Marine Biotechnology最新文献

{"title":"Lipid Droplet Degradation Through Lipophagy in Aurantiochytrium limacinum mh0186","authors":"Sheng Wu,&nbsp;Yohei Ishibashi,&nbsp;Masahiro Hayashi,&nbsp;Nozomu Okino","doi":"10.1007/s10126-025-10495-0","DOIUrl":"10.1007/s10126-025-10495-0","url":null,"abstract":"<div><p>Autophagy is a cellular recycling process degrading and reusing cytoplasmic components via lysosomes or vacuoles, whereas lipophagy is a specialized form of autophagy that degrades lipid droplets (LDs). Thraustochytrids are heterotrophic marine protists known for high polyunsaturated fatty acid (PUFA) production and as valuable models for lipid metabolism research. In this study, LD degradation in <i>Aurantiochytrium limacinum</i> mh0186 was characterized under glucose-starvation conditions. Glucose starvation robustly activated autophagy, evidenced by GFP-tagged autophagy-related protein 8 (Atg8) translocation into endosome-like vesicles. These vesicles engulfed LDs in a microautophagy-like process, later fusing with acidic vacuole-like organelles (VLOs) to facilitate LD degradation. Impaired autophagy inhibited LD degradation in endosome-like vesicles but triggered compensatory lipolysis through elevation of intracellular lipase activity, resulting in a significant decrease in triacylglycerol (TG) levels. Our findings revealed a dual regulatory network in which autophagy orchestrated LD degradation via endosome-like vesicles, whereas autophagy inhibition triggered compensatory lipolysis activation to sustain lipid degradation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-200b Regulates Cellular Proliferation and Inflammatory Responses by Targeting Dual-Specificity Phosphatase 1 in Lower Vertebrates, Sciaenops ocellatus","authors":"Jingyao Yu,&nbsp;Qing Chu,&nbsp;Lin Zhang","doi":"10.1007/s10126-025-10498-x","DOIUrl":"10.1007/s10126-025-10498-x","url":null,"abstract":"<div><p>DUSP1 is a representative member of the dual-specificity protein phosphatase family known to dephosphorylate MAPK family members and is crucial in the biosynthesis of proinflammatory cytokines. In mammals, DUSP1 is also involved in various functions including proliferation, differentiation, and apoptosis. However, little is known about the function and mechanism of DUSP1 regulating antibacterial immune response in teleost. MicroRNAs (miRNAs) have emerged as essential regulators with profound effects on immune and inflammation responses, but in teleost, the miRNA-mediated regulatory networks at different levels of signaling pathways remain largely unknown. In this study, the regulatory mechanism of the miR-200b-DUSP1-mediated inflammatory responses in teleost was addressed. We found that the expression of DUSP1 could be significantly regulated by <i>Vibrio harveyi</i> and lipopolysaccharide in <i>Sciaenops ocellatus</i>. Overexpression of DUSP1 resulted in the suppression of proinflammatory cytokine expression and cell proliferation, indicating that DUSP1 acts as a negative regulator in inflammatory responses. Furthermore, we found that miR-200b is a post-transcriptional regulator of DUSP1 that is highly expressed upon bacterial infections. Bacteria-induced miR-200b promoted cell proliferation and inflammatory responses through targeting <i>S. ocellatus</i> DUSP1 and increasing NF-κB signaling pathway. These results suggest that miR-200b plays a key role in promoting antibacterial immune responses through directly targeting the immune regulatory molecule DUSP1, which will greatly enrich the networks of host–pathogen interactions in lower vertebrates.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D3 Improves Growth Performance, Digestive Enzyme Activity, Antioxidant Capacity, Lipid Metabolism, Histomorphology, Ca and P Homeostasis and Ammonia Stress Resistance in Pacific White Shrimp (Penaeus vannamei)","authors":"Yein Lee,&nbsp;Kyeong-Jun Lee","doi":"10.1007/s10126-025-10497-y","DOIUrl":"10.1007/s10126-025-10497-y","url":null,"abstract":"<div><p>This study evaluated the effects of dietary vitamin D₃ (VD₃) levels on growth, lipid metabolism, antioxidant capacity, digestive enzyme activity, histomorphology and ammonia stress resistance in Pacific white shrimp (<i>Penaeus vannamei</i>) post-larvae. Shrimp were fed eight diets containing 0.2, 30.6, 74.0, 164, 278, 553, 1,128 and 2,491 μg/kg of VD₃ for 34 days. Shrimp fed VD164 and VD278 diets exhibited significantly higher growth, feed efficiency and ammonia stress resistance compared to the control (<i>P</i> &lt; 0.05). Carapace calcium levels increased in shrimp fed VD₃ ≥ 30.6 μg/kg, and phosphorus in those fed ≥ 278 μg/kg (<i>P</i> &lt; 0.05). Trypsin and lipase activities were elevated in all VD₃ groups except VD2491 group (<i>P</i> &lt; 0.05). Superoxide dismutase, catalase and glutathione peroxidase activities were higher in VD278 and VD553 groups compared to the control (<i>P</i> &lt; 0.05). Histological analysis revealed increased B and F cell counts, and reduced lipid droplets in the hepatopancreas of all VD₃ groups (<i>P</i> &lt; 0.05). Midgut villi were significantly longer in all VD₃ groups, except VD30.6, and muscle thickness increased in all treatment groups (<i>P</i> &lt; 0.05). VD278 upregulated expression of vitamin D receptor and genes related to lipid metabolism, indicating enhanced lipid utilization. A quadratic-plateau regression model estimated the optimal dietary VD₃ level for weight gain at 186.03 μg/kg. In conclusion, dietary VD₃ supplementation enhances growth, feed efficiency and ammonia stress resistance in <i>P. vannamei</i> by enhancing digestive enzyme activity, antioxidant capacity, lipid metabolism and tissue morphology.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-level Assembly of the Genome of Mesocentrotus nudus Reveals a Sex-Linked Region on Chromosome 7","authors":"Quanchao Wang,&nbsp;Yanxia Wang,&nbsp;Zhiyuan Dong,&nbsp;Yue Wang,&nbsp;Xiaojing Li","doi":"10.1007/s10126-025-10499-w","DOIUrl":"10.1007/s10126-025-10499-w","url":null,"abstract":"<div><p>The sea urchin <i>Mesocentrotus nudus</i> holds significant economic importance, making a comprehensive understanding of its sex differentiation and development mechanisms crucial for facilitating genetic enhancement. In this study, a high-quality chromosome-level assembly of the <i>M. nudus</i> genome was accomplished, spanning 707 Mb and encompassing 21 chromosomes, which together represented 97.6% of the assembly. Analysis of the locations of sex-linked markers indicated that sex determination in <i>M. nudus</i> may be predominantly governed by a confined region on chromosome 7. Within this specific area, an initial screening identified 37 protein-coding genes, encompassing 64 transcripts. Subsequent comparative transcriptome analysis revealed notable differences in the expression patterns of 11 genes (comprising 12 transcripts) between the testes and ovaries of <i>M. nudus</i>. In conclusion, this genome assembly serves as an invaluable resource for future investigations into the population genetics, evolutionary history, and genetic mechanism analysis of <i>M. nudus</i>. Moreover, the identification of the genomic location of the sex-linked region in <i>M. nudus</i>, coupled with the identification of candidate sex-determining genes, lends further credence to the notion of rapid evolution in sex-determining mechanisms among sea urchins.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights in Adriatic Seaweed (Ulva spp.): Nutraceutical and Bioactive Potential and Green Extraction Techniques for Pigments, Antioxidants, and Phenolic Compounds","authors":"Nada Ćujić Nikolić,&nbsp;Dragana Drakulović,&nbsp;Slađana Rakita,&nbsp;Ivana Čabarkapa,&nbsp;Jelena Vulić,&nbsp;Zorana Mutavski,&nbsp;Katarina Šavikin","doi":"10.1007/s10126-025-10490-5","DOIUrl":"10.1007/s10126-025-10490-5","url":null,"abstract":"<div><p>Seaweeds, particularly those from the genus <i>Ulva</i>, have gained increasing attention due to their health benefits for humans and their promising bioactive properties, which are applicable as pharmaceuticals and nutraceuticals. However, the potential of <i>Ulva</i> species from the Adriatic Sea (Boka Kotorska Bay) remains largely unexploited. This study aimed to optimize the extraction protocol for key bioactive compounds to maximize their utilization. The chemical composition, physicochemical characteristics, antioxidant capacity, and antimicrobial properties of <i>Ulva</i> spp. were evaluated. Dried seaweed contained 108.51 mg GAE/100 g of polyphenolics, along with chlorophyll <i>a</i> (19.57 mg/100 g) and chlorophyll <i>b</i> (24.02 mg/100 g) as natural green pigments. The antioxidant activity, assessed by DPPH, RP, and ABTS assays, exhibited the IC50 values of 1.727, 1.167, and 0.932 mg/mL, respectively. However, the antimicrobial activity against pathogens such as <i>S. aureus</i>, <i>B. cereus</i>, <i>E. coli</i>, and <i>P. aeruginosa</i> demonstrated low potency. FTIR analysis revealed various significant peaks of characteristic alcoholic and phenolic functional groups, indicating the presence of major phytochemical compounds, while thermal analysis confirmed the good stability of the dried macroalga up to 142 °C. Among the tested extraction methods, maceration (50% ethanol, 1:20 solid-to-solvent ratio) proved to be the most effective. Dried <i>Ulva</i> powder demonstrated a balanced n-6/n-3 ratio and a comprehensive profile of essential and non-essential amino acids beneficial for human nutrition. All of these findings support the potential of <i>Ulva</i> as a valuable marine resource, promoting and contributing to the development of sustainable seaweed-based industries.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Demonstration of Bromoform-Producing Gametophyte Culture for a Red Alga, Asparagopsis taxiformis in Laboratory Conditions","authors":"Ryuya Matsuda,&nbsp;Kazuyoshi Kuwano","doi":"10.1007/s10126-025-10493-2","DOIUrl":"10.1007/s10126-025-10493-2","url":null,"abstract":"<div><p><i>Asparagopsis taxiformis</i> is a seaweed of interest for use as a livestock feed ingredient because of its high bromoform content, effectively reducing methane emissions from ruminants. A reliable protocol covering hatchery and nursery phases is essential to develop aquaculture techniques for gametophytes. The aim of this study was to establish a seedling production method for gametophytes under controlled laboratory conditions. We conducted the following: (1) induction of tetraspore release, (2) assessment of juvenile gametophyte growth, and (3) evaluation of gametophyte quality through bromoform quantification. Tetraspore release was successfully induced at 25 °C under an equinox photoperiod (12 h light: 12 h dark) or at 20 °C under short photoperiod (8 h light: 16 h dark), with initial spore release occurring after 12.3 ± 1.03 and 15.1 ± 1.26 days, respectively. Germinated tetraspores developed into 3–5 cm gametophytes over 2 months through static culture, followed by an aeration phase using natural seawater. Artificial seawater failed to promote gametophyte growth. Aeration improved the daily growth rate (6.86 ± 0.36%) compared to that of the static culture (4.72 ± 0.51%). Further cultivation promoted the development of main axes, lateral branches, and rhizomes. Bromoform was predominantly accumulated in the lateral branches (17.8 ± 12.3 mg·g⁻¹ dry weight (DW)) and rhizomes (12.2 ± 1.37 mg·g⁻¹ DW), compared to main axes (5.27 ± 1.95 mg·g⁻¹ DW). Unlike tetrasporophytes, which constantly released bromoforms into the medium, gametophytes maintained stable extracellular bromoform levels. These findings demonstrate a feasible approach for laboratory-scale production of <i>A. taxiformis</i> gametophytes with consistent bromoform accumulation, contributing to the advancement of its aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced Sox2 Gene Expression Leads to Impaired Gonadal Development in Female Triploid Pacific Oyster, Crassostrea gigas","authors":"Qiong Yang,&nbsp;Hong Yu,&nbsp;Qi Li","doi":"10.1007/s10126-025-10496-z","DOIUrl":"10.1007/s10126-025-10496-z","url":null,"abstract":"<div><p>The decreased fecundity and irregular oogenesis are commonly observed in triploid animals. However, in triploid Pacific oysters (<i>Crassostrea gigas</i>), females exhibit variability in reproductive capacity, with some producing abundant oocytes while others are largely infertile. Currently, the molecular mechanisms underlying these differences in triploid oysters remain poorly understood. This study investigates the role of the <i>Sox2</i> transcription factor in oyster oogenesis and reproductive development. Quantitative PCR (qPCR) and in situ hybridization analysis revealed that <i>Sox2</i> expression was markedly higher in the gonads of diploid females but significantly reduced in triploid females, suggesting a potential association between diminished <i>Sox2</i> levels and impaired oogenesis in triploids. To test this hypothesis, RNA interference (RNAi) was used to knock down <i>Sox2</i> expression in female diploids. Knockdown of <i>Sox2</i> resulted in impaired gonadal development, disrupted oogenesis, and the appearance of abnormal germ cells resembling the β gonia observed in triploids. However, <i>Sox2</i> knockdown did not induce apoptosis in the diploid gonadal tissue. Transcriptomic analysis revealed downregulation of genes involved in cell cycle regulation, DNA replication and repair in <i>Sox2</i> knockdown <i>C. gigas</i>. Immunofluorescence staining of DNA damage markers showed elevated γH2AX levels in the gonads of <i>Sox2</i> knockdown oysters, indicating that decreased <i>Sox2</i> expression might result in defective DNA repair in germ cells of <i>C. gigas</i>. This study provides new insights into the molecular mechanisms underlying the sterility of triploid animals.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Expression Analysis of the MYB Gene Family in Gracilariopsis lemaneiformis to Reveal Potential Members Involved in High-Temperature Stress","authors":"Xueqing Yang,&nbsp;Wencheng Gong,&nbsp;Zhenyu Jin,&nbsp;Shiji Li,&nbsp;Zhijun Xiong,&nbsp;Xue Sun,&nbsp;Nianjun Xu,&nbsp;Shixia Liu","doi":"10.1007/s10126-025-10482-5","DOIUrl":"10.1007/s10126-025-10482-5","url":null,"abstract":"<div><p><i>Graci</i><i>lario</i><i>psis lemaneiformis</i>, as an economically important red alga, often suffers high-temperature stress which poses a threat to algal yield and even survival. It is important to achieve new varieties with stable traits and heat tolerance; hence, research on the discovery and functional analysis of high-temperature responsive genes of <i>G. lemaneiformis</i> is significant. MYB (<i>v-myb</i> avian myeloblastosis viral oncogene homolog) gene family is one of the largest transcription factor superfamilies in eukaryotes and has been proved to regulate multiple environmental stresses. However, the identification and comprehensive analysis of the MYB gene family in <i>G. lemaneiformis</i> has rarely been studied. In this study, we identified 18 MYB genes in <i>G. lemaneiformis</i> at the genome-wide level, including 15 1R-MYB/MYB-related, 2 R2R3-MYB, and 1 R1R2R3-MYB members. Among them, <i>GlMYB3</i>, <i>GlMYB4</i>, <i>GlMYB8</i>, <i>GlMYB9</i>, and GlMYB15 were remarkably induced under high temperature and were also upregulated by salicylic acid, methyl jasmonate, or abscisic acid. GlMYB3, GlMYB4, GlMYB9, and <i>GlMYB15</i> were localized in the nucleus when transiently expressed in <i>Nicotiana benthamiana</i> plants. <i>GlMYB4</i>, as one of the most strongly induced high temperature-associated genes, showed transactivation activity, and the C-terminal was critical for the transactivation activity. By yeast two-hybrid screening, GlMYB4 may interact with three candidate proteins: calcineurin subunit B (CNB), O-linked N-acetylglucosamine transferase (OGT), and cleavage and polyadenylation specificity factor (CPSF) to modulate high temperature tolerance.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of SNPs and Candidate Genes Associated with Fecundity Trait Using BSA-seq and RNA-seq in Exopalaemon carinicauda","authors":"Jiajia Wang,&nbsp;Qianqian Ge,&nbsp;Qiong Wang,&nbsp;Xiuhong Zhang,&nbsp;Jian Li,&nbsp;Jitao Li","doi":"10.1007/s10126-025-10492-3","DOIUrl":"10.1007/s10126-025-10492-3","url":null,"abstract":"<div><p>Reproductive traits are of paramount importance as economic traits in shrimps, playing a vital role in enhancing commercial larval production within the aquaculture industry. Identifying genetic markers associated with reproductive traits is essential for implementing genomic and marker-assisted selection strategies to enhance reproductive performance. However, the existing knowledge of such genetic markers in <i>Exopalaemon carinicauda</i> remains relatively limited. In this study, we utilized next-generation sequencing coupled with bulked segregant analysis to identify molecular markers and candidate genes associated with reproductive traits in <i>E. carinicauda</i> using a F₂ population. A total of 31,048,793 SNPs and 3193 indels were identified between the two pools. Six candidate regions, spanning 72.24 Mb on chromosomes 6, 14, 15, 21, 23, and 35, were consistently identified through Euclidean distance and ΔSNP-index analyses, encompassing 609 genes. Kyoto Encyclopedia of Genes and Genomes analysis revealed significant associations with pathways such as ovarian steroidogenesis, oocyte meiosis, insulin signaling, GnRH signaling, and estrogen signaling. Among these, 22 genes showed strong links to ovarian development based on comparative transcriptomic analysis across 4 ovarian development stages. The integrated analysis of whole genome and transcriptome data showed that four genes (juvenile hormone esterase carboxylesterase, aurora kinase A, insulin-like peptide receptor, and Argonaute 3) might play important roles in regulating reproductive traits of <i>E. carinicauda</i>. Five SNP loci were validated employing a general linear model that are significantly associated with fecundity traits in the F₂ population. These findings not only offer valuable insights into the genetic underpinnings of reproductive traits but also provide a strong foundation for the development of genomic and marker-assisted selection strategies aimed at enhancing fecundity traits in <i>E. carinicauda</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping of Growth-Associated SNP Markers in Asian Seabass (Latest calcarifer) Using the Marey Map Approach","authors":"Permkullaya Yuttiwat,&nbsp;Sila Sukhavachana,&nbsp;Naruechon Pattarapanyawong,&nbsp;Chontida Phuthaworn,&nbsp;Supawadee Poompuang","doi":"10.1007/s10126-025-10489-y","DOIUrl":"10.1007/s10126-025-10489-y","url":null,"abstract":"<div><p>The aims of this study were to investigate marker-growth trait associations and construct a SNP-based linkage map for an Asian seabass (KU) population from Thailand. First, we performed GWAS using phenotypes (weight and length) and genotypes (25,647 SNPs) for 1100 390-day-old fish. We selected the top 70 SNPs with allele substitution effects, ranging from 6.84 to 81.91 g for weight and from 0.12 to 1.8 cm for length for further analyses. Cross-validation of 70 SNPs using GBLUP yielded breeding value prediction accuracies 0.65 to 0.70. Second, we utilized previously published genome-wide SNPs and mapping data from multiple populations and the KU data to predict the genetic linkage map for the KU population using the Marey map approach. The KU linkage map consisted of 24 chromosomes spanning 567 Mb, with average marker spacing of 0.07 cM. The number of markers ranged from 598 to 1334, with an average of 1099 markers per chromosome. The predicted local recombination rates varied from 4.76 to 43.40 cM/Mb with an average of 2.35 cM/Mb. Lastly, Kompetitive allele-specific PCR (KASP) markers were developed from 70 SNPs and validated in 200 fish. The KASP assays were successful for all 70 SNPs in 196 fish with SNP call rates of 98.2 to 100%. Fifty-eight SNPs were found to have segregated across the genome, except for chromosomes 23 and 24. The linkage map obtained in this study provides a framework for future QTL mapping of growth-related traits in the KU population.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}