Environmental Microbiology Reports最新文献_第4页

Transcriptome Analysis Reveals Cross-Kingdom Virulence Factors in Erwinia persicina Cp2 猪Erwinia persicina Cp2转录组分析揭示跨界毒力因子。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-11 DOI: 10.1111/1758-2229.70322

Rong Huang, Xiao-Ni Liu, Tom Hsiang, Zhen-Fen Zhang

{"title":"Transcriptome Analysis Reveals Cross-Kingdom Virulence Factors in Erwinia persicina Cp2","authors":"Rong Huang, Xiao-Ni Liu, Tom Hsiang, Zhen-Fen Zhang","doi":"10.1111/1758-2229.70322","DOIUrl":"10.1111/1758-2229.70322","url":null,"abstract":"Erwinia persicina is a well-documented plant pathogenic bacterium, causing soft rot in various plant hosts. There are rare previous reports that it is associated with animal diseases. In this study, we used an alfalfa root infection model and a mouse model via gavage administration of E. persicina strain Cp2. Strain Cp2 was pathogenic on both alfalfa and mice. On alfalfa, Cp2 primarily caused leaf spot and etiolation. Mice exhibited moderate lesions in their liver, stomach and kidney tissues, with inflammation in the liver and kidney after gavage. Furthermore, viable Cp2 cells were successfully reisolated from mouse faeces at different time periods after gavage. Transcriptome analysis revealed that the 34 bacterial differentially expressed genes (DEGs) in Cp2 in response to infecting both alfalfa and mice were predominantly enriched in pathways related to bacterial adhesion, biofilm and motility. Bap was identified as a key virulence gene contributing to cross-kingdom pathogenicity of Cp2. The results provide a solid foundation for the subsequent functional validation of the virulence factors of this cross-kingdom pathogenic bacterium.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70322","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Analysis of IAA Anabolism Pathway Based on Bacillus cereus, Bacillus subtilis and Bacillus safensis Genome 基于蜡样芽孢杆菌、枯草芽孢杆菌和萨芬芽孢杆菌基因组的IAA合成代谢途径差异分析。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-11 DOI: 10.1111/1758-2229.70323

Dongyue Hou, Xuejie Zhai, Kezhong Zhang, Jinteng Cui

{"title":"Differential Analysis of IAA Anabolism Pathway Based on Bacillus cereus, Bacillus subtilis and Bacillus safensis Genome","authors":"Dongyue Hou, Xuejie Zhai, Kezhong Zhang, Jinteng Cui","doi":"10.1111/1758-2229.70323","DOIUrl":"10.1111/1758-2229.70323","url":null,"abstract":"Bacteria of the genus Bacillus are widely used in agriculture due to their ability to synthesise IAA, but the metabolic pathways of IAA synthesis and the regulatory mechanisms of key genes remain unclear. In this study, the biochemical characteristics of Bacillus cereus, Bacillus subtilis and Bacillus safensis were detected. It was found that Bacillus subtilis has a higher indoles synthesis efficiency, Bacillus safensis has stronger salt tolerance and Bacillus cereus has more prominent alkali tolerance. Through whole-genome sequencing and comparative analysis, combined with the retrieval of key genes in the tryptophan metabolic pathway and the metabolic analysis of indole compounds, it is clarified that Bacillus cereus has three IAA synthesis pathways, namely TAM, IAM and IPyA; Bacillus subtilis contains IAM and IPyA pathways; and Bacillus safensis only has the IPyA pathway. This study offers new insights into Bacillus metabolism, high-quality strains for microbial agents, and matters for plant-growth bacteria application and agricultural sustainability.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhizobacteria Exopolysaccharide: A Boon in Reclaiming Soil Fertility, Augmenting Plant Growth and Plant Stress Resilience 根杆菌胞外多糖：恢复土壤肥力、促进植物生长和植物抗逆性的一种有利物质。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-11 DOI: 10.1111/1758-2229.70296

Aishmita Gantait, Sam A. Masih, Ann Maxton, Adriano Sofo, Rosangela Addesso

{"title":"Rhizobacteria Exopolysaccharide: A Boon in Reclaiming Soil Fertility, Augmenting Plant Growth and Plant Stress Resilience","authors":"Aishmita Gantait, Sam A. Masih, Ann Maxton, Adriano Sofo, Rosangela Addesso","doi":"10.1111/1758-2229.70296","DOIUrl":"10.1111/1758-2229.70296","url":null,"abstract":"Microbial exopolysaccharides (EPSs) serve multiple industrial and environmental purposes operating as complex biopolymers produced by bacteria and fungus, as well as by yeast and microalgae. The structural diversity of microbial EPS enables the biofilm formation, the stress resistance and the nutrient storage, comprising homopolysaccharides and heteropolysaccharides. Soil structure receives substantial improvement through EPS because the polymers help aggregate particles, retaining more water and trapping heavy metals, that results in enhanced soil fertility useful in sustainable agricultural practices. Moreover, through the presence of EPS-producing bacteria, plants can establish beneficial connections with microorganisms that improve their tolerance to environmental factors, including salt exposure, drought conditions and extreme temperature changes. Such polymers find applications in the bioremediation and pharmaceutical fields because they present significant pharmacological properties, such as antibacterial, anti-inflammatory activities and antioxidant behaviour. Their biodegradable nature and eco-friendly properties make it eligible as a sustainable choice to replace synthetic polymers. This paper broaches the multiple ways how EPS improves plant wellness and enhances soil quality. Potential solutions emerge from microbial EPS research to address global challenges in agricultural sectors, biotechnological fields, and environmental management domains.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Specialisation Shapes Gut Bacterial Diversity in Dung Beetles: Insights From Coprophagy to Millipede Carnivory 饮食专业化塑造了屎壳郎肠道细菌的多样性：从食粪到千足虫食肉性的见解。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-10 DOI: 10.1111/1758-2229.70317

Johann C. de Beer, Kazeem A. Alayande, Christian W. W. Pirk, Rasheed A. Adeleke, Catherine L. Sole

{"title":"Dietary Specialisation Shapes Gut Bacterial Diversity in Dung Beetles: Insights From Coprophagy to Millipede Carnivory","authors":"Johann C. de Beer, Kazeem A. Alayande, Christian W. W. Pirk, Rasheed A. Adeleke, Catherine L. Sole","doi":"10.1111/1758-2229.70317","DOIUrl":"10.1111/1758-2229.70317","url":null,"abstract":"Dung beetles are ecosystem engineers, providing ecosystem services like nutrient cycling, waste degradation and parasite suppression. Their gut microbiome is essential for exploiting specialised diets, yet the eco-evolutionary factors driving microbial composition across diverse feeding strategies remain ambiguous. Here, we show that diet strongly influences gut bacterial composition across seven dung beetle species specialising in coprophagy, necrophagy, detritophagy, fungivory and carnivory. Most dietary specialisations grouped separately, though fungivores clustered with carrion and millipede feeders. The millipede-feeding species, Sceliages brittoni and S. hippias, hosted the most distinct and least diverse gut microbiomes. Taxonomically, differences were driven by distinct marker taxa, many of which are consistently isolated across taxonomic orders with similar diets. For example, the indicative bacterial species I. indica has been identified in various flesh-feeding insect taxa. Crucially, this pattern of shared bacterial communities suggests that diet is a dominant structuring factor which promotes community convergence regardless of host phylogeny. This study highlights the role of diet in shaping the dung beetle gut microbiome and provides the first characterisation of the gut microbiota in millipede-feeding dung beetles. Our findings underscore the critical role of diet, laying the foundation for functional studies into the eco-evolutionary significance of these host–microbe interactions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Foundation for Advancing Studies of the Biodegradation of Polyethylene Surrogates by Environmental and Model Laboratory Microbes 环境和模型实验室微生物降解聚乙烯代物的研究进展基础

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-10 DOI: 10.1111/1758-2229.70319

Bilge Bahar Camur, Lucas Stolp, Lydia Mindermann, Natalia Calixto Mancipe, Joseph B. Moidl, Brett M. Barney

{"title":"A Foundation for Advancing Studies of the Biodegradation of Polyethylene Surrogates by Environmental and Model Laboratory Microbes","authors":"Bilge Bahar Camur, Lucas Stolp, Lydia Mindermann, Natalia Calixto Mancipe, Joseph B. Moidl, Brett M. Barney","doi":"10.1111/1758-2229.70319","DOIUrl":"10.1111/1758-2229.70319","url":null,"abstract":"Polyethylene represents a particularly recalcitrant class of plastics that persist for decades in the natural environment when released as the result of failed waste management policies. In this report, we present a detailed survey of microbes with varying abilities to degrade either branched or linear waxy hydrocarbons that serve as a surrogate for the study of polyethylene biodegradation. This analysis includes measurement of the degree of branching for the surrogates. We further monitored the growth of individual isolates as an indication of substrate preference. We sequenced the genomes for each of our isolates that showed significant rates of growth to accommodate future biochemical studies, and provide a general characterisation of each strain. The vast majority of microbes that we isolated and identified as part of this study were Actinomycetes. However, a small selection of gram-negative microbes were identified that resulted in degradation of the surrogates. Importantly, our results further identified the model microbes Acinetobacter baylyi and Rhodococcus jostii as strains that were particularly good at degrading all three of the model polyethylene surrogates employed in this study. The results of this study should serve as a detailed genetic and biochemical resource to the research community investigating polyethylene biodegradation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions of Mucus Monosaccharides and the Epidermal Microbiome in Four Benthic Elasmobranchs 四种底栖动物黏液单糖与表皮微生物群的相互作用。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-07 DOI: 10.1111/1758-2229.70303

Emma N. Kerr, Long Yu, Ryan D. Hesse, Chloe N. Roberts, Vincent Bulone, Lauren Meyer, Robert A. Edwards, Michael P. Doane, Elizabeth A. Dinsdale

{"title":"Interactions of Mucus Monosaccharides and the Epidermal Microbiome in Four Benthic Elasmobranchs","authors":"Emma N. Kerr, Long Yu, Ryan D. Hesse, Chloe N. Roberts, Vincent Bulone, Lauren Meyer, Robert A. Edwards, Michael P. Doane, Elizabeth A. Dinsdale","doi":"10.1111/1758-2229.70303","DOIUrl":"10.1111/1758-2229.70303","url":null,"abstract":"Epidermal mucus is a complicated mixture of macromolecules which acts as the first line of defence for organisms against abrasions and infections. We quantified the carbohydrate (monosaccharide) composition of the mucus from four Elasmobranchii hosts, including eagle rays (Myliobatis tenuicaudatus), Port Jackson sharks (Heterodontus portusjacksoni), Australian angelsharks (Squatina australis) and whitespotted skates (Dentiraja cerva). Elasmobranchii had low amounts of mucus and a low proportion of carbohydrates (< 10%) compared with other marine organisms. Four key monosaccharides: glucose, glucosamine, galactose and fucose, were identified in mucus samples. Hosts exhibited distinct, species-specific monosaccharide signatures. We identified key carbohydrate microbial genes from host and water microbiomes. Elasmobranch microbiomes had a higher relative abundance of carbon utilisation genes compared to the water column and contained gene pathways for the utilisation of specific monosaccharides found in host mucus, suggesting that the host mucus was a regulator of the microbiome. Elasmobranch epidermal microbiomes have the genetic machinery required for detecting, transporting and metabolising monosaccharides and other carbohydrates present in the host mucus, demonstrating the selective nature of Elasmobranch epidermal mucus.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147371969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and Plant Growth-Promoting Potential of Duckweed-Associated Bacteria on Wolffia globosa Biomass Production and Nutritional Quality 浮萍伴生菌多样性及其对全球沃尔夫生物量产量和营养品质的促进作用

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-07 DOI: 10.1111/1758-2229.70312

Sirapat Kettongruang, Masaaki Morikawa, Chanita Boonmak

{"title":"Diversity and Plant Growth-Promoting Potential of Duckweed-Associated Bacteria on Wolffia globosa Biomass Production and Nutritional Quality","authors":"Sirapat Kettongruang, Masaaki Morikawa, Chanita Boonmak","doi":"10.1111/1758-2229.70312","DOIUrl":"10.1111/1758-2229.70312","url":null,"abstract":"Wolffia (Lemnoideae) is recognised as a nutritional superfood with increasing interest in commercial cultivation. Its growth and biomass quality are influenced by abiotic factors and duckweed-associated bacteria (DAB) that support nutrient cycling, stress tolerance and metabolism. This study assessed DAB diversity and their effects on Wolffia growth to select effective plant growth-promoting bacteria (PGPB) and elucidated beneficial plant-microbe interactions. A total of 108 isolates, representing 66 species from 41 genera across four phyla, were obtained from duckweeds collected in six provinces of Thailand. The culturable DAB community showed high taxonomic diversity, dominated by the phylum Pseudomonadota, particularly Alphaproteobacteria. The isolates, along with the known PGPB of Spirodela polyrhiza, were evaluated for growth promotion in axenic Wolffia globosa using co-cultivation. Six PGPB enhanced growth by 54.67%–77.75% without reported pathogenicity. Several PGPB originally isolated from S. polyrhiza also showed growth-promoting effects on Wolffia. Large-scale co-cultivation demonstrated that Pseudomonas toyotomiensis W5–11 most effectively increased dry weight and chlorophyll content (3.18- and 2.75-fold, respectively). All selected isolates produced indole-3-acetic acid (IAA), siderophores and solubilised phosphate. Correlation analysis revealed that IAA production was associated with protein accumulation. These findings suggested that PGPB represent promising biofertilisers that enhance Wolffia productivity and nutritional quality through various mechanisms.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147371909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pollinator Visitation Alters Cranberry Flower Fungal Communities in Wisconsin Cranberry Agroecosystems 传粉者访问改变威斯康星州蔓越莓农业生态系统的蔓越莓花真菌群落。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-06 DOI: 10.1111/1758-2229.70310

Celeste C. Mezera, Shawn Steffan, Leslie A. Holland

{"title":"Pollinator Visitation Alters Cranberry Flower Fungal Communities in Wisconsin Cranberry Agroecosystems","authors":"Celeste C. Mezera, Shawn Steffan, Leslie A. Holland","doi":"10.1111/1758-2229.70310","DOIUrl":"10.1111/1758-2229.70310","url":null,"abstract":"Pollinators are known dispersal agents of microbial communities between flowering plants, although the role of insect-mediated microbial assembly in flowering agricultural crops is not well understood. In cranberry (Vaccinium macrocarpon Ait.) agroecosystems, the blossom period is a vulnerable time for infection from pathogens within the cranberry fruit rot fungal disease complex, and understanding the components and assembly dynamics in cranberry flower fungal communities may provide important insights to the relationship between the cranberry microbiome and crop health. This 2-year study uses a combination of culture-dependent and next-generation sequencing approaches to compare the community structure of cranberry flowers, honey bees (Apis mellifera), bumble bees (Bombus sp.), wild solitary bees, hover flies (Syrphidae), and nearby wildflowers to identify shared fungal associates. Compared to a tenting treatment in cranberry flowers used to prevent insect visitation, cranberry flowers with access to pollinators have higher detection of fungal genera in culture, as well as more fungal genera identified through culture-independent methods. Fungi associated with the cranberry fruit rot complex were identified in several insect groups, with the highest proportion of identified fruit rot fungi detected in Toxomerus fly samples. This research provides the first evidence of shared fungal communities between pollinators and managed cranberry flowers.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Fire and Heat Stress on Soil Microorganisms: A Review of Community Changes, Molecular Responses and Plant-Beneficial Roles 火和热胁迫对土壤微生物的影响：群落变化、分子响应和植物有益作用综述

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-05 DOI: 10.1111/1758-2229.70247

Ma. del Carmen Orozco-Mosqueda, Aurora Flores-Piña, Ajay Kumar, Fannie I. Parra-Cota, Sergio de los Santos-Villalobos, Debasis Mitra, Olubukola Oluranti Babalola, Gustavo Santoyo

{"title":"Impact of Fire and Heat Stress on Soil Microorganisms: A Review of Community Changes, Molecular Responses and Plant-Beneficial Roles","authors":"Ma. del Carmen Orozco-Mosqueda, Aurora Flores-Piña, Ajay Kumar, Fannie I. Parra-Cota, Sergio de los Santos-Villalobos, Debasis Mitra, Olubukola Oluranti Babalola, Gustavo Santoyo","doi":"10.1111/1758-2229.70247","DOIUrl":"10.1111/1758-2229.70247","url":null,"abstract":"Fire, whether occurring on the surface or underground, significantly influences soil microbial dynamics by reshaping community composition, functional diversity and overall soil and plant health. This review examines the effects of fire on soil-beneficial microbial communities, with particular attention to how surface and underground fires drive shifts in microbial diversity and functional roles within the agroecosystems. These changes impact key processes such as nutrient cycling, soil physicochemical structure and organic matter decomposition, ultimately affecting crop production. Bacterial groups such as Firmicutes and Actinobacteria often increase in abundance following fire events, while others lacking survival strategies tend to decline. Resilient fungal groups, including Ascomycota (such as Aspergillus, Penicillium and Trichoderma), frequently play pivotal roles during the recovery process. Fire can also enhance microbial metabolic activity, particularly in pathways involved in organic matter degradation, leading to short-term increases in nutrient availability that support plant regrowth. Finally, the review discusses the molecular responses of microbes to fire and outlines perspectives for better understanding this type of stress and how it affects the beneficial soil microbiota in agricultural edaphic systems.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Tigecycline-Resistant Pseudomonas aeruginosa Harbouring tmexC6D6-toprJ1b From Hospital Sewage in Japan 日本医院污水中出现含tmx6d6 - toprj1b的耐替加环素铜绿假单胞菌

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2026-03-03 DOI: 10.1111/1758-2229.70275

Shotaro Maehana, Masato Suzuki, Naoko Ishimura, Hiroki Izawa, Ryotaro Eda, Masaki Nakamura, Mohan Amarasiri, Takashi Furukawa, Fumiaki Kojima, Kazunari Sei, Makoto Kubo

{"title":"Emergence of Tigecycline-Resistant Pseudomonas aeruginosa Harbouring tmexC6D6-toprJ1b From Hospital Sewage in Japan","authors":"Shotaro Maehana, Masato Suzuki, Naoko Ishimura, Hiroki Izawa, Ryotaro Eda, Masaki Nakamura, Mohan Amarasiri, Takashi Furukawa, Fumiaki Kojima, Kazunari Sei, Makoto Kubo","doi":"10.1111/1758-2229.70275","DOIUrl":"10.1111/1758-2229.70275","url":null,"abstract":"The mobile tmexCD-toprJ gene clusters encode resistance-nodulation-division (RND)-type multidrug efflux pumps which confer resistance to multiple antimicrobials, including tigecycline. Here we report the first identification of tmexCD-toprJ-harbouring Pseudomonas aeruginosa strain KAM950, isolated from hospital sewage in Japan in 2022. The isolate exhibited reduced susceptibility to tigecycline and carbapenems. Complete genome sequence analysis showed that KAM950 belongs to sequence type 244 (ST244) according to multilocus sequence typing, an internationally recognised epidemic clone, and harbours multiple antimicrobial resistance genes, including the tmexCD-toprJ variant, tmexC6D6-toprJ1b. Notably, the tmexC6D6-toprJ1b gene cluster was located on the chromosome, adjacent to the transcriptional regulator gene tnfxB6 and an IS5/IS1182 family transposase gene. Furthermore, an IS4-mediated disruption of the porin gene oprD was observed, potentially contributing to carbapenem resistance. BLASTn analysis revealed that the IS5/IS1182-tnfxB6-tmexC6D6-toprJ1b gene cluster present in both chromosomal and plasmid sequences among the order Pseudomonadaceae, indicating potential horizontal gene transfer of tnfxB6-tmexC6D6-toprJ1b mediated by IS5/IS1182. Our findings highlight the ongoing expansion of variant diversity and geographic spread of tmexCD-toprJ-like gene clusters, and underscore the importance of genomic surveillance for emerging antimicrobial resistance determinants in both clinical and environmental settings.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12956545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0