Cell Biochemistry and Biophysics最新文献

Thiamine Mitigates the Toxicity of Methylmercury in Cultured Fetal Fibroblast Cell Lines. 硫胺素减轻甲基汞对培养的胎儿成纤维细胞系的毒性。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-21 DOI: 10.1007/s12013-025-01841-z

Parisa Sadighara, Jamileh Salar-Amoli, Abbas Barin, Sher Ali, Carlos Augusto Fernandes Oliveira

{"title":"Thiamine Mitigates the Toxicity of Methylmercury in Cultured Fetal Fibroblast Cell Lines.","authors":"Parisa Sadighara, Jamileh Salar-Amoli, Abbas Barin, Sher Ali, Carlos Augusto Fernandes Oliveira","doi":"10.1007/s12013-025-01841-z","DOIUrl":"https://doi.org/10.1007/s12013-025-01841-z","url":null,"abstract":"The challenge in addressing methylmercury (MeHg) poisoning primarily lies in devising effective therapeutic strategies. In this study, we explore the potential cytoprotective effects of thiamine pyrophosphate (TPP) as a novel agent to alleviate MeHg-induced complications. Fetal fibroblast cells were exposed to 100 µM MeHg with varying concentrations of TPP (12.5-100 mM). Treated and control cells were analyzed for determination of DNA and protein contents, whereas glutathione and lipid peroxidation levels were measured as oxidative stress markers. TPP reduced the cellular lipid peroxidation and restored the intracellular glutathione levels altered by MeHg, also increasing the cell DNA content in the 12.5 mM TPP treatment group. TPP treatment led to enhanced cell survival, underscoring TPP's capacity to alleviate MeHg toxicity by improving the antioxidant status. Further studies on additional oxidative stress markers and apoptotic pathways are necessary to fully elucidate the scope and mechanisms of TPP's cytoprotective effects against MeHg toxicity. While the data in this trial highlight the potential of TPP as a novel therapeutic agent for individuals exposed to MeHg, clinical studies are required to confirm its protective efficacy aiming at developing future mitigation strategies.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Metformin Treatment Against Endometrial Cancer Cells Cultured In Vitro or Grafted into Female Balb/C Nude Mice: Insights into Cell Response and IGF-1R and PI3K/AKT/mTOR Signaling Pathways. 二甲双胍治疗对体外培养或移植到雌性Balb/C裸鼠子宫内膜癌细胞的影响：细胞反应和IGF-1R和PI3K/AKT/mTOR信号通路的见解

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-21 DOI: 10.1007/s12013-025-01840-0

Vânia Marísia Santos Fortes Dos Reis, Franciely Machado Ramos, Henrique Leal de Oliveira, Fernanda Dapper Machado, Sara Hartke, Amanda Machado-Weber, Ariane Germeyer, Thomas Strowitzki, Lúcia Maria Kliemann, Helena von Eye Corleta, Ilma Simoni Brum, Edison Capp, Leo Anderson Meira Martins

{"title":"Effects of Metformin Treatment Against Endometrial Cancer Cells Cultured In Vitro or Grafted into Female Balb/C Nude Mice: Insights into Cell Response and IGF-1R and PI3K/AKT/mTOR Signaling Pathways.","authors":"Vânia Marísia Santos Fortes Dos Reis, Franciely Machado Ramos, Henrique Leal de Oliveira, Fernanda Dapper Machado, Sara Hartke, Amanda Machado-Weber, Ariane Germeyer, Thomas Strowitzki, Lúcia Maria Kliemann, Helena von Eye Corleta, Ilma Simoni Brum, Edison Capp, Leo Anderson Meira Martins","doi":"10.1007/s12013-025-01840-0","DOIUrl":"https://doi.org/10.1007/s12013-025-01840-0","url":null,"abstract":"Obesity and type II diabetes are independent risk factors for Endometrial cancer (EC) development. Elevated levels of insulin-like growth factor-1 (IGF-1), insulin resistance, and the increased activity of IGF-1 receptor is linked to EC development through the PI3K/AKT/mTOR pathway. The antidiabetic agent metformin is a promising repurposing drug for cancer treatment, but the mechanisms underlying its effects are not completely known. This study evaluated how metformin could act against the EC cell line Ishikawa cultured in vitro or grafted into female Balb/C nude mice. In vitro experiments demonstrated that treatment with 25 mM of metformin reduced cell viability through promoting cytotoxicity, mitochondrial dysfunction, apoptosis, and cell cycle arrest (G1 phase). Mice treatment with 250 mg/kg of metformin for 28 days did not change serum IGF-1 levels nor decreased the grafted cell-induced tumor weight and cell proliferation, but prevented its volume growth while genes of the IGF1-R and PI3K/AKT/mTOR pathways (AKT2, GAPDH, FOXO3, IGF1R, INSR, MAPK3, MTOR, and SHC1) were downregulated. Metformin treatment was more impacting for the in vitro model, but our molecular results provide valuable insights into the possible action of metformin against EC tumoral cells at physiological level. In-silico analysis using Cytoscape indicated that metformin was not described as interacting with AKT2 and SHC1 proteins. Besides interacting with metformin, mTOR and MAPK3 present the larger number of interactions with the other proteins. These four genes/proteins emerge as potential targets for deepening studies to determine the metformin's role in longer EC treatment using animal models.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Royal Jelly Enhances the Sensitivity of Oral Squamous Cancer Cells to Paclitaxel, Suppressing Proliferation, Migration, and Glycolysis. 蜂王浆增强口腔鳞癌细胞对紫杉醇的敏感性，抑制增殖、迁移和糖酵解。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-19 DOI: 10.1007/s12013-025-01834-y

Tuğba Kul Köprülü, Bahar Gezer, Jülide Balkan

{"title":"Royal Jelly Enhances the Sensitivity of Oral Squamous Cancer Cells to Paclitaxel, Suppressing Proliferation, Migration, and Glycolysis.","authors":"Tuğba Kul Köprülü, Bahar Gezer, Jülide Balkan","doi":"10.1007/s12013-025-01834-y","DOIUrl":"https://doi.org/10.1007/s12013-025-01834-y","url":null,"abstract":"Royal jelly (RJ) is a natural product that reduces toxic effects and has anti-proliferative effects. The aim of the study is to increase the anticancer effect of Paclitaxel (PAX), which is used in cancer treatment, and to reduce its toxic effect with RJ in oral squamous carcinoma cells. Cytotoxicity tests of RJ and PAX substances were tested on healthy gingival HGF cells and their anti-proliferative effects on UPCI-SCC-131 cells with real-time cell analyzer (xCELLigence RTCA). Their anti-migratory properties were observed with wound healing assay. Glycolysis stress test was performed with Seahorse XFe24 to measure the glycolytic capacity. Total RNA-seq libraries were created and sequenced with NovaSeq 6000. Transcriptome profiles were created with bioinformatic analyses and functional enrichment analyses were performed. Results demonstrate that both RJ and PAX exhibit significant anti-proliferative effects against oral squamous cell carcinoma cells, as quantified by real-time cell analysis. Notably, RJ co-treatment mitigated PAX-induced cytotoxicity in healthy human gingival fibroblasts, suggesting a protective role against chemotherapy-associated toxicity. While both compounds inhibited cancer cell proliferation, PAX particularly displayed potent anti-migratory properties in wound healing assays, significantly impairing OSCC cell motility. Metabolic profiling revealed that the RJ-PAX combination therapy substantially reduced glycolytic capacity in OSCC cells, indicating disruption of their energy metabolism. Transcriptomic analysis identified downregulation of critical cell cycle regulators (MCM2, CDC25A, CCNE2) and DNA replication factors (RFC2, PCNA), along with modulation of MYC and E2F pathways, providing insights into the observed anti-cancer effects.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bryophyllum pinnatum (L.) Pers. Modulates Multiple Neuroprotective Targets in Alzheimer's Disease: Evidence from Computational and Experimental Validation. 青叶苔藓（L.）珀耳斯。调节阿尔茨海默病中的多个神经保护靶点：来自计算和实验验证的证据。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-19 DOI: 10.1007/s12013-025-01832-0

Laxmi Pattanashetti, Manoj M Donagannavar, Divya Jigalur, Vishal S Patil

{"title":"Bryophyllum pinnatum (L.) Pers. Modulates Multiple Neuroprotective Targets in Alzheimer's Disease: Evidence from Computational and Experimental Validation.","authors":"Laxmi Pattanashetti, Manoj M Donagannavar, Divya Jigalur, Vishal S Patil","doi":"10.1007/s12013-025-01832-0","DOIUrl":"https://doi.org/10.1007/s12013-025-01832-0","url":null,"abstract":"Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, with limited therapeutic options and adverse effects associated with long-term pharmacological treatments. This study investigated the neuroprotective potential of Bryophyllum pinnatum (B. pinnatum) through integrative in silico and in vivo approaches. Network pharmacology and pathway enrichment analyses (KEGG, Cytoscape 3.10.1) were used to identify compound-target network association. Molecular docking using AutoDock Vina and molecular dynamics (MD) simulations for 200 ns using GROMACS were executed to assess the stability of the key ligands and targets. Cognitive impairment was induced in Wistar rats using scopolamine (1 mg/kg, i.p.). Animals were treated with B. pinnatum hydroalcoholic leaf extract (200 and 400 mg/kg, p.o.) and donepezil (3 mg/kg, i.p.) for 30 days. Cognitive and motor functions were evaluated via Morris water maze, elevated plus maze, locomotor activity, and grip strength tests. Biochemical assays measured acetylcholinesterase (ACHE) activity, β-amyloid (Aβ) levels, glutathione, and lipid peroxidation. Histopathological analysis of brain tissue assessed neuronal integrity. In silico analyses identified multiple phytoconstituents involved in AD-relevant pathways, including MAPK, PI3K-Akt, and cholinergic signaling. Diosmin exhibited high binding affinities to ACHE (-10.3 kcal/mol) and MAO-B (-11.2 kcal/mol), with stable binding confirmed via MD simulations. In vivo, B. pinnatum significantly improved cognitive performance, motor coordination, and antioxidant status while reducing Aβ aggregation and ACHE activity (p < 0.05). Histological findings showed reduced neuronal degeneration and neuroinflammation. These results highlight the multitarget neuroprotective potential of B. pinnatum, with diosmin emerging as a promising plant-derived candidate for AD therapeutics.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miRNA Landscape of Leukemia - Cellular Actions to Therapy through Molecular Mechanisms. 白血病的miRNA景观-细胞通过分子机制对治疗的作用。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-18 DOI: 10.1007/s12013-025-01820-4

Kang Zi Khor, Adam Azlan, Julia Joseph, Jeyrubini Ramesh, Maheswaran Solayappan, Yee Yik Mot, Mohamed Saleem, Narazah Mohd Yusoff, Emmanuel Jairaj Moses

{"title":"The miRNA Landscape of Leukemia - Cellular Actions to Therapy through Molecular Mechanisms.","authors":"Kang Zi Khor, Adam Azlan, Julia Joseph, Jeyrubini Ramesh, Maheswaran Solayappan, Yee Yik Mot, Mohamed Saleem, Narazah Mohd Yusoff, Emmanuel Jairaj Moses","doi":"10.1007/s12013-025-01820-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01820-4","url":null,"abstract":"The Nobel Prize in Physiology and Medicine awarded to Victor Ambros and Gary Ruvkun for their work in microRNA (miRNA) emphasized the important role that miRNA plays in biology. These conserved small noncoding RNA molecules play pivotal role especially in the post transcriptional stage of gene regulation, affecting various key cellular processes by interacting with target genes complement to the conserved miRNA seed sequence. Over the years miRNA was implicated in all manners of diseases with abnormal gene regulation especially in cancer. In leukemia, miRNA dysregulation could lead to aberrant cellular processes. These include but are not limited to leukemic development, survival and drug resistance. miRNA also have important clinical implications as differential miRNA abundance could contribute towards leukemogenesis. This is especially useful in the diagnosis and prognosis of leukemia. This review aims to explore and highlight the role of miRNAs in leukemia development, progression and to discuss miRNA involvement towards leukemic resistance to therapy.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCBP2 Promotes NRG4 mRNA Stability to Diminish Angiotensin II-Induced Hypertrophy, NLRP3 Inflammasome Activation, and Oxidative Stress of AC16 Cardiomyocytes. PCBP2促进NRG4 mRNA稳定性，减少血管紧张素ii诱导的肥大、NLRP3炎性体激活和AC16心肌细胞的氧化应激。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-18 DOI: 10.1007/s12013-025-01819-x

Yang Zhang, Yulong Liu, Yaling Wang

{"title":"PCBP2 Promotes NRG4 mRNA Stability to Diminish Angiotensin II-Induced Hypertrophy, NLRP3 Inflammasome Activation, and Oxidative Stress of AC16 Cardiomyocytes.","authors":"Yang Zhang, Yulong Liu, Yaling Wang","doi":"10.1007/s12013-025-01819-x","DOIUrl":"https://doi.org/10.1007/s12013-025-01819-x","url":null,"abstract":"Myocardial hypertrophy, a complex cardiovascular disorder, remains a significant challenge. NRG4 has shown protective effects against myocardial damage. Here, we clarified the role of NRG4 in angiotensin II (Ang II)-induced hypertrophy of AC16 cardiomyocytes. The Ang II-stimulated AC16 cell line was used as an in vitro model of myocardial hypertrophy. Immunofluorescence using an anti-α-actinin antibody was used to observe cell area and size. mRNA expression was detected by quantitative PCR, and protein levels were measured by immunoblot assay. ROS amount detection was performed by flow cytometry. The cell protein/DNA ratio and the levels of IL-1β, IL-18, MDA and SOD were tested using commercial kits. The relationship between PCBP2 and NRG4 mRNA was validated by luciferase, RNA immunoprecipitation (RIP), and mRNA stability assays. In Ang II-stimulated AC16 cells, PCBP2 and NRG4 were markedly downregulated. Increased NRG4 expression relieved Ang II-induced hypertrophy and fibrosis in AC16 cardiomyocytes. Moreover, NRG4 increase weakened NLRP3 inflammasome activation and oxidative stress in Ang II-stimulated AC16 cardiomyocytes. Mechanistically, PCBP2 stabilized NRG4 mRNA to increase NRG4 protein expression in Ang II-induced AC16 cardiomyocytes. NRG4 depletion counteracted the suppressive effects of PCBP2 upregulation on hypertrophy, NLRP3 inflammasome activation, and oxidative stress of Ang II-induced AC16 cardiomyocytes. Additionally, the PCBP2/NRG4 cascade regulated the AMPK/mTOR signaling pathway in Ang II-induced AC16 cardiomyocytes. Our data demonstrate that the previously uncharacterized PCBP2/NRG4 cascade attenuates Ang II-triggered hypertrophy, NLRP3 inflammasome activation, and oxidative stress of AC16 cardiomyocytes.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the Adenylate Kinase Activity are Proportional to the ADP/ATP Ratio Upon Resorption and Regeneration of Chlamydomonas reinhardtii Flagella. 莱茵衣藻鞭毛吸收再生时腺苷酸激酶活性的变化与ADP/ATP比值成正比。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-18 DOI: 10.1007/s12013-025-01825-z

Raza Ali Jafri, Yash Raj, Jacinta S D'Souza

{"title":"Changes in the Adenylate Kinase Activity are Proportional to the ADP/ATP Ratio Upon Resorption and Regeneration of Chlamydomonas reinhardtii Flagella.","authors":"Raza Ali Jafri, Yash Raj, Jacinta S D'Souza","doi":"10.1007/s12013-025-01825-z","DOIUrl":"https://doi.org/10.1007/s12013-025-01825-z","url":null,"abstract":"Adenylate kinases (ADK) maintain cellular energy homeostasis and catalyse a reversible reaction that converts two molecules of ADP into ATP and AMP. ATP in Chlamydomonas reinhardtii flagella is utilised by dynein to generate flagellar beating. ATP must be constantly supplied and maintained; however, the constricted nature of flagella restricts the localisation of mitochondria in vicinity. We show that C. reinhardtii flagella carry conserved ADK domain-containing proteins that are large in number and longer than their cytosolic counterparts. Six of the eight flagellar ADKs are enriched in the central pair apparatus (CPA). Upon flagellar regeneration and resorption, the ADK activity changes, suggesting a shift in the energy demands for the two processes. The total ADK activity in regenerating flagella increased, and resorbing flagella showed an equal but reverse effect. ADKs help regenerate ATP locally and act as phosphotransfer agents that spatially direct the transfer of nucleotides. The ADP to ATP ratio during reflagellation and resorption suggests a role for ADKs in maintaining the nucleotide levels. To the best of our knowledge, this is the first study providing evidence for the role of ADK domain-containing proteins in maintaining ATP homeostasis in response to flagella regeneration and resorption.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico and In Vitro Analysis of Bioactive Compounds from Ficus benghalensis as a Novel Approach to Targeting Brain Abscess Pathogen. 红ficus benghalensis生物活性物质作为脑脓肿病原菌的新途径的体外和体外分析。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-17 DOI: 10.1007/s12013-025-01823-1

Ihtesham Arshad, Maryum Zainab, Ayesha Farooq, Saira Zulfiqar, Anna Ali, Rubina Bibi, Shaista Shafiq, Imran Zafar, Muhammad Mazhar Ayaz, Yahya A Almutawif, Najeeb Ullah Khan

{"title":"In Silico and In Vitro Analysis of Bioactive Compounds from Ficus benghalensis as a Novel Approach to Targeting Brain Abscess Pathogen.","authors":"Ihtesham Arshad, Maryum Zainab, Ayesha Farooq, Saira Zulfiqar, Anna Ali, Rubina Bibi, Shaista Shafiq, Imran Zafar, Muhammad Mazhar Ayaz, Yahya A Almutawif, Najeeb Ullah Khan","doi":"10.1007/s12013-025-01823-1","DOIUrl":"https://doi.org/10.1007/s12013-025-01823-1","url":null,"abstract":"The management of brain abscesses, particularly with Escherichia coli (E. coli) in immunocompromised patients, remains contentious. This study evaluates the bioactive potential of Ficus benghalensis extracts against brain abscess pathogens, including multidrug-resistant E. coli and Staphylococcus aureus (S. aureus), through phytochemical, pharmacological, and computational analyses. High yields (75-85%) were obtained from polar solvent extraction (acetone, methanol, ethyl acetate). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 30 bioactive compounds, including myricetin, naringenin-7-O-rutinoside, and harpagoside. Methanol extracts exhibited potent antimicrobial activity with inhibition zones of 18.2 mm (E. coli) and 17.9 mm (S. aureus) and minimum inhibitory concentration (MIC) values of 210-250 µg/mL. Ex vivo assays on clinical isolates showed dose-dependent inhibition (MIC50 = 150 µg/mL). Molecular docking indicated quercetin (-7.5 kcal/mol) and kaempferol (-7.8 kcal/mol) targeting E. coli FimH and OmpA, while lupeol (-9.1 kcal/mol) and ellagic acid (-8.7 kcal/mol) targeted S. aureus PBP2a and Hla. Pharmacokinetic analysis revealed quercetin and gallic acid as lead candidates with 100% gastrointestinal (GI) absorption and bioavailability (0.55-0.56), but limited blood-brain barrier (BBB) permeability (brain score: 0.24). Three-dimensional quantitative structure-activity relationship (3D-QSAR) comparative molecular field analysis (CoMFA) models (R2 = 0.111, Q2 = 0.00) emphasized steric and electrostatic interactions (84%) in bioactivity. These findings suggest that Ficus benghalensis holds potential as a multitarget antimicrobial agent for brain abscess therapy, with further optimization for central nervous system (CNS) delivery needed.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Schiff Bases Derived from 2-hydroxybenzaldehyde as Potential Anticancer Agents: Synthesis, Characterization, Molecular Docking and in-vitro Evaluation. 2-羟基苯甲醛希夫碱作为潜在抗癌药物的探索：合成、表征、分子对接及体外评价

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-16 DOI: 10.1007/s12013-025-01826-y

Oğuzhan Karaosmanoğlu, Halil Berber, Hülya Sivas, Ulku Dilek Uysal

{"title":"Exploring Schiff Bases Derived from 2-hydroxybenzaldehyde as Potential Anticancer Agents: Synthesis, Characterization, Molecular Docking and in-vitro Evaluation.","authors":"Oğuzhan Karaosmanoğlu, Halil Berber, Hülya Sivas, Ulku Dilek Uysal","doi":"10.1007/s12013-025-01826-y","DOIUrl":"https://doi.org/10.1007/s12013-025-01826-y","url":null,"abstract":"Chemotherapy resistance is a major obstacle in cancer; thus, the development of new anticancer agents is being studied with great importance. Schiff bases containing an azomethine group have been widely used in various pharmaceutical applications. Here, five Schiff base derivatives of 2-hydroxybenzaldehyde (8S1-8S5) were synthesized and characterized. Their cytotoxicity was screened against six cancerous and two normal cell lines using a neutral red uptake assay. The apoptotic mechanism of the compound 8S3 in MCF-7 cells was evaluated through a combination of cell cycle analysis, measurement of mitochondrial membrane potential, reactive oxygen species levels, caspase activity, analysis of gene expression in the mitogen-activated protein kinase (MAPK) signalling pathway, and in silico analysis studies. Treatment of the cells with 8S3 resulted in an increase in sub-G0/G1 populations and MMP disruption. The expressions of 30 genes involved in MAPK signalling were upregulated, while 10 genes were downregulated in these cells. This transcriptional profile supports a MAPK-mediated apoptotic response and highlights 8S3's potential to overcome chemoresistance by disrupting key survival MAPK signalling pathways. 8S3 might induces apoptosis through a mechanism involving modulation of MAPK pathway and mitochondrial dysfunction, in a ROS-independent manner. Results highlight 8S3's potential as a candidate for further development of anti-cancer drugs in cancer therapy.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Insights into Oxidative Stress in Colon Cancer Patients. 结肠癌患者氧化应激的生化分析。

IF 1.8 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-07-16 DOI: 10.1007/s12013-025-01827-x

Seerwan Hamadameen Sulaiman, Hoshyar Saadi Ali, Rebaz Anwar Omer, Hemn A H Barzani, Musher Ismael Salih, Aryan Fathulla Qader

{"title":"Biochemical Insights into Oxidative Stress in Colon Cancer Patients.","authors":"Seerwan Hamadameen Sulaiman, Hoshyar Saadi Ali, Rebaz Anwar Omer, Hemn A H Barzani, Musher Ismael Salih, Aryan Fathulla Qader","doi":"10.1007/s12013-025-01827-x","DOIUrl":"https://doi.org/10.1007/s12013-025-01827-x","url":null,"abstract":"The purpose of this research was to assess oxidative stress levels in colon cancer patients and examine their association with disease onset and progression. 176 individuals were recruited, comprising 106 colon cancer patients and 70 healthy controls. Serum oxidative stress marker levels of protein carbonyl (PCO), ischemia-modified albumin (IMA), malondialdehyde (MDA), and glutathione-S-transferase (GST) and antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were quantified. The tumor markers carcinoembryonic antigen (CEA) and cancer antigen 19-9 (CA 19.9) were also evaluated. The levels of PCO, IMA, MDA, and GST were significantly increased (P < 0.01 for each) with a significant decrease in GPX and SOD levels (P < 0.01) when compared to the control group. No significant difference was noted in CAT levels. The tumor markers CEA and CA 19.9 were significantly increased in the patient group (P < 0.01). These results suggest an imbalance of oxidative/antioxidant status in favor of oxidative stress in patients with colon cancer. The study identifies oxidative stress as a major factor in the pathogenesis of colon cancer. Clinically, biomarkers such as IMA with more than 80% sensitivity can be powerful secondary aids to early detection or monitoring disease progression. The findings suggest that modulating oxidative stress would be therapeutic in the treatment of colon cancer.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0