Marine Biotechnology最新文献

{"title":"Activin Signaling Pathway Specialization During Embryonic and Skeletal Muscle Development in Rainbow Trout (Oncorhynchus mykiss)","authors":"Jasmine Richman,&nbsp;Michael Phelps","doi":"10.1007/s10126-024-10345-5","DOIUrl":"10.1007/s10126-024-10345-5","url":null,"abstract":"<div><p>Activin signaling is essential for proper embryonic, skeletal muscle, and reproductive development. Duplication of the pathway in teleost fish has enabled diversification of gene function across the pathway but how gene duplication influences the function of activin signaling in non-mammalian species is poorly understood. Full characterization of activin receptor signaling pathway expression was performed across embryonic development and during early skeletal muscle growth in rainbow trout (RBT, <i>Oncorhynchus mykiss</i>). Rainbow trout are a model salmonid species that have undergone two additional rounds of whole genome duplication. A small number of genes were expressed early in development and most genes increased expression throughout development. There was limited expression of activin Ab in RBT embryos despite these genes exhibiting significantly elevated expression in post-hatch skeletal muscle. CRISPR editing of the activin Aa1 ohnolog and subsequent production of meiotic gynogenetic offspring revealed that biallelic disruption of activin Aa1 did not result in developmental defects, as occurs with knockout of activin A in mammals. The majority of gynogenetic offspring exhibited homozygous activin Aa1 genotypes (wild type, in-frame, or frameshift) derived from the mosaic founder female. The research identifies mechanisms of specialization among the duplicated activin ohnologs across embryonic development and during periods of high muscle growth in larval and juvenile fish. The knowledge gained provides insights into potential viable gene-targeting approaches for engineering the activin receptor signaling pathway and establishes the feasibility of employing meiotic gynogenesis as a tool for producing homozygous F1 genome-edited fish for species with long-generation times, such as salmonids.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Heat-Responsive miRNAs and their Target Gene Regulation in Ruditapes philippinarum Under Acute Heat Stress","authors":"Changsheng Gao,&nbsp;Hongtao Nie","doi":"10.1007/s10126-024-10348-2","DOIUrl":"10.1007/s10126-024-10348-2","url":null,"abstract":"<div><p>This study aimed to investigate the inherent molecular regulatory mechanisms of <i>Ruditapes philippinarum</i> in response to extremely high-temperature environments and to enhance the sustainable development of the <i>R. philippinarum</i> aquaculture industry. In this study, we established a differential expression profile of miRNA under acute heat stress and identified a total of 46 known miRNAs and 80 novel miRNAs, three of which were detected to be significantly differentially expressed. We analyzed the functions of target genes regulated by differentially expressed miRNAs (DEMs) of <i>R. philippinarum</i>. The findings of the KEGG enrichment analysis revealed that 29 enriched pathways in the group were subjected to acute heat stress. Notably, fatty acid metabolism, <i>FoxO</i> signaling pathway, <i>TGF-β</i> signaling pathway, and ubiquitin-mediated proteolysis were found to play significant roles in response to acute heat stress. We established a regulatory map of DEMs and their target genes in response to heat stress and constructed the miRNA-mRNA regulation network. This study provides valuable insights into the response of <i>R. philippinarum</i> to high temperature, helping to understand its underlying molecular regulatory mechanisms under high-temperature stress.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141750809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics Analyses Explore the Immunometabolic Interplay in the Liver of White Crucian Carp (Carassius cuvieri) After Aeromonas veronii Challenge","authors":"Fei Wang,&nbsp;Zi-Rou Zhong,&nbsp;Qing Xie,&nbsp;Jie Ou,&nbsp;Ning-Xia Xiong,&nbsp;Ming-Zhu Huang,&nbsp;Shi-Yun Li,&nbsp;Gang Hu,&nbsp;Zi-Le Qin,&nbsp;Sheng-Wei Luo","doi":"10.1007/s10126-024-10347-3","DOIUrl":"10.1007/s10126-024-10347-3","url":null,"abstract":"<div><p><i>Aeromonas veronii</i> is one of the predominant pathogenic species that can imperil the survival of farmed fish. However, the interactive networks of immune regulation and metabolic response in <i>A</i>. <i>veronii</i>–infected fish are still unclear. In this investigation, we aimed to explore immunometabolic interplay in white crucian carp (WCC) after the <i>A</i>. <i>veronii</i> challenge. Elevated levels of immune-related genes were observed in various tissues after <i>A</i>. <i>veronii</i> infection, along with the sharp alteration of disease-related enzymatic activities. Besides, decreased levels of antioxidant status were observed in the liver, but most metabolic gene expressions increased dramatically. Multiomics analyses revealed that metabolic products of amino acids, such as formiminoglutamic acid (FIGLU), L-glutamate (L-Glu), and 4-hydroxyhippuric acid, were considered the crucial liver biomarkers in<i> A</i>. <i>veronii</i>–infected WCC. In addition, <i>A</i>. <i>veronii</i> infection may dysregulate endoplasmic reticulum (ER) function to affect the metabolic process of lipids, carbohydrates, and amino acids in the liver of WCC. These results may have a comprehensive implication for understanding immunometabolic response in WCC upon <i>A</i>. <i>veronii</i> infection.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation Profiling in Genetically Selected Clarias magur (Hamilton, 1822) Provides Insights into the Epigenetic Regulation of Growth and Development","authors":"K. Shasti Risha,&nbsp;Kiran D. Rasal,&nbsp;Dhalongsaih Reang,&nbsp;Mir Asif Iquebal,&nbsp;Arvind Sonwane,&nbsp;Manoj Brahmane,&nbsp;Aparna Chaudhari,&nbsp;Naresh Nagpure","doi":"10.1007/s10126-024-10346-4","DOIUrl":"10.1007/s10126-024-10346-4","url":null,"abstract":"<div><p>DNA methylation is an epigenetic alteration that impacts gene expression without changing the DNA sequence affecting an organism’s phenotype. This study utilized a reduced representation bisulfite sequencing (RRBS) approach to investigate the patterns of DNA methylation in genetically selected <i>Clarias magur</i> stocks. RRBS generated 249.22 million reads, with an average of 490,120 methylation sites detected in various parts of genes, including exons, introns, and intergenic regions. A total of 896 differentially methylated regions (DMRs) were identified; 356 and 540 were detected as hyper-methylated and hypo-methylated regions, respectively. The DMRs and their association with overlapping genes were explored using whole genome data of magur, which revealed 205 genes in exonic, 210 in intronic, and 480 in intergenic regions. The analysis identified the maximum number of genes enriched in biological processes such as RNA biosynthetic process, response to growth factors, nervous system development, neurogenesis, and anatomical structure morphogenesis. Differentially methylated genes (DMGs) such as <i>myrip</i>, <i>mylk3</i>, <i>mafb</i>, <i>egr3</i>, <i>ndnf</i>, <i>meis2a</i>, <i>foxn3</i>, <i>bmp1a</i>, <i>plxna3</i>, <i>fgf6</i>, <i>sipa1l1</i>, <i>mcu</i>, <i>cnot8</i>, <i>trim55b</i>, and <i>myof</i> were associated with growth and development. The selected DMGs were analyzed using real-time PCR, which showed altered mRNA expression levels. This work offers insights into the epigenetic mechanisms governing growth performance regulation in magur stocks. This work provides a valuable resource of epigenetic data that could be integrated into breeding programs to select high-performing individuals.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating Coral Thermal Stress via Inoculation with Quorum Quenching Bacteria","authors":"Qingsong Yang,&nbsp;Bing Yang,&nbsp;Bin Yang,&nbsp;Wenqian Zhang,&nbsp;Xiaoyu Tang,&nbsp;Huiming Sun,&nbsp;Yanying Zhang,&nbsp;Jie Li,&nbsp;Juan Ling,&nbsp;Junde Dong","doi":"10.1007/s10126-024-10344-6","DOIUrl":"10.1007/s10126-024-10344-6","url":null,"abstract":"<div><p>In the background of global warming, coral bleaching induced by elevated seawater temperature is the primary cause of coral reef degradation. Coral microbiome engineering using the beneficial microorganisms for corals (BMCs) has become a hot spot in the field of coral reef conservation and restoration. Investigating the potential of alleviating thermal stress by quorum quenching (QQ) bacteria may provide more tools for coral microbial engineering remediation. In this study, QQ bacteria strain <i>Pseudoalteromonas piscicida</i> SCSIO 43740 was screened among 75 coral-derived bacterial strains, and its quorum sensing inhibitor (QSI) compound was isolated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). Then, the thermal stress alleviating potential of QQ bacteria on coral <i>Pocillopora damicornis</i> was tested by a 30-day controlled experiment with three different treatments: control group (Con: 29 °C), high temperature group (HT: 31 °C), and the group of high temperature with QQ bacteria inoculation (HTQQ: 31 °C + QQ bacteria). The results showed that QQ bacteria SCSIO 43740 inoculation can significantly mitigate the loss of symbiotic algae and impairment of photosynthesis efficiency of coral <i>P. damicornis</i> under thermal stress. Significant difference in superoxide dismutase (SOD) and catalase (CAT) enzyme activities between HT and HTQQ was not observed. In addition, QQ bacteria inoculation suppressed the coral microbial community beta-dispersion and improved the stability of microbial co-occurrence network under thermal stress. It was suggested that QQ bacteria inoculation can alleviate coral thermal stress via reshaping microbial interaction and maintain community stability of coral microbiome. This study provided new evidence for the probiotic function of QQ bacteria in corals, which shedding light on the development of new microbiological tools for coral reef conservation.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Gene Alternative Splicing Events on Resistance to Cryptocaryonosis of Large Yellow Croaker (Larimichthys crocea)","authors":"Xin Li,&nbsp;Qiaozhen Ke,&nbsp;Ang Qu,&nbsp;Jiaying Wang,&nbsp;Ji Zhao,&nbsp;Peng Xu,&nbsp;Tao Zhou","doi":"10.1007/s10126-024-10342-8","DOIUrl":"10.1007/s10126-024-10342-8","url":null,"abstract":"<div><p>Large yellow croaker (<i>L. crocea</i>) is a productive species in marine aquaculture with great economic value in China. However, the sustainable development of large yellow croaker is hampered by various diseases including cryptocaryonosis caused by <i>Cryptocaryon irritans</i>. The genetic regulation processes for cryptocaryonosis in large yellow croaker are still unclear. In this present study, we analyzed differential alternative splicing events between a <i>C. irritans</i> resistance strain (RS) and a commercial strain (CS). We identified 678 differential alternative splicing (DAS) events from 453 genes in RS and 719 DAS events from 500 genes in CS. A set of genes that are specifically alternatively spliced in RS was identified including <i>mfap5</i>, <i>emp1</i>, and <i>trim33</i>. Further pathway analysis revealed that the specifically alternative spliced genes in RS were involved in innate immune responses through the PRR pathway and the Toll and Imd pathway, suggesting their important roles in the genetic regulation processes for cryptocaryonosis in large yellow croaker. This study would be helpful for the studies of the pathogenesis of cryptocaryonosis and dissection of <i>C. irritans</i> resistance for <i>L. crocea.</i></p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Single-Nucleotide Polymorphisms in Differentially Expressed Genes Favoring Soybean Meal Tolerance in Higher-Growth Zebrafish (Danio rerio)","authors":"Pilar E. Ulloa,&nbsp;Felipe Jilberto,&nbsp;Natalia Lam,&nbsp;Gonzalo Rincón,&nbsp;Luis Valenzuela,&nbsp;Valentina Cordova-Alarcón,&nbsp;Adrián J. Hernández,&nbsp;Patricio Dantagnan,&nbsp;Maria Cristina Ravanal,&nbsp;Sebastian Elgueta,&nbsp;Cristian Araneda","doi":"10.1007/s10126-024-10343-7","DOIUrl":"10.1007/s10126-024-10343-7","url":null,"abstract":"<div><p>Genetic variability within the same fish species could confer soybean meal (SBM) tolerance in some individuals, thus favoring growth. This study investigates the single-nucleotide polymorphisms (SNPs) in differentially expressed genes (DEGs) favoring SBM tolerance in higher-growth zebrafish (<i>Danio rerio</i>). In a previous work, nineteen families of zebrafish were fed a fish meal diet (100FM control diet) or SBM-based diets supplemented with saponin (50SBM + 2SPN–experimental diet), from juvenile to adult stages. Individuals were selected from families with a genotype-by-environment interaction higher (170 ± 18 mg) or lower (76 ± 10 mg) weight gain on 50SBM + 2SPN in relation to 100FM. Intestinal transcriptomic analysis using RNA-seq revealed six hundred and sixty-five differentially expressed genes in higher-growth fish fed 50SBM + 2SPN diet. In this work, using these results, 47 SNPs in DEGs were selected. These SNPs were genotyped by Sequenom in 340 zebrafish that were fed with a 50SBM + 2SPN diet or with 100FM diet. Marker-trait analysis revealed 4 SNPs associated with growth in 3 immunity-related genes (<i>aif1l</i>, <i>arid3c</i>, and <i>cst14b.2</i>) in response to the 50SBM + 2SPN diet (<i>p</i>-value &lt; 0.05). Two SNPs belonging to <i>aif1l</i> y <i>arid3c</i> produce a positive (+19 mg) and negative (−26 mg) effect on fish growth, respectively. These SNPs can be used as markers to improve the early selection of tolerant fish to SBM diet or other plant-based diets. These genes can be used as biomarkers to identify SNPs in commercial fish, thus contributing to the aquaculture sustainability.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Investigation to Discriminate Sexes in Alive Nile Tilapia (Oreochromis niloticus) Using Cyp19a1a and Dmrt1 Gene Expression in Tail Fin Tissues","authors":"Samy Y. El-Zaeem,&nbsp;Amr El-Hanafy,&nbsp;Alaa A. El-Dahhar,&nbsp;Ayaat M. Elmaghraby,&nbsp;Amany M. Hendy","doi":"10.1007/s10126-024-10340-w","DOIUrl":"10.1007/s10126-024-10340-w","url":null,"abstract":"<div><p>The Nile Tilapia (<i>Oreochromis niloticus</i>), a gonochoristic teleost fish with a XX/XY sex-determination system, is an ideal model for investigating gonadal sex differentiation. During gonadal differentiation, the expression of <i>cyp19a1a</i> in XX gonads and <i>dmrt1</i> in XY gonads are required for undifferentiated tissues to develop into ovary or testis. In this study, quantitative real-time RT-PCR assessed the expression of <i>cyp19a1a</i> and <i>dmrt1</i> genes in gonads and tail fin tissues. Differences in gene expression mean among sexually differentiated fish were analyzed using two-way analysis of variance (ANOVA) and validation of mixed model using discriminant analysis (DA) for morphometric traits and the gene expression in gonads and tail fin tissues used to validate and utilize them in discriminating sexes in sex-differentiated Nile Tilapia fish. The results revealed that, <i>cyp19a1a</i> gene expression in female ovaries was more significant than <i>dmrt1</i> in male testis. In the other hand, the <i>dmrt1</i> gene expression in the tail fin was higher in males than females. Both, <i>cyp19a1a</i> and <i>dmrt1</i> genes, can discriminate fish sexes by 100% by using their expression in tail fin tissues. In conclusion, the <i>cyp19a1a</i> and <i>dmrt1</i> genes could be used as a genetic marker to discriminate between the Nile Tilapia sexes, whereas used as an indicator for ovarian or testis differentiation in sexually differentiated Nile Tilapia using tail fin tissues. It is worth mentioning that this is the first investigation for using <i>cyp19a1a</i> and <i>dmrt1</i> genes from Nile Tilapia tail fin tissues in sex determination.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10340-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Selection of Large Yellow Croaker (Larimichthys crocea) with a High Plant Protein Diet Enhances the Growth Performance of Offspring","authors":"Qiaozhen Ke,&nbsp;Jiaxing Liu,&nbsp;Ji Zhao,&nbsp;Jiaying Wang,&nbsp;Pengxin Jiang,&nbsp;Yacheng Deng,&nbsp;Xiaoying Zhou,&nbsp;Junjia Zeng,&nbsp;Tao Zhou,&nbsp;Peng Xu","doi":"10.1007/s10126-024-10341-9","DOIUrl":"10.1007/s10126-024-10341-9","url":null,"abstract":"<div><p>Fishmeal is over-represented in the diets of large yellow croaker (<i>Larimichthys crocea</i>), and this farming mode, which relies heavily on fishmeal, is highly susceptible to the price of fishmeal and is unsustainable. Therefore, more and more studies on the large yellow croaker tend to replace fishmeal with land-based animal or plant proteins, but few studies have considered it from the genomic selection. In this study, we evaluated the survival rate (SR), final body weight (FBW), body weight gain (BWG), weight gain rate (WGR), and specific growth rate (SGR) of the large yellow croaker GS7 strain, which was obtained through genomic selection for tolerance to plant proteins and analyzed the differences in plant protein utilization between the GS7 strain and unselected commercial large yellow croaker (control group). The results of separate feeding for 60 days showed that although there was no significant difference in SR between the control and GS7 strains (<i>P</i> &gt; 0.05), the BWG, WGR, and SGR of the control were significantly lower (<i>P</i> &lt; 0.05) than those of the GS7 group. Results of mixed feeding after PIT marking showed that compared to the control fish, the GS7 strain had significantly higher BWG, WGR, and SGR (<i>P</i> &lt; 0.0001). To make the experimental results more precise, we compared fishes with equivalent initial body weight (IBW) in the GS7 strain and the control group. The final fish body weight (FBW) of Ctrl-2 (IBW 300–399 g) and Ctrl-4 (IBW 500–599 g) was significantly lower than those of the corresponding GS7-2 and GS7-4 (<i>P</i> &lt; 0.05), while the FBW of Ctrl-1 (IBW 200–299 g) and Ctrl-3 (IBW 400–499 g) was much significantly lower than the corresponding GS7-1 and GS7-3 (<i>P</i> &lt; 0.01). The BWG, WGR, and SGR of Ctrl-1 and Ctrl-4 were more significantly lower than those of the corresponding GS7-1 and GS7-4 (<i>P</i> &lt; 0.01), while the BWG, WGR, and SGR of Ctrl-2 and Ctrl-3 were more significantly different from the corresponding GS7-2 and GS7-3 (<i>P</i> &lt; 0.0001). Our results seem to point toward the same conclusion that the GS7 strain is better adapted to high plant protein diets than the unselected commercial large yellow croaker. These results will provide a reference for the low-fishmeal culture industry of large yellow croakers and the selection and breeding of strains tolerant to a high percentage of plant proteins in other marine fishes.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action","authors":"Athira P. P.,&nbsp;Anju M. V.,&nbsp;Anooja V. V.,&nbsp;Archana K.,&nbsp;Neelima S.,&nbsp;Muhammed Musthafa S.,&nbsp;I. S. Bright Singh,&nbsp;Rosamma Philip","doi":"10.1007/s10126-024-10338-4","DOIUrl":"10.1007/s10126-024-10338-4","url":null,"abstract":"<div><p>Antimicrobial peptides (AMPs), including beta-defensin from fish, are a crucial class of peptide medicines. The focus of the current study is the molecular and functional attributes of <i>Cm</i>Def, a 63-amino acid beta-defensin AMP from Malabar trevally, <i>Carangoides malabaricus</i>. This peptide demonstrated typical characteristics of AMPs, including hydrophobicity, amphipathic nature, and +2.8 net charge. The <i>Cm</i>Def was recombinantly expressed and the recombinant peptide, r<i>Cm</i>Def displayed a strong antimicrobial activity against bacterial fish pathogens with an MIC of 8 µM for <i>V. proteolyticus</i> and 32 µM for <i>A. hydrophila</i>. The <i>E. tarda</i> and <i>V. harveyi</i> showed an inhibition of 94% and 54%, respectively, at 32 µM concentration. No activity was observed against <i>V. fluvialis</i> and <i>V. alginolyticus.</i> The r<i>Cm</i>Def has a multimode of action that exerts an antibacterial effect by membrane depolarization followed by membrane permeabilization and ROS production. r<i>Cm</i>Def also exhibited anti-cancer activities in silico without causing hemolysis. The peptide demonstrated stability under various conditions, including different pH levels, temperatures, salts, and metal ions (KCl and CaCl₂), and remained stable in the presence of proteases such as trypsin and proteinase K at concentrations up to 0.2 µg/100 µl. The strong antibacterial efficacy and non-cytotoxic nature suggest that r<i>Cm</i>Def is a single-edged sword that can contribute significantly to aquaculture disease management.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}