Marine Biotechnology最新文献

{"title":"Identification of a Candidate Sex Determination Region and Sex-specific Molecular Markers of Spinibarbus caldwelli","authors":"Maoyuan Wang,&nbsp;Yannian Wu,&nbsp;Yang Li,&nbsp;Dongling Zhang,&nbsp;Mingyong Lai,&nbsp;Xiaojun Ye","doi":"10.1007/s10126-026-10640-3","DOIUrl":"10.1007/s10126-026-10640-3","url":null,"abstract":"<div><p><i>Spinibarbus caldwelli</i> is a commercially significant aquaculture species in China that exhibits pronounced sexual dimorphism in growth-related traits. However, identifying sex based on morphological characteristics remains challenging before sexual maturation, necessitating the development of sex-specific molecular markers for sex-controlled breeding. In this study, whole-genome resequencing was performed on 50 individuals (25 females and 25 males) with an average sequencing depth of 10.05×. A genome-wide association study (GWAS) identified 140 loci significantly associated with sex, all located within a narrow 7-kb region (26.033–26.040 Mb) on chr 46. Analysis of fixation index (FST) revealed a prominent peak within the same interval, corroborating the GWAS results and confirming a strong association between this locus and sex determination. Utilizing seven InDel polymorphisms within this region (comprising a 20 bp total deletion), we developed the primer pair P11-3. Electrophoretic genotyping using this marker demonstrated 100% accuracy, showing complete concordance between genotypes and phenotypic sex. This study fine-mapped a candidate sex-associated region in <i>S. caldwelli</i>, providing robust evidence for a male heterogametic (XX/XY) sex determination system. Furthermore, we established a simple and highly accurate molecular method for sex identification. These findings provide reliable technical support for broodstock selection, early sexing of juveniles, and germplasm improvement in <i>S. caldwelli</i>, while offering valuable insights into the mechanisms of sex determination in cyprinid fishes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Transcriptomic and Proteomic Analyses Reveal Stage-Dependent Gill Remodeling Under Chronic Salinity Stress in Grass Carp (Ctenopharyngodon idella)","authors":"Jinglin Zhu,&nbsp;Wei Xiao,&nbsp;Dayu Li,&nbsp;Jie Yu,&nbsp;Binglin Chen,&nbsp;Hong Yang,&nbsp;Zhiying Zou","doi":"10.1007/s10126-026-10651-0","DOIUrl":"10.1007/s10126-026-10651-0","url":null,"abstract":"<div><p>The expansion of brackish-water aquaculture for freshwater species such as grass carp (<i>Ctenopharyngodon idella</i>) necessitates a mechanistic understanding of their physiological and molecular responses to elevated salinity. Here, we performed integrated histopathological,transcriptomic and proteomic analyses of gill tissues from grass carp chronically exposed to 7‰ and 14‰ salinity to elucidate stage-specific responses to prolonged salinity stress. At moderate salinity (7‰), gill responses were characterized by epithelial stabilization, activation of adhesion-related pathways, enhanced carbohydrate metabolism, extracellular matrix (ECM) remodeling, and suppressed immune activity, accompanied by only mild histological alterations. In contrast, exposure to higher salinity (14‰) resulted in severe gill damage, including epithelial thickening, lamellar atrophy, vascular congestion, and hemorrhagic lesions, together with reduced survival. Multi-omics analyses revealed extensive immune, metabolic, and signaling reprogramming at 14‰, characterized by sustained immune suppression, intensified ECM remodeling, and a metabolic shift from carbohydrate utilization toward lipid- and sterol-associated pathways. Several candidates involved in apoptosis, epithelial maintenance, and sterol biosynthesis showed concordant regulation at both transcriptomic and proteomic levels. Collectively, these findings demonstrate a transition from acclimatory compensation at 7‰ to stress-associated tissue remodeling at 14‰. This study provides integrated multi-omics insights into salinity stress responses in grass carp and offers candidate pathways and genes for future studies on salinity tolerance mechanisms in freshwater aquaculture species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148196971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Complete Genome of Streptococcus agalactiae from Malaysian Aquaculture: A Closer Look at Molecular Characteristics and Phylogenomic","authors":"Helmi Husaini Zainal Fithri,&nbsp;Nurul Hidayah Samsulrizal,&nbsp;Nur-Nazifah Mansor,&nbsp;Nurasyikin Hamzah,&nbsp;Noor Hanis Abu Halim,&nbsp;Mohd Syafiq Mohammad Ridzuan,&nbsp;Azila Abdullah,&nbsp;Nur Amalin Sofea Abdul Rahim,&nbsp;Azzmer Azzar Abdul Hamid","doi":"10.1007/s10126-026-10645-y","DOIUrl":"10.1007/s10126-026-10645-y","url":null,"abstract":"<div><p><i>Streptococcus agalactiae</i> (Group B Streptococcus, GBS) is a significant pathogen in aquaculture worldwide and is responsible for high mortality in farmed fish. Despite its regional impact, complete genome data from Malaysian isolates remain scarce. In this study, we report the first complete genome of a Malaysian <i>S. agalactiae</i> isolate, SA2BKE, derived from infected tilapia. Using Oxford Nanopore long-read sequencing, we assembled a 2.03 Mb circular complete genome of <i>S. agalactiae</i>. The functional annotation revealed 1,970 protein-coding genes and 108 RNA genes. Several antimicrobial resistance genes, including <i>tet(M)</i>,<i> mreA</i>,<i> and mprF</i>, are associated with resistance to tetracyclines, macrolides, and peptides, respectively. Notably, there are 15 virulence-associated proteins involved in cell wall/membrane/envelope biogenesis. Multilocus sequence typing (MLST) identified SA2BKE as sequence type ST283, which has the potential to infect both fish and humans. Comparative phylogenomic analysis revealed 215 global strains positioned SA2BKE within a clade of other ST283 isolates from Asia and South America, suggesting potential transregional transmission. Pan-genome analysis identified 555 core genes shared among the analysed genomes, highlighting substantial genomic diversity within the species. Additionally, 14 mobile genetic element-associated regions were detected in SA2BKE, indicating potential genome plasticity and horizontal gene transfer events. These findings expand the genomic reference data for <i>S. agalactiae</i> isolates from Malaysia, contributing to regional surveillance efforts across Southeast Asia and supporting integrated disease management strategies in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148173045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunotherapeutic and Antitumoral Potential of Fatty Acids from Subantarctic Macroalgae (Sarcopeltis skottsbergii)","authors":"Gilca Costa Nachtigal,&nbsp;Bruna Silveira Pacheco,&nbsp;Fernanda Severo Sabedra Sousa,&nbsp;Maria Eduarda Ehlert,&nbsp;Tallyson Nogueira Barbosa,&nbsp;Nicole Ramos Scholl,&nbsp;Cristina Jansen Alves,&nbsp;Claudio Martin Pereira de Pereira,&nbsp;Andrés Mansilla,&nbsp;Sibele Borsuk,&nbsp;Tiago Veiras Collares,&nbsp;Fabiana Kommling Seixas","doi":"10.1007/s10126-026-10638-x","DOIUrl":"10.1007/s10126-026-10638-x","url":null,"abstract":"<div><p>Immunotherapy has emerged as a pivotal approach in cancer treatment, harnessing the body’s immune system to recognize and eradicate tumor cells. The search for novel compounds that can enhance immune responses while selectively targeting cancer cells remains a significant area of research. This study investigates the immunotherapeutic and antitumoral potential of fatty acids derived from the subantarctic red macroalgae <i>Sarcopeltis skottsbergii</i>. The cytotoxic effects of these fatty acids were evaluated in vitro using HCC1954 (breast cancer), HL-60 (leukemia), and CHO (non-tumoral) cell lines. Results demonstrated significant inhibition of cell proliferation by MTT assay in HCC1954 and HL-60 cell lines (52% and 57.9% of growth inhibition, respectively), with no observed cytotoxicity in CHO cells. Cytotoxicity was also confirmed by fluorescence assay (LIVE/DEAD). Additionally, real-time PCR analysis conducted on the J774A.1 macrophage cell line and primary culture of mice splenocytes revealed increased expression of interleukins IL-4, IL-6, IL-10, IL-12, IL-17, TNF-α, and IFN-γ, which play a pivotal role in the immune system, indicating a balanced immune response that both mitigates excessive inflammation and promotes direct antitumor activity. These findings suggest that fatty acids from <i>S. skottsbergii</i> can adapt an immune response, contributing to the immunomodulation of the tumor. The absence of cytotoxicity in non-tumoral cells, combined with selective inhibition of cancer cell growth and immune activation, highlights the potential of <i>S. skottsbergii</i> fatty acids as a promising adjuvant in cancer therapy. These results underscore the value of natural compounds from marine sources in developing innovative immunotherapeutic strategies against cancer.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-026-10638-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148172975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammonia-induced Disruption of Metabolic and Antioxidant Pathways in the Gill, Hepatopancreas, and Muscle of Macrobrachium rosenbergii","authors":"Nusrat Liaqat,&nbsp;Xuenan Li,&nbsp;Mohsan Raza,&nbsp;Kiran Shahzadi,&nbsp;Simin You,&nbsp;Shamoona Arshad,&nbsp;Mehr un Nissa,&nbsp;Nauman Khan,&nbsp;Xilin Dai","doi":"10.1007/s10126-026-10646-x","DOIUrl":"10.1007/s10126-026-10646-x","url":null,"abstract":"<div><p>Ammonia accumulation is a major constraint in intensive <i>Macrobrachium rosenbergii</i> aquaculture, yet the mechanisms linking multi-organ injury to tissue deterioration remain unclear. Juvenile prawns were exposed for 7 days to 0, 2, and 50 mg L⁻¹ total ammonia nitrogen (TAN) to evaluate integrated physiological, histological, transcriptomic, and metabolomic responses in gill, hepatopancreas, and muscle. Survival decreased from 100% in controls to 89% and 75% at 2 and 50 mg L⁻¹, respectively, accompanied by gill lamellar disruption, hepatopancreatic tubule degeneration, and muscle fiber separation. RNA-seq identified 4,944 differentially expressed genes with strong organ-specific patterns. The hepatopancreas showed enhanced lysosomal activity and redox defenses, the gills exhibited disrupted nitrogen and lipid metabolism, and the muscle displayed impaired glycolysis and altered structural protein signaling. Changes in CD63, CssYP2J, GLUL, and AIFM1 expression, together with altered CAT, GS, and GDH activities, indicated adaptive detoxification at moderate TAN. However, high TAN overwhelmed these responses, leading to glutamine detoxification failure, lipid peroxidation, and malondialdehyde accumulation. Gill metabolomics revealed TAN-dependent alterations in carbon, amino acid, and phospholipid pathways, particularly glycerol-phosphocholine, citric acid, and citrulline metabolism, highlighting metabolite–gene interactions within nitrogen and membrane lipid networks. Overall, even sublethal TAN acted as a systemic stressor, reprogramming metabolic and antioxidant pathways across organs, with muscle emerging as the most vulnerable tissue. These findings refine ammonia toxicity thresholds and support maintaining TAN below 2 mg L⁻¹ to protect prawn health and aquaculture sustainability.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148173049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stearoyl-CoA Desaturase (SCD) Gene Based Molecular Breeding Enhances Nutritional Quality and Growth Performance in the Oyster (Crassostrea angulata)","authors":"Taiping Zhang,&nbsp;Chaogang Wang,&nbsp;Rihao Cong,&nbsp;Luping Wang,&nbsp;Qingyuan Li,&nbsp;Zhuxiang Jiang,&nbsp;Mingyang Du,&nbsp;Zhong Luo,&nbsp;Haigang Qi,&nbsp;Wei Wang,&nbsp;Jincheng Chen,&nbsp;Meiqian Pang,&nbsp;Min Wang,&nbsp;Guofan Zhang,&nbsp;Li Li","doi":"10.1007/s10126-026-10643-0","DOIUrl":"10.1007/s10126-026-10643-0","url":null,"abstract":"<div><p><i>C. angulata</i> accounts for the largest share of total oyster production in China, and it shows a significant divergence from <i>C. gigas</i> in key growth and nutritional traits. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are crucial nutritional components in oysters. Oleic acid, a key precursor in the biosynthesis of polyunsaturated fatty acids via Stearoyl-CoA desaturase (SCD), represents a highly promising target for enhancing the overall polyunsaturated fatty acid profile in oysters. Previous studies identified 16 SNPs in promoter region of <i>Scd</i> gene that are associated with oleic acid biosynthesis differences between <i>C. gigas</i> and <i>C. angulata</i>. However, the potential application of these SNPs in practical breeding populations remains unclear. In this study, we validate the association between 15 strong-linkage SNPs within the target selection region of the <i>Scd</i> gene and unsaturated fatty acid content in <i>C. gigas</i> and <i>C. angulata</i> hybrid population that explain an average of 10.6% of the phenotypic variance in oleic acid. Based on SNPs Individuals carrying favorable genotypes were selected from wild <i>C. angulata</i> to establish the <i>SCDAA2024-G1</i> population. Following a single generation of selection, the oleic acid content increased significantly by 20.03%, accompanied by concurrent increases in EPA (19.27%) and DHA (11.41%), respectively. Importantly, the yield performance was synergistically improved. Integrated analyses further identified 11 key candidate genes might involve in genetic improvements. The study demonstrates the potential of molecular breeding based on targeted selection region of key gene to enhance quality and growth in bivalves.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neoagarotetraose from Marine Red Algae Ameliorates UVB-Induced Skin Barrier Damage in Mice and HaCaT Keratinocytes","authors":"Nan Wu,&nbsp;Chaocheng Wu,&nbsp;Xiao Liu,&nbsp;Ziyi Yang,&nbsp;Zhuhua Chan,&nbsp;Runying Zeng","doi":"10.1007/s10126-026-10647-w","DOIUrl":"10.1007/s10126-026-10647-w","url":null,"abstract":"<div><p>Ultraviolet B (UVB) radiation is a major environmental threat that disrupts skin integrity and drives inflammatory skin disorders. Neoagarotetraose (NA4), a low-molecular-weight oligosaccharide derived from marine red algae, is characterized by high bioavailability and well-documented anti-inflammatory properties, yet its role in UVB-induced skin damage remains unexplored. In this study, we investigated the reparative effects of NA4 and its underlying mechanisms using UVB-irradiated HaCaT keratinocytes and C57BL/6 mouse models, with focus on cytotoxicity, cell viability, barrier protein expression, pro-inflammatory cytokines, and MAPK pathway activation. Our results demonstrate that NA4 was non-cytotoxic and dose-dependently restored the viability of UVB-damaged keratinocytes. Notably, NA4 reestablished the expression of key barrier proteins (FLG, ZO-1, COL-I) and corrected UVB-induced elevation of aquaporin-3 (AQP3), while concurrently suppressing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and inhibiting the phosphorylation of JNK, ERK1/2, and p38 in both cell and animal models. In vivo, the 2 mg/cm² NA4 treatment achieved superior barrier restoration and anti-inflammatory effects compared to vitamin C. Collectively, these findings identify NA4 as a concentration-dependent, multi-target marine-derived oligosaccharide that concurrently exerts MAPK-mediated anti-inflammatory activity and barrier-restorative effects, offering a mechanistic basis for developing next-generation marine therapeutics against UVB-induced skin damage.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the Fucoidan and Mannitol Biosynthetic Pathways in the Brown Seaweed Padina Tetrastromatica Based on Draft Genome and Transcriptome Analysis","authors":"Sumit Sudhir Phakatkar,&nbsp;Ashutosh Shankar Parab,&nbsp;Soham Sudam Naik,&nbsp;Cathrine Sumathi Manohar","doi":"10.1007/s10126-026-10649-8","DOIUrl":"10.1007/s10126-026-10649-8","url":null,"abstract":"<div><p>Brown seaweeds, such as <i>Padina</i> sp., are reported to have significant potential for industrial applications due to their rich content of bioactive compounds. Despite their importance, they are not widely utilised due to insufficient biomass availability and are largely uncultivable because of their complex reproductive cycle. Hence, omics-based studies can provide a platform for future efficient biotechnological applications. However, genomic and transcriptomic insights into these seaweed species remain largely unexplored. In this study, a complementary genome and transcriptome assembly of the brown seaweed <i>Padina tetrastromatica</i> was generated to elucidate the gene repertoire and transcripts. The draft genome assembly comprised approximately 256.5 Mb across 55,222 transcripts (N50: 6,718 bp) with less than 20% coverage, while the <i>de novo</i> transcriptome assembly yielded 89,735 transcripts, with 86 and 92.5% completeness in <i>Eukaryota</i> and <i>Stramenopiles</i> assembly, respectively, based on Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis. Functional annotation revealed the presence of 15 transcripts encoding enzymes such as fucosyltransferases, sulfotransferases and mannitol-1-phosphate dehydrogenase related to fucoidan and mannitol biosynthesis, providing insights into the molecular framework underlying sulfated polysaccharide production. These findings contribute to understanding macroalgal carbohydrate metabolism and functional genomics and establish a resource that may support future metabolic engineering and synthetic biology efforts aimed at enhancing the production of macroalgal sugars in heterologous systems.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus","authors":"Fengyi Liu,&nbsp;Qilin Geng,&nbsp;Xue Sun,&nbsp;Jie Lin,&nbsp;Xuan Zhu,&nbsp;Danyang Wang,&nbsp;Xiao-Hua Zhang,&nbsp;Jingjie Hu,&nbsp;Shi Wang,&nbsp;Lisui Bao,&nbsp;Jia Lv","doi":"10.1007/s10126-026-10644-z","DOIUrl":"10.1007/s10126-026-10644-z","url":null,"abstract":"<div><p><i>Vibrio parahaemolyticus</i> (<i>V. parahaemolyticus</i>), a significant pathogen in aquatic products, necessitates rapid and precise detection to prevent foodborne disease outbreaks. Early detection of bacterial pathogens is crucial for effective prevention and control. In this study, we developed a field-deployable, “sample-in, answer-out” workflow that integrates efficient DNA extraction with visual fluorescence loop-mediated isothermal amplification (LAMP) for the on-site detection of <i>V. parahaemolyticus</i>. This extraction protocol enables efficient one-pot DNA release within 10 min, eliminating the need for additional purification steps. In addition, coupled with an optimized fluorescence LAMP assay targeting <i>V. parahaemolyticus</i>, the entire process—from sample to result—can be completed within approximately 50 min. The optimized assay exhibited high sensitivity, detecting as low as 1 copy/µL of plasmid DNA and 10 CFU per reaction from pure bacterial culture and spiked seawater samples. This extraction-amplification integrated approach demonstrates robust performance and high practicality for on-site early warning and pathogen monitoring in aquaculture safety settings.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and in Vitro Evaluation of Probiotic Traits in a Gut-Derived Bacillus cereus PX472023 from Farmed Lates Calcarifer, Asian Seabass","authors":"Amulya S G,&nbsp;Gayatri Tripathi,&nbsp;Samad Sheikh,&nbsp;R Bharathi Rathinam","doi":"10.1007/s10126-026-10642-1","DOIUrl":"10.1007/s10126-026-10642-1","url":null,"abstract":"<div><p>An indigenous autochthonous bacterium, <i>Bacillus cereus</i> PX472023 isolated from the gut of <i>Lates calcarifer</i> was evaluated for its potential probiotic property. The isolated bacteria produced hydrolytic enzyme activities such as starch, esculin and casein hydrolysis along with positive result for biofilm formation. The isolate possessed desirable features like tolerance to low pH (3), high bile salt (up to 4%), pepsin (0.3%), high saline conditions (up to 3%), along with optimum hydrophobicity, auto and co aggregation activity. In addition, the isolate exhibited strong antagonistic activity against <i>L. calcarifer</i> pathogens, including <i>Photobacterium damselae</i> subsp. <i>damselae</i>, <i>Lactococcus garvieae</i>, <i>Aeromonas veronii</i> and <i>Pseudomonas putida</i>. In addition, PCR amplification revealed the presence of two probiotic marker genes (<i>LuxS</i> gene and <i>E1 β-subunit</i> of the pyruvate dehydrogenase complex gene) as well. Draft genome assembly analyses revealed genome size to be 56,16,837 bp, with GC content of 34.97% and 5,605 protein coding sequences. Downstream analyses following the functional annotation of the genome demonstrated the presence of variety of bacteriocin gene clusters and presence of several genes in favour of probiotic potential of the isolate. Safety evaluation demonstrated that the isolate showed no haemolysis on sheep blood agar, was devoid of virulence-associated genes in its genome and did not induce mortality in the in vivo challenge study. The genomic investigation of antimicrobial resistance determinants revealed the absence of acquired antimicrobial resistance genes in the isolate, thereby alleviating primary concerns regarding horizontal transfer of resistance genes. The integration of in vitro and genomic analyses provided preliminary evidence supporting the probiotic potential of <i>Bacillus cereus</i> PX472023 in <i>L. calcarifer</i> and highlighted its prospective suitability for in vivo application in the same host under local environmental conditions.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"28 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}