Marine Biotechnology最新文献

{"title":"Biodegradation of Di-2-Ethylhexyl Phthalate by Mangrove Sediment Microbiome Impacted by Chronic Plastic Waste","authors":"Kanphorn Saeng-kla,&nbsp;Wuttichai Mhuantong,&nbsp;Teerasit Termsaithong,&nbsp;Onruthai Pinyakong,&nbsp;Prinpida Sonthiphand","doi":"10.1007/s10126-024-10399-5","DOIUrl":"10.1007/s10126-024-10399-5","url":null,"abstract":"<div><p>Plastic pollution through the leaching of di(2-ethylhexyl) phthalate (DEHP), a widely used plasticizer, has led to the emergence of mangrove pollution. This study aimed to assess the DEHP removal efficiency of indigenous mangrove sediment microbiomes and identify key DEHP degraders using microcosm construction and metagenomic analysis. During the 35-day incubation period, the indigenous mangrove sediment microbiome, affected by chronic plastic pollution, demonstrated a 99% degradation efficiency of 200 mg/kg DEHP. Spearman’s correlation analysis suggested that <i>Myxococcales</i>, <i>Methyloligellaceae</i>, <i>Mycobacterium</i>, and <i>Micromonospora</i> were potentially responsible for DEHP degradation. Based on PICRUSt2, the DEHP-degrading pathway in the sediment was predicted to be an anaerobic process involving catechol metabolism through <i>catC</i>, <i>pcaD</i>, <i>pcaI</i>, <i>pcaF</i>, and <i>fadA</i>. Efficient bacterial isolates from the mangrove sediment, identified as <i>Gordonia</i> sp. and <i>Gordonia polyisoprenivorans</i>, were able to degrade DEHP (65–97%) within 7 days and showed the ability to degrade other phthalate esters (PAEs).</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Reveals Molecular Mechanisms of Neuroendocrine Regulation of Allometric Growth in the Red Swamp Crayfish Procambarus clarkii","authors":"Zheyan Chen,&nbsp;Yongqing Wang,&nbsp;Xianji Tao,&nbsp;Yihai Qiao,&nbsp;Xilei Li,&nbsp;Jianbin Feng,&nbsp;Jiale Li","doi":"10.1007/s10126-024-10395-9","DOIUrl":"10.1007/s10126-024-10395-9","url":null,"abstract":"<div><p>Allometric growth is a typical characteristic of crustaceans, which mainly occurs among individuals, life stages, tissues, and between sexes. The red swamp crayfish <i>Procambarus clarkii</i> is an economically important crustacean species in the world. To date, the molecular regulatory mechanisms of neuroendocrine system in the allometric growth of <i>P. clarkii</i> remain unclear. In this study, <i>P. clarkii</i> exhibiting significant allometric growth among individuals were sampled from three full-sibling families. The brain, eyestalk, nerve cord, and Y-organ were dissected for transcriptome analysis. Key functional genes were identified by random forest and DESeq2 methods. The gene pathways were enriched utilizing Kyoto Encyclopedia Genes and Genomes (KEGG) analysis. Gene topological analysis was established through weighted gene co-expression network analysis (WGCNA), and hub genes were screened by protein–protein interaction (PPI) networks. Transcriptomic analysis results were validated via qRT-PCR. RNA-Seq identified 31 differentially expressed genes (DEGs) (7 up- and 24 downregulated); 301 DEGs (23 up- and 278 downregulated); 1308 DEGs (474 up- and 834 downregulated); and 64 DEGs (52 up- and 12 downregulated) in the brain, eyestalk, Y-organ, and nerve cord, respectively. Crucial functional genes such as <i>CHIA</i> in the brain and <i>perlucin-like</i> in the eyestalk were notably identified. WGCNA revealed two hub modules, while PPI networks identified neuroendocrine regulators module which hub genes mainly including <i>CP1876-like</i> and cuticle protein <i>AM1199-like</i>, and structural components module which hub genes mainly including <i>CUB&amp; CCP Domain-Containing Protein</i>, <i>ARRDC</i>, and <i>E3 Ubiquitin protein ligase MCYCBP2-like</i>. Correspondingly, the significant gene pathways such as amino sugar and nucleotide sugar metabolism (pcla00520) and insect hormone biosynthesis (pcla00981) were enriched. The results revealed the complex interactions and regulatory relationships of hub genes within hub modules to coordinate molting and growth. The results of RNA-Seq analysis were validated by the consistency of gene expression in qRT-PCR. In present study, key functional genes in the neuroendocrine system regulating allometric growth among individuals were identified, and significant pathways mainly include hormone synthesis were screened, thus constructing a neuroendocrine molecular regulatory network for the allometric growth of <i>P. clarkii</i>. Building on these investigations, a comprehensive mechanism whereby neuroendocrine regulators interact with structural components to coordinate molting and growth was proposed. The result would provide valuable insights into the molecular regulatory mechanisms of allometric growth, highlighting the interplay between the neuroendocrine system and relevant tissues.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Nile Tilapia Primary Cell Culture Methods and In Vitro Cell Knockdown Techniques","authors":"Siqi Huang,&nbsp;Shicen Wei,&nbsp;He Jiao,&nbsp;Songqian Huang,&nbsp;Qini Li,&nbsp;Zhe Wang,&nbsp;Yuhao Tang,&nbsp;Liangbiao Chen,&nbsp;Jigang Lu","doi":"10.1007/s10126-024-10380-2","DOIUrl":"10.1007/s10126-024-10380-2","url":null,"abstract":"<div><p>As an important aquaculture species and research model, Nile tilapia (<i>Oreochromis niloticus</i>) has not yet been systematically studied for the isolation, culture, and in vitro gene manipulation techniques of primary cells from various tissues. This study aimed to explore methods for isolating primary cells from various tissues, as well as developing in vitro gene manipulation techniques in Nile tilapia. Four different Nile tilapia tissues were enzymatically digested and separated using trypsin or collagenase. Collagenase (0.1%) was used for the digestion of the gonads, liver, and heart, while trypsin (0.25%) showed better adhesion efficiency for spleen tissue. Moreover, we assessed EGFP fluorescence intensity and cell survival rates following transfection with empty siRNA (siRNA-NC), lentivirus (LV-NC), and six adeno-associated virus (AAV-NC) serotypes (AAV2-NC, AAV5-NC, AAV6-NC, AAV8-NC, AAV9-NC, AAV-DJ-NC) in gonadal cells. The results demonstrated that cells transfected with siRNA-NC and LV-NC showed the highest levels of green fluorescent protein expression and survival rates in primary gonadal cells, compared to AAC-NC. Subsequently, we knocked down the <i>Kdm6bb</i> gene in Nile tilapia primary gonadal cells by transfecting them with LV-<i>Kdm6bb</i> and siRNA-<i>Kdm6bb</i>. qPCR and immunofluorescence analyses demonstrated a significant reduction in <i>Kdm6bb</i> mRNA levels following transfection with siRNA-<i>Kdm6bb</i> compared to siRNA-NC, and with LV-<i>Kdm6bb</i> compared to LV-NC. This study offers valuable tools for the validation of primary cell isolation and in vitro molecular regulatory mechanisms and functions in Nile tilapia.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Selenium Nanoparticles on Alternative Splicing of Rainbow Trout Head Kidney under Heat Stress","authors":"Jiahui Zhang,&nbsp;Zhe Liu,&nbsp;Jinqiang Quan,&nbsp;Junhao Lu,&nbsp;Guiyan Zhao,&nbsp;Yucai Pan","doi":"10.1007/s10126-024-10382-0","DOIUrl":"10.1007/s10126-024-10382-0","url":null,"abstract":"<div><p>Alternative splicing (AS) is an important post-transcriptional regulation, which can expand the functional diversity of gene products and is a mechanism for eukaryotes to cope with abiotic stress. However, there are few studies on AS events in rainbow trout under heat stress. In this study, RNA-Seq data were used to clarify the effect of selenium nanoparticles (SeNPs) on the AS events of rainbow trout head kidney under heat stress. The results showed that a total of 45,398 AS events were identified from 9804 genes, of which Skipped Exon (SE) was the most common type of AS event. Through the analysis of the differentially expressed genes (DEGs) in each group, we learned that DEGs were enriched in the spliceosome, and the relevant genes were significantly changed, which promoted the occurrence of AS. We found that lysine degradation, ubiquitin mediated proteolysis, RNA degradation, protein processing in endoplasmic reticulum processing and other pathways were significantly enriched after addition of SeNPs. In addition, some immune related signaling pathways, such as the mTOR signaling pathway, interact with each other to enhance the resistance of rainbow trout to heat stress. These results indicated that AS in head kidney of rainbow trout changed under heat stress and SeNPs played a key role in alleviating heat stress for rainbow trout.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals the lncRNA-mRNA Co-expression Network Regulating the Aestivation of Sea Cucumber","authors":"Xiaomei Chen,&nbsp;Wentao Han,&nbsp;Rui Yang,&nbsp;Xuan Zhu,&nbsp;Shengwen Li,&nbsp;Yangfan Wang,&nbsp;Xue Sun,&nbsp;Yuli Li,&nbsp;Lisui Bao,&nbsp;Lingling Zhang,&nbsp;Shi Wang,&nbsp;Jing Wang","doi":"10.1007/s10126-024-10388-8","DOIUrl":"10.1007/s10126-024-10388-8","url":null,"abstract":"<div><p>LncRNAs are long non-coding RNAs that are widely recognized as crucial regulators of gene expression and metabolic control, involved in numerous dormancy-related processes. Aestivation is a common hypometabolism strategy of sea cucumber (<i>Apostichopus japonicus</i>) in response to high-temperature conditions and is typically characterized by the degradation of the intestine and respiratory tree. Although the aestivation process has been extensively studied in sea cucumbers, the role of lncRNAs in the context of aestivation states remains a conspicuous knowledge gap. Here, we identified and characterized 14,711 lncRNAs in <i>A. japonicus</i> and analyzed their differential expression patterns during the aestivation process in the intestine and respiratory tree. The results revealed the physiological differences, especially the metabolic processes, between the intestine and respiratory tree during the aestivation. The co-expression network of lncRNA-mRNA suggested the dominant role of lncRNA in regulating the differential response of the intestine and respiratory trees. Differentially co-expressed factors were significantly enriched in the deep-aestivation stage-specific modules. Conserved co-expressed factors included several transcription factors known to be involved in rhythm regulation, such as <i>Klf2</i> and <i>Egr1</i>. Furthermore, a specific trans-acting lncRNA (<i>lncrna.1393.1</i>) was identified as a potential regulator of <i>Klf2</i> and <i>Egr1</i>. Overall, the systematic identification, characterization, and expression analysis of lncRNAs in <i>A. japonicus</i> enhanced our knowledge of long non-coding regulation of aestivation in sea cucumber and provided new clues for understanding the common “toolkit” of dormancy regulatory mechanisms.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Mapping of Autonomously Replicating Sequences in the Marine Diatom Phaeodactylum tricornutum","authors":"Hyun-Sik Yun,&nbsp;Kohei Yoneda,&nbsp;Takehito Sugasawa,&nbsp;Iwane Suzuki,&nbsp;Yoshiaki Maeda","doi":"10.1007/s10126-024-10390-0","DOIUrl":"10.1007/s10126-024-10390-0","url":null,"abstract":"<div><p>Autonomously replicating sequences (ARSs) are important accessories in episomal vectors that allow them to be replicated and stably maintained within transformants. Despite their importance, no information on ARSs in diatoms has been reported. Therefore, we attempted to identify ARS candidates in the model diatom, <i>Phaeodactylum tricornutum</i>, via chromatin immunoprecipitation sequencing. In this study, subunits of the origin recognition complex (ORC), ORC2 and ORC4, were used to screen for ARS candidates. ORC2 and ORC4 bound to 355 sites on the <i>P. tricornutum</i> genome, of which 69 were constantly screened after multiple attempts. The screened ARS candidates had an AT-richness of approximately 50% (44.39–52.92%) and did not have conserved sequences. In addition, ARS candidates were distributed randomly but had a dense distribution pattern at several sites. Their positions tended to overlap with those of the genetic region (73.91%). Compared to the ARSs of several other eukaryotic organisms, the characteristics of the screened ARS candidates are complex. Thus, our findings suggest that the diatom has a distinct and unique native ARSs.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory Genes in Eyespot Formation and Function of Mytilus coruscus","authors":"Minhui Xu,&nbsp;Jiji Li,&nbsp;Hongfei Li,&nbsp;Pengzhi Qi,&nbsp;Yingying Ye,&nbsp;Xiaojun Yan","doi":"10.1007/s10126-024-10396-8","DOIUrl":"10.1007/s10126-024-10396-8","url":null,"abstract":"<div><p>Light sensitivity is important for marine benthic invertebrates, and it plays a vital role in the marine bivalves settling. Animal visual systems are enormously diverse; their development appears to be controlled by a set of conserved retinal determination genes (RDGs). Eyespots, as the simplest animal eyes, their appearance indicates the important effect on mussel larvae attachment. Nevertheless, the molecular mechanism of the eyespot’s development in <i>Mytilus coruscus</i> larvae is not clear. In this study, we identified 11 genes which play a regulatory role in the visual system (i.e. <i>Pax1/9</i>, <i>Pax2/5/8</i>, <i>Pax6</i>, <i>Pax3/7</i>, <i>Six1/2, Six3/6</i>, <i>Six4/5</i>, <i>Dach</i>, <i>Eya</i>, <i>Brn</i> and <i>Tbx2</i>) from transcriptome data and the whole genome sequence of <i>M. coruscus</i>. The results of chromosome localization showed that 11 genes were distributed on different chromosomes. Subcellular mapping revealed that all the proteins except <i>Brn</i> were located in the nucleus. Phylogeny and gene structure analyses revealed that the <i>Pax</i> members were divided into four subfamilies, the <i>Six</i> members were divided into three subfamilies and structures within the same subfamily were relatively conserved. Quantitative real-time PCR (qPCR) showed that <i>Dach</i>, <i>Pax6</i>, <i>Pax3/7</i>, <i>Six1/2</i> and <i>Six4/5</i> were expressed at high levels during the pediveliger stage. Moreover, <i>Six1/2</i> and <i>Six4/5</i> were highly expressed in mantle tissues. Subsequent overall in situ hybridization experiments in the planktonic larval stage revealed that <i>Pax6</i>, <i>Six1/2</i> and <i>Six4/5</i> detected signals in the region of the eyespot. Based on these analyses, we suggested that the development of vision in <i>M. coruscus</i> not only depended on the expression pattern of <i>Pax6</i>, but perhaps also related to <i>Six1/2</i> and <i>Six4/5</i> in the planktonic larval stage, while <i>Six1/2</i> and <i>Six4/5</i> were the dominant genes for visual function in the adult mussel. This study made a comprehensive analysis of the visual function of <i>M. coruscus</i> at the genome level, which helps us to understand the intrinsic mechanism of the visual system of marine bivalves, and also provides a molecular basis for improving the attachment and metamorphosis rate of <i>M. coruscus</i> larvae.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Zinc Uptake Transporter ZIP1-II Is Involved in Zinc Accumulation in the Hepatopancreas of Pacific Oyster Crassostrea gigas","authors":"Ning Kong,&nbsp;Cong Luo,&nbsp;Mengjia Wang,&nbsp;Junyan Zhao,&nbsp;Xiang Li,&nbsp;Lingling Wang,&nbsp;Linsheng Song","doi":"10.1007/s10126-024-10379-9","DOIUrl":"10.1007/s10126-024-10379-9","url":null,"abstract":"<div><p>The Pacific oyster <i>Crassostrea gigas</i> is known to have an exceptional ability to accumulate zinc, which endows it with robust resistance to pathogens and makes it an excellent source of dietary zinc. ZIP1 has been identified as an important zinc uptake protein in other species, but its role in oysters remains unclear. In the present study, a ZIP1 homologue (<i>Cg</i>ZIP1-II) of the Zrt/Irt-like protein (ZIP) family was identified in <i>C</i>. <i>gigas</i>. The mRNA transcripts of <i>Cg</i>ZIP1-II were constitutively expressed in examined tissues of <i>C. gigas</i>, with higher levels in the hepatopancreas and gill. After zinc exposure, the mRNA transcripts of <i>Cg</i>ZIP1-II in the hepatopancreas showed a significant decline from 12 h to 14 d, while those in the gill significantly decreased at 72 h, followed by a recovery to basal level at 7 to 14 d. Immunocytochemical analysis revealed that the <i>Cg</i>ZIP1-II protein was mainly located at the plasma membrane of oyster hemocytes. Compared to the control cells, overexpression of <i>Cg</i>ZIP1-II in the transfected HEK293 cells resulted in a 2.44-fold (<i>p</i> &lt; 0.05) increase in zinc content after incubation with 100 μM zinc for 24 h. Inhibition of endogenous <i>Cg</i>ZIP1-II expression with siRNAs led to a 42% reduction in zinc content in the hepatopancreas of oysters. Similarly, in vivo blocking of <i>Cg</i>ZIP1-II with anti-<i>Cg</i>ZIP1-II antibody caused a 43% decrease in zinc content in the hepatopancreas. These results collectively indicated that <i>Cg</i>ZIP1-II functioned as a zinc uptake transporter in <i>C. gigas</i> and played a certain role in zinc accumulation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Key Genes Correlated with Economic Trait Superiorities and Their SNP Screening Through Transcriptome Comparisons, WGCNA and Pearson Correlation Coefficient in the Sea Cucumber Apostichopus Japonicus","authors":"Jingxian Sun,&nbsp;Haolin Wang,&nbsp;Yaoyao Zhan,&nbsp;Tanjun Zhao,&nbsp;Chengda Li,&nbsp;Cao Cheng,&nbsp;Zengdong Wang,&nbsp;Ange Zou,&nbsp;Yaqing Chang","doi":"10.1007/s10126-024-10384-y","DOIUrl":"10.1007/s10126-024-10384-y","url":null,"abstract":"<div><p>Variation in morphology-driven economic traits is a common issue hindering the development of the sea cucumber aquaculture industry. In this study, transcriptome comparisons, weighted gene correlation network analysis (WGCNA) and Pearson correlation coefficient (PCC) were first employed to identify key genes correlated with morphological variation in the sea cucumber <i>Apostichopus japonicus</i>, after which the relationship between identified key genes (relative expression and genotype) and economic trait phenotypes was investigated to screen potential biomarker targets for molecular-assisted breeding. The results showed that three genes (<i>putative ficolin–2</i>,<i> fibrinogen c domain–containing protein 1</i>, and <i>angiopoietin–4</i>) were closely associated with economic trait superiorities. Two single nucleotide polymorphisms (SNPs) were identified in the <i>putative ficolin–2</i> gene as having a strong correlation with body weight and papilla number. The findings from this study will enrich breeding biomarker resources and benefit the development of molecular-assisted breeding techniques in sea cucumber aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Read Sequencing Revealing the Effectiveness of Captive Breeding Strategy for Improving the Gut Microbiota of Spotted Seal (Phoca largha)","authors":"Jing Du,&nbsp;Zhen Wang,&nbsp;Yankuo Xing,&nbsp;Xianggang Gao,&nbsp;Zhichuang Lu,&nbsp;Duohui Li,&nbsp;Jiashen Tian","doi":"10.1007/s10126-024-10397-7","DOIUrl":"10.1007/s10126-024-10397-7","url":null,"abstract":"<div><p>The spotted seal (<i>Phoca largha</i>) is the sole pinniped species that can reproduce in China and has been classified as the First-Grade State Protection animal. The conventional method for the protection and maintenance of the spotted seal population is the captive maintenance of the species in artificially controlled environments. Nevertheless, the efficacy of the captive strategy remains uncertain, with the potential to impact the health of spotted seals through alterations in gut microbiota. In this study, PacBio sequencing based on the full-length of the bacterial 16S rRNA gene was applied to faeces from captive and wild spotted seals, thereby providing a first reference for the gut microbiota profile of spotted seals at the species scale. The gut microbiota of captive spotted seals was found to be more diverse than that of the wild population. The gut microbiota of spotted seals exhibited notable variation due to captive breeding, with an enrichment of Firmicutes and a reduction in Proteobacteria. The results of the co-occurrence network analysis indicated that the gut microbiota of captive spotted seals exhibited a greater degree of complexity and stability in comparison to that observed in their wild counterparts. The analysis of community assembly mechanisms revealed an increased determinism for the gut microbiota of captive individuals, with a concomitant decrease in the contribution of drift. Furthermore, the results of the predicted functions indicated a reduction in stress responses and an enhanced ability to metabolise sugars in the gut microbiota of captive spotted seals. In conclusion, the results of this study provide evidence that the current captive breeding strategy is an effective approach for improving the gut microbiota of spotted seals. Furthermore, this study demonstrates the potential of monitoring the gut microbiota to assess the health of marine mammals and inform conservation strategies for endangered species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}