Marine Biotechnology最新文献

{"title":"A New Investigation to Discriminate Sexes in Alive Nile Tilapia (Oreochromis niloticus) Using Cyp19a1a and Dmrt1 Gene Expression in Tail Fin Tissues","authors":"Samy Y. El-Zaeem,&nbsp;Amr El-Hanafy,&nbsp;Alaa A. El-Dahhar,&nbsp;Ayaat M. Elmaghraby,&nbsp;Amany M. Hendy","doi":"10.1007/s10126-024-10340-w","DOIUrl":"10.1007/s10126-024-10340-w","url":null,"abstract":"<div><p>The Nile Tilapia (<i>Oreochromis niloticus</i>), a gonochoristic teleost fish with a XX/XY sex-determination system, is an ideal model for investigating gonadal sex differentiation. During gonadal differentiation, the expression of <i>cyp19a1a</i> in XX gonads and <i>dmrt1</i> in XY gonads are required for undifferentiated tissues to develop into ovary or testis. In this study, quantitative real-time RT-PCR assessed the expression of <i>cyp19a1a</i> and <i>dmrt1</i> genes in gonads and tail fin tissues. Differences in gene expression mean among sexually differentiated fish were analyzed using two-way analysis of variance (ANOVA) and validation of mixed model using discriminant analysis (DA) for morphometric traits and the gene expression in gonads and tail fin tissues used to validate and utilize them in discriminating sexes in sex-differentiated Nile Tilapia fish. The results revealed that, <i>cyp19a1a</i> gene expression in female ovaries was more significant than <i>dmrt1</i> in male testis. In the other hand, the <i>dmrt1</i> gene expression in the tail fin was higher in males than females. Both, <i>cyp19a1a</i> and <i>dmrt1</i> genes, can discriminate fish sexes by 100% by using their expression in tail fin tissues. In conclusion, the <i>cyp19a1a</i> and <i>dmrt1</i> genes could be used as a genetic marker to discriminate between the Nile Tilapia sexes, whereas used as an indicator for ovarian or testis differentiation in sexually differentiated Nile Tilapia using tail fin tissues. It is worth mentioning that this is the first investigation for using <i>cyp19a1a</i> and <i>dmrt1</i> genes from Nile Tilapia tail fin tissues in sex determination.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"943 - 950"},"PeriodicalIF":2.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10340-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Selection of Large Yellow Croaker (Larimichthys crocea) with a High Plant Protein Diet Enhances the Growth Performance of Offspring","authors":"Qiaozhen Ke,&nbsp;Jiaxing Liu,&nbsp;Ji Zhao,&nbsp;Jiaying Wang,&nbsp;Pengxin Jiang,&nbsp;Yacheng Deng,&nbsp;Xiaoying Zhou,&nbsp;Junjia Zeng,&nbsp;Tao Zhou,&nbsp;Peng Xu","doi":"10.1007/s10126-024-10341-9","DOIUrl":"10.1007/s10126-024-10341-9","url":null,"abstract":"<div><p>Fishmeal is over-represented in the diets of large yellow croaker (<i>Larimichthys crocea</i>), and this farming mode, which relies heavily on fishmeal, is highly susceptible to the price of fishmeal and is unsustainable. Therefore, more and more studies on the large yellow croaker tend to replace fishmeal with land-based animal or plant proteins, but few studies have considered it from the genomic selection. In this study, we evaluated the survival rate (SR), final body weight (FBW), body weight gain (BWG), weight gain rate (WGR), and specific growth rate (SGR) of the large yellow croaker GS7 strain, which was obtained through genomic selection for tolerance to plant proteins and analyzed the differences in plant protein utilization between the GS7 strain and unselected commercial large yellow croaker (control group). The results of separate feeding for 60 days showed that although there was no significant difference in SR between the control and GS7 strains (<i>P</i> &gt; 0.05), the BWG, WGR, and SGR of the control were significantly lower (<i>P</i> &lt; 0.05) than those of the GS7 group. Results of mixed feeding after PIT marking showed that compared to the control fish, the GS7 strain had significantly higher BWG, WGR, and SGR (<i>P</i> &lt; 0.0001). To make the experimental results more precise, we compared fishes with equivalent initial body weight (IBW) in the GS7 strain and the control group. The final fish body weight (FBW) of Ctrl-2 (IBW 300–399 g) and Ctrl-4 (IBW 500–599 g) was significantly lower than those of the corresponding GS7-2 and GS7-4 (<i>P</i> &lt; 0.05), while the FBW of Ctrl-1 (IBW 200–299 g) and Ctrl-3 (IBW 400–499 g) was much significantly lower than the corresponding GS7-1 and GS7-3 (<i>P</i> &lt; 0.01). The BWG, WGR, and SGR of Ctrl-1 and Ctrl-4 were more significantly lower than those of the corresponding GS7-1 and GS7-4 (<i>P</i> &lt; 0.01), while the BWG, WGR, and SGR of Ctrl-2 and Ctrl-3 were more significantly different from the corresponding GS7-2 and GS7-3 (<i>P</i> &lt; 0.0001). Our results seem to point toward the same conclusion that the GS7 strain is better adapted to high plant protein diets than the unselected commercial large yellow croaker. These results will provide a reference for the low-fishmeal culture industry of large yellow croakers and the selection and breeding of strains tolerant to a high percentage of plant proteins in other marine fishes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"732 - 740"},"PeriodicalIF":2.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action","authors":"Athira P. P.,&nbsp;Anju M. V.,&nbsp;Anooja V. V.,&nbsp;Archana K.,&nbsp;Neelima S.,&nbsp;Muhammed Musthafa S.,&nbsp;I. S. Bright Singh,&nbsp;Rosamma Philip","doi":"10.1007/s10126-024-10338-4","DOIUrl":"10.1007/s10126-024-10338-4","url":null,"abstract":"<div><p>Antimicrobial peptides (AMPs), including beta-defensin from fish, are a crucial class of peptide medicines. The focus of the current study is the molecular and functional attributes of <i>Cm</i>Def, a 63-amino acid beta-defensin AMP from Malabar trevally, <i>Carangoides malabaricus</i>. This peptide demonstrated typical characteristics of AMPs, including hydrophobicity, amphipathic nature, and +2.8 net charge. The <i>Cm</i>Def was recombinantly expressed and the recombinant peptide, r<i>Cm</i>Def displayed a strong antimicrobial activity against bacterial fish pathogens with an MIC of 8 µM for <i>V. proteolyticus</i> and 32 µM for <i>A. hydrophila</i>. The <i>E. tarda</i> and <i>V. harveyi</i> showed an inhibition of 94% and 54%, respectively, at 32 µM concentration. No activity was observed against <i>V. fluvialis</i> and <i>V. alginolyticus.</i> The r<i>Cm</i>Def has a multimode of action that exerts an antibacterial effect by membrane depolarization followed by membrane permeabilization and ROS production. r<i>Cm</i>Def also exhibited anti-cancer activities in silico without causing hemolysis. The peptide demonstrated stability under various conditions, including different pH levels, temperatures, salts, and metal ions (KCl and CaCl₂), and remained stable in the presence of proteases such as trypsin and proteinase K at concentrations up to 0.2 µg/100 µl. The strong antibacterial efficacy and non-cytotoxic nature suggest that r<i>Cm</i>Def is a single-edged sword that can contribute significantly to aquaculture disease management.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"696 - 715"},"PeriodicalIF":2.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and Transcriptomic Insights into the Ovary Development of Hemibarbus labeo Injected with Spawn-Inducing Hormones","authors":"Xinming Gao,&nbsp;Yaoping Lv,&nbsp;Qingmin Dai,&nbsp;Ling Zhu,&nbsp;Siqi Liu,&nbsp;Zehui Hu,&nbsp;Junkai Lu,&nbsp;Haidong Zhou,&nbsp;Zufei Mei","doi":"10.1007/s10126-024-10335-7","DOIUrl":"10.1007/s10126-024-10335-7","url":null,"abstract":"<div><p>Naturally, the ovaries of many farmed fish can only develop to stage IV (mainly including stage IV oocytes, known as full-grown postvitellogenic oocytes). Therefore, spawn-inducing hormone injections are used to promote ovary development and oocyte maturation, facilitating reproduction in the aquaculture industry. The study of spawn-inducing hormones and their underlying neuroendocrine mechanisms has been a recent focus in fish reproductive biology. However, the intra-ovarian regulatory mechanisms of ovary development and oocyte maturation after hormone injection require further investigation. In this study, we explored the histological and transcriptomic map of the ovary of <i>Hemibarbus labeo</i> after hormone injection to reveal changes in the ovary. The gonad index significantly increased after hormone injection for 5.5 h, after which no significant change was observed. Histological analysis showed that the nuclei had moved to one side of the oocytes at 5.5 h after hormone injection. Moreover, the volume of the oocytes increased and their yolk membranes thickened. Oocytes then underwent their first meiotic division at 5.5–11 h after hormone injection. Subsequently, the follicular membrane was ruptured, and ovulation was completed at 11–16.5 h after hormone injection. In addition, we identified 3189 differentially expressed genes (DEGs) on comparing the transcriptomes at different time points after hormone injection. These DEGs were significantly enriched in the GO terms of nervous system process, molecular transducer activity, and extracellular region, and the KEGG pathways of TNF signaling and cytokine–cytokine receptor interaction; these may play important roles in ovary development and oocyte maturation. Within these pathways, genes such as <i>apoe</i>, <i>creb3</i>, <i>jun</i>, <i>junb</i>, <i>il11</i>, and <i>il8</i> may play important roles in steroid hormone synthesis and ovulation. Conclusively, our results show detailed sequential dynamics of oocyte development and provide new insights into the intra-ovarian regulatory mechanisms of ovarian development and oocyte maturation in <i>H. labeo</i>. These findings may be important for research on improving egg quality and reproduction in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"672 - 686"},"PeriodicalIF":2.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141441929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HcN57, A Novel Unusual Acidic Silk-Like Matrix Protein from Hyriopsis cumingii, Participates in Framework Construction and Nacre Nucleation During Nacreous Layer Formation","authors":"Can Jin,&nbsp;Fangmengjie Wei,&nbsp;Jiayi Zhang,&nbsp;Xiaoyang Tan,&nbsp;Taixia Fan,&nbsp;Wen Luo,&nbsp;Jiale Li","doi":"10.1007/s10126-024-10339-3","DOIUrl":"10.1007/s10126-024-10339-3","url":null,"abstract":"<div><p>In the classic molecular model of nacreous layer formation, unusual acidic matrix proteins rich in aspartic acid (Asp) residues are essential for nacre nucleation due to their great affinity for binding calcium. However, the acidic matrix proteins discovered in the nacreous layer so far have been weakly acidic with a high proportion of glutamate. In the present study, several silk-like matrix proteins, including the novel matrix protein HcN57, were identified in the ethylenediaminetetraacetic acid-soluble extracts of the nacreous layer of <i>Hyriopsis cumingii</i>. HcN57 is a highly repetitive protein that consists of a high proportion of alanine (Ala, 34.4%), glycine (Gly, 22.5%), and serine (Ser, 11.4%). It forms poly Ala blocks, Gly_nX repeats, an Ala-Gly repeat, and a Ser-Ala-rich region, exhibiting significant similarity to silk proteins found in spider species. The expression of HcN57 was specifically located in the dorsal epithelial cells of the mantle pallium and mantle center. Notably, expression of HcN57 was relatively high during nacreous layer regeneration and pearl nacre deposition, suggesting HcN57 is a silk matrix protein in the nacreous layer. Importantly, HcN57 also contains a certain content of Asp residues, making it an unusual acidic matrix protein present in the nacreous layer. These Asp residues are mainly distributed in three large hydrophilic acidic regions, which showed inhibitory activity against aragonite deposition and morphological regulation of calcite in vitro. Moreover, HcN57-dsRNA injection resulted in failure of nacre nucleation in vivo. Taken together, our results show that HcN57 is a bifunctional silk protein with poly Ala blocks and Gly-rich regions that serve as space fillers within the chitinous framework to prevent crystallization at unnecessary nucleation sites and Asp-rich regions that create a calcium ion supersaturated microenvironment for nucleation in the center of nacre tablets. These observations contribute to a better understanding of the mechanism by which silk proteins regulate framework construction and nacre nucleation during nacreous layer formation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"716 - 731"},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Responses and Larval-Stage Growth of Protandrous Yellowfin Seabream (Acanthopagrus Latus) to Different Polyethylene Microplastics Exposure","authors":"Meng Xu,&nbsp;Wenyu Fang,&nbsp;Genmei Lin,&nbsp;Xiaoshan Zhu,&nbsp;Jianguo Lu","doi":"10.1007/s10126-024-10334-8","DOIUrl":"10.1007/s10126-024-10334-8","url":null,"abstract":"<div><p>Polyethylene microplastics (PE-MPs) were widespread in the marine environment; thus, their influences on marine hermaphroditic fish cannot be ignored. This study intends to evaluate the adverse biological effects of two different sources of PE, identified by Raman spectroscopy, on protandrous yellowfin seabream (<i>Acanthopagrus latus</i>) larvae. Growth retardation, brain lesions, head/body length ratio increase, and neuroendocrine system disorders were found, and growth and neuroendocrine regulation-related genes such as <i>sstr2</i>, <i>ghrb</i>, <i>irs1</i>, UGT2B15, UGT2C1, <i>drd4a</i>, <i>esr2b, hsd3b7</i>, and <i>hsd17b2</i> were identified. PE microbeads (100 μm) showed more severe tissue damage on fish, while environmental PE fibers (500–2500 μm) showed more imperceptible adverse effects. There were 218 DEGs up-regulated and 147 DEGs down-regulated in the environmental PE group, while 1284 (up) and 1267 (down) DEGs were identified in the virgin PE group. PE-MP stress influenced physiological processes like growth and neuroendocrine regulation and cholesterol-steroid metabolism, and caused tissue damage in the fish larvae. The study highlights the effects of environmental PE exposure on hermaphroditic protandrous fish.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"931 - 942"},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Localization of Health-Promoting Peptides Derived from Fish Protein Hydrolyzates on Fish Muscle Proteins","authors":"Shugo Watabe,&nbsp;Nanami Mizusawa,&nbsp;Kenta Hosaka,&nbsp;Shoichiro Ishizaki,&nbsp;Lu Peng,&nbsp;Koji Nagata,&nbsp;Nobuhiko Ueki","doi":"10.1007/s10126-024-10331-x","DOIUrl":"10.1007/s10126-024-10331-x","url":null,"abstract":"<div><p>The four previously reported health-promoting dipeptides, valine-tyrosine, lysine-tryptophan, methionine-phenylalanine, and arginine-isoleucine, found in the fish muscle hydrolyzates, were mainly located in the myosin subfragment-1 heavy chain, whereas the health-promoting tripeptide, alanine-lysine-lysine, was found in the fibrous rod consisting of the myosin subfragment-2 and light meromyosin with a regular coiled-coil structure of α-helix, irrespective of the fish species. Furthermore, the localization of these peptides either in the random coil, β-sheet, or α-helix was also examined in the three-dimensional image, showing no specific tendency. Surprisingly, the same trend was observed even for the mammalian rabbit fast muscle myosin heavy chain. Since a trade-off between myofibrillar ATPase and structural stability has been reported for fish living at low environmental temperatures, it is speculated that fish muscle proteins, when ingested, are easily digested by various proteases in the human digestive tract and provide various health-promoting peptides also in vivo. While fish actin contained only two dipeptides, methionine-phenylalanine and valine-tyrosine, glyceraldehyde 3-phosphate dehydrogenase, one of the major components of fish muscle water-soluble protein, contained all of the four dipeptides and one tripeptide mentioned above.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"843 - 852"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accumulation and Phagocytosis of Fluorescently Visualized Macrophages Against Edwardsiella piscicida Infection in Established mpeg1.1-Transgenic Japanese Medaka Oryzias latipes","authors":"Juna Yamamoto,&nbsp;Hana Deguchi,&nbsp;Takechiyo Sumiyoshi,&nbsp;Kentaro Nakagami,&nbsp;Akatsuki Saito,&nbsp;Hiroshi Miyanishi,&nbsp;Masakazu Kondo,&nbsp;Tomoya Kono,&nbsp;Masahiro Sakai,&nbsp;Masato Kinoshita,&nbsp;Jun-ichi Hikima","doi":"10.1007/s10126-024-10333-9","DOIUrl":"10.1007/s10126-024-10333-9","url":null,"abstract":"<div><p>Intracellular bacteria such as those belonging to the genus <i>Edwardsiella</i> can survive and proliferate within macrophages. However, the detailed mechanisms underlying the host macrophage immune response and pathogen evasion strategies remain unknown. To advance the field of host macrophage research, we successfully established transgenic (Tg) Japanese medaka <i>Oryzias latipes</i> that possesses fluorescently visualized macrophages. As a macrophage marker, the macrophage-expressed gene 1.1 (<i>mpeg1.1</i>) was selected because of its predominant expression across various tissues in medaka. To validate the macrophage characteristics of the fluorescently labeled cells, May-Grünwald Giemsa staining and peroxidase staining were conducted. The labeled cells exhibited morphological features consistent with those of monocyte/macrophage-like cells and tested negative for peroxidase activity. Through co-localization studies, the fluorescently labeled cells co-localized with <i>E. piscicida</i> in the intestines and kidneys of infected medaka larvae, confirming the ingestion of bacteria through phagocytosis. In addition, the labeled cells expressed macrophage markers but lacked a neutrophil marker. These results suggested that the fluorescently labeled cells of Tg[<i>mpeg1.1</i>:mCherry/mAG] medaka were monocytes/macrophages, which will be useful for future studies aimed at understanding the mechanisms of macrophage-mediated bacterial infections.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"658 - 671"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification","authors":"Yuting Ma,&nbsp;Yongshuang Xiao,&nbsp;Zhizhong Xiao,&nbsp;Jun Li","doi":"10.1007/s10126-024-10336-6","DOIUrl":"10.1007/s10126-024-10336-6","url":null,"abstract":"<div><p>Spotted knifejaw (<i>Oplegnathus punctatus</i>) is a marine economic fish with high food and ecological value, and its growth process has obvious male and female sexual dimorphism, with males growing significantly faster than females. However, the current sex identification technology is not yet mature, which will limit the growth rate of <i>O. punctatus</i> aquaculture and the efficiency of separate sex breeding, so the development of efficient sex molecular markers is imperative. This study identified a 926 bp DNA insertion fragment in the <i>cdkn1</i>/<i>srsf3</i> intergenic region of <i>O. punctatus</i> males through whole-genome scanning, comparative genomics, and structural variant analysis. A pair of primers was designed based on the insertion information of the Y chromosome intergenic region in male individuals. Agarose gel electrophoresis revealed the amplification of two DNA fragments, 1118 bp and 192 bp, in male <i>O. punctatus</i> individuals. The 926 bp fragment was identified as the insertion in the intergenic region of <i>cdkn1</i>/<i>srsf3</i> in males, while only a single 192 bp DNA fragment was amplified in females. The biological sex of the individuals identified in this manner was consistent with their known phenotypic sex. In this study, we developed a method to detect DNA insertion variants in the intergenic region of <i>O. punctatus</i>. Additionally, we introduced a new DNA marker for the rapid identification of the sex of <i>O. punctatus</i>, which enhances detection efficiency. The text has important reference significance and application value in sex identification, all-male breeding, and lineage selection. It provides new insights into the regulation of variation in the intergenic region of <i>cdkn1</i>/<i>srsf3</i> genes and the study of RNA shearing.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"687 - 695"},"PeriodicalIF":2.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shotgun Metagenomics Reveals Taxonomic and Functional Patterns of the Microbiome Associated with Barbour’s Seahorse (Hippocampus barbouri)","authors":"Rose Chinly Mae H. Ortega-Kindica,&nbsp;Chinee S. Padasas-Adalla,&nbsp;Sharon Rose M. Tabugo,&nbsp;Joey Genevieve T. Martinez,&nbsp;Olive A. Amparado,&nbsp;Carlo Stephen O. Moneva,&nbsp;Rodelyn Dalayap,&nbsp;Carlos O. Lomeli-Ortega,&nbsp;Jose Luis Balcazar","doi":"10.1007/s10126-024-10330-y","DOIUrl":"10.1007/s10126-024-10330-y","url":null,"abstract":"<div><p>This study aimed to investigate the taxonomic and functional patterns of the microbiome associated with Barbour’s seahorse (<i>Hippocampus barbouri</i>) using a combination of shotgun metagenomics and bioinformatics. The analyses revealed that Pseudomonadota and Bacillota were the dominant phyla in the seahorse skin microbiome, whereas Pseudomonadota and, to a lesser extent, Bacillota and Bacteroidota were the dominant phyla in the seahorse gut microbiome. Several metabolic pathway categories were found to be enriched in the skin microbiome, including amino acid metabolism, carbohydrate metabolism, cofactor and vitamin metabolism, energy metabolism, nucleotide metabolism, as well as membrane transport, signal transduction, and cellular community-prokaryotes. In contrast, the gut microbiome exhibited enrichment in metabolic pathways associated with the metabolism of terpenoids and polyketides, biosynthesis of other secondary metabolites, xenobiotics biodegradation and metabolism, and quorum sensing. Additionally, although the relative abundance of bacteriocins in the skin and gut was slightly similar, notable differences were observed at the class level. Specifically, class I bacteriocins were found to be more abundant in the skin microbiome, whereas class III bacteriocins were more abundant in the gut microbiome. To the best of our knowledge, this study represents the first comprehensive examination of the taxonomic and functional patterns of the skin and gut microbiome in Barbour’s seahorse. These findings can greatly contribute to a deeper understanding of the seahorse-associated microbiome, which can play a pivotal role in predicting and controlling bacterial infections, thereby contributing to the success of aquaculture and health-promoting initiatives.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 4","pages":"835 - 841"},"PeriodicalIF":2.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}