Marine Biotechnology最新文献

{"title":"Effects of Environmentally Friendly Aquaculture Chamber Coatings on Enzyme Activities, Histology, and Transcriptome in the Liver of Larimichthys crocea","authors":"Xuan Xu,&nbsp;Huayu Song,&nbsp;Huicai Wu,&nbsp;Lu Zhang,&nbsp;Fengjun Lin,&nbsp;Chonghui Chen,&nbsp;Xiaoxu Zhang,&nbsp;Yiying Liu,&nbsp;Chao Li,&nbsp;Qiang Fu","doi":"10.1007/s10126-025-10453-w","DOIUrl":"10.1007/s10126-025-10453-w","url":null,"abstract":"<div><p>Aquaculture vessels have emerged as a sustainable alternative to traditional offshore aquaculture. However, the biological impacts of protective coatings used for vessel interiors are still poorly understood. This study assessed acute stress responses of <i>Larimichthys crocea</i> to epoxy-based aquaculture coatings using actual culture (1-fold) and high-exposure (80-fold) concentrations. Liver analyses included antioxidant enzymes, histopathology, and transcriptomics over 12–96 h. Firstly, the effect of the 80-fold concentration group on the activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) was more significant in the liver of <i>L. crocea</i> compared to the 1-fold concentration group. Similarly, histological observations revealed that the 80-fold concentration group produced more significant pathological changes in the liver than the 1-fold concentration group, including hepatocyte damage and vacuolization. Subsequently, through high-throughput sequencing, a total of 714.02 million clean reads were obtained, with 693.71 million of these reads successfully mapped onto the reference genome of <i>L. crocea</i>, identifying 13,709 differentially expressed genes (DEGs). KEGG pathway enrichment analysis showed that many DEGs following coating-treated were involved in protein processing in endoplasmic reticulum, oxidative phosphorylation, cytokine-cytokine receptor interaction, FoxO signaling pathway, and toll-like receptor signaling pathway. Finally, fifteen DEGs were selected for quantitative real-time PCR (qRT-PCR) analysis, and the results showed a significant correlation with RNA-seq results, verifying the reliability and accuracy of the high-throughput sequencing data. This study preliminarily revealed the stress responses induced by aquaculture vessel coatings in <i>L. crocea</i> and provided fundamental data into the scientific use of coatings on aquaculture vessels.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological and miRNA-mRNA Analyses Provide New Insights into the Immune Response of Clams to Vibrio Infection","authors":"Jianxin Jia,&nbsp;Hongtao Nie","doi":"10.1007/s10126-025-10454-9","DOIUrl":"10.1007/s10126-025-10454-9","url":null,"abstract":"<div><p>Manila clam plays a crucial role in China’s marine aquaculture industry. However, frequent vibriosis outbreaks severely hinder sustainable and healthy development of the shellfish aquaculture industry. This study indicated markedly decreased clam survival rates after 48 h of <i>Vibrio alginolyticus</i> challenge. Gill and hepatopancreas damage was investigated through histological observation. The activity of lysozyme in the gills and hepatopancreas peaked at 12 and 24 h, respectively. <i>V. alginolyticus</i> showed a maximum bacterial load in the gills and hepatopancreas at 12 and 24 h, respectively. Additionally, transcriptome sequencing of hepatopancreas revealed ten differentially expressed miRNAs in Va and Cn after 48 h infection with <i>V. alginolyticus</i>, corresponding to 100 target genes, with eight upregulated and two downregulated DE miRNAs. Gene ontology (GO) enrichment analysis identified 50 known miRNAs and 111 novel miRNAs, thereby predicting a total of 1840 target genes. KEGG analysis revealed significant changes in multiple signaling pathways, involving lysosomes, apoptosis, amino acid metabolism, and endocytosis, in response to <i>V. alginolyticus</i> stimulation. This study provided new information regarding the immune regulation mechanisms of <i>R. philippinarum</i> in response to <i>V. alginolyticus</i> stress.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-(1 → 3) Linked Sulfated Polygalactan from a Seaweed-associated Bacillus velezensis MTCC 13097: A Potential Lead Against Human Hepatocellular Adenocarcinoma","authors":"Sumayya Asharaf,&nbsp;Kajal Chakraborty,&nbsp;Silpa Kunnappilly Paulose,&nbsp;Chesvin Varghese,&nbsp;Shubhajit Dhara","doi":"10.1007/s10126-025-10447-8","DOIUrl":"10.1007/s10126-025-10447-8","url":null,"abstract":"<div><p>Among tumors, liver cancer has an inferior prognosis. Therefore, exploring alternative strategies to improve the effectiveness of treatment for this ailment is of utmost urgency. In this study, we focused on analyzing the anti-cancer properties of bacterial exopolysaccharide from <i>Bacillus velezensis</i> associated with the seaweed <i>Sargassum wightii</i> against hepatocellular adenocarcinoma. A culture-dependent method was used to isolate heterotrophic <i>B. velezensis,</i> which was then evaluated for its antioxidant and anti-cancer properties. A <i>β</i>-(1 → 3) linked sulfated polygalactan exopolysaccharide (BVEP-2) was isolated from the bacterial extract and characterized by spectroscopic analysis. The anti-cancer property was analyzed through assays involving 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red uptake (NRU), and apoptosis (by annexin V-FITC/PI staining) against the human hepatocellular adenocarcinoma cell line (HepG2). BVEP-2 demonstrated potential cytotoxicity in the MTT assay (IC₅₀ 65.05 μg/mL) and 23.02% cell viability in the NRU assay at a 100 µg/mL concentration of BVEP-2 against HepG2, compared to the standard doxorubicin. Potential antioxidant properties of BVEP-2 (IC₅₀ 112–117 µg/mL) corroborated the anti-cancer activities, and the attenuation of free radicals could play a significant role in its anti-cancer potential. BVEP-2 induced approximately 9% early apoptosis and 39% late apoptosis in the HepG2 cell line, whereas the standard drug resulted in around 38% early apoptosis and 37% late apoptosis, along with 6% necrotic cells. The <i>β</i>-(1 → 3) linked sulfated polygalactan exopolysaccharide (BVEP-2) of <i>B. velezensis</i> MTCC13097 showed potential antioxidant and anti-cancer activities, and thus, could be developed as a promising pharmacophore lead against human hepatocellular adenocarcinoma.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Compounds Derived from Marine Halophilic Bacteria: Studies on In Vitro and In Silico Docking","authors":"Arul Dhayalan,&nbsp;Balasubramani Govindasamy,&nbsp;Ashwini Ramesh,&nbsp;Suvithra Sakkarabani,&nbsp;Abdulwahed Fahad Alrefaei,&nbsp;Muthu Thiruvengadam,&nbsp;Deepak Paramasivam","doi":"10.1007/s10126-025-10450-z","DOIUrl":"10.1007/s10126-025-10450-z","url":null,"abstract":"<div><p>Halophilic marine bacteria have been considered for their potential secondary metabolites that possess antagonistic activity, bio-reducing activity, and a number of clinical and probiotic applications. In total, 12 halophilic bacterial strains were isolated from marine water and soil sediment samples. From the primary antimicrobial screening of the cell-free supernatant of all isolates, T7 and N5 strains showed significant results. The genomic DNA of these strains was isolated and their nucleotide sequencing confirmed as <i>Vibrio alginolyticus</i> (T7) and <i>Aeromonas veronii</i> (N5), respectively. The bacterial secondary metabolites from ethyl acetate extract (EAE) were tested against pathogens (<i>Vibrio harveyi</i>, <i>Vibrio parahaemolyticus</i>, <i>Vibrio</i> spp., and <i>Aeromonas hydrophila</i>). The Fourier transform infrared (FT-IR) characterization of the EAE revealed nine functional groups, primarily alkanes and amides, in each isolate (T7 and N5), while gas chromatography mass spectrometry (GC–MS) identified nine compounds from <i>V. alginolyticus</i> (T7) and four from <i>A. veronii</i> (N5). The molecular docking was performed for 12 compounds against proteins from pathogens: DNA gyrase subunit B and the topoisomerase IV (Topo IV), which revealed the potential binding mode of the ligands to the sites of the appropriate targets. Whereas the compound decyl phthalate from <i>V. alginolyticus</i> showed an active binding affinity value − 5.5 to − 6.6 kcal/mol against gyrB and Topo IV proteins of pathogens, compounds phthalic acid, 2-ethylhexyl pentadecyl ester, and bis(2-ethylhexyl) phthalate from <i>A. veronii</i> showed high binding affinity values of − 6.1 to − 7.2 and − 5.7 to − 6.8 kcal/mol against fish pathogen proteins of the gyrB protein and Topo IV, respectively. Thus, the protein with ligand binding affinity indicates a stronger inhibition of <i>V. harveyi</i> and <i>A. hydrophila</i> compared to other pathogens. Overall, the compounds from the EAE of <i>A. veronii</i> were found to be highly effective against pathogens compared to the EAE of <i>V. alginolyticus</i>. Therefore, further studies are needed to explore the potential therapeutic applications in treating bacterial infections in aquaculture settings.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpenoids with Antiangiogenic Activity from Formosan Soft Coral Caementabunda hypotentaculata","authors":"Hsin Huang,&nbsp;Chen-Yu Yang,&nbsp;Shih-Wei Wang,&nbsp;Limbadri Salendra,&nbsp;Shu-Rong Chen,&nbsp;Tzi-Yi Ke,&nbsp;Yu-Liang Yang,&nbsp;Yu-Chi Lin,&nbsp;Yuan-Bin Cheng","doi":"10.1007/s10126-025-10452-x","DOIUrl":"10.1007/s10126-025-10452-x","url":null,"abstract":"<div><p>Soft corals of the genus <i>Caementabunda</i> (synonym <i>Cespitularia</i>) were reported to possess terpenoids with various carbon skeletons. In this research, samples of <i>C. hypotentaculata</i> were collected before and after spawning (in May and August) to compare their secondary metabolites. With the aid of LC–MS/MS molecule networking, a series of unknown verticillane-type diterpenoids were targeted. After repeated column chromatography, five new verticillane-type diterpenoids (<b>1</b>‒<b>5</b>) and one new eudesmanolide-type sesquiterpenoid (<b>6</b>), together with twenty-four known compounds (<b>7</b>‒<b>30</b>), were identified from the Formosan soft coral <i>C. hypotentaculata</i>. Their structures were determined by UV, IR, MS, and NMR analyses, together with the biogenetic considerations of terpenoids. Our pharmacological study showed that the ethanol extract of <i>C. hypotentaculata</i> exhibited antiangiogenic potential against human endothelial progenitor cells. Among all isolated compounds, compound <b>30</b> demonstrated a moderate antiangiogenic effect with an IC₅₀ value of 29.0 ± 0.6 μM.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type I Iodothyronine Deiodinase Functions in Thyroid Hormone Synthesis and Growth Regulation in Bivalve","authors":"Xiangchao Zhang,&nbsp;Yuli Niu,&nbsp;Qianqian Zhao,&nbsp;Ruoxi Wang,&nbsp;Xiangfu Kong,&nbsp;Xiaoli Hu,&nbsp;Zhenmin Bao","doi":"10.1007/s10126-025-10451-y","DOIUrl":"10.1007/s10126-025-10451-y","url":null,"abstract":"<div><p>Bivalve breeding programs account for over 50% of global marine aquaculture, and growth is one of the most important traits of them. With the purpose of investigating the key genes related to growth performance of Yesso scallop (<i>Patinopecten yessoensis</i>), an economically important bivalve, we conducted transcriptome comparison between fast- and slow-growing individuals, and identified 1,531 differentially expressed genes (DEGs), with 656 up-regulated in fast-growing scallops, categorizing into six molecular function terms, of which the thyroxine 5’-deiodinase activity term exhibited the lowest <i>p</i> values. This term contained only two genes, and they were both type I iodothyronine deiodinase (<i>D1</i>), the enzyme catalyzing triiodothyronine (T₃) production in vertebrates. Meanwhile, T₃ concentration was significantly higher in fast-growing scallops. To further understand <i>D1</i> functions in bivalve growth, RNA interference (RNAi) was performed in the dwarf surf clam (<i>Mulinia lateralis</i>), a suitable bivalve for verifying the function of growth-related genes. Suppression of <i>MlD1-1</i> and <i>MlD1-2</i> expression was detected after RNAi, and in <i>MlD1-1</i> RNAi group, dwarf surf clams exhibited significant reductions in growth, with shell length, shell width, shell height, and body weight decreasing by 9%, 9%, 9%, and 25%, respectively. Furthermore, the triiodothyronine (T₃) and thyroxine (T₄) levels of clams in <i>MlD1-1</i> RNAi group were significantly decreased by 11% and increased by 5%, respectively, while no changes were observed in the <i>MlD1-2</i> RNAi group. Our data imply that bivalve <i>D1</i> could be a candidate gene for the breeding of fast-growing bivalve.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin-3-gallate Attenuates the Bromo-3-chloro-5,5-dimethylhydantoin-induced Immunotoxicity in Crayfish","authors":"Yong Wang,&nbsp;Yinglei Xu,&nbsp;Fei Zhu","doi":"10.1007/s10126-025-10449-6","DOIUrl":"10.1007/s10126-025-10449-6","url":null,"abstract":"<div><p>Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH), a widely used environmental disinfectant in aquaculture, may induce toxicity, adversely affecting the health and viability of aquatic organisms. Epigallocatechin-3-gallate (EGCG), a polyphenol present in green tea, exhibits antioxidant properties that can protect normal cells from oxidative stress. The findings suggest that exposure to BCDMH results in a reduction of antioxidant enzyme activity, whereas EGCG supplementation enhances crayfish immunity and alleviates damage. Moreover, BCDMH exposure is associated with a decrease in total hemocyte count and an increase in apoptosis rate; however, EGCG demonstrates a protective effect against BCDMH-induced cytotoxicity. Histopathological analysis indicates that exposure to BCDMH results in hepatopancreatic damage in crayfish, which is mitigated by EGCG. To identify the genes and pathways influenced by EGCG, a comparative transcriptome analysis was performed. Gene Ontology (GO) analysis revealed that proteolysis and innate immune response are significant biological processes induced by EGCG. Furthermore, KEGG pathway analysis identified endocytosis and phagosome as critical pathways modulated by EGCG. EGCG effectively enhanced the survival of crayfish challenged with <i>V. alginolyticus</i> following BCDMH exposure This study contributes to fully understand the mechanisms of EGCG in reducing the immunotoxicity of antibiotic residues on aquatic animals.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Metabolomic Analyses Reveal Response Mechanisms of Sinonovacula Constricta to Saline-Alkalinity Stresses","authors":"Min Yang,&nbsp;Yuting Han,&nbsp;Yujie Chang,&nbsp;Chengbo Li,&nbsp;Donghong Niu","doi":"10.1007/s10126-025-10445-w","DOIUrl":"10.1007/s10126-025-10445-w","url":null,"abstract":"<div><p>The razor clam (<i>Sinonovacula constricta</i>) is a key species in marine aquaculture, known for its wide salinity adaptation, and potential for cultivation in saline-alkaline water. Understanding its response mechanisms is crucial for expanding its farming into these regions. This study reveals the response mechanisms of <i>S. constricta</i> in response to low-salinity alkaline stress through a combined analysis of transcriptomics and metabolomics. After 24 h of salt-alkali stress (SA group), 1378 differentially expressed genes (DEGs) were identified, with enriched pathways including glycerophospholipid metabolism, serine, taurine, and hypotaurine metabolism. Additionally, 341 significantly different metabolites (SDMs) were found, primarily involved in taurine and hypotaurine metabolism, purine metabolism, and the FoxO signaling pathway, etc. Both DEGs and SDMs were notably enriched in hypotaurine metabolism, glycerophospholipid metabolism, and the mTOR signaling pathway, showing significant upregulation in the SA group. Correlation analysis found that the integrated regulatory network was involved in the synthesis of taurine, glycerophospholipids, and L-glutamic acid, and the metabolism of 3-mercaptopropionic acid. These results suggest that low salinity and alkalinity induce stress responses in <i>S. constricta</i> by regulating osmotic balance, phospholipid synthesis, and lipid metabolism. This study offers insights into the molecular mechanisms of salt-alkali response in <i>S. constricta</i>. </p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Plastid Acetyl-CoA Carboxylase Confers Stress Tolerances with Increased Levels of Unsaturated Fatty Acids in the Marine Diatom Phaeodactylum tricornutum","authors":"Masakazu Toyoshima,&nbsp;Hajime Okuda,&nbsp;Yuya Okada,&nbsp;Kohei Yoneda,&nbsp;Ginga Shimakawa,&nbsp;Yusuke Matsuda","doi":"10.1007/s10126-025-10446-9","DOIUrl":"10.1007/s10126-025-10446-9","url":null,"abstract":"<div><p>Acetyl-coenzyme A carboxylases (ACCs) catalyze the initial reaction of fatty acid (FA) biosynthesis. The marine diatom <i>Phaeodactylum tricornutum</i> has two nuclear-encoded ACCs (PtACC1 (Phatr3_EG01955) and PtACC2 (Phatr3_J55209)), both which are homomeric and predicted to be localized in the plastids and the cytosol, respectively. In this study, we focused on stromal ACC1 by constructing <i>P. tricornutum</i> strains expressing GFP-tagged PtACC1 (ACCG strains) and confirmed that PtACC1 was localized in or around the pyrenoid. Here, we showed that unsaturated FAs (UFAs) composing the thylakoid membrane lipids increased in PtACC1 strains grown under high light conditions (190 µmol photons m⁻² s⁻¹), and that the content of triacylglycerol (TAG) and unsaturation ratios in TAG increased under oxidative stresses (with added 50 µM H₂O₂). ACCG strains showed faster growth rates than wild type under high light and/or oxidative stress conditions. These results suggest that cell proliferation is maintained by an accelerated recovery of PSII due to the increased UFAs in the thylakoid membrane in ACCG strains grown in high light, and that increased UFAs in ACCG cells enhanced the tolerance to oxidative stresses presumably due to the increased scavenging capacity of UFAs against reactive oxygen species. The introduction of plastidic ACC resulted in stimulating supply of UFAs to specific lipids that in turn enhanced tolerance to various stresses.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10446-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals Differential Gene Regulation in Ovarian Tissues of Anabas testudineus (Bloch, 1792) in Response to Insecticide, Monocrotophos","authors":"Siyag A. Dhere,&nbsp;Pokanti Vinay Kumar,&nbsp;Kiran D. Rasal,&nbsp;Sujata Mohapatra,&nbsp;Prachi Asgolkar,&nbsp;Manohar Vasam,&nbsp;Sheetal Vanjre,&nbsp;Priyanka S. S.,&nbsp;Manoj P. Brahmane,&nbsp;Arvind Sonwane,&nbsp;Rajesh Kumar,&nbsp;Naresh S. Nagpure,&nbsp;Mukunda Goswami,&nbsp;Jitendra Sundaray","doi":"10.1007/s10126-025-10443-y","DOIUrl":"10.1007/s10126-025-10443-y","url":null,"abstract":"<div><p>Monocrotophos (MCP), an organophosphate insecticide commonly used in agriculture, has raised concerns due to its runoff into aquatic ecosystems and causes potential adverse effects on fish. The present study envisaged the understanding of the impact of MCP on the ovarian tissues of <i>Anabas testudineus</i> (climbing perch), an air-breathing food fish often found close to agricultural fields, making it a valuable bio-indicator of agrochemical contamination. Transcriptome profiling of ovarian tissues in response to 45 days of MCP exposure at sub-lethal concentrations was performed. Using Illumina platform sequencing, a total of 144.51 million reads were produced. After filtering and trimming, 138.82 million high-quality reads were obtained, of which 96.10% were mapped to the Anabas genome. Expression analysis revealed a total of 54 significant differentially expressed genes (DEGs), including 28 upregulated genes, and 26 downregulated genes compared to the control group (Log2 FC &gt;  ± 1 and, adjusted <i>p-value</i> &lt; 0.05). Gene ontology analysis of the DEGs revealed associations with molecular, biological, and cellular functions. Key detoxification genes, such as <i>glutathione S-transferase</i> and <i>UDP-glucuronosyltransferase</i>, were significantly upregulated, indicating an enhanced detoxification response to MCP. In contrast, <i>cytochrome P450 family 1 subfamily A (cyp1a1)</i>, a gene critical for steroid hormone metabolism, was downregulated, suggesting disruptions in hormone regulation. Functional enrichment analysis highlighted several affected processes, including steroid hormone biosynthesis, oocyte meiosis, apoptosis, and progesterone-mediated oocyte maturation. The randomly selected eight DEGs using RT-qPCR confirmed consistent gene expression levels in line with the transcriptome data. This work identified significant genes associated with detoxification and reproduction events in the ovarian tissues for maintaining homeostasis. This will also serve as valuable information for further investigation of the association of the identified genes with the reproductive biology of fish in response to toxicants or pollutants.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}