{"title":"薄叶蒿MYB基因家族的全基因组鉴定和表达分析揭示高温胁迫的潜在成员","authors":"Xueqing Yang, Wencheng Gong, Zhenyu Jin, Shiji Li, Zhijun Xiong, Xue Sun, Nianjun Xu, Shixia Liu","doi":"10.1007/s10126-025-10482-5","DOIUrl":null,"url":null,"abstract":"<p><p>Gracilariopsis lemaneiformis, as an economically important red alga, often suffers high-temperature stress which poses a threat to algal yield and even survival. It is important to achieve new varieties with stable traits and heat tolerance; hence, research on the discovery and functional analysis of high-temperature responsive genes of G. lemaneiformis is significant. MYB (v-myb avian myeloblastosis viral oncogene homolog) gene family is one of the largest transcription factor superfamilies in eukaryotes and has been proved to regulate multiple environmental stresses. However, the identification and comprehensive analysis of the MYB gene family in G. lemaneiformis has rarely been studied. In this study, we identified 18 MYB genes in G. lemaneiformis at the genome-wide level, including 15 1R-MYB/MYB-related, 2 R2R3-MYB, and 1 R1R2R3-MYB members. Among them, GlMYB3, GlMYB4, GlMYB8, GlMYB9, and GlMYB15 were remarkably induced under high temperature and were also upregulated by salicylic acid, methyl jasmonate, or abscisic acid. GlMYB3, GlMYB4, GlMYB9, and GlMYB15 were localized in the nucleus when transiently expressed in Nicotiana benthamiana plants. GlMYB4, as one of the most strongly induced high temperature-associated genes, showed transactivation activity, and the C-terminal was critical for the transactivation activity. By yeast two-hybrid screening, GlMYB4 may interact with three candidate proteins: calcineurin subunit B (CNB), O-linked N-acetylglucosamine transferase (OGT), and cleavage and polyadenylation specificity factor (CPSF) to modulate high temperature tolerance.</p>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 4","pages":"113"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-Wide Identification and Expression Analysis of the MYB Gene Family in Gracilariopsis lemaneiformis to Reveal Potential Members Involved in High-Temperature Stress.\",\"authors\":\"Xueqing Yang, Wencheng Gong, Zhenyu Jin, Shiji Li, Zhijun Xiong, Xue Sun, Nianjun Xu, Shixia Liu\",\"doi\":\"10.1007/s10126-025-10482-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gracilariopsis lemaneiformis, as an economically important red alga, often suffers high-temperature stress which poses a threat to algal yield and even survival. It is important to achieve new varieties with stable traits and heat tolerance; hence, research on the discovery and functional analysis of high-temperature responsive genes of G. lemaneiformis is significant. MYB (v-myb avian myeloblastosis viral oncogene homolog) gene family is one of the largest transcription factor superfamilies in eukaryotes and has been proved to regulate multiple environmental stresses. However, the identification and comprehensive analysis of the MYB gene family in G. lemaneiformis has rarely been studied. In this study, we identified 18 MYB genes in G. lemaneiformis at the genome-wide level, including 15 1R-MYB/MYB-related, 2 R2R3-MYB, and 1 R1R2R3-MYB members. Among them, GlMYB3, GlMYB4, GlMYB8, GlMYB9, and GlMYB15 were remarkably induced under high temperature and were also upregulated by salicylic acid, methyl jasmonate, or abscisic acid. GlMYB3, GlMYB4, GlMYB9, and GlMYB15 were localized in the nucleus when transiently expressed in Nicotiana benthamiana plants. GlMYB4, as one of the most strongly induced high temperature-associated genes, showed transactivation activity, and the C-terminal was critical for the transactivation activity. By yeast two-hybrid screening, GlMYB4 may interact with three candidate proteins: calcineurin subunit B (CNB), O-linked N-acetylglucosamine transferase (OGT), and cleavage and polyadenylation specificity factor (CPSF) to modulate high temperature tolerance.</p>\",\"PeriodicalId\":690,\"journal\":{\"name\":\"Marine Biotechnology\",\"volume\":\"27 4\",\"pages\":\"113\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine Biotechnology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10126-025-10482-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10126-025-10482-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
细叶纤毛藻作为一种具有重要经济价值的红藻,经常遭受高温胁迫,对藻类产量甚至生存造成威胁。培育具有稳定性状和耐热性的新品种至关重要;因此,研究羊草高温响应基因的发现和功能分析具有重要意义。MYB (v-myb禽成髓细胞病病毒癌基因同源)基因家族是真核生物中最大的转录因子超家族之一,已被证明可调节多种环境胁迫。然而,目前对lemaneiformis中MYB基因家族的鉴定和综合分析研究较少。在本研究中,我们在全基因组水平上鉴定了18个MYB基因,包括15个1R-MYB/MYB相关成员,2个R2R3-MYB成员和1个R1R2R3-MYB成员。其中,GlMYB3、GlMYB4、GlMYB8、GlMYB9和GlMYB15在高温下被显著诱导,水杨酸、茉莉酸甲酯和脱落酸也能上调表达。GlMYB3、GlMYB4、GlMYB9和GlMYB15在烟叶中短暂表达时定位于细胞核内。GlMYB4是高温诱导最强烈的基因之一,具有反激活活性,而c端是反激活活性的关键。通过酵母双杂交筛选,GlMYB4可能与钙调磷酸酶亚基B (CNB)、O-linked n -乙酰氨基葡萄糖转移酶(OGT)和裂解与聚腺苷化特异性因子(CPSF) 3种候选蛋白相互作用,调节高温耐受性。
Genome-Wide Identification and Expression Analysis of the MYB Gene Family in Gracilariopsis lemaneiformis to Reveal Potential Members Involved in High-Temperature Stress.
Gracilariopsis lemaneiformis, as an economically important red alga, often suffers high-temperature stress which poses a threat to algal yield and even survival. It is important to achieve new varieties with stable traits and heat tolerance; hence, research on the discovery and functional analysis of high-temperature responsive genes of G. lemaneiformis is significant. MYB (v-myb avian myeloblastosis viral oncogene homolog) gene family is one of the largest transcription factor superfamilies in eukaryotes and has been proved to regulate multiple environmental stresses. However, the identification and comprehensive analysis of the MYB gene family in G. lemaneiformis has rarely been studied. In this study, we identified 18 MYB genes in G. lemaneiformis at the genome-wide level, including 15 1R-MYB/MYB-related, 2 R2R3-MYB, and 1 R1R2R3-MYB members. Among them, GlMYB3, GlMYB4, GlMYB8, GlMYB9, and GlMYB15 were remarkably induced under high temperature and were also upregulated by salicylic acid, methyl jasmonate, or abscisic acid. GlMYB3, GlMYB4, GlMYB9, and GlMYB15 were localized in the nucleus when transiently expressed in Nicotiana benthamiana plants. GlMYB4, as one of the most strongly induced high temperature-associated genes, showed transactivation activity, and the C-terminal was critical for the transactivation activity. By yeast two-hybrid screening, GlMYB4 may interact with three candidate proteins: calcineurin subunit B (CNB), O-linked N-acetylglucosamine transferase (OGT), and cleavage and polyadenylation specificity factor (CPSF) to modulate high temperature tolerance.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.