Marine Biotechnology最新文献

{"title":"Analysis of Striped Bass (Morone saxatilis) and White Bass (M. chrysops) Splenic Transcriptome Following Streptococcus iniae Infection","authors":"Linnea K. Andersen,&nbsp;Jason W. Abernathy,&nbsp;Bradley D. Farmer,&nbsp;Miles D. Lange,&nbsp;Nithin M. Sankappa,&nbsp;Matthew E. McEntire,&nbsp;Steven D. Rawles","doi":"10.1007/s10126-025-10431-2","DOIUrl":"10.1007/s10126-025-10431-2","url":null,"abstract":"<div><p>Streptococcal disease results in major mortality events of both marine and freshwater fishes worldwide. <i>Streptococcus iniae</i> is among the prominent causative bacterial strains as it has been found to cause a higher incidence of mortality and act as a zoonotic pathogen. Here, we examine the susceptibility of two important aquaculture species in the USA, striped bass (<i>Morone saxatilis</i>) and white bass (<i>Morone chrysops</i>) to <i>S. iniae</i>. A high incidence of mortality was observed in both species, although striped bass succumbed more rapidly than white bass. Spleen gene expression at three time points following infection was analyzed to further elucidate the mechanisms underlying these observations. The down-regulation of gene transcripts associated with pathogen detection (<i>tlr1</i>, <i>tlr8</i>, <i>tlr9</i>), antigen processing (<i>cd74a</i>), immune cell recruitment and migration (<i>ccr6b</i>, <i>ccr7</i>), macrophage function (<i>csf1ra</i>), T-cell signaling, and NF-kB activation (<i>card11</i>, <i>fyna</i>, <i>tirap</i>) was detected in both species. These findings potentially indicate impairment in these critical early immune system processes such that both species were ultimately highly susceptible to <i>S. iniae</i> infection despite the detected up-regulation of transcripts typically associated with effective immune response, such as cytokines (<i>il1β</i>, <i>il8</i>, <i>il12b2</i>, <i>il17rc</i>, <i>tnfα</i>) and hepcidins (<i>hamp</i>, <i>hamp2</i>). The presented results collectively identify several candidate genes and associated pathways for further investigation to characterize the vulnerability of striped bass and white bass to <i>S. iniae</i> and that may be considered for selective breeding efforts, biotechnological intervention, and/or exploitation in the development of vaccines and alternative treatments.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid- and Multivariate-Based Analyses to Determine Cell Response to pH Variations and Buffer Composition","authors":"Ricardo F. S. Pereira,&nbsp;Carla C. C. R. de Carvalho","doi":"10.1007/s10126-025-10421-4","DOIUrl":"10.1007/s10126-025-10421-4","url":null,"abstract":"<div><p>During marine bioprocess development, pH control is of paramount importance. In shake flasks, aerobic fermentations usually have no pH control except from the buffering effect provided by buffers in the medium. However, the use of traditional buffers, such as phosphate buffer, can lead to the precipitation of medium components. Good’s buffers may be a sound alternative. Using <i>Serratia rubidaea</i> cells and their production of prodigiosin as model system, several Good’s buffers were tested and compared to phosphate buffer. Lipidomics analysis in conjugation with statistical multivariate analysis was performed to determine the cellular response to pH variations and buffer composition. Biomass productivity was similar when using the different buffers, but prodigiosin production was highly influenced and was highest with MES buffer at pH 5.5, reaching 249.8 mg/L, which corresponds to 43.7 mg/g_biomass. At pH 7.0, the best results were achieved with EPPS, HEPES and TRIS buffer, being these good substitutes for phosphate buffer in marine medium. The results also show that cells adapted the fatty acid composition of their membranes as response to the buffering species present in the growth medium. This is a clear indication that the buffer composition should not be disregarded when developing a bioprocess.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10421-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Efficient Method for Enrichment and In Vitro Propagation of Muscle Stem Cells Derived from Black Sea Bream (Acanthopagrus schlegelii) Skeletal Muscle","authors":"Dan Hee Han,&nbsp;Seung Pyo Gong","doi":"10.1007/s10126-025-10428-x","DOIUrl":"10.1007/s10126-025-10428-x","url":null,"abstract":"<div><p>Muscle stem cells (MSCs) play a crucial role in muscle growth, repair, and regeneration, offering potential applications in cell-mediated therapy, tissue engineering, and alternative food production. Despite significant advancements in isolating and enriching MSCs from mammalian tissues, research on fish MSCs remains limited. This study aimed to establish an optimized protocol for isolating, enriching, and propagating black sea bream (<i>Acanthopagrus schlegelii</i>) MSCs for potential biotechnological applications. Skeletal muscle tissues were enzymatically dissociated using various enzymes, with collagenase type II and pronase identified as the most effective combination for cell isolation and tissue debris removal. Differential plating (DP) on collagen type I effectively enriched MSCs, as evidenced by a significant increase in <i>Pax7</i> expression in non-adhesive cells. Among several adhesion substrates tested, Matrigel-coated dishes best supported the maintenance and differentiation potential of enriched MSCs, enabling robust myotube formation. To mitigate the high cost of Matrigel, cells were transitioned to laminin- or gelatin-coated dishes after the early passages. Notably, Matrigel-conditioned cells maintained their survival and differentiation capacities on these more cost-effective substrates. After long-term culture on gelatin-coated dishes, the cell lines were stably maintained for more than 25 passages, and their myogenic differentiation potentials were well preserved, with variations observed between the cell lines. These findings provide a foundational framework for the efficient isolation, enrichment, and culture of fish MSCs, contributing to the development of scalable and cost-effective protocols for their application in muscle biology and biotechnology.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Benefits of Dietary Selenium Nanoparticles (SeNPs) in Asian Seabass (Lates calcarifer) Male Broodstock","authors":"Saeed Keyvanshokooh,&nbsp;Amir Parviz Salati,&nbsp;Ahmad Ghasemi,&nbsp;Samira Nazemroaya,&nbsp;Hossein Houshmand,&nbsp;Mansour Torfi Mozanzadeh","doi":"10.1007/s10126-025-10429-w","DOIUrl":"10.1007/s10126-025-10429-w","url":null,"abstract":"<div><p>Selenium (Se), a critical trace element for human and animal health, is essential for numerous physiological processes, including antioxidant defense, immune function, and reproduction. This study investigated the effects of dietary selenium nanoparticles (SeNPs) at 2 mg/kg on the reproductive performance, Se deposition, antioxidant status, and gene expression in male Asian seabass (<i>Lates calcarifer</i>). Over 120 days before spawning, the male broodfish were fed either a control diet (CD) or a SeNP-supplemented diet (SeD) in triplicate tanks per group. The SeD group exhibited significantly higher gonadosomatic index (GSI), fertilization rate, and hatching rate, along with lower rates of abnormal embryogenesis compared to the CD group (<i>P</i> &lt; 0.05). Selenium accumulation was significantly elevated in the liver and testis tissues of the SeD group, corresponding with increased expression of the hepatic <i>selenop</i> gene. Reproduction-related genes, including <i>ar</i>, <i>p450scc</i>, and <i>cdk1</i>, were significantly upregulated in the testis of the SeD group, indicating enhanced reproductive processes. Antioxidant-related genes, including catalase (<i>cat</i>), superoxide dismutase (<i>sod</i>), and glutathione-s-transferase (<i>gst</i>) were also upregulated in both liver and testis tissues, while catalase activity increased in the testis. In terms of sex steroid hormones, the SeD group displayed significantly higher serum levels of testosterone and progesterone (<i>P</i> &lt; 0.05), suggesting enhanced steroidogenesis. The absence of adverse effects further demonstrates the efficacy and safety of SeNP supplementation at 2 mg/kg. This study highlights the potential of SeNPs to enhance reproductive performance, regulate molecular pathways, and optimize broodstock management in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Chromatin Accessibility and Transcriptional Regulation in the Eyes of Red Tilapia (Oreochromis sp.) in Response to Wintering Stress","authors":"Ying Bai,&nbsp;Gan Yang,&nbsp;Tongde Liu,&nbsp;Fuyan Chen,&nbsp;Junhong Xia","doi":"10.1007/s10126-025-10424-1","DOIUrl":"10.1007/s10126-025-10424-1","url":null,"abstract":"<div><p>During wintering, red tilapia may develop variable black spots on their bodies, significantly reducing their market value. Understanding the mechanisms driving this phenomenon is essential for molecular improvements in body color. In this study, we investigated chromatin accessibility landscapes in the eyes of red tilapia with two distinct phenotypes (normal pure red and black spot) under wintering stress using ATAC-seq and RNA-seq analyses. We observed that approximately 32.7% of chromatin accessibility peaks were located in promoter regions, followed by intergenic regions (32.4%) and intronic regions (26.7%). One thousand two hundred twenty-nine differentially accessible regions (DARs) and 1448 differentially expressed genes (DEGs) were identified between the RS and DS groups. Notably, DEGs associated with melanin synthesis, including <i>tyrp1</i>, <i>tyr</i>, <i>tyrp1b</i>, <i>pmela</i>, <i>slc24a5</i>, and <i>mlph</i>, were significantly upregulated in the DS group, which aligns with the observed 1.85-fold increase in melanin content, compared to the RS group. 92 DEGs were associated with significant changes in chromatin accessibility between groups (<i>R</i>² = 0.8059; <i>p</i> &lt; 0.0001), indicating potential regulatory relationships. Interestingly, 23.92% of the DARs were located on the chromosome 3. Specifically, a 2.5-fold difference in average peak height on LG3: 11,215,273–11,217,225 were observed between DS and RS tilapia. In the region, transcription factors including HSF1 and HSF2 were identified as key regulators of chromatin structure and gene expression under wintering stress. Our findings reveal that dynamic chromatin accessibility in the eyes of red tilapia facilitates adaptation to wintering stress by regulating visual signaling, melanin production, and downstream pigmentation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CgPPOX and CgFECH Mediate Protoporphyrin IX Accumulation Predominantly in the Outer Mantle Fold of Pacific Oyster Crassostrea gigas","authors":"Biyang Hu,&nbsp;Hong Yu,&nbsp;Lingfeng Kong,&nbsp;Shikai Liu,&nbsp;Shaojun Du,&nbsp;Qi Li","doi":"10.1007/s10126-025-10425-0","DOIUrl":"10.1007/s10126-025-10425-0","url":null,"abstract":"<div><p>Mollusk shell is secreted through mantle folds and exhibits color polymorphism predominantly caused by pigments. Mantle tissue consists of three folds, with the outer fold playing crucial roles in shell formation. Among various pigments influencing shell color, porphyrins—especially protoporphyrin IX (PPIX)—significantly contribute to orange-shell phenotype. However, the distinct functions of mantle folds in shell coloration and pigmentation remain poorly understood. In this study, the frozen section of fresh mantles from orange and gold shell-color Pacific oyster <i>Crassostrea gigas</i> was observed to have distinct porphyrin distributions. Significant differences in porphyrin spectrum and precise PPIX concentrations were detected among mantle folds of shell-color strains <i>C. gigas</i>. In addition, key genes from porphyrin pathway: protoporphyrinogen oxidase (PPOX) and ferrochelatase (FECH) were characterized to exhibit distinct expression patterns among mantle folds of different shell-colored oysters. Dual-label fluorescence in situ hybridization analysis of <i>Cg</i>PPOX and <i>Cg</i>FECH with the PPIX translocator protein (<i>Cg</i>TspO) verified the predominant functional location of these genes is the oyster outer mantle fold and periostracal groove. Furthermore, in vivo knockdown of <i>Cg</i>PPOX and <i>Cg</i>FECH verified their roles in PPIX metabolism, resulting in significant changes in porphyrin metabolic-related gene expression and altered PPIX concentrations in both the outer mantle fold and the newly deposited shell of <i>C. gigas</i>. This study identified the differential mantle fold porphyrin distribution and demonstrated essential roles of <i>Cg</i>PPOX and <i>Cg</i>FECH in PPIX metabolism, correlating with variations in PPIX content and shell color of <i>C. gigas</i>. The results provide new insights into molecular mechanisms underlying shell-color polymorphism in mollusks.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Delta-7 Alkenone Desaturase in Haptophyte Gephyrocapsa huxleyi Through Heterologous Expression in Tisochrysis lutea","authors":"Kohei Yoneda,&nbsp;Chinatsu Kobayashi,&nbsp;Hiroya Araie,&nbsp;Rikuri Morita,&nbsp;Ryuhei Harada,&nbsp;Yasuteru Shigeta,&nbsp;Hirotoshi Endo,&nbsp;Yoshiaki Maeda,&nbsp;Iwane Suzuki","doi":"10.1007/s10126-025-10427-y","DOIUrl":"10.1007/s10126-025-10427-y","url":null,"abstract":"<div><p>The marine haptophyte <i>Gephyrocapsa huxleyi</i> is an ecologically and geochemically important phytoplankton due to its contribution to the global carbon cycle and its ability to biosynthesize certain alkenones. These alkenones are long-chain alkyl ketones with two to four <i>trans</i>-type double bonds. The genes encoding alkenone desaturase in <i>G. huxleyi</i> have not been experimentally characterized so far, partly due to the difficulty of inducing genetic transformation in <i>G. huxleyi</i>. Therefore, we introduced the putative alkenone delta-7 desaturase of <i>G. huxleyi</i> (designated “DesT”) to the transformable and alkenone-producing haptophyte <i>Tisochrysis lutea</i>. We found two types of coding sequences for DesT, which are probably derived from the expression products of different alleles, and designated them “DesT-1” and “DesT-2.” The ratio of C_37:3 to C_37:2 methyl alkenone in the DesT-1 transformant was significantly higher than that in the mock strain that expressed only the hygromycin resistance gene, suggesting that DesT-1 was an alkenone delta-7 desaturase in <i>G. huxleyi</i>. In the protein structure, a tunnel where a substrate alkenone penetrates was predicted to be located around the histidine box of DesT, and hydrophilic and hydrophobic amino acids were respectively located at the proximal (near side to the histidine box) and distal ends of the tunnel. This is the first study to conduct experimental characterization of the alkenone metabolism-related gene in <i>G. huxleyi</i>. The heterologous expression system using <i>T. lutea</i> paves the way for further characterization of the alkenone metabolism-related genes in less transformable haptophytes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10427-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Signature of Lipid Production in Australian Aurantiochytrium sp. TC20","authors":"Kim Jye Lee Chang,&nbsp;Eduardo Gorron Gomez,&nbsp;Esmaeil Ebrahimie,&nbsp;Manijeh Mohammadi Dehcheshmeh,&nbsp;Dion M. F. Frampton,&nbsp;Xue-Rong Zhou","doi":"10.1007/s10126-025-10415-2","DOIUrl":"10.1007/s10126-025-10415-2","url":null,"abstract":"<div><p><i>Aurantiochytrium</i> not only excels in producing long-chain polyunsaturated fatty acids such as docosahexaenoic acid for humans, but it is also a source of essential fatty acids with minimal impacts on wild fisheries and is vital in the transfer of atmospheric carbon to oceanic carbon sinks and cycles. This study aims to unveil the systems biology of lipid production in the Australian <i>Aurantiochytrium</i> sp. TC20 by comparing the transcriptomic profiles under optimal growth conditions with increased fatty acid production from the early (Day 1) to late exponential growth phase (Day 3). Particular attention was paid to 227 manually annotated genes involved in lipid metabolism, such as <i>FAS</i> (fatty acid synthetase) and subunits of polyunsaturated fatty acids (<i>PUFA</i>) synthase. PCA analysis showed that differentially expressed genes, related to lipid metabolism, efficiently discriminated Day 3 samples from Day 1, highlighting the key robustness of the developed lipid-biosynthesis signature. Highly significant (pFDR &lt; 0.01) upregulation of polyunsaturated fatty acid synthase subunit B (<i>PFAB</i>) involved in fatty acid synthesis, lipid droplet protein (<i>TLDP</i>) involved in TAG-synthesis, and phosphoglycerate mutase (<i>PGAM-2</i>) involved in glycolysis and gluconeogenesis were observed. KEGG enrichment analysis highlighted significant enrichment of the biosynthesis of unsaturated fatty acids (pFDR &lt; 0.01) and carbon metabolism pathways (pFDR &lt; 0.01). This study provides a comprehensive overview of the transcriptional landscape of Australian <i>Aurantiochytrium</i> sp. TC20 in the process of fatty acid production.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10415-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143184662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in Preparation Technology and Functional Research On Marine Bioactive Peptides","authors":"Jing Wang,&nbsp;Fengcheng Li,&nbsp;Wenjun Li,&nbsp;Yueming Li,&nbsp;Jian Zhang,&nbsp;Song Qin","doi":"10.1007/s10126-024-10401-0","DOIUrl":"10.1007/s10126-024-10401-0","url":null,"abstract":"<div><p>Marine bioactive peptides are a class of peptides derived from marine organisms that can optimize the body’s metabolic environment and benefit the body’s health. These peptides have attracted increasing amounts of attention due to their wide range of health-promoting effects. Additionally, they have the potential to ameliorate diseases such as hypertension, diabetes, influenza viruses, and inflammation and can be used as functional foods or nutritional supplements for the purpose of treating or alleviating diseases. This paper reviews the recent research progress on marine bioactive peptides, focusing on their production technologies and functions in biomaterials and drug development.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143184643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Sequencing Reveals Effects of Artificial Feed Domestication on Intestinal Performance and Gene Expression of Carnivorous Mandarin Fish (Siniperca chuatsi) and Related Mechanisms","authors":"Jia-Xing Luo,&nbsp;Xiao-Tian Gao,&nbsp;Zhen Rong,&nbsp;Li-Han Zhang,&nbsp;Yan-Feng Sun,&nbsp;Zun-Li Qi,&nbsp;Qi Yu,&nbsp;Khor Waiho,&nbsp;Wei-Xu Zhao,&nbsp;Yi-Huan Xu,&nbsp;Chun-Long Zhao,&nbsp;Cheng-Bin Wu","doi":"10.1007/s10126-025-10420-5","DOIUrl":"10.1007/s10126-025-10420-5","url":null,"abstract":"<div><p>Mandarin fish (<i>Siniperca chuatsi</i>) is a voracious carnivorous species, usually consuming only live bait fish, but dietary acclimation enables it to accept artificial feed. However, the effects of dietary acclimation on intestinal performance and gene expression in mandarin fish and related mechanisms remain largely unknown. Therefore, this study investigated the effects of artificial feed on intestinal physicochemical and biochemical performance and gene expression in mandarin fish. Mandarin fish were sampled on day 10 after feeding with live dace (LD), at day 40 after subsequent feeding with dead dace plus artificial feed (DD + AF) from day 11 to day 40, and at day 90 after continuous feeding with artificial feed (AF) alone from day 41 to day 90 for transcriptome sequencing. The biochemical analysis results indicated that artificial feed significantly increased the activity of antioxidant enzymes glutathione peroxidase and superoxide dismutase in the intestine, liver, and stomach. Histological analysis demonstrated intestinal damage in mandarin fish fed with artificial feed. The GO and KEGG enrichment analyses indicated that the DEGs in AF vs. DD + AF were significantly enriched in the pentose phosphate pathway, and the DEGs in AF vs. LD were mainly significantly enriched in glycolysis/gluconeogenesis and PPAR signaling pathways. Nineteen feed acclimation–related key genes such as gene <i>pfkfb4a</i> and <i>scd</i> were identified in the intestine and found to exhibit upregulated expressions. These results revealed that artificial feed domestication enhanced the antioxidant capacity of the mandarin fish intestine and reduced hepatic lipid deposition by upregulating the related gene expression of mandarin fish and that the regulation of carbon metabolisms, including sugar, lipid, and steroid metabolisms, might be fundamental mechanisms for mandarin fish to acclimatize to dietary changes. These findings provide novel insights into the feed acclimation mechanism of mandarin fish, holding implications for promoting large-scale artificial feed aquaculture of mandarin fish and improving economic efficiency.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}