Environmental Microbiology Reports最新文献

Biotechnological Potential of a Novel Strain of Fusarium proliferatum, a Terrestrial Fungus Adapted to Marine Environment 一株适应海洋环境的陆生真菌增殖镰刀菌的生物技术潜力

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-04 DOI: 10.1111/1758-2229.70143

Antonio Nappo, Michela Salamone, Marco Masi, Michela Morelli, Martina Annunziata, Michele Sonnessa, Alessio Cimmino, Andrea Bosso, Rosanna Culurciello, Ilaria Di Nardo, Elio Pizzo, Maria Costantini, Valerio Zupo, Francesco Aliberti, Marco Guida, Federica Carraturo

{"title":"Biotechnological Potential of a Novel Strain of Fusarium proliferatum, a Terrestrial Fungus Adapted to Marine Environment","authors":"Antonio Nappo, Michela Salamone, Marco Masi, Michela Morelli, Martina Annunziata, Michele Sonnessa, Alessio Cimmino, Andrea Bosso, Rosanna Culurciello, Ilaria Di Nardo, Elio Pizzo, Maria Costantini, Valerio Zupo, Francesco Aliberti, Marco Guida, Federica Carraturo","doi":"10.1111/1758-2229.70143","DOIUrl":"https://doi.org/10.1111/1758-2229.70143","url":null,"abstract":"Marine habitats represent hostile environments for the majority of microorganisms. Nonetheless, in the last decades, the study of the microbial diversity of the halophylic environments has reported that fungi constitute a quantitatively relevant component. The research reports the isolation of a novel strain of Fusarium proliferatum from seawater, within a monitoring campaign conducted in the South Calabrian coasts (Regione Calabria, Italy): the microorganism presumably adapted from a terrestrial to a marine niche, potentially changing its metabolism in response to the environmental stress. The marine fungus was molecularly characterised preliminarily with Sanger Sequencing, and further employing Whole Genome Sequencing, subsequently cultivated on organic rice to stimulate the production of secondary metabolites. Chemical extraction and purification processes yielded three main compounds identified as 9-O-methylbostrycoidin (MBC), 9-O-methylfusarubin (MFR) and 3-indoleacetic acid (IAA). When tested for their antimicrobial and antioxidant potential, MBC and MFR demonstrated significant activity against Staphylococcus aureus and Listeria monocytogenes, while IAA exhibited no antimicrobial effect, but highlighted antioxidant properties with the ORAC assay. Additionally, biocompatibility assays on human keratinocyte cells (HaCaT) revealed minimal toxicity of the crude extract and MBC, while IAA displayed dose-dependent toxicity, opening to considering the purified secondary metabolites for valuable applications in environmental, industrial and pharmaceutical biotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological Potential of a Novel Strain of Fusarium proliferatum, a Terrestrial Fungus Adapted to Marine Environment 一株适应海洋环境的陆生真菌增殖镰刀菌的生物技术潜力

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-04 DOI: 10.1111/1758-2229.70143

Antonio Nappo, Michela Salamone, Marco Masi, Michela Morelli, Martina Annunziata, Michele Sonnessa, Alessio Cimmino, Andrea Bosso, Rosanna Culurciello, Ilaria Di Nardo, Elio Pizzo, Maria Costantini, Valerio Zupo, Francesco Aliberti, Marco Guida, Federica Carraturo

{"title":"Biotechnological Potential of a Novel Strain of Fusarium proliferatum, a Terrestrial Fungus Adapted to Marine Environment","authors":"Antonio Nappo, Michela Salamone, Marco Masi, Michela Morelli, Martina Annunziata, Michele Sonnessa, Alessio Cimmino, Andrea Bosso, Rosanna Culurciello, Ilaria Di Nardo, Elio Pizzo, Maria Costantini, Valerio Zupo, Francesco Aliberti, Marco Guida, Federica Carraturo","doi":"10.1111/1758-2229.70143","DOIUrl":"https://doi.org/10.1111/1758-2229.70143","url":null,"abstract":"Marine habitats represent hostile environments for the majority of microorganisms. Nonetheless, in the last decades, the study of the microbial diversity of the halophylic environments has reported that fungi constitute a quantitatively relevant component. The research reports the isolation of a novel strain of Fusarium proliferatum from seawater, within a monitoring campaign conducted in the South Calabrian coasts (Regione Calabria, Italy): the microorganism presumably adapted from a terrestrial to a marine niche, potentially changing its metabolism in response to the environmental stress. The marine fungus was molecularly characterised preliminarily with Sanger Sequencing, and further employing Whole Genome Sequencing, subsequently cultivated on organic rice to stimulate the production of secondary metabolites. Chemical extraction and purification processes yielded three main compounds identified as 9-O-methylbostrycoidin (MBC), 9-O-methylfusarubin (MFR) and 3-indoleacetic acid (IAA). When tested for their antimicrobial and antioxidant potential, MBC and MFR demonstrated significant activity against Staphylococcus aureus and Listeria monocytogenes, while IAA exhibited no antimicrobial effect, but highlighted antioxidant properties with the ORAC assay. Additionally, biocompatibility assays on human keratinocyte cells (HaCaT) revealed minimal toxicity of the crude extract and MBC, while IAA displayed dose-dependent toxicity, opening to considering the purified secondary metabolites for valuable applications in environmental, industrial and pharmaceutical biotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Metabarcoding Focusing on Eukaryote Communities on Langhovde Glacier, East Antarctica 南极东部Langhovde冰川真核生物群落的DNA元条形码研究

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-02 DOI: 10.1111/1758-2229.70117

Hiroto Kajita, Ken Kondo, Shin Sugiyama, Yasuhide Nakamura, Sakae Kudoh, Koji Umeda

{"title":"DNA Metabarcoding Focusing on Eukaryote Communities on Langhovde Glacier, East Antarctica","authors":"Hiroto Kajita, Ken Kondo, Shin Sugiyama, Yasuhide Nakamura, Sakae Kudoh, Koji Umeda","doi":"10.1111/1758-2229.70117","DOIUrl":"https://doi.org/10.1111/1758-2229.70117","url":null,"abstract":"Ecological structures and habitats of eukaryote communities in supraglacial environments have attracted attention because of their unique biodiversity and potential impact on glacial surface melt. In this study, we investigated the microbial community on Langhovde Glacier in East Antarctica, wherein few molecular biological studies have been conducted. We performed a comprehensive environmental DNA analysis and dissolved ion measurements focusing on various types of supraglacial waters scattered on Langhovde Glacier, as well as ponds in the adjacent off-ice area. 18S rRNA gene analysis revealed the presence of diverse eukaryotic taxa on the glacier, including Chlorophyta, Chrysophyceae, Cercozoa, Choanoflagellatea and Streptophyta. Distinct ecological structures were observed between large perennial supraglacial lakes and small transient supraglacial puddles on the glacier. Moreover, microbial diversity was greater in the off-ice ponds with elevated concentrations of dissolved ions. Only a limited number of eukaryotic gene sequences were shared on- and off-ice sites, and many of the gene sequences detected on Langhovde Glacier matched those from remote snow and ice fields worldwide. These results highlight the cosmopolitan nature of ice/snow algae and suggest that the physicochemical properties of the supraglacial water environment play a crucial role in shaping microbial diversity on glacier surfaces.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogeographic Patterns and Ecological Roles of Microorganisms in Sediments Along an Estuarine Salinity Gradient 河口盐度梯度沉积物中微生物的生物地理格局和生态作用

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-02 DOI: 10.1111/1758-2229.70139

Zongxiao Zhang, Guo Yuan, Xakila Turgun, Zulpinur Turgun, Lijun Hou, Mao Ye, Yonghui Wang, Xingbin Xu

{"title":"Biogeographic Patterns and Ecological Roles of Microorganisms in Sediments Along an Estuarine Salinity Gradient","authors":"Zongxiao Zhang, Guo Yuan, Xakila Turgun, Zulpinur Turgun, Lijun Hou, Mao Ye, Yonghui Wang, Xingbin Xu","doi":"10.1111/1758-2229.70139","DOIUrl":"https://doi.org/10.1111/1758-2229.70139","url":null,"abstract":"The distribution patterns and driving mechanisms of microbial biogeographic patterns are fundamental questions in microbiology. This study analysed and compared the bacterial biogeographic patterns in the coastal environment, focusing on the Yangtze Estuary and its adjacent coastal zone. The purpose is to explore the driving mechanisms under spatial distribution, the community assembly processes and potential functions. Our results revealed that the sediment bacterial community structure exhibited a distinct geographical pattern and was significantly influenced by environmental factors. The microbial community displayed a non-random co-occurrence pattern, and the biogeographic patterns were shaped not only by environmental constraints (deterministic processes) but also by stochastic processes resulting from dispersal limitation. The metagenome sequencing analysis revealed a pronounced salinity gradient in the nitrogen-cycling function of the bacterial community. This functional difference appears to be driven by microbial diversity changes from the estuarine region to the ocean, highlighting the key role of microbial ecological characteristics. The findings of this study contribute to a deeper understanding of microbial ecology in estuarine environments, emphasizing the complex interplay between environmental factors and microbial community dynamics in shaping the function of estuarine sediment bacterial communities.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Metabarcoding Focusing on Eukaryote Communities on Langhovde Glacier, East Antarctica 南极东部Langhovde冰川真核生物群落的DNA元条形码研究

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-02 DOI: 10.1111/1758-2229.70117

Hiroto Kajita, Ken Kondo, Shin Sugiyama, Yasuhide Nakamura, Sakae Kudoh, Koji Umeda

{"title":"DNA Metabarcoding Focusing on Eukaryote Communities on Langhovde Glacier, East Antarctica","authors":"Hiroto Kajita, Ken Kondo, Shin Sugiyama, Yasuhide Nakamura, Sakae Kudoh, Koji Umeda","doi":"10.1111/1758-2229.70117","DOIUrl":"https://doi.org/10.1111/1758-2229.70117","url":null,"abstract":"Ecological structures and habitats of eukaryote communities in supraglacial environments have attracted attention because of their unique biodiversity and potential impact on glacial surface melt. In this study, we investigated the microbial community on Langhovde Glacier in East Antarctica, wherein few molecular biological studies have been conducted. We performed a comprehensive environmental DNA analysis and dissolved ion measurements focusing on various types of supraglacial waters scattered on Langhovde Glacier, as well as ponds in the adjacent off-ice area. 18S rRNA gene analysis revealed the presence of diverse eukaryotic taxa on the glacier, including Chlorophyta, Chrysophyceae, Cercozoa, Choanoflagellatea and Streptophyta. Distinct ecological structures were observed between large perennial supraglacial lakes and small transient supraglacial puddles on the glacier. Moreover, microbial diversity was greater in the off-ice ponds with elevated concentrations of dissolved ions. Only a limited number of eukaryotic gene sequences were shared on- and off-ice sites, and many of the gene sequences detected on Langhovde Glacier matched those from remote snow and ice fields worldwide. These results highlight the cosmopolitan nature of ice/snow algae and suggest that the physicochemical properties of the supraglacial water environment play a crucial role in shaping microbial diversity on glacier surfaces.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogeographic Patterns and Ecological Roles of Microorganisms in Sediments Along an Estuarine Salinity Gradient 河口盐度梯度沉积物中微生物的生物地理格局和生态作用

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-02 DOI: 10.1111/1758-2229.70139

Zongxiao Zhang, Guo Yuan, Xakila Turgun, Zulpinur Turgun, Lijun Hou, Mao Ye, Yonghui Wang, Xingbin Xu

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引用次数: 0

Optimisation of Laccase Activity From Bacillus atrophaeus Using Response Surface Methodology: A Proof-Of-Concept Dye Decolourisation Study 利用响应面法优化萎缩芽孢杆菌漆酶活性：一项概念验证染料脱色研究

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-01 DOI: 10.1111/1758-2229.70138

Kubra Kocak, Arzu Altin Yavuz, Suleyman Berberler, Cansu Filik Iscen

{"title":"Optimisation of Laccase Activity From Bacillus atrophaeus Using Response Surface Methodology: A Proof-Of-Concept Dye Decolourisation Study","authors":"Kubra Kocak, Arzu Altin Yavuz, Suleyman Berberler, Cansu Filik Iscen","doi":"10.1111/1758-2229.70138","DOIUrl":"https://doi.org/10.1111/1758-2229.70138","url":null,"abstract":"This study investigates the enhancement of laccase activity, a copper-containing enzyme involved in oxidative biodegradation. The enzyme was studied in Bacillus atrophaeus, newly isolated from paper mill wastewater. Initial optimisation of key factors, including carbon and nitrogen sources, incubation time, inoculum size, pH, temperature, and CuSO4 concentration. Subsequently, a systematic refinement of selected parameters was performed through response surface methodology, a statistical optimisation technique. The maximum laccase activity of 0.057 U/mL was achieved under the following conditions: pH 8.0, 35.28°C, 1.5% CuSO4, 0.5% inoculum size, 3.7 g/L fructose and 1.08 g/L yeast extract. Under these conditions, a 2.51-fold enhancement in enzymatic activity was achieved compared to pre-optimised conditions. The optimised enzyme activity was then tested for its ability to decolourise dyes, specifically Congo red, burazol black and burazol navy. Congo red decolourisation exhibited a 2.95-fold increase after 72 h under optimised conditions, whereas burazol black and burazol navy dyes remained unaffected. These findings underscore the potential of optimised laccase-based methods for efficient dye wastewater treatment. Using response surface methodology, key parameters were fine-tuned to enhance laccase activity and decolourisation efficiency, advancing sustainable bioremediation in environmental biotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimisation of Laccase Activity From Bacillus atrophaeus Using Response Surface Methodology: A Proof-Of-Concept Dye Decolourisation Study 利用响应面法优化萎缩芽孢杆菌漆酶活性：一项概念验证染料脱色研究

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-07-01 DOI: 10.1111/1758-2229.70138

Kubra Kocak, Arzu Altin Yavuz, Suleyman Berberler, Cansu Filik Iscen

{"title":"Optimisation of Laccase Activity From Bacillus atrophaeus Using Response Surface Methodology: A Proof-Of-Concept Dye Decolourisation Study","authors":"Kubra Kocak, Arzu Altin Yavuz, Suleyman Berberler, Cansu Filik Iscen","doi":"10.1111/1758-2229.70138","DOIUrl":"https://doi.org/10.1111/1758-2229.70138","url":null,"abstract":"This study investigates the enhancement of laccase activity, a copper-containing enzyme involved in oxidative biodegradation. The enzyme was studied in Bacillus atrophaeus, newly isolated from paper mill wastewater. Initial optimisation of key factors, including carbon and nitrogen sources, incubation time, inoculum size, pH, temperature, and CuSO4 concentration. Subsequently, a systematic refinement of selected parameters was performed through response surface methodology, a statistical optimisation technique. The maximum laccase activity of 0.057 U/mL was achieved under the following conditions: pH 8.0, 35.28°C, 1.5% CuSO4, 0.5% inoculum size, 3.7 g/L fructose and 1.08 g/L yeast extract. Under these conditions, a 2.51-fold enhancement in enzymatic activity was achieved compared to pre-optimised conditions. The optimised enzyme activity was then tested for its ability to decolourise dyes, specifically Congo red, burazol black and burazol navy. Congo red decolourisation exhibited a 2.95-fold increase after 72 h under optimised conditions, whereas burazol black and burazol navy dyes remained unaffected. These findings underscore the potential of optimised laccase-based methods for efficient dye wastewater treatment. Using response surface methodology, key parameters were fine-tuned to enhance laccase activity and decolourisation efficiency, advancing sustainable bioremediation in environmental biotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System 工业水冷系统中军团菌的季节性宿主变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-30 DOI: 10.1111/1758-2229.70132

Suzanne Crull, Emlyn Hammer, Allison E. Mann, Lauren M. O'Connell, Ashlyn Soule, Elizabeth Griffith, Thomas Blouin, Robin L. Brigmon, Vincent P. Richards

{"title":"Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System","authors":"Suzanne Crull, Emlyn Hammer, Allison E. Mann, Lauren M. O'Connell, Ashlyn Soule, Elizabeth Griffith, Thomas Blouin, Robin L. Brigmon, Vincent P. Richards","doi":"10.1111/1758-2229.70132","DOIUrl":"https://doi.org/10.1111/1758-2229.70132","url":null,"abstract":"Legionella is a genus of environmental bacteria containing pathogenic species such as Legionella pneumophila that are responsible for Legionnaires' disease, a potentially fatal respiratory infection. Disease aetiology can involve Legionella replication intracellularly within protists and this study aimed to characterise the Legionella-protist relationship to develop novel outbreak prevention targets. Water and sediment samples were collected from a water-cooling tower in South Carolina over a 6-month period. Concomitantly, multiple environmental parameters were recorded. Bacterial and eukaryotic communities were characterised using 16S rRNA gene V4 region and a 252 bp fragment of 18S rRNA gene, respectively. Co-occurrence network analyses were performed to elucidate Legionella-protist correlations through time. We found that Legionella correlated with different protists as the seasons progressed. Acanthamoeba correlated with Legionella in early spring followed by Vannella and Korotnevella in late spring and early summer, and were joined by Echinamoeba in mid-summer. Vannella and Acanthamoeba are known potential hosts for Legionella, while Korotnevella is a potential undocumented host. Of the environmental parameters, temperature showed strong correlation with protists genera, suggesting that Legionella abundance was driven by temperature-dependent protist availability. Our results highlight ecological shifts that are associated with elevated Legionella levels, which offers potential targets to help predict and prevent disease outbreaks.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System 工业水冷系统中军团菌的季节性宿主变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-30 DOI: 10.1111/1758-2229.70132

Suzanne Crull, Emlyn Hammer, Allison E. Mann, Lauren M. O'Connell, Ashlyn Soule, Elizabeth Griffith, Thomas Blouin, Robin L. Brigmon, Vincent P. Richards

{"title":"Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System","authors":"Suzanne Crull, Emlyn Hammer, Allison E. Mann, Lauren M. O'Connell, Ashlyn Soule, Elizabeth Griffith, Thomas Blouin, Robin L. Brigmon, Vincent P. Richards","doi":"10.1111/1758-2229.70132","DOIUrl":"https://doi.org/10.1111/1758-2229.70132","url":null,"abstract":"Legionella is a genus of environmental bacteria containing pathogenic species such as Legionella pneumophila that are responsible for Legionnaires' disease, a potentially fatal respiratory infection. Disease aetiology can involve Legionella replication intracellularly within protists and this study aimed to characterise the Legionella-protist relationship to develop novel outbreak prevention targets. Water and sediment samples were collected from a water-cooling tower in South Carolina over a 6-month period. Concomitantly, multiple environmental parameters were recorded. Bacterial and eukaryotic communities were characterised using 16S rRNA gene V4 region and a 252 bp fragment of 18S rRNA gene, respectively. Co-occurrence network analyses were performed to elucidate Legionella-protist correlations through time. We found that Legionella correlated with different protists as the seasons progressed. Acanthamoeba correlated with Legionella in early spring followed by Vannella and Korotnevella in late spring and early summer, and were joined by Echinamoeba in mid-summer. Vannella and Acanthamoeba are known potential hosts for Legionella, while Korotnevella is a potential undocumented host. Of the environmental parameters, temperature showed strong correlation with protists genera, suggesting that Legionella abundance was driven by temperature-dependent protist availability. Our results highlight ecological shifts that are associated with elevated Legionella levels, which offers potential targets to help predict and prevent disease outbreaks.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0