Marine Biotechnology最新文献

{"title":"A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp.","authors":"José Luis Rodríguez-Mejía,&nbsp;Itzel Anahí Hidalgo-Manzano,&nbsp;Luis Felipe Muriel-Millán,&nbsp;Nancy Rivera-Gomez,&nbsp;Diana X. Sahonero-Canavesi,&nbsp;Edmundo Castillo,&nbsp;Liliana Pardo-López","doi":"10.1007/s10126-024-10308-w","DOIUrl":"10.1007/s10126-024-10308-w","url":null,"abstract":"<div><p>Marine environments harbor a plethora of microorganisms that represent a valuable source of new biomolecules of biotechnological interest. In particular, enzymes from marine bacteria exhibit unique properties due to their high catalytic activity under various stressful and fluctuating conditions, such as temperature, pH, and salinity, fluctuations which are common during several industrial processes. In this study, we report a new esterase (EstGoM) from a marine <i>Pseudomonas</i> sp. isolated at a depth of 1000 m in the Gulf of Mexico. Bioinformatic analyses revealed that EstGoM is an autotransporter esterase (type Va) and belongs to the lipolytic family II, forming a new subgroup. The purified recombinant EstGoM, with a molecular mass of 67.4 kDa, showed the highest hydrolytic activity with <i>p</i>-nitrophenyl octanoate (<i>p</i>-NP C8), although it was also active against <i>p</i>-NP C4, C5, C10, and C12. The optimum pH and temperature for EstGoM were 9 and 60 °C, respectively, but it retained more than 50% of its activity over the pH range of 7–11 and temperature range of 10–75 °C. In addition, EstGoM was tolerant of up to 1 M NaCl and resistant to the presence of several metal ions, detergents, and chemical reagents, such as EDTA and β-mercaptoethanol. The enzymatic properties of EstGoM make it a potential candidate for several industrial applications.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"447 - 459"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10308-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140837883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Muscle Growth and Transcription of a Mutant MSTN Gene in Olive Flounder (Paralichthys olivaceus)","authors":"Ju-Won Kim,&nbsp;Julan Kim,&nbsp;Ja Young Cho,&nbsp;Younhee Shin,&nbsp;Hyojung Son,&nbsp;Subramaniyam Sathiyamoorthy,&nbsp;Bo-Seong Kim,&nbsp;Young-Ok Kim,&nbsp;Byeong-chul Kang,&nbsp;Hee Jeong Kong","doi":"10.1007/s10126-024-10322-y","DOIUrl":"10.1007/s10126-024-10322-y","url":null,"abstract":"<div><p>Myostatin (MSTN, also known as growth differentiation factor-8 (GDF-8)), a member of the transforming growth factor β (TGF-β) superfamily, functions as a negative regulator of skeletal muscle development and growth. However, it is also expressed in a wide range of tissues in fish and thus may have more diverse roles in this group than in mammals. In this study, we assessed the genome-wide transcriptional expression pattern associated with the CRISPR/Cas9-mutated MSTN gene in the olive flounder (<i>Paralichthys olivaceus</i>) in association with changes in cell proliferation and transportation processes. There were no differences in the hepatosomatic index, and the growth of male and female fish increased in the F1 progeny of the MSTN mutants. Furthermore, the histopathological analysis showed that myostatin editing resulted in a 41.24% increase in back muscle growth and 46.92% increase in belly muscle growth in male flounder compared with normal flounder, and a 16.01% increase in back muscle growth and 14.26% increase in belly muscle growth in female flounder compared with normal flounder. This study demonstrates that editing of the myostatin gene enhances muscle growth in olive flounder, with a notably more pronounced effect observed in males. Consequently, myostatin-edited male flounder could represent a valuable asset for the flounder aquaculture industry.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"599 - 608"},"PeriodicalIF":2.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140837596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hsp70 Knockdown in the Brine Shrimp Artemia franciscana: Implication on Reproduction, Immune Response and Embryonic Cuticular Structure","authors":"Irene K. R. Tiong,&nbsp;Cher Chien Lau,&nbsp;Patrick Sorgeloos,&nbsp;Mimi Iryani Mat Taib,&nbsp;Tengku Sifzizul Tengku Muhammad,&nbsp;Muhd Danish-Daniel,&nbsp;Min Pau Tan,&nbsp;Liying Sui,&nbsp;Min Wang,&nbsp;Yeong Yik Sung","doi":"10.1007/s10126-024-10318-8","DOIUrl":"10.1007/s10126-024-10318-8","url":null,"abstract":"<div><p>The potential functional role(s) of heat shock protein 70 (Hsp70) in the brine shrimp, <i>Artemia franciscana</i>, a crucial crustacean species for aquaculture and stress response studies, was investigated in this study. Though we have previously reported that Hsp70 knockdown may have little or no impact on <i>Artemia</i> development, the gestational survival and number of offspring released by adult females were impaired by obscuring Hsp70 synthesis. Transcriptomic analysis revealed that several cuticle and chitin synthetic genes were downregulated, and carbohydrate metabolic genes were differentially expressed in Hsp70-knockdown individuals. A more comprehensive microscopic examination performed in this study revealed exoskeleton structural destruction and abnormal eye lenses featured in Hsp70-deficient adult females 48 h after Hsp70 dsRNA injection. Cysts produced by these Hsp70-deficient broods, instead, had a defective shell and were smaller in size, whereas nauplii had shorter first antennae and a rougher body epicuticle surface. Changes in carbohydrate metabolism caused by Hsp70 knockdown affected glycogen levels in adult <i>Artemia</i> females, as well as trehalose in cysts released from these broods, indicating that Hsp70 may play a role in energy storage preservation. Outcomes from this work provided novel insights into the roles of Hsp70 in <i>Artemia</i> reproduction performance, cyst formation, and exoskeleton structure preservation. The findings also support our previous observation that Hsp70 knockdown reduced <i>Artemia</i> nauplius tolerance to bacterial pathogens, which could be explained by the fact that loss of Hsp70 downregulated several Toll receptor genes (<i>NT1</i> and <i>Spaetzle</i>) and reduced the integrity of the exoskeleton, allowing pathogens to enter and cause infection, ultimately resulting in mortality.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"562 - 574"},"PeriodicalIF":2.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140812909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting TtVgR via siRNA Knockdown Elicits Ovarian Cell Death in the Tri-spine Horseshoe Crab","authors":"Kianann Tan,&nbsp;Xiaowan Ma,&nbsp;Boyu Su,&nbsp;Chen Zhan,&nbsp;Xin Yang,&nbsp;Khor Waiho,&nbsp;Leong-Seng Lim,&nbsp;Kit Yue Kwan","doi":"10.1007/s10126-024-10319-7","DOIUrl":"10.1007/s10126-024-10319-7","url":null,"abstract":"<div><p>The vitellogenin present in the bloodstream undergoes internalization into developing oocytes through the vitellogenin receptor (<i>VgR</i>), a process mediated by receptor-mediated endocytosis. <i>VgR</i> plays a crucial role in facilitating the accumulation of vitellogenin and the maturation of oocytes. In this study, we characterized a <i>Tachypleus tridentatus</i> vitellogenin receptor (<i>TtVgR</i>) gene from the tri-spine horseshoe crab, revealing a length of 1956 bp and encoding 652 amino acid residues with 12 exons. <i>TtVgR</i> has a molecular weight of 64.26 kDa and an isoelectric point of 5.95. Predictions indicate 85 phosphorylation sites and 7 glycosylation sites within <i>TtVgR</i>. Transcriptional analysis demonstrated specific expression of <i>TtVgR</i> in the ovary and yellow connective tissue. <i>TtVgR</i> was identified and distributed in the plasma membrane of oocytes. The siRNA-mediated <i>TtVgR</i> knockdown significantly reduced the transcriptional activity of <i>TtVgR</i>. This depletion induced excessive ROS production, resulting in DNA damage in ovarian primary cells. TUNEL and flow cytometry analyses confirmed ovarian cell apoptosis following <i>TtVgR</i> knockdown, indicating DNA damage in ovarian primary cells. These findings underscore the importance of <i>TtVgR</i> in ovarian cell development, suggesting its potential involvement in vitellogenesis and oocyte maturation. This knowledge may inform innovative breeding strategies and contribute to the sustainable management and conservation of the tri-spine horseshoe crab.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"575 - 587"},"PeriodicalIF":2.6,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity","authors":"Xue Li,&nbsp;Miao Yang,&nbsp;Kunlian Mo,&nbsp;Yonghua Hu,&nbsp;Hanjie Gu,&nbsp;Dongmei Sun,&nbsp;Shixiang Bao,&nbsp;Huiqin Huang","doi":"10.1007/s10126-024-10311-1","DOIUrl":"10.1007/s10126-024-10311-1","url":null,"abstract":"<div><p>Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from <i>Sargassum</i> sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as <i>Microbulbifer thermotolerans</i>. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl₃, while exhibiting the best stability at 30 °C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2–DP5 and monosaccharide while reacting for 36 h. The complete genome of <i>M. thermotolerans</i> HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"488 - 499"},"PeriodicalIF":2.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140651962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic Matrices of Calcium Carbonate Biominerals Improve Osteoblastic Mineralization","authors":"Sarah Nahle,&nbsp;Camille Lutet-Toti,&nbsp;Yuto Namikawa,&nbsp;Marie-Hélène Piet,&nbsp;Alice Brion,&nbsp;Sylvie Peyroche,&nbsp;Michio Suzuki,&nbsp;Frédéric Marin,&nbsp;Marthe Rousseau","doi":"10.1007/s10126-024-10316-w","DOIUrl":"10.1007/s10126-024-10316-w","url":null,"abstract":"<div><p>Many organisms incorporate inorganic solids into their tissues to improve functional and mechanical properties. The resulting mineralized tissues are called biominerals. Several studies have shown that nacreous biominerals induce osteoblastic extracellular mineralization. Among them, <i>Pinctada margaritifera</i> is well known for the ability of its organic matrix to stimulate bone cells. In this context, we aimed to study the effects of shell extracts from three other <i>Pinctada</i> species (<i>Pinctada radiata</i>, <i>Pinctada maxima</i>, and <i>Pinctada fucata</i>) on osteoblastic extracellular matrix mineralization, by using an in vitro model of mouse osteoblastic precursor cells (MC3T3-E1). For a better understanding of the <i>Pinctada</i>-bone mineralization relationship, we evaluated the effects of 4 other nacreous mollusks that are phylogenetically distant and distinct from the <i>Pinctada</i> genus. In addition, we tested 12 non-nacreous mollusks and one extra-group. Biomineral shell powders were prepared, and their organic matrix was partially extracted using ethanol. Firstly, the effect of these powders and extracts was assessed on the viability of MC3T3-E1. Our results indicated that neither the powder nor the ethanol-soluble matrix (ESM) affected cell viability at low concentrations. Then, we evaluated osteoblastic mineralization using Alizarin Red staining and we found a prominent MC3T3-E1 mineralization mainly induced by nacreous biominerals, especially those belonging to the <i>Pinctada</i> genus. However, few non-nacreous biominerals were also able to stimulate the extracellular mineralization. Overall, our findings validate the remarkable ability of CaCO₃ biomineral extracts to promote bone mineralization. Nevertheless, further in vitro and in vivo studies are needed to uncover the mechanisms of action of biominerals in bone.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"539 - 549"},"PeriodicalIF":2.6,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140800656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Efficient Vector-Based CRISPR/Cas9 System in Zebrafish Cell Line","authors":"Xiaokang Ye,&nbsp;Jiali Lin,&nbsp;Qiuji Chen,&nbsp;Jiehuan Lv,&nbsp;Chunsheng Liu,&nbsp;Yuping Wang,&nbsp;Shuqi Wang,&nbsp;Xiaobo Wen,&nbsp;Fan Lin","doi":"10.1007/s10126-024-10320-0","DOIUrl":"10.1007/s10126-024-10320-0","url":null,"abstract":"<div><p>The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely applied in animals as an efficient genome editing tool. However, the technique is difficult to implement in fish cell lines partially due to the lack of efficient promoters to drive the expression of both sgRNA and the Cas9 protein within a single vector. In this study, it was indicated that the zebrafish U6 RNA polymerase III (ZFU6) promoter could efficiently induce tyrosinase (<i>tyr</i>) gene editing and lead to loss of retinal pigments when co-injection with Cas9 mRNA in zebrafish embryo. Furthermore, an optimized all-in-one vector for expression of the CRISPR/Cas9 system in the zebrafish fibroblast cell line (PAC2) was constructed by replacing the human U6 promoter with ZFU6 promoter, basing on the lentiCRISPRV2 system that widely applied in mammal cells. This new vector could successfully target the cellular communication network factor 2a (<i>ctgfa</i>) gene and demonstrated its function in the PAC2 cell. Notably, the vector could also be used to edit the endogenous <i>EMX1</i> gene in the mammal 293 T cell line, implying its wide application potential. In conclusion, we established a new gene editing tool for zebrafish cell line, which could be a useful in vitro platform for high-throughput analyzing gene function in fish.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"588 - 598"},"PeriodicalIF":2.6,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Investigation for Early Sex Determination in Alive Adult European Seabass (Dicentrarchus labrax) Using cyp19a1a, dmrt1a, and dmrt1b Genes Expression in Tail Fin tissues","authors":"Samy Y. El-Zaeem,&nbsp;Amr El-Hanafy,&nbsp;Alaa A. El-Dahhar,&nbsp;Ayaat M. Elmaghraby,&nbsp;Sara F. Ghanem,&nbsp;Amany M. Hendy","doi":"10.1007/s10126-024-10313-z","DOIUrl":"10.1007/s10126-024-10313-z","url":null,"abstract":"<div><p>This study is the first investigation for using sex-related gene expression in tail fin tissues of seabass as early sex determination without killing the fish. The European seabass (<i>Dicentrarchus labrax</i>) is gonochoristic and lacks distinguishable sex chromosomes, so, sex determination is referred to molecular actions for some sex-related genes on autosomal chromosomes which are well known such as <i>cyp19a1a</i>, <i>dmrt1a</i>, and <i>dmrt1b</i> genes which play crucial role in gonads development and sex differentiation. <i>cyp19a1a</i> is expressed highly in females for ovarian development and <i>dmrt1a</i> and <i>dmrt1b</i> are for testis development in males. In this study, we evaluated the difference in the gene expression levels of studied genes by qPCR in tail fins and gonads. We then performed discriminant analysis (DA) using morphometric traits and studied gene expression parameters as predictor tools for fish sex. The results revealed that <i>cyp19a1a</i> gene expression was significantly higher in future females’ gonads and tail fins (<i>p</i> ≥ 0.05). Statistically, <i>cyp19a1a</i> gene expression was the best parameter to discriminate sex even the hit rate of any other variable by itself could not correctly classify 100% of the fish sex except when it was used in combination with <i>cyp19a1a</i>. In contrast, <i>Dmrt1a</i> gene expression was higher in males than females but there were difficulties in analyzing <i>dmrt1a</i> and <i>dmrt1b</i> expressions in the tail because levels were low. So, it could be used in future research to differentiate and determine the sex of adult fish using the <i>cyp19a1a</i> gene expression marker without killing or sacrificing fish.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"423 - 431"},"PeriodicalIF":2.6,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10313-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140669309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Poly-β-Hydroxybutyrate Improved the Growth, Non-specific Immunity, Digestive Enzyme Activity, Intestinal Morphology, Phagocytic Activity, and Disease Resistance Against Vibrio parahaemolyticus of Pacific White Shrimp, Penaeus vannamei","authors":"Suhyeok Kim,&nbsp;Jaebeom Shin,&nbsp;Nalin Medagoda,&nbsp;Sera Choi,&nbsp;So Yun Park,&nbsp;Jeung-Yil Park,&nbsp;Kyeong-Jun Lee","doi":"10.1007/s10126-024-10317-9","DOIUrl":"10.1007/s10126-024-10317-9","url":null,"abstract":"<div><p>This study assessed the effects of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, feed efficiency, non-specific immunity, digestive enzyme capacity, phagocytic activity, hemocyte count, intestinal morphology, and disease resistance against <i>Vibrio parahaemolyticus</i> of Pacific white shrimp (<i>Penaeus vannamei</i>). Six diets were prepared by supplementing graded levels of PHB at 0.00, 0.25, 0.50, 1.00, 2.00, and 4.00% (Con, P0.25, P0.5, P1.0, P2.0, and P4.0, respectively). Triplicate groups of 90 shrimps (initial body weight 0.25 ± 0.01 g) per treatment were randomly assigned and fed an experimental diet for 56 days. The growth performance of shrimp was significantly improved by 1% dietary PHB supplementation. PHB-included diets fed shrimp showed significantly improved hepatopancreatic trypsin, chymotrypsin, and pepsin activities. Villus height was significantly increased with dietary PHB supplementation, and villus width was increased at a 1% inclusion level. P0.25, P0.5, and P4.0 groups significantly increased phenoloxidase activity, and the P2.0 group significantly increased anti-protease activity compared to the Con group. The survival of shrimp challenged against <i>V. parahaemolyticus</i> was higher in P0.5, P1.0, and P2.0 groups than in the Con diet. Dietary PHB supplementation improved weight gain, digestive enzyme activity, intestinal morphology, non-specific immunity, and disease resistance against <i>V. parahaemolyticus</i> of shrimp. According to the above observations, the optimal dietary PHB supplementation level for maximum weight gain would be 1% for Pacific white shrimp.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"550 - 561"},"PeriodicalIF":2.6,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Temperature-Induced Differential Expression of miRNA Mediates Liver Inflammatory Response in Tsinling Lenok Trout (Brachymystax lenok tsinlingensis)","authors":"Xiaobin Xie,&nbsp;Yibo Wang,&nbsp;Fang Ma,&nbsp;Ruilin Ma,&nbsp;Leqiang Du,&nbsp;Xin Chen","doi":"10.1007/s10126-024-10315-x","DOIUrl":"10.1007/s10126-024-10315-x","url":null,"abstract":"<div><p>High-temperature stress poses a significant environmental challenge for aquatic organisms, including tsinling lenok trout (<i>Brachymystax lenok tsinlingensis</i>). This study aimed to investigate the role of microRNAs (miRNAs) in inducing liver inflammation in tsinling lenok trout under high-temperature stress. Tsinling lenok trout were exposed to high-temperature conditions (24 °C) for 8 h, and liver samples were collected for analysis. Through small RNA sequencing, we identified differentially expressed miRNAs in the liver of high-temperature-stressed tsinling lenok trout compared to the control group (maintained at 16 °C). Several miRNAs, including novel-m0105-5p and miR-8159-x, showed significant changes in expression levels. Additionally, we conducted bioinformatics analysis to explore the potential target genes of these differentially expressed miRNAs. Our findings revealed that these miRNA target genes are involved in inflammatory response pathways, such as <i>NFKB1</i> and <i>MAP3K5</i>. The downregulation of novel-m0105-5p and miR-8159-x in the liver of high-temperature-stressed tsinling lenok trout suggests their role in regulating liver inflammatory responses. To validate this, we performed a dual-luciferase reporter assay to confirm the regulatory relationship between miRNAs and target genes. Our results demonstrated that novel-m0105-5p and miR-8159-x enhance the inflammatory response of hepatocytes by promoting the expression of <i>NFKB1</i> and <i>MAP3K5</i>, respectively. In conclusion, our study provides evidence that high-temperature stress induces liver inflammation in tsinling lenok trout through dysregulation of miRNAs. Understanding the molecular mechanisms underlying the inflammatory response in tsinling lenok trout under high-temperature stress is crucial for developing strategies to mitigate the negative impacts of environmental stressors on fish health and aquaculture production.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"526 - 538"},"PeriodicalIF":2.6,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140677920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}