Marine Biotechnology最新文献

{"title":"Unveiling the Hidden Biotechnological Potential of the Vermetid Gastropod Vermetus triquetrus: Insights into an Unexplored Marine Resource","authors":"A. Breves,&nbsp;C. Cardoso,&nbsp;C. Afonso,&nbsp;J. Matos,&nbsp;J. Lobo-Arteaga,&nbsp;C. Bartilotti,&nbsp;S. Sales,&nbsp;S. Pedro,&nbsp;N. M. Bandarra","doi":"10.1007/s10126-026-10632-3","DOIUrl":"10.1007/s10126-026-10632-3","url":null,"abstract":"<div><p>The vermetid <i>Vermetus triquetrus</i>, commonly found on intertidal hard substrates in the Mediterranean Sea and the Portuguese coast, remains an underexplored marine resource. This study aimed to determine the total polyphenol content, fatty acid (FA) composition, and biological activity (antioxidant and anti-inflammatory properties) in the visceral mass and the head-foot part of this sessile gastropod from a population collected in Mira Estuary, mainland Portugal. The FA profile of the visceral mass albeit rich in saturated FA had significant levels of long-chain polyunsaturated FA, arachidonic acid (20:4 ω6), 6.3%, and eicosapentaenoic acid (20:5 ω3, EPA), 4.2% of total FA, and a non-negligible content of docosapentaenoic (22:5 ω3), 2.1%. Moreover, though the FA profile of the head-foot part was not attained, regarding other parameters, there were differences between the visceral mass and head-foot part of V. triquetrus. In fact, concerning polyphenol content, whereas the head-foot contained a total of 142 ± 14 mg GAE/100 g dw, the visceral mass reached a high level of 314 ± 90 mg GAE/100 g dw. The antioxidant activity as measured by FRAP showed a similar pattern of higher values in the visceral mass than in the head-foot, 55.6 ± 18.3 µmol Fe2+/g dw vs. 9.8 ± 0.8 µmol Fe2+/g dw. Finally, the ABTS methodology largely agreed with polyphenol levels and FRAP, being the head-foot with 1189 ± 223 µmol Trolox Eq/100 g dw less antioxidant than the visceral mass, 5511 ± 2304 µmol Trolox Eq/100 g dw. Given the presence of anti-inflammatory compounds, such as polyphenols (more abundant in the visceral mass), anti-inflammatory activity, as measured by COX-2 inhibition %, was detected, reaching more than 30% in the visceral mass. Therefore, the gastropod <i>V</i>. <i>triquetrus</i> can be a source of polyphenolic compounds, being specially its visceral mass, not only a rich source of polyphenols, but also a starting material for highly antioxidant extracts. Given the known biological activity of polyphenols and the convergence of results with the FRAP and ABTS antioxidant activity methods, further study on the specific phenolic composition and extract fractionation and refining is warranted.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13216087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics and Proteomics Reveals the Glycogen and Lipid Metabolism Regulating Glycogen Level in Mantle of Jinjiang Oyster (Crassostrea ariakensis)","authors":"Shuqi Zhao,&nbsp;Shengqiang Li,&nbsp;Changfeng Chi,&nbsp;Zhihong Liu,&nbsp;Zhuanzhuan Li,&nbsp;Peizhen Ma,&nbsp;Xiujun Sun,&nbsp;Liqing Zhou,&nbsp;Biao Wu","doi":"10.1007/s10126-026-10639-w","DOIUrl":"10.1007/s10126-026-10639-w","url":null,"abstract":"<div><p>Glycogen is not only a critical energy storage molecule in oyster but also a key flavor-determining factor that affect their fatness, with glycogen content directly governing oyster quality. However, the molecular mechanisms underlying glycogen storage and metabolism in shellfish (including oyster) remain incompletely understood. In order to clarify the regulatory mechanisms of glycogen metabolism in Jinjiang oyster (<i>C. ariakensis)</i>, we performed integrated transcriptomic and proteomic analyses on the mantle tissues with high glycogen content (HG) and low glycogen content (LG). The results identified 899 differentially expressed genes (DEGs) at the transcriptional level and 1294 differentially expressed proteins (DEPs) at the translational level, respectively. Functional enrichment analysis demonstrated that these DEGs and DEPs were mainly enriched in glycogen metabolism and energy metabolism-related pathways, including the insulin signaling pathway, starch and sucrose metabolism, and glycerolipid metabolism. In the HG, numerous key genes and proteins were significantly involved in glycogen synthesis, gluconeogenesis, glycolysis pathways and fatty acid metabolism were significantly upregulated. These findings indicate these multiple metabolic processes co-regulate the level of glycogen in the mantle, providing a theoretical basis for elucidating molecular mechanisms of glycogen metabolism and valuable insights for the breeding of high-glycogen oyster varieties.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148025547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ulvan from Ulva lactuca Promotes Keratinocyte Migration, Adhesion, and Photoprotection Against UVB Damage","authors":"Aida Lahmar,&nbsp;Abir Salek,&nbsp;Imen Waer,&nbsp;Haifa Messai,&nbsp;Ayda Baffoun,&nbsp;Leila Chekir Ghedira","doi":"10.1007/s10126-026-10633-2","DOIUrl":"10.1007/s10126-026-10633-2","url":null,"abstract":"<div><p>Ulvan, a sulfated polysaccharide extracted from the sea lettuce, is gaining wider appreciation for its health-promoting and medicinal qualities. In the current study, <i>Ulva lactuca</i> was collected from the coastal site of Monastir, Tunisia. Ulvan was extracted and characterized using Fourier-transform infrared (FTIR) spectroscopy, confirming the presence of characteristic sulfate and carboxyl groups. The antiradical, antioxidant activity, and carbohydrate content were also estimated. The photoprotective effects of ulvan were evaluated in human keratinocyte (HaCaT) cells exposed to UVB radiation. Pre-treatment with ulvan (25 and 50 µg/mL) significantly enhanced cell viability, reduced reactive oxygen species (ROS) production, preserved lysosomal function, and inhibited apoptosis. Additionally, ulvan enhanced cell migration and enhanced adhesion on extracellular matrix proteins, including laminin, collagen I, poly-L-lysine, vitronectin, and fibrinogen, demonstrating its role in maintaining cellular integrity under UVB stress. An enriched-ulvan emulsion was formulated. SPF evaluation suggested limited sunscreen effectiveness. Therefore, the photoprotective effects are mediated via antioxidant and cytoprotective mechanisms. This result support its potential as a natural marine-derived ingredient for functional cosmetic and dermatological products.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147985976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Proteomic Profiling of Pinctada fucata Shell Nacre Defines a Solubility-Based Type Classification of Shell Matrix Proteins","authors":"Keisuke Oshima,&nbsp;Lumi Negishi,&nbsp;Takashi Atsumi,&nbsp;Hitoshi Kurumizaka,&nbsp;Michio Suzuki","doi":"10.1007/s10126-026-10631-4","DOIUrl":"10.1007/s10126-026-10631-4","url":null,"abstract":"<div><p>Shell matrix proteins (SMPs) are key organic components of molluscan biominerals, yet previous nacre proteomics have remained largely qualitative, limiting evaluation of the abundance and fraction association of individual SMPs. Here, we established a quantitative proteomic approach for the nacreous layer of the pearl oyster <i>Pinctada fucata</i> by integrating optimized shell preservation, stepwise fractionation, and data-independent acquisition (DIA) proteomics. SMPs were separated into an ethylenediaminetetraacetic acid (EDTA)-soluble matrix (ESM), an EDTA-insoluble but sodium dodecyl sulfate/dithiothreitol (SDS/DTT)-soluble matrix (SSM), and an SDS/DTT-insoluble matrix (ISM). DIA outperformed data-dependent acquisition in proteome coverage and enabled quantification of 327 SMPs across a broad dynamic range. Fraction-resolved abundance profiling showed that each fraction was characterized by distinct SMP compositions. To summarize these distributions, we introduced a solubility-based type classification that grouped SMPs into four types according to their quantitative partitioning among fractions. Well-known SMPs, including nacrein, Pif 80, and MSI60, were assigned to intuitively consistent types, whereas proteases, protease inhibitors, and tyrosinases also showed biased type distributions. These results support a three-compartment model of the nacreous layer consisting of (i) an insoluble interlamellar membrane core, (ii) a relatively extractable interfacial layer, and (iii) a soluble matrix fraction enriched in proteins potentially involved in ionic regulation and protein maturation. This study provides a quantitative framework for understanding coordinated SMP functions during nacre formation and for comparative analyses of molluscan shell proteomes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13190495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Extracts from Yucatán Marine Invertebrates as Modulators of Microalgal Dynamics","authors":"Dawrin Pech-Puch,&nbsp;Rita Pires,&nbsp;Bárbara A. Rebelo,&nbsp;Diana Grilo,&nbsp;Carlos Gonzáles-Salas,&nbsp;Susana Eunice Calva-Pérez,&nbsp;Susana M. Gomes,&nbsp;Harold Villegas-Hernández,&nbsp;Sergio Guillén-Hernández,&nbsp;Abel M. Forero,&nbsp;Jaime Rodriguez,&nbsp;Carlos Jiménez,&nbsp;Oscar A. Lenis-Rojas,&nbsp;Rita Abranches","doi":"10.1007/s10126-026-10630-5","DOIUrl":"10.1007/s10126-026-10630-5","url":null,"abstract":"<div><p>The marine environment is a rich source of bioactive compounds with a wide range of applications. In this study, 65 extracts from sponges, ascidians, and a gorgonian collected from the Yucatán Peninsula, Mexico, were evaluated for their modulatory effects on the growth of four microalgal species. Some extracts were found to exhibit pronounced algicidal activity, while others promoted growth. Bioassay-guided fractionation of the extract from <i>Haliclona (Rhizoniera) curacaoensis</i> led to the identification of arenosclerins A and C as potent algicidal agents, active across all tested microalgae. In contrast, the extract from <i>Halichondria melanadocia</i> enhanced microalgal growth, and chemical analysis revealed the presence of medelamine A and B as putative growth-promoting compounds. This study provides a new report on microalgae-modulating activities of marine sponge metabolites. The dual activity observed, both inhibitory and stimulatory, not only contributes to our understanding of marine compound diversity but also underscores the potential of these marine-derived compounds for sustainable environmental management practices.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Marine Polysaccharides from Uroteuthis duvauceli Gladius Waste: Structural Insights and Biofunctional Properties","authors":"Naveena Tamilarasu,&nbsp;Annathai Pitchai,&nbsp;Pasiyappazham Ramasamy","doi":"10.1007/s10126-026-10635-0","DOIUrl":"10.1007/s10126-026-10635-0","url":null,"abstract":"<div><p>Marine ecosystems represent a significant source of bioactive compounds, with cephalopods such as <i>Uroteuthis duvauceli demonstrating considerable</i> therapeutic potential. In this study, marine polysaccharides were extracted from the gladius of <i>U. duvauceli and</i> characterized using Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), and X-Ray Diffraction (XRD). The polysaccharide demonstrated notable antioxidant activity, including a DPPH scavenging effect of 45.17% at 10 mg/ml, superoxide radical scavenging activity of 64.19% at 10 mg/ml, and chelating ability of 71.59% at 10 mg/ml. Furthermore, the polysaccharide exhibited antibacterial activity against <i>Escherichia coli</i> (15 ± 1.53 mm) and <i>Staphylococcus aureus</i> (10 ± 0.58 mm), as determined by agar diffusion assays. Anticoagulant efficacy was assessed using Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) assays, yielding an APTT of 112 ± 3.52 s and a PT of 74 ± 2.25 s. These results indicate that <i>U. duvauceli</i> polysaccharides possess significant antibacterial, antioxidant, and anticoagulant properties, underscoring their potential as natural bioactive agents for pharmaceutical development. Further research is required to clarify their mechanisms of action and assess in vivo efficacy.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bath-Immersion Delivery of nanos-MO-Vivo During Embryogenesis Perturbs Gonadal Development in the Pacific Oyster (Crassostrea gigas)","authors":"Huihui Wang,&nbsp;Hongli Li,&nbsp;Xin Tao,&nbsp;Hong Yu,&nbsp;Qi Li","doi":"10.1007/s10126-026-10641-2","DOIUrl":"10.1007/s10126-026-10641-2","url":null,"abstract":"<div><p>The regulation of germline-associated processes during early embryogenesis is an important aspect of gonadal development in aquaculture species. Here, we employed bath-immersion delivery of a <i>nanos</i>-targeting Morpholino-Vivo (<i>nanos</i>-MO-Vivo) during embryogenesis to transiently disrupt early germline development in the Pacific oyster <i>Crassostrea gigas</i>. Post-fertilization immersion with 100 µM <i>nanos</i>-MO-Vivo for 6 h achieved effective repression of germline markers while maintaining acceptable embryo viability. Transcriptomic analyses at the blastula stage revealed coordinated downregulation of PGC markers (<i>vasa</i>, <i>Piwil1</i>) and germline-associated regulatory pathways. Long-term rearing revealed that transient embryonic perturbation of <i>nanos</i> resulted in persistent impairment of adult gonadal development, characterized by reduced gonadal coverage and diminished gametogenic tissue. In silico analysis based on single-cell transcriptomic data further supported <i>nanos</i>-associated regulatory effects within the PGC population. High sequence conservation of nanos among <i>Crassostrea</i> species was accompanied by comparable knockdown responses in <i>C. angulata</i> and <i>C. nippona</i>, suggesting the observed effects are not restricted to a single oyster species. This study links transient embryonic perturbation of germline-associated regulation to long-term gonadal phenotypes, providing insight into reproductive modulation in molluscan aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148100830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic Effects of Cnidarian Venoms: One Cancer, Two Cell Lines","authors":"Khouloud Azaiez,&nbsp;Nurçin Killi,&nbsp;Kemal Emir Türk,&nbsp;Hazal Kalsın Demir,&nbsp;Anara Babayeva,&nbsp;Esin Sakallı Çetin","doi":"10.1007/s10126-026-10625-2","DOIUrl":"10.1007/s10126-026-10625-2","url":null,"abstract":"<div><p>This study aimed to evaluate the cytotoxic effects of jellyfish venoms on two different colon cancer cell lines and to compare their activity with that observed in a healthy colon cell line used as a control. Venoms were isolated from specimens of <i>Aurelia aurita</i>, <i>Rhopilema nomadica</i>, and <i>Anemonia sulcata</i> collected in their natural habitats. These venoms were applied to HT29 and Caco2 colon cancer cell lines, as well as to the CCD18co healthy colon cell line cultured in vitro. The IC₅₀ values of the venoms were determined using the MTT assay. Given the IC₅₀ results, <i>A. aurita</i> displayed the highest cytotoxic potency, <i>R. nomadica</i> showed a moderate and cell line–dependent effect, and <i>A. sulcata</i> exhibited a delayed, high-dose cytotoxicity profile, becoming clearly evident at 72 h. Based on these results, Annexin-V, ROS, and MMP analyses were performed to investigate the mechanisms of cell death. Our findings showed that <i>A. aurita</i> venom was more effective on HT29 cells after 48 h and caused minimal damage to CCD18co healthy cells. <i>A. sulcata</i> venom exhibited cytotoxic effects on HT29 cells at both 48 and 72 h, while exerting limited toxicity on healthy CCD18co cells. <i>R. nomadica</i> venom showed cytotoxicity on Caco2 cells at 48 h. Among the three venoms, <i>R. nomadica</i> exhibited the highest cytotoxic effect on CCD18co healthy colon cells. In conclusion, venoms from all three Cnidarian species demonstrated cytotoxic effects on two different colon cancer cell lines. The cytotoxicity was found to be apoptosis-related. Moreover, the venoms showed selective activity across different cancer cell lines.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-026-10625-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Fungi Associated with Seaweeds as Potential Producers of Antimicrobial Compounds","authors":"Maria da Luz Calado,&nbsp;Débora Santos,&nbsp;Patrícia Susano,&nbsp;Susete Pintéus,&nbsp;Alice Martins,&nbsp;Joana Silva,&nbsp;Miguel A. M. Oliveira,&nbsp;Celso Alves,&nbsp;Rui Pedrosa,&nbsp;Patrick G. Murray,&nbsp;Katie Shiels,&nbsp;Maria Jorge Campos","doi":"10.1007/s10126-026-10626-1","DOIUrl":"10.1007/s10126-026-10626-1","url":null,"abstract":"<div><p>The urgent need for new molecules capable of targeting multidrug-resistant microorganisms has redirected bioprospecting efforts toward less explored marine environments and their associated communities, such as marine mycobiota. This study aimed to evaluate the antimicrobial potential of endophytic fungi isolated from various seaweed species and infer the mechanisms of action and chemical profile of most potent fungal extracts. Simultaneously, it intended to contribute to a better understanding of ecological requirements of each fungal species. Thirty-one ascomycetous fungi were recovered from the seaweed hosts, with a higher representation of the genera <i>Aspergillus</i> and <i>Penicillium</i>. Only two fungal species revealed notable antimicrobial abilities: <i>Penicillium rubens</i> synthesised extracts with pronounced antibacterial effects against <i>Escherichia coli</i>,<i> Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>, while extracts of <i>Penicillium brevicompactum</i> inhibited the growth of <i>Candida albicans</i>. Although similar activities have been described in a few previous studies, this is the first report of a markedly inhibitory effect against a pathogenic yeast demonstrated by <i>P. brevicompactum.</i> Moreover, the mechanisms of action underlying these activities were explored for the first time in this study. Specifically, the antimicrobial activities demonstrated by fungal extracts were likely mediated by membrane potential imbalance and DNA damage, associated with the presence of L-norvaline and formylphosphonate. Despite the overall consistency of the results, slight to pronounced differences were also observed in the antibacterial activities and mechanisms of action of the extracts from <i>P. rubens</i> strains. These findings support the hypothesis that marine fungal species possess, and express distinct metabolic profiles shaped by micro- and/or macro-environmental factors.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-026-10626-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytohormone-Assisted Bioprocess Engineering for Enhanced Omega Fatty Acid Production in Marine Thraustochytrids Under Cold Stress Conditions","authors":"Ajeet Singh Chauhan,&nbsp;Reeta Rani Singhania,&nbsp;Jo-Shu Chang,&nbsp;Grace Sathyanesan Anisha,&nbsp;Cheng-Di Dong,&nbsp;Anil Kumar Patel","doi":"10.1007/s10126-026-10627-0","DOIUrl":"10.1007/s10126-026-10627-0","url":null,"abstract":"<div><p>Microbial platforms are now recognized as sustainable sources of long-chain polyunsaturated fatty acids (PUFAs). This study presents a novel bioprocessing approach that integrates abiotic stress with exogenous phytohormones, indole-3-acetic acid (IAA), salicylic acid (SA), and abscisic acid (ABA) to enhance the biosynthesis of PUFAs in marine <i>Thraustochytrium</i> sp. BM2. While individual stress strategies are known to either enhance lipid accumulation or modulate oxidative responses. The combined effect of phytohormones and multiple treatments of cold stress conditions (4 °C) enhanced the PUFAs fraction (including DHA, EPA, DPA, and ARA) and lowered the SFAs fraction. IAA combined with cold stress marginally enhanced lipid yield by 73.3% (up to 9.48 g L⁻¹), but the composition of fatty acids was significantly changed. These changes were aligned with significant increases in EPA (100%, 0.38 g L⁻¹), DPA (19%, 1.06 g L⁻¹), and DHA (19%, 1.94 g L⁻¹). SA in combination with cold stress achieved a 39.7% increase in lipid yield (7.63 g L⁻¹), with corresponding improvements in EPA (21%, 0.23 g L⁻¹), DHA (5.2%, 1.72 g L⁻¹), while ARA (300%, 0.12 g L⁻¹). The combined effect of ABA resulted in enhanced levels of EPA, DPA, DHA, and ARA by 21%, 39%, 16%, and 200%, respectively. These results were statistically validated by ANOVA, with all variations demonstrating significance at <i>p</i> &lt; 0.05. This sustainable and eco-efficient strategy addresses the limitations of fish oil-based PUFA production, aligning with the United Nations Sustainable Development Goals (SDGs: 7, 12, and 13) and offering a scalable alternative for the nutraceutical and biofuel industries.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}