Marine Biotechnology最新文献

{"title":"Chemical Survey and Antifungal Efficacy of Sargassum muticum’s Alkaloids and Phenolic-Rich Fraction Against Airborne Toxigenic and Nosocomial Opportunistic Molds Isolates","authors":"Houda Younssi Tarhzouti,&nbsp;Badr-ddine El mouns,&nbsp;Hayat Ben-saghroune,&nbsp;Sara Haida,&nbsp;Selma Mabrouki,&nbsp;Fatima Lakhdar,&nbsp;Samira Etahiri","doi":"10.1007/s10126-024-10376-y","DOIUrl":"10.1007/s10126-024-10376-y","url":null,"abstract":"<div><p>The Atlantic coastline of El-Jadida, Morocco, is renowned for its plentiful algae, especially brown seaweed, which is rich in active compounds known for their antifungal properties. This valuable resource offers an exciting opportunity to tackle the numerous challenges posed by invasive fungal infections, allergies, mycotoxin-related food poisoning, and drug-resistant strains. Underscoring the urgent need to explore alternative, sustainable, and environmentally friendly antifungal agents derived from algae. This study aimed to evaluate the antifungal activity of total alkaloids and phenolic-rich fractions derived from seven species of Pheophyceae: <i>Sargassum muticum</i>, <i>Sargassum vulgare</i>, <i>Bifurcaria bifurcata</i>, <i>Cystoseira tamariscifolia</i>, <i>Cystoseira humilis</i>, <i>Laminaria ochroleuca</i>, and <i>Fucus spiralis</i> against four fungi: airborne toxigenic isolates of <i>Aspergillus westerdijkiae</i> and <i>Chaetomium globosum</i> as well as nosocomial opportunistic isolates of <i>Aspergillus nidulans</i> and <i>Scopulariopsis brevicaulis</i>. The study also aimed to identify the most effective alga and its specific active compounds through LC–MS and GC–MS analysis. The invasive <i>Sargassum muticum</i> was chosen as the most potent alga in inhibiting the growth of mycelium. For the first time, the alkaloids palmatine and jatrorrhizine, along with caulerpin, have been identified. The chloroform fraction revealed the prevalence of phenolic compounds including, phenolic acids, flavonoids, and phlorotannins. The lowest minimum inhibitory concentrations (MICs), with a maximum fungal load of 10⁸ colony-forming unit (CFU), recorded ranged from 3.12 to 6.25 μg/mL by the phenolic-rich fraction against airborne toxigenic isolates, and from 100 to 200 μg/mL against nosocomial opportunistic isolates by the total alkaloids. In comparison, the positive control, ketoconazole, showed higher MICs and resistance against <i>A. nidulans</i>. The valorization of <i>Sargassum muticum</i> is proposed as a green strategy to preserve the ecological balance, combat antifungal resistance, and address public health challenges.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1350 - 1366"},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidermal distribution of tetrodotoxin-rich cells in newly hatched larvae of Takifugu spp.","authors":"Keishiro Inahashi,&nbsp;Ryo Yonezawa,&nbsp;Kentaro Hayashi,&nbsp;Soichi Watanabe,&nbsp;Kazutoshi Yoshitake,&nbsp;Ashley Rinka Smith,&nbsp;Yui Kaneko,&nbsp;Inori Watanabe,&nbsp;Rei Suo,&nbsp;Shigeharu Kinoshita,&nbsp;Muhammad Ahya Rafiuddin,&nbsp;Yuki Seki,&nbsp;Arata Nagami,&nbsp;Hajime Matsubara,&nbsp;Nobuo Suzuki,&nbsp;Tomohiro Takatani,&nbsp;Osamu Arakawa,&nbsp;Miwa Suzuki,&nbsp;Shuichi Asakawa,&nbsp;Shiro Itoi","doi":"10.1007/s10126-024-10377-x","DOIUrl":"10.1007/s10126-024-10377-x","url":null,"abstract":"<div><p>Pufferfish of the genus <i>Takifugu</i> possess tetrodotoxin (TTX), known as “pufferfish toxin” and it is believed that pufferfish eggs and newly hatched larvae utilize TTX as a defensive substance against predators. However, the mechanism for the placement of TTX to specific cells on the larval body surface during the developmental process remains unknown. In this study, we clarify the distribution and characteristics of TTX-rich cells. We performed whole-mount immunohistochemistry (IHC) using anti-TTX monoclonal antibody on larvae of two pufferfish species, <i>Takifugu rubripes</i> and <i>Takifugu alboplumbeus</i>, just after hatching. This allowed observation of the TTX location and compared it with those of wheat germ agglutinin (WGA)-positive (periodic acid-Schiff (PAS)-positive) cells for mucous cells and IHC using anti-Na⁺/K⁺-ATPase (NKA) monoclonal antibody for ionocytes. As a result, uniformly scattered localization of TTX-rich cells was commonly observed in the epidermis of the larvae of the two <i>Takifugu</i> species. TTX-rich cells were WGA-negative (PAS-negative) and structurally distinct from NKA-positive cells, suggesting that TTX-rich cells are unreported small cells unique to pufferfish skin, but not mucous cells nor ionocytes. </p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1367 - 1374"},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10377-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Mining Analysis Uncovers the Previously Unknown Biosynthetic Capacity for Secondary Metabolites in Verrucomicrobia","authors":"Xue Di,&nbsp;Peng Li,&nbsp;Jingxuan Wang,&nbsp;Vincent Nowak,&nbsp;Shuai Zhi,&nbsp;Meiling Jin,&nbsp;Liwei Liu,&nbsp;Shan He","doi":"10.1007/s10126-024-10374-0","DOIUrl":"10.1007/s10126-024-10374-0","url":null,"abstract":"<div><p>Bacteria of the phylum <i>Verrucomicrobia</i> is widely distributed in diverse ecological environments. Their limited cultivability has greatly caused the significant knowledge gap surrounding their secondary metabolites and their mediating ecological functions. This study delved into the diversity and novelty of secondary metabolite biosynthetic gene clusters (BGCs) of <i>Verrucomicrobia</i> by employing a gene-first approach to investigate 2323 genomes. A total of 7552 BGCs, which encompassed 3744 terpene, 805 polyketide, 773 non-ribosomal peptide gene clusters, and 1933 BGCs of other biosynthetic origins, were identified. They were further classified into 3887 gene cluster families (GCFs) based on biosynthetic gene similarity clustering, of which only six GCFs contained reference biosynthetic gene clusters in the Minimum Information about a Biosynthetic Gene Cluster (MIBiG), indicating the striking novelty of secondary metabolites in <i>Verrucomicrobia</i>. Notably, 37.8% of these gene clusters were harbored by unclassified species of <i>Verrucomicrobia</i> phyla, members of which were highly abundant in soil environments. Furthermore, our comprehensive analysis also revealed <i>Luteolibacter</i> and <i>Methylacidiphilum</i> as the most prolific genera in terms of BGC abundance and diversity, with the discovery of a conservative and new NRPS-PKS BGC in <i>Luteolibacter</i>. This work not only unveiled the biosynthetic potential and genetic diversity of secondary metabolites of <i>Verrucomicrobia</i> but also provided a fresh insight for the exploration of new bioactive compounds.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1324 - 1335"},"PeriodicalIF":2.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary 5-Aminolevulinic Acid on Growth, Nutrient Composition, and Intestinal Microflora in Juvenile Shrimp, Litopenaeus vannamei","authors":"Yiming Li,&nbsp;Yucong Ye,&nbsp;Yashi Zhu,&nbsp;Zongli Yao,&nbsp;Kai Zhou,&nbsp;Yuxing Wei,&nbsp;Lin Zhang,&nbsp;Ning Bao,&nbsp;Yunlong Zhao,&nbsp;Qifang Lai","doi":"10.1007/s10126-024-10373-1","DOIUrl":"10.1007/s10126-024-10373-1","url":null,"abstract":"<div><p>5-Aminolevulinic acid (5-ALA) is an endogenous non-protein amino acid and has been used as a new type of growth promoter in aquaculture feed. This study explored the effects of 5-ALA on growth and intestinal health in juvenile shrimp, <i>Litopenaeus vannamei</i>. Shrimps were fed diets containing five different 5-ALA levels (0, 15, 30, 45, and 60 g/t) for 90 days. A concentration of 45 g/t 5-ALA significantly improved growth metrics, including the specific growth rate, protein efficiency, and feed conversion (<i>P</i> &lt; 0.05). The optimal concentration of 5-ALA was 38.3 g/t, as indicated by broken-line regression. Dietary supplementation with 5-ALA increased the crude protein content of whole shrimp, but had no significant effect on the moisture, ash, or crude lipid content (<i>P</i> &gt; 0.05). Suitable supplementation of 5-ALA (45 g/t, 60 g/t) improved the activities of the digestive enzymes alpha-amylase, pepsin, trypsin, and lipase, thus promoting digestion and absorption. Shrimp fed with 45 g/t 5-ALA had increased levels of essential amino acids in the muscles and a higher proportion of polyunsaturated fatty acids in the hepatopancreas. Supplementation with 45 or 60 g/t 5-ALA upregulated the expression of genes related to growth and molting, including <i>chitinase</i>, <i>ecdysone receptor</i>, <i>retinoic X receptor</i>, <i>calcium/calmodulin-dependent protein kinase I</i>, <i>heat shock protein 60</i>, and <i>heat shock protein 70</i>. Moreover, dietary supplementation with 5-ALA affected the abundance of intestinal flora, increased the number of beneficial bacteria, and improved intestinal health. These results indicated that 5-ALA may significantly benefit shrimp health and aquaculture productivity, providing a novel theoretical basis for further research into 5-ALA as a dietary supplement.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1307 - 1323"},"PeriodicalIF":2.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Characterization of ABC Transporter Genes and Expression Profiles in Red Macroalga Pyropia yezoensis Expose to Low-Temperature","authors":"Lin Tian,&nbsp;Lan Wu,&nbsp;Xue-Feng Zhong,&nbsp;Li-Hong Ma,&nbsp;Guo-Ying Du","doi":"10.1007/s10126-024-10355-3","DOIUrl":"10.1007/s10126-024-10355-3","url":null,"abstract":"<div><p><i>Pyropia yezoensis</i> is an important economic macroalga widely cultivated in the East Asia countries of China, Korea, and Japan. The ATP-binding cassette (ABC) transporter gene family is one of the largest transporter families in all forms of life involved in various biological processes. The characteristics of ABC transporter genes in <i>P</i>. <i>yezoensis</i> (<i>PyABC</i>) and their functions in stress resistance, however, remain largely unknown. In this study, <i>PyABCs</i> were identified and characterized their expression patterns under low-temperature stress. A total of 48 PyABCs transporters were identified and divided into eight subfamilies, which are mostly predicted as membrane-binding proteins. The cis-elements of phytohormone and low-temperature response were distinguished in promoter sequences of <i>PyABCs.</i> Transcriptome analysis showed that <i>PyABCs</i> are involved in response to low-temperature stress. Among them, 12 <i>PyABCs</i> were significantly up-regulated after 24 h of exposure to low temperature (2 °C). Further quantitative RT-PCR analysis corroborated the highest expression happened at 24 for detected genes of <i>PyABCC8</i>, <i>PyABCF3</i>, and <i>PyABCI1</i>, extraordinarily for <i>PyABCF3</i>, and followed by decreased expression at 48 h. The expression of <i>PyABCI1</i> was generally low in all tested strains. Whereas, in a strain of <i>P</i>. <i>yezoensis</i> with lower tolerance to low temperature, the expression was observed higher in <i>PyABCC1</i>, <i>PyABCC8</i>, and remarkably high in <i>PyABCF3</i>. This study provided valuable information on <i>ABC</i> gene families in <i>P</i>. <i>yezoensis</i> and their functional characteristics, especially on low-temperature resistance, and would help to understand the adaptive mechanisms of <i>P</i>. <i>yezoensis</i> to adverse environments.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1179 - 1193"},"PeriodicalIF":2.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Novel β-Defensin Gene in Gilthead Seabream (Sparus aurata)","authors":"M. Ferez-Puche,&nbsp;Jhon A. Serna-Duque,&nbsp;Alberto Cuesta,&nbsp;Álvaro Sánchez-Ferrer,&nbsp;María Ángeles Esteban","doi":"10.1007/s10126-024-10367-z","DOIUrl":"10.1007/s10126-024-10367-z","url":null,"abstract":"<div><p>The excessive use of antibiotics in aquaculture favors the natural selection of multidrug-resistant bacteria, and antimicrobial peptides (AMPs) could be a promising alternative to this problem. The most studied AMPs in teleost fish are piscidins, hepcidins, and β-defensins. In this work, we have found a new gene (<i>defb2</i>) encoding a type 2 β-defensin in the genome of gilthead seabream, a species chosen for its economic interest in aquaculture. Its open reading frame (192 bp) encodes a protein (71 amino acids) that undergoes proteolytic cleavage to obtain the functional mature peptide (42 amino acids). The genetic structure in three exons and two introns and the six characteristic cysteines are conserved as the main signature of this protein family. In the evolutionary analysis, synteny shows a preservation of chromosomal localization and the phylogenetic tree constructed exposes the differences between both types of β-defensin as well as the similarities between seabream and European seabass. In relation to its basal expression, β-defensin 2 is mostly expressed in the intestine, thymus, skin, and gonads of the gilthead seabream (<i>Sparus aurata</i>). In head kidney leucoytes (HKLs), the expression was very low and did not change significantly when stimulated with various immunocompetent agents. However, the expression was significantly down-regulated in the liver, head–kidney, and blood 4 h post-injection with the fish pathogen <i>Vibrio harveyi</i>. When infected with nodavirus, the expression was downregulated in brain at 7 days post-infection. These results denote a possible complementarity between the expression patterns of β-defensins and hepcidins. Further studies are needed to analyze gene duplications and expression patterns of β-defensins and describe their mechanism of action in seabream and other teleost fish.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1219 - 1230"},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10367-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chnoospora minima: a Robust Candidate for Hyperglycemia Management, Unveiling Potent Inhibitory Compounds and Their Therapeutic Potential","authors":"Thilina Lakmini Gunathilaka,&nbsp;Upeka Bandaranayake,&nbsp;Mohamad Boudjelal,&nbsp;Rizwan Ali,&nbsp;Rajitha M. Silva,&nbsp;Kalpa W. Samarakoon,&nbsp;Pathmasiri Ranasinghe,&nbsp;L. Dinithi C. Peiris","doi":"10.1007/s10126-024-10368-y","DOIUrl":"10.1007/s10126-024-10368-y","url":null,"abstract":"<div><p>The present study aimed to isolate a bioactive compound from Sri Lankan edible marine brown algae, <i>Chnoospora minima</i>, to manage diabetes. The de-polysaccharide crude methanolic extract was partitioned using hexane, chloroform, and ethyl acetate with increased polarity. The samples were subjected to determine the quantitative phytochemical analysis, antioxidants, and antidiabetic potentials. Further, the potent antidiabetic fraction was selected to isolate an active compound using bioactivity-guided fractionation. From the selected extract, the chloroform fraction exhibited comparatively high TPC (59.01 ± 1.86 mg GAE/g), TFC (5.14 ± 0.43 mg QE/g) and alkaloid content (2.79 ± 0.31 PE/g of extract). Crude methanol extract exhibited a potent DPPH activity (IC₅₀: 0.48 ± 0.01 mg/mL) whereas the ethyl acetate fraction elicited a maximum ABTS activity (IC₅₀: 0.064 ± 0.001 mg/mL) and a ferrous iron–chelating capacity (IC₅₀: 0.019 mg/mL). Similarly, the chloroform fraction exhibited the highest FRAP (20.34 ± 1.72 mg TE/g) and ORAC (19.72 ± 2.92 mg TE/g) capacities. The potent inhibitory activity of α-amylase (IC₅₀:3.17 ± 0.02 µg/mL) and α-glucosidase (IC₅₀: 1.99 ± 0.01 µg/mL) enzymes and glucose diffusion was observed in the chloroform fraction. Similarly, the chloroform extract exhibited a potent BSA-glucose (IC₅₀: 202.43 ± 5.71 µg/mL), BSA-MGO (IC₅₀: 124.30 ± 2.85 µg/mL) antiglycation model and reversing activities (EC_50BSAglucose: 98.99 ± 0.35 µg/mL; EC_50BSA-MGO: 118.89 ± 1.58 µg/mL). Depending on the hypoglycemic activity, fucoxanthin was isolated as the active compound which showed a notable change in the functional group. Molecular docking studies were conducted on the compound, and binding energy was observed to be − 6.56 kcal/mol and − 4.83 kcal/mol for α-amylase and α-glucosidase enzymes, respectively, which confirmed the hypoglycemic effect of the isolated compounds. However, more studies are required to understand the mechanistic insights of these observations.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1231 - 1245"},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and Purification of Biopolymers from Marine Origin Sources Envisaging Their Use for Biotechnological Applications","authors":"Duarte Nuno Carvalho,&nbsp;Cristiana Gonçalves,&nbsp;Rita O. Sousa,&nbsp;Rui L. Reis,&nbsp;J. Miguel Oliveira,&nbsp;Tiago H. Silva","doi":"10.1007/s10126-024-10361-5","DOIUrl":"10.1007/s10126-024-10361-5","url":null,"abstract":"<div><p>Biopolymers are a versatile and diverse class of materials that has won high interest due to their potential application in several sectors of the economy, such as cosmetics, medical materials/devices, and food additives. In the last years, the search for these compounds has explored a wider range of marine organisms that have proven to be a great alternative to mammal sources for these applications and benefit from their biological properties, such as low antigenicity, biocompatibility, and biodegradability, among others. Furthermore, to ensure the sustainable exploitation of natural marine resources and address the challenges of 3R’s policies, there is a current necessity to valorize the residues and by-products obtained from food processing to benefit both economic and environmental interests. Many extraction methodologies have received significant attention for the obtention of diverse polysaccharides, proteins, and glycosaminoglycans to accomplish the increasing demands for these products. The present review gives emphasis to the ones that can be obtained from marine biological resources, as agar/agarose, alginate and sulfated polysaccharides from seaweeds, chitin/chitosan from crustaceans from crustaceans, collagen, and some glycosaminoglycans such as chondroitin sulfate and hyaluronic acids from fish. It is offered, in a summarized and easy-to-interpret arrangement, the most well-established extraction and purification methodologies used for obtaining the referred marine biopolymers, their chemical structure, as well as the characterization tools that are required to validate the extracted material and respective features. As supplementary material, a practical guide with the step-by-step isolation protocol, together with the various materials, reagents, and equipment, needed for each extraction is also delivered is also delivered. Finally, some remarks are made on the needs still observed, despite all the past efforts, to improve the current extraction and purification procedures to achieve more efficient and green methodologies with higher yields, less time-consuming, and decreased batch-to-batch variability.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1079 - 1119"},"PeriodicalIF":2.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10361-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic Genome Activation (EGA) Occurred at 1-Cell Stage of Embryonic Development in the Mud Crab, Scylla paramamosain, Revealed by RNA-Seq","authors":"Li-Kun Xu,&nbsp;Chun-Yan Ma,&nbsp;Feng-Ying Zhang,&nbsp;Wei Wang,&nbsp;Ming Zhao,&nbsp;Xin Jin,&nbsp;Jin-Ju Yin,&nbsp;Ling-Bo Ma,&nbsp;Wei Chen,&nbsp;Jia-Yuan Xu,&nbsp;Ke-Yi Ma,&nbsp;Zhi-Qiang Liu","doi":"10.1007/s10126-024-10369-x","DOIUrl":"10.1007/s10126-024-10369-x","url":null,"abstract":"<div><p>As a prerequisite for the success of embryo development, embryonic genome activation (EGA) is an important biological event in which zygotic gene products in the embryo are activated to replace maternal-derived transcripts. Although EGA has been extensively studied in a large number of vertebrates and invertebrates, there is a lack of information regarding this event in crustacean crab. In this study, the timing of EGA was confirmed by examining a transcriptomic dataset of early embryonic development, including mature oocytes and embryos through six early developmental stages, and signaling pathways associated with EGA were identified in the mud crab, <i>S. paramamosain.</i> The comprehensive transcriptomic data identified a total of 53,915 transcripts from these sequencing samples. Notable transcriptomic change was evident at the 1-cell stage, indicated by a 36% transcript number shift and a reduction in transcript fragment length, compared to those present in the mature oocytes. Concurrently, a substantial increase in the expression of newly transcribed transcripts was observed, with gene counts reaching 3485 at the 1-cell stage, indicative of the onset of EGA. GO functional enrichment revealed key biological processes initiated at the 1-cell stage, such as protein complex formation, protein metabolism, and various biosynthetic processes. KEGG analysis identified several critical signaling pathways activated during EGA, including the “cell cycle,” “spliceosome,” “RNA degradation”, and “RNA polymerase”, pathways. Furthermore, transcription factor families, including zinc finger, T-box, Nrf1, and Tub were predominantly enriched at the 1-cell stage, suggesting their pivotal roles in regulating embryonic development through the targeting of specific DNA sequences during the EGA process. This groundbreaking study not only addresses a significant knowledge gap regarding the developmental biology of <i>S. paramamosain</i>, especially for the understanding of the mechanism underlying EGA, but also provides scientific data crucial for the research on the individual synchronization of seed breeding within <i>S. paramamosain</i> aquaculture. Additionally, it serves as a reference basis for the study of early embryonic development in other crustacean species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1246 - 1259"},"PeriodicalIF":2.6,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Pluripotent Stem Cells from Muscle Cells of Large Yellow Croaker (Larimichthys Crocea) Via Electrotransfection","authors":"Zhaowei Zhong,&nbsp;Yilei Wang,&nbsp;Yan Feng,&nbsp;Yan Xu,&nbsp;Pengfei Zou,&nbsp;Ziping Zhang,&nbsp;Yonghua Jiang","doi":"10.1007/s10126-024-10372-2","DOIUrl":"10.1007/s10126-024-10372-2","url":null,"abstract":"<div><p>Induced pluripotent stem cells (iPSCs) are a new type of pluripotent cells reprogrammed from somatic cells back into an embryonic-like pluripotent state of stem cells to study development, disease and potential gene therapies. The induction and regulation mechanisms of iPSCs in fish are still unclear. By using the transfection technique, we investigated the crucial function of the OSKMNL factor co-expression for somatic reprogramming in the muscle cell line of large yellow croaker (<i>Larimichthys crocea</i>) (LYCMs) and successfully established a stable iPSCs line (<i>Lc</i>-OSNL-iPSCs). Stable culturing of iPSCs with high alkaline phosphatase activity and a stable karyotype was achieved. The qRT-PCR and immunofluorescence labeling results revealed that <i>Lc-</i>OSNL-iPSCs displayed a high expression level of pluripotent marker genes such as <i>Nanog</i>, <i>Oct4</i>, and <i>Sox2</i>. There were significant differences between <i>Lc-</i>OSNL-iPSCs, <i>Lc-</i>OSKMNL-iPSCs, and LYCMs, and the expression of several genes in maintaining cell pluripotency was up-regulated when the pluripotency signal pathway of stem cells was activated. The technical system for inducing iPSCs of <i>Larimichthys crocea</i> was constructed in this study. This system can serve as a basic model to understand germ cell differentiation mechanism, gender control, genetics, and breeding of large yellow croaker and a platform for studying iPSCs in fish. Interestingly, the acquired iPSCs serves as a useful material for the directional induction of muscle stem cells, thereby establishing the groundwork for obtaining \"artificial fish\" in the future.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1287 - 1306"},"PeriodicalIF":2.6,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}