Marine Biotechnology最新文献_第10页

Immunoinformatics-Based Design of a Multiple-Epitope Vaccine Against IHNV 基于免疫信息学的IHNV多表位疫苗设计

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-11 DOI: 10.1007/s10126-026-10621-6

Najib Abdellaoui, Jae-Woong Lim, Hyun-Ja Han, Mi Young Cho, Min Sun Kim

{"title":"Immunoinformatics-Based Design of a Multiple-Epitope Vaccine Against IHNV","authors":"Najib Abdellaoui, Jae-Woong Lim, Hyun-Ja Han, Mi Young Cho, Min Sun Kim","doi":"10.1007/s10126-026-10621-6","DOIUrl":"10.1007/s10126-026-10621-6","url":null,"abstract":"<div><p>Infectious hematopoietic necrosis virus (IHNV) is a significant viral pathogen that affects salmonids, leading to high mortality and substantial economic losses in aquaculture. The current vaccine strategies focus on use of DNA and inactivated vaccines. However, these strategies face limitations concerning biosafety and efficacy. Therefore, in this work, we employed an approach based on the immunoinformatic platform to develop a multi-epitope vaccine against IHNV. For that purpose, we analyzed the glycoprotein of IHNV and identified highly antigenic and non-allergenic cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL), and B-cell epitopes. The different epitopes were assembled with rational linkers, and the N-terminal flagellin FliC adjuvant was added to enhance immunogenicity. The designed vaccine showed favorable physicochemical properties and high structural stability, which was validated through modeling and refinement. Moreover, the molecular docking of the designed vaccine with toll-like receptor 5 (TLR5) and molecular dynamics simulation revealed stable and strong interactions between the vaccine and TLR5, demonstrating that the designed vaccine can activate innate immunity. Furthermore, in silico immune simulations demonstrate a robust humoral and cellular immune response following multiple doses. These findings provide a promising framework for the development of a novel, safe, and effective vaccine against the IHNV infection in Atlantic salmon.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application and Effects of GDF9 as a Novel Inductive Additive on Early Ovarian Development in Japanese Eel (Anguilla japonica) 新型诱导添加剂GDF9在日本鳗鲡卵巢早期发育中的应用及影响

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-11 DOI: 10.1007/s10126-026-10624-3

Chenpeng Zuo, Xuanhan Zhang, Tianyu Jiang, Jing Yang, Ming Liu, Mingchun Wu, Chengcheng Zhang, Haishen Wen, Xin Qi

{"title":"Application and Effects of GDF9 as a Novel Inductive Additive on Early Ovarian Development in Japanese Eel (Anguilla japonica)","authors":"Chenpeng Zuo, Xuanhan Zhang, Tianyu Jiang, Jing Yang, Ming Liu, Mingchun Wu, Chengcheng Zhang, Haishen Wen, Xin Qi","doi":"10.1007/s10126-026-10624-3","DOIUrl":"10.1007/s10126-026-10624-3","url":null,"abstract":"<div><p><i>Anguilla japonica</i> (Japanese eel) is a commercially valuable aquaculture species, yet large-scale artificial reproduction remains a major unresolved challenge. This study evaluated the potential of growth differentiation factor 9 (GDF9), an oocyte-derived regulator, as a supplementary additive during hormonally induced ovarian development in Japanese eel. Compared with treatment using gonadotropin analogs alone, co-administration of exogenous recombinant GDF9 significantly enhanced early ovarian development, as indicated by an increased gonadosomatic index (GSI), improved follicular growth, and elevated serum estradiol (E2) and vitellogenin (VTG) levels. Transcriptomic analysis showed that differentially expressed genes (DEGs) were significantly enriched in biological processes and pathways related to EGF signaling, angiogenesis, cell proliferation, transmembrane transport, and reproductive signaling, including the GnRH, FoxO, and Apelin pathways. Metabolomic profiling further revealed significant changes in metabolites associated with carbohydrate metabolism, amino acid biosynthesis and metabolism, and steroid-related pathways. Integrated analysis demonstrated coordinated correlations between key DEGs and differential metabolites, particularly in pathways involved in steroidogenesis, nutrient transport, and amino acid metabolism. These findings suggest that GDF9 may promote early ovarian development through coordinated regulation of gene expression and metabolic activity. As a supplementary molecular additive, GDF9 shows promise for improving hormone-based induction strategies in Japanese eel and may provide useful insights for enhancing artificial reproduction in other teleost species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptome and Gene-Editing Analyses Reveal the Molecular Mechanism of Albinism in the Taiwanese Loach 比较转录组和基因编辑分析揭示台湾泥鳅白化病的分子机制。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-11 DOI: 10.1007/s10126-026-10628-z

Rongyun Li, Xiaohui Bai, Xiaojuan Cao, Shuang Hao, Keming Liu, Zhang Luo, Jian Gao, Yaoxin Wang, Yunbang Zhang, Lin Ma, Jufeng Jiang

{"title":"Comparative Transcriptome and Gene-Editing Analyses Reveal the Molecular Mechanism of Albinism in the Taiwanese Loach","authors":"Rongyun Li, Xiaohui Bai, Xiaojuan Cao, Shuang Hao, Keming Liu, Zhang Luo, Jian Gao, Yaoxin Wang, Yunbang Zhang, Lin Ma, Jufeng Jiang","doi":"10.1007/s10126-026-10628-z","DOIUrl":"10.1007/s10126-026-10628-z","url":null,"abstract":"<div><p>The Taiwanese loach (<i>Paramisgurnus dabryanus</i> ssp. <i>Taiwanese</i>), known for its nutritional and ornamental qualities, exhibits a rare form of albinism. Specifically, certain individuals display golden-pink eyes and a golden-red body color devoid of blotches, thereby attracting consumers. However, the principal genes regulating this phenotype remain unidentified. Consequently, this study conducted a transcriptome comparative analysis of the skin between wild-type (M) and mutant (N) Taiwanese loaches. A total of 6,152 differentially expressed genes (DEGs) were identified, comprising 2,467 down-regulated and 3,685 up-regulated DEGs in mutant individuals. Additionally, several pigmentation-related genes (<i>mitf</i>, <i>wnt3</i>, <i>mc1r</i>, <i>wnt1</i>, <i>dct</i>, <i>tyrp1</i>, and <i>tyr</i>) were identified. KEGG enrichment analysis of DEGs revealed pathways associated with melanin deposition, including the melanogenesis, tyrosine metabolism, and folate biosynthesis pathways. Eleven DEGs were randomly selected to validate RNA-seq results through qPCR. Furthermore, key genes (<i>tyr</i>, <i>mc1r</i>) were validated through gene knockout, accurately identifying the main gene responsible for color mutation in the Taiwanese loach. This study not only offers new insights into the molecular mechanism underlying abnormal body color in the Taiwanese loach but also demonstrates that gene knockout technology can rapidly produce a large quantity of golden-red loaches, significantly enhancing their economic value.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Resequencing Reveals Selection Signals Associated with Vibrio parahaemolyticus Stress Tolerance in Portunus trituberculatus 全基因组重测序揭示了与副溶血性弧菌应激耐受性相关的选择信号。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-09 DOI: 10.1007/s10126-026-10629-y

Dongfang Sun, Hangfeng Pan, Yichen Su, Jiayi Pan, Xianfa Zhou, Baoquan Gao, Jianjian Lv

{"title":"Whole-Genome Resequencing Reveals Selection Signals Associated with Vibrio parahaemolyticus Stress Tolerance in Portunus trituberculatus","authors":"Dongfang Sun, Hangfeng Pan, Yichen Su, Jiayi Pan, Xianfa Zhou, Baoquan Gao, Jianjian Lv","doi":"10.1007/s10126-026-10629-y","DOIUrl":"10.1007/s10126-026-10629-y","url":null,"abstract":"<div><p>Acute hepatopancreatic necrosis disease (AHPND) is a fatal disease caused by <i>Vibrio parahaemolyticus</i>, which severely impacts the crustacean aquaculture industry. In this study, we focused on the economically important <i>Portunus trituberculatus</i>, and investigated the differences in disease resistance among domesticated populations and its genetic basis using artificial infection experiments and resequencing analysis. A 12-day <i>V</i>. <i>parahaemolyticus</i> (5.6 × 10<sup>6</sup> CFU/mL) challenge experiment involving 1,000 crabs identified a subset of highly resistant individuals. Whole-genome resequencing of the high-resistant and normal populations generated a total of 1,261 Gb of sequencing data, achieving an average depth of 14.43×. Population structure and selective sweep analyses identified 96 candidate regions and 244 candidate genes, including <i>Serpin B1</i> and <i>Croquemort</i>. KEGG enrichment analysis revealed that the candidate genes were significantly enriched in signaling pathways related to bacterial infections, including <i>Salmonella</i> infections, bacterial invasion of epithelial cells and phagolysosomes. Notably, genes from the actin family emerged as central components within these pathways, suggesting their pivotal role in mediating immune responses in the highly resistant population. These findings provide exploratory genomic evidence and candidate resources for future functional validation and disease-resistant breeding.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

White Adipose Tissue as a Functional Target of Secondary Bile Acids in Rainbow Trout (Oncorhynchus mykiss) 虹鳟鱼（Oncorhynchus mykiss）中白色脂肪组织作为次生胆汁酸的功能靶点。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-09 DOI: 10.1007/s10126-026-10622-5

Gabriel Pérez-Tierra, Jessica Calo, Ayelén M. Blanco, José L. Soengas

{"title":"White Adipose Tissue as a Functional Target of Secondary Bile Acids in Rainbow Trout (Oncorhynchus mykiss)","authors":"Gabriel Pérez-Tierra, Jessica Calo, Ayelén M. Blanco, José L. Soengas","doi":"10.1007/s10126-026-10622-5","DOIUrl":"10.1007/s10126-026-10622-5","url":null,"abstract":"<div><p>Secondary bile acids (SBAs) are increasingly recognized as hormone-like signals in vertebrates, yet their regulatory potential in fish adipose tissue remains largely unexplored. This study evaluated the acute effects of SBAs (500 µM lithocholic acid -LCA-, 1500 µM deoxycholic acid -DCA-, and their taurine conjugates: 1000 µM T-LCA and 600 µM T-DCA) on the transcriptomic profile, glucose and lipid homeostasis, and bile acid (BA)–related pathways in rainbow trout white adipose tissue (WAT) six hours after intragastric administration. The main results showed that LCA emerged as the main metabolic modulator, enhancing glycolytic entry points via increased hexokinase and pyruvate kinase activities, modulating energy-sensing genes <i>prkaa1/2</i> (Ampk) and <i>mtor</i> (mTOR), and promoting coordinated adjustments in lipid metabolism, including upregulation of <i>acly</i> and <i>hadh</i> (Hoad), increased Acly activity, and reduced NEFA levels suggesting a potential enhancement of metabolic flexibility and intracellular lipid turnover. In contrast, DCA induced a more restricted response, upregulating mRNA abundance of genes encoding the BA transporters Ostα1 and Ostβ, the receptor FXRα-1, and Cyp8b1 isoforms, suggesting a targeted role in local BA sensing and signaling. Taurine-conjugated SBAs generally produced attenuated effects, with T-DCA tending to promote lipid retention and T-LCA evoking minimal changes. Overall, this study provides the first evidence of BA–related elements in rainbow trout WAT and suggests that SBAs can potentially modulate adipose tissue function in a manner dependent on BA identity, laying the foundation for future studies on their physiological and nutritional roles.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13157367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Split Aptamer-based Lateral Flow Dipstick for the Detection of Phaeocystis globosa 基于分裂适体的球形褐囊藻横向流动检测尺的建立

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-05-07 DOI: 10.1007/s10126-026-10623-4

Xueyang Tian, Mingjie Zhang, Yixin Ma, Chunyun Zhang, Yuanyuan Wang, Changlu Guo, Guofu Chen

{"title":"Establishment of a Split Aptamer-based Lateral Flow Dipstick for the Detection of Phaeocystis globosa","authors":"Xueyang Tian, Mingjie Zhang, Yixin Ma, Chunyun Zhang, Yuanyuan Wang, Changlu Guo, Guofu Chen","doi":"10.1007/s10126-026-10623-4","DOIUrl":"10.1007/s10126-026-10623-4","url":null,"abstract":"<div><p><i>Phaeocystis globosa</i> is one of the major dominant species that cause harmful algae, and its massive reproduction will have a significant impact on marine ecosystems and aquaculture. Therefore, the development of sensitive, fast and portable detection methods is crucial for early identification of algae and follow-up risk assessment. Based on the split aptamer identification strategy, this study constructs a split aptamer-lateral flow dipstick (SA-LFD) platform for the detection of <i>P. globosa</i>, and systematically evaluates its analysis performance. The results show that the method is simple to perform and yields clearly visible results, with the caveat that sample pretreatment requires centrifugation. The system has high specificity for <i>P. globosa</i>, and no cross-reaction was detected in various non-target algae tested. The detection limit of the system is 1.35 × 10<sup>3</sup> cells mL<sup>− 1</sup>, and it also shows robustness in mixed algae communities and simulated natural seawater samples, indicating that it has a high resistance to matrix interference. The split aptamer strategy effectively reduced background signal interference and improved detection accuracy. Overall, this method integrates sensitivity, specificity, and portability, making it suitable for rapid laboratory-based screening and emergency assessment, and provides a feasible technical approach for the rapid detection of harmful algae and red tide early warning, with potential extension to the detection of other algal species after further simplification of sample pretreatment.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated Vitality Evaluation of Shrimp Postlarvae via Machine Vision: A Multi-Object Tracking and Behavioral Analytics Approach 基于机器视觉的虾幼虫活力自动评估：一种多目标跟踪和行为分析方法。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-04-28 DOI: 10.1007/s10126-026-10620-7

Hao Gu, Luxi Yu, Hongda Li, Ming Chen

{"title":"Automated Vitality Evaluation of Shrimp Postlarvae via Machine Vision: A Multi-Object Tracking and Behavioral Analytics Approach","authors":"Hao Gu, Luxi Yu, Hongda Li, Ming Chen","doi":"10.1007/s10126-026-10620-7","DOIUrl":"10.1007/s10126-026-10620-7","url":null,"abstract":"<div>\u0000 \u0000 <p>Activity of shrimp postlarvae is a critical proxy for quality and survival in aquaculture, yet current assessments rely on manual observation of swimming behavior, which is subjective and difficult to standardize. We introduce an automated, objective, and scalable video-analytics pipeline for evaluating activity in <i>Litopenaeus vannamei</i> postlarvae. The system first applies YOLOv8-Pose to extract keypoint-based positional data and body lengths from individual postlarvae across video frames. These detections are linked over time using the BoTSORT multi-object tracker to derive individual-level motion trajectories. From these trajectories, we construct an evaluation framework grounded in four population-level metrics—trajectory irregularity, uniformity, surface skimming ratio, and polarization—that jointly capture swarm dynamics and vertical distribution. We quantify the relative importance of these metrics via a hybrid weighting scheme combining the Analytic Hierarchy Process (AHP) with cluster-based collective decision-making, and fuse them into a comprehensive activity score through weighted aggregation of the machine-vision outputs. Finally, we stratify the resulting activity scores into three operational levels—high, moderate, and low—using K-means clustering. This approach replaces subjective inspection with a reproducible, data-driven assessment, enabling standardized monitoring and decision support in hatchery practice.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Target Genes for Engineered Genetic Incompatibility in Fish 鱼类工程遗传不亲和性靶基因的鉴定。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-04-25 DOI: 10.1007/s10126-026-10617-2

Leland Feist, Samuel E Erickson, Nicholas Jacob, Isabel Ameli, Carolyn Malecha, Julie Badger, Michael J Smanski

{"title":"Identifying Target Genes for Engineered Genetic Incompatibility in Fish","authors":"Leland Feist, Samuel E Erickson, Nicholas Jacob, Isabel Ameli, Carolyn Malecha, Julie Badger, Michael J Smanski","doi":"10.1007/s10126-026-10617-2","DOIUrl":"10.1007/s10126-026-10617-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Genetic biocontrol approaches promise to complement existing physical and chemical methods as part of integrated pest management (IPM) strategies for the control of aquatic invasive species (AIS). Engineered Genetic Incompatibility (EGI) is a strategy for producing organisms that are reproductively isolated from wild conspecifics and could be used as non-persistent genetic biocontrol agents. Previously successfully demonstrated in yeast and insects, here we report early-stage research and development results towards translating this approach from insects into fish. Using <i>Danio rerio</i> (zebrafish) as a model system, we report the identification of target genes and sequence-programmable transcriptional activators (PTAs) that are suitable for EGI development in fish and evaluate their performance <i>in vivo</i>, in a model organism. We also describe several challenges faced when integrating the component parts into a complete system capable of displaying complete genetic incompatibility with wild-type conspecifics. Lastly, we discuss the steps needed to translate EGI from a model fish species to a target invasive species such as common carp (<i>Cyprinus carpio</i>).</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and Metabolic Responses to Graded Dietary Biotin Supplementation in Juvenile Penaeus vannamei 饲料中生物素分级添加对凡纳滨对虾幼虾生理代谢的响应。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-04-25 DOI: 10.1007/s10126-026-10615-4

Sua Kim, Suhyeok Kim, Danbi Shin, Nalin Medagoda, Kyeong-Jun Lee

{"title":"Physiological and Metabolic Responses to Graded Dietary Biotin Supplementation in Juvenile Penaeus vannamei","authors":"Sua Kim, Suhyeok Kim, Danbi Shin, Nalin Medagoda, Kyeong-Jun Lee","doi":"10.1007/s10126-026-10615-4","DOIUrl":"10.1007/s10126-026-10615-4","url":null,"abstract":"<div>\u0000 \u0000 <p>Biotin serves as a coenzyme in carboxylation reactions and plays a fundamental role in supporting growth, nutrient metabolism and overall physiological function in aquatic animals. This study investigated the biochemical and metabolic responses of graded dietary biotin in <i>Penaeus vannamei</i>. Five experimental diets were prepared with graded biotin supplementation levels of 0, 2, 4, 6 and 8 mg/kg (designated as Con, B02, B04, B06 and B08, respectively). The corresponding analyzed biotin concentrations were 1.65, 3.76, 5.84, 8.14 and 10.41 mg/kg, respectively. Twenty shrimp (0.55 ± 0.01 g) were randomly stocked into each tank, with four replicate tanks assigned to each experimental diet. The shrimp were fed the diets six times daily for 6 weeks. Dietary biotin supplementation significantly increased growth and improved feed utilization and protein efficiency ratio. Survival was not affected by the dietary treatment during the feeding trial. Whole-body lipid decreased linearly with increasing dietary biotin concentration. Hepatopancreatic biotin concentration exhibited a significant linear increase with graded dietary biotin concentrations. Chymotrypsin, lipase and amylase activities and intestinal villi height showed significant quadratic responses to dietary biotin concentration. Lysozyme activity increased linearly with dietary biotin concentrations, while antiprotease activity was significantly elevated in B02, B04 and B06 groups compared to Con group. Superoxide dismutase activity was significantly elevated in all biotin-supplemented groups. Based on piece-wise regression analysis, the optimal dietary biotin concentration for growth and feed conversion ratio was estimated at 3.09 to 3.66 mg/kg, respectively.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Exosomes/Small Extracellular Vesicles: Evidence of Extracellular RNA Release by Aquatic Organisms 环境外泌体/细胞外小泡：水生生物细胞外RNA释放的证据。

IF 2.8 3区生物学

Marine Biotechnology Pub Date : 2026-04-25 DOI: 10.1007/s10126-026-10618-1

Ryo Yonezawa, Lingxin Meng, Naoki Hashimoto, Ibuki Igarashi, Satoshi Kimura, Nina Yasuda, Susumu Mitsuyama, Takanori Kobayashi, Kazutoshi Yoshitake, Shigeharu Kinoshita, Nahoko Bailey-Kobayashi, Kaoru Maeyama, Kiyohito Nagai, Shugo Watabe, Tetsuhiko Yoshida, Shuichi Asakawa

{"title":"Environmental Exosomes/Small Extracellular Vesicles: Evidence of Extracellular RNA Release by Aquatic Organisms","authors":"Ryo Yonezawa, Lingxin Meng, Naoki Hashimoto, Ibuki Igarashi, Satoshi Kimura, Nina Yasuda, Susumu Mitsuyama, Takanori Kobayashi, Kazutoshi Yoshitake, Shigeharu Kinoshita, Nahoko Bailey-Kobayashi, Kaoru Maeyama, Kiyohito Nagai, Shugo Watabe, Tetsuhiko Yoshida, Shuichi Asakawa","doi":"10.1007/s10126-026-10618-1","DOIUrl":"10.1007/s10126-026-10618-1","url":null,"abstract":"<div>\u0000 \u0000 <p>Aquatic organisms continuously interact with surrounding water, yet whether they release extracellular vesicles into this vast medium remains unknown. We hypothesized that pearl oysters (<i>Pinctada fucata</i>) release exosomes/small extracellular vesicles (sEVs) into the aquatic environment. To this end, we collected exosome/sEV-sized components by ultrafiltration from tank water and open-sea culture areas. Microscopy revealed abundant vesicles consistent with exosome/sEV size, and small RNA sequencing identified pearl oyster-specific PIWI-interacting RNAs (piRNAs) that matched sequences previously detected in hemolymph exosomes. These findings demonstrate that pearl oysters actively released exosomes containing species-specific nucleic acids into surrounding water, effectively protecting the RNA from rapid degradation. We propose referring to these vesicles as environmental exosomes/environmental sEVs (eExosomes/esEVs). These findings suggest that aquatic exosomes/sEVs serve as carriers of RNA and may contribute to inter-organismal communication networks. Beyond their functional role, eExosomes/esEVs also hold promise as highly stable, novel targets for environmental DNA/RNA (eDNA/eRNA) analysis, offering new opportunities for ecological monitoring and biodiversity research.</p>\u0000 </div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0