Sox2基因表达降低导致雌性三倍体太平洋牡蛎性腺发育受损。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qiong Yang, Hong Yu, Qi Li
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引用次数: 0

摘要

在三倍体动物中,生殖力下降和卵发生不规律是很常见的。然而,在三倍体太平洋牡蛎(长牡蛎)中,雌性在生殖能力上表现出多样性,一些产生大量的卵母细胞,而另一些则基本上不育。目前,三倍体牡蛎中这些差异的分子机制仍然知之甚少。本研究探讨了Sox2转录因子在牡蛎卵发生和生殖发育中的作用。定量PCR (qPCR)和原位杂交分析显示,Sox2在二倍体雌性的性腺中表达显著升高,而在三倍体雌性的性腺中表达显著降低,提示Sox2水平降低与三倍体卵子发生障碍之间可能存在关联。为了验证这一假设,使用RNA干扰(RNAi)敲低Sox2在雌性二倍体中的表达。Sox2的敲低导致性腺发育受损,卵子发生中断,出现类似三倍体中观察到的β生殖腺的异常生殖细胞。然而,Sox2基因敲低不诱导二倍体性腺组织凋亡。转录组学分析显示,在Sox2基因敲低的gigas中,参与细胞周期调控、DNA复制和修复的基因下调。DNA损伤标记免疫荧光染色显示,Sox2基因敲除的牡蛎生殖腺中γ - h2ax水平升高,表明Sox2基因表达降低可能导致巨牡蛎生殖细胞DNA修复缺陷。这项研究为三倍体动物不育的分子机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reduced Sox2 Gene Expression Leads to Impaired Gonadal Development in Female Triploid Pacific Oyster, Crassostrea gigas.

The decreased fecundity and irregular oogenesis are commonly observed in triploid animals. However, in triploid Pacific oysters (Crassostrea gigas), females exhibit variability in reproductive capacity, with some producing abundant oocytes while others are largely infertile. Currently, the molecular mechanisms underlying these differences in triploid oysters remain poorly understood. This study investigates the role of the Sox2 transcription factor in oyster oogenesis and reproductive development. Quantitative PCR (qPCR) and in situ hybridization analysis revealed that Sox2 expression was markedly higher in the gonads of diploid females but significantly reduced in triploid females, suggesting a potential association between diminished Sox2 levels and impaired oogenesis in triploids. To test this hypothesis, RNA interference (RNAi) was used to knock down Sox2 expression in female diploids. Knockdown of Sox2 resulted in impaired gonadal development, disrupted oogenesis, and the appearance of abnormal germ cells resembling the β gonia observed in triploids. However, Sox2 knockdown did not induce apoptosis in the diploid gonadal tissue. Transcriptomic analysis revealed downregulation of genes involved in cell cycle regulation, DNA replication and repair in Sox2 knockdown C. gigas. Immunofluorescence staining of DNA damage markers showed elevated γH2AX levels in the gonads of Sox2 knockdown oysters, indicating that decreased Sox2 expression might result in defective DNA repair in germ cells of C. gigas. This study provides new insights into the molecular mechanisms underlying the sterility of triploid animals.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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