Environmental Microbiology Reports最新文献

Bacterial Communities From Two Freshwater Aquaculture Systems in Northern Germany

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-15 DOI: 10.1111/1758-2229.70062

Júlia Clols-Fuentes, Julien A. Nguinkal, Patrick Unger, Bernd Kreikemeyer, Harry W. Palm

{"title":"Bacterial Communities From Two Freshwater Aquaculture Systems in Northern Germany","authors":"Júlia Clols-Fuentes, Julien A. Nguinkal, Patrick Unger, Bernd Kreikemeyer, Harry W. Palm","doi":"10.1111/1758-2229.70062","DOIUrl":"10.1111/1758-2229.70062","url":null,"abstract":"The microbial communities in aquaculture systems are primarily affected by changes in water quality, fish metabolism, feeding strategies and fish disease prevention treatments. Monitoring changes in aquatic microbiomes related to aquaculture activities is necessary to improve management strategies and reduce the environmental impact of aquaculture water discharge. This study assessed the effects of activities within two fish farms on water microbiome composition by analysing the water entering and leaving both systems. Additionally, pathogenic bacterial species associated with common fish diseases were identified. The abundance, diversity and identity of microorganisms were evaluated using 16S rRNA hypervariable gene region amplicon sequencing. Proteobacteria (38.2%) and Bacteroidetes (31.3%) were the most abundant phyla in all water samples. Changes in microbiome composition after passage through the fish tanks were observed in several taxa, such as Nitrospirae, Chloroflexi, Deferribacteres and Cyanobacteria. Flavobacterium sp. and Pseudomonas sp. were the predominant potential pathogens and heterotrophic bacteria detected in both farms. Several chemolithotrophic bacteria and archaea were found in the natural reservoir used for aquaculture activities, while water microbiomes in the aquaculture systems were generally dominated by heterotrophic organisms.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Detection of Carbon Steel Corrosion Induced by Fermentative Bacteria From Natural Gas Transmission Lines

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-10 DOI: 10.1111/1758-2229.70058

Joshua A. Davis, Sai Prasanna Chinthala, Chelsea N. Monty-Bromer, John M. Senko

{"title":"Electrochemical Detection of Carbon Steel Corrosion Induced by Fermentative Bacteria From Natural Gas Transmission Lines","authors":"Joshua A. Davis, Sai Prasanna Chinthala, Chelsea N. Monty-Bromer, John M. Senko","doi":"10.1111/1758-2229.70058","DOIUrl":"10.1111/1758-2229.70058","url":null,"abstract":"The metabolic potential and corrosive activities of a fermentative bacterial enrichment culture from a natural gas transmission line were characterised. Three metagenome-assembled genomes (MAGs) attributable to Cytobacillus, Lacrimispora and Staphylococcus spp. were obtained. These MAGs hosted genes involved in the fermentation of carbohydrates to organic acids, which was reflected in the acidification of the growth medium by the culture. To evaluate the corrosive activities of the culture, it was incubated in a split chamber-zero resistance ammetry (SC-ZRA) format. This involved deploying carbon steel coupons immersed in liquid medium in opposing chambers of an electrochemical cell. Measurement of current between the coupons indicated the extent and mechanism of corrosion. When the enrichment culture was added to one side of an SC-ZRA incubation with bicarbonate-buffered medium, pH change and corrosion were minimal. In bicarbonate-free medium, the culture acidified the medium, induced electron transfer from the uninoculated chamber to the inoculated chamber, and caused mass loss. These results indicate that fermenter-induced microbially influenced corrosion (MIC) is due to localised fluid acidification, inducing anodic reactions on the metal surface exposed to the microorganisms and mass loss of the non-exposed metal.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rooting for Success: The Role of Microorganisms in Promoting Growth and Resilience in Black Alder Seedlings

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-06 DOI: 10.1111/1758-2229.70060

Greta Striganavičiūtė, Dorotėja Vaitiekūnaitė, Milana Šilanskienė, Vaida Sirgedaitė-Šėžienė

{"title":"Rooting for Success: The Role of Microorganisms in Promoting Growth and Resilience in Black Alder Seedlings","authors":"Greta Striganavičiūtė, Dorotėja Vaitiekūnaitė, Milana Šilanskienė, Vaida Sirgedaitė-Šėžienė","doi":"10.1111/1758-2229.70060","DOIUrl":"10.1111/1758-2229.70060","url":null,"abstract":"Polycyclic aromatic hydrocarbons (PAHs) pose a global environmental risk, impacting human health. Enhancing phytoremediation with microbial-plant interactions could help mitigate these pollutants. However, tree responses to PAHs are unclear, necessitating controlled studies before field experiments. This study examined how PAH-degrading microbes affect black alder (Alnus glutinosa L.) seedlings grown hydroponically, hypothesizing that specific microbes improve growth and stress tolerance. Two half-sib families (41–65–7 K, 13–99–1 K) were inoculated with Rhodotorula sphaerocarpa (R.s.), Pseudomonas putida (P.p.), and Sphingobium yanoikuyae (S.y.). Results showed family-dependent and microbe-specific effects, with family 41–65–7 K showing enhanced shoot growth (threefold increase by R.s.) and higher carotenoid levels. Antioxidant enzyme activities varied: R.s. elevated superoxide dismutase activity by 4.8-fold in 13–99–1 K, while catalase activity increased but decreased in 41–65–7 K. Principal component analysis revealed distinct phytochemical clustering based on microbial treatment, highlighting genotype-specific modulations. Each microorganism had unique plant growth-promoting traits, with P.p. producing the most phytohormone and S.y. fixing nitrogen. These findings support targeted microbial inoculation for effective remediation of PAH-contaminated environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic acid bacteria: A sustainable solution against phytopathogenic agents

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-02 DOI: 10.1111/1758-2229.70021

Andreia Saragoça, Henrique Canha, Carla M. R. Varanda, Patrick Materatski, Ana Isabel Cordeiro, José Gama

引用次数: 0

Influence of inoculum-to-substrate ratio on biomethane production via anaerobic digestion of biomass

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-02 DOI: 10.1111/1758-2229.70009

Marvin T. Valentin, Daniel Ciolkosz, Andrzej Białowiec

{"title":"Influence of inoculum-to-substrate ratio on biomethane production via anaerobic digestion of biomass","authors":"Marvin T. Valentin, Daniel Ciolkosz, Andrzej Białowiec","doi":"10.1111/1758-2229.70009","DOIUrl":"10.1111/1758-2229.70009","url":null,"abstract":"The influence of the inoculum-to-substrate ratio (ISR) on anaerobic digestion (AD) of biomass in terms of methane yield and microbial community, was explored in this paper. The level of ISR can affect the AD performance in several ways. At extremely low ISR, volatile fatty acids (VFAs) accumuate, while inhibition occur at higher level of ISR. An ISR ranging from 1.0–2.0 was found optimal resulting in higher methane yield, organic matter removal and VFA degradation. Furthermore, a high ISR (2.0–4.0) is favourable to methanogenesis, while a lower ISR (<1.0) is prone to irreversible acidification. The range of ISR can shift the methanogenic pathway of AD to favour an acetoclastic or hydrogenotrophic response, indicated by the enriched group of microorganisms. The genus Methanosaeta (acetoclastic) and Methanobacterium (hydrogenotrophic) are the most enriched methanogens across all ISRs, while Firmicutes, Bacteroidetes, Proteobacteria and Spirochaetae are dominant in the bacterial community. Additionally, the interplay of substrate biodegradability and ISR potentially affects AD performance. Finally, novel equations are developed and proposed for characterizing the quantity of inoculum and substrate.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic acid bacteria: A sustainable solution against phytopathogenic agents

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-02 DOI: 10.1111/1758-2229.70021

Andreia Saragoça, Henrique Canha, Carla M. R. Varanda, Patrick Materatski, Ana Isabel Cordeiro, José Gama

引用次数: 0

Prevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain.

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-12-01 DOI: 10.1111/1758-2229.70055

Jesús Antonio Pérez Jiménez, Silvia Penelo Hidalgo, María-Rosario Baquero Artigao, Gustavo Ortiz-Díez, Tania Ayllón Santiago

{"title":"Prevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain.","authors":"Jesús Antonio Pérez Jiménez, Silvia Penelo Hidalgo, María-Rosario Baquero Artigao, Gustavo Ortiz-Díez, Tania Ayllón Santiago","doi":"10.1111/1758-2229.70055","DOIUrl":"https://doi.org/10.1111/1758-2229.70055","url":null,"abstract":"Managing infections caused by multidrug-resistant Gram-negative bacilli is a major public health concern, particularly in hospitals where surfaces can act as reservoirs for resistant microorganisms. Identifying these bacteria in hospital environments is crucial for improving healthcare safety. This study aimed to analyse environmental samples from a veterinary hospital to identify prevalent microorganisms and detect antimicrobial resistance patterns. A total of 183 surface samples were collected from 26 areas at the Veterinary Clinical Hospital of Alfonso X el Sabio University in Madrid. The isolated strains were identified, and susceptibility profiles were determined via the disk diffusion method. Clonality analysis was performed using pulsed-field gel electrophoresis. In total, 109 strains were isolated: 76.15% from the Enterobacteriaceae family and 23.85% non-fermenting Gram-negative bacilli. The isolates included Klebsiella, Enterobacter, Escherichia and Pseudomonas species, which could include high-risk clones, given their ability to carry several antimicrobial resistance genes. The equine area had the highest number of isolates (n = 71), accounting for 65% of the total. High resistance indices were observed against at least five of the 16 antibiotics tested, indicating significant multidrug resistance. Clonality analysis suggested potential cross-transmission within the facility. This study sampled hospital surfaces but not personnel or animals, making contamination sources unclear. Without resampling, the effectiveness of cleaning protocols remains uncertain. Results suggest that hospital staff play a key role in bacterial transmission. The lack of specialised preventive measures in veterinary hospitals highlights a need for further research and improvement.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":"e70055"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To be or not to be a nonhost species: A case study of the Leptosphaeria maculans and Brassica carinata interaction 成为或不成为非宿主物种：Leptosphaeria maculans 和 Brassica carinata 相互作用的案例研究。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-28 DOI: 10.1111/1758-2229.70034

Julie M. Noah, Mathilde Gorse, Carole-Anne Romain, Elise J. Gay, Thierry Rouxel, Marie-Hélène Balesdent, Jessica L. Soyer

{"title":"To be or not to be a nonhost species: A case study of the Leptosphaeria maculans and Brassica carinata interaction","authors":"Julie M. Noah, Mathilde Gorse, Carole-Anne Romain, Elise J. Gay, Thierry Rouxel, Marie-Hélène Balesdent, Jessica L. Soyer","doi":"10.1111/1758-2229.70034","DOIUrl":"10.1111/1758-2229.70034","url":null,"abstract":"Leptosphaeria maculans is one of the major fungal pathogens on oilseed rape (Brassica napus), causing stem canker disease. The closely related Brassica species B. nigra, B. juncea, and B. carinata display extreme resistance toward stem canker. In this study, we demonstrate the nonhost status of B. carinata toward L. maculans in France through field experiments and inoculations performed in controlled conditions. A few isolates moderately adapted to B. carinata in controlled conditions were recovered in the field on B. nigra leaves, allowing us to investigate the unusual B. carinata–L. maculans interactions using molecular, macroscopic, and microscopic analyses. A cross between a L. maculans isolate adapted to B. napus and an isolate moderately adapted to B. carinata allowed the generation, in the lab, of recombinant L. maculans strains better adapted to B. carinata than the natural parental isolate obtained from B. nigra, and highlighted the polygenic determinism of the adaptation of L. maculans to B. carinata and B. napus. This biological material will allow further investigation of the molecular determinants of the adaptation of L. maculans to nonhost species and elucidate the genetic resistance basis of B. carinata.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory Experiments Suggest a Limited Impact of Increased Nitrogen Deposition on Snow Algae Blooms

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-28 DOI: 10.1111/1758-2229.70052

Pablo Almela, James J. Elser, J. Joseph Giersch, Scott Hotaling, Victoria Rebbeck, Trinity L. Hamilton

{"title":"Laboratory Experiments Suggest a Limited Impact of Increased Nitrogen Deposition on Snow Algae Blooms","authors":"Pablo Almela, James J. Elser, J. Joseph Giersch, Scott Hotaling, Victoria Rebbeck, Trinity L. Hamilton","doi":"10.1111/1758-2229.70052","DOIUrl":"https://doi.org/10.1111/1758-2229.70052","url":null,"abstract":"Snow algal blooms decrease snow albedo and increase local melt rates. However, the causes behind the size and frequency of these blooms are still not well understood. One factor likely contributing is nutrient availability, specifically nitrogen and phosphorus. The nutrient requirements of the taxa responsible for these blooms are not known. Here, we assessed the growth of three commercial strains of snow algae under 24 different nutrient treatments that varied in both absolute and relative concentrations of nitrogen and phosphorus. After 38 days of incubation, we measured total biomass and cell size and estimated their effective albedo reduction surface. Snow algal strains tended to respond similarly and achieved bloom-like cell densities over a wide range of nutrient conditions. However, the molar ratio of nitrogen to phosphorus at which maximum biomass was achieved was between 4 and 7. Our data indicate a high requirement for phosphorus for snow algae and highlights phosphorus availability as a critical factor influencing the frequency and extent of snow algae blooms and their potential contribution to snow melt through altered albedo. Snow algae can thrive across a range of nitrogen (N) and phosphorus (P) conditions, with a higher P requirement for optimal growth. Our study suggests that increased N deposition may have a limited impact on snow algae bloom occurrence and size, emphasising P as a key factor influencing these blooms and their potential to accelerate snow melt by lowering albedo.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory Experiments Suggest a Limited Impact of Increased Nitrogen Deposition on Snow Algae Blooms

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-28 DOI: 10.1111/1758-2229.70052

Pablo Almela, James J. Elser, J. Joseph Giersch, Scott Hotaling, Victoria Rebbeck, Trinity L. Hamilton

{"title":"Laboratory Experiments Suggest a Limited Impact of Increased Nitrogen Deposition on Snow Algae Blooms","authors":"Pablo Almela, James J. Elser, J. Joseph Giersch, Scott Hotaling, Victoria Rebbeck, Trinity L. Hamilton","doi":"10.1111/1758-2229.70052","DOIUrl":"https://doi.org/10.1111/1758-2229.70052","url":null,"abstract":"Snow algal blooms decrease snow albedo and increase local melt rates. However, the causes behind the size and frequency of these blooms are still not well understood. One factor likely contributing is nutrient availability, specifically nitrogen and phosphorus. The nutrient requirements of the taxa responsible for these blooms are not known. Here, we assessed the growth of three commercial strains of snow algae under 24 different nutrient treatments that varied in both absolute and relative concentrations of nitrogen and phosphorus. After 38 days of incubation, we measured total biomass and cell size and estimated their effective albedo reduction surface. Snow algal strains tended to respond similarly and achieved bloom-like cell densities over a wide range of nutrient conditions. However, the molar ratio of nitrogen to phosphorus at which maximum biomass was achieved was between 4 and 7. Our data indicate a high requirement for phosphorus for snow algae and highlights phosphorus availability as a critical factor influencing the frequency and extent of snow algae blooms and their potential contribution to snow melt through altered albedo. Snow algae can thrive across a range of nitrogen (N) and phosphorus (P) conditions, with a higher P requirement for optimal growth. Our study suggests that increased N deposition may have a limited impact on snow algae bloom occurrence and size, emphasising P as a key factor influencing these blooms and their potential to accelerate snow melt by lowering albedo.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0