Environmental Microbiology Reports最新文献_第3页

Exploring the Diversity and Function of the SPFH-Domain Containing Proteins in Pseudomonas aeruginosa. 铜绿假单胞菌含spfh结构域蛋白的多样性及功能探讨。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-04-01 DOI: 10.1111/1758-2229.70340

Víctor Manuel García-Maldonado, Claudia Rodríguez-Rangel, Dimitris Georgellis, Adrián F Alvarez

{"title":"Exploring the Diversity and Function of the SPFH-Domain Containing Proteins in Pseudomonas aeruginosa.","authors":"Víctor Manuel García-Maldonado, Claudia Rodríguez-Rangel, Dimitris Georgellis, Adrián F Alvarez","doi":"10.1111/1758-2229.70340","DOIUrl":"10.1111/1758-2229.70340","url":null,"abstract":"The SPFH-domain containing proteins are widely conserved membrane-associated factors proposed to organise membrane microdomains and thereby regulate key cellular processes. In Pseudomonas aeruginosa PA14, we identified nine such proteins (HflK, HflC and PaFlo3-PaFlo9) that display remarkable sequence divergence, genomic variability and limited phylogenetic conservation. Functional analyses of single and multiple SPFH mutants showed that these proteins are not essential for growth, biofilm formation, swimming and swarming motility, oxidative stress resistance or virulence in the Galleria mellonella infection model, although distinct slight phenotypic effects were observed in specific genetic backgrounds. Phylogenetic comparisons showed that none of the PaFlo proteins cluster with canonical bacterial flotillins such as FloA or FloT from Bacillus subtilis or FloA from Staphylococcus aureus, ruling out specific orthology. Moreover, most PaFlo proteins appear to not have clear orthologs in other γ-Proteobacteria, indicating that they may be narrowly distributed in these bacterial genomes. The absence of conserved genomic context and operon organization further supports functional diversification rather than redundancy. These findings indicate that SPFH proteins are largely dispensable for P. aeruginosa viability and virulence under laboratory conditions and likely fulfil context-dependent or niche-specific roles.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":"e70340"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147696919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cautionary Case for Host Assignment Based on Broad Environmental bla_OXA Carriers. 基于广泛环境bloxa载波的主机分配的一个警示案例。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-04-01 DOI: 10.1111/1758-2229.70327

Hanseob Shin, Min Ki Jeon, Hor-Gil Hur

{"title":"A Cautionary Case for Host Assignment Based on Broad Environmental blaOXA Carriers.","authors":"Hanseob Shin, Min Ki Jeon, Hor-Gil Hur","doi":"10.1111/1758-2229.70327","DOIUrl":"10.1111/1758-2229.70327","url":null,"abstract":"Metagenomic analyses rely heavily on contig assembly and reference databases, which can introduce substantial bias when predicting the hosts of antibiotic resistance genes (ARGs) in complex environmental microbiomes. Reference-based metagenomic pipelines assign ARGs mostly to clinically important pathogens because publicly available genomic repositories are dominated by clinically relevant isolates. Motivated by this limitation, we investigated whether metagenomic inferences accurately reflect the true bacterial hosts of ARGs in a wastewater treatment plant, also integrating culture-based validation. Metagenomic screening suggested that ARGs (blaOXA) were primarily associated with clinical taxa. In contrast, culture-based screening identified a wider host distribution of blaOXA genes. Our results imply that environmental bacteria, rather than clinically important taxa, are also hosts of blaOXA genes. Phenotypic testing showed elevated cephalosporin minimal but no carbapenem resistance, consistent with the nature of carbapenem-hydrolysing class D β-lactamases. Our findings reveal that reliance on reference-based metagenomic host prediction can underestimate the diversity of environmental ARG reservoirs. This integrated approach highlights the need for cautious interpretation of metagenomic host assignments and the importance of coupling metagenomic pipelines with culture-dependent validation when assessing ARG ecology in the natural environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":"e70327"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13066498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards a Novel Biocontrol Strategy: High Performance of Optimised Cell Wall-Degrading Enzymes Secreted by Escovopsis primorosea LBM 277. 迈向一种新的生物防治策略：经优化的原叶蛾LBM 277分泌的细胞壁降解酶的高效能。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-04-01 DOI: 10.1111/1758-2229.70271

Marcela Paola Barengo, Natalia Soledad Amerio, Gustavo Ángel Bich, Pedro Darío Zapata, María Lorena Castrillo

{"title":"Towards a Novel Biocontrol Strategy: High Performance of Optimised Cell Wall-Degrading Enzymes Secreted by Escovopsis primorosea LBM 277.","authors":"Marcela Paola Barengo, Natalia Soledad Amerio, Gustavo Ángel Bich, Pedro Darío Zapata, María Lorena Castrillo","doi":"10.1111/1758-2229.70271","DOIUrl":"10.1111/1758-2229.70271","url":null,"abstract":"Fungi of the genus Escovopsis are specialised mycoparasites of the mutualistic fungus Leucoagaricus gongylophorus, cultivated by leaf-cutter ants. Harnessing this natural antagonism represents a promising and environmentally friendly strategy for the indirect biological control of these agroforestry pests. This study optimised and characterised the production of cell wall-degrading enzymes (CWDEs), key effectors in the degradation of the host, by the Escovopsis primorosea LBM 277 strain. Using submerged fermentation and Box-Behnken response surface methodology (RSM-BBD), we identified optimal culture conditions, including the concentrations of carbon and nitrogen sources, initial pH and inoculum size. Under optimised conditions, protease, β-1,3-glucanase and chitinase activities increased by 3.5-, 6.7- and 6.8-fold, respectively. The enzymes remained active for at least 30 days at room temperature and in the pH range 4-6, resembling the microenvironment of ant fungal gardens. Zymographic analysis revealed one isoform of protease, one of β-1,3-glucanase and two chitinase isoenzymes. This is the first report combining RSM-based optimization with biochemical profiling of CWDEs in Escovopsis. These findings highlight the need for further functional assays to validate the role of these enzymes in the ant-symbiont fungus-mycoparasite interaction.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":"e70271"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli Nissle 1917 Occupies Previously Undocumented Host Niches in the Insect-Parasitic Nematode Steinernema hermaphroditum. 大肠杆菌Nissle 1917占据了以前未记载的寄主生态位-寄生线虫斯坦纳马雌雄同体。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-04-01 DOI: 10.1111/1758-2229.70326

Victoria Chen, John P Marken, Richard M Murray, Mengyi Cao

{"title":"Escherichia coli Nissle 1917 Occupies Previously Undocumented Host Niches in the Insect-Parasitic Nematode Steinernema hermaphroditum.","authors":"Victoria Chen, John P Marken, Richard M Murray, Mengyi Cao","doi":"10.1111/1758-2229.70326","DOIUrl":"10.1111/1758-2229.70326","url":null,"abstract":"Steinernema species are soil-dwelling, insect-parasitic nematodes that maintain species-specific associations with Xenorhabdus symbiotic bacteria, which are packaged within anterior intestinal pockets during the infective juvenile (IJ) stage. While these nematodes can persist in soil for months while seeking insect hosts, their interactions with environmental microbes beyond their native symbionts remain poorly understood. Here, we describe a previously uncharacterized interaction between Escherichia coli Nissle 1917 (EcN) and Steinernema hermaphroditum. EcN cells are enclosed and lysed within multiple pairs of putative coelomocytes, suggesting microbial endocytosis by host cells. During the IJ stage, EcN localizes to posterior intestinal compartments and the inter-cuticular space, where cells proliferate, aggregate and subsequently lyse. Bacterially expressed proteins persist within the nematode cuticle for over 8 weeks in non-sterile soil. These findings reveal sequential stages of environmental bacterial colonization associated with host immune responses distinct from mutualistic symbiosis. This work establishes a model for understanding nematode and environmental microbe interactions and highlights opportunities to deliver bacterially expressed molecules for environmental biosensing and biocontrol applications.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":"e70326"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13052097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Amplicon and Shotgun Metagenome Profiling of Soil Microbial Communities in Kauri Forests Affected by Phytophthora agathidicida. 受疫霉影响的杉木林土壤微生物群落的扩增子和散弹枪宏基因组比较分析

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-04-01 DOI: 10.1111/1758-2229.70324

Zoe King, Hannah L Buckley, Gavin Lear, Brent Seale, Kevin C Lee, Luitgard Schwendenmann, Donnabella C Lacap-Bugler

{"title":"Comparative Amplicon and Shotgun Metagenome Profiling of Soil Microbial Communities in Kauri Forests Affected by Phytophthora agathidicida.","authors":"Zoe King, Hannah L Buckley, Gavin Lear, Brent Seale, Kevin C Lee, Luitgard Schwendenmann, Donnabella C Lacap-Bugler","doi":"10.1111/1758-2229.70324","DOIUrl":"10.1111/1758-2229.70324","url":null,"abstract":"Soil-borne pathogens can influence microbial communities and ecosystem function, making it important to understand their broader ecological impacts. We investigated interactions between Phytophthora agathidicida (the causal agent of kauri tree dieback) and soil microbial communities, while also comparing detection and community-profiling methods. Soils from 60 kauri trees across three sites in the Waitākere Ranges, New Zealand, were analysed using loop-mediated isothermal amplification (LAMP) for pathogen detection, and 16S rRNA gene/ITS gene amplicon sequencing alongside shotgun metagenomics for community characterisation. LAMP detected P. agathidicida in 39/60 samples, while shotgun sequencing detected Phytophthora-associated DNA at low abundance across all samples. Microbial community structure and functional potential showed weak association with pathogen presence, though differential abundance testing identified several genera enriched in pathogen-detected soils, including taxa previously linked to disease suppression. Amplicon and shotgun profiles indicated broadly comparable patterns at higher taxonomic and functional levels, while differences between approaches emerged primarily at finer taxonomic resolution. Importantly, functional predictions from PICRUSt2 closely matched shotgun-derived profiles at broader scales, indicating its suitability as a cost-effective tool for broad-scale monitoring. These findings suggest limited direct pathogen effects on microbial communities and highlight how integrating molecular approaches provides complementary insights into soil microbiome-pathogen interactions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":"e70324"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147588969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Diversity of Cyanotoxins and Retinoid Compounds in Antarctic Microbial Mats: Evidence From James Ross Island 南极微生物垫中蓝藻毒素和类维生素a化合物的发生和多样性：来自詹姆斯罗斯岛的证据。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-31 DOI: 10.1111/1758-2229.70321

Luděk Sehnal, Lucie Bláhová, Marie Smutná, Ondřej Mikeš, Pavel Babica, Klára Hilscherová

{"title":"Occurrence and Diversity of Cyanotoxins and Retinoid Compounds in Antarctic Microbial Mats: Evidence From James Ross Island","authors":"Luděk Sehnal, Lucie Bláhová, Marie Smutná, Ondřej Mikeš, Pavel Babica, Klára Hilscherová","doi":"10.1111/1758-2229.70321","DOIUrl":"10.1111/1758-2229.70321","url":null,"abstract":"Antarctic ecosystems, though extreme, harbour diverse microbial communities dominated by cyanobacteria, which serve as crucial primary producers. Whilst the occurrence of cyanotoxins has been extensively studied in temperate climates, limited research has focused on their presence in polar regions. In this study, we investigated the distribution and diversity of cyanotoxins across various aquatic ecosystems on James Ross Island, Antarctica. Using targeted LC–MS/MS analysis, we detected microcystins, nodularin, cylindrospermopsin, and, for the first time in the Antarctic region, the neurotoxin saxitoxin, albeit at concentrations below quantifiable levels. Our findings also reveal the widespread presence of retinoids in microbial mats, with potential endocrine-disruptive properties, marking the first report of these metabolites in Antarctic ecosystems. This study highlights the ecological importance of cyanobacterial metabolites in Antarctic environments and raises concerns about their potential effects on local wildlife and water quality, particularly in the context of climate change. The findings underscore the need for continued monitoring and molecular studies to elucidate the sources and ecological roles of cyanotoxins in polar regions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147588982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity-Informed Network Analysis Reveals Keystone Microbes Shaping Freshwater Ecosystem Function 活动信息网络分析揭示关键微生物塑造淡水生态系统功能。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-19 DOI: 10.1111/1758-2229.70245

Qiyao Yang, Rosa Aghdam, Patricia Q. Tran, Karthik Anantharaman, Claudia Solís-Lemus

{"title":"Activity-Informed Network Analysis Reveals Keystone Microbes Shaping Freshwater Ecosystem Function","authors":"Qiyao Yang, Rosa Aghdam, Patricia Q. Tran, Karthik Anantharaman, Claudia Solís-Lemus","doi":"10.1111/1758-2229.70245","DOIUrl":"10.1111/1758-2229.70245","url":null,"abstract":"Freshwater lakes are dynamic ecosystems, with varying oxygen dynamics that influence microbiome structure, composition, and transcriptomic activity. In many freshwater studies, ecological function and abundance metrics are used to discover keystone species; however, it is well established that abundance does not equal activity. Despite the existence of long-term time series spanning multiple years, no previous study has looked at how microbial community and activity (metatranscriptomics) are influenced by shifting oxygen conditions across depths at the microbial network level. In this study, we leverage metagenome-assembled genomes and transcriptomic activity to identify keystone taxa in the ecosystem. Using the SPIEC-EASI and CARlasso methods, we mapped key microbial associations and used permutation-based analyses to assess the robustness of keystone identification. Our results reveal that a taxon's ecological centrality is context-dependent and that many species identified as keystone by abundance alone do not exhibit corresponding transcriptional activity. Notably, members of Bacteroidota and other lineages emerged as keystone taxa only when both abundance and activity were considered. Our study underscores the importance of combining metagenomic and metatranscriptomic approaches for accurate identification of functionally relevant keystone species in freshwater ecosystems, providing a framework for future microbial ecology studies.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Hidden Drivers: How Vegetation Cover, Season and Forest Management Shape the Soil Microbial Community in Two Mediterranean Forest Ecosystems 揭示隐藏的驱动因素：植被覆盖、季节和森林管理如何塑造两个地中海森林生态系统中的土壤微生物群落。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-19 DOI: 10.1111/1758-2229.70255

Enrica Picariello, Flavia De Nicola

{"title":"Unveiling the Hidden Drivers: How Vegetation Cover, Season and Forest Management Shape the Soil Microbial Community in Two Mediterranean Forest Ecosystems","authors":"Enrica Picariello, Flavia De Nicola","doi":"10.1111/1758-2229.70255","DOIUrl":"10.1111/1758-2229.70255","url":null,"abstract":"Soil provides essential ecosystem services and serves as a habitat for biodiversity, but it is often affected by disturbances from management practices and seasonal changes, which can alter its microbial communities. This study investigated the combined effects of dominant vegetation, forest management, and seasonal variation on soil microbial communities and enzyme activity over one year in turkey oak and beech forests managed as high forest or coppice. Results showed that the dominant vegetation type had a greater influence on microbial communities than seasonal changes. While forest management did not significantly affect microbial activity, it altered microbial community composition. In beech forests, bacterial communities (at the order level) showed relative abundances higher in soil under high forest with respect to coppice, whereas the fungal community showed orders most abundant under coppice management with respect to the high forest. Forest management changed the relative abundances of microbial communities, but it did not remarkably affect microbial community functions and, thus, the associated ecosystem services. Our results highlight that the forest type should be considered when evaluating forest management. This study offers new insights into the factors influencing the composition of soil microbial communities and their associated ecosystem functions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Plant Growth Promotion Potential of Novel Compost-Derived Bacilli and Anticipating Their In Vivo Effectiveness Through Mathematical Modelling 新型堆肥芽孢杆菌促进植物生长潜力的研究及其体内有效性的数学模型预测。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-11 DOI: 10.1111/1758-2229.70308

Debapriya Maitra, Bedaprana Roy, Bikram Dhara, Archisman Chakraborti, Indranath Chaudhuri, Ranadhir Chakraborty, Sudeshna Shyam Choudhury, Swastika Maitra, Ajoy Kumer, Arup Kumar Mitra

{"title":"Investigation of Plant Growth Promotion Potential of Novel Compost-Derived Bacilli and Anticipating Their In Vivo Effectiveness Through Mathematical Modelling","authors":"Debapriya Maitra, Bedaprana Roy, Bikram Dhara, Archisman Chakraborti, Indranath Chaudhuri, Ranadhir Chakraborty, Sudeshna Shyam Choudhury, Swastika Maitra, Ajoy Kumer, Arup Kumar Mitra","doi":"10.1111/1758-2229.70308","DOIUrl":"10.1111/1758-2229.70308","url":null,"abstract":"Concerns about the unintended consequences of using pesticides in agricultural settings have led to a shift in practise towards more environmentally friendly methods such as composting, manuring and using plant growth-promoting bacteria. While compost is known for its ability to promote plant growth and serve as a soil amendment, little is known about the various types of bacteria that can be found in it and how effective they are in sustainable agriculture. This study was designed to assess the potency of a few common isolates from local compost by quantifying their capacity to fix nutrients, produce growth hormone, mitigate abiotic stress, produce lignocellulose-degrading enzyme and produce soil-amending enzyme, all while scaling them using an objective in silico method. In addition, the isolates were tested in the field against test crops selected from the monocot and dicot families, namely maize (Zea mays) and okra (Abelmoschus esculentus). When comparing the treated settings to the untreated control setups, an increase was seen in the quantity of fruits (okra) and percentage of grain filling (maize). By creating a score system, an attempt was made to compare the efficiency of five strains based on the in vitro and in vivo experiments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil and Genotype Shape the Sugarcane Phytobiome for Enhanced Environmental Adaptation 土壤和基因型塑造甘蔗植物群落以增强环境适应性。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-11 DOI: 10.1111/1758-2229.70314

J. D. Ferreti, B. Ribeiro, J. de A. Bonetti, L. E. A. Camargo, S. Creste, E. E. Kuramae, C. B. Monteiro-Vitorello

{"title":"Soil and Genotype Shape the Sugarcane Phytobiome for Enhanced Environmental Adaptation","authors":"J. D. Ferreti, B. Ribeiro, J. de A. Bonetti, L. E. A. Camargo, S. Creste, E. E. Kuramae, C. B. Monteiro-Vitorello","doi":"10.1111/1758-2229.70314","DOIUrl":"10.1111/1758-2229.70314","url":null,"abstract":"Soil properties critically shape sugarcane growth and its microbiome, yet their influence on gene expression remains unclear. We investigated the combined effects of soil type (clayey and sandy loam) and sugarcane genotype (IACSP-5503 and IACSP-6007) on microbiome composition and plant transcriptional profiles. Bacterial communities from soils and stalk tissues, as well as transcriptomes of 48-h sprouted buds grown for 10 months, were analysed. Results showed that IACSP-5503 (adapted to low-fertility soils) and IACSP-6007 (less adapted) recruited endophytic microbiota in a soil–genotype-dependent manner. In sandy loam, IACSP-5503 promoted diverse plant growth-promoting bacteria (PGPB) (including Burkholderia, Leifsonia and Mycobacterium), associated with nitrogen fixation, hormone production and stress tolerance, while IACSP-6007 displayed reduced PGPB diversity and transcriptomic signatures of nutrient deficiencies. Conversely, in clayey soil, IACSP-6007 recruited more PGPBs (such as Pseudomonas, Bacillus and Klebsiella) linked to nutrient acquisition and defence responses. Both genotypes exhibited enhanced expression of defence- and antioxidant-related genes in clayey soil, suggesting priming effects. Overall, our findings reveal soil-dependent, genotype-specific microbial recruitment strategies, particularly in IACSP-5503, reflecting adaptive responses to nutrient-poor conditions. The combined 16S metataxonomic and transcriptome data offered insights into how soil and genotype shape microbial recruitment and transcriptional plasticity in sugarcane.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0