Environmental Microbiology Reports最新文献_第4页

Metabolomic and Genomic Analysis of Bioactive Compounds of Phacidium infestans Karsten DSM 5139 Cultivated on Pinus sylvestris Needles 在西林松针上培养的鼠疫杆菌（Phacidium infestans Karsten DSM 5139）生物活性物质的代谢组学和基因组学分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-09 DOI: 10.1111/1758-2229.70084

Chahira Zerouki, Omolara Mofikoya, Taskeen Badar, Marko Mäkinen, Ossi Turunen, Janne Jänis

{"title":"Metabolomic and Genomic Analysis of Bioactive Compounds of Phacidium infestans Karsten DSM 5139 Cultivated on Pinus sylvestris Needles","authors":"Chahira Zerouki, Omolara Mofikoya, Taskeen Badar, Marko Mäkinen, Ossi Turunen, Janne Jänis","doi":"10.1111/1758-2229.70084","DOIUrl":"https://doi.org/10.1111/1758-2229.70084","url":null,"abstract":"This study investigates how Phacidium infestans acquires nutrients on Pinus sylvestris needles, which possess antimicrobial properties. P. infestans was evaluated for its growth and enzyme production on various substrates, alongside genomic and metabolomic analysis. Direct-infusion high-resolution mass spectrometry (DI-HRMS) was performed on methanol extracts obtained from P. infestans cultivated on needles and malt extract media. DI-HRMS analysis identified 21 compounds from the malt extract and 112 from the needle samples. The resin components increased in the needle samples post-cultivation, suggesting terpenoid release from resin ducts due to fungal degradation of plant cell walls. P. infestans fully consumed sugars and antifungal compounds, including taxiresinol and salicylic acid, with control-to-sample ratios (CTR/SA) of 289.76 and 47.24, respectively. Moreover, lariciresinol and pinoresinol were reduced to undetectable levels. The genomic analysis identified 421 secreted proteins, including 128 carbohydrate-active enzymes, 3 cutinases, and 49 lipases that aid host penetration and wax degradation. Several multi-drug efflux pumps and two acyclic terpene utilisation proteins were identified as well. These proteins support the cellular integrity of P. infestans by expelling toxic compounds. Our findings provide valuable insights into the metabolic strategies of P. infestans for nutrient assimilation on pine needles.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic and Genomic Analysis of Bioactive Compounds of Phacidium infestans Karsten DSM 5139 Cultivated on Pinus sylvestris Needles 在西林松针上培养的鼠疫杆菌（Phacidium infestans Karsten DSM 5139）生物活性物质的代谢组学和基因组学分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-09 DOI: 10.1111/1758-2229.70084

Chahira Zerouki, Omolara Mofikoya, Taskeen Badar, Marko Mäkinen, Ossi Turunen, Janne Jänis

{"title":"Metabolomic and Genomic Analysis of Bioactive Compounds of Phacidium infestans Karsten DSM 5139 Cultivated on Pinus sylvestris Needles","authors":"Chahira Zerouki, Omolara Mofikoya, Taskeen Badar, Marko Mäkinen, Ossi Turunen, Janne Jänis","doi":"10.1111/1758-2229.70084","DOIUrl":"https://doi.org/10.1111/1758-2229.70084","url":null,"abstract":"This study investigates how Phacidium infestans acquires nutrients on Pinus sylvestris needles, which possess antimicrobial properties. P. infestans was evaluated for its growth and enzyme production on various substrates, alongside genomic and metabolomic analysis. Direct-infusion high-resolution mass spectrometry (DI-HRMS) was performed on methanol extracts obtained from P. infestans cultivated on needles and malt extract media. DI-HRMS analysis identified 21 compounds from the malt extract and 112 from the needle samples. The resin components increased in the needle samples post-cultivation, suggesting terpenoid release from resin ducts due to fungal degradation of plant cell walls. P. infestans fully consumed sugars and antifungal compounds, including taxiresinol and salicylic acid, with control-to-sample ratios (CTR/SA) of 289.76 and 47.24, respectively. Moreover, lariciresinol and pinoresinol were reduced to undetectable levels. The genomic analysis identified 421 secreted proteins, including 128 carbohydrate-active enzymes, 3 cutinases, and 49 lipases that aid host penetration and wax degradation. Several multi-drug efflux pumps and two acyclic terpene utilisation proteins were identified as well. These proteins support the cellular integrity of P. infestans by expelling toxic compounds. Our findings provide valuable insights into the metabolic strategies of P. infestans for nutrient assimilation on pine needles.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characterisation of the Bacterial Microbiota Associating With a Strain of Epichloë Fungal Endophyte of Perennial Ryegrass and the Interaction With Its Paenibacillus Members 多年生黑麦草Epichloë真菌内生菌相关菌群的遗传特征及其与芽孢杆菌成员的相互作用

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-08 DOI: 10.1111/1758-2229.70113

Daniel A. Bastías, Linda J. Johnson, Sandeep Kumar, Ruy Jáuregui, Emma R. Applegate, Stuart D. Card

{"title":"Genetic Characterisation of the Bacterial Microbiota Associating With a Strain of Epichloë Fungal Endophyte of Perennial Ryegrass and the Interaction With Its Paenibacillus Members","authors":"Daniel A. Bastías, Linda J. Johnson, Sandeep Kumar, Ruy Jáuregui, Emma R. Applegate, Stuart D. Card","doi":"10.1111/1758-2229.70113","DOIUrl":"https://doi.org/10.1111/1758-2229.70113","url":null,"abstract":"Plant-associated fungi can host unique bacterial microbiota to provide multiple benefits to their fungal hosts. Here it was characterised the bacterial microbiota associated with an Epichloë fungal endophyte (strain AR135) isolated from perennial ryegrass (Lolium perenne) via both 16S rRNA gene sequencing and direct microbial isolation and investigated the microbe-microbe interactions between these bacteria and the fungus. The bacterial microbiota of AR135 was dominated by members within the genus Paenibacillus, with 99% of abundance (on average); although bacteria within genera Delftia and Bradyrhizobium were also present. Paenibacillus cells were located on the surface of hyphae of AR135 fungus in vitro on synthetic media and in planta within perennial ryegrass leaves. Two bacterial strains, E100 and E300, identified as Paenibacillus, were isolated from the AR135 mycelium. E300 drastically altered the abundance of both the whole bacterial microbiota (increased by 63%) and E100 (reduced to 0%). None of the variations observed in the abundance of total bacterial microbiota and E100 and E300 were associated with changes in the fungal biomass of Epichloë. The findings show that Epichloë fungal endophytes can host bacterial communities, the structure of which was regulated by key members of the bacterial community.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characterisation of the Bacterial Microbiota Associating With a Strain of Epichloë Fungal Endophyte of Perennial Ryegrass and the Interaction With Its Paenibacillus Members 多年生黑麦草Epichloë真菌内生菌相关菌群的遗传特征及其与芽孢杆菌成员的相互作用

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-08 DOI: 10.1111/1758-2229.70113

Daniel A. Bastías, Linda J. Johnson, Sandeep Kumar, Ruy Jáuregui, Emma R. Applegate, Stuart D. Card

{"title":"Genetic Characterisation of the Bacterial Microbiota Associating With a Strain of Epichloë Fungal Endophyte of Perennial Ryegrass and the Interaction With Its Paenibacillus Members","authors":"Daniel A. Bastías, Linda J. Johnson, Sandeep Kumar, Ruy Jáuregui, Emma R. Applegate, Stuart D. Card","doi":"10.1111/1758-2229.70113","DOIUrl":"https://doi.org/10.1111/1758-2229.70113","url":null,"abstract":"Plant-associated fungi can host unique bacterial microbiota to provide multiple benefits to their fungal hosts. Here it was characterised the bacterial microbiota associated with an Epichloë fungal endophyte (strain AR135) isolated from perennial ryegrass (Lolium perenne) via both 16S rRNA gene sequencing and direct microbial isolation and investigated the microbe-microbe interactions between these bacteria and the fungus. The bacterial microbiota of AR135 was dominated by members within the genus Paenibacillus, with 99% of abundance (on average); although bacteria within genera Delftia and Bradyrhizobium were also present. Paenibacillus cells were located on the surface of hyphae of AR135 fungus in vitro on synthetic media and in planta within perennial ryegrass leaves. Two bacterial strains, E100 and E300, identified as Paenibacillus, were isolated from the AR135 mycelium. E300 drastically altered the abundance of both the whole bacterial microbiota (increased by 63%) and E100 (reduced to 0%). None of the variations observed in the abundance of total bacterial microbiota and E100 and E300 were associated with changes in the fungal biomass of Epichloë. The findings show that Epichloë fungal endophytes can host bacterial communities, the structure of which was regulated by key members of the bacterial community.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems 不同地下生态系统中候选念珠菌核心代谢功能的差异表达

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-03 DOI: 10.1111/1758-2229.70096

Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst

{"title":"Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems","authors":"Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst","doi":"10.1111/1758-2229.70096","DOIUrl":"https://doi.org/10.1111/1758-2229.70096","url":null,"abstract":"Candidatus Altiarchaea are widespread across aquatic subsurface ecosystems and possess a highly conserved core genome, yet adaptations of this core genome to different biotic and abiotic factors based on gene expression remain unknown. Here, we investigated the metatranscriptome of two Ca. Altiarchaeum populations that thrive in two substantially different subsurface ecosystems. In Crystal Geyser, a high-CO2 groundwater system in the USA, Ca. Altiarchaeum crystalense co-occurs with the symbiont Ca. Huberiarchaeum crystalense, while in the Muehlbacher sulfidic spring in Germany, an artesian spring high in sulfide concentration, Ca. A. hamiconexum is heavily infected with viruses. We here mapped metatranscriptome reads against their genomes to analyse the in situ expression profile of their core genomes. Out of 537 shared gene clusters, 331 were functionally annotated and 130 differed significantly in expression between the two sites. Main differences were related to genes involved in cell defence like CRISPR-Cas, virus defence, replication, transcription and energy and carbon metabolism. Our results demonstrate that altiarchaeal populations in the subsurface are likely adapted to their environment while influenced by other biological entities that tamper with their core metabolism. We consequently posit that viruses and symbiotic interactions can be major energy sinks for organisms in the deep biosphere.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems 不同地下生态系统中候选念珠菌核心代谢功能的差异表达

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-03 DOI: 10.1111/1758-2229.70096

Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst

{"title":"Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems","authors":"Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst","doi":"10.1111/1758-2229.70096","DOIUrl":"https://doi.org/10.1111/1758-2229.70096","url":null,"abstract":"Candidatus Altiarchaea are widespread across aquatic subsurface ecosystems and possess a highly conserved core genome, yet adaptations of this core genome to different biotic and abiotic factors based on gene expression remain unknown. Here, we investigated the metatranscriptome of two Ca. Altiarchaeum populations that thrive in two substantially different subsurface ecosystems. In Crystal Geyser, a high-CO2 groundwater system in the USA, Ca. Altiarchaeum crystalense co-occurs with the symbiont Ca. Huberiarchaeum crystalense, while in the Muehlbacher sulfidic spring in Germany, an artesian spring high in sulfide concentration, Ca. A. hamiconexum is heavily infected with viruses. We here mapped metatranscriptome reads against their genomes to analyse the in situ expression profile of their core genomes. Out of 537 shared gene clusters, 331 were functionally annotated and 130 differed significantly in expression between the two sites. Main differences were related to genes involved in cell defence like CRISPR-Cas, virus defence, replication, transcription and energy and carbon metabolism. Our results demonstrate that altiarchaeal populations in the subsurface are likely adapted to their environment while influenced by other biological entities that tamper with their core metabolism. We consequently posit that viruses and symbiotic interactions can be major energy sinks for organisms in the deep biosphere.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation 铁限制下硼库木香SK2基因表达、蛋白质合成及表观遗传修饰的综合分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-02 DOI: 10.1111/1758-2229.70106

Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva

{"title":"Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation","authors":"Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva","doi":"10.1111/1758-2229.70106","DOIUrl":"https://doi.org/10.1111/1758-2229.70106","url":null,"abstract":"This study aimed to understand the genetic and molecular mechanisms enabling Alcanivorax borkumensis SK2, a hydrocarbonoclastic marine bacterium, to thrive under iron-limited conditions. Using SMRT PacBio whole-genome sequencing, Illumina total RNA sequencing, and proteomics analysis, we examined the strain's response to iron-rich and iron-depleted media. Despite minimal impact on growth, significant changes in gene expression were observed when using n-tetradecane or acetate under iron limitation. Iron scarcity, depending on the carbon source, affects energy metabolism, membrane transport, lipid metabolism, stress-adaptive responses, and siderophore synthesis. We identified several methyltransferases (MTases) in the studied genome, including RS14230, which is a part of a fully functional restriction-modification (RM) system causing bipartite cytosine methylation and DNA cleavage at AgGCcT sites. Another MTase, RS09425, controls bipartite adenine methylation at GaTNNNNNGtGG motifs; however, no restriction activity at these motifs has been detected. Many epigenetically modified nucleotides lacked canonical motifs, possibly due to MTase byproducts. Notably, non-canonical modifications were statistically associated with transcriptional start sites and gene regulation, suggesting an indirect role in transcription via DNA conformation changes and its accessibility to MTases near actively transcribed genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation 铁限制下硼库木香SK2基因表达、蛋白质合成及表观遗传修饰的综合分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-02 DOI: 10.1111/1758-2229.70106

Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva

{"title":"Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation","authors":"Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva","doi":"10.1111/1758-2229.70106","DOIUrl":"https://doi.org/10.1111/1758-2229.70106","url":null,"abstract":"This study aimed to understand the genetic and molecular mechanisms enabling Alcanivorax borkumensis SK2, a hydrocarbonoclastic marine bacterium, to thrive under iron-limited conditions. Using SMRT PacBio whole-genome sequencing, Illumina total RNA sequencing, and proteomics analysis, we examined the strain's response to iron-rich and iron-depleted media. Despite minimal impact on growth, significant changes in gene expression were observed when using n-tetradecane or acetate under iron limitation. Iron scarcity, depending on the carbon source, affects energy metabolism, membrane transport, lipid metabolism, stress-adaptive responses, and siderophore synthesis. We identified several methyltransferases (MTases) in the studied genome, including RS14230, which is a part of a fully functional restriction-modification (RM) system causing bipartite cytosine methylation and DNA cleavage at AgGCcT sites. Another MTase, RS09425, controls bipartite adenine methylation at GaTNNNNNGtGG motifs; however, no restriction activity at these motifs has been detected. Many epigenetically modified nucleotides lacked canonical motifs, possibly due to MTase byproducts. Notably, non-canonical modifications were statistically associated with transcriptional start sites and gene regulation, suggesting an indirect role in transcription via DNA conformation changes and its accessibility to MTases near actively transcribed genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms 生物可利用的溶解有机碳是河流生物膜中磷动态的关键调节因子

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-01 DOI: 10.1111/1758-2229.70115

Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere

{"title":"Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms","authors":"Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere","doi":"10.1111/1758-2229.70115","DOIUrl":"https://doi.org/10.1111/1758-2229.70115","url":null,"abstract":"Phosphorus (P) dynamics at the sediment–water interface of aquatic ecosystems are receiving increasing attention due to their implications for water quality. P uptake by microbial biofilms can serve as a mechanism to control and mitigate the risk of eutrophication. Microbial biofilms capture P both intracellularly and extracellularly. While the significance of extracellular P entrapment in biofilms in engineered systems has recently been established, little is known about its dynamics in aquatic ecosystems. Current research on eutrophication control predominantly emphasises nitrogen, phosphorus or nitrogen-phosphorus ratio-based approaches, often overlooking the potential indirect influence of bioavailable dissolved organic carbon (DOC) on P uptake by heterotrophic microorganisms. In this study, we tested the effect of bioavailable DOC on P entrapment patterns in biofilms and in biofilm P-regulation mechanisms such as polyphosphate accumulation and alkaline phosphatase activity in semi-natural flow-through experimental flumes. Our results show that intracellular P entrapment is limited by bioavailable DOC, while extracellular P entrapment is independent of bioavailable DOC and has the potential to offset intracellular P saturation. We further demonstrate that DOC bioavailability influences benthic P cycling and that its implications extend into critical areas of ecosystem functioning such as river self-purification, competitive resource utilisation and organic P cycling.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms 生物可利用的溶解有机碳是河流生物膜中磷动态的关键调节因子

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-01 DOI: 10.1111/1758-2229.70115

Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere

{"title":"Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms","authors":"Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere","doi":"10.1111/1758-2229.70115","DOIUrl":"https://doi.org/10.1111/1758-2229.70115","url":null,"abstract":"Phosphorus (P) dynamics at the sediment–water interface of aquatic ecosystems are receiving increasing attention due to their implications for water quality. P uptake by microbial biofilms can serve as a mechanism to control and mitigate the risk of eutrophication. Microbial biofilms capture P both intracellularly and extracellularly. While the significance of extracellular P entrapment in biofilms in engineered systems has recently been established, little is known about its dynamics in aquatic ecosystems. Current research on eutrophication control predominantly emphasises nitrogen, phosphorus or nitrogen-phosphorus ratio-based approaches, often overlooking the potential indirect influence of bioavailable dissolved organic carbon (DOC) on P uptake by heterotrophic microorganisms. In this study, we tested the effect of bioavailable DOC on P entrapment patterns in biofilms and in biofilm P-regulation mechanisms such as polyphosphate accumulation and alkaline phosphatase activity in semi-natural flow-through experimental flumes. Our results show that intracellular P entrapment is limited by bioavailable DOC, while extracellular P entrapment is independent of bioavailable DOC and has the potential to offset intracellular P saturation. We further demonstrate that DOC bioavailability influences benthic P cycling and that its implications extend into critical areas of ecosystem functioning such as river self-purification, competitive resource utilisation and organic P cycling.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0