Marine Biotechnology最新文献

{"title":"Bacteria-Produced Algicide for Field Control of Toxic Dinoflagellates Does Not Cause a Cortisol Stress Response in Two Estuarine Fish Species","authors":"Victoria E. Simons,&nbsp;Timothy E. Targett,&nbsp;Patrick M. Gaffney,&nbsp;Kathryn J. Coyne","doi":"10.1007/s10126-024-10383-z","DOIUrl":"10.1007/s10126-024-10383-z","url":null,"abstract":"<div><p>Application of algicides produced by naturally occurring bacteria is considered an environmentally friendly approach to control harmful algal blooms. However, few studies assess the effects of bacterial algicides on non-target species, either independently or with other stressors. Here, we measured sub-lethal effects of dinoflagellate-specific algicide IRI-160AA on the estuarine fish <i>Fundulus heteroclitus</i> and <i>Menidia menidia</i> in laboratory experiments. Plasma cortisol levels were measured to test whether a neuroendocrine stress response was induced in these fish following exposure to the algicide alone, and in combination with diel-cycling hypoxia and/or pH, at 25 and 30 °C. Results show that exposure to IRI-160AA does not significantly affect cortisol levels in either species, at either temperature tested, whether exposure occurs independently or with co-occurring hypoxia and/or pH cycles as potential multiple stressors. These results support the application of IRI-160AA as an environmentally friendly approach to control harmful algal blooms in estuarine environments.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10383-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation of Somatic Tissues in Oysters is Influenced by Sex and Heredity","authors":"Wen Teng,&nbsp;Chengxun Xu,&nbsp;Shikai Liu,&nbsp;Hong Yu,&nbsp;Lingfeng Kong,&nbsp;Qi Li","doi":"10.1007/s10126-024-10409-6","DOIUrl":"10.1007/s10126-024-10409-6","url":null,"abstract":"<div><p>The influence of sex and heredity on DNA methylation in the somatic tissues of mice has been well-documented, with similar hereditary effects reported in honeybees. However, the extent to which these factors affect DNA methylation in molluscan somatic tissues remains poorly understood. In this study, we investigated genomic DNA methylation patterns in the adductor muscle of two genetically distinct oyster strains using whole-genome bisulfite sequencing (WGBS). Our analysis identified significant differences in DNA methylation between sexes, with females exhibiting a global reduction compared to males. Furthermore, approximately half of the differentially methylated sites between the two parental strains were conserved in their offspring. Regions with differential methylation in parents typically exhibited intermediate methylation levels in the F1 progeny, whereas consistently methylated regions in parents maintained similar methylation levels in their progeny. These findings suggest that offspring DNA methylation is strongly influenced by parental methylation profiles, highlighting its potential role in sexual determination in oysters.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Mechanism Analysis Related to Cold Tolerance of Red Swamp Crayfish, Procambarus clarkii","authors":"Jihu Zhang,&nbsp;Cheng-long Zhang,&nbsp;Hong Ju Chen,&nbsp;Xiang Shan Ji,&nbsp;Yan Zhao","doi":"10.1007/s10126-024-10408-7","DOIUrl":"10.1007/s10126-024-10408-7","url":null,"abstract":"<div><p>In China, the red swamp crayfish (<i>Procambarus clarkii</i>), a notorious invasive species, has become an important economic freshwater species. In order to compare the genetic diversity and population structure of crayfish from northern and southern China, we collected 60 crayfish individuals from 4 crayfish populations in northern China and 2 populations in southern China for sequencing using the 2b-RAD technique. Additionally, the whole genome sequence information obtained by 2b-RAD of 90 individuals from 2 populations in northern China and 7 populations in southern China were downloaded from NCBI. After quality control, a total of 25,371 SNPs were detected from approximately 54.22 billion raw reads. Based on these SNPs, high genetic diversity was observed in the 15 crayfish populations in China. The pairwise <i>F</i>_ST values indicated that there was a large genetic differentiation of crayfish populations in northern and southern China. Despite common genetic backgrounds, due to geographical barriers, genetic divergence has been observed in northern and southern China crayfishes. The principal component analysis in combination with Admixture and Neighbor-Joining tree analysis showed that the crayfish fell into two clusters corresponding to geographical regions. The integrated analysis of whole genome and transcriptome data showed that two genes (<i>CETN4</i> and <i>CPEB2</i>) might play important roles during crayfish resistance to a cold environment. This study reveals the genetic differentiation of crayfish populations in northern and southern China and provides clues to the genetic mechanism related to cold adaptation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Chromatography Test Strip of Exonuclease III-Amplified Aptamer for Rapid Identification of Prorocentrum minimum","authors":"Fuguo Liu,&nbsp;Chunyun Zhang,&nbsp;Jiahao Li,&nbsp;Jinju Ma,&nbsp;Yu Duan,&nbsp;Yuanyuan Wang,&nbsp;Guofu Chen","doi":"10.1007/s10126-024-10407-8","DOIUrl":"10.1007/s10126-024-10407-8","url":null,"abstract":"<div><p>Recently, the scale and frequency of harmful algae blooms (HABs) have gradually increased, posing a serious threat to human health, marine ecosystems and economic development. For early warning, a method is required that can quickly detect and monitor microalgae. It is proposed to use aptamer targeted to <i>Prorocentrum minimum</i>, along with exonuclease III (Exo III), gold nanoparticles, target single-stranded DNA and hairpin structure probe to construct a new method, i.e. aptamer-lateral flow dipstick (LFD) based on Exo III-assisted signal amplification assay (ALBEA). The key conditions, including signal amplification and LFD detection, are optimized. Under the optimal conditions, the detection limit of ALBEA was 1.25 cells mL⁻¹. The cross-reactivity test showed no positive result except for <i>P. minimum</i>, indicating that the method is highly specific. The anti-interference test confirmed that the technique was not affected by the presence of other microalgae. The tested results of <i>P. minimum</i> cultured under different nutrient conditions and different growth stages demonstrated that the method is not affected by the cell state. Furthermore, the test results of simulated natural water samples further validated the practicality of the ALBEA. In conclusion, the established ALBEA offers a sensitive, specific and user-friendly tool, which can be used for the rapid detection of <i>P. minimum</i> and also provides a reference for the detection of other microalgae.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Basis and Identification of Candidate Genes for Alkalinity Tolerance Trait in Spotted Sea Bass (Lateolabrax maculatus) by Genome-Wide Association Study (GWAS)","authors":"Yonghang Zhang,&nbsp;Chong Zhang,&nbsp;Haishen Wen,&nbsp;Xin Qi,&nbsp;Qing Wang,&nbsp;Kaiqiang Zhang,&nbsp;Lingyu Wang,&nbsp;Donglei Sun,&nbsp;Yani Dong,&nbsp;Pengyu Li,&nbsp;Yun Li","doi":"10.1007/s10126-024-10405-w","DOIUrl":"10.1007/s10126-024-10405-w","url":null,"abstract":"<div><p>Given the challenges of overcrowded coastal aquaculture spaces and insufficient production, utilizing saline-alkaline water areas represents a vital strategy to alleviate these bottlenecks. Spotted sea bass (<i>Lateolabrax maculatus</i>), with its formidable osmoregulatory capabilities, is an ideal candidate to develop a saline-alkaline tolerant strain. In our study, genotypic and phenotypic data from 287 <i>L. maculatus</i> individuals exposed to carbonate alkaline conditions were collected, and a genome-wide association study (GWAS) conducted to elucidate genetic basis related to carbonate alkalinity tolerance trait. Results showed that 14 SNPs and 8 InDels were markedly related to carbonate alkalinity tolerance trait, and 404 candidate genes were pinpointed within a ± 300-kb region surrounding these variants. Notably, the most significant SNP (SNP_05_17240108), along with two adjacent SNPs (SNP_05_17240102 and SNP_05_17240340) and two InDels (InDel_05_17240228 and InDel_05_17240231), was situated in the intron region of <i>trio</i> gene that could play vital roles in cell remodeling, and cell junction and activity of aquaporins to deal with carbonate alkalinity stress. Furthermore, candidate genes were significantly involved in pathways associated with carbohydrate metabolism, cell remodeling, ion transport, and RNA degradation, which were consistent with RNA-Seq analysis results of gills and kidneys in response to alkalinity stress. Our study will contribute to elucidate the genetic basis of alkalinity tolerance and the identified SNPs and InDels could be used for marker-assisted selection (MAS) and genomic selection (GS) for alkalinity tolerance trait in the breeding programs of spotted sea bass.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sargassum glaucescens Extract/Marine-Derived Collagen Blend Sponge and Their Properties for Wound Healing","authors":"Tayyeb Ghadimi,&nbsp;Noorahmad Latifi,&nbsp;Ahmad Hivechi,&nbsp;Vahid Hosseinpour Sarmadi,&nbsp;Sina Bayat Shahbazi,&nbsp;Naser Amini,&nbsp;Peiman B. Milan,&nbsp;Abolfazl Abbaszadeh,&nbsp;Ghazaleh Larijani,&nbsp;Hamid Fathalian,&nbsp;Shokoufeh Mortazavi,&nbsp;Fatemeh Latifi,&nbsp;Fatemeh Ghadimi,&nbsp;Siamak Farokh Forghani,&nbsp;Soheila Naderi Gharahgheshlagh","doi":"10.1007/s10126-024-10402-z","DOIUrl":"10.1007/s10126-024-10402-z","url":null,"abstract":"<div><p>Burn wounds are challenging to treat due to considerable tissue damage and fluid loss. Creating wound dressings from natural and biological materials makes it possible to treat wounds and promote rapid epithelialization to speed healing and restore skin function. As a result, the ability of a collagen scaffold (Col) made from rainbow trout (<i>Oncorhynchus mykiss</i>) and putative bioactive phytochemical components from a <i>Sargassum glaucescens (S. glaucescens)</i> extract (SGE) to promote burn wound healing was assessed in this work. Synthesized collagen (40 mg/ml)/SGE (1–3 mg/ml) samples were then characterized physiochemically and physiologically. The physicochemical examination validated the bioactive component of SGE, the type of collagen (type I, α1, and α2), the successful incorporation of SGE into collagen scaffolds (Col/SGE), the thermal stability, and excellent hydrophilicity and water absorption capacity of produced scaffolds. Moreover, biological experiments approved the excellent antioxidant and antibacterial activity of SGE, structural stability improvement against degradation, and cell proliferation enhancement without cell toxicity. The results showed the Col/SGE 3 mg/ml sample also had the highest level of cell activity, according to the antibacterial and cell viability assays. Additionally, using Col/SGE in vivo on burn wounds in rat models demonstrated a quicker rate of wound healing with stronger re-epithelialization and dermal remodeling, fewer inflammatory cells, more fibroblast cells, and great collagen buildup. Therefore, since the collagen/SGE scaffold is structurally stable and can potentially promote cell proliferation without causing cell toxicity, the acquired results suggested that it may significantly impact wound healing.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142919053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Single Nucleotide Polymorphisms in Cysteamine Dioxygenase Gene with Taurine Content in Oyster Crassostrea gigas","authors":"Shiqing Sun,&nbsp;Ning Kong,&nbsp;Xiang Li,&nbsp;Chunyu Jiang,&nbsp;Mingyu Xue,&nbsp;Lingling Wang,&nbsp;Linsheng Song","doi":"10.1007/s10126-024-10404-x","DOIUrl":"10.1007/s10126-024-10404-x","url":null,"abstract":"<div><p>The Pacific oyster <i>Crassostrea gigas</i> is rich in taurine, a conditionally essential amino acid functioning in anti-oxidation, anti-inflammation, anti-aging, osmoregulation, and neuromodulation. Breeding oyster varieties with enhanced taurine content is significant to meet people’s demand for high-quality oysters. In the present study, polymorphisms in the oyster cysteamine dioxygenase (<i>Cg</i>ADO) gene that encodes the central enzyme of the cysteamine pathway for taurine synthesis were investigated, and their association with taurine content was assessed in the Changhai (CH) and Qinhuangdao (QHD) populations. A total of 47 single nucleotide polymorphism (SNP) loci were identified in the exonic region of <i>Cg</i>ADO through Sanger sequencing, with a synonymous SNP (c.415T&gt;C) showing a significant association with taurine content. Oysters with the CT genotype at c.415T&gt;C exhibited higher taurine content than those with the TT genotype (<i>p</i> &lt; 0.05). Moreover, a significant difference in the <i>Cg</i>ADO mRNA expression was observed between oysters with different genotypes, with higher expression in the CT genotype compared to the TT genotype (<i>p</i> &lt; 0.001). These findings indicate the potential influence of <i>Cg</i>ADO polymorphisms on taurine content in <i>C. gigas</i> and provide candidate functional markers for the selective breeding of oyster varieties with improved taurine levels.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142919052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Characterization of Bisexually Fertile Triploid Dwarf Surf Clam Mulinia lateralis","authors":"Ang Zhao,&nbsp;Zujing Yang,&nbsp;Haoran Wang,&nbsp;Hao Wang,&nbsp;Shuai Zhong,&nbsp;Chenhui Li,&nbsp;Yuehuan Zhang,&nbsp;Jingjie Hu,&nbsp;Zhenmin Bao,&nbsp;Xiaoting Huang","doi":"10.1007/s10126-024-10406-9","DOIUrl":"10.1007/s10126-024-10406-9","url":null,"abstract":"<div><p>Triploids are widely used to rapidly achieve genetic improvements of organisms due to their fast growth and enhanced environmental adaptability. Artificially induced triploids are generally considered to be infertile owing to the obvious inhibition of gonadal development. Recently, some fertile individuals with reduced advantages have been found in triploid bivalves, which is a notable deviation from the original intention of artificially inducing triploids. This study utilized dwarf surf clams (<i>Mulinia lateralis</i>), a promising model organism of bivalves, to develop a model for exploring the potential mechanism of triploid reproduction. The results showed that the optimal induction condition for triploid <i>M. lateralis</i>, determined by orthogonal experiments, was 0.5 mg/L cytochalasin B (CB) to inhibit PB2 for 20 min, resulting in a triploidy rate of 95.57% and a hatching rate of 60.25%. By tracking the development of <i>M. lateralis</i>, we found that the induced triploids could develop normally to maturity and exhibited significant growth and survival advantages post-metamorphosis. Although the triploidy rate exhibited a slight decline overtime, it remained high, with a ratio of 90.63% at 120 dpf. Histological observation confirmed that the gonadal development pattern of triploid <i>M. laterali</i> was similar to that of diploids, but it also showed characteristics such as developmental retardation, few mature gametes, and gamete gigantism. The dynamic expression of genes related to gonadal development provided further molecular evidence for this phenomenon. Additionally, 82.6% of triploid <i>M. laterali</i> exhibited normal spawning behavior, produced fewer but larger viable gametes, and could generate offspring with full developmental potential. Flow cytometry analysis revealed that sperm of triploid <i>M. laterali</i> was aneuploid, with a DNA content of about 1.5 times that of diploid sperm, and the ploidy levels of mating offspring were 2N (DD, diploid female × diploid male), 2.5N (DT, diploid female × triploid male), 2.5N (TD, triploid female × diploid male), and 3N (TT, triploid female × triploid male), respectively. Overall, the artificially induced triploid <i>M. laterali</i> has been confirmed to be bisexually fertile, which will provide a unique model for exploring the underlying mechanisms of advantageous trait formation and fertility regulation in triploids, and offer a valuable platform for the study of ploidy control and polyploidization in bivalves. Please check and confirm that the authors and their respective affiliations have been correctly identified and amend if necessary. Yes, i have checked and it is OK. </p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Sex Identification in Spotted Knifejaw (Oplegnathus punctatus) Using tmem88 Gene Structural Variation Markers","authors":"Pingrui Xu,&nbsp;Yongshuang Xiao,&nbsp;Junde Dong,&nbsp;Zhizhong Xiao,&nbsp;Jun Li,&nbsp;Yanfeng Wang","doi":"10.1007/s10126-024-10403-y","DOIUrl":"10.1007/s10126-024-10403-y","url":null,"abstract":"<div><p>Spotted knifejaw (<i>Oplegnathus punctatus</i>) is an economically important marine cultured species exhibiting a unique complex sex chromosome system (X₁X₁X₂X₂ in females and X₁X₂Y in males), with males possessing one fewer chromosome (2n = 47) than females (2n = 48) and an abnormally large Y chromosome. Additionally, males demonstrate significant growth advantages over females. Rapid and accurate sex identification is essential for effective culture management, selective breeding, and population control. In this study, we identified a homologous region of the <i>tmem88</i> gene containing large DNA insertion markers on the X and Y chromosomes through whole-genome sequencing of <i>O. punctatus</i>. The X₁ chromosome harbors a 278 bp DNA fragment, whereas the Y chromosome contains a 1472 bp fragment, resulting in a 1194 bp size difference indicative of structural variation in the non-coding region of the <i>tmem88</i> gene. We developed a rapid detection method based on this variation, utilizing a pair of primers that amplify two distinct bands (278 bp and 1472 bp) in male (X₁X₂Y) individuals and a single 278 bp band in female (X₁X₁X₂X₂) individuals when analyzed by agarose gel electrophoresis. This method enables efficient and accurate sex differentiation in <i>O. punctatus</i>, significantly reducing the time required for identification and enhancing detection efficiency. This study provides a valuable tool for the rapid identification of sex in <i>O. punctatus</i>, facilitating improved breeding strategies and supporting the large-scale production of high-quality fry.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10403-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Differentially Expressed Genes and Alternative Splicing Analysis Revealed the Difference in Virulence to American Eels (Anguilla rostrata) Infected by Edwardsiella anguillarum and Aeromonas hydrophila","authors":"Peng Lin,&nbsp;Zihao Chen,&nbsp;Guanghua Sun,&nbsp;Songlin Guo","doi":"10.1007/s10126-024-10400-1","DOIUrl":"10.1007/s10126-024-10400-1","url":null,"abstract":"","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}