Cell Biochemistry and Biophysics最新文献

{"title":"Syringic Acid in Canarium odontophyllum for Diabetes and Obesity - A Review.","authors":"Gilbert Ringgit, Bo Eng Cheong, Muhammad Dawood Shah, Noor Atiqah Aizan Abdul Kadir, Shafiquzzaman Siddiquee","doi":"10.1007/s12013-025-01773-8","DOIUrl":"https://doi.org/10.1007/s12013-025-01773-8","url":null,"abstract":"<p><p>Syringic acid (SA) is a phenolic compound with a significant role in the treatment of diabetes and obesity. Syringic acid possesses anti-obesity and anti-diabetic properties; however, the potential of syringic acid derived from the native Bornean fruit Canarium odontophyllum (C. odontophyllum) for managing diabetes and obesity remains undocumented. This brief discussion explores the possible mechanisms associated with syringic acid's structure and its potential therapeutic effects in managing diabetes and obesity. The relevant information is gathered from previous reports on syringic acid, related to molecular docking studies involving syringic acid-associated enzymes and protein residues. The potential mechanism of syringic acid derived from C. odontophyllum with chemical structure characterized by a benzene ring with hydrogen bonds and its high affinity for enzymes and protein residues targeting diabetes and obesity, including hexokinase 2 (HK2), glycogen synthase kinase (GSK), 2BEL, protein kinase D (PKD), insulin receptor substrate-1 (IRS-1), and insulin receptor beta (IR-β). This review paper provides alternative insights into syringic acid derived from the seasonal fruit of native Bornean fruit associated with molecular docking, structural advantages and mechanism of action in diabetes treatment.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Potent Plant-Derived Chitinase: Structural Informatics and Insecticidal Activity against Helicoverpa armigera.","authors":"Shah Rukh, Ahmed Akrem, Qamar Saeed, Sohaib Mehmood, Nosherwan Adil, Tazeen Rao, Muhammad Nasir, Uzma Ishaq, Aqal Zaman, Najeeb Ullah, Mohibullah Shah","doi":"10.1007/s12013-025-01777-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01777-4","url":null,"abstract":"<p><p>Helicoverpa armigera (cotton bollworm) is a globally distributed lepidopteran pest that causes estimated annual agricultural losses exceeding 5 billion USD. While chemical pesticides remain the primary control strategy, their prolonged use has led to significant environmental contamination, development of widespread insecticide resistance, and non-target organism toxicity. These limitations underscore the critical need for plant-derived biopesticides that offer target specificity, environmental biodegradability, and sustainable pest management solutions without promoting resistance development. Here, we elucidate the insecticidal potential of Nelumbo nucifera Chitinase (NnChi) against the insect H. armigera through structural informatics and in-vivo insecticidal bioassays. SDS-PAGE showed a single band of ~32 kDa, and LC-MS/MS analysis depicted a fragment of 10 amino acids with 100% identity with Family 19 Class I Chitinase of Mangifera indica. NnChi-predicted structure revealed its two domains (ChB D, Cat D) connected through linker region and docking analysis of both these domains with (GlcNAc)₄ showing binding affinities of -5.6 kcal/mol and -7.0 kcal/mol, respectively. MD simulation (100 ns) showed that 4 residues (RQCR) of ChB D and 4 residues (NRIP) of Cat D contributed to binding with (GlcNAc)₄. To the best of our knowledge, we are reporting the molecular interactions of both domains (ChB D and Cat D) with (GlcNAc)₄ via simulation studies for the first time. These computational findings were further verified through insecticidal assay. Significant larval mortality of H. armigera was observed from 3^rd-6^th instar against 15 µg/g NnChi treatment. Among life cycle parameters, larval and pupal duration, adult eclosion, larval and pupal weight are significantly decreased in a concentration-dependent manner as compared to control. Our integrated structural-functional characterization demonstrates that NnChi exhibits significant insecticidal activity against Helicoverpa armigera. These findings establish NnChi as a promising biopesticide candidate worthy of further investigation.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Turmeric on Manganese-induced Redox Imbalance in Testicular Tissues: Histo-micrometric and Biochemical analyses.","authors":"Fatima Bashir Ahmad, Chaman Ara, Shaukat Ali, Shagufta Andleeb, Hafiz Abdullah Shakir, Faheem Nawaz, Asmatullah","doi":"10.1007/s12013-025-01763-w","DOIUrl":"https://doi.org/10.1007/s12013-025-01763-w","url":null,"abstract":"<p><p>Manganese is the most overlooked chemical element, though it is the 5^th most abundant metal in earth' crust. Despite being important in the body's mechanisms, bioaccumulation of manganese in living organisms can be noxious. The current study aimed to evaluate the effects of manganese on male mice's reproductive parameters, and turmeric was used as a remedial agent against manganese-induced potential subchronic toxicity. Healthy male mice were distributed into seven groups (n = 10), Control (untreated), Vehicle control; VC-I (0.1 ml of saline solution), Mn-I (5 mg/Kg BW), Mn-II (10 mg/Kg BW), Mn-III (20 mg/Kg BW), Mn + T (20 mg of Mn/Kg +100 mg turmeric/Kg BW) and Vehicle control; VC-II (0.1 ml of olive oil) via oral gavage routinely, once a day. The experiment was continued for 28 days. Before administrations, the antioxidant capacity of turmeric was evaluated via FRAP, TPC & GC-MS assays. Mice were acclimatized for 10 days after dosing, then euthanized and samples (testis & blood) were recovered. Morphological observations showed minute morphological changes in testes as compared to controls. Morphometric analysis revealed average body weight, testis weight and size of Mn-intoxicated mice were reduced remarkably (P ≤ 0.05) in comparison with the control group. Sideways Mn + T group showed non-significant changes in both parameters. Hematological, micrometric findings and serum testosterone, luteinizing hormone and follicle stimulating hormone were significantly different (P ≤ 0.05) in Mn-exposed groups against controls. These alterations were concomitant with histological variations in Mn-treated groups. While such deviations were less obvious in the Mn + T-administered group. The aforesaid findings deduced that the turmeric supplementation manifested improvements in most of the histo-micrometric, hematological, steroidal parameters and enzymatic indices of mice through its antioxidant action.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Icariside II Alleviates Chondrocyte Inflammatory Injury by Inhibiting the TNIP2/NF-κB Pathway.","authors":"Jiajun Mei, Hongkui Ke, Junsong Zhu","doi":"10.1007/s12013-025-01769-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01769-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palmitic Acid-induced Renal Injury: Unravelling the Molecular Mechanisms and the Protective Role of Lycopene.","authors":"Adewale S James, Emmanuel I Ugwor, Adio J Akamo, Dorcas I Akinloye, Ayokulehin M Kosoko, Boluwatife A Olagunju, Emmanuel O Ezenandu, Charity C Amaogu, Victoria Adebiyi, Funmilola C Thomas, Regina N Ugbaja","doi":"10.1007/s12013-025-01775-6","DOIUrl":"https://doi.org/10.1007/s12013-025-01775-6","url":null,"abstract":"<p><p>To understand the role of palmitic acid overload on renal physiology, we exposed female rats to palmitic acid (PA) beyond the physiological range reminiscent of a high-fat western diet. We then treated the rats with graded doses of lycopene (Lyc) to evaluate its potential remedial effect against PA-induced renal injury. Twenty-four rats were randomized into four groups as control (received vehicles; Free Fatty Acid Free Bovine Serum Albumin (BSA) and Olive oil; PA only [received 5 mM BSA-complexed PA]; while the other two groups received 10 and 20 mg/kg body weight of Lyc along with the BSA-complexed PA. The Olive oil-reconstituted Lyc was commenced at the seventh week of intraperitoneal PA injection (uninterrupted) until the nineth week. Our results show that palmitic acid PA overload caused renal lipid dystrophy, characterized by decreased cholesterol, triglycerides, and phospholipids. We also observed elevated activities of lactate dehydrogenase and decreased activity of alanine aminotransferase. The activities of superoxide dismutase, myeloperoxidase, and malondialdehyde levels increased, while glutathione peroxidase activity decreased significantly. Furthermore, PA-induced disruption of cellular electrolytes is characterized by decreased calcium, chloride, sodium, and magnesium ions, and elevated activities of Na⁺/K⁺, and Ca²⁺/Mg²⁺ ATPases. Western blot analysis shows decreased Nrf2 expression, while NF-^KB and Toll -Like Receptor 4 (TLR4) increased. Furthermore, the mRNA expression of TLR4, IL-6, TNF-alpha, and IL-1β increased, while IL-10 decreased in PA only group. However, Lyc treated groups exhibits meaningful ameliorative potentials by abating oxidative stress, inflammation, lipid dystrophy, and iono-dysregulation. This study suggests lycopene supplementation might abate PA -invoked disruption of lipid metabolism, inflammatory signal propagation and disruption of innate antioxidant systems in female albino rats.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micronized Purified Flavonoid Fraction (Diosmin/Hesperidin) Ameliorates Cardiac Structural and Functional Integrity in Cisplatin-treated Male Wistar Rats by Modulating NLRP3/Caspase-1/-3 Signaling.","authors":"W A Saka, P A Oyedokun, C A Adegbola, T M Akhigbe, P J Ashonibare, O R Kolawole, A A Oladipo, R E Akhigbe","doi":"10.1007/s12013-025-01774-7","DOIUrl":"https://doi.org/10.1007/s12013-025-01774-7","url":null,"abstract":"<p><p>Cisplatin is an effective chemotherapeutic agent in managing several cancers. Yet, its usage is restricted by its toxicity to non-target organs, such as cardiotoxicity that is mediated by nucleotide-binding Oligomerisation Domain (NOD)-Like Receptors family pyrin domain containing 3 (NLRP3)-driven inflammation, oxidative stress, and apoptosis. Conversely, micronized purified flavonoid fractions (MPFF) attenuate oxido-inflammation by downregulating NLRP3 inflammasome. However, there is a dearth of information on the effect of MPFF on cisplatin-induced cardiac injury. This study examined the possible protective effect of MPFF in cisplatin-induced cardiac injury. Also, the role of NLRP3 inflammasome and caspase-1/-3 signaling was evaluated. Thirty-two adult male Wistar rats were randomly allotted to four equal groups (n = 8 rats per group). The control received 0.5 mL of distilled water orally daily, the MPFF-treated rats received 100 mg/kg/day of MPFF orally for 14 days, the cisplatin-treated rats had 7 mg/kg of cisplatin via an intraperitoneal route on day 8, and the cisplatin+MPFF -treated rats received cisplatin and MPFF as those in the cisplatin- and MPFF-treated groups. Cisplatin therapy significantly increased cardiac injury markers and plasma glucose. Cisplatin also induced dyslipidemia and insulin resistance. Moreover, cisplatin altered cardiac histology evidenced by vascular congestion, and increased myofibril thickness and interstitial space. These observations were accompanied by cisplatin-induced cardiac oxidative stress (increased malondialdehyde and a decline in reduced glutathione, superoxide dismutase, and catalase), inflammation (increased tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6), apoptosis (increased caspase 1 and caspase 3) and a marked increase in NLPR3 inflammasome. These derangements were blunted by MPFF co-therapy. In conclusion, this study for the first time demonstrated that MPFF attenuated cisplatin-induced cardiac structural and functional damage by suppressing oxidative stress and inflammation via the downregulation of NLPR3 /caspase-1/-3 signaling.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Paeonol of Total Glucosides of White Peony Regulates the Differentiation of CD4+Treg Cells through the EP300/Foxp3 Axis to Relieve Pulmonary Fibrosis in Mice.","authors":"Muyun Yan, Qing Wang, Hongzhong Yang, Da Liu, Weijun Liang, Huamei Chen","doi":"10.1007/s12013-025-01770-x","DOIUrl":"https://doi.org/10.1007/s12013-025-01770-x","url":null,"abstract":"<p><p>Pulmonary fibrosis is a chronic progressive lung disease that can lead to lung structural damage and respiratory failure. This study aimed to investigate whether paeonol could improve pulmonary fibrosis in mice by regulating through the EP300/Foxp3 axis. We established a mouse model of pulmonary fibrosis. Total glucosides of white peony (TGP) were used to treat the animal model, and lung injury was observed using HE and Masson staining. Inflammatory factor levels in bronchoalveolar lavage fluid were detected using ELISA. Network pharmacology analysis was conducted to explore the components, shared targets, and signaling pathways of pulmonary fibrosis and TGP. Molecular docking was performed to observe the binding of paeonol, a component of TGP, to the target EP300. CD4+T cells were collected and co-cultured with MPPF cells, followed by intervention with TGP and paeonol. The ratio of CD4+T/Treg cells was measured in vitro, and immunofluorescence was used to detect the intensity of α-SMA. Network pharmacology revealed that one of the key components of TGP is paeonol, and the signaling pathway associated with pulmonary fibrosis is the Foxp3 signaling pathway. TGP effectively inhibited the secretion of lung inflammatory factors TGF-β1, IFN-γ, IL-2, and IL-17 in mic. Paeonol, an effective component of TGP, could bind to EP300 at the molecular level. Both TGP and paeonol inhibited the expression of EP300 in CD4+T and MPPF cells, enhanced the proportion of Treg cells in CD4+T, and reduced the expression of Collagen I and α-SMA in MPPF cells. TGP can effectively inhibit lung inflammation and fibrosis progression in mice. Paeonol regulating CD4+Treg cell differentiation through the EP300/Foxp3 axis. This study may provide new insights into how TGP improves pulmonary fibrosis in mice.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NanoBiT-based Analysis of Canine SOD1 Protein Dynamics: Understanding the Role of CCS and Ebselen Derivatives as Potential Therapeutics for Canine Degenerative Myelopathy.","authors":"Sakura Hirose, Yui Kobatake, Norihiro Tada, Mahmoud Kandeel, Akichika Itoh, Kentaro Oh-Hashi","doi":"10.1007/s12013-025-01768-5","DOIUrl":"https://doi.org/10.1007/s12013-025-01768-5","url":null,"abstract":"<p><p>Canine degenerative myelopathy (DM) is a progressive neurodegenerative disorder that shares common pathological features with amyotrophic lateral sclerosis (ALS) in humans. Both diseases are linked to mutations in the superoxide dismutase 1 (SOD1) gene. Understanding the molecular differences between wild-type (WT) and mutant SOD1 proteins is critical for developing therapeutic strategies. In this study, we employed the NanoLuc complementation (NanoBiT) reporter system to investigate the expression and functional differences between WT and E40K mutant canine SOD1 to assess the therapeutic potential of copper chaperone for SOD1 (CCS) and ebselen derivatives. E40K cSOD1 displayed significantly reduced luciferase activity compared to WT cSOD1 in all NanoBiT-tagged combinations, indicating altered homodimerization and protein stability. Co-transfection with CCS increased both WT and mutant cSOD1 protein levels and reporter activities, with a more pronounced effect on the E40K mutant. Ebselen treatment enhanced luciferase activity, particularly in E40K cSOD1-expressing cells. Two compounds (compounds 2 and 5) were stronger than the parent compound in improving mutant cSOD1-derived NanoBiT activities. Additionally, molecular docking simulations revealed stronger binding affinities of ebselen and its derivatives to E40K cSOD1, suggesting potential therapeutic benefits. In conclusion, the NanoLuc reporter system offers a valuable tool for screening potential therapeutics for SOD1-linked neurodegenerative diseases. CCS and ebselen derivatives exhibited promising effects on SOD1 activity, providing a basis for future therapeutic strategies targeting both DM and ALS.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycyrrhiza pallidiflora Polysaccharide Ameliorates DSS-Induced Colitis by Protecting Intestinal Barrier Integrity.","authors":"Dandan Zeng, Weijie Ren, Bo Zhao, Yuanyuan Li, Jinlong Jiao, Tianlu Mo","doi":"10.1007/s12013-025-01765-8","DOIUrl":"https://doi.org/10.1007/s12013-025-01765-8","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease. Glycyrrhiza pallidiflora polysaccharide (GPP) is an important constituent of a species of Glycyrrhiza pallidiflora, but its therapeutic mechanism in UC mice is not clear. A dextran sulphate sodium salt (DSS)-induced mouse model of UC was established, and GPP was extracted by ultrasound-assisted extraction, optimised to a GPP content of 25.66% by one-factor optimisation. The effects of different doses (100, 200, 300 mg/kg) of GPP on UC were investigated. The results showed that GPP could delay the trend of weight loss, reduce the DAI score and decrease colon damage in mice, and GPP had a better ameliorative effect on enteritis, which provided a theoretical basis for studying the effect of natural products on UC.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Phytochemical, Antibacterial, and Hepatoprotective Effects of Elaeagnus umbellata Leaf Extract Against Liver Injury in an Animal Model.","authors":"Nausheen Nazir, Sajid Khan, Nasiara Karim, Mohammad Nisar, Tariq Aziz, Ashwag Shami, Fahad Al-Asmari, Areej A Alhhazmi, Fakhria A Al-Joufi, Maher S Alwethaynani","doi":"10.1007/s12013-025-01767-6","DOIUrl":"https://doi.org/10.1007/s12013-025-01767-6","url":null,"abstract":"<p><p>Elaeagnus umbellata, commonly known as autumn olive, is considered a medicinal plant of high value and belongs to the Elaeagnaceae family. It exhibits anti-ulcer, antimutagenic, antimicrobial, and neuroprotective properties. The leaves of E. umbellata reportedly have pharmacological activities, including antibacterial, anti-inflammatory, and anticancer effects. However, no in vivo studies have evaluated this plant's hepatoprotective potential. In this study, the hepatoprotective potential of E. umbellata was determined using an in vivo model. Extraction from the leaves of E. umbellata was carried out using standard methods, which then were subjected to gas chromatography and mass spectroscopy for characterization. Fourteen compounds were identified in the crude methanolic extract (Met-Ext) of E. umbellata leaves. Total phenolic and total flavonoid contents were assessed, and the extract was tested for hepatoprotective potential against carbon tetrachloride (CCL₄)-induced liver injury using rats as an experimental model. The samples exhibited antibacterial potential against bacterial strains such as Pseudomonas Aeruginosa 25619 (25 mm zone of inhibition) and Enterococcus faecalis 29212 (26 mm zone of inhibition). No inhibition was noted for Klebsiella pneumonia 43816 relative to the standard imipenem (34 mm zone of inhibition on average). A marked increase was observed in the levels of some serum liver biochemical parameters such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total cholesterol, serum creatinine, total serum bilirubin, total triglyceride, and low-density lipoprotein. By contrast, a reduction was observed in other parameters, such as high-density lipoprotein, in a group of animals treated with CCl₄. The extract possessed substantial protective properties against CCl₄-induced liver toxicity, thereby mitigating liver damage and restoring liver function. The results of this in vivo study indicate that the crude Met-Ext of E. umbellata leaves exhibits considerable hepatoprotective effects in a dose-dependent manner.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}