Marine Biotechnology最新文献

{"title":"Gonadal Lipid Storage in Mytilus coruscus: A Comprehensive Gene Network and Key Gene Discovery.","authors":"Zhenqi Xin, Hao Wang, Bingqi Wei, Pengzhi Qi, Xiaojun Yan, Zhi Liao, Baoying Guo, Weifeng Wang","doi":"10.1007/s10126-025-10475-4","DOIUrl":"https://doi.org/10.1007/s10126-025-10475-4","url":null,"abstract":"<p><p>The hard-shelled mussel (Mytilus coruscus), a commercially vital bivalve in China, accumulates lipids predominantly in its gonads, the species' primary edible tissue. Understanding the molecular mechanisms underlying gonad-specific lipid storage is critical for improving reproductive efficiency and aquaculture yield. This study employs comparative transcriptomic analysis of multiple tissues (gonad, gill, mantle, foot, hemolymph) to pinpoint key regulatory genes involved in lipid deposition. Through weighted gene co-expression network analysis (WGCNA), vitellogenin (VG), perilipin (PLIN), and transmembrane protein (TM) were identified as hub genes in gonadal lipid regulation. Genomic characterization revealed 13 VG and three PLIN family members in M. coruscus, which displayed conserved structural motifs and were syntenic with related bivalves, underscoring their functional significance. Phylogenetic analyses further highlighted the evolutionary conservation of these lipid-associated genes across marine invertebrates. Concurrently, sex-specific metabolic divergence was investigated. Physiological validation demonstrated that ovarian crude fat content exceeded testicular levels by 36%, corroborated histologically by larger, more stable lipid droplets in female gonads. Sex-specific expression profiling uncovered pronounced divergence: VG and PLIN were markedly enriched in ovaries, whereas glucose-6-phosphatase (G6P), a driver of energy catabolism, was elevated in testes. This study provides a molecular framework for understanding reproductive lipid metabolism in bivalves, offering biomarkers to refine broodstock management and aquaculture practices.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":"99"},"PeriodicalIF":2.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"eDNA-Based Evaluation of the Haplotypic Diversity of Orange-Spotted Grouper (Epinephelus coioides) in Stock Enhancement Areas of Wanshan Archipelago.","authors":"Chun Hui Ai, Yi Long Lin, Ming Ze Chen, Jun Hong Xia","doi":"10.1007/s10126-025-10471-8","DOIUrl":"10.1007/s10126-025-10471-8","url":null,"abstract":"<p><p>The orange-spotted grouper (Epinephelus coioides) is an economically important marine species in the South China Sea. Due to overfishing and environmental pollution, its natural resources in the South China Sea have severely declined. Evaluation of genetic diversity of the orange-spotted grouper in the distribution areas is important for future conservation action. In this study, a molecular marker specific for orange-spotted grouper E. coioides was developed based on the mitochondrial D-loop region sequences. We evaluated the community structure and genetic diversity of the groupers at four stock enhancement sites of the Wanshan Archipelago by employing integrated assessment methods including environmental DNA (eDNA) and the D-loop marker. Five grouper species were identified from the eDNA samples using 12S rDNA metabarcoding technology, with the orange-spotted grouper being the most abundant grouper ranging from 39.05% to 49.79% of the grouper contents. Furthermore, 15 D-loop haplotypes for the orange-spotted grouper in the Wanshan Archipelago release areas were detected by utilizing the novel developed D-loop marker. High haplotype genetic diversity was observed at all sampling sites for the orange-spotted grouper population. Dominant haplotypes such as ASV_1, ASV_2, ASV_3, ASV_4, and ASV_5 exhibited high levels of geographic sharing, suggesting a degree of ecological or environmental similarity across these regions. Most of the genetic variations were originated within populations, indicating significant genetic differentiation among grouper populations in the Wanshan Archipelago. Our study indicates that eDNA technology is a valuable non-invasive tool for monitoring fish community structure, and the haplotype diversity of E. coioides in the sampled waters maintained a relatively high haplotype richness.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":"98"},"PeriodicalIF":2.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Temporal Dynamics of Gonadal Development and Sexually Dimorphic Gene Expression During Sex Differentiation in Mandarin Fish (Siniperca chuatsi).","authors":"Yi Rong, Yuanyuan Wang, Jinzhi Zhao, Lei Chen, Kuangxin Chen, Binbin Tao, Yanlong Song, Jianxiong Yin, Kewei Zeng, Wen Song, Wei Hu, Ji Chen","doi":"10.1007/s10126-025-10478-1","DOIUrl":"https://doi.org/10.1007/s10126-025-10478-1","url":null,"abstract":"<p><p>The mandarin fish (Siniperca chuatsi) is a significant freshwater aquaculture species in China, exhibiting sexual dimorphism during growth. However, the cellular and molecular mechanisms underlying early gonadal development and sex differentiation remain unclear. This study traces early gonadal development by examining changes in gonadal histology, germ cell morphologic and distributional changes, and the expression of key signaling molecules. H&E staining and immunofluorescence analysis revealed that oogonia and spermatogonia first appeared at 27 and 33 days post-fertilization (dpf), respectively, marking the onset of sex differentiation. By 51 and 57 dpf, oocytes and spermatocytes became more abundant, indicating the completion of sex differentiation. We examined the expression of female-biased (cyp19a, dmrt2 and foxl2a), male-biased (amh, dmrt1, gsdf and sox9), and meiotic genes (dmc1 and sycp3). In male gonads, the expression of amh was detected at 21-42 dpf, and it showed significantly higher expression after 42 dpf compared to females. Meanwhile, the expression of dmrt1 was significantly higher in male gonads than in female gonads at 30 to 33 dpf. The dmrt3 gene had high expression levels in both male and female gonads before 45 dpf, with a further increase observed after 51 dpf. The gsdf gene exhibited higher expression levels in male gonads between 27 and 42 dpf than at other time points. Additionally, Sox9 expression was significantly higher levels observed in males than in females at 33 dpf. In female gonads, cyp19a was consistently expressed from 21 to 42 dpf and was significantly higher than that in males after 42 dpf, dmrt2 expression was higher than that in males at 36-42 dpf, foxl2a expression was higher at 24-30 dpf and significantly higher than that in males at 36-45 dpf. At 27 dpf, the meiosis-related genes dmc1 and sycp3 were detected, with expression patterns aligning with germ cell development and sex differentiation. In conclusion, by characterizing germ cell morphology and analysing expression changes in sex-related genes, we confirmed that sex determination in mandarin fish occurs before 27 dpf. In females, sex differentiation progresses from 27 to 51 dpf, whereas in males, it occurs from 33 to 57 dpf.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":"97"},"PeriodicalIF":2.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription Dynamics and DNA Methylation Responses to Growth Modification.","authors":"Kris A Christensen, Étienne Collette, Danielle Perley, Dionne Sakhrani, Annette F Muttray, Rosalind A Leggatt, Carlo A Biagi, Ben F Koop, Robert H Devlin","doi":"10.1007/s10126-025-10476-3","DOIUrl":"10.1007/s10126-025-10476-3","url":null,"abstract":"<p><p>To better understand how gene transcription is controlled under different physiological and environmental conditions, we assessed transcription and methylation responses in a vertebrate system (salmon) where growth can be manipulated in response to growth hormone transgenesis or under satiated, feed-deprived, and re-fed experimental conditions. In both transgenic and non-transgenic liver tissue, methylation of gene promoters was negatively associated with transcription at all measured times. However, the changes in promoter methylation among time-points did not generally correlate with changes in gene transcription among treatments. The results from this study suggest that only a subset of genes are readily responsive to changes in promoter methylation for a given environmental shift such as feed-deprivation or a physiological shift such as growth hormone transgenesis (i.e., genotype-by-environment interactions). The present study also reveals the complexity of how one tissue responds to alterations in internal changes in physiology (growth modification by growth hormone transgenesis; genotype), external environmental conditions (food availability; environment), and their interactions (genotype-by-environment effects).</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"96"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of an Extracellular Poly(3-hydroxybutyrate) Depolymerase in the Genus Alteromonas and Its Phylogenetic Distribution Among Alteromonas Species.","authors":"Kyogo Iseki, Shin-Ichi Hachisuka, Hiroshi Kikukawa, Takeharu Tsuge, Ken'ichiro Matsumoto","doi":"10.1007/s10126-025-10477-2","DOIUrl":"10.1007/s10126-025-10477-2","url":null,"abstract":"<p><p>Poly(3-hydroxybutyrate), P(3HB), is an aliphatic polyester that is susceptible to biodegradation even in marine environments. The high biodegradability of P(3HB) can be attributed to the presence in the environment of extracellular P(3HB) depolymerase (PhaZ), the initial enzyme involved in P(3HB) degradation. In this study, we aimed to identify the gene encoding PhaZ in the marine P(3HB)-degrading bacterium Alteromonas sp. D210916BOD_24, which was previously isolated. First, we conducted genome analysis of the strain, revealing that the strain possesses a PhaZ homolog. It possesses a catalytic domain with a lipase box near the center, a substrate-binding domain comprising two regions, and a fibronectin type III linker domain, which fit the marine bacterial domain pattern. Disruption of the PhaZ homolog gene caused the strain to lose its P(3HB) degradation ability. The recombinant PhaZ homolog protein exhibited significant activity toward P(3HB). The results indicated that the PhaZ homolog indeed functions as PhaZ in Alteromonas sp. D210916BOD_24. Furthermore, we focused on the distribution and genomic placement of PhaZ homolog genes. The PhaZ homolog was present in 4/20 Alteromonas strains examined, with Alteromonas sp. D210916BOD_24 showing substantial divergence from the other three strains in the 16S rDNA-based phylogenetic tree. The gene arrangement around the PhaZ homolog gene was clearly different between Alteromonas sp. D210916BOD_24 and the others. Additionally, large-scale gene locations and orientations exhibited considerable differences. These findings suggest that the horizontal transfer of PhaZ homolog genes occurred after a certain degree of species division.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"95"},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple Transgene Overexpression using Scallop Hemocyte Culture Platform Enables Functional Genetic Research in Molluscs.","authors":"Jeongwoong Yoon, Taro Tsuda, Enrico Bortoletto, Akari Sakaguchi, Mutsuko Kobayashi, Umberto Rosani, Hayato Yokoi, Makoto Osada, Paola Venier, Kazue Nagasawa","doi":"10.1007/s10126-025-10472-7","DOIUrl":"10.1007/s10126-025-10472-7","url":null,"abstract":"<p><p>Despite being evolutionarily and commercially important, molluscs have been a traditionally challenging group to study, due to their difficulty in maintenance under lab conditions and the lack of a genetic toolkit. Previously, we showed that transgene expression can be attained in molluscan cells with reporter genes under a molluscan virus promoter sequence. Following up, we developed a simple, efficient and rapid transgene expression platform using primary hemocyte culture of Farrer's scallop Chlamys farreri, a marine bivalve mollusc. The protocol consists of two steps: collection and seeding of hemocytes and incubation for 1 to 4 days with DNA-reagent mixture. We evaluated seven transfection reagents for three bivalve species and found that X-tremeGENE 360 was highly efficient for DNA transfection, particularly for C. farreri. Subsequently, C. farreri hemocyte culture and transfection conditions were examined, such as culture medium, size and form of DNA, and the mixing ratio of DNA and transfection reagent. Using this protocol, we visualized the subcellular localization of four bivalve oncogenes, Cf-Mdm2-like, Cf-c-Myc-like, Cf-Mortalin-like, and Cf-Ras-like, tagged with EGFP. Our hemocyte platform provides an easy entry to study cellular and molecular biology of molluscs and can be readily adapted for advanced methods such as live imaging and DNA-protein interaction assays, making the study of molluscs more accessible to the scientific community.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"94"},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Functional Characterization of Two Distinct Muscle Stem Cell Populations in Golden Pompano Trachinotus ovatus.","authors":"Liying Sun, Na Wang, Sanying Huang, Ziyang Luo, Chenhao Zhang, Jie Qi, Yan He","doi":"10.1007/s10126-025-10470-9","DOIUrl":"10.1007/s10126-025-10470-9","url":null,"abstract":"<p><p>Unlike mammals with determinate growth patterns, large-bodied teleost fish exhibit indeterminate growth. Two distinct muscle stem cell populations have been discovered in teleost fish: muscle satellite cells expressing Pax3 and Pax7, akin to those in mammals, and growth-specific stem cells in the external cell layer (ECL) regulated by Meox1. However, their origins and regulatory mechanisms remain elusive in large teleost fishes. In this study, we identified these two stem cell populations in the golden pompano (Trachinotus ovatus), an economically significant teleost species. In situ hybridization revealed that muscle satellite cells are localized in mononucleated cells at the edges of muscle fibers, while growth-specific stem cells are distributed within the myosepta. Interestingly, growth-specific stem cells in T. ovatus differ from those in zebrafish in their origin, distribution, and expression patterns, which could be a contributing factor to T. ovatus's ability for indeterminate growth, whereas zebrafish exhibit determinate growth. Investigations into the repair and regeneration of skeletal muscle following injury demonstrated that Meox1 also plays a role in repairing injured skeletal muscle, although its involvement occurs later than that of Pax3a/Pax7a. In conclusion, our findings confirm the presence of two distinct muscle stem cell populations in teleost fish, shedding light on the complexity of muscle growth. This research provides insights into muscle development and regeneration, with potential applications in aquaculture for improving muscle growth in economically important fish species.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"93"},"PeriodicalIF":2.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Microbiome, Transcriptome, and Physiology Analyses Reveal the Response Of Kuruma Shrimp (Penaeus japonicus) to Oxygen Nanobubble Exposure.","authors":"Yi Chen, Rongwei Zhang, Yuntian Zhang, Jiadong Guo, Zhixu Guo, Yuzhe Han, Fuqiang Wang, Xiaoran Zhao, Tongjun Ren","doi":"10.1007/s10126-025-10464-7","DOIUrl":"10.1007/s10126-025-10464-7","url":null,"abstract":"<p><p>Penaeus japonicus is a high-value crustacean species, but its response to oxygen nanobubble (NB-O₂) remains poorly understood. This study evaluated the effects of NB-O₂ exposure on juvenile P. japonicus cultured in two recirculating aquaculture systems (RAS). The control group (LS) was maintained with low-saturation dissolved oxygen (DO) at 7.5 ± 0.5 mg/L, while the experimental group (HS) was exposed to high-saturation DO of 15.0 ± 0.5 mg/L using NB-O₂ technology. The investigation focused on changes in intestinal microbiota, transcriptomic responses, and physiological adaptation. Results demonstrated that NB-O₂ significantly improved growth performance metrics, including specific growth rate (SGR), feed conversion ratio (FCR), molting rate (MR), and survival rate (SR). The intestinal microbiota exhibited an increased abundance of beneficial microorganisms, such as Lactobacillales and Rhodopseudomonas palustris, while maintaining microbial stability. Transcriptome analysis identified key pathways associated with adaptation to hyperoxia, particularly those involved in amino acid metabolism, energy metabolism, and antioxidant capacity. These findings were supported by physiological analysis, which showed changes in the amino acid profile and total free amino acids (T-AA), as well as a balanced interaction between glycogen (Gn), adenosine triphosphatase (ATP), and lactate dehydrogenase (LDH) in energy regulation. Antioxidant enzyme activities were all elevated under hyperoxia, including peroxidase (POD), catalase (CAT), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px). Additionally, the expression of stress-resistance genes (HSP70, CAP3, LYZ-C) indicated no adverse effects. Overall, P. japonicus exhibited strong physiological and molecular adaptations to NB-O₂-induced hyperoxia, highlighting the potential of this technology to enhance aquaculture productivity.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"92"},"PeriodicalIF":2.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Transformation of the Marine Oleaginous Microalga, Marinichlorella sp. NKG400014.","authors":"Ryota Kumakubo, Kento Sagawa, Tsuyoshi Tanaka","doi":"10.1007/s10126-025-10473-6","DOIUrl":"10.1007/s10126-025-10473-6","url":null,"abstract":"<p><p>Microalgae attract significant attention as promising biofuel sources. However, lack of an efficient genetic transformation system hinders their use in advanced genetic engineering applications. We previously identified the marine oleaginous microalgal strain, NKG400014, from our marine microalgal culture collection via Nile red screening. Subsequent phylogenetic analysis revealed that strain NKG400014 belonged to the new genus, Marinichlorella. To date, successful genetic transformation of Marinichlorella species has not been reported. In this study, we aimed to investigate the growth characteristics of NKG400014 to clarify its oleaginous features as a biofuel producer. Then, we established a technique for NKG400014 genetic transformation via electroporation and optimized the key parameters. Two selectable markers (hygromycin phosphotransferase and neomycin phosphotransferase II genes) were used to assess the transformation efficiency under endogenous and exogenous promoters. Endogenous promoters of Marinichlorella sp. were more efficient for transformation than its exogenous promoters. To the best of our knowledge, this study represents the first successful genetic transformation of Marinichlorella sp.. Further development of genetic tools and metabolic engineering approaches can enhance the lipid productivity of this strain, facilitating the economic production of microalgal biofuels.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":"91"},"PeriodicalIF":2.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Major QTL Associated with Body Color on ChrLG3 in Tilapia by GWAS","authors":"Zong Xian Zhu,&nbsp;Yin Yi Yao,&nbsp;Yi Long Lin,&nbsp;Chun Hui Ai,&nbsp;Jun Hong Xia","doi":"10.1007/s10126-025-10468-3","DOIUrl":"10.1007/s10126-025-10468-3","url":null,"abstract":"<div><p>Body color is one of the most important traits in fish affecting species recognition, mate selection, and its economic value. Dissecting the genetic architecture underlying the body color diversity is important in tilapia. In this study, we successfully dissected the genetic architecture affecting black spot variation in two mapping populations of tilapia (a full-sib family: <i>N</i> = 103 &amp; a mixed population: <i>N</i> = 290) using ddRAD-seq-based GWAS technology. A major QTL interval for black spot trait on ChrLG3 (9.8–14.2 Mb) with a peak located at ChrLG3_11207231 was identified. The significant correlations (<i>P</i> &lt; 0.001) between genotypes and color data were validated by applying a novel microsatellite marker (SSR-BC-1). A total of 14 genome-wide significant SNPs near or under the peak of the major QTL were located in exons or introns of <i>nfib</i> (6) and <i>dennd4c</i> (8). Furthermore, qRT-PCR analysis indicated both genes were differentially expressed in the skin and brain of black and red tilapia. Our study laid a foundation for exploring the genetic mechanism of body color variation and carrying out genetic improvement for color quality in tilapia.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}