Marine Biotechnology最新文献_第3页

In Vivo Effects of Biosilica and Spongin-Like Collagen Scaffolds on the Healing Process in Osteoporotic Rats 生物硅胶和海绵样胶原支架对骨质疏松大鼠愈合过程的体内影响

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-17 DOI: 10.1007/s10126-024-10356-2

Matheus de A. Cruz, Karolyne S. J. Sousa, Ingrid R. Avanzi, Amanda de Souza, Cintia C. S. Martignago, Fernanda V. B. Delpupo, Mariana C. Simões, Julia R. Parisi, Livia Assis, Flávia De Oliveira, Renata N. Granito, Eeva-Liisa Laakso, Ana Renno

{"title":"In Vivo Effects of Biosilica and Spongin-Like Collagen Scaffolds on the Healing Process in Osteoporotic Rats","authors":"Matheus de A. Cruz, Karolyne S. J. Sousa, Ingrid R. Avanzi, Amanda de Souza, Cintia C. S. Martignago, Fernanda V. B. Delpupo, Mariana C. Simões, Julia R. Parisi, Livia Assis, Flávia De Oliveira, Renata N. Granito, Eeva-Liisa Laakso, Ana Renno","doi":"10.1007/s10126-024-10356-2","DOIUrl":"10.1007/s10126-024-10356-2","url":null,"abstract":"<div>Due to bioactive properties, introducing spongin-like collagen (SPG) into the biosilica (BS) extracted from marine sponges would present an enhanced biological material for improving osteoporotic fracture healing by increasing bone formation rate. Our aim was to characterize the morphology of the BS/SPG scaffolds by scanning electron microscopy (SEM), the chemical bonds of the material by Fourier transform infrared spectroscopy (FTIR), and evaluating the orthotopic in vivo response of BS/SPG scaffolds in tibial defects of osteoporotic fractures in rats (histology, histomorphometry, and immunohistochemistry) in two experimental periods (15 and 30 days). SEM showed that scaffolds were porous, showing the spicules of BS and fibrous aspect of SPG. FTIR showed characteristic peaks of BS and SPG. For the in vivo studies, after 30 days, BS and BS/SPG showed a higher amount of newly formed bone compared to the first experimental period, observed both in the periphery and in the central region of the bone defect. For histomorphometry, BS/SPG presented higher %BV/TV compared to the other experimental groups. After 15 days, BS presented higher volumes of collagen type I. After 30 days, all groups demonstrated higher volumes of collagen type III compared to volumes at 15 days. After 30 days, BS/SPG presented higher immunostaining of osteoprotegerin compared to the other experimental groups at the same experimental period. The results showed that BS and BS/SPG scaffolds were able to improve bone healing. Future research should focus on the effects of BS/SPG on longer periods in vivo studies.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration and Characterization of Antimicrobial Peptides from Shrimp Litopenaeus Vannamei by A Genomic and Transcriptomic Approach 通过基因组和转录组方法探索和表征凡纳滨对虾的抗菌肽

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-14 DOI: 10.1007/s10126-024-10358-0

Xinxin Shan, Bin Yin, Xuzheng Liao, Bang Xiao, Jianguo He, Chaozheng Li

{"title":"Exploration and Characterization of Antimicrobial Peptides from Shrimp Litopenaeus Vannamei by A Genomic and Transcriptomic Approach","authors":"Xinxin Shan, Bin Yin, Xuzheng Liao, Bang Xiao, Jianguo He, Chaozheng Li","doi":"10.1007/s10126-024-10358-0","DOIUrl":"10.1007/s10126-024-10358-0","url":null,"abstract":"<div>Antimicrobial peptides (AMPs) are crucial in the humoral immunity aspect of invertebrates' innate immune systems. However, studies on AMP discovery in the Pacific white shrimp (Litopenaeus vannamei) using omics data have been limited. Addressing the growing concern of antibiotic resistance in aquaculture, this study focused on the identification and characterization of AMPs in L. vannamei using advanced genomic and transcriptomic techniques. The genome of L. vannamei was performed to predict and identify a total of 754 AMP-derived genes, distributed across most chromosomes and spanning 24 distinct AMP families, and further identified 236 AMP-derived genes at the mRNA level in hemocytes. A subset of 20 chemically synthesized peptides, derived from these genes, exhibited significant antimicrobial activity, with over 85% showing effectiveness against key bacterial strains such as Staphylococcus aureus and Vibrio parahaemolyticus. The expression patterns of these AMPs were also investigated in different shrimp tissues and at various infection stages, revealing dynamic responses to pathogenic challenges. These findings highlight the significant potential of AMPs in L. vannamei as novel, effective alternatives to traditional antibiotics in aquaculture, offering insights into their diverse structural properties and biological functions. Together, this comprehensive characterization of the AMP repertoire in L. vannamei demonstrates the efficacy of using omics data for AMP discovery and lays the groundwork for their potential applications.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of DNA Insertion-specific Markers Based on the Intron Region of Oplegnathus punctatus itih4b for Genetic Sex Identification 基于Oplegnathus punctatus itih4b内含子区的DNA插入特异性标记的开发，用于基因性别鉴定。

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-13 DOI: 10.1007/s10126-024-10359-z

Yuting Ma, Yongshuang Xiao, Zhizhong Xiao, Jun Li

{"title":"Development of DNA Insertion-specific Markers Based on the Intron Region of Oplegnathus punctatus itih4b for Genetic Sex Identification","authors":"Yuting Ma, Yongshuang Xiao, Zhizhong Xiao, Jun Li","doi":"10.1007/s10126-024-10359-z","DOIUrl":"10.1007/s10126-024-10359-z","url":null,"abstract":"<div>Spotted knifejaw (Oplegnathus punctatus) is a significant marine fish species that exhibits pronounced sexual dimorphism, with males generally exhibiting greater weight and growth rates than females. Therefore, the farming of O. punctatus with a high proportion of males is beneficial for improving the quality and efficiency of the O. punctatus aquaculture industry. Furthermore, the development of a rapid technique in sexing O. punctatus fry will facilitate the selection and breeding of superior male varieties of O. punctatus. In this study, genome-wide scanning, comparative genomics, and structural variation analysis methods were employed to identify and extract the homologous region of the inter-alpha-trypsin inhibitor heavy chain 4 (itih4b) gene on the X and Y chromosomes from the complete genome sequence of O. punctatus. This analysis revealed the presence of a large segment of DNA insertion markers on the Y chromosome in the region. Itih4b plays an important role in the mechanisms that regulate inflammatory and immune responses in multicellular organisms. The method described here involved the design of a pair of primers to amplify two bands of 532 bp and 333 bp in males (individuals with DNA insertion variants in the intron of the itih4b gene). In females (individuals without DNA insertion), only one band of 333 bp could be distinguished by agarose gel electrophoresis. This method shortened the time required to accurately characterize intronic DNA insertion variants and genetic sexes in O. punctatus, thereby improving detection efficiency. This study has significant value for the large-scale breeding of O. punctatus all-male seedlings and provides a reference point for the study of intron variation regulation and RNA shearing in the itih4b gene.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Docosahexaenoic Acid and Eicosapentaenoic Acid Biosynthesis in Isochrysis galbana by Bacillus jeotgali jeotgali 芽孢杆菌促进了半枝莲二十二碳六烯酸和二十碳五烯酸的合成。

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-09 DOI: 10.1007/s10126-024-10337-5

Yijun Xu, Minnan Wu, Jiayi Cao, Yingying Wang, Lin Zhang, Xiaojun Yan, Yanrong Li, Jilin Xu

{"title":"Enhancement of Docosahexaenoic Acid and Eicosapentaenoic Acid Biosynthesis in Isochrysis galbana by Bacillus jeotgali","authors":"Yijun Xu, Minnan Wu, Jiayi Cao, Yingying Wang, Lin Zhang, Xiaojun Yan, Yanrong Li, Jilin Xu","doi":"10.1007/s10126-024-10337-5","DOIUrl":"10.1007/s10126-024-10337-5","url":null,"abstract":"<div>Isochrysis galbana is valuable in aquaculture due to its production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, achieving high yields of polyunsaturated fatty acids (PUFAs) presents challenges, leading to exploration of innovative approaches. This study investigated the influence of Bacillus jeotgali on the growth of I. galbana and its fatty acid composition. Co-culturing I. galbana with B. jeotgali significantly increased chlorophyll a content and cell abundance, particularly at higher bacterial population densities (algae-to-bacteria ratio of 1:10). Physiological and biochemical analyses found elevated soluble protein content in microalgae co-cultured with B. jeotgali, accompanied by decreased superoxide dismutase (SOD) activity. Fatty acid composition analysis demonstrated a distinctive profile in co-cultured I. galbana, characterized by increased PUFAs, especially EPA and DHA. Gene expression analysis indicated an upregulation of desaturase genes (d4FAD, d5FAD, d6FAD, and d8FAD) associated with PUFA synthesis pathway in I. galbana during co-culturing with B. jeotgali. This study advances our understanding of bacteria-microalgae interactions and presents a promising strategy for enhancing the production of DHA and EPA.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10337-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and Transcriptomic Analyses Provide Insights into Nitrite Stress Responses of the Swimming Crab Portunus trituberculatus 通过生理学和转录组分析了解三疣梭子蟹的亚硝酸盐应激反应

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-08 DOI: 10.1007/s10126-024-10353-5

Yi Jiang, Xiaochen Liu, Yan Shang, Jitao Li, Baoquan Gao, Yichao Ren, Xianliang Meng

{"title":"Physiological and Transcriptomic Analyses Provide Insights into Nitrite Stress Responses of the Swimming Crab Portunus trituberculatus","authors":"Yi Jiang, Xiaochen Liu, Yan Shang, Jitao Li, Baoquan Gao, Yichao Ren, Xianliang Meng","doi":"10.1007/s10126-024-10353-5","DOIUrl":"10.1007/s10126-024-10353-5","url":null,"abstract":"<div>Nitrite is a common environmental pollutant in intensive aquaculture systems. In this study, physiological and transcriptomic analyses were performed to investigate nitrite stress responses in the swimming crab Portunus trituberculatus, an important aquaculture species in China. The results revealed that nitrite can affect neurotransmitter signaling via the expression of neurotransmitter receptors such as octopamine receptor (OAR) and 5-hydroxytryptamine receptor (5-HTR), and depress ecdysteroid signaling by downregulating ecdysteroid receptor (EcR) as well as its downstream transcription factors in hepatopancreas. In addition, nitrite suppressed the expression of hemocyanins, the oxygen-transporting protein, which at least partly contributed to tissue hypoxia, resulting in a switchover of energy metabolism from aerobic to anaerobic pathway. To meet the energy demand, glycogens and lipids were mobilized and transported to the hemolymph, and the catabolism of amino acids and fatty acids was enhanced to provide energy for hepatopancreas. β-oxidation of fatty acids, the major process by which fatty acids are oxidized to generate energy, seems to occur mainly not in mitochondria but in peroxisomes. Although the cellular protective mechanisms, including antioxidant defense, heat shock response (HSR), unfolded protein response (UPR), and autophagy, were activated, nitrite-induced cellular stress overwhelmed the repairing capacity and caused significant increase in the levels of apoptosis. These results indicated that nitrite stress influences neurotransmitter and endocrine signaling, disturbs energy metabolism, damages cellular components, and induces apoptosis in P. trituberculatus. The findings of this study provide new insights into nitrite stress response in the swimming crab and provide valuable information for aquaculture management of this species.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Profiling of Gene Expression in Atlantic Salmon (Salmo salar) at Early Stage of Development 大西洋鲑（Salmo salar）早期发育阶段基因表达的转录组分析。

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-07 DOI: 10.1007/s10126-024-10354-4

Najib Abdellaoui, Min Sun Kim

{"title":"Transcriptome Profiling of Gene Expression in Atlantic Salmon (Salmo salar) at Early Stage of Development","authors":"Najib Abdellaoui, Min Sun Kim","doi":"10.1007/s10126-024-10354-4","DOIUrl":"10.1007/s10126-024-10354-4","url":null,"abstract":"<div>For Atlantic salmon development, the most critical phase is the early development stage from egg to fry through alevin. However, the studies investigating the early development of Atlantic salmon based on RNA-seq are scarce and focus only on one stage of development. Therefore, using the RNA-seq technology, the assessment of different gene expressions of various early development stages (egg, alevin, and fry) was performed on a global scale. Over 22 GB of clean data was generated from 9 libraries with three replicates for each stage with over 90% mapping efficiency. A total of 5534 genes were differentially expressed, among which 19, 606, and 826 genes were specifically expressed in each stage, respectively. The transcriptome analysis showed that the number of differentially expressed genes (DEGs) increased as the Atlantic salmon progressed in development from egg to fry stage. In addition, gene ontology enrichment demonstrated that egg and alevin stages are characterized by upregulation of genes involved in spinal cord development, neuron projection morphogenesis, axonogenesis, and cytoplasmic translation. At the fry stage, upregulated genes were enriched in the muscle development process (muscle cell development, striated muscle cell differentiation, and muscle tissue development), immune system (defense response and canonical NF-kappaB signal transduction), as well as epidermis development. These results suggest that the early development of Atlantic salmon is characterized by a dynamic shift in gene expression and DEGs between different stages, which provided a solid foundation for the investigation of Atlantic salmon development.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ontogenetic, Sexual, and Monthly Niche Segregation of Sepia esculenta in the Northern East China Sea Revealed by Stable Carbon and Nitrogen Isotopes 稳定碳和氮同位素揭示东海北部鮨科鱼类的个体发育、性别和月度生态位分化

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-05 DOI: 10.1007/s10126-024-10352-6

Zhenfang Zhao, Guanyu Hu, Long Chen, Yingcong Chen, Fang Guo, Zhou Fang

{"title":"Ontogenetic, Sexual, and Monthly Niche Segregation of Sepia esculenta in the Northern East China Sea Revealed by Stable Carbon and Nitrogen Isotopes","authors":"Zhenfang Zhao, Guanyu Hu, Long Chen, Yingcong Chen, Fang Guo, Zhou Fang","doi":"10.1007/s10126-024-10352-6","DOIUrl":"10.1007/s10126-024-10352-6","url":null,"abstract":"<div>Golden cuttlefish play a significant role in the food web of the East and Yellow Seas and are a valuable fishery resource in Chinese coastal waters. Samples of golden cuttlefish were obtained from the northern East China Sea between September 2021 and March 2022, and stable isotope methods were utilized in this study to examine the variations in the forage ecology of golden cuttlefish. Our findings reveal dynamic shifts in carbon and nitrogen stable isotopes (δ13C and δ15N), highlighting intricate foraging strategies tailored to growth and environmental changes. A notable trend emerges: an initial growth-linked rise in δ13C and δ15N enrichment, followed by seasonal fluctuations mirroring seasonal food availability. The ontogenetic niche evolution displays striking habitat shifts and trophic level escalation in small mantle length stages, transitioning to niche overlap and subtle trophic shifts later on. Sex-specific differences emerge, with females occupying higher trophic levels than males in most samples. This comprehensive study underscores the complexity and adaptability of golden cuttlefish feeding ecology, inviting further inquiry into their intricate relationships within the marine ecosystem.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic Insights Reveal Unrecognized Diversity of Entotheonella in Japanese Theonella Sponges 元基因组研究揭示了日本海螺海绵中尚未认识到的 Entotheonella 多样性。

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-08-05 DOI: 10.1007/s10126-024-10350-8

Sota Yamabe, Kazutoshi Yoshitake, Akihiro Ninomiya, Jörn Piel, Haruko Takeyama, Shigeki Matsunaga, Kentaro Takada

{"title":"Metagenomic Insights Reveal Unrecognized Diversity of Entotheonella in Japanese Theonella Sponges","authors":"Sota Yamabe, Kazutoshi Yoshitake, Akihiro Ninomiya, Jörn Piel, Haruko Takeyama, Shigeki Matsunaga, Kentaro Takada","doi":"10.1007/s10126-024-10350-8","DOIUrl":"10.1007/s10126-024-10350-8","url":null,"abstract":"<div>Numerous biologically active natural products have been discovered from marine sponges, particularly from Theonella swinhoei, which is known to be a prolific source of natural products such as polyketides and peptides. Recent studies have revealed that many of these natural products are biosynthesized by Candidatus Entotheonella phylotypes, which are uncultivated symbionts within T. swinhoei. Consequently, Entotheonella is considered an untapped biochemical resource. In this study, we conducted metagenomic analyses to assess the diversity of Entotheonella in two T. swinhoei Y and two T. swinhoei W (Y and W referring to the yellow and white interior of the sponge, respectively), after separating filamentous bacteria using density gradient centrifugation. We obtained five Entotheonella metagenome-assembled genomes (MAGs) from filamentous bacteria-enriched fractions. Notably, one of these MAGs is significantly different from previously reported Entotheonella variants. Additionally, we identified closely related Entotheonella members present across different chemotypes of T. swinhoei. Thus, our metagenomic insights reveal that the diversity of Entotheonella within Theonella sponges is greater than previously recognized.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility 二倍体和三倍体太平洋牡蛎性腺中 piRNAs 的特征：探索它们在三倍体不育中的潜在作用

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-07-29 DOI: 10.1007/s10126-024-10351-7

Yaru Zhou, Hong Yu, Qi Li, Lingfeng Kong, Shikai Liu, Chengxun Xu

{"title":"Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility","authors":"Yaru Zhou, Hong Yu, Qi Li, Lingfeng Kong, Shikai Liu, Chengxun Xu","doi":"10.1007/s10126-024-10351-7","DOIUrl":"10.1007/s10126-024-10351-7","url":null,"abstract":"<div>PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements, germ cell development, and gametogenesis. Triploid Pacific oysters (Crassostrea gigas) are vital in the oyster aquaculture industry due to reduced fertility and rapid growth. This study integrates piRNA and mRNA expression analyses to elucidate their potential contributions to the sterility of triploid C. gigas. Bioinformatics analysis reveals a distinct U-bias at the 5′ terminal of oyster piRNAs. The abundance of piRNA clusters is reduced in triploid gonads compared to diploid gonads, particularly in sterile gonads, with a significant decrease in piRNA numbers. A specific piRNA cluster is annotated with the PPP4R1 gene, which is downregulated in infertile female triploids and exhibits a negative correlation with three piRNAs within the cluster. Differential expression analysis identified 46 and 88 piRNAs in female and male comparison groups, respectively. In female sterile triploids, the expression of three target genes of differentially expressed piRNAs associated with cell division showed downregulation, suggesting the potential roles of piRNAs in the regulation of cell division-related genes, contributing to the gonad arrest observed in female triploid oysters. In male triploid oysters, piRNAs potentially interact with the target genes associated with spermatogenesis, including TSSK4, SPAG17, and CCDC81. This study provides a concise overview of piRNAs expression in oyster gonads, offering insights into the regulatory role of piRNAs in triploid sterility.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-Functional Activity of Pyrone Derivatives for Inhibition of Barnacle Settlement and Biofilm Formation 吡喃酮衍生物在抑制藤壶沉降和生物膜形成方面的结构-功能活性

IF 2.6 3区生物学

Marine Biotechnology Pub Date : 2024-07-27 DOI: 10.1007/s10126-024-10349-1

Mo Aqib Raza Khan, Bo-Wei Wang, Hsiu-Chin Lin, Yu-Liang Yang, Chih-Chuang Liaw

{"title":"Structure-Functional Activity of Pyrone Derivatives for Inhibition of Barnacle Settlement and Biofilm Formation","authors":"Mo Aqib Raza Khan, Bo-Wei Wang, Hsiu-Chin Lin, Yu-Liang Yang, Chih-Chuang Liaw","doi":"10.1007/s10126-024-10349-1","DOIUrl":"10.1007/s10126-024-10349-1","url":null,"abstract":"<div>Naturally occurring 6-pentyl-2H-pyran-2-one and its synthetic analogues greatly inhibit the settlement of Amphibalanus amphitrite cyprids and the growth and biofilm formation of marine bacteria. To optimize the antifouling activities of pyrone derivatives, this study designed pyrone analogues by modifying functional groups, such as the benzyl group, cyclopentane, and halides, substituted on both sides of a pyrone. The antifouling effects of the synthesized pyrone derivatives were subsequently evaluated against five marine biofilm–forming bacteria, Loktanella hongkongensis, Staphylococcus cohnii, S. saprophyticus, Photobacterium angustum, and Alteromonas macleodii, along with barnacle cyprids of Amphibalanus amphitrite. Substituting nonpolar parts—such as the aliphatic, cyclopentyl, or phenyl moieties on C-5 or the furan moieties on C-3—not only increased antibacterial activity and inhibited biofilm formation but also inhibited barnacle cyprid settlement when compared to 6-pentyl-2H-pyran-2-one.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141772415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0