Environmental Microbiology Reports最新文献

{"title":"The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa","authors":"Dimitra Panagiotopoulou,&nbsp;Natalia Romo Catalán,&nbsp;Max Wilcox,&nbsp;Nigel Halliday,&nbsp;Paolo Pantalone,&nbsp;James Lazenby,&nbsp;Miguel Cámara,&nbsp;Stephan Heeb","doi":"10.1111/1758-2229.70090","DOIUrl":"https://doi.org/10.1111/1758-2229.70090","url":null,"abstract":"<p>Iron is an essential nutrient for microbial growth. The opportunistic pathogen <i>Pseudomonas aeruginosa</i> can survive under diverse conditions, including iron-depleted environments with the aid of small non-coding RNAs (sRNAs). <i>P. aeruginosa</i> also uses three quorum sensing (QS) systems: Las, Rhl and Pqs, to coordinate virulence and infection establishment at the population level. The aim of this study is to investigate the role of the sRNA Lrs1, the gene of which is positioned within the promoter of the Pqs biosynthetic operon <i>pqsABCDE.</i> Transcriptomics and phenotypic assays indicate that Lrs1 downregulates the production of the siderophore pyochelin but not pyoverdine, and that <i>lrs1</i> regulation itself is dependent on iron availability. Although Lrs1 has been implicated in a positive feedback loop with the transcriptional regulator LasR in the strain PA14, the present findings indicate that this is not the case in PAO1-L in the tested conditions. Transcription of Lrs1 is dependent on quorum sensing, predominantly on RhlR with an auxiliary effect by PqsE. Furthermore, the Pqs system and phenazine production are modulated by Lrs1 only under iron limitation. This study identifies Lrs1 as a new QS-dependent post-transcriptional regulator in low iron, highlighting its importance in environmental adaptation in <i>P. aeruginosa</i>.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa","authors":"Dimitra Panagiotopoulou,&nbsp;Natalia Romo Catalán,&nbsp;Max Wilcox,&nbsp;Nigel Halliday,&nbsp;Paolo Pantalone,&nbsp;James Lazenby,&nbsp;Miguel Cámara,&nbsp;Stephan Heeb","doi":"10.1111/1758-2229.70090","DOIUrl":"https://doi.org/10.1111/1758-2229.70090","url":null,"abstract":"<p>Iron is an essential nutrient for microbial growth. The opportunistic pathogen <i>Pseudomonas aeruginosa</i> can survive under diverse conditions, including iron-depleted environments with the aid of small non-coding RNAs (sRNAs). <i>P. aeruginosa</i> also uses three quorum sensing (QS) systems: Las, Rhl and Pqs, to coordinate virulence and infection establishment at the population level. The aim of this study is to investigate the role of the sRNA Lrs1, the gene of which is positioned within the promoter of the Pqs biosynthetic operon <i>pqsABCDE.</i> Transcriptomics and phenotypic assays indicate that Lrs1 downregulates the production of the siderophore pyochelin but not pyoverdine, and that <i>lrs1</i> regulation itself is dependent on iron availability. Although Lrs1 has been implicated in a positive feedback loop with the transcriptional regulator LasR in the strain PA14, the present findings indicate that this is not the case in PAO1-L in the tested conditions. Transcription of Lrs1 is dependent on quorum sensing, predominantly on RhlR with an auxiliary effect by PqsE. Furthermore, the Pqs system and phenazine production are modulated by Lrs1 only under iron limitation. This study identifies Lrs1 as a new QS-dependent post-transcriptional regulator in low iron, highlighting its importance in environmental adaptation in <i>P. aeruginosa</i>.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome Notes—Building a Library of Descriptive Microbial Ecology","authors":"Gareth Jenkins,&nbsp;Eric S. Boyd,&nbsp;Antoine Danchin,&nbsp;Vincent Hervé","doi":"10.1111/1758-2229.70085","DOIUrl":"https://doi.org/10.1111/1758-2229.70085","url":null,"abstract":"&lt;p&gt;&lt;i&gt;Environmental Microbiology Reports&lt;/i&gt; (EMIR), along with its sister journal &lt;i&gt;Environmental Microbiology&lt;/i&gt; (EMI), seek to publish research that advances knowledge on microorganisms, their function, and their interactions with the environment. The key differentiator from its sister journal, however, is that EMIR does not aim to let the subjective opinions of either editors or reviewers influence a decision on whether to publish based on the potential impact or novelty of that work. Instead, EMIR forms assessments purely based on the rigour of the methodology and the robustness of the results—an assessment of the importance of any conclusions or inferences is left to the readers.&lt;/p&gt;&lt;p&gt;This approach lets EMIR take a flexible view on what ‘should’ or ‘should not’ be published and aims to work with authors to ensure valuable work is as widely read as possible. Crucially, this means EMIR can address gaps in what is published and disseminated without making a call on whether it will impact journal metrics. Impact comes in many forms. Editors at EMIR look at what authors want to publish and position the journal as a venue to help them get that work noticed and widely disseminated.&lt;/p&gt;&lt;p&gt;With that in mind, we are launching a new article type in EMIR—&lt;b&gt;&lt;i&gt;Microbiome Notes&lt;/i&gt;&lt;/b&gt;. At both EMI and EMIR, there has been a significant increase in the number of submissions received that focus solely on microbiomes (which here we define as an examination of the microbial community present in a precisely defined environment). These papers are not necessarily hypothesis-driven but rather are descriptive and seek to enhance the body of literature around a particular taxon or environment type. Such work is consistent with a longstanding philosophy at EMIR to publish ‘short and direct science’ (Ramos &lt;span&gt;2009&lt;/span&gt;). We recognise that such descriptive papers can have tremendous value to the field; building a library of microbiome work can inform and enhance more far-reaching research by providing significantly greater context to organismal and community biodiversity.&lt;/p&gt;&lt;p&gt;Authors of descriptive microbiome work that is submitted to EMI will be encouraged to transfer their work to EMIR, consistent with the differing but complementary approaches taken at the two journals (refer author guidelines at both titles), and assessed purely on the rigour of the methodology and the robustness of the results. While not required, authors are encouraged to include as much contextual data associated with the studied microbiome, and these data need to be well-curated, machine readable and following FAIR (findable, accessible, interoperable and reusable) principles (Hertz and McNeill &lt;span&gt;2024&lt;/span&gt;). If the editors believe that the microbial and, where appropriate, contextual data reported are sound and are an advancement in the field, the journal will consider it.&lt;/p&gt;&lt;p&gt;Additionally, recognising that descriptive work is often hard to align with rigid article formatt","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome Notes—Building a Library of Descriptive Microbial Ecology","authors":"Gareth Jenkins,&nbsp;Eric S. Boyd,&nbsp;Antoine Danchin,&nbsp;Vincent Hervé","doi":"10.1111/1758-2229.70085","DOIUrl":"https://doi.org/10.1111/1758-2229.70085","url":null,"abstract":"&lt;p&gt;&lt;i&gt;Environmental Microbiology Reports&lt;/i&gt; (EMIR), along with its sister journal &lt;i&gt;Environmental Microbiology&lt;/i&gt; (EMI), seek to publish research that advances knowledge on microorganisms, their function, and their interactions with the environment. The key differentiator from its sister journal, however, is that EMIR does not aim to let the subjective opinions of either editors or reviewers influence a decision on whether to publish based on the potential impact or novelty of that work. Instead, EMIR forms assessments purely based on the rigour of the methodology and the robustness of the results—an assessment of the importance of any conclusions or inferences is left to the readers.&lt;/p&gt;&lt;p&gt;This approach lets EMIR take a flexible view on what ‘should’ or ‘should not’ be published and aims to work with authors to ensure valuable work is as widely read as possible. Crucially, this means EMIR can address gaps in what is published and disseminated without making a call on whether it will impact journal metrics. Impact comes in many forms. Editors at EMIR look at what authors want to publish and position the journal as a venue to help them get that work noticed and widely disseminated.&lt;/p&gt;&lt;p&gt;With that in mind, we are launching a new article type in EMIR—&lt;b&gt;&lt;i&gt;Microbiome Notes&lt;/i&gt;&lt;/b&gt;. At both EMI and EMIR, there has been a significant increase in the number of submissions received that focus solely on microbiomes (which here we define as an examination of the microbial community present in a precisely defined environment). These papers are not necessarily hypothesis-driven but rather are descriptive and seek to enhance the body of literature around a particular taxon or environment type. Such work is consistent with a longstanding philosophy at EMIR to publish ‘short and direct science’ (Ramos &lt;span&gt;2009&lt;/span&gt;). We recognise that such descriptive papers can have tremendous value to the field; building a library of microbiome work can inform and enhance more far-reaching research by providing significantly greater context to organismal and community biodiversity.&lt;/p&gt;&lt;p&gt;Authors of descriptive microbiome work that is submitted to EMI will be encouraged to transfer their work to EMIR, consistent with the differing but complementary approaches taken at the two journals (refer author guidelines at both titles), and assessed purely on the rigour of the methodology and the robustness of the results. While not required, authors are encouraged to include as much contextual data associated with the studied microbiome, and these data need to be well-curated, machine readable and following FAIR (findable, accessible, interoperable and reusable) principles (Hertz and McNeill &lt;span&gt;2024&lt;/span&gt;). If the editors believe that the microbial and, where appropriate, contextual data reported are sound and are an advancement in the field, the journal will consider it.&lt;/p&gt;&lt;p&gt;Additionally, recognising that descriptive work is often hard to align with rigid article formatt","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Insights Into the Biological and Physical–Chemical Diversity of Various Salt Ponds of Trapani, Sicily","authors":"Benvenuta Sonia Cusenza,&nbsp;Giuseppe Scelfo,&nbsp;Gabriella Licata,&nbsp;Fanny Claire Capri,&nbsp;Fabrizio Vicari,&nbsp;Rosa Alduina,&nbsp;Valeria Villanova","doi":"10.1111/1758-2229.70075","DOIUrl":"10.1111/1758-2229.70075","url":null,"abstract":"<p>The salt ponds of Trapani, Sicily, represent an extreme and under-explored ecosystem characterised by varying salinity gradients and environmental conditions. These ponds, integral to traditional salt extraction, include cold, driving, hot and crystallizer ponds, each hosting diverse microbial communities. This study aimed to explore the biological and physical–chemical diversity of 11 ponds during the salt production season in Trapani. We conducted comprehensive physical–chemical characterizations, including measurements of pH, conductivity, viscosity, density, organic carbon and ion concentration. Microbial DNA was extracted from salt pond waters and subjected to metabarcoding of 16S rRNA genes to determine the diversity of archaea and bacteria. High-throughput sequencing revealed significant variations in microbial communities across different pond types and seasons. Cold ponds showed a higher diversity of moderately halophilic organisms, while crystallizer and feeding ponds were dominated by extreme halophiles, particularly archaeal genus <i>Halorubrum</i> and <i>Haloquadratum</i> and bacterial genus <i>Salinibacter</i>. Statistical analyses indicated that environmental parameters, especially salinity and temperature, significantly influenced microbial community composition. Our findings enhance the understanding of microbial ecology in saline environments and highlight the potential of halophilic microorganisms. This study provides a foundation for future research into the functional roles of these microorganisms and their industrial applications.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and Depth-Driven Variability of Pelagic Bacterial Communities in Lake Erie: Biofilm and Plankton Dynamics","authors":"Rylie L. Robinson,&nbsp;Aaron T. Fisk,&nbsp;Sophie Crevecoeur","doi":"10.1111/1758-2229.70079","DOIUrl":"10.1111/1758-2229.70079","url":null,"abstract":"<p>Despite constituting an important component of freshwater ecosystems, biofilm assemblages have remained relatively understudied compared to plankton, especially in freshwater systems such as the western basin of Lake Erie (WBLE). This study therefore aimed to elucidate temporal and vertical shifts of microbial communities of planktonic and biofilm growth on artificial substrates in the WBLE water column at discrete depths, investigating the overlap of shared taxa between community types. Sequencing of the 16S rRNA gene revealed concurrent biofilm-plankton samples shared a low percentage (~10%) of amplicon sequence variants (ASVs) indicating distinct communities between free-living and substrate-attached bacteria. Plankton communities did not significantly differ between surface and bottom depths (1 and 8 m), whereas biofilm communities differed between upper (1–4 m) and lower (5–8 m) water columns. Temporal variation in community composition was observed in biofilm, with early periods (June–July) showing significant dissimilarity followed by compositional convergence in late summer onwards (August–October). With the expansion of artificial infrastructure in aquatic systems, there is novel substrate material to observe spatiotemporal patterns of microbial colonisation throughout the pelagic zone. These results demonstrate the complexity of bacterial biofilm communities from plankton in freshwater, providing insight into microbial assembly through temporal succession and across depth.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Suitable, Readily Available, Sources of Sulfate-Reducing Bacteria Inoculum, and Evaluation of Sulfate Reduction Rates Achieved at Different pHs","authors":"Janet Smith,&nbsp;Craig Sheridan,&nbsp;Lizelle van Dyk,&nbsp;Kevin G. Harding","doi":"10.1111/1758-2229.70081","DOIUrl":"https://doi.org/10.1111/1758-2229.70081","url":null,"abstract":"<p>This study investigated the suitability of readily available and naturally occurring sources of microorganisms (inoculum) to use for the cultivation of sulphate-reducing bacteria (SRB) for acid mine drainage (AMD) remediation. The selected inocula included AMD water (AMD), mud (MUD) and reed-bed mud (RM) from the AMD surrounds, mealworms (MW), cow dung (CD) and raw sewage sludge (RS). The suitability of the different inoculum sources was evaluated by comparing the SO₄²⁻ reduction and sulfide (S²⁻) production rates at three different pHs. Experimental results showed that the AMD, MW, MUD and CD inoculum did not produce appreciable reduction of SO₄²⁻ to S²⁻ and were unsuitable sources of SRB inoculum. The inoculum evaluated in pH 2 media did not achieve SO₄²⁻ reduction. Of the inoculum assessed in pH 4 media, only the RM inoculum achieved SO₄²⁻ reduction (40%) with S²⁻ production (36 mg/L). In contrast, a notable S²⁻ production, RS (114 mg/L) and RM (99 mg/L), accompanied the SO₄²⁻ reduction achieved in the pH 7.5 RS (44%) and RM (30%) samples. The improved S²⁻_produced/SO₄²⁻_removed conversion ratios for samples pH 7.5 RS (0.14) and pH 7.5 RM (0.17) are indicative of increased SRB activity and the suitability of these inoculum as SRB sources.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Suitable, Readily Available, Sources of Sulfate-Reducing Bacteria Inoculum, and Evaluation of Sulfate Reduction Rates Achieved at Different pHs","authors":"Janet Smith,&nbsp;Craig Sheridan,&nbsp;Lizelle van Dyk,&nbsp;Kevin G. Harding","doi":"10.1111/1758-2229.70081","DOIUrl":"https://doi.org/10.1111/1758-2229.70081","url":null,"abstract":"<p>This study investigated the suitability of readily available and naturally occurring sources of microorganisms (inoculum) to use for the cultivation of sulphate-reducing bacteria (SRB) for acid mine drainage (AMD) remediation. The selected inocula included AMD water (AMD), mud (MUD) and reed-bed mud (RM) from the AMD surrounds, mealworms (MW), cow dung (CD) and raw sewage sludge (RS). The suitability of the different inoculum sources was evaluated by comparing the SO₄²⁻ reduction and sulfide (S²⁻) production rates at three different pHs. Experimental results showed that the AMD, MW, MUD and CD inoculum did not produce appreciable reduction of SO₄²⁻ to S²⁻ and were unsuitable sources of SRB inoculum. The inoculum evaluated in pH 2 media did not achieve SO₄²⁻ reduction. Of the inoculum assessed in pH 4 media, only the RM inoculum achieved SO₄²⁻ reduction (40%) with S²⁻ production (36 mg/L). In contrast, a notable S²⁻ production, RS (114 mg/L) and RM (99 mg/L), accompanied the SO₄²⁻ reduction achieved in the pH 7.5 RS (44%) and RM (30%) samples. The improved S²⁻_produced/SO₄²⁻_removed conversion ratios for samples pH 7.5 RS (0.14) and pH 7.5 RM (0.17) are indicative of increased SRB activity and the suitability of these inoculum as SRB sources.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Year-Round Quantification, Structure and Dynamics of Epibacterial Communities From Diverse Macroalgae Reveal a Persistent Core Microbiota and Strong Host Specificities","authors":"Maéva Brunet,&nbsp;Nolwen Le Duff,&nbsp;Tristan Barbeyron,&nbsp;François Thomas","doi":"10.1111/1758-2229.70077","DOIUrl":"10.1111/1758-2229.70077","url":null,"abstract":"<p>Macroalgae-bacteria interactions play pivotal ecological roles in coastal ecosystems. Previous characterisation of surface microbiota from various macroalgae evidenced fluctuations based on host tissues, physicochemical and environmental parameters. However, the dynamics and degree of similarity of epibacterial communities colonising phylogenetically distant algae from the same habitat are still elusive. We conducted a year-long monthly epimicrobiota sampling on five algal species inhabiting an English Channel rocky shore: <i>Laminaria digitata</i>, <i>Ascophyllum nodosum</i>, <i>Fucus serratus</i> (brown algae), <i>Palmaria palmata</i> (red alga) and <i>Ulva</i> sp. (green alga). To go beyond relative compositional data and estimate absolute variations in taxa abundance, we combined qPCR measurements of 16S rRNA gene copies with amplicon metabarcoding. A core microbiome composed of 10 genera was consistently found year-round on all algae. Notably, the abundant genus <i>Granulosicoccus</i> stood out for being the only one present in all samples and displayed an important microdiversity. Algal host emerged as the primary driver of epibacterial community composition, before seasonality, and bacterial taxa specifically associated with one or several algae were identified. Moreover, the impact of seasons on the epimicrobiota varied depending on algal tissues. Overall, this study provides an extensive characterisation of the microbiota of intertidal macroalgae and enhances our understanding of algal-bacteria holobionts.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Microbiological Dynamics in a Salt Cavern for Potential Hydrogen Storage Use","authors":"Nicole Dopffel,&nbsp;Kyle Mayers,&nbsp;Abduljelil Kedir,&nbsp;Biwen Annie An-Stepec,&nbsp;Janiche Beeder,&nbsp;Silvan Hoth","doi":"10.1111/1758-2229.70064","DOIUrl":"10.1111/1758-2229.70064","url":null,"abstract":"<p>Hydrogen storage in salt caverns is important for supporting the energy transition. However, there is limited knowledge about microbial communities within these caverns and associated risks of hydrogen loss. In this study we characterised a salt-saturated brine from a salt cavern and found a high sulphate content (4.2 g/L) and low carbon content (84.9 mg/L inorganic, 7.61 mg/L organic). The brine contained both Bacteria and Archaea, and 16S rRNA gene analysis revealed a halophilic community with members of <i>Acetohalobium</i>, <i>Thiohalorhabdus</i>, <i>Salinibacter</i> and up to 40% of unknown sequences. Within the Archaea, Euryarchaeota and the symbiotic Nanohaloarcheaota were dominant. Growth experiments showed that some microbes are resistant to autoclaving and pass through 0.22 μm filters. <i>Heyndrickxia</i>-related colonies grew on aerobic plates up to 10% salt, indicating the presence of inactive spores. The highest anaerobic activity was observed at 30°C, including glucose- and yeast extract fermentation, hydrogen-oxidation, lactate-utilisation, methane- and acetate-formation and sulphate-reduction, which was observed up to 80°C. However, microbial activity was slow, with incubations taking up to 1 year to measure microbial products. This study indicates that artificial salt caverns are an extreme environment containing potential hydrogen-consuming microbes.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}