Marine Biotechnology最新文献

{"title":"The New Roles of traf6 Gene Involved in the Development of Zebrafish Liver and Gonads","authors":"Hongyan Xu,&nbsp;Wenzhuo Ban,&nbsp;Jiaming Tian,&nbsp;Jianfei Xu,&nbsp;Zhimin Tan,&nbsp;Sendong Li,&nbsp;Kaili Chen,&nbsp;Mi Ou,&nbsp;Kaibin Li","doi":"10.1007/s10126-024-10329-5","DOIUrl":"10.1007/s10126-024-10329-5","url":null,"abstract":"<div><p>Traf6, an adaptor protein, exhibits non-conventional E3 ubiquitin ligase activity and was well studied as an important factor in immune systems and cancerogenesis. In mice, the traf6-null caused a perinatal death, so that the underlying pathophysiology of <i>traf6</i>-defeciency is still largely unclear in animals. Here, in the present study, a <i>traf6</i> knockout zebrafish line (<i>traf6</i>^{<i>−/−</i>}) was generated and could survive until adulthood, providing a unique opportunity to demonstrate the functions of <i>traf6</i> gene in animals’ organogenesis beyond the mouse model. The body of <i>traf6</i>^{<i>−/−</i>} fish was found to be significantly shorter than that of the wildtype (WT). Likewise, a comparative transcriptome analysis showed that 866 transcripts were significantly altered in the <i>traf6</i>^{<i>−/−</i>} liver, mainly involved in the immune system, metabolic pathways, and progesterone-mediated oocyte maturation. Especially, the mRNA expression of the pancreas duodenum homeobox protein 1 (<i>pdx1</i>), glucose-6-phosphatase (<i>g6pcb</i>), and the vitellogenesis genes (<i>vtgs</i>) were significantly decreased in the <i>traf6</i>^{<i>−/−</i>} liver. Subsequently, the glucose was found to be accumulated in the <i>traf6</i>^{<i>−/−</i>} liver tissues, and the meiotic germ cell was barely detected in <i>traf6</i>^{<i>−/−</i>} testis or ovary. The findings of this study firstly implied the pivotal functions of <i>traf6</i> gene in the liver and gonads’ development in fish species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"917 - 930"},"PeriodicalIF":2.6,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue Localization of Tetrodotoxin in the Flatworm Planocera multitentaculata (Platyhelminthes: Polycladida)","authors":"Ryo Yonezawa,&nbsp;Kentaro Hayashi,&nbsp;Hikaru Oyama,&nbsp;Kazutoshi Yoshitake,&nbsp;Soshi Sato,&nbsp;Jayan Duminda M. Senevirathna,&nbsp;Ashley R. Smith,&nbsp;Taiki Okabe,&nbsp;Rei Suo,&nbsp;Shigeharu Kinoshita,&nbsp;Tomohiro Takatani,&nbsp;Osamu Arakawa,&nbsp;Shuichi Asakawa,&nbsp;Shiro Itoi","doi":"10.1007/s10126-024-10332-w","DOIUrl":"10.1007/s10126-024-10332-w","url":null,"abstract":"<div><p>Tetrodotoxin (TTX), a pufferfish toxin, is a highly potent neurotoxin that has been found in a wide variety of animals. The TTX-bearing flatworm <i>Planocera multitentaculata</i> possesses a large amount of TTX and is considered responsible for the toxification of TTX-bearing animals such as pufferfish (<i>Takifugu</i> and <i>Chelonodon</i>) and the toxic goby <i>Yongeichthys criniger</i>. However, the mechanism underlying TTX accumulation in flatworms remains unclear. Previous studies have been limited to identifying the distribution of TTX in multiple organs, such as the digestive organs, genital parts, and the remaining tissues of flatworms. Here, we performed liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis and immunohistochemical staining using a monoclonal anti-TTX antibody to elucidate the detailed localization of TTX in the tissues and organs of the flatworm <i>P. multitentaculata</i>. Immunohistochemical staining for <i>P. multitentaculata</i> showed that TTX-specific signals were detected not only in the ovaries and pharynx but also in many other tissues and organs, whereas no signal was detected in the brain, Lang’s vesicle, and genitalia. In addition, combined with LC–MS/MS analysis, it was revealed for the first time that TTX accumulates in high concentrations in the basement membrane and epidermis. These findings robustly support the hypotheses of “TTX utilization protection from predators.”</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"649 - 657"},"PeriodicalIF":2.6,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De Novo Assembly and Annotation of the Siganus fuscescens (Houttuyn, 1782) Genome: Marking a Pioneering Advance for the Siganidae Family","authors":"Samuel Mwakisha Mwamburi,&nbsp;Satoshi Kawato,&nbsp;Miho Furukawa,&nbsp;Kayo Konishi,&nbsp;Reiko Nozaki,&nbsp;Ikuo Hirono,&nbsp;Hidehiro Kondo","doi":"10.1007/s10126-024-10325-9","DOIUrl":"10.1007/s10126-024-10325-9","url":null,"abstract":"<div><p>This study presents the first draft genome of <i>Siganus fuscescens</i>, and thereby establishes the first whole-genome sequence for a species in the Siganidae family. Leveraging both long and short read sequencing technologies, i.e., Oxford Nanopore and Illumina sequencing, we successfully assembled a mitogenome spanning 16.494 Kb and a first haploid genome encompassing 498 Mb. The assembled genome accounted for a 99.6% of the estimated genome size and was organized into 164 contigs with an N50 of 7.2 Mb. This genome assembly showed a GC content of 42.9% and a high Benchmarking Universal Single-Copy Orthologue (BUSCO) completeness score of 99.5% using actinopterygii_odb10 lineage, thereby meeting stringent quality standards. In addition to its structural aspects, our study also examined the functional genomics of this species, including the intricate capacity to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) and secrete venom. Notably, our analyses revealed various repeats elements, which collectively constituted 17.43% of the genome. Moreover, annotation of 28,351 genes uncovered both shared genetic signatures and those that are unique to <i>S. fuscescens</i>. Our assembled genome also displayed a moderate prevalence of gene duplication compared to other fish species, which suggests that this species has a distinctive evolutionary trajectory and potentially unique functional constraints. Taken altogether, this genomic resource establishes a robust foundation for future research on the biology, evolution, and the aquaculture potential of <i>S. fuscescens</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"902 - 916"},"PeriodicalIF":2.6,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141292992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Repetitive Sequences in Fish Cell Depletion as a Target for the CRISPR/Cas9 System","authors":"Yunsheng Zhang,&nbsp;Hu Xia,&nbsp;Wei Peng,&nbsp;Lanhai Liu,&nbsp;Liangguo Liu,&nbsp;Pinhong Yang","doi":"10.1007/s10126-024-10328-6","DOIUrl":"10.1007/s10126-024-10328-6","url":null,"abstract":"<div><p>Specific cell depletion is a common means to study the physiological function of cell lineages and tissue regeneration. However, 100% depletion is difficult to achieve with existing cell depletion strategies. With the increasing maturity of CRISPR/Cas9 technology, it is increasingly used for the depletion of various cells. However, even with this technology, it is difficult to complete the depletion of specific gene knockout cells. For this reason, cell depletion with the use of repetitive sequences as the target of CRISPR/Cas9 was explored using zebrafish. All cells were used as the target cells for the first set of experiments. The results showed that injection of a mixture of DANA-gRNA and Cas9 mRNA into zygotes resulted in substantial cell apoptosis. Cells are almost invisible in the embryonic animal pole during the dome stage. The activities of the caspase-3 and caspase-9 proteins and the mRNA level of the <i>P53</i> gene were significantly increased. Then, primordial germ cells (PGCs) in embryos were used as the target cells in subsequent experiments. To specifically knock out PGCs, we injected the mix of DANA-gRNA, pkop: Cas9 plasmid (the kop promotor allows Cas9 expression only in PGCs), and eGFP-nos3′UTR mRNA into zebrafish fertilized eggs. The results revealed that the activity of the caspase-3 protein was significantly increased, and the mRNA levels of <i>P53</i>, <i>ku70</i>, and <i>ku80</i> were significantly upregulated, while the number of PGCs decreased gradually. Few PGCs labeled with GFP could be seen 20 h post-fertilization (hpf), and no PGCs could be seen at the germinal ridge 24 hpf. Therefore, the combination of CRISPR/Cas9 technology and repetitive sequences can achieve efficient cell depletion regardless of whether there is generalized expression or expression in specific cells. These results indicate that it is feasible to eliminate cells by using repeat sequences as CRISPR/Cas9 system target sites.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"639 - 648"},"PeriodicalIF":2.6,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OysterDB: A Genome Database for Ostreidae","authors":"Xinchun Li,&nbsp;Yitian Bai,&nbsp;Chengxun Xu,&nbsp;Shikai Liu,&nbsp;Hong Yu,&nbsp;Lingfeng Kong,&nbsp;Shaojun Du,&nbsp;Qi Li","doi":"10.1007/s10126-024-10327-7","DOIUrl":"10.1007/s10126-024-10327-7","url":null,"abstract":"<div><p>The molluscan family Ostreidae, commonly known as oysters, is an important molluscan group due to its economic and ecological importance. In recent years, an abundance of genomic data of Ostreidae species has been generated and available in public domain. However, there is still a lack of a high-efficiency database platform to store and distribute these data with comprehensive tools. In this study, we developed an oyster genome database (OysterDB) to consolidate oyster genomic data. This database includes eight oyster genomes and 208,923 protein-coding gene annotations. Bioinformatic tools, such as BLAST and JBrowse, are integrated into the database to provide a user-friendly platform for homologous sequence searching, visualization of genomes, and screen for candidate gene information. Moreover, OysterDB will be continuously updated with ever-growing oyster genomic resources and facilitate future studies for comparative and functional genomic analysis of oysters (http://oysterdb.com.cn/).</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"827 - 834"},"PeriodicalIF":2.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary Melatonin on Antioxidant Capacity, Immune Defense, and Intestinal Microbiota in Red Swamp Crayfish (Procambarus clarkii)","authors":"Yucong Ye,&nbsp;Jiarong Huang,&nbsp;Siwen Li,&nbsp;Yiming Li,&nbsp;Yunlong Zhao","doi":"10.1007/s10126-024-10326-8","DOIUrl":"10.1007/s10126-024-10326-8","url":null,"abstract":"<div><p>The aim of this study was to investigate the effects of melatonin (MT) feed supplementation on the antioxidant capacity, immune defense, and intestinal flora in <i>Procambarus clarkii</i> (<i>P. clarkii</i>). Six groups of <i>P. clarkii</i> were fed test feeds containing different levels of MT: 0 mg/kg (control), 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg for a duration of 2 months. The specific growth rate, hepatosomatic index, and condition factor were recorded highest in the test group of shrimp fed an MT concentration of 165.1 mg/kg. Compared to the control group, the rate of apoptosis was lower in hepatopancreas cells of <i>P. clarkii</i> supplemented with high concentrations of MT. Analyses of antioxidant capacity and immune-response-related enzymes in the hepatopancreas indicated that dietary supplementation of MT significantly augmented both the antioxidant system and immune responses. Dietary MT supplementation significantly increased the expression levels of antioxidant-immunity-related genes and decreased the expression levels of genes linked to apoptosis. Dietary MT was associated with an elevation in the abundance of the <i>Firmicutes</i> and a reduction in the abundance of the <i>Proteobacteria</i> in the intestines; besides, resulting in an increase in the abundance of beneficial bacteria, such as <i>Lactobacilli</i>. The broken-line model indicated that the suitable MT concentration was 154.09–157.09 mg/kg. MT supplementation enhanced the growth performance of <i>P. clarkii</i>, exerting a positive influence on the intestinal microbiota, and bolstered both immune response and disease resistance. Thus, this study offered novel perspectives regarding the application of dietary MT supplementation within the aquaculture field.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"623 - 638"},"PeriodicalIF":2.6,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal Transcriptomic Profiling Reveals Dynamic Changes in Gene Expression of Giant Freshwater Prawn upon Acute Saline-Alkaline Stresses","authors":"Zheyan Chen,&nbsp;Shouhao Zhu,&nbsp;Bingbing Feng,&nbsp;Min Zhang,&nbsp;Jinhua Gong,&nbsp;Huangen Chen,&nbsp;Brian Pelekelo Munganga,&nbsp;Xianji Tao,&nbsp;Jianbin Feng","doi":"10.1007/s10126-024-10314-y","DOIUrl":"10.1007/s10126-024-10314-y","url":null,"abstract":"<div><p>Bicarbonate and sulfate are among two primary ion constituents of saline-alkaline water, with excessive levels potentially causing metabolic disorders in crustaceans, affecting their molting and interrupting development. As an economically important crustacean species, the molecular adaptive mechanism of giant freshwater prawn <i>Macrobrachium rosenbergii</i> in response to the stress of bicarbonate and sulfate remains unexplored. To investigate the mechanism underlying NaHCO₃, Na₂SO₄, and mixed NaHCO₃, Na₂SO₄ stresses, <i>M. rosenbergii</i> larvae were exposed to the above three stress conditions, followed by total RNA extraction and high-throughput sequencing at eight distinct time points (0, 4, 8, 12, 24, 48, 72, and 96 h). Subsequent analysis revealed 13, 16, and 13 consistently identified differentially expressed genes (DEGs) across eight time points under three stress conditions. These consistently identified DEGs were significantly involved in the Gene Ontology (GO) terms of chitin-based cuticle development, protein-carbohydrate complex, structural constituent of cuticle, carnitine biosynthetic process, extracellular matrix, and polysaccharide catabolic process, indicating that alkaline stresses might potentially impact the energy metabolism, growth, and molting of <i>M. rosenbergii</i> larvae. Particularly, the transcriptome data revealed that DEGs associated with energy metabolism, immunity, and amino acid metabolism were enriched across multiple time points under three stress conditions. These DEGs are linked to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including glycolysis/glucogenesis, amino sugar and nucleotide sugar metabolism, and lysine degradation. Consistent enrichment findings across the three stress conditions support conclusions above. Together, these insights are instrumental in enhancing our understanding of the molecular mechanisms underlying the alkaline response in <i>M. rosenbergii</i> larvae. Additionally, they offer valuable perspectives on the regulatory mechanisms of freshwater crustaceans amid saline-alkaline water development.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"511 - 525"},"PeriodicalIF":2.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis of Different Aquaculture Substrates on the Immune Response of Babylonia areolata","authors":"Jiahua Zhang,&nbsp;Jie Wang,&nbsp;Zhaojun Gu,&nbsp;Xingguo Liu","doi":"10.1007/s10126-024-10324-w","DOIUrl":"10.1007/s10126-024-10324-w","url":null,"abstract":"<div><p>To assess the impact of different substrates in a recirculating water system on the immune response and antioxidant capacity of <i>Babylonia areolata</i>, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand—S group, ceramic granules—C group, and PVC breeding nest—P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism—other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in <i>B. areolata</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"609 - 622"},"PeriodicalIF":2.6,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein and Polysaccharide Recovery from Shrimp Wastes by Natural Deep Eutectic Solvent Mediated Subcritical Water Hydrolysis for Biodegradable Film","authors":"Md Sadek Ali,&nbsp;Vikash Chandra Roy,&nbsp;Jin-Seok Park,&nbsp;Ahmed Redwan Haque,&nbsp;Jin Hong Mok,&nbsp;Wei Zhang,&nbsp;Byung-Soo Chun","doi":"10.1007/s10126-024-10321-z","DOIUrl":"10.1007/s10126-024-10321-z","url":null,"abstract":"<div><p>Environmental pollution is a significant problem due to the improper disposal of plastics and shrimp shells outdoors. Therefore, the synthesis of biodegradable film from waste materials is highly important. The novelty of this research lies in the extraction of protein hydrolysates and chitosan from shrimp shells, as well as the fabrication of biodegradable film from these materials. In this study, the composite films were produced using the solution casting method. Moreover, the combined effect of ultrasound pretreatments (UPT) and natural deep eutectic solvents (NADES) was investigated as extraction media, to determine their potential impact on shrimp waste subcritical water hydrolysis (SWH). Shrimp shells were submitted to UPT in NADES solution, followed by SWH at different temperatures ranging from 150 to 230 °C under 3 MPa for 20 min. Then, the physiochemical properties and bioactivities of the hydrolysates were assessed to determine their suitability for use in biodegradable packaging films. Additionally, the physiochemical properties and bioactivities of the resulting hydrolysates were also analyzed. The highest amount of protein (391.96 ± 0.48 mg BSA/g) was obtained at 190 °C/UPT/NADES, and the average molecular size of the protein molecules was less than 1000 Da with different kinds of peptide. Overall, combined UPT and SWH treatments yielded higher antioxidant activity levels than individual treatments. Finally, the application of composite films was evaluated by wrapping fish samples and assessing their lipid oxidation. The use of higher concentrations of protein hydrolysates significantly delayed changes in the samples, thereby demonstrating the film’s applicability.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"876 - 890"},"PeriodicalIF":2.6,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Characteristics and Suitability of Sterile dead end Knockout Nibe Croaker as a Recipient for Intraperitoneal Germ Cell Transplantation","authors":"Ryosuke Yazawa,&nbsp;Kyoichiro Saitoh,&nbsp;Akihiro Yamauchi,&nbsp;Onur Eyüboğlu,&nbsp;Kana Ozawa,&nbsp;Wataru Kawamura,&nbsp;Tetsuro Morita,&nbsp;Yutaka Takeuchi,&nbsp;Goro Yoshizaki","doi":"10.1007/s10126-024-10323-x","DOIUrl":"10.1007/s10126-024-10323-x","url":null,"abstract":"<div><p>The use of sterile recipients is crucial for efficiently producing donor-derived offspring through surrogate broodstock technology for practical aquaculture applications. Although knockout (KO) of the <i>dead end</i> (<i>dnd</i>) gene has been used in previous studies as a sterilization method, it has not been reported in marine fish. In this study, nibe croaker was utilized as a model for marine teleosts that produce small pelagic eggs, and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system was utilized to produce <i>dnd</i> KO fish. The F1 generation, which carried a nonsense mutation in the <i>dnd</i> gene, was produced by mating founder individuals with wild-type counterparts. Subsequently, the F2 generation was produced by mating the resulting males and females. Among the F2 generations, 24.0% consisted of homozygous KO individuals. Histological analysis revealed that primordial germ cells (PGCs) were present in homozygous KO individuals at 10 days post-hatching (dph), similar to wild-type individuals. However, by 20 dph, PGCs were absent in KO individuals. Furthermore, no germ cells were observed in the gonads of both sexes of homozygous KO individuals at 6 months old, which is the typical maturity age for wild-type individuals of both sexes. In addition, when cryopreserved donor nibe croaker testicular cells were transplanted, only donor-derived offspring were successfully obtained through the spontaneous mating of homozygous KO recipient parents. Results indicate that <i>dnd</i> KO nibe croaker lacks germ cells and can serve as promising recipients, producing only donor-derived gametes as surrogate broodstock.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"891 - 901"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140837763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}