Marine Biotechnology最新文献

{"title":"Genome-wide Association Study Reveals Novel Growth-related SNPs and Candidate Genes in Grass Carp (Ctenopharyngodon idella) Adapted to Hyper-salinity Stress","authors":"Jing Tian,&nbsp;Tao Zhu,&nbsp;Shengjie Li,&nbsp;Caixia Lei,&nbsp;Jinxing Du,&nbsp;Hongmei Song","doi":"10.1007/s10126-026-10600-x","DOIUrl":"10.1007/s10126-026-10600-x","url":null,"abstract":"<div>\u0000 \u0000 <p>Given the increasing saturation of freshwater aquaculture space, saline-alkali aquaculture has become a critical direction to expand fishery space. Grass carp (<i>Ctenopharyngodon idellus</i>) is an ideal candidate for saline-alkali aquaculture because of its strong osmoregulation ability. However, the mechanism and genetic basis of salt tolerance are unclear, which restricts the breeding efficiency of salt-tolerant and fast-growing strains. In the present study, body weight was used as a key indicator to measure growth traits, and 98 individuals of maximal and minimal grass carp under hypersaline stress were collected for the first time for a genome-wide association study. The results showed that 14 SNPs were significantly correlated with the body weight of grass carp under high-salinity stress, and intron mutations were the main type of variation. The most significant SNP is upstream of the UBL5 gene. Notably, three SNPs were located in the intron region of the GNG7 gene, which might regulate grass carp growth by regulating cell proliferation and differentiation, transmembrane signal transduction, and immune processes. The results of genotyping verification of UBL5, GNG7 and SLC16A2 further showed that there were significant differences between extremely large and extremely small individuals. In addition, the candidate genes were significantly involved in growth regulation, signal transduction and osmotic regulation-related pathways. In conclusion, this work will help to elucidate the genetic basis associated with fast-growth traits under a hyperosmotic environment, and the identified SNPs and candidate genes can be used for genomic selection breeding of salt-tolerant fast-growing strains in grass carp.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-Throughput Genotyping Workflow and an Optimized SSR Panel for High-Accuracy Parentage Analysis in Largemouth Black Bass","authors":"Kai Liu,&nbsp;Qinghui Meng,&nbsp;Tianlun Zheng,&nbsp;Nan Xie,&nbsp;Fan Zhou","doi":"10.1007/s10126-026-10602-9","DOIUrl":"10.1007/s10126-026-10602-9","url":null,"abstract":"<div>\u0000 \u0000 <p>Accurate pedigree information is paramount for genetic improvement programs in commercially important aquaculture species such as Largemouth black bass (<i>Micropterus salmoides</i>). Simple Sequence Repeats (SSRs) are highly polymorphic markers well-suited for parentage analysis, yet their application has been hampered by labor-intensive traditional genotyping methods. This study reports the development, validation, and optimization of a high-throughput SSR genotyping system for Largemouth black bass. We designed six 10-plex SSR panels, comprising 60 markers, by integrating MultiplexSSR for efficient primer design, Hi-TOM sequencing for targeted amplicon generation, and the SSRseq pipeline for automated and accurate genotyping. The developed SSRs exhibited moderate polymorphism (mean polymorphic information content, PIC = 0.396; mean number of alleles, <i>N</i>a = 2.48). Theoretical assignment power (<i>P</i>_u) analyses indicated that combinations of ≥ 30 markers achieved <i>P</i>_u values approaching 1.000 under ideal conditions. Empirical assignment accuracy mirrored this, exceeding 95% with ≥ 30 markers when no additional candidate parents were considered. While both<i> P</i>_u and accuracy were impacted by an increasing number of potential parents, a critical finding was that the targeted removal of eight problematic loci—identified via rigorous quality control thresholds for null allele frequencies and Mendelian inconsistencies—restored assignment accuracy from 90.77% to 100.00% in the in-sample validation. This retrospective optimization highlights the substantial impact of genotyping errors on parentage inference, although out-of-sample validation across independent cohorts is required. This resultant optimized 52-marker panel provides a robust tool, demonstrating that marker quality is a decisive factor for efficient, scalable, and precise SSR-based parentage assignment in Largemouth black bass.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Sargassum polycystum Enrichment in Feed on Immune Response and Survival Rate of Litopenaeus vannamei Against Vibrio harveyi Infection","authors":"Siti Subaidah,&nbsp;Desy Sugiani,&nbsp;Daniar Kusumawati,&nbsp;Joko Sumarwan,&nbsp;Indah Mastuti,&nbsp;Muhammad Marzuqi,&nbsp;Bejo Slamet,&nbsp;Suko Ismi,&nbsp; Tridjoko,&nbsp;Warih Hardanu,&nbsp;Lisa Ruliaty,&nbsp;Ketut Mahardika,&nbsp;Andi Parengrengi,&nbsp;Darmawan Setia Budi","doi":"10.1007/s10126-026-10599-1","DOIUrl":"10.1007/s10126-026-10599-1","url":null,"abstract":"<div>\u0000 \u0000 <p>Prior studies on <i>Sargasum</i> sp. as an immunostimulant were mostly focused on injection and immersion methods. Moreover, large-scale aquaculture requires practical applications to minimize stress caused by handling. The objective of this research was to investigate the effect of feed enrichment with extract of <i>Sargassum polycystum</i> on the immune response and survival rate of <i>Litopenaeus vannamei</i>. This study used <i>S. polycystum</i> which is a brown seaweed that contains bioactive compounds such as flavonoids and phenolics, was added to shrimp feed at varying concentrations (5, 10, and 15 g/kg). Immune parameters, including Total Hemocyte Count (THC), Differential Hemocyte Count (DHC), and immune gene expression (lectin and proPO) were analysed before and after the challenge test using <i>Vibrio harveyi</i>. The results showed that <i>S. polycystum</i> extract significantly increased THC, DHC, and immune gene expression compared to the control group, indicating an increase in immune defense. However, the enrichment did not significantly affect on shrimp growth. Post challenge test, the highest survival rate (80.56%) obtained in shrimp were fed with 10 g/kg <i>S. polycystum</i> extract. The results showed that dietary supplementation with extract of <i>S. polycystum</i> enhanced the immune system of <i>L. vannamei</i> and providing a preventive approach against pathogen infection.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Mining and Functional Characterization of Agarases from a Novel Vibrio Species","authors":"Marc Jeremie D. Punzalan,&nbsp;Neil Andrew D. Bascos,&nbsp;Arturo O. Lluisma","doi":"10.1007/s10126-026-10597-3","DOIUrl":"10.1007/s10126-026-10597-3","url":null,"abstract":"<div><p>Marine bacteria produce diverse carbohydrate-active enzymes (CAZymes), which include agarases that cleave glycosidic bonds in agars (agarans). Agars, sulfated polysaccharides that mostly make up the cell walls and intercellular matrix in many species of red algae, are used in various industrial applications. The discovery of novel agarases is thus of biological and biotechnological interest. In this study, the CAZymes and, in particular, the agarases of a seaweed-associated <i>Vibrio</i> sp. isolate, an agarolytic bacterium isolated from a macroalga, were characterized using genomic and phenotypic approaches. A draft genome assembly of <i>Vibrio</i> sp. A37 was generated using high-throughput short-read sequencing. The draft genome assembly comprised approximately 5 megabases (Mb) organized into 78 contigs, with an N50 of 381 kilobases (kb), GC content of 41%, and a read coverage of 209x. Genome-based comparisons show that strain A37 exhibits substantial divergence from its nearest described species, <i>Vibrio marisflavi</i>, with values of 78.93% ANI, 86.89% AAI, and 22.4% dDDH—well below the thresholds generally accepted for species delineation. Together with corroborating phenotypic data, these observations support the recognition of strain A37 as a novel genomospecies, for which we propose the provisional name ‘<i>Vibrio bolinaoensis</i>’. Analysis of the carbohydrate-active enzyme (CAZyme) repertoire revealed diverse CAZyme classes, including five β-agarases–four of which form a single CAZyme gene cluster (CGC). Further genomic analysis highlighted the isolate’s potential to degrade and utilize agarose, fucoidan, and alginate. Two β-agarase genes, Ag46 (a member of glycosyl hydrolase family 16 (GH16)) and Ag48 (GH50), were successfully expressed in <i>Escherichia coli</i> using the pCri-18a vector, originally designed for <i>Bacillus subtilis</i>. This led to the serendipitous discovery of the utility of this expression vector to enable secretion and extracellular activity of the agarases, and potentially other CAZymes, in <i>E. coli</i>, underscoring the fundamental mechanistic compatibility between the Sec-dependent translocation pathways of Gram-positive and Gram-negative bacteria.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide Association Study Identifies SNPs and Candidate Genes Associated with Abdominal Meat Weight and Meat Yield of red Swamp Crayfish Procambarus clarkii","authors":"Yongchuang Li,&nbsp;Qiujin Wang,&nbsp;Na Sheng,&nbsp;Zheyan Chen,&nbsp;Yongqing Wang,&nbsp;Liang Jia,&nbsp;Zhiyi Bai,&nbsp;Xilei Li,&nbsp;Jiale Li,&nbsp;Jianbin Feng","doi":"10.1007/s10126-026-10601-w","DOIUrl":"10.1007/s10126-026-10601-w","url":null,"abstract":"<div>\u0000 \u0000 <p>Red swamp crayfish (<i>Procambarus clarkii</i>) is an economically important freshwater species in the world. The abdominal meat weight (AMW) and meat yield (MY) are the principal economic traits of the species. To identify SNPs and candidate genes associated with AMW and MY of the species, a genome-wide association study (GWAS) was conducted to genotype 410 individuals using a 50 K liquid-phase breeding SNP array. A total of 46,367 high-quality SNPs was identified. The Mixed Linear Model (MLM), Fixed and random model Circulating Probability Unification (FarmCPU), and Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) models were identified to be suitable for GWAS analysis of AMW and MY of the species. Eleven significant SNPs were identified to be associated with AMW and ten SNPs with MY trait, respectively. Seven candidate genes (<i>FOXO</i>, <i>UBE4A</i>, <i>RAP2B</i>, <i>CCNO</i>,<i> CDK7</i>, <i>Mlp84B</i>, and <i>HK1</i>) were linked to AMW trait, while three candidate genes (<i>NDFIP1</i>, <i>PRKG1</i>, and <i>ADCY5</i>) were associated with MY trait based on KEGG and GO enrichment analyses. These candidate genes are primarily involved in biological processes including cell cycle arrest, apoptosis, oxidative stress response, and metabolic homeostasis. The results would benefit to understanding the genetic basis of abdominal meat weight and meat yield traits and providing potential molecular markers for genetic improvement of crayfish.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Leucine Requirement and Its Effects on Growth, Immunity, Antioxidant Capacity and Protein Metabolism in Pacific White Shrimp, Penaeus vannamei","authors":"Sanghyun Song,&nbsp;Suhyeok Kim,&nbsp;Yeonji Lee,&nbsp;Sua Kim,&nbsp;Yein Lee,&nbsp;Wonhoon Kim,&nbsp;Minju Kim,&nbsp;Hosung Jung,&nbsp;Dohoon Kim,&nbsp;Kyeong-Jun Lee","doi":"10.1007/s10126-026-10598-2","DOIUrl":"10.1007/s10126-026-10598-2","url":null,"abstract":"<div>\u0000 \u0000 <p>This study was conducted to determine an optimal dietary leucine (Leu) level for <i>Penaeus vannamei</i> by assessing growth, non-specific immune response, antioxidant capacity, digestive enzyme activity and protein metabolism-related gene expression. A basal (L0) diet was formulated without supplemental Leu. Five other diets were formulated supplementing Leu into L0 diet at 5, 10, 15, 20 and 25 g/kg (designated as L5, L10, L15, L20 and L25, respectively). Total of 480 shrimp were randomly distributed into 24 tanks (240 L) in quadruplicate groups per dietary treatment. Growth performance was significantly higher in L5 and L15 groups than in L0 group. Non-specific immune responses were significantly increased in Leu-supplemented groups than in L0 group. Muscle Leu concentration was significantly higher in all Leu-supplemented groups compared with L0 group. Catalase activity was significantly higher in L20 group than in L0 group. Glutathione peroxidase activity was significantly increased in L10 group than in L0 group. Superoxide dismutase activity was significantly increased in all Leu-supplemented groups than in L0 group. Compared to L0 group, dietary Leu supplementation significantly decreased the level of malondialdehyde. Activities of trypsin and lipase were significantly higher in all Leu-supplemented groups than in L0 group. Chymotrypsin activity was significantly higher in L10 group than in all other groups. All Leu-supplemented groups exhibited higher relative gene expression of <i>TOR</i>, <i>S6K</i> and <i>Nrf2</i> compared to L0 group. <i>4E-BP</i> was significantly upregulated in L5, L10, L20 and L25 groups than in L0 group. These results indicate that the dietary Leu requirement for maximum weight gain of <i>P. vannamei</i> is 10.6 g/kg.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Analysis of mRNA-seq and miRNA-seq Reveal the Dynamics of the Gonadal Differentiation of Procypris Mera","authors":"Zhenling Ke,&nbsp;Weijun Wu,&nbsp;Zhe Li,&nbsp;Yusen Li,&nbsp;Yaoquan Han,&nbsp;Jun Shi,&nbsp;Lilong Chen,&nbsp;Kangqi Zhou,&nbsp;Dapeng Wang,&nbsp;Yong Lin,&nbsp;Min Li,&nbsp;Hua Ye","doi":"10.1007/s10126-026-10581-x","DOIUrl":"10.1007/s10126-026-10581-x","url":null,"abstract":"<div>\u0000 \u0000 <p>The Chinese ink carp (<i>Procypris mera</i>), a primitive species within the Cyprinidae family, exhibits sexual dimorphism in growth, with females growing significantly faster than males. Due to the depletion of wild resources and limitations in breeding technology, its commercial viability has become concerning. Research into all-female breeding and germplasm resource recovery is crucial for addressing these challenges, with sex control intervention playing a pivotal role. In this study, we conducted histological observations of <i>P. mera</i> gonads using Hematoxylin-Eosin (HE) staining and identified four key stages of gonadal differentiation (60 dph, 95 dph, 110 dph, and 150 dph, ) for transcriptome sequencing. Additionally, miRNA sequencing was performed at the late differentiation stage (150 dph). Our results indicate that gonadal differentiation in <i>P. mera</i> is largely completed by 150 dph. During ovarian differentiation, the estrogen synthesis rate-limiting gene <i>cyp19a1a</i> is promoted by <i>foxl2</i>, facilitating estrogen production to drive ovarian differentiation while antagonizing male pathway genes. In contrast, during testicular differentiation, genes such as <i>dmrt1</i>, <i>sox9-b</i>, and <i>fgf1</i> are upregulated across all four stages, inhibiting the expression of female pathway genes dominated by <i>foxl2</i> and <i>cyp19a1a</i> to ensure commitment to testis differentiation. Furthermore, miRNAs also play a critical role in gonadal differentiation and maturation during the later stages. The miR-200 family regulates multiple genes associated with gonadal differentiation, potentially serving as core regulators. Additionally, some newly identified miRNAs (novel-m0207, novel-m0123-5P, and novel-m0122-5P) may also contribute to the regulation of gonadal differentiation processes. Overall, this study may provide valuable insights into the gonadal differentiation process and its underlying molecular regulatory network in <i>P. mera</i>. These findings offer a foundation for improving production performance and advancing aquaculture practices for this species.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147441675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Hierarchical Transcriptional Control of Fatty Acid Metabolism in Megalobrama Amblycephala: Insights from Promoter Architecture, PPAR Synergy, and Network Biology","authors":"Qixiang Wang,&nbsp;Wangcheng Haoyang,&nbsp;Junhan Luo,&nbsp;Yanling Qing,&nbsp;Yanan Luo,&nbsp;Xing Gao,&nbsp;Jing Liu,&nbsp;Zhenfeng Chen,&nbsp;Yanfang Li,&nbsp;Suchun Liu","doi":"10.1007/s10126-026-10596-4","DOIUrl":"10.1007/s10126-026-10596-4","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;p&gt;This study provides a comprehensive, multi-level analysis of the transcriptional regulatory architecture controlling key enzymes in the fatty acid elongation and desaturation pathways of &lt;i&gt;Megalobrama amblycephala&lt;/i&gt;, an economically significant freshwater aquaculture species. The research integrates in silicopromoter analysis, functional validation through dual-luciferase reporter assays, and systems-level transcriptional regulatory network (TRN) modeling to elucidate the complex mechanisms governing lipid metabolism. Our investigation focused on the promoter regions of five crucial genes—&lt;i&gt;ELOVL5&lt;/i&gt;, &lt;i&gt;ELVL2&lt;/i&gt;, &lt;i&gt;FADS2&lt;/i&gt;, &lt;i&gt;ELOVL4A&lt;/i&gt;, and &lt;i&gt;ELOVL4B&lt;/i&gt;—which encode enzymes responsible for determining fatty acid chain length and saturation levels, critically influencing cellular membrane composition, energy storage, and signaling molecule precursors . Computational dissection of the approximately 2.0–2.5 kb promoter regions revealed distinct and complex cis-regulatory landscapes for each gene, with unique enrichments for specific transcription factor binding motifs. The &lt;i&gt;ELOVL5&lt;/i&gt;promoter showed significant enrichment for Sp1-family factors (particularly Sp1.5), while the &lt;i&gt;ELOVL2&lt;/i&gt;promoter exhibited a predominance of GATA-1.1 binding sites. Analysis of the &lt;i&gt;ELOVL4&lt;/i&gt;paralogs revealed divergent evolutionary trajectories: &lt;i&gt;ELOVL4A&lt;/i&gt;displayed the highest overall cis-element density with clusters of sites for Sp1.4, EGR1, and GATA-1.1, whereas &lt;i&gt;ELOVL4B&lt;/i&gt;was overwhelmingly dominated by Elk-1.1 sites. The &lt;i&gt;FADS2&lt;/i&gt;promoter was characterized by a prevalence of CACCC-box binding factors. This bioinformatic mapping provided strong evidence for complex, gene-specific regulatory logic and identified potential peroxisome proliferator-activated receptor response elements (PPREs) within these promoter contexts . Functional validation using dual-luciferase reporter assays in HEK293T cells demonstrated that all five promoters are direct transcriptional targets of both PPARA and PPARG, with individual overexpression of either transcription factor inducing statistically significant transactivation (&lt;i&gt;p&lt;/i&gt; &lt; 0.0001). Notably, we identified a gene-specific hierarchy of co-regulation: PPARA and PPARG acted synergistically on the &lt;i&gt;ELOVL5&lt;/i&gt;, &lt;i&gt;ELOVL2&lt;/i&gt;, and &lt;i&gt;FADS2&lt;/i&gt;promoters, with combined overexpression resulting in luciferase activity significantly exceeding the sum of individual effects (&lt;i&gt;p&lt;/i&gt; &lt; 0.001). In contrast, their regulation of the &lt;i&gt;ELOVL4&lt;/i&gt;paralogs was merely additive or non-synergistic, suggesting distinct regulatory paradigms for different branches of the fatty acid metabolic network. This differential regulation may reflect promoter-intrinsic properties that determine the cooperative potential of PPARA and PPARG, possibly through mechanisms involving complex stabilization or recruitment of shared co-activators . At a systems level, we reconstructed a genom","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Noninvasive Epidermal Mucus RNA-Seq Analysis for Developing Methods to Evaluate Environmental Stress in the Japanese Eel (Anguilla japonica)","authors":"Motoshige Yasuike,&nbsp;Taiga Asakura,&nbsp;Yoji Nakamura,&nbsp;Yuki Hongo,&nbsp;Nobuto Fukuda","doi":"10.1007/s10126-026-10582-w","DOIUrl":"10.1007/s10126-026-10582-w","url":null,"abstract":"<div>\u0000 \u0000 <p>Environmental changes in rivers caused by fluctuations in water temperature and increasingly intense rainfall linked to climate change are likely to influence the physiological condition (environmental stress) of wild Japanese eels (<i>Anguilla japonica</i>). This study examined the epidermal mucus of <i>A. japonica</i>, which can be collected noninvasively in the field, and evaluated whether RNA-Seq analysis could characterize the physiological state of this species in response to such environmental stressors. We conducted controlled tank trials simulating (1) suspended solids (SS) stress resulting from sediment runoff during heavy rainfall (using kaolin clay addition) and (2) water temperature variations (10 °C, 20 °C, and 30 °C) as physical stresses experienced by wild <i>A. japonica</i> under climate-driven changes. Subsequently, we performed RNA-Seq analysis to determine genes in the epidermal mucus responsive to each experimental stressor. As a result, under (1) SS stress, genes associated primarily with cellular immunity and inflammatory pathways showed elevated expression. In the (2) water temperature trials, relative to the control (20 °C), the low-temperature group (10 °C) displayed increased expression of lipid-metabolism-related genes such as <i>scd</i>, <i>prom2</i>, and <i>tmem64</i>, whereas the high-temperature group (30 °C) exhibited heightened expression of genes linked to the heat-shock response. Thus, because this study enabled the identification of genes specifically expressed in reaction to each stressor from the epidermal mucus of <i>A. japonica</i>, these findings are expected to support the future development of noninvasive approaches for evaluating the physiological state (health condition) of wild <i>A. japonica</i>.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12971777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Isolation and Identification of High Biomass and Lipid Productivity Euglena Strain from Tropical Malaysian Environments for Enhancement of Biofuel Production","authors":"Sabrina Aghazada,&nbsp;Kengo Suzuki,&nbsp;Yu Inaba,&nbsp;Kohei Atsuji,&nbsp;Koji Iwamoto","doi":"10.1007/s10126-026-10574-w","DOIUrl":"10.1007/s10126-026-10574-w","url":null,"abstract":"","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-026-10574-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}