTRK2, CTSL, OTOF, and nestin, and were involved in cell development, differentiation and proliferation, glycolytic metabolism, neurological development, and myoblast differentiation. Our findings not only provide an effective basis for the conservation and utilization of E. tukula germplasm resources, but also promote the development of marker-assisted selection of E. tukula.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10502-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145021650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin B12 is Essential for Growth, Innate Immunity, Antioxidant Capacity, Fatty Acid and Amino Acid Metabolism, and Ammonia Tolerance in Pacific White Shrimp, Penaeus vannamei 维生素B12是凡纳滨对虾生长、先天免疫、抗氧化能力、脂肪酸和氨基酸代谢以及氨耐受性所必需的

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-08 DOI: 10.1007/s10126-025-10512-2

Nalin Medagoda, Kyeong-Jun Lee

{"title":"Vitamin B12 is Essential for Growth, Innate Immunity, Antioxidant Capacity, Fatty Acid and Amino Acid Metabolism, and Ammonia Tolerance in Pacific White Shrimp, Penaeus vannamei","authors":"Nalin Medagoda, Kyeong-Jun Lee","doi":"10.1007/s10126-025-10512-2","DOIUrl":"10.1007/s10126-025-10512-2","url":null,"abstract":"<div>This study assessed the optimum dietary vitamin B12 requirement of Pacific white shrimp, Penaeus vannamei, for growth, feed efficiency, hemocyte counts, innate immunity, and ammonia stress resistance. Semi-purified experimental diets were prepared by adding vitamin B12 at 0.0, 0.5, 1.0, 2.0, and 4.0 mg/kg (Con, CB0.5, CB1.0, CB2.0, and CB4.0) and a negative control (NCon) diet was prepared with 0.3% tetracycline hydrochloride. Four replicate groups of shrimp (0.17 ± 0.01 g, 32 shrimp per tank) were fed one of the diets at 3–6% of body Weight for 8 weeks. CB2.0 diet significantly increased weight gain and feed efficiency of the shrimp compared to Con diet. The expressions of insulin-like growth factor (IGF)-1, IGF-binding protein, regulatory-associated protein of mammalian target of rapamycin-like, crustin, methylmalonyl-CoA mutase, and methionine synthase-like genes in hepatopancreas were significantly upregulated with increasing dietary vitamin B12 levels. Total hemocyte count, nitroblue tetrazolium, lysozyme, and antiprotease activities were significantly increased at higher vitamin B12 inclusion levels above 1.00 mg/kg. Phenoloxidase, superoxide dismutase, glutathione peroxidase activities, and survival during the ammonia challenge were significantly higher in C2.0 group than in Con group. After the challenge test, C4.0 and C1.0 groups exhibited significantly higher nitroblue tetrazolium and phenoloxidase activities, respectively. Increasing dietary vitamin B12 levels linearly increased hemolymph, hepatopancreas, and fecal vitamin B12 levels. The polynomial regression analysis of final body weight indicated that the optimum dietary vitamin B12 requirement for juvenile Pacific white shrimp is 2.02 mg/kg.\u0000</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Onnamides and a Novel Analogue, Onnamide G, as Potent Leishmanicidal Agents Onnamide和一种新的类似物Onnamide G作为有效的利什曼尼杀灭剂

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-05 DOI: 10.1007/s10126-025-10494-1

Takahiro Jomori, Nanami Higa, Trianda Ayuning Tyas, Natsuki Matsuura, Yudai Ueda, Ayumi Suetake, Shin Miyazaki, Shuichi Watanabe, Sei Arizono, Yasuhiro Hayashi, Ko Yasumoto, Yuji Ise, Toshiyuki Wakimoto, Mina Yasumoto-Hirose, Junichi Tanaka, Kanami Mori-Yasumoto

{"title":"Onnamides and a Novel Analogue, Onnamide G, as Potent Leishmanicidal Agents","authors":"Takahiro Jomori, Nanami Higa, Trianda Ayuning Tyas, Natsuki Matsuura, Yudai Ueda, Ayumi Suetake, Shin Miyazaki, Shuichi Watanabe, Sei Arizono, Yasuhiro Hayashi, Ko Yasumoto, Yuji Ise, Toshiyuki Wakimoto, Mina Yasumoto-Hirose, Junichi Tanaka, Kanami Mori-Yasumoto","doi":"10.1007/s10126-025-10494-1","DOIUrl":"10.1007/s10126-025-10494-1","url":null,"abstract":"<div>Leishmaniasis is a neglected tropical disease posing significant global health challenges. Given the limited effective treatment options and associated constraints, many patients are unable to complete therapy. In this study, we report the remarkable leishmanicidal activity of onnamides derived from the marine sponge Theonella sp. against the promastigote form of Leishmania major (IC50 = 0.2–140 nM) and demonstrate their selectivity through cytotoxicity assessments on human hepatoma HepG2 cells. Structural-activity relationship (SAR) analyses were conducted using 6,7-dihydro-onnamide A and its analogs. Furthermore, we compared the action mechanisms of amphotericin B, the primary drug currently used for treatment, and the obtained onnamides. Based on biological evaluations and SAR studies, onnamides can be considered promising candidates for anti-leishmanial chemotherapy, warranting further investigation.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10494-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144990550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Reveals Molecular Characteristics and Immune Response in Triploid Cyprinid Fish Infected with a Novel Pathogenic Aeromonas sp. AS1-4 转录组揭示了感染新型致病气单胞菌AS1-4的三倍体鲤科鱼的分子特征和免疫应答

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-05 DOI: 10.1007/s10126-025-10513-1

Qin-Yang He, Jie Peng, Ruo-Xing Yu, Ting Luo, Yao-Hui Li, Zi-Rou Zhong, Zi-Le Qin, Fei Wang, Jian Li, Sheng-Wei Luo

{"title":"Transcriptome Reveals Molecular Characteristics and Immune Response in Triploid Cyprinid Fish Infected with a Novel Pathogenic Aeromonas sp. AS1-4","authors":"Qin-Yang He, Jie Peng, Ruo-Xing Yu, Ting Luo, Yao-Hui Li, Zi-Rou Zhong, Zi-Le Qin, Fei Wang, Jian Li, Sheng-Wei Luo","doi":"10.1007/s10126-025-10513-1","DOIUrl":"10.1007/s10126-025-10513-1","url":null,"abstract":"<div>Triploid cyprinid fish (TCF, 3N = 150) is a novel hybrid fish showing great disease resistance during aquaculture processes. However, the majority of Aeromonas strains act as opportunistic pathogens that can cause a variety of diseases and pose a notable health risk. In this investigation, a novel Aeromonas sp. AS1-4 was retrieved from the spleen (SPL) of moribund cyprinid fish. Whole genome sequencing (WGS) was employed to characterize its virulence features and signal regulation. Thereafter, TCFs were infected with strain AS1-4 for pathological analysis. Strain AS1-4 challenge could cause abnormal glycogen deposition in the liver (LIV) of TCFs, accompanied by significant alteration of physiological response. Furthermore, expression patterns of pivotal immune-related genes were markedly elevated in TCFs post-infection. Transcriptomic analysis revealed that strain AS1-4 infection may exert a profound impact on insulin signaling pathway and steroid biosynthesis. These results may contribute to the comprehension of molecular characteristics in fish infected with Aeromonas sp. AS1-4.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144990552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Analysis of Litopenaeus vannamei: Understanding Salinity Adaptation Mechanisms in Freshwater Environments 凡纳滨对虾转录组学分析：了解淡水环境中的盐度适应机制

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-09-05 DOI: 10.1007/s10126-025-10511-3

Xuenan Li, Xi Cheng, Xilin Dai

{"title":"Transcriptomic Analysis of Litopenaeus vannamei: Understanding Salinity Adaptation Mechanisms in Freshwater Environments","authors":"Xuenan Li, Xi Cheng, Xilin Dai","doi":"10.1007/s10126-025-10511-3","DOIUrl":"10.1007/s10126-025-10511-3","url":null,"abstract":"<div>Litopenaeus vannamei exhibits strong salinity adaptation; however, its survival and growth are significantly reduced in freshwater environments. To investigate the response mechanisms of L. vannamei to freshwater conditions, gill tissues from shrimp cultured for 30 days in both freshwater and seawater environments were used as experimental material in this study. Transcriptome sequencing was performed using the Illumina platform to analyze differentially expressed genes (DEGs) and regulatory pathways associated with salinity stress. Additionally, real-time quantitative PCR was employed to validate the transcriptome findings. A total of 1173 DEGs were identified between the groups, including 408 upregulated and 765 downregulated genes. These DEGs were primarily associated with pathways related to ATP binding, metabolic processes, oxidative phosphorylation, purine metabolism, and phagosome formation. Litopenaeus vannamei appears to adopt to salinity stress by enhancing energy metabolism pathways such as oxidative phosphorylation to meet the increased energy demands of osmoregulation. The upregulation of the glutathione metabolism pathway likely contributes to mitigating oxidative damage induced by salinity stress. Furthermore, the enrichment of genes in the structural constituent of cuticle pathway suggests a role in ion regulation and maintenance of osmotic balance. This study provides foundational data for advancing the understanding of physiological response mechanisms in L. vannamei under freshwater aquaculture conditions.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144990551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seas of Renewal: Turning Sea Urchin Waste into Polyhydroxynaphtoquinone-Collagen Biomaterials for Regenerative Medicine 再生之海：将海胆废物转化为再生医学用多羟基萘醌-胶原蛋白生物材料

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2025-08-30 DOI: 10.1007/s10126-025-10504-2

Giordana Martinelli, Stefania Marzorati, Margherita Roncoroni, Luciano Magro, Matteo Brilli, Giangiacomo Beretta, Graziano Colombo, Luca Melotti, Anna Carolo, Giulia Zivelonghi, Stefano Farris, Marco Patruno, Raffaella Soave, Mario Italo Trioni, Michela Sugni

{"title":"Seas of Renewal: Turning Sea Urchin Waste into Polyhydroxynaphtoquinone-Collagen Biomaterials for Regenerative Medicine","authors":"Giordana Martinelli, Stefania Marzorati, Margherita Roncoroni, Luciano Magro, Matteo Brilli, Giangiacomo Beretta, Graziano Colombo, Luca Melotti, Anna Carolo, Giulia Zivelonghi, Stefano Farris, Marco Patruno, Raffaella Soave, Mario Italo Trioni, Michela Sugni","doi":"10.1007/s10126-025-10504-2","DOIUrl":"10.1007/s10126-025-10504-2","url":null,"abstract":"<div>Chronic wounds and skin ulcers pose significant challenges to healthcare systems globally, necessitating innovative approaches to accelerate healing processes. Biomaterial-based therapies have emerged as promising solutions for tissue regeneration. This study focuses on valorization of sea urchin waste toward the development and characterization of collagen-based scaffolds added with polyhydroxynaphthoquinone (PHNQ) antioxidants, successfully incorporated into biomaterials at optimal ratio, enhancing scaffold stability and integrity. Water uptake, mechanical properties, and degradation kinetics of the composite scaffolds were evaluated and compared with controls. Biocomposites were also tested for cytotoxicity. Results indicate that composite scaffolds exhibit superior chemical stability and slower degradation rates, attributed to strong interactions between collagen and PHNQs. This aspect was explored also through in silico investigations by means of tight binding molecular dynamics methods. It has been found that a covalent bond forms between the selected collagen representative and one PHNQ. Furthermore, the antioxidant activity of PHNQs was retained in the composite scaffolds, providing additional therapeutic benefits under the perspective application of regenerative medicine. Normal human dermal fibroblasts (NHDF) exposed to the combination of collagen and PHNQs remained viable. Overall, these findings highlight the potential of sea urchin food waste in a valorization chain, offering added value through the production of collagen-based composite scaffolds.\u0000</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10504-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0