Environmental Microbiology Reports最新文献_第2页

Identification of ESBL-Producing Enterobacterales From Vegetable Plants: Preliminary Findings From a Small Cross-Sectional Study in a Rural Area of Madagascar 从蔬菜植物中鉴定产生esbl的肠杆菌：来自马达加斯加农村地区一项小型横断面研究的初步发现

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70130

Adrien Rieux, Mamitina Alain Noah Rabenandrasana

{"title":"Identification of ESBL-Producing Enterobacterales From Vegetable Plants: Preliminary Findings From a Small Cross-Sectional Study in a Rural Area of Madagascar","authors":"Adrien Rieux, Mamitina Alain Noah Rabenandrasana","doi":"10.1111/1758-2229.70130","DOIUrl":"https://doi.org/10.1111/1758-2229.70130","url":null,"abstract":"Extended-spectrum beta-lactamases (ESBL)-producing enterobacterales are considered a key indicator for antimicrobial resistance (AMR) epidemiological surveillance in animal, human, and environment compartments. In this study, we aim to investigate the presence and genetic diversity of ESBL-producing enterobacterales on vegetable plants. We isolated beta-lactam resistant enterobacterales from several vegetable plants and sequenced their whole genome. Utilising standard genomic and phylogenetic methods, we sought to (i) characterise the resistance genes and plasmid content of the plant-isolated strains, (ii) investigate their genetic structure, and (iii) determine their relationships with strains from other reservoirs. Among the 22 strains collected from vegetable plants, 6 showed resistance to beta-lactam antibiotics, with 5 of them identified as ESBL producers. Our results indicated the presence of multidrug-resistant (MDR) strains containing multiple antibiotic resistance genes (ARGs). Importantly, no host-specific lineages were identified among the plant-isolated ESBL-producing E. coli (ESBL-Ec). Instead, these strains exhibited genetic and epidemiological connections with strains isolated from animals, humans, and the environment, suggesting transfer of ESBL-Ec between plants and other sources in rural Madagascar. These preliminary findings suggest that vegetable plants are contaminated as a result of human activities, posing a potential risk of human and animal exposure to antibiotic-resistant bacteria and genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of ESBL-Producing Enterobacterales From Vegetable Plants: Preliminary Findings From a Small Cross-Sectional Study in a Rural Area of Madagascar 从蔬菜植物中鉴定产生esbl的肠杆菌：来自马达加斯加农村地区一项小型横断面研究的初步发现

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70130

Adrien Rieux, Mamitina Alain Noah Rabenandrasana

{"title":"Identification of ESBL-Producing Enterobacterales From Vegetable Plants: Preliminary Findings From a Small Cross-Sectional Study in a Rural Area of Madagascar","authors":"Adrien Rieux, Mamitina Alain Noah Rabenandrasana","doi":"10.1111/1758-2229.70130","DOIUrl":"https://doi.org/10.1111/1758-2229.70130","url":null,"abstract":"Extended-spectrum beta-lactamases (ESBL)-producing enterobacterales are considered a key indicator for antimicrobial resistance (AMR) epidemiological surveillance in animal, human, and environment compartments. In this study, we aim to investigate the presence and genetic diversity of ESBL-producing enterobacterales on vegetable plants. We isolated beta-lactam resistant enterobacterales from several vegetable plants and sequenced their whole genome. Utilising standard genomic and phylogenetic methods, we sought to (i) characterise the resistance genes and plasmid content of the plant-isolated strains, (ii) investigate their genetic structure, and (iii) determine their relationships with strains from other reservoirs. Among the 22 strains collected from vegetable plants, 6 showed resistance to beta-lactam antibiotics, with 5 of them identified as ESBL producers. Our results indicated the presence of multidrug-resistant (MDR) strains containing multiple antibiotic resistance genes (ARGs). Importantly, no host-specific lineages were identified among the plant-isolated ESBL-producing E. coli (ESBL-Ec). Instead, these strains exhibited genetic and epidemiological connections with strains isolated from animals, humans, and the environment, suggesting transfer of ESBL-Ec between plants and other sources in rural Madagascar. These preliminary findings suggest that vegetable plants are contaminated as a result of human activities, posing a potential risk of human and animal exposure to antibiotic-resistant bacteria and genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum 珍贵的地中海红珊瑚早期生命阶段的微生物动态

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70127

R. Tignat-Perrier, L. Bramanti, B. Giordano, J. A. J. M. van de Water, E. Manea, D. Allemand, C. Ferrier-Pagès

{"title":"Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum","authors":"R. Tignat-Perrier, L. Bramanti, B. Giordano, J. A. J. M. van de Water, E. Manea, D. Allemand, C. Ferrier-Pagès","doi":"10.1111/1758-2229.70127","DOIUrl":"https://doi.org/10.1111/1758-2229.70127","url":null,"abstract":"Microbial colonisation in the early life stages of corals plays a critical role in their fitness, but the mechanisms by which microbial symbionts are acquired—whether from parental colonies, the environment, or both—remain poorly understood, particularly in octocorals. Although they are the second most abundant coral group on tropical reefs and contribute significantly to the structural complexity of Mediterranean coral reefs, their microbial dynamics are largely unexplored. In this study, we investigated the acquisition of the bacterial microbiota in the red coral Corallium rubrum, a precious coral. By analysing the composition of the bacterial community at different early life stages, including newly released larvae, 5- and 10-day-old larvae, 3-month-old settlers, 1-year-old recruits and 3-year-old juveniles, we are gaining new insights into the development of its microbiome. Using a direct PCR-based 16S rRNA metabarcoding approach, we performed high-resolution microbiome analyses at the level of individual larvae and settlers. Our results show that the bacterial microbiota of C. rubrum matures after the first year of life. Notably, dominant symbionts, such as Spirochaetaceae and BD72BR169 Gammaproteobacteria, were absent in larvae, settlers and recruits, suggesting that they were likely acquired horizontally from the environment. These findings improve our understanding of the microbial colonisation and development of C. rubrum and shed light on the potential role of its bacterial community in holobiont function.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromium-Tanned Leather and Microbial Consortia: Identification of Taxa With Biodegradation Potential and Chromium Tolerance 铬鞣皮革与微生物群落：具有生物降解潜力和铬耐受性的分类群鉴定

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70134

Manuela Bonilla-Espadas, Irene Lifante-Martínez, Mónica Camacho, Elena Orgilés-Calpena, Francisca Arán-Aís, Marcelo Bertazzo, María-José Bonete

{"title":"Chromium-Tanned Leather and Microbial Consortia: Identification of Taxa With Biodegradation Potential and Chromium Tolerance","authors":"Manuela Bonilla-Espadas, Irene Lifante-Martínez, Mónica Camacho, Elena Orgilés-Calpena, Francisca Arán-Aís, Marcelo Bertazzo, María-José Bonete","doi":"10.1111/1758-2229.70134","DOIUrl":"https://doi.org/10.1111/1758-2229.70134","url":null,"abstract":"Chromium-tanned leather waste poses significant environmental challenges due to its resistance to degradation and heavy metal content. This study investigates the potential of naturally selected microbial consortia to initiate the degradation of chromium-tanned leather and identifies key bacterial genera capable of tolerating chromium and producing enzymes relevant to collagen breakdown. A novel multidisciplinary approach combining gravimetric assays, metagenomic sequencing, and scanning electron microscopy (SEM) was applied to characterise both microbial composition and degradation dynamics. Dominant genera such as Bacillus, Microbacterium, and Acinetobacter were associated with collagen degradation and metal tolerance, with Bacillus-rich communities showing the most pronounced mass loss (up to 3%). SEM analysis revealed the formation of robust biofilms and extensive matrix disruption, indicating enzymatic activity and structural breakdown of the leather. The formation of exopolysaccharide-rich biofilms was found to be critical for microbial adhesion and biodegradation efficacy. These findings provide initial insights into microbial mechanisms involved in the degradation of chromium-tanned leather and suggest potential applications for microbial consortia in future sustainable leather waste management strategies.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected 微氧条件下微生物生理的研究是关键但被忽视的

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-16 DOI: 10.1111/1758-2229.70108

Om Prakash, Ashvini Chauhan, Stefan J. Green

{"title":"The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected","authors":"Om Prakash, Ashvini Chauhan, Stefan J. Green","doi":"10.1111/1758-2229.70108","DOIUrl":"https://doi.org/10.1111/1758-2229.70108","url":null,"abstract":"During the early evolution of life on Earth, the environment was largely free of molecular oxygen, and only anaerobic life existed. With the subsequent oxidation of oceans and the atmosphere, a wide range of environmental niches, ranging from anoxic to microoxic/hypoxic and oxic, developed. Despite this broad range of natural environments, microbiology as a field has focused on the physiology, metabolism, and genetics of aerobic microorganisms, with less attention paid to anaerobes and much less attention paid to microaerophiles. The disparity in studies between aerobic and anaerobic conditions is rampant in host-associated systems, particularly in human health, and studies of microorganisms in intermediate oxygen conditions between fully aerobic and fully anoxic conditions are exceedingly rare. Studies on the physiological behaviour, metabolism, growth response, and drug susceptibility patterns of commensal and pathogenic organisms are almost totally neglected in microoxic conditions. Furthermore, microorganisms from microaerobic and microoxic ecosystems have been less robustly explored in terms of physiology, growth, and metabolism. In this work, we highlight the importance of understanding the physiological and metabolic behaviours of microorganisms under hypoxic or microoxic conditions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected 微氧条件下微生物生理的研究是关键但被忽视的

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-16 DOI: 10.1111/1758-2229.70108

Om Prakash, Ashvini Chauhan, Stefan J. Green

{"title":"The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected","authors":"Om Prakash, Ashvini Chauhan, Stefan J. Green","doi":"10.1111/1758-2229.70108","DOIUrl":"https://doi.org/10.1111/1758-2229.70108","url":null,"abstract":"During the early evolution of life on Earth, the environment was largely free of molecular oxygen, and only anaerobic life existed. With the subsequent oxidation of oceans and the atmosphere, a wide range of environmental niches, ranging from anoxic to microoxic/hypoxic and oxic, developed. Despite this broad range of natural environments, microbiology as a field has focused on the physiology, metabolism, and genetics of aerobic microorganisms, with less attention paid to anaerobes and much less attention paid to microaerophiles. The disparity in studies between aerobic and anaerobic conditions is rampant in host-associated systems, particularly in human health, and studies of microorganisms in intermediate oxygen conditions between fully aerobic and fully anoxic conditions are exceedingly rare. Studies on the physiological behaviour, metabolism, growth response, and drug susceptibility patterns of commensal and pathogenic organisms are almost totally neglected in microoxic conditions. Furthermore, microorganisms from microaerobic and microoxic ecosystems have been less robustly explored in terms of physiology, growth, and metabolism. In this work, we highlight the importance of understanding the physiological and metabolic behaviours of microorganisms under hypoxic or microoxic conditions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms 从盐环境中分离的三株盐杆菌的比较基因组分析揭示了潜在的耐盐性和杀藻机制

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-15 DOI: 10.1111/1758-2229.70121

Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh

{"title":"Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms","authors":"Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh","doi":"10.1111/1758-2229.70121","DOIUrl":"https://doi.org/10.1111/1758-2229.70121","url":null,"abstract":"Harmful algal blooms (HABs) pose a significant global threat to water ecosystems, prompting extensive research into their inhibition and control strategies. This study presents genomic and bioinformatic analyses to investigate the algicidal potential and elucidate the survival mechanisms in harsh conditions of newly identified Halobacillus species three strains (SSTM10-2T, SSBR10-3T, and SSHM10-5T) isolated from saline environments. Moreover, genomic and bioinformatic analyses were conducted to elucidate their survival mechanisms in harsh conditions. Moreover, comparative genomic analysis revealed a diverse set of orthologous genes, with a core genome primarily associated with metabolism and information processing. Pangenome analysis highlighted accessory and unique genes potentially involved in environmental adaptation and stress response. Functional annotation using KEGG pathways identified genes linked to xenobiotic compound degradation, stress tolerance, and salt adaptation. Additionally, the study elucidated potential mechanisms underlying algicidal activity, implicating Carbohydrate-Active enZYmes (CAZymes), cytochrome P450 oxidases (CYP), and quorum sensing (QS) systems. Finally, analysis of KEGG pathways related to microcystin degradation suggested the strains' capacity to mitigate HABs. Thus, this research enhances understanding of the genomic diversity, phylogeny, and functional characteristics of Halobacillus species, offering insights into their ecological roles and potential applications in biotechnology and environmental management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms 从盐环境中分离的三株盐杆菌的比较基因组分析揭示了潜在的耐盐性和杀藻机制

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-15 DOI: 10.1111/1758-2229.70121

Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh

{"title":"Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms","authors":"Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh","doi":"10.1111/1758-2229.70121","DOIUrl":"https://doi.org/10.1111/1758-2229.70121","url":null,"abstract":"Harmful algal blooms (HABs) pose a significant global threat to water ecosystems, prompting extensive research into their inhibition and control strategies. This study presents genomic and bioinformatic analyses to investigate the algicidal potential and elucidate the survival mechanisms in harsh conditions of newly identified Halobacillus species three strains (SSTM10-2T, SSBR10-3T, and SSHM10-5T) isolated from saline environments. Moreover, genomic and bioinformatic analyses were conducted to elucidate their survival mechanisms in harsh conditions. Moreover, comparative genomic analysis revealed a diverse set of orthologous genes, with a core genome primarily associated with metabolism and information processing. Pangenome analysis highlighted accessory and unique genes potentially involved in environmental adaptation and stress response. Functional annotation using KEGG pathways identified genes linked to xenobiotic compound degradation, stress tolerance, and salt adaptation. Additionally, the study elucidated potential mechanisms underlying algicidal activity, implicating Carbohydrate-Active enZYmes (CAZymes), cytochrome P450 oxidases (CYP), and quorum sensing (QS) systems. Finally, analysis of KEGG pathways related to microcystin degradation suggested the strains' capacity to mitigate HABs. Thus, this research enhances understanding of the genomic diversity, phylogeny, and functional characteristics of Halobacillus species, offering insights into their ecological roles and potential applications in biotechnology and environmental management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius 硝酸盐对热葡萄共生副杆菌产氢一氧化碳氧化的影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-13 DOI: 10.1111/1758-2229.70133

Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida

{"title":"Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius","authors":"Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida","doi":"10.1111/1758-2229.70133","DOIUrl":"https://doi.org/10.1111/1758-2229.70133","url":null,"abstract":"Parageobacillus thermoglucosidasius is a thermophilic facultative anaerobe capable of hydrogenogenic carbon monoxide (CO) oxidation utilising nickel-containing CO dehydrogenase (Ni-CODH) and energy-converting hydrogenase (ECH). Nitrates have been reported to exert promoting or inhibitory effects on the growth of CO oxidizers and acetogens, and these contradictory outcomes obscure the relationship between nitrate and CO oxidation. In this study, we analysed the effects of nitrate on hydrogenogenic CO oxidation and growth in P. thermoglucosidasius NBRC 107763T using wild-type and codh- and/or ech-disrupted strains. The results demonstrated that the addition of 50 mM nitrate suppressed hydrogenogenic CO oxidation while promoting hydrogen-oxidising nitrate reduction and rapid cell growth, resulting in a 2.3-fold higher OD600 than the control. Assays using cell lysates showed that 10 μM nitrate suppressed CO oxidation below the detection limit without affecting hydrogen production, indicating that nitrate affects the CO-oxidising function. These findings imply that CO oxidation in P. thermoglucosidasius is primarily coupled to proton reduction, and deactivated during nitrate respiration. Therefore, hydrogenogenic CO oxidation serves as an auxiliary energy-obtaining mechanism, functioning in the absence of alternative electron acceptors such as nitrate. This study enhances our understanding of CO-dependent energy generation and highlights the supplemental use of CO in P. thermoglucosidasius.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius 硝酸盐对热葡萄共生副杆菌产氢一氧化碳氧化的影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-13 DOI: 10.1111/1758-2229.70133

Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida

{"title":"Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius","authors":"Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida","doi":"10.1111/1758-2229.70133","DOIUrl":"https://doi.org/10.1111/1758-2229.70133","url":null,"abstract":"Parageobacillus thermoglucosidasius is a thermophilic facultative anaerobe capable of hydrogenogenic carbon monoxide (CO) oxidation utilising nickel-containing CO dehydrogenase (Ni-CODH) and energy-converting hydrogenase (ECH). Nitrates have been reported to exert promoting or inhibitory effects on the growth of CO oxidizers and acetogens, and these contradictory outcomes obscure the relationship between nitrate and CO oxidation. In this study, we analysed the effects of nitrate on hydrogenogenic CO oxidation and growth in P. thermoglucosidasius NBRC 107763T using wild-type and codh- and/or ech-disrupted strains. The results demonstrated that the addition of 50 mM nitrate suppressed hydrogenogenic CO oxidation while promoting hydrogen-oxidising nitrate reduction and rapid cell growth, resulting in a 2.3-fold higher OD600 than the control. Assays using cell lysates showed that 10 μM nitrate suppressed CO oxidation below the detection limit without affecting hydrogen production, indicating that nitrate affects the CO-oxidising function. These findings imply that CO oxidation in P. thermoglucosidasius is primarily coupled to proton reduction, and deactivated during nitrate respiration. Therefore, hydrogenogenic CO oxidation serves as an auxiliary energy-obtaining mechanism, functioning in the absence of alternative electron acceptors such as nitrate. This study enhances our understanding of CO-dependent energy generation and highlights the supplemental use of CO in P. thermoglucosidasius.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0