Screening of Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides from Thermolytic Hydrolysate of Arthrospira platensis

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nur Maulida Safitri, Jue-Liang Hsu
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引用次数: 0

Abstract

Angiotensin-I Converting Enzyme (ACE, EC 3.4.15.1) plays an essential role in controlling blood pressure. In this research, ACE inhibitors extracted from Arthrospira platensis thermolysin protease were provided using various chromatographic techniques, including reversed-phase high-performance liquid chromatography (RP-HPLC) and strong cation exchange chromatography (SCX). The amino acid sequence was determined by liquid-chromatography-tandem mass spectrometry (LC–MS/MS) and identified using two independent approaches: database-assisted identification and de novo sequencing. FY11 (FSESSAPEQHY) and IR5 (ILLYR) were established with the m/z 1281.54 and 677.37, respectively. The IC50 of IR5 from triplicate experiments was lower than FY11, with the value 10.54 ± 1.38 µM. IR5 was regarded as a non-competitive ACE inhibitor, with the docking interaction energy of − 106.842 kJ/mol. Docking results revealed that the interaction between ACE and peptide existed outside the ACE active site, excluding Arg 522, one of the Cl2 binding sites. Notably, the content of IR5 in 2 mg of crude thermolysin digest was determined to be 2.42 µg/mg using LC–MS/MS quantification. Based on all these features, Arthrospira peptides can be considered to be a potentially promising antihypertensive agent.

Graphical Abstract

platarthrospira热解水解液中血管紧张素i转换酶(ACE)抑制肽的筛选
血管紧张素- 1转换酶(ACE, EC 3.4.15.1)在控制血压中起重要作用。本研究采用反相高效液相色谱法(RP-HPLC)和强阳离子交换色谱法(SCX)等多种色谱技术,从platarthrospira thermolysin蛋白酶中提取ACE抑制剂。氨基酸序列采用液相色谱-串联质谱(LC-MS /MS)测定,并采用数据库辅助鉴定和从头测序两种独立的方法进行鉴定。FY11 (FSESSAPEQHY)和IR5 (ILLYR)的m/z分别为1281.54和677.37。三次实验中IR5的IC50值低于FY11,为10.54±1.38µM。IR5是一种非竞争性ACE抑制剂,其对接作用能为−106.842 kJ/mol。对接结果显示ACE与肽的相互作用存在于ACE活性位点之外,不包括Cl2结合位点之一Arg 522。通过LC-MS /MS定量测定,2 mg热溶菌素粗消化物中IR5的含量为2.42µg/mg。基于所有这些特征,节螺旋肽可以被认为是一种潜在的有前途的降压药。图形抽象
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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