Environmental Microbiology Reports最新文献

{"title":"The Decrease of Soil Microbial Community Diversity and Network Complexity Results in the Increase of Soil-Borne Diseases With Monocultural Years in Greenhouse Tomato Production Systems","authors":"Jing Hu,&nbsp;Li Wan,&nbsp;Yafang Wang,&nbsp;Kuai Dai,&nbsp;Klaus Butterbach-Bahl,&nbsp;Shan Lin","doi":"10.1111/1758-2229.70165","DOIUrl":"https://doi.org/10.1111/1758-2229.70165","url":null,"abstract":"<p>The excessive use of water and fertiliser, along with long-term monocultivation in greenhouse vegetable fields, has led to a decrease in soil quality and an imbalance in the soil microflora, which may contribute to worsening soil-borne diseases. To examine the associations between the soil microbial community composition and disease-suppressing microorganisms under different years of cultivation shifting from croplands to greenhouses, we collected soil samples from fields continuously planted with tomatoes for 5, 10 and 20 years, as well as from nearby maize–wheat fields (MW). The soil physicochemical properties, microbial community composition, putative plant pathogens and putative antagonistic bacteria were analysed. The results indicated that (1) the diversity and richness of soil bacteria and fungi significantly decreased with longer cultivation years; (2) bacterial and fungal diversity indices were negatively associated with soil nutrient content and positively associated with soil pH and NO₃⁻-N content, the latter being identified as the main factor contributing to the decline in microbial diversity; (3) the complexity of the soil bacterial network initially increased, peaking after 5 years of continuous monoculture, and then decreased, whereas fungal network complexity gradually declined over time; (4) the relative abundance of putative pathogens such as <i>Fusarium</i>, <i>Alternaria</i> and <i>Cladosporium</i> (associated with tomato wilt and leaf mould) increased significantly with longer cultivation, whereas the relative abundance of the bacterial genera associated with putative antagonism <i>Bacillus</i>, <i>Paenibacillus</i> and <i>Streptomyces</i> decreased. In conclusion, after 10 years of continuous monocultivation, a marked reduction in soil microbial diversity and putative antagonistic bacteria was observed, along with an increase in putative pathogenic fungi. These changes likely contributed to the worsening of soil-borne diseases, threatening the sustainability of greenhouse vegetable production.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Decrease of Soil Microbial Community Diversity and Network Complexity Results in the Increase of Soil-Borne Diseases With Monocultural Years in Greenhouse Tomato Production Systems","authors":"Jing Hu,&nbsp;Li Wan,&nbsp;Yafang Wang,&nbsp;Kuai Dai,&nbsp;Klaus Butterbach-Bahl,&nbsp;Shan Lin","doi":"10.1111/1758-2229.70165","DOIUrl":"https://doi.org/10.1111/1758-2229.70165","url":null,"abstract":"<p>The excessive use of water and fertiliser, along with long-term monocultivation in greenhouse vegetable fields, has led to a decrease in soil quality and an imbalance in the soil microflora, which may contribute to worsening soil-borne diseases. To examine the associations between the soil microbial community composition and disease-suppressing microorganisms under different years of cultivation shifting from croplands to greenhouses, we collected soil samples from fields continuously planted with tomatoes for 5, 10 and 20 years, as well as from nearby maize–wheat fields (MW). The soil physicochemical properties, microbial community composition, putative plant pathogens and putative antagonistic bacteria were analysed. The results indicated that (1) the diversity and richness of soil bacteria and fungi significantly decreased with longer cultivation years; (2) bacterial and fungal diversity indices were negatively associated with soil nutrient content and positively associated with soil pH and NO₃⁻-N content, the latter being identified as the main factor contributing to the decline in microbial diversity; (3) the complexity of the soil bacterial network initially increased, peaking after 5 years of continuous monoculture, and then decreased, whereas fungal network complexity gradually declined over time; (4) the relative abundance of putative pathogens such as <i>Fusarium</i>, <i>Alternaria</i> and <i>Cladosporium</i> (associated with tomato wilt and leaf mould) increased significantly with longer cultivation, whereas the relative abundance of the bacterial genera associated with putative antagonism <i>Bacillus</i>, <i>Paenibacillus</i> and <i>Streptomyces</i> decreased. In conclusion, after 10 years of continuous monocultivation, a marked reduction in soil microbial diversity and putative antagonistic bacteria was observed, along with an increase in putative pathogenic fungi. These changes likely contributed to the worsening of soil-borne diseases, threatening the sustainability of greenhouse vegetable production.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Cargo in Membrane Vesicles From a Citrus Pathogen","authors":"Gabriel G. Araujo,&nbsp;Matheus M. Conforte,&nbsp;Aline D. da Purificação,&nbsp;Iris Todeschini,&nbsp;Edgar E. Llontop,&nbsp;Claudia B. Angeli,&nbsp;Alex Inague,&nbsp;Marcos Y. Yoshinaga,&nbsp;Robson F. de Souza,&nbsp;Rodrigo Papai,&nbsp;Maciel S. Luz,&nbsp;Sayuri Miyamoto,&nbsp;Giuseppe Palmisano,&nbsp;Chuck S. Farah,&nbsp;Cristiane R. Guzzo","doi":"10.1111/1758-2229.70101","DOIUrl":"https://doi.org/10.1111/1758-2229.70101","url":null,"abstract":"<p>The causative agent of citrus canker disease, <i>Xanthomonas citri</i> pv. <i>citri</i>, was found to produce copious amounts of outer membrane vesicles (OMVs), frequently forming long membranous tubes under different culture conditions. Lipidomic analysis revealed significant differences in lipid composition between purified vesicles in relation to whole cells. The results suggest an enrichment in saturated cardiolipins and a decrease in unsaturated lipids in the OMV samples. The vesicles' proteome was found to be significantly enriched in TonB-dependent receptors related to the acquisition of different nutrients. These proteins are known to transport siderophores, which were evidenced to be present in purified <i>X. citri</i> OMVs, along with essential metals including iron, zinc, and manganese quantified by elemental analysis. The availability of vesicle-associated nutrients to be incorporated by cells was demonstrated by the use of OMVs as the sole carbon source for bacterial growth. At last, the vesicles also presented esterase and protease activities, which have been associated with virulence in phytopathogens. These evidences point that OMVs from <i>X. citri</i> can share resources within microbial communities, which has potential implications for microbial interactions and plant colonisation, affecting their survival and persistence on the host and in the environment.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Cargo in Membrane Vesicles From a Citrus Pathogen","authors":"Gabriel G. Araujo,&nbsp;Matheus M. Conforte,&nbsp;Aline D. da Purificação,&nbsp;Iris Todeschini,&nbsp;Edgar E. Llontop,&nbsp;Claudia B. Angeli,&nbsp;Alex Inague,&nbsp;Marcos Y. Yoshinaga,&nbsp;Robson F. de Souza,&nbsp;Rodrigo Papai,&nbsp;Maciel S. Luz,&nbsp;Sayuri Miyamoto,&nbsp;Giuseppe Palmisano,&nbsp;Chuck S. Farah,&nbsp;Cristiane R. Guzzo","doi":"10.1111/1758-2229.70101","DOIUrl":"https://doi.org/10.1111/1758-2229.70101","url":null,"abstract":"<p>The causative agent of citrus canker disease, <i>Xanthomonas citri</i> pv. <i>citri</i>, was found to produce copious amounts of outer membrane vesicles (OMVs), frequently forming long membranous tubes under different culture conditions. Lipidomic analysis revealed significant differences in lipid composition between purified vesicles in relation to whole cells. The results suggest an enrichment in saturated cardiolipins and a decrease in unsaturated lipids in the OMV samples. The vesicles' proteome was found to be significantly enriched in TonB-dependent receptors related to the acquisition of different nutrients. These proteins are known to transport siderophores, which were evidenced to be present in purified <i>X. citri</i> OMVs, along with essential metals including iron, zinc, and manganese quantified by elemental analysis. The availability of vesicle-associated nutrients to be incorporated by cells was demonstrated by the use of OMVs as the sole carbon source for bacterial growth. At last, the vesicles also presented esterase and protease activities, which have been associated with virulence in phytopathogens. These evidences point that OMVs from <i>X. citri</i> can share resources within microbial communities, which has potential implications for microbial interactions and plant colonisation, affecting their survival and persistence on the host and in the environment.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microdiversity Shapes the Seasonal Niche of Prokaryotic Plankton Inhabiting Surface Waters in a Coastal Upwelling System","authors":"Cessna-Pamela Orta-Ponce,&nbsp;Rodrigo Alba-Salgueiro,&nbsp;Carlota Rodríguez,&nbsp;Joaquín Valencia-Vila,&nbsp;Pilar Díaz-Tapia,&nbsp;Antonio Bode,&nbsp;Mar Nieto-Cid,&nbsp;Marta M. Varela","doi":"10.1111/1758-2229.70131","DOIUrl":"https://doi.org/10.1111/1758-2229.70131","url":null,"abstract":"<p>Seasonality of prokaryotic abundance, diversity and community composition was investigated over a 2-year period in a coastal upwelling time-series station. A marked seasonality was found for prokaryotic abundance, peaking during upwelling and upwelling-to-downwelling transition, and decreasing during downwelling. The latter included a deeper mixed layer and a homogeneous water column favouring higher abundance of archaea (i.e., Marine Group II, <i>Candiadatus nitrosopelagicus</i>), SAR406 clade and the group Bacteria_Others including &gt; 400 rare taxa. Upwelling and transition conditions, characterised by enhanced vertical stratification and a marked hydrographic variability, included a community less diverse with core-phylotypes proliferating, i.e., Flavobacteriaceae, Amylibacter and Planktomarina. Physical and biogeochemical variables collectively explained &gt; 40% of the seasonal changes in prokaryotic assemblages. Additionally, fine-tune bacterial features evidenced ‘closely related taxa’ within particular bacterial phylotypes such as SAR116 clade; certain Flavobacteria belonging to NS2b, NS4 or NS9; members of the family Cryomorphaceae and Marine Group II, displaying seasonal microdiversity patterns. Taken together, seasonal hydrographic forcing induces a shift in the upwelling-driven microbiome providing new insights into the barely explored seasonal niche partitioning of surface prokaryotic communities in such highly productive upwelling systems. These results are of broad interest for understanding ecosystem functioning and forecast the impacts of current environmental change.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microdiversity Shapes the Seasonal Niche of Prokaryotic Plankton Inhabiting Surface Waters in a Coastal Upwelling System","authors":"Cessna-Pamela Orta-Ponce,&nbsp;Rodrigo Alba-Salgueiro,&nbsp;Carlota Rodríguez,&nbsp;Joaquín Valencia-Vila,&nbsp;Pilar Díaz-Tapia,&nbsp;Antonio Bode,&nbsp;Mar Nieto-Cid,&nbsp;Marta M. Varela","doi":"10.1111/1758-2229.70131","DOIUrl":"https://doi.org/10.1111/1758-2229.70131","url":null,"abstract":"<p>Seasonality of prokaryotic abundance, diversity and community composition was investigated over a 2-year period in a coastal upwelling time-series station. A marked seasonality was found for prokaryotic abundance, peaking during upwelling and upwelling-to-downwelling transition, and decreasing during downwelling. The latter included a deeper mixed layer and a homogeneous water column favouring higher abundance of archaea (i.e., Marine Group II, <i>Candiadatus nitrosopelagicus</i>), SAR406 clade and the group Bacteria_Others including &gt; 400 rare taxa. Upwelling and transition conditions, characterised by enhanced vertical stratification and a marked hydrographic variability, included a community less diverse with core-phylotypes proliferating, i.e., Flavobacteriaceae, Amylibacter and Planktomarina. Physical and biogeochemical variables collectively explained &gt; 40% of the seasonal changes in prokaryotic assemblages. Additionally, fine-tune bacterial features evidenced ‘closely related taxa’ within particular bacterial phylotypes such as SAR116 clade; certain Flavobacteria belonging to NS2b, NS4 or NS9; members of the family Cryomorphaceae and Marine Group II, displaying seasonal microdiversity patterns. Taken together, seasonal hydrographic forcing induces a shift in the upwelling-driven microbiome providing new insights into the barely explored seasonal niche partitioning of surface prokaryotic communities in such highly productive upwelling systems. These results are of broad interest for understanding ecosystem functioning and forecast the impacts of current environmental change.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Difficulty to Detect Carbapenem Resistance in the Environment: Characterisation of Escherichia coli With Reduced Carbapenem Susceptibility Isolated in a French River","authors":"Le Devendec Laëtitia,&nbsp;Tran Mai-Lan,&nbsp;Jouy Eric,&nbsp;Vorimore Fabien,&nbsp;Wilhelm Amandine,&nbsp;Gassilloud Benoit,&nbsp;Fach Patrick,&nbsp;Baron Sandrine,&nbsp;Delannoy Sabine","doi":"10.1111/1758-2229.70162","DOIUrl":"https://doi.org/10.1111/1758-2229.70162","url":null,"abstract":"<p>During an 18-month longitudinal study, bi-monthly water samples were taken upstream and downstream of a watershed. In order to detect carbapenem-resistant <i>E. coli</i>, the CHROMIDCarba medium was used. Of the 863 isolates collected from 144 samples, <i>E. coli</i> identification was confirmed for only seven of them, isolated on the same day. For six isolates, a slightly reduced susceptibility to carbapenems was observed. The results of the whole genome sequencing indicate that the six isolates belong to the same clone (O8:H7, ST196). Furthermore, a mutation of the <i>ompC</i> porin coupled with the presence of the <i>bla</i>_CMY-2 gene, on an <i>IncI1</i> plasmid, would be at the origin of the reduced sensitivity of these strains to carbapenems. This type of mechanism has already been described in human clinical cases. To our knowledge, this is the first time that it has been identified in strains from the aquatic environment. The detection of <i>E. coli</i> with reduced susceptibility to carbapenems one time in 18 months (one out of 36 sampling dates) could be considered a one-time event. However, this illustrates the importance of monitoring the aquatic environment but also the methodological difficulties of such surveillance due to the poor efficacy of the isolation method.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Difficulty to Detect Carbapenem Resistance in the Environment: Characterisation of Escherichia coli With Reduced Carbapenem Susceptibility Isolated in a French River","authors":"Le Devendec Laëtitia,&nbsp;Tran Mai-Lan,&nbsp;Jouy Eric,&nbsp;Vorimore Fabien,&nbsp;Wilhelm Amandine,&nbsp;Gassilloud Benoit,&nbsp;Fach Patrick,&nbsp;Baron Sandrine,&nbsp;Delannoy Sabine","doi":"10.1111/1758-2229.70162","DOIUrl":"https://doi.org/10.1111/1758-2229.70162","url":null,"abstract":"<p>During an 18-month longitudinal study, bi-monthly water samples were taken upstream and downstream of a watershed. In order to detect carbapenem-resistant <i>E. coli</i>, the CHROMIDCarba medium was used. Of the 863 isolates collected from 144 samples, <i>E. coli</i> identification was confirmed for only seven of them, isolated on the same day. For six isolates, a slightly reduced susceptibility to carbapenems was observed. The results of the whole genome sequencing indicate that the six isolates belong to the same clone (O8:H7, ST196). Furthermore, a mutation of the <i>ompC</i> porin coupled with the presence of the <i>bla</i>_CMY-2 gene, on an <i>IncI1</i> plasmid, would be at the origin of the reduced sensitivity of these strains to carbapenems. This type of mechanism has already been described in human clinical cases. To our knowledge, this is the first time that it has been identified in strains from the aquatic environment. The detection of <i>E. coli</i> with reduced susceptibility to carbapenems one time in 18 months (one out of 36 sampling dates) could be considered a one-time event. However, this illustrates the importance of monitoring the aquatic environment but also the methodological difficulties of such surveillance due to the poor efficacy of the isolation method.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Responses of Oceanic and Coastal Synechococcus to Iron Limitation and Warming Interactions","authors":"Ran Duan,&nbsp;Min Xu,&nbsp;Xiaopeng Bian,&nbsp;Conner Y. Kojima,&nbsp;Shengwei Hou,&nbsp;Qiang Zheng,&nbsp;Seth G. John,&nbsp;David A. Hutchins,&nbsp;Fei-Xue Fu","doi":"10.1111/1758-2229.70158","DOIUrl":"https://doi.org/10.1111/1758-2229.70158","url":null,"abstract":"<p>This study explored the contrasting physiological and transcriptional responses to iron (Fe) and warming temperature interactions in two South China Sea <i>Synechococcus</i> isolates belonging to clade II from the open ocean and CB5 from the coastal ocean. The two picocyanobacterial strains utilised contrasting photosynthesis, Fe uptake, and nutrient acquisition strategies to cope with Fe limitation. In the oceanic strain, moderate warming under Fe limitation upregulated expression of photosynthesis and nutrient and Fe transport genes, increasing its growth and photosynthesis. In contrast, gene expression under low Fe in the coastal strain was less affected by warming. The oceanic isolate exhibited substrate regulation of Fe acquisition and preferred organic nutrient sources. The coastal strain had a much higher Fe quota, faster turnover of the D1 gene in photosystem II, and was optimised for inorganic nitrogen sources. Both strains showed multi-tiered Fe uptake strategies and general stress responses to heat shock and oxidative stress. In general, gene regulation in the oceanic strain responded more effectively to both stressors than in the coastal isolate. Fe-temperature interactions in both strains are complex and may lead to synergistic and antagonistic responses, potentially influencing global biogeochemical cycles in warmer oceans.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Responses of Oceanic and Coastal Synechococcus to Iron Limitation and Warming Interactions","authors":"Ran Duan,&nbsp;Min Xu,&nbsp;Xiaopeng Bian,&nbsp;Conner Y. Kojima,&nbsp;Shengwei Hou,&nbsp;Qiang Zheng,&nbsp;Seth G. John,&nbsp;David A. Hutchins,&nbsp;Fei-Xue Fu","doi":"10.1111/1758-2229.70158","DOIUrl":"https://doi.org/10.1111/1758-2229.70158","url":null,"abstract":"<p>This study explored the contrasting physiological and transcriptional responses to iron (Fe) and warming temperature interactions in two South China Sea <i>Synechococcus</i> isolates belonging to clade II from the open ocean and CB5 from the coastal ocean. The two picocyanobacterial strains utilised contrasting photosynthesis, Fe uptake, and nutrient acquisition strategies to cope with Fe limitation. In the oceanic strain, moderate warming under Fe limitation upregulated expression of photosynthesis and nutrient and Fe transport genes, increasing its growth and photosynthesis. In contrast, gene expression under low Fe in the coastal strain was less affected by warming. The oceanic isolate exhibited substrate regulation of Fe acquisition and preferred organic nutrient sources. The coastal strain had a much higher Fe quota, faster turnover of the D1 gene in photosystem II, and was optimised for inorganic nitrogen sources. Both strains showed multi-tiered Fe uptake strategies and general stress responses to heat shock and oxidative stress. In general, gene regulation in the oceanic strain responded more effectively to both stressors than in the coastal isolate. Fe-temperature interactions in both strains are complex and may lead to synergistic and antagonistic responses, potentially influencing global biogeochemical cycles in warmer oceans.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}