Marine Biotechnology最新文献

{"title":"Alleviating Coral Thermal Stress via Inoculation with Quorum Quenching Bacteria","authors":"Qingsong Yang,&nbsp;Bing Yang,&nbsp;Bin Yang,&nbsp;Wenqian Zhang,&nbsp;Xiaoyu Tang,&nbsp;Huiming Sun,&nbsp;Yanying Zhang,&nbsp;Jie Li,&nbsp;Juan Ling,&nbsp;Junde Dong","doi":"10.1007/s10126-024-10344-6","DOIUrl":"10.1007/s10126-024-10344-6","url":null,"abstract":"<div><p>In the background of global warming, coral bleaching induced by elevated seawater temperature is the primary cause of coral reef degradation. Coral microbiome engineering using the beneficial microorganisms for corals (BMCs) has become a hot spot in the field of coral reef conservation and restoration. Investigating the potential of alleviating thermal stress by quorum quenching (QQ) bacteria may provide more tools for coral microbial engineering remediation. In this study, QQ bacteria strain <i>Pseudoalteromonas piscicida</i> SCSIO 43740 was screened among 75 coral-derived bacterial strains, and its quorum sensing inhibitor (QSI) compound was isolated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). Then, the thermal stress alleviating potential of QQ bacteria on coral <i>Pocillopora damicornis</i> was tested by a 30-day controlled experiment with three different treatments: control group (Con: 29 °C), high temperature group (HT: 31 °C), and the group of high temperature with QQ bacteria inoculation (HTQQ: 31 °C + QQ bacteria). The results showed that QQ bacteria SCSIO 43740 inoculation can significantly mitigate the loss of symbiotic algae and impairment of photosynthesis efficiency of coral <i>P. damicornis</i> under thermal stress. Significant difference in superoxide dismutase (SOD) and catalase (CAT) enzyme activities between HT and HTQQ was not observed. In addition, QQ bacteria inoculation suppressed the coral microbial community beta-dispersion and improved the stability of microbial co-occurrence network under thermal stress. It was suggested that QQ bacteria inoculation can alleviate coral thermal stress via reshaping microbial interaction and maintain community stability of coral microbiome. This study provided new evidence for the probiotic function of QQ bacteria in corals, which shedding light on the development of new microbiological tools for coral reef conservation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"951 - 963"},"PeriodicalIF":2.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Gene Alternative Splicing Events on Resistance to Cryptocaryonosis of Large Yellow Croaker (Larimichthys crocea)","authors":"Xin Li,&nbsp;Qiaozhen Ke,&nbsp;Ang Qu,&nbsp;Jiaying Wang,&nbsp;Ji Zhao,&nbsp;Peng Xu,&nbsp;Tao Zhou","doi":"10.1007/s10126-024-10342-8","DOIUrl":"10.1007/s10126-024-10342-8","url":null,"abstract":"<div><p>Large yellow croaker (<i>L. crocea</i>) is a productive species in marine aquaculture with great economic value in China. However, the sustainable development of large yellow croaker is hampered by various diseases including cryptocaryonosis caused by <i>Cryptocaryon irritans</i>. The genetic regulation processes for cryptocaryonosis in large yellow croaker are still unclear. In this present study, we analyzed differential alternative splicing events between a <i>C. irritans</i> resistance strain (RS) and a commercial strain (CS). We identified 678 differential alternative splicing (DAS) events from 453 genes in RS and 719 DAS events from 500 genes in CS. A set of genes that are specifically alternatively spliced in RS was identified including <i>mfap5</i>, <i>emp1</i>, and <i>trim33</i>. Further pathway analysis revealed that the specifically alternative spliced genes in RS were involved in innate immune responses through the PRR pathway and the Toll and Imd pathway, suggesting their important roles in the genetic regulation processes for cryptocaryonosis in large yellow croaker. This study would be helpful for the studies of the pathogenesis of cryptocaryonosis and dissection of <i>C. irritans</i> resistance for <i>L. crocea.</i></p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"741 - 753"},"PeriodicalIF":2.6,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Single-Nucleotide Polymorphisms in Differentially Expressed Genes Favoring Soybean Meal Tolerance in Higher-Growth Zebrafish (Danio rerio)","authors":"Pilar E. Ulloa,&nbsp;Felipe Jilberto,&nbsp;Natalia Lam,&nbsp;Gonzalo Rincón,&nbsp;Luis Valenzuela,&nbsp;Valentina Cordova-Alarcón,&nbsp;Adrián J. Hernández,&nbsp;Patricio Dantagnan,&nbsp;Maria Cristina Ravanal,&nbsp;Sebastian Elgueta,&nbsp;Cristian Araneda","doi":"10.1007/s10126-024-10343-7","DOIUrl":"10.1007/s10126-024-10343-7","url":null,"abstract":"<div><p>Genetic variability within the same fish species could confer soybean meal (SBM) tolerance in some individuals, thus favoring growth. This study investigates the single-nucleotide polymorphisms (SNPs) in differentially expressed genes (DEGs) favoring SBM tolerance in higher-growth zebrafish (<i>Danio rerio</i>). In a previous work, nineteen families of zebrafish were fed a fish meal diet (100FM control diet) or SBM-based diets supplemented with saponin (50SBM + 2SPN–experimental diet), from juvenile to adult stages. Individuals were selected from families with a genotype-by-environment interaction higher (170 ± 18 mg) or lower (76 ± 10 mg) weight gain on 50SBM + 2SPN in relation to 100FM. Intestinal transcriptomic analysis using RNA-seq revealed six hundred and sixty-five differentially expressed genes in higher-growth fish fed 50SBM + 2SPN diet. In this work, using these results, 47 SNPs in DEGs were selected. These SNPs were genotyped by Sequenom in 340 zebrafish that were fed with a 50SBM + 2SPN diet or with 100FM diet. Marker-trait analysis revealed 4 SNPs associated with growth in 3 immunity-related genes (<i>aif1l</i>, <i>arid3c</i>, and <i>cst14b.2</i>) in response to the 50SBM + 2SPN diet (<i>p</i>-value &lt; 0.05). Two SNPs belonging to <i>aif1l</i> y <i>arid3c</i> produce a positive (+19 mg) and negative (−26 mg) effect on fish growth, respectively. These SNPs can be used as markers to improve the early selection of tolerant fish to SBM diet or other plant-based diets. These genes can be used as biomarkers to identify SNPs in commercial fish, thus contributing to the aquaculture sustainability.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"754 - 765"},"PeriodicalIF":2.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Investigation to Discriminate Sexes in Alive Nile Tilapia (Oreochromis niloticus) Using Cyp19a1a and Dmrt1 Gene Expression in Tail Fin Tissues","authors":"Samy Y. El-Zaeem,&nbsp;Amr El-Hanafy,&nbsp;Alaa A. El-Dahhar,&nbsp;Ayaat M. Elmaghraby,&nbsp;Amany M. Hendy","doi":"10.1007/s10126-024-10340-w","DOIUrl":"10.1007/s10126-024-10340-w","url":null,"abstract":"<div><p>The Nile Tilapia (<i>Oreochromis niloticus</i>), a gonochoristic teleost fish with a XX/XY sex-determination system, is an ideal model for investigating gonadal sex differentiation. During gonadal differentiation, the expression of <i>cyp19a1a</i> in XX gonads and <i>dmrt1</i> in XY gonads are required for undifferentiated tissues to develop into ovary or testis. In this study, quantitative real-time RT-PCR assessed the expression of <i>cyp19a1a</i> and <i>dmrt1</i> genes in gonads and tail fin tissues. Differences in gene expression mean among sexually differentiated fish were analyzed using two-way analysis of variance (ANOVA) and validation of mixed model using discriminant analysis (DA) for morphometric traits and the gene expression in gonads and tail fin tissues used to validate and utilize them in discriminating sexes in sex-differentiated Nile Tilapia fish. The results revealed that, <i>cyp19a1a</i> gene expression in female ovaries was more significant than <i>dmrt1</i> in male testis. In the other hand, the <i>dmrt1</i> gene expression in the tail fin was higher in males than females. Both, <i>cyp19a1a</i> and <i>dmrt1</i> genes, can discriminate fish sexes by 100% by using their expression in tail fin tissues. In conclusion, the <i>cyp19a1a</i> and <i>dmrt1</i> genes could be used as a genetic marker to discriminate between the Nile Tilapia sexes, whereas used as an indicator for ovarian or testis differentiation in sexually differentiated Nile Tilapia using tail fin tissues. It is worth mentioning that this is the first investigation for using <i>cyp19a1a</i> and <i>dmrt1</i> genes from Nile Tilapia tail fin tissues in sex determination.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"943 - 950"},"PeriodicalIF":2.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10340-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Selection of Large Yellow Croaker (Larimichthys crocea) with a High Plant Protein Diet Enhances the Growth Performance of Offspring","authors":"Qiaozhen Ke,&nbsp;Jiaxing Liu,&nbsp;Ji Zhao,&nbsp;Jiaying Wang,&nbsp;Pengxin Jiang,&nbsp;Yacheng Deng,&nbsp;Xiaoying Zhou,&nbsp;Junjia Zeng,&nbsp;Tao Zhou,&nbsp;Peng Xu","doi":"10.1007/s10126-024-10341-9","DOIUrl":"10.1007/s10126-024-10341-9","url":null,"abstract":"<div><p>Fishmeal is over-represented in the diets of large yellow croaker (<i>Larimichthys crocea</i>), and this farming mode, which relies heavily on fishmeal, is highly susceptible to the price of fishmeal and is unsustainable. Therefore, more and more studies on the large yellow croaker tend to replace fishmeal with land-based animal or plant proteins, but few studies have considered it from the genomic selection. In this study, we evaluated the survival rate (SR), final body weight (FBW), body weight gain (BWG), weight gain rate (WGR), and specific growth rate (SGR) of the large yellow croaker GS7 strain, which was obtained through genomic selection for tolerance to plant proteins and analyzed the differences in plant protein utilization between the GS7 strain and unselected commercial large yellow croaker (control group). The results of separate feeding for 60 days showed that although there was no significant difference in SR between the control and GS7 strains (<i>P</i> &gt; 0.05), the BWG, WGR, and SGR of the control were significantly lower (<i>P</i> &lt; 0.05) than those of the GS7 group. Results of mixed feeding after PIT marking showed that compared to the control fish, the GS7 strain had significantly higher BWG, WGR, and SGR (<i>P</i> &lt; 0.0001). To make the experimental results more precise, we compared fishes with equivalent initial body weight (IBW) in the GS7 strain and the control group. The final fish body weight (FBW) of Ctrl-2 (IBW 300–399 g) and Ctrl-4 (IBW 500–599 g) was significantly lower than those of the corresponding GS7-2 and GS7-4 (<i>P</i> &lt; 0.05), while the FBW of Ctrl-1 (IBW 200–299 g) and Ctrl-3 (IBW 400–499 g) was much significantly lower than the corresponding GS7-1 and GS7-3 (<i>P</i> &lt; 0.01). The BWG, WGR, and SGR of Ctrl-1 and Ctrl-4 were more significantly lower than those of the corresponding GS7-1 and GS7-4 (<i>P</i> &lt; 0.01), while the BWG, WGR, and SGR of Ctrl-2 and Ctrl-3 were more significantly different from the corresponding GS7-2 and GS7-3 (<i>P</i> &lt; 0.0001). Our results seem to point toward the same conclusion that the GS7 strain is better adapted to high plant protein diets than the unselected commercial large yellow croaker. These results will provide a reference for the low-fishmeal culture industry of large yellow croakers and the selection and breeding of strains tolerant to a high percentage of plant proteins in other marine fishes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"732 - 740"},"PeriodicalIF":2.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action","authors":"Athira P. P.,&nbsp;Anju M. V.,&nbsp;Anooja V. V.,&nbsp;Archana K.,&nbsp;Neelima S.,&nbsp;Muhammed Musthafa S.,&nbsp;I. S. Bright Singh,&nbsp;Rosamma Philip","doi":"10.1007/s10126-024-10338-4","DOIUrl":"10.1007/s10126-024-10338-4","url":null,"abstract":"<div><p>Antimicrobial peptides (AMPs), including beta-defensin from fish, are a crucial class of peptide medicines. The focus of the current study is the molecular and functional attributes of <i>Cm</i>Def, a 63-amino acid beta-defensin AMP from Malabar trevally, <i>Carangoides malabaricus</i>. This peptide demonstrated typical characteristics of AMPs, including hydrophobicity, amphipathic nature, and +2.8 net charge. The <i>Cm</i>Def was recombinantly expressed and the recombinant peptide, r<i>Cm</i>Def displayed a strong antimicrobial activity against bacterial fish pathogens with an MIC of 8 µM for <i>V. proteolyticus</i> and 32 µM for <i>A. hydrophila</i>. The <i>E. tarda</i> and <i>V. harveyi</i> showed an inhibition of 94% and 54%, respectively, at 32 µM concentration. No activity was observed against <i>V. fluvialis</i> and <i>V. alginolyticus.</i> The r<i>Cm</i>Def has a multimode of action that exerts an antibacterial effect by membrane depolarization followed by membrane permeabilization and ROS production. r<i>Cm</i>Def also exhibited anti-cancer activities in silico without causing hemolysis. The peptide demonstrated stability under various conditions, including different pH levels, temperatures, salts, and metal ions (KCl and CaCl₂), and remained stable in the presence of proteases such as trypsin and proteinase K at concentrations up to 0.2 µg/100 µl. The strong antibacterial efficacy and non-cytotoxic nature suggest that r<i>Cm</i>Def is a single-edged sword that can contribute significantly to aquaculture disease management.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"696 - 715"},"PeriodicalIF":2.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and Transcriptomic Insights into the Ovary Development of Hemibarbus labeo Injected with Spawn-Inducing Hormones","authors":"Xinming Gao,&nbsp;Yaoping Lv,&nbsp;Qingmin Dai,&nbsp;Ling Zhu,&nbsp;Siqi Liu,&nbsp;Zehui Hu,&nbsp;Junkai Lu,&nbsp;Haidong Zhou,&nbsp;Zufei Mei","doi":"10.1007/s10126-024-10335-7","DOIUrl":"10.1007/s10126-024-10335-7","url":null,"abstract":"<div><p>Naturally, the ovaries of many farmed fish can only develop to stage IV (mainly including stage IV oocytes, known as full-grown postvitellogenic oocytes). Therefore, spawn-inducing hormone injections are used to promote ovary development and oocyte maturation, facilitating reproduction in the aquaculture industry. The study of spawn-inducing hormones and their underlying neuroendocrine mechanisms has been a recent focus in fish reproductive biology. However, the intra-ovarian regulatory mechanisms of ovary development and oocyte maturation after hormone injection require further investigation. In this study, we explored the histological and transcriptomic map of the ovary of <i>Hemibarbus labeo</i> after hormone injection to reveal changes in the ovary. The gonad index significantly increased after hormone injection for 5.5 h, after which no significant change was observed. Histological analysis showed that the nuclei had moved to one side of the oocytes at 5.5 h after hormone injection. Moreover, the volume of the oocytes increased and their yolk membranes thickened. Oocytes then underwent their first meiotic division at 5.5–11 h after hormone injection. Subsequently, the follicular membrane was ruptured, and ovulation was completed at 11–16.5 h after hormone injection. In addition, we identified 3189 differentially expressed genes (DEGs) on comparing the transcriptomes at different time points after hormone injection. These DEGs were significantly enriched in the GO terms of nervous system process, molecular transducer activity, and extracellular region, and the KEGG pathways of TNF signaling and cytokine–cytokine receptor interaction; these may play important roles in ovary development and oocyte maturation. Within these pathways, genes such as <i>apoe</i>, <i>creb3</i>, <i>jun</i>, <i>junb</i>, <i>il11</i>, and <i>il8</i> may play important roles in steroid hormone synthesis and ovulation. Conclusively, our results show detailed sequential dynamics of oocyte development and provide new insights into the intra-ovarian regulatory mechanisms of ovarian development and oocyte maturation in <i>H. labeo</i>. These findings may be important for research on improving egg quality and reproduction in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"672 - 686"},"PeriodicalIF":2.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141441929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HcN57, A Novel Unusual Acidic Silk-Like Matrix Protein from Hyriopsis cumingii, Participates in Framework Construction and Nacre Nucleation During Nacreous Layer Formation","authors":"Can Jin,&nbsp;Fangmengjie Wei,&nbsp;Jiayi Zhang,&nbsp;Xiaoyang Tan,&nbsp;Taixia Fan,&nbsp;Wen Luo,&nbsp;Jiale Li","doi":"10.1007/s10126-024-10339-3","DOIUrl":"10.1007/s10126-024-10339-3","url":null,"abstract":"<div><p>In the classic molecular model of nacreous layer formation, unusual acidic matrix proteins rich in aspartic acid (Asp) residues are essential for nacre nucleation due to their great affinity for binding calcium. However, the acidic matrix proteins discovered in the nacreous layer so far have been weakly acidic with a high proportion of glutamate. In the present study, several silk-like matrix proteins, including the novel matrix protein HcN57, were identified in the ethylenediaminetetraacetic acid-soluble extracts of the nacreous layer of <i>Hyriopsis cumingii</i>. HcN57 is a highly repetitive protein that consists of a high proportion of alanine (Ala, 34.4%), glycine (Gly, 22.5%), and serine (Ser, 11.4%). It forms poly Ala blocks, Gly_nX repeats, an Ala-Gly repeat, and a Ser-Ala-rich region, exhibiting significant similarity to silk proteins found in spider species. The expression of HcN57 was specifically located in the dorsal epithelial cells of the mantle pallium and mantle center. Notably, expression of HcN57 was relatively high during nacreous layer regeneration and pearl nacre deposition, suggesting HcN57 is a silk matrix protein in the nacreous layer. Importantly, HcN57 also contains a certain content of Asp residues, making it an unusual acidic matrix protein present in the nacreous layer. These Asp residues are mainly distributed in three large hydrophilic acidic regions, which showed inhibitory activity against aragonite deposition and morphological regulation of calcite in vitro. Moreover, HcN57-dsRNA injection resulted in failure of nacre nucleation in vivo. Taken together, our results show that HcN57 is a bifunctional silk protein with poly Ala blocks and Gly-rich regions that serve as space fillers within the chitinous framework to prevent crystallization at unnecessary nucleation sites and Asp-rich regions that create a calcium ion supersaturated microenvironment for nucleation in the center of nacre tablets. These observations contribute to a better understanding of the mechanism by which silk proteins regulate framework construction and nacre nucleation during nacreous layer formation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"716 - 731"},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Responses and Larval-Stage Growth of Protandrous Yellowfin Seabream (Acanthopagrus Latus) to Different Polyethylene Microplastics Exposure","authors":"Meng Xu,&nbsp;Wenyu Fang,&nbsp;Genmei Lin,&nbsp;Xiaoshan Zhu,&nbsp;Jianguo Lu","doi":"10.1007/s10126-024-10334-8","DOIUrl":"10.1007/s10126-024-10334-8","url":null,"abstract":"<div><p>Polyethylene microplastics (PE-MPs) were widespread in the marine environment; thus, their influences on marine hermaphroditic fish cannot be ignored. This study intends to evaluate the adverse biological effects of two different sources of PE, identified by Raman spectroscopy, on protandrous yellowfin seabream (<i>Acanthopagrus latus</i>) larvae. Growth retardation, brain lesions, head/body length ratio increase, and neuroendocrine system disorders were found, and growth and neuroendocrine regulation-related genes such as <i>sstr2</i>, <i>ghrb</i>, <i>irs1</i>, UGT2B15, UGT2C1, <i>drd4a</i>, <i>esr2b, hsd3b7</i>, and <i>hsd17b2</i> were identified. PE microbeads (100 μm) showed more severe tissue damage on fish, while environmental PE fibers (500–2500 μm) showed more imperceptible adverse effects. There were 218 DEGs up-regulated and 147 DEGs down-regulated in the environmental PE group, while 1284 (up) and 1267 (down) DEGs were identified in the virgin PE group. PE-MP stress influenced physiological processes like growth and neuroendocrine regulation and cholesterol-steroid metabolism, and caused tissue damage in the fish larvae. The study highlights the effects of environmental PE exposure on hermaphroditic protandrous fish.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"931 - 942"},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Localization of Health-Promoting Peptides Derived from Fish Protein Hydrolyzates on Fish Muscle Proteins","authors":"Shugo Watabe,&nbsp;Nanami Mizusawa,&nbsp;Kenta Hosaka,&nbsp;Shoichiro Ishizaki,&nbsp;Lu Peng,&nbsp;Koji Nagata,&nbsp;Nobuhiko Ueki","doi":"10.1007/s10126-024-10331-x","DOIUrl":"10.1007/s10126-024-10331-x","url":null,"abstract":"<div><p>The four previously reported health-promoting dipeptides, valine-tyrosine, lysine-tryptophan, methionine-phenylalanine, and arginine-isoleucine, found in the fish muscle hydrolyzates, were mainly located in the myosin subfragment-1 heavy chain, whereas the health-promoting tripeptide, alanine-lysine-lysine, was found in the fibrous rod consisting of the myosin subfragment-2 and light meromyosin with a regular coiled-coil structure of α-helix, irrespective of the fish species. Furthermore, the localization of these peptides either in the random coil, β-sheet, or α-helix was also examined in the three-dimensional image, showing no specific tendency. Surprisingly, the same trend was observed even for the mammalian rabbit fast muscle myosin heavy chain. Since a trade-off between myofibrillar ATPase and structural stability has been reported for fish living at low environmental temperatures, it is speculated that fish muscle proteins, when ingested, are easily digested by various proteases in the human digestive tract and provide various health-promoting peptides also in vivo. While fish actin contained only two dipeptides, methionine-phenylalanine and valine-tyrosine, glyceraldehyde 3-phosphate dehydrogenase, one of the major components of fish muscle water-soluble protein, contained all of the four dipeptides and one tripeptide mentioned above.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"843 - 852"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}