锌吸收转运体 ZIP1-II 参与了太平洋牡蛎肝胰腺中的锌积累过程

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ning Kong, Cong Luo, Mengjia Wang, Junyan Zhao, Xiang Li, Lingling Wang, Linsheng Song
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引用次数: 0

摘要

众所周知,太平洋牡蛎(Crassostrea gigas)具有特殊的锌积累能力,这赋予了它对病原体的强大抵抗力,并使其成为膳食锌的绝佳来源。在其他物种中,ZIP1 已被确认为一种重要的锌吸收蛋白,但其在牡蛎中的作用仍不清楚。本研究在千岛湖牡蛎中发现了一个 ZIP1 同源物(CgZIP1-II),属于 Zrt/Irt-like 蛋白(ZIP)家族。CgZIP1-II的mRNA转录本在千层鱼的受检组织中呈组成型表达,在肝胰腺和鳃中的表达水平较高。免疫细胞化学分析发现,CgZIP1-II 蛋白主要位于牡蛎血细胞的质膜上。与对照细胞相比,在转染的 HEK293 细胞中过表达 CgZIP1-II 蛋白会使牡蛎血球细胞中的 CgZIP1-II 蛋白的表达量增加 2.44 倍(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Zinc Uptake Transporter ZIP1-II Is Involved in Zinc Accumulation in the Hepatopancreas of Pacific Oyster Crassostrea gigas

A Zinc Uptake Transporter ZIP1-II Is Involved in Zinc Accumulation in the Hepatopancreas of Pacific Oyster Crassostrea gigas

The Pacific oyster Crassostrea gigas is known to have an exceptional ability to accumulate zinc, which endows it with robust resistance to pathogens and makes it an excellent source of dietary zinc. ZIP1 has been identified as an important zinc uptake protein in other species, but its role in oysters remains unclear. In the present study, a ZIP1 homologue (CgZIP1-II) of the Zrt/Irt-like protein (ZIP) family was identified in C. gigas. The mRNA transcripts of CgZIP1-II were constitutively expressed in examined tissues of C. gigas, with higher levels in the hepatopancreas and gill. After zinc exposure, the mRNA transcripts of CgZIP1-II in the hepatopancreas showed a significant decline from 12 h to 14 d, while those in the gill significantly decreased at 72 h, followed by a recovery to basal level at 7 to 14 d. Immunocytochemical analysis revealed that the CgZIP1-II protein was mainly located at the plasma membrane of oyster hemocytes. Compared to the control cells, overexpression of CgZIP1-II in the transfected HEK293 cells resulted in a 2.44-fold (p < 0.05) increase in zinc content after incubation with 100 μM zinc for 24 h. Inhibition of endogenous CgZIP1-II expression with siRNAs led to a 42% reduction in zinc content in the hepatopancreas of oysters. Similarly, in vivo blocking of CgZIP1-II with anti-CgZIP1-II antibody caused a 43% decrease in zinc content in the hepatopancreas. These results collectively indicated that CgZIP1-II functioned as a zinc uptake transporter in C. gigas and played a certain role in zinc accumulation.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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