Marine Biotechnology最新文献

{"title":"Transcriptome Sequencing Reveals Effects of Artificial Feed Domestication on Intestinal Performance and Gene Expression of Carnivorous Mandarin Fish (Siniperca chuatsi) and Related Mechanisms","authors":"Jia-Xing Luo,&nbsp;Xiao-Tian Gao,&nbsp;Zhen Rong,&nbsp;Li-Han Zhang,&nbsp;Yan-Feng Sun,&nbsp;Zun-Li Qi,&nbsp;Qi Yu,&nbsp;Khor Waiho,&nbsp;Wei-Xu Zhao,&nbsp;Yi-Huan Xu,&nbsp;Chun-Long Zhao,&nbsp;Cheng-Bin Wu","doi":"10.1007/s10126-025-10420-5","DOIUrl":"10.1007/s10126-025-10420-5","url":null,"abstract":"<div><p>Mandarin fish (<i>Siniperca chuatsi</i>) is a voracious carnivorous species, usually consuming only live bait fish, but dietary acclimation enables it to accept artificial feed. However, the effects of dietary acclimation on intestinal performance and gene expression in mandarin fish and related mechanisms remain largely unknown. Therefore, this study investigated the effects of artificial feed on intestinal physicochemical and biochemical performance and gene expression in mandarin fish. Mandarin fish were sampled on day 10 after feeding with live dace (LD), at day 40 after subsequent feeding with dead dace plus artificial feed (DD + AF) from day 11 to day 40, and at day 90 after continuous feeding with artificial feed (AF) alone from day 41 to day 90 for transcriptome sequencing. The biochemical analysis results indicated that artificial feed significantly increased the activity of antioxidant enzymes glutathione peroxidase and superoxide dismutase in the intestine, liver, and stomach. Histological analysis demonstrated intestinal damage in mandarin fish fed with artificial feed. The GO and KEGG enrichment analyses indicated that the DEGs in AF vs. DD + AF were significantly enriched in the pentose phosphate pathway, and the DEGs in AF vs. LD were mainly significantly enriched in glycolysis/gluconeogenesis and PPAR signaling pathways. Nineteen feed acclimation–related key genes such as gene <i>pfkfb4a</i> and <i>scd</i> were identified in the intestine and found to exhibit upregulated expressions. These results revealed that artificial feed domestication enhanced the antioxidant capacity of the mandarin fish intestine and reduced hepatic lipid deposition by upregulating the related gene expression of mandarin fish and that the regulation of carbon metabolisms, including sugar, lipid, and steroid metabolisms, might be fundamental mechanisms for mandarin fish to acclimatize to dietary changes. These findings provide novel insights into the feed acclimation mechanism of mandarin fish, holding implications for promoting large-scale artificial feed aquaculture of mandarin fish and improving economic efficiency.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Variation Analysis in the samd3/elf3 Intergenic Region of the Barred knifejaw (Oplegnathus fasciatus) and the Development of Molecular Marker for Efficient Sex Identification","authors":"Pingrui Xu,&nbsp;Yongshuang Xiao,&nbsp;Zhizhong Xiao,&nbsp;Jun Li","doi":"10.1007/s10126-025-10417-0","DOIUrl":"10.1007/s10126-025-10417-0","url":null,"abstract":"<div><p>The fish species <i>Oplegnathus fasciatus</i> exhibits an X₁X₁X₂X₂/X₁X₂Y sex determination mechanism. This species holds considerable economic value and displays pronounced sexual dimorphism in growth. Therefore, the development of a rapid and accurate method for sex identification is critical to enhancing breeding efficiency and maximizing production value. Using third-generation PacBio whole-genome sequencing, we identified a homologous region in the <i>samd3/elf3</i> intergenic region of the X and Y chromosomes of <i>O. fasciatus</i>. Analysis of the whole-genome sequence revealed a large DNA insertion marker fragment within this region. Using specifically designed primers, two bands of 390 bp and 1008 bp were successfully amplified in males, whereas only a single 390 bp band was detected in females. This marker can be easily distinguished by agarose gel electrophoresis, greatly enhancing the efficiency and accuracy of sex identification. This study not only expands the molecular marker system for sex identification of <i>O. fasciatus</i> but also offers a valuable methodological reference for sex identification in other economically important fish species. These findings have significant implications for germplasm improvement and efficient selection in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon (Oncorhynchus kisutch) Population Selected for High Growth Phenotype","authors":"Junya Kobayashi,&nbsp;Ryo Honda,&nbsp;Sho Hosoya,&nbsp;Yuki Nochiri,&nbsp;Keisuke Matsuzaki,&nbsp;Koichi Sugimoto,&nbsp;Atsushi J. Nagano,&nbsp;Akira Kumagai,&nbsp;Kiyoshi Kikuchi,&nbsp;Tadahide Kurokawa","doi":"10.1007/s10126-025-10416-1","DOIUrl":"10.1007/s10126-025-10416-1","url":null,"abstract":"<div><p>Selective breeding is a powerful tool for improving aquaculture production. A well-managed breeding program is essential, as populations can otherwise lose genetic diversity, leading to reduced selection response and inbreeding excesses. In such cases, genetic diversity in broodstock must be restored by introducing individuals from external populations. However, this can reduce the accumulated genetic gains from selective breeding. However, the selective introduction of individuals with superior phenotypes will allow the restoration of genetic diversity without sacrificing these gains. In this study, we demonstrated this possibility using a selectively bred (SB) and a randomly bred (RB) population of coho salmon (<i>Oncorhynchus kisutch</i>). Forty males with superior growth were selected from the RB population using genomic selection and crossed with 127 randomly collected females from the SB population, producing a newly bred (NB) population. Genetic diversity, assessed from population statistics such as effective number of alleles, allele richness, and observed heterozygosity of 11 microsatellite markers, was higher in NB than in SB and RB. Additionally, fork length and body weight were compared among the three populations after 12 months of growth post-fertilization in common tanks. The least-squares means of fork length and body weight were similar between NB (164.9 mm and 57.9 g) and SB (161.1 mm and 53.7 g), while both were significantly greater than RB (150.4 mm and 43.0 g). Our results highlight the effectiveness of genome-assisted gene flow in restoring the genetic diversity of a population without compromising accumulated genetic gain in growth.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Novel QTL on LG16 Associated with Acute Salt Tolerance in Red Tilapia (Oreochromis spp.) Using GWAS","authors":"Tong De Liu,&nbsp;Dan Dan Huang,&nbsp;Le Yi Chang,&nbsp;Tao Fei Qiao,&nbsp;Jun Hong Xia","doi":"10.1007/s10126-025-10422-3","DOIUrl":"10.1007/s10126-025-10422-3","url":null,"abstract":"<div><p>Culturing saline tilapia has become a new trend in the aquaculture due to the scarcity of freshwater resources. In this study, the genetic basis controlling for salt tolerance were investigated by using a ddRAD-seq-based GWAS in 288 individuals with extreme salt tolerant traits from half-sib families of red tilapia. 12 genome-wide significant SNPs and 6 chromosome-wide significant SNPs associated with acute salt tolerance were identified. Two QTLs on LG18:25,593,701–7009020 and on LG16:19,735,164–21,231,391 were defined. It is noteworthy that the QTL on LG16 is a novel QTL associated with acute salt stress. Near the significant SNP sites, we identified candidate genes <i>sik1</i>, <i>ltb4r2b, pnp5b</i> and <i>kirrel1b</i> with differential transcript expression under salt stress. Furthermore, significant physiological differences in serum osmolality and ion concentrations were confirmed between the tolerant group and sensitive group under 4.5 h of 22 ppt stress. The sensitive group had much higher serum osmolality (osmolality: 642.20 ± 6.30 mOsm/kg) and higher concentrations of sodium and chloride ions (sodium: 317.67 ± 5.03 mmol/L and chloride: 316.43 ± 8.28 mmol/L) than the tolerant group (547.60 ± 15.44 mOsm/kg, <i>p</i> _osmolality = 0.0002; sodium: 280.53 ± 9.13 mmol/L, <i>p</i> _sodium &lt; 0.0242; chloride: 266.00 ± 12.00 mmol/L, <i>p</i> _chloride &lt; 0.0184). However, the lowest bicarbonate concentration was detected in the sensitive group at 22 ppt (2.53 ± 0.30 mmol/L), which was significantly different from both the sensitive group at 0 ppt (<i>p</i> = 0.0008) and the tolerant group at 22 ppt (<i>p</i> = 0.0164). Our research laid the foundation for exploring the genetic mechanisms of acute salt tolerance and osmoregulation in red tilapia and for developing strains of red tilapia adapted to saltwater.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoperiod and Light Spectrum Modulate Daily Rhythms and Expression of Genes Involved in Cell Proliferation, DNA Repair, Apoptosis and Oxidative Stress in a Seabream Embryonic Stem Cell Line","authors":"Alba Vergès-Castillo,&nbsp;Patricia Herrera-Pérez,&nbsp;Carlos Pendón,&nbsp;Águeda J. Martín-Robles,&nbsp;José A. Muñoz-Cueto","doi":"10.1007/s10126-025-10418-z","DOIUrl":"10.1007/s10126-025-10418-z","url":null,"abstract":"<div><p>The use of cell lines as alternative models for environmental physiology studies opens a new window of possibilities and is becoming an increasingly used tool in marine research to fulfil the 3R’s rule. In this study, an embryonic monoclonal stem cell line obtained from a marine teleost (gilthead seabream, <i>Sparus aurata</i>) was employed to assess the effects of photoperiod (light/dark cycles <i>vs</i> constant dark) and light spectrum (white, blue, green, blue/green and red lights) on gene expression and rhythms of cellular markers of proliferation, DNA repair, apoptosis and cellular/oxidative stress by RT-qPCR and cosinor analyses. The results obtained revealed the optimal performance of cells under blue light (LDB), with all the genes analysed showing their highest RNA expression levels and most robust daily variations/rhythms in this condition. Under LDB, the mRNA levels of cell proliferation (<i>pcna</i>), DNA repair (<i>cry5</i>), anti-apoptotic (<i>bcl2</i>) and oxidative stress (<i>prdx2</i>) markers peaked at the day-night transition, whereas pro-apoptotic (<i>bax</i>) and cell stress (<i>hsp70</i>) markers showed their highest expression at the night-day transition, evidencing the strong synchronisation of the transcription of key genes involved in the cell cycle in this photoregime. The persistence of significant <i>pcna</i>, <i>cry5</i>, <i>hsp70</i> and <i>prdx2</i> rhythms after 3 days in constant darkness reveals the endogenous and circadian nature of these rhythms. Our results highlight the importance of implementing photoperiods with light–dark cycles of blue wavelengths when performing fish cell culture research. These results reinforce and extend our previous studies, confirming the importance of lighting conditions that mimic the natural environment for the proper development of fish embryos and larvae in aquaculture.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-Level Genome Assembly and Comparative Genomic Analysis of Planiliza haematocheilus: Insights into Environmental Adaptation and Hypoxia Tolerance Mechanisms","authors":"Ruizhi Wang,&nbsp;Na Song,&nbsp;Linlin Zhao","doi":"10.1007/s10126-025-10419-y","DOIUrl":"10.1007/s10126-025-10419-y","url":null,"abstract":"<div><p><i>Planiliza haematocheilus</i>, a teleostan species noted for its ecological adaptability and economic significance, thrives in both freshwater and marine environments. This study presents a novel chromosome-level genome assembly through Hi-C, PacBio CCS, and Illumina sequencing methods. The assembled genome has a final size of 651.58 Mb, with 24 chromosomes anchoring 91.94% of contigs. Contig N50 and scaffold N50 are respectively measured at 25.52 Mb and 28.59 Mb. Of the 22,476 protein-coding genes identified in the genome, 21,834 have functional annotations. BUSCO (Benchmarking Universal Single-Copy Orthologs) genome and gene annotation assessments yielded scores of 96% and 96.6%, respectively. The genome of <i>P. haematocheilus</i> revealed 228 expanded and 1433 contracted gene families. Comparative genomic analyses highlight adaptations and hypoxia tolerance, linked to protein synthesis, immune response, and metabolic regulation. The high-quality genome assembly supports advanced studies on gene expression patterns under different environmental stressors, contributing to genetic enhancement efforts for this economically important aquaculture species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Protective Efficacy in Rainbow Trout (Oncorhynchus mykiss) Against Vibrio anguillarum Through Immunization with a Combination of Formalin-Killed and Auxotrophic-Live V. anguillarum","authors":"Kyung Min Lee,&nbsp;Ki Hong Kim","doi":"10.1007/s10126-025-10414-3","DOIUrl":"10.1007/s10126-025-10414-3","url":null,"abstract":"<div><p>Vibriosis caused by <i>Vibrio anguillarum</i> has been an important bacterial disease in cultured rainbow trout (<i>Oncorhynchus mykiss</i>). In the present study, we evaluated the protective efficacy of a vaccine that consists of formalin-killed (FK) <i>V. anguillarum</i> and the <i>alr</i> genes knockout auxotrophic-live (AL) <i>V. anguillarum</i> (Δ<i>alr1</i>Δ<i>alr2 V. anguillarum</i>). Fish were immunized with a high dose of the FK <i>V. anguillarum</i> vaccine or four different combinations of FK and AL <i>V. anguillarum</i>. In the challenge test, fish immunized with 1 × 10⁶ CFU of FK <i>V. anguillarum</i> plus 1 × 10⁴ CFU of AL <i>V. anguillarum</i> (FK-10⁶ + AL-10⁴) showed complete protection (100% RPS) against <i>V. anguillarum</i>. In comparison, fish immunized with 1 × 10⁷ CFU of FK <i>V. anguillarum</i> (FK-10⁷) showed much lower survival rates. In the result of ELISA, the antibody titer of fish immunized with FK-10⁶ + AL-10⁴ was significantly higher than that of the PBS group, but the titer was not higher than FK-10⁷, suggesting that the higher protection by the FK + AL combination vaccine might be mediated by not only humoral immunity but also other protective factors conferred by live bacteria in the combination vaccine. In conclusion, the present FK + AL combination vaccine efficiently protected rainbow trout with approximately 10 times lower doses of bacteria than FK-10⁷, which could lessen the safety problem caused by a high-tittered live bacteria vaccine and the lower immunogenicity problem of killed bacteria vaccine.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals the Key Genes and Pathways for Growth, Ion Transport, and Oxidative Stress in Post-Larval Black Tiger Shrimp (Penaeus monodon) Under Acute Low Salt Stress","authors":"Yiming Li,&nbsp;Pengcheng Gao,&nbsp;Yucong Ye,&nbsp;Yan Li,&nbsp;Zhen Sun,&nbsp;Longyi Li,&nbsp;Kai Zhou,&nbsp;Yuxing Wei,&nbsp;Zongli Yao,&nbsp;Qifang Lai","doi":"10.1007/s10126-025-10411-6","DOIUrl":"10.1007/s10126-025-10411-6","url":null,"abstract":"<div><p>As an abiotic stress factor, salinity significantly affects the physiological activities of crustaceans. In this study, transcriptome sequencing was used to evaluate the mechanism of ion transport and the physiological response of black tiger shrimp (<i>Penaeus monodon</i>) under low salt stress. Four hundred post larval (PL) stage <i>P. monodon</i> were distributed in eight experimental tanks and exposed to 3 or 5 ppt salt concentrations for 96 h. Low salinity significantly reduced the survival rate of shrimp but simultaneously activated the activity of ion transporter enzymes Na⁺/K⁺-ATPase (NKA) and Ca²⁺/Mg²⁺-ATPase), the expression of <i>NKA</i>, galectin 10, and cytochrompe c peroxidase genes, and the activity and expression of antioxidant-related genes (superoxide dismutase, catalase, heat shock protein 60). Low salt stress activated the urea cycle but significantly inhibited glutathione metabolization-related indicators (glutamate dehydrogenase, glutaminase, glutamic acid). RNA-seq analysis identified 221 differentially expressed genes (78 up-regulated and 143 down-regulated). Quantitative real-time PCR and RNA-seq results of 11 of them were consistent, illustrating the validity of the transcriptomic predictions. Gene set enrichment analysis results showed that calcium ion transmembrane transport, calmodulin binding, the stress-activated protein kinase signaling cascade, and regulation of the cytosolic calcium ion concentration process were significantly enriched. These results showed that low salt stress activated the calcium-dominated ion transport pathway and promoted molting growth of <i>P. monodon</i>. They also indicate that there is potential for larval rearing shrimp under low salt conditions.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative Splicing Events and Differently Expressed Genes During Peak Mortality in Large Yellow Croaker (Larimichthys crocea) Infected with Scuticociliate","authors":"Jian Jin,&nbsp;Yang Li,&nbsp;Xiande Liu,&nbsp;Chunmei Yan,&nbsp;Zhiqiang Cheng,&nbsp;Yannian Wu,&nbsp;Zhiyong Wang,&nbsp;Dongling Zhang","doi":"10.1007/s10126-025-10413-4","DOIUrl":"10.1007/s10126-025-10413-4","url":null,"abstract":"<div><p>Large yellow croaker (<i>Larimichthys crocea</i>) is facing various threats from bacterial, viral, and parasitic diseases, especially scuticociliate. Scuticociliate is a facultative parasite causing high mortality in various marine fishes. In this study, an artificial scuticociliate infection model was successfully established for large yellow croaker. Comparative transcriptome analysis was performed on gill tissues collected from control fish and fish at the peak of mortality following exposure to the parasite to investigate the underlying molecular mechanisms of host-parasite interactions. A total of 400, 427, and 311 differential alternative splicing (DAS) events were identified at 7 d/0 h, 8 d/0 h, and 9 d/0 h, respectively. Meanwhile, 761 differentially expressed genes (DEGs) were found, with 154 simultaneously at three time points. GO and KEGG enrichment analysis showed that DAS genes and DEGs were mainly focused on self-respire, immune, and metabolic-related pathways. The DEGs related to blood coagulation included <i>fga</i>, <i>fgb</i>, <i>fgg</i>, and lectin domain genes. Lectin domain genes were also involved in reducing parasite burden. Cytokines, Caspase-1, <i>trim13</i>, <i>trim16</i>, and <i>trim39</i> co-participated in immune response. Notably, the complement component gene <i>c3</i> was both a DEG and underwent DAS. Using STRING software, interaction regulatory networks were constructed to visualize potential hub genes, revealing 22 DEGs shared across at least two time points. These findings provide valuable insights into the immune and metabolic responses of large yellow croaker to scuticociliate infection, offering a foundational reference for identifying resistant genes and understanding fish-parasite interactions.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory Effects of the Fucoidan from Sea Cucumber Apostichopus japonicus","authors":"Tingting Hou,&nbsp;Xiaolin Liu,&nbsp;Shuai Zhang,&nbsp;Kui Tang,&nbsp;Shilin Liu,&nbsp;Jialiang Liu,&nbsp;Xiaoteng Fan,&nbsp;Zaizhao Wang","doi":"10.1007/s10126-025-10410-7","DOIUrl":"10.1007/s10126-025-10410-7","url":null,"abstract":"<div><p>Fucoidan from <i>Apostichopus japonicus</i> (Aj-FUC) has shown anti-inflammatory activity, whereas its mechanism was not explicated. This study investigated the anti-inflammatory potential and mechanism of the fucoidan from green and purple <i>A. japonicus</i> (G-FUC and P-FUC) in lipopolysaccharide (LPS)-treated RAW264.7 cells. Results showed that Aj<i>-</i>FUCs at 25–400 µg/mL had no toxicity to cells after 24 h stimulation and promoted cell phagocytic activity. ELISA results indicated that Aj<i>-</i>FUC reduced the nitric oxide (NO), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 levels and increased IL-10 level. The Aj-FUC suppressed transcription of inflammatory-related genes (<i>tnf-α, il-1β</i>, <i>il-6</i>, <i>nlrp3</i>, <i>inos</i>, <i>cox-2</i>, <i>tlr4</i>, <i>trif</i>, and <i>nf-κb</i>) in LPS-treated RAW264.7 cells, among which G-FUC had stronger anti-inflammatory effects. Moreover, Aj<i>-</i>FUC upregulated the mRNA expression of autophagic genes (<i>beclin1</i>, <i>lc3II</i>, and <i>lamp2</i>). The immunoblotting and immunofluorescence analyses of Beclin-1 and LC3II supported that Aj<i>-</i>FUC enhanced autophagy activity. After autophagy inhibited by 3-methyladenine, the mRNA expressions of <i>tnf-α</i>, <i>il-6</i>, <i>il-1β</i>, and <i>nlrp3</i> were significantly upregulated in LPS-induced cells treated with Aj-FUC, suggesting the suppressed inflammation by Aj-FUC mediated via autophagy. Summarily, the present study demonstrated that Aj<i>-</i>FUC showed anti-inflammatory effects by elevating autophagy activity in LPS-induced macrophages.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}