Environmental Microbiology Reports最新文献_第3页

The phenotypic and demographic response to the combination of copper and thermal stressors strongly varies within the ciliate species, Tetrahymena thermophila 在纤毛虫物种嗜热四膜虫中，对铜和热胁迫的表型和数量反应差异很大。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-30 DOI: 10.1111/1758-2229.13307

Doufoungognon Carine Estelle Koné, Staffan Jacob, Michèle Huet, Hervé Philippe, Delphine Legrand

{"title":"The phenotypic and demographic response to the combination of copper and thermal stressors strongly varies within the ciliate species, Tetrahymena thermophila","authors":"Doufoungognon Carine Estelle Koné, Staffan Jacob, Michèle Huet, Hervé Philippe, Delphine Legrand","doi":"10.1111/1758-2229.13307","DOIUrl":"10.1111/1758-2229.13307","url":null,"abstract":"Copper pollution can alter biological and trophic functions. Organisms can utilise different tolerance strategies, including accumulation mechanisms (intracellular vacuoles, external chelation, etc.) to maintain themselves in copper-polluted environments. Accumulation mechanisms can influence the expression of other phenotypic traits, allowing organisms to deal with copper stress. Whether copper effects on accumulation strategies interact with other environmental stressors such as temperature and how this may differ within species are still unsolved questions. Here, we tested experimentally whether the combined effect of copper and temperature modulates traits linked to demography, morphology, movement and accumulation in six strains of the ciliate Tetrahymena thermophila. We also explored whether copper accumulation might modulate environmental copper concentration effects on phenotypic and demographic traits. Results showed high intraspecific variability in the phenotypic and demographic response to copper, with interactive effects between temperature and copper. In addition, they suggested an attenuation effect of copper accumulation on the sensitivity of traits to copper, but with great variation between strains, temperatures and copper concentrations. Diversity of responses among strains and their thermal dependencies pleads for the integration of intraspecific variability and multiple stressors approaches in ecotoxicological studies, thus improving the reliability of assessments of the effects of pollutants on biodiversity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13307","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyricularia oryzae enhances Streptomyces griseus growth via non-volatile alkaline metabolites Pyricularia oryzae 通过非挥发性碱性代谢物促进灰葡萄孢链霉菌的生长。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-23 DOI: 10.1111/1758-2229.70012

Risa Sugiura, Takayuki Arazoe, Takayuki Motoyama, Hiroyuki Osada, Takashi Kamakura, Kouji Kuramochi, Yuuki Furuyama

{"title":"Pyricularia oryzae enhances Streptomyces griseus growth via non-volatile alkaline metabolites","authors":"Risa Sugiura, Takayuki Arazoe, Takayuki Motoyama, Hiroyuki Osada, Takashi Kamakura, Kouji Kuramochi, Yuuki Furuyama","doi":"10.1111/1758-2229.70012","DOIUrl":"10.1111/1758-2229.70012","url":null,"abstract":"Chemical compounds that affect microbial interactions have attracted wide interest. In this study, Streptomyces griseus showed enhanced growth when cocultured with the rice blast fungus Pyricularia oryzae on potato dextrose agar (PDA) medium. An improvement in S. griseus growth was observed before contact with P. oryzae, and no growth-promoting effect was observed when the growth medium between the two microorganisms was separated. These results suggested that the chemicals produced by P. oryzae diffused through the medium and were not volatile. A PDA plate supplemented with phenol red showed that the pH of the area surrounding P. oryzae increased. The area with increased pH promoted S. griseus growth, suggesting that the alkaline compounds produced by P. oryzae were involved in this growth stimulation. In contrast, coculture with the soilborne plant pathogen Fusarium oxysporum and entomopathogenic fungus Cordyceps tenuipes did not promote S. griseus growth. Furthermore, DL-α-Difluoromethylornithine, a polyamine biosynthesis inhibitor, prevented the increase in pH and growth promotion of S. griseus by P. oryzae. These results indicated that P. oryzae increased pH by producing a polyamine.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wolbachia modify host cell metabolite profiles in response to short-term temperature stress 沃尔巴克氏体改变宿主细胞代谢物谱，以应对短期温度胁迫。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-23 DOI: 10.1111/1758-2229.70013

Yu-Xi Zhu, Yi-Yin Zhang, Xin-Yu Wang, Yue Yin, Yu-Zhou Du

{"title":"Wolbachia modify host cell metabolite profiles in response to short-term temperature stress","authors":"Yu-Xi Zhu, Yi-Yin Zhang, Xin-Yu Wang, Yue Yin, Yu-Zhou Du","doi":"10.1111/1758-2229.70013","DOIUrl":"10.1111/1758-2229.70013","url":null,"abstract":"Wolbachia are common heritable endosymbionts that influence many aspects of ecology and evolution in various insects, yet Wolbachia-mediated intracellular metabolic responses to temperature stress have been largely overlooked. Here, we introduced the Wolbachia strain wLhui from the invasive Liriomyza huidobrensis (Blanchard) into a Drosophila Schneider 2 cell line (S2) and investigated the metabolite profile of wLhui-infected (S2_wLhui) and uninfected cell lines (S2_wu) under short-term exposure to either high (37°C), moderate (27°C), or low (7 and 17°C) temperatures. We find that Wolbachia infection, temperature stress, and their interactions significantly affect cellular metabolic profiles. Most significantly, when comparing the changes in metabolites between S2_wLhui and S2_wu, glycerophospholipids, amino acids, and fatty acids associated with metabolic pathways, microbial metabolism in diverse environments, and other pathways were significantly accumulated at either low or high temperatures. Our findings suggest Wolbachia-induced cellular physiological responses to short-term temperature stress, which may in turn affect the fitness and adaptive ability of its host as an invasive species.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ploidy levels in diverse picocyanobacteria from the Baltic Sea 波罗的海各种皮蓝藻的倍性水平

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-17 DOI: 10.1111/1758-2229.70005

Julia Weissenbach, Anabella Aguilera, Laura Bas Conn, Jarone Pinhassi, Catherine Legrand, Hanna Farnelid

{"title":"Ploidy levels in diverse picocyanobacteria from the Baltic Sea","authors":"Julia Weissenbach, Anabella Aguilera, Laura Bas Conn, Jarone Pinhassi, Catherine Legrand, Hanna Farnelid","doi":"10.1111/1758-2229.70005","DOIUrl":"https://doi.org/10.1111/1758-2229.70005","url":null,"abstract":"In nature, the number of genome or chromosome copies within cells (ploidy) can vary between species and environmental conditions, potentially influencing how organisms adapt to changing environments. Although ploidy levels cannot be easily determined by standard genome sequencing, understanding ploidy is crucial for the quantitative interpretation of molecular data. Cyanobacteria are known to contain haploid, oligoploid, and polyploid species. The smallest cyanobacteria, picocyanobacteria (less than 2 μm in diameter), have a widespread distribution ranging from marine to freshwater environments, contributing significantly to global primary production. In this study, we determined the ploidy level of genetically and physiologically diverse brackish picocyanobacteria isolated from the Baltic Sea using a qPCR assay targeting the rbcL gene. The strains contained one to four genome copies per cell. The ploidy level was not linked with phylogeny based on the identity of the 16S rRNA gene. The variation of ploidy among the brackish strains was lower compared to what has been reported for freshwater strains and was more similar to what has been reported for marine strains. The potential ecological advantage of polyploidy among picocyanobacteria has yet to be described. Our study highlights the importance of considering ploidy to interpret the abundance and adaptation of brackish picocyanobacteria.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142244955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate change and antibiotic resistance: A scoping review 气候变化与抗生素耐药性：范围审查

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-12 DOI: 10.1111/1758-2229.70008

María Fernández Salgueiro, José Antonio Cernuda Martínez, Rick Kye Gan, Pedro Arcos González

{"title":"Climate change and antibiotic resistance: A scoping review","authors":"María Fernández Salgueiro, José Antonio Cernuda Martínez, Rick Kye Gan, Pedro Arcos González","doi":"10.1111/1758-2229.70008","DOIUrl":"https://doi.org/10.1111/1758-2229.70008","url":null,"abstract":"This scoping review aimed to investigate the potential association between climate change and the rise of antibiotic resistance while also exploring the elements of climate change that may be involved. A scoping review was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews, comprehensively searching scientific literature up to 31 January 2024. Multiple databases were utilized, including MEDLINE, Web of Science and SCOPUS. Various search strategies were employed, and selection criteria were established to include articles relevant to antibiotic resistance and climate change. The review included 30 selected articles published predominantly after 2019. Findings from these studies collectively suggest that rising temperatures associated with climate change can contribute to the proliferation of antibiotic resistance, affecting diverse ecosystems. This phenomenon is observed in soil, glaciers, rivers and clinical settings. Rising temperatures are associated with a rise in the prevalence of antibiotic resistance across various environments, raising concerns for global health. However, these studies provide valuable insights but do not establish a definitive causal link between environmental temperature and antibiotic resistance. The selective pressure exerted by antibiotics and their residues in ecosystems further complicates the issue.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142231073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique episymbiotic relationship between Candidatus Patescibacteria and Zoogloea in activated sludge flocs at a municipal wastewater treatment plant 城市污水处理厂的活性污泥絮凝物中棒状杆菌与 Zoogloea 之间独特的共生关系

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-12 DOI: 10.1111/1758-2229.70007

Naoki Fujii, Kyohei Kuroda, Takashi Narihiro, Yoshiteru Aoi, Noriatsu Ozaki, Akiyoshi Ohashi, Tomonori Kindaichi

{"title":"Unique episymbiotic relationship between Candidatus Patescibacteria and Zoogloea in activated sludge flocs at a municipal wastewater treatment plant","authors":"Naoki Fujii, Kyohei Kuroda, Takashi Narihiro, Yoshiteru Aoi, Noriatsu Ozaki, Akiyoshi Ohashi, Tomonori Kindaichi","doi":"10.1111/1758-2229.70007","DOIUrl":"https://doi.org/10.1111/1758-2229.70007","url":null,"abstract":"Candidatus Patescibacteria, also known as candidate phyla radiation (CPR), including the class-level uncultured clade JAEDAM01 (formerly a subclass of Gracilibacteria/GN02/BD1-5), are ubiquitous in activated sludge. However, their characteristics and relationships with other organisms are largely unknown. They are believed to be episymbiotic, endosymbiotic or predatory. Despite our understanding of their limited metabolic capacity, their precise roles remain elusive due to the difficulty in cultivating and identifying them. In previous research, we successfully recovered high-quality metagenome-assembled genomes (MAGs), including a member of JAEDAM01 from activated sludge flocs. In this study, we designed new probes to visualize the targeted JAEDAM01-associated MAG HHAS10 and identified its host using fluorescence in situ hybridization (FISH). The FISH observations revealed that JAEDAM01 HHAS10-like cells were located within dense clusters of Zoogloea, and the fluorescence brightness of zoogloeal cells decreased in the vicinity of the CPR cells. The Zoogloea MAGs possessed genes related to extracellular polymeric substance biosynthesis, floc formation and nutrient removal, including a polyhydroxyalkanoate (PHA) accumulation pathway. The JAEDAM01 MAG HHAS10 possessed genes associated with type IV pili, competence protein EC and PHA degradation, suggesting a Zoogloea-dependent lifestyle in activated sludge flocs. These findings indicate a new symbiotic relationship between JAEDAM01 and Zoogloea.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142231072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-resolved metagenomics revealed novel microbial taxa with ancient metabolism from macroscopic microbial mat structures inhabiting anoxic deep reefs of a Maldivian Blue Hole 基因组分辨元基因组学从马尔代夫蓝洞缺氧深礁中栖息的宏观微生物垫结构中发现了具有古老新陈代谢的新型微生物类群

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-12 DOI: 10.1111/1758-2229.13315

Lapo Doni, Annalisa Azzola, Caterina Oliveri, Emanuele Bosi, Manon Auguste, Carla Morri, Carlo Nike Bianchi, Monica Montefalcone, Luigi Vezzulli

{"title":"Genome-resolved metagenomics revealed novel microbial taxa with ancient metabolism from macroscopic microbial mat structures inhabiting anoxic deep reefs of a Maldivian Blue Hole","authors":"Lapo Doni, Annalisa Azzola, Caterina Oliveri, Emanuele Bosi, Manon Auguste, Carla Morri, Carlo Nike Bianchi, Monica Montefalcone, Luigi Vezzulli","doi":"10.1111/1758-2229.13315","DOIUrl":"https://doi.org/10.1111/1758-2229.13315","url":null,"abstract":"Blue holes are vertical water-filled openings in carbonate rock that exhibit complex morphology, ecology, and water chemistry. In this study, macroscopic microbial mat structures found in complete anoxic conditions in the Faanu Mudugau Blue Hole (Maldives) were studied by metagenomic methods. Such communities have likely been evolutionary isolated from the surrounding marine environment for more than 10,000 years since the Blue Hole formation during the last Ice Age. A total of 48 high-quality metagenome-assembled genomes (MAGs) were recovered, predominantly composed of the phyla Chloroflexota, Proteobacteria and Desulfobacterota. None of these MAGs have been classified to species level (<95% ANI), suggesting the discovery of several new microbial taxa. In particular, MAGs belonging to novel bacterial genera within the order Dehalococcoidales accounted for 20% of the macroscopic mat community. Genome-resolved metabolic analysis of this dominant microbial fraction revealed a mixotrophic lifestyle based on energy conservation via fermentation, hydrogen metabolism and anaerobic CO2 fixation through the Wood–Ljungdahl pathway. Interestingly, these bacteria showed a high proportion of ancestral genes in their genomes providing intriguing perspectives on mechanisms driving microbial evolution in this peculiar environment. Overall, our results provide new knowledge for understanding microbial life under extreme conditions in blue hole environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142231024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic versatility of aerobic methane-oxidizing bacteria under anoxia in aquatic ecosystems 水生生态系统缺氧条件下需氧甲烷氧化细菌的代谢多功能性。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-04 DOI: 10.1111/1758-2229.70002

Biao Li, Zhendu Mao, Jingya Xue, Peng Xing, Qinglong L. Wu

{"title":"Metabolic versatility of aerobic methane-oxidizing bacteria under anoxia in aquatic ecosystems","authors":"Biao Li, Zhendu Mao, Jingya Xue, Peng Xing, Qinglong L. Wu","doi":"10.1111/1758-2229.70002","DOIUrl":"10.1111/1758-2229.70002","url":null,"abstract":"The potential positive feedback between global aquatic deoxygenation and methane (CH4) emission emphasizes the importance of understanding CH4 cycling under O2-limited conditions. Increasing observations for aerobic CH4-oxidizing bacteria (MOB) under anoxia have updated the prevailing paradigm that MOB are O2-dependent; thus, clarification on the metabolic mechanisms of MOB under anoxia is critical and timely. Here, we mapped the global distribution of MOB under anoxic aquatic zones and summarized four underlying metabolic strategies for MOB under anoxia: (a) forming a consortium with oxygenic microorganisms; (b) self-generation/storage of O2 by MOB; (c) forming a consortium with non-oxygenic heterotrophic bacteria that use other electron acceptors; and (d) utilizing alternative electron acceptors other than O2. Finally, we proposed directions for future research. This study calls for improved understanding of MOB under anoxia, and underscores the importance of this overlooked CH4 sink amidst global aquatic deoxygenation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of temperature on in vitro germination and growth of Colletotrichum fioriniae, a new emerging pathogen of olive fruits 温度对新出现的橄榄果病原体 Colletotrichum fioriniae 的体外发芽和生长的影响。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-09-04 DOI: 10.1111/1758-2229.13275

Federico Brugneti, Luca Rossini, Mounira Inas Drais, Silvia Turco, Angelo Mazzaglia

{"title":"Effect of temperature on in vitro germination and growth of Colletotrichum fioriniae, a new emerging pathogen of olive fruits","authors":"Federico Brugneti, Luca Rossini, Mounira Inas Drais, Silvia Turco, Angelo Mazzaglia","doi":"10.1111/1758-2229.13275","DOIUrl":"10.1111/1758-2229.13275","url":null,"abstract":"Olive anthracnose induced by different Colletotrichum species causes dramatic losses of fruit yield and oil quality. The increasing incidence of Colletotrichum fioriniae (Colletotrichum acutatum species complex) as causal agent of olive anthracnose in Italy, is endorsing new studies on its biology, ecology, and environmental factors such as temperature. Five isolates from different sampling sites in Lazio region (Central Italy) were studied under controlled laboratory conditions aiming to better understand the differences of thermal development among the isolates and to lay the foundations of a future mathematical model able to describe the key aspects of the pathogen's life cycle. The mycelial growth rate and the conidial germination rate were assessed at seven different constant temperatures (5, 10, 15, 20, 25, 30, and 35°C) and fixed relative humidity (100% RH). The obtained dataset was analysed to estimate the parameters of mathematical functions that connect the mycelial growth rate and the spore germination with the environmental temperature. The parameters set provided as the result of this study constitute a key step forward in the biological knowledge of the species and the basis for future formulations of mathematical models that might be the core of decision support systems in an integrated pest management framework.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering microbial communities involved in marine steel corrosion using high-throughput amplicon sequencing 利用高通量扩增片段测序技术解密海洋钢腐蚀过程中的微生物群落。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-08-27 DOI: 10.1111/1758-2229.70001

Biji Shibulal, Martin Peter Smith, Ian Cooper, Heidi Marie Burgess, Norman Moles, Alison Willows

{"title":"Deciphering microbial communities involved in marine steel corrosion using high-throughput amplicon sequencing","authors":"Biji Shibulal, Martin Peter Smith, Ian Cooper, Heidi Marie Burgess, Norman Moles, Alison Willows","doi":"10.1111/1758-2229.70001","DOIUrl":"10.1111/1758-2229.70001","url":null,"abstract":"To characterize the source and effects of bacterial communities on corrosion of intertidal structures, three different UK coastal sites were sampled for corrosion materials, sediment and seawater. Chemical analyses indicate the activity of sulfate-reducing microbes (SRBs) at 2 sites (Shoreham and Newhaven), but not at the third (Southend-on-Sea). Microbial communities in the deep sediment and corrosion samples are similar. The phylum Proteobacteria is dominant (40.4% of the total ASV), followed by Campilobacterota (11.3%), Desulfobacterota and Firmicutes (4%–5%). At lower taxonomic levels, corrosion causing bacteria, such as Shewanella sp. (6%), Colwellia sp. (7%) and Mariprofundus sp. (1%), are present. At Southend-on-sea, the relative abundance of Campilobacterota is higher compared to the other two sites. The mechanism of action of microorganisms at Shoreham and Newhaven involves biogenic sulfuric acid corrosion of iron by the combined action of SRBs and sulfur-oxidizing microbes. However, at Southend-on-sea, sulfur compounds are not implicated in corrosion, but SRBs and other electroactive microbes may play a role in which cathodic reactions (electrical MIC) and microbial enzymes (chemical MIC) are involved. To contribute to diagnosis of accelerated intertidal corrosion types, we developed a rapid identification method for SRBs using quantitative polymerase chain reaction high-resolution melt curve analysis of the dsrB gene.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0