Environmental Microbiology Reports最新文献

{"title":"Genomic Analysis and Virulence Features of Vibrio cholerae Non-O1/Non-O139 Harbouring CARB-Type β-Lactamases From Freshwater Bodies, Argentina","authors":"Daiana Guevara Núñez,&nbsp;Fabrizzio N. Morandini,&nbsp;Geehan Suleyman,&nbsp;Kyle Crooker,&nbsp;Jagjeet Kaur,&nbsp;Gina Maki,&nbsp;José L. Bocco,&nbsp;Darío Fernández Do Porto,&nbsp;Markus J. Zervos,&nbsp;Claudia Sola,&nbsp;H. Alex Saka","doi":"10.1111/1758-2229.70181","DOIUrl":"https://doi.org/10.1111/1758-2229.70181","url":null,"abstract":"<p><i>Vibrio cholerae</i> is a globally distributed, free-living bacterium in aquatic ecosystems. While non-O1/non-O139 serogroups typically do not produce cholera toxin, they have the potential to cause diarrhoea. These strains may act as reservoirs of antibiotic resistance in rivers, lakes and oceans. Understanding their genetic resistance and virulence can shed light on their role in spreading antimicrobial resistance and their pathogenicity. In this study, we characterised 60 <i>V. cholerae</i> non-O1/non-O139 strains from 16 freshwater bodies located throughout the Province of Córdoba, Argentina. We found none of the strains carried cholera toxin and identified ampicillin resistance as the most prevalent phenotype. Whole genome sequencing revealed that all ampicillin-resistant strains (<i>n</i> = 10) carried CARB β-lactamases, leading to the identification of new CARB variants (CARB-59 to CARB-62) likely associated with the <i>V. cholerae</i> superintegron. Two strains were notably related and exhibited enhanced virulence due to an unusual genetic arrangement of the VPI-1 pathogenicity island, encoding both the toxin co-regulated pilus and a type VI secretion system cluster subclass i5, commonly found in non-cholera <i>Vibrio</i> species. These findings provide significant insights into the genetic diversity and virulent potential of ampicillin-resistant environmental <i>V. cholerae</i> non-O1/non-O139 and enhance our understanding of the evolution of CARB β-lactamases within the species.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inducible Volatile Chemical Signalling Drives Antifungal Activity of Trichoderma hamatum GD12 During Confrontation With the Pathogen Sclerotinia sclerotiorum","authors":"Gareth A. Thomas,&nbsp;József Vuts,&nbsp;David M. Withall,&nbsp;John C. Caulfield,&nbsp;John Sidda,&nbsp;Murray R. Grant,&nbsp;Christopher R. Thornton,&nbsp;Michael A. Birkett","doi":"10.1111/1758-2229.70192","DOIUrl":"10.1111/1758-2229.70192","url":null,"abstract":"<p>The use of beneficial soil fungi or their natural products offers a more sustainable alternative to synthetic fungicides for pathogen management in crops. Volatile organic compounds (VOCs) produced by such fungi act as semiochemicals that inhibit pathogens, with VOC production influenced by physical interactions between competing fungi. This study explores the interaction between the beneficial soil fungus <i>Trichoderma hamatum</i> GD12 strain (GD12), previously shown to antagonise crop pathogens such as <i>Sclerotinia sclerotiorum</i>, to test the hypothesis that its antagonistic effect is mediated by volatile chemical signalling. In dual-culture confrontation assays, co-inoculation of GD12 and <i>S. sclerotiorum</i> led to fungistatic interactions after 7 days. VOCs collected from individual and co-cultures were analysed by gas chromatography–flame ionisation detector (GC-FID) analysis and coupled GC-mass spectrometry (GC–MS), revealing significant differences in VOC production between treatments, with VOC production notably upregulated in the GD12 + <i>S. sclerotiorum</i> co-culture. Peak VOC production occurred 17 days post-inoculation. Synthetic VOC assays revealed several compounds inhibitory to <i>S. sclerotiorum</i>, including 1-octen-3-one, which also arrested the growth of other fungal crop pathogens (<i>Botrytis cinerea</i>, <i>Pyrenopeziza brassicae</i>, and <i>Gaeumannomyces tritici</i>). Structural insights into 1-octen-3-one's antifungal activity against <i>S. sclerotiorum</i> are also presented. These findings support the hypothesis that the antagonistic properties of <i>T. hamatum</i> GD12 against crop fungal pathogens can, in part, be attributed to VOC production. Further research is needed to assess the potential of these semiochemicals as tools for pathogen management in agriculture.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and Spatial Survey on the Abundance of Amoebae and Bacteria in an Estuary and the Role of Environmental Parameters","authors":"Gaëlle Bednarek,&nbsp;Hélène Agogué,&nbsp;Arno Bringer,&nbsp;Tony Agion,&nbsp;Vincent Delafont,&nbsp;Yann Héchard","doi":"10.1111/1758-2229.70198","DOIUrl":"10.1111/1758-2229.70198","url":null,"abstract":"<p>Free-living amoebae are phagotrophic protists that prey on bacteria. However, under certain conditions, some bacteria can resist phagocytosis and become pathogenic. Environmental parameters altered by climate change may impact amoebae and bacterial diversity, as well as their pathogenicity. In our study, we monitored amoebae and bacterial abundance both temporally, over 1 year, and spatially, along a river estuary, while also recording key environmental parameters. <i>Naegleria</i> was the most represented amoebae genus, present year-round from the river to the estuary. Similarly, <i>Vibrio</i> was the most dominant bacterial genus. Salinity, and to a lesser extent dissolved oxygen, influenced amoebae and bacterial abundance. In particular, the genera <i>Naegleria</i>, <i>Paramoeba</i>, <i>Pseudomonas</i> and <i>Legionella</i> were the most affected. In conclusion, this study highlights the impact of salinity on amoebae diversity, suggesting that this parameter, as a key factor in coastal environments, will impact both amoebae and the associated bacterial communities.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and Spatial Survey on the Abundance of Amoebae and Bacteria in an Estuary and the Role of Environmental Parameters","authors":"Gaëlle Bednarek,&nbsp;Hélène Agogué,&nbsp;Arno Bringer,&nbsp;Tony Agion,&nbsp;Vincent Delafont,&nbsp;Yann Héchard","doi":"10.1111/1758-2229.70198","DOIUrl":"https://doi.org/10.1111/1758-2229.70198","url":null,"abstract":"<p>Free-living amoebae are phagotrophic protists that prey on bacteria. However, under certain conditions, some bacteria can resist phagocytosis and become pathogenic. Environmental parameters altered by climate change may impact amoebae and bacterial diversity, as well as their pathogenicity. In our study, we monitored amoebae and bacterial abundance both temporally, over 1 year, and spatially, along a river estuary, while also recording key environmental parameters. <i>Naegleria</i> was the most represented amoebae genus, present year-round from the river to the estuary. Similarly, <i>Vibrio</i> was the most dominant bacterial genus. Salinity, and to a lesser extent dissolved oxygen, influenced amoebae and bacterial abundance. In particular, the genera <i>Naegleria</i>, <i>Paramoeba</i>, <i>Pseudomonas</i> and <i>Legionella</i> were the most affected. In conclusion, this study highlights the impact of salinity on amoebae diversity, suggesting that this parameter, as a key factor in coastal environments, will impact both amoebae and the associated bacterial communities.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Desert in Bottles: The Culturable Microbiome of the Atacama Desert's Grit Crust","authors":"Patrick Jung,&nbsp;Laura Briegel-Williams,&nbsp;Lina Werner,&nbsp;Emily Jost,&nbsp;Rebekah Brand,&nbsp;Karen Baumann,&nbsp;Michael Lakatos","doi":"10.1111/1758-2229.70194","DOIUrl":"10.1111/1758-2229.70194","url":null,"abstract":"<p>Biological soil crusts (biocrusts) are associations of microorganisms coexisting in the top millimetres of soil, which are found predominantly in arid biomes. Recently, a new type of biocrust, termed grit crust, of the coastal range of the Atacama Desert was discovered. Here, we explore the culturable microbiome of the grit crust based on an integrative isolation approach combining morphological and phylogenetic analyses, focusing on cyanobacteria, green algae and the often overlooked non-lichenised fungi with climate records and soil data. The 122 generated isolates from four contrasting locations were distributed over three organismic groups: cyanobacteria (38), green algae (26) and non-lichenised fungi (58). The distribution of the organisms among the four locations followed a water availability gradient as shown by relative air humidity data, resulting in communities shaping the biochemistry of the substrate in terms of texture, carbon and nitrogen contents. Novel species, genera and the functional roles of the different organisms within the biocrust environment are discussed. The high abundance of endemic and presumably new species, including five potentially new genera within the cyanobacterial order of the Chroococcidiopsidales—not found in other deserts—underpins the uniqueness and further distinguishes the grit crust from other biocrusts in extreme environments.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disinfection Efficiency Among Black Alder and Hybrid Alder Genotypes and Their Influence on Alder Seed Germination In Vitro","authors":"Vytautas Čėsna,&nbsp;Ieva Čėsnienė,&nbsp;Virgilijus Baliuckas,&nbsp;Jonas Žiauka","doi":"10.1111/1758-2229.70183","DOIUrl":"10.1111/1758-2229.70183","url":null,"abstract":"<p>The presented study aimed to test a set of <i>Alnus</i> genotypes, including hybrids of black and grey alders (<i>A. glutinosa</i> × <i>A. incana</i>) and <i>A. glutinosa,</i> based on their spring phenology, using in vitro screening assays. We evaluated the disinfection efficiency and the susceptibility of the surviving fungi in different <i>Alnus</i> genotypes, comprising five with early spring phenology, four with late spring phenology and four hybrids. Then, the best-performing explants of four <i>Alnus</i> genotypes, including at least one of early and late spring phenology, as well as one hybrid, were selected to evaluate the explant influence on other <i>Alnus</i> seed germination under stress from the fungus <i>Cladosporium cladosporioides</i> inoculum. The explants of <i>Alnus</i> hybrids 047 and 026 were characterised by stronger leaf development than the <i>A. glutinosa</i> genotype 19–43–8 K, which, however, displayed the highest success of explant sterilisation. The explants of <i>A. glutinosa</i> genotype 19–43–8 K and of <i>Alnus</i> hybrid 026 were shown to increase germination of neighbouring (planted in the same test tube) <i>Alnus</i> seeds and seedling development, especially root system expansion under the stress from <i>C. cladosporioides</i>. These results point to the potential of the selected <i>Alnus</i> genotypes to relieve biotic stress in other <i>Alnus</i> seeds.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting Heavy-Metal Rhizospheres for Novel Therapeutics Against High-Priority Multi-Drug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii: A Case of Toxic to Treatment","authors":"Kylah B. Millard,&nbsp;John O. Unuofin,&nbsp;Luke Invernizzi,&nbsp;Michael O. Daramola,&nbsp;Samuel A. Iwarere","doi":"10.1111/1758-2229.70182","DOIUrl":"10.1111/1758-2229.70182","url":null,"abstract":"<p>This study investigated the antimicrobial potential of rhizospheric microbiota isolated from heavy-metal-contaminated soils against two extremely drug-resistant (XDR) pathogens, <i>Pseudomonas aeruginosa</i> (strain ATCC 27853) and <i>Acinetobacter baumannii</i> (strain ATCC-BAA-1605). Heavy-metal-contaminated rhizospheres were sequentially exposed to ex-situ and in vitro enrichment with heavy metals from battery waste and incubated for 168 h. The surviving microbiota were screened against <i>P. aeruginosa</i> and <i>A. baumannii</i>, and crude extracts of high-performing strains were tested against the pathogens using agar well diffusion assays. The novelty and components of the extracted secondary metabolites from environmentally stressed rhizospheric microorganisms were inferred using ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). Results indicated that these secretions inhibited the growth of XDR pathogens (approximately 3.0 × 10⁸ CFU/mL), forming inhibition zones of up to 50 mm. Furthermore, the pathogens were more responsive to exudates from microbiota in environmentally stressed rhizospheres compared to those from organic rhizospheres (control). Heavy-metal-stressed microbiota secrete metabolites that show superior antimicrobial activity and successfully inhibit the growth of XDR pathogens. The UPLC-HRMS analysis indicated the tentative characterisation of the metabolites, particularly <i>Tolyposamine</i> and <i>Gentiatibetine</i>, by the evaluated microbiota, suggesting their relevance as biopharmaceuticals, and could lead to future antibiotic production.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Physiological Signatures of Evolution in ANAMMOX Bacteria","authors":"Roman G. Bielski,&nbsp;M. Ahsanul Islam","doi":"10.1111/1758-2229.70197","DOIUrl":"10.1111/1758-2229.70197","url":null,"abstract":"<p>ANAMMOX bacteria are a group of strictly anaerobic bacteria that offer a sustainable and energy-saving alternative to the conventional, energy-intensive aerobic bacteria-based fixed nitrogen removal technology from wastewater treatment plants. Since their discovery, research on ANAMMOX bacteria has expanded significantly, as reflected in the growing number of publications and patents. This review examines the evolutionary and physiological adaptations that have shaped these microorganisms, drawing from genomic and physiological studies. The distinct cellular architecture and membrane composition of ANAMMOX bacteria are discussed, alongside experimental literature that assesses their activity across various temperatures and pH conditions. Genomic analyses reveal significant differences between halophilic and non-halophilic ANAMMOX bacteria, with halophiles sharing a unique set of genes absent in other species. Analysis of proteomic investigations further demonstrates evolutionary divergence, with halophilic strains exhibiting a bias toward acidic amino acids, as shown through principal component analysis. Together, these insights provide a comprehensive view of ANAMMOX bacterial evolution, linking genomic diversity to physiological adaptation.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ski Tourism Shapes the Snow Microbiome on Ski Slopes in the Italian Central Alps","authors":"Andrea Nicolò Dell'Acqua,&nbsp;Daniel Scicchitano,&nbsp;Nicola Simoncini,&nbsp;Ilaria Mercanti,&nbsp;Daniela Leuzzi,&nbsp;Silvia Turroni,&nbsp;Luca Corlatti,&nbsp;Simone Rampelli,&nbsp;Martino Colonna,&nbsp;Cinzia Corinaldesi,&nbsp;Marco Candela,&nbsp;Giorgia Palladino","doi":"10.1111/1758-2229.70195","DOIUrl":"10.1111/1758-2229.70195","url":null,"abstract":"<p>Winter sports exert significant anthropogenic pressures on the snow microbiome, affecting the entire alpine ecosystem. The massive usage of artificial snow, human occupation, and the release of xenobiotics like microplastics or ski wax components on ski tracks can profoundly alter snow microbial ecology. Here, we reconstructed the temporal dynamics of the snow microbiome at three sites in the Italian Alps: inside and outside a ski track at the impacted site of Santa Caterina Valfurva and near Cancano lake as an unimpacted control. Using epifluorescence microscopy, 16S rRNA amplicon sequencing, and inferred metagenomics, we found that the snow microbiome inside the track presented a higher load of prokaryotes and viruses. Notably, N₂-fixing microorganisms from cryospheric environments and host-associated taxa, like <i>Terrisporobacter</i>, <i>Clostridium sensu stricto</i>, <i>Enterococcus</i>, and <i>Muribaculaceae</i>, and the opportunistic pathogen <i>Citrobacter</i> characterised the impacted site. These microorganisms could originate from the river water used to produce artificial snow during winter. Our findings highlight the complexity and multifunctionality of the snow microbiome, where microorganisms with different ecological propensities can coexist, and the detectable impact of ski tourism, which enriches host-associated and xenobiotic-degrading microorganisms. This underscores the need for systematic monitoring and protection of the snow microbiome in the Alpine environment from anthropogenic threats.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary and Secondary Symbionts of Cambodian Cicadellidae and the Role of Parasitisation","authors":"Sophany Phauk,&nbsp;Lorenzo Assentato,&nbsp;Seanghun Meas,&nbsp;Olle Terenius","doi":"10.1111/1758-2229.70196","DOIUrl":"10.1111/1758-2229.70196","url":null,"abstract":"<p>Leafhoppers (Hemiptera: Cicadellidae) are important vectors of plant pathogens in agricultural systems. Biological control via parasitisation is a key management strategy, but little is known about how microbial symbionts mediate host-parasitoid interactions. Here, we characterise the bacterial communities of six Cambodian leafhopper species (<i>Cofana spectra</i>, <i>Exitianus</i> sp., <i>Goniagnathus punctifer</i>, <i>Maiestas dorsalis</i>, <i>Nephotettix virescens</i>, and <i>Stirellus</i> sp.) and their parasitoids from the families Dryinidae (Hymenoptera) and Halictophagidae (Strepsiptera). We found that the bacterial symbiont <i>Sulcia</i> dominates cicadellid microbiotas, often coexisting with secondary symbionts. For example, <i>Nasuia</i> is present alongside <i>Sulcia</i> in <i>Nephotettix</i>, while <i>Wolbachia</i> is prevalent in <i>Exitianus</i> and <i>Goniagnathus</i>. Parasitoids exhibited distinct microbiotas with greater diversity; Rhodobacteraceae and Comamonadaceae were in dryinids, while <i>Wolbachia</i> was common in Halictophagidae. We analysed the microbiota of individual pairs of host-parasitoid and although parasitism did not significantly alter cicadellid overall microbiotas, some secondary symbionts (e.g., <i>Arsenophonus</i>, <i>Wolbachia</i>, <i>Rickettsia</i>, and <i>Sodalis</i>) were detected in both hosts and parasitoids, suggesting possible microbial transmission that warrants further investigation. These findings improve our understanding of host-parasitoid microbial interactions and highlight the relationship between primary and secondary symbiont communities.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}