Environmental Microbiology Reports最新文献_第5页

Forest Type, Bark Wounding, and Tapping: Their Combined Influence on Bacteria Biota of Styrax Paralleloneurus in Natural and Community Forest 森林类型、树皮损伤和攻穗对天然和群落林平行柱蕨细菌生物群的综合影响

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-04 DOI: 10.1111/1758-2229.70184

Arida Susilowati, Margaretta Christita, Siti Halimah Larekeng, Adebola Azeez Lateef, Wenzi Ren, Abiodun A. Azeez, Rumella Simarmata, Yeni Khairina, Fiqriah Hanum Khumairah, Deni Elfiati, Fred O. Asiegbu

{"title":"Forest Type, Bark Wounding, and Tapping: Their Combined Influence on Bacteria Biota of Styrax Paralleloneurus in Natural and Community Forest","authors":"Arida Susilowati, Margaretta Christita, Siti Halimah Larekeng, Adebola Azeez Lateef, Wenzi Ren, Abiodun A. Azeez, Rumella Simarmata, Yeni Khairina, Fiqriah Hanum Khumairah, Deni Elfiati, Fred O. Asiegbu","doi":"10.1111/1758-2229.70184","DOIUrl":"10.1111/1758-2229.70184","url":null,"abstract":"Styrax paralleloneurus is a resin-producing tree native to Sumatra, Indonesia. This study investigated the effects of tapping, bark wounding and forest type on bacterial biota in the stem of styrax in natural and community forests. Amplicon metagenomic sequencing of the 16S rRNA region was deployed to identify the bacterial communities associated with tapped and untapped trees across various environmental and experimental conditions. The results of the study showed that tapped trees had lower abundance and diversity of Pseudomonas compared to untapped trees, largely due to their increased exposure to external microbe communities and environmental elements. Serratia and Pantoea were more abundant in natural forest than community forest, while Bradyrhizobium lablabi was found abundantly in untapped trees. Additionally, the taxonomic analysis revealed distinct responses of bacterial genera to tapping and forest type, indicating that community forests could play a significant role in promoting biodiversity in forest ecosystems. This finding underscores the importance of community forests in biodiversity conservation. These insights can inform future conservation and management strategies to enhance biodiversity and underscore the need for sustainable forest management practices to maintain forest health and productivity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Composition in a Common Mediterranean Bryozoan Following an Unprecedented Marine Heatwave 在前所未有的海洋热浪之后，普通地中海苔藓虫的微生物组成

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-04 DOI: 10.1111/1758-2229.70185

Blanca Figuerola, Cristina Linares, Claudia Aparicio-Estalella, Paula López-Sendino, Joaquim Garrabou, Javier del Campo

{"title":"Microbiome Composition in a Common Mediterranean Bryozoan Following an Unprecedented Marine Heatwave","authors":"Blanca Figuerola, Cristina Linares, Claudia Aparicio-Estalella, Paula López-Sendino, Joaquim Garrabou, Javier del Campo","doi":"10.1111/1758-2229.70185","DOIUrl":"10.1111/1758-2229.70185","url":null,"abstract":"Marine heatwaves are intensifying due to global warming and increasingly drive mass mortality events in shallow benthic ecosystems. Marine invertebrates host diverse microbial communities that contribute to their health and resilience, yet microbiome responses under thermal stress remain poorly characterised across most taxa. Here, we characterise the microbiome composition in colonies of the common Mediterranean bryozoan Myriapora truncata at two depths (13 and 17 m) following the extreme 2022 marine heatwave. Despite no visible necrosis, microbial communities at both depths exhibited shifts indicative of thermal stress, including the reduced presence of potential core microbial members. Colonies from the shallower, warmer depth showed higher alpha diversity and reduced abundance of key functional genera compared to deeper colonies, suggesting early dysbiosis. These results highlight that M. truncata—though visually unaffected—undergoes sublethal microbiome alterations under thermal stress. This study provides the first characterisation of a bryozoan microbiome after a marine heatwave and highlights the potential of host-associated microbial communities as early bioindicators of invertebrate stress in a warming ocean.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forest Type, Bark Wounding, and Tapping: Their Combined Influence on Bacteria Biota of Styrax Paralleloneurus in Natural and Community Forest 森林类型、树皮损伤和攻穗对天然和群落林平行柱蕨细菌生物群的综合影响

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-04 DOI: 10.1111/1758-2229.70184

Arida Susilowati, Margaretta Christita, Siti Halimah Larekeng, Adebola Azeez Lateef, Wenzi Ren, Abiodun A. Azeez, Rumella Simarmata, Yeni Khairina, Fiqriah Hanum Khumairah, Deni Elfiati, Fred O. Asiegbu

{"title":"Forest Type, Bark Wounding, and Tapping: Their Combined Influence on Bacteria Biota of Styrax Paralleloneurus in Natural and Community Forest","authors":"Arida Susilowati, Margaretta Christita, Siti Halimah Larekeng, Adebola Azeez Lateef, Wenzi Ren, Abiodun A. Azeez, Rumella Simarmata, Yeni Khairina, Fiqriah Hanum Khumairah, Deni Elfiati, Fred O. Asiegbu","doi":"10.1111/1758-2229.70184","DOIUrl":"10.1111/1758-2229.70184","url":null,"abstract":"Styrax paralleloneurus is a resin-producing tree native to Sumatra, Indonesia. This study investigated the effects of tapping, bark wounding and forest type on bacterial biota in the stem of styrax in natural and community forests. Amplicon metagenomic sequencing of the 16S rRNA region was deployed to identify the bacterial communities associated with tapped and untapped trees across various environmental and experimental conditions. The results of the study showed that tapped trees had lower abundance and diversity of Pseudomonas compared to untapped trees, largely due to their increased exposure to external microbe communities and environmental elements. Serratia and Pantoea were more abundant in natural forest than community forest, while Bradyrhizobium lablabi was found abundantly in untapped trees. Additionally, the taxonomic analysis revealed distinct responses of bacterial genera to tapping and forest type, indicating that community forests could play a significant role in promoting biodiversity in forest ecosystems. This finding underscores the importance of community forests in biodiversity conservation. These insights can inform future conservation and management strategies to enhance biodiversity and underscore the need for sustainable forest management practices to maintain forest health and productivity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Substrate Impacts Microcystis aeruginosa Exometabolome Composition 氮底物对铜绿微囊藻外代谢组组成的影响

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-02 DOI: 10.1111/1758-2229.70189

Caroline M. Peck, Lauren N. Hart, Roland Kersten, Jenan J. Kharbush

{"title":"Nitrogen Substrate Impacts Microcystis aeruginosa Exometabolome Composition","authors":"Caroline M. Peck, Lauren N. Hart, Roland Kersten, Jenan J. Kharbush","doi":"10.1111/1758-2229.70189","DOIUrl":"10.1111/1758-2229.70189","url":null,"abstract":"Microcystis aeruginosa is a toxic cyanobacteria species that is often abundant during cyanobacterial harmful algal blooms (cyanoHABs) in freshwaters. This study examined how growth on different nitrogen substrates influences the exometabolome of toxic and non-toxic strains of M. aeruginosa. We used untargeted metabolomics, with liquid chromatography-mass spectrometry of metabolites followed by feature-based molecular networking and in silico metabolite annotation. Molecules released by M. aeruginosa varied based on the type of N substrate provided: the exometabolomes of cultures grown on ammonium and urea were more similar to each other and distinct from those grown on nitrate, suggesting that different assimilatory energetic requirements between reduced and oxidised N substrates are an important driver of exometabolome composition. Amino acids and peptides were the dominant compound class among metabolites that were significantly different between N treatments, but responses to N substrate were also reflected in altered extracellular concentrations of lipids, cyanotoxins, and photoprotectants. These differences in the molecular-level response to the type of N substrate supplied support that environmental factors like changing N availability and oxidative stress may synergistically influence M. aeruginosa strain fitness and community succession, as well as interactions between M. aeruginosa strains and other bacteria or cyanobacteria in the bloom community.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Substrate Impacts Microcystis aeruginosa Exometabolome Composition 氮底物对铜绿微囊藻外代谢组组成的影响

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-02 DOI: 10.1111/1758-2229.70189

Caroline M. Peck, Lauren N. Hart, Roland Kersten, Jenan J. Kharbush

{"title":"Nitrogen Substrate Impacts Microcystis aeruginosa Exometabolome Composition","authors":"Caroline M. Peck, Lauren N. Hart, Roland Kersten, Jenan J. Kharbush","doi":"10.1111/1758-2229.70189","DOIUrl":"10.1111/1758-2229.70189","url":null,"abstract":"Microcystis aeruginosa is a toxic cyanobacteria species that is often abundant during cyanobacterial harmful algal blooms (cyanoHABs) in freshwaters. This study examined how growth on different nitrogen substrates influences the exometabolome of toxic and non-toxic strains of M. aeruginosa. We used untargeted metabolomics, with liquid chromatography-mass spectrometry of metabolites followed by feature-based molecular networking and in silico metabolite annotation. Molecules released by M. aeruginosa varied based on the type of N substrate provided: the exometabolomes of cultures grown on ammonium and urea were more similar to each other and distinct from those grown on nitrate, suggesting that different assimilatory energetic requirements between reduced and oxidised N substrates are an important driver of exometabolome composition. Amino acids and peptides were the dominant compound class among metabolites that were significantly different between N treatments, but responses to N substrate were also reflected in altered extracellular concentrations of lipids, cyanotoxins, and photoprotectants. These differences in the molecular-level response to the type of N substrate supplied support that environmental factors like changing N availability and oxidative stress may synergistically influence M. aeruginosa strain fitness and community succession, as well as interactions between M. aeruginosa strains and other bacteria or cyanobacteria in the bloom community.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144930040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Environmental Detection of Balamuthia mandrillaris in Isfahan, Iran 伊朗伊斯法罕地区mandrillaris的分离与环境检测

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-01 DOI: 10.1111/1758-2229.70156

Sara Lesani, Soroush Mirzania, Abbasali Eskandarian

{"title":"Isolation and Environmental Detection of Balamuthia mandrillaris in Isfahan, Iran","authors":"Sara Lesani, Soroush Mirzania, Abbasali Eskandarian","doi":"10.1111/1758-2229.70156","DOIUrl":"10.1111/1758-2229.70156","url":null,"abstract":"Balamuthia mandrillaris is a free-living opportunistic amoeba known to cause fatal encephalitis. Despite its global environmental presence, data on its distribution in Iran and comparative detection methods remain scarce. This study aimed to investigate the occurrence of B. mandrillaris in various environmental sources in Isfahan, Iran, and to evaluate the performance of culture-based and molecular detection approaches. A total of 96 environmental samples (33 soil, 31 dust, and 32 water) were collected from different locations across Isfahan. All samples were examined in parallel using culture methods and PCR amplification targeting the mitochondrial 16S rDNA gene. Selected PCR-positive samples underwent sequencing to confirm species identity. The overall detection rate of B. mandrillaris was 15.6% using both culture and molecular techniques, with soil samples showing the highest prevalence (24.2% by molecular and 21.2% by culture). Molecular and cultural methods displayed complementary but matrix-dependent detection patterns, with a substantial agreement between methods (κ = 0.72). This study confirms the presence of B. mandrillaris in various environmental sources in Isfahan, highlighting soil as a potential reservoir. The findings emphasise the importance of combined molecular and culture-based approaches and suggest raising public health awareness, even in regions without reported clinical cases.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Environmental Detection of Balamuthia mandrillaris in Isfahan, Iran 伊朗伊斯法罕地区mandrillaris的分离与环境检测

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-01 DOI: 10.1111/1758-2229.70156

Sara Lesani, Soroush Mirzania, Abbasali Eskandarian

{"title":"Isolation and Environmental Detection of Balamuthia mandrillaris in Isfahan, Iran","authors":"Sara Lesani, Soroush Mirzania, Abbasali Eskandarian","doi":"10.1111/1758-2229.70156","DOIUrl":"10.1111/1758-2229.70156","url":null,"abstract":"Balamuthia mandrillaris is a free-living opportunistic amoeba known to cause fatal encephalitis. Despite its global environmental presence, data on its distribution in Iran and comparative detection methods remain scarce. This study aimed to investigate the occurrence of B. mandrillaris in various environmental sources in Isfahan, Iran, and to evaluate the performance of culture-based and molecular detection approaches. A total of 96 environmental samples (33 soil, 31 dust, and 32 water) were collected from different locations across Isfahan. All samples were examined in parallel using culture methods and PCR amplification targeting the mitochondrial 16S rDNA gene. Selected PCR-positive samples underwent sequencing to confirm species identity. The overall detection rate of B. mandrillaris was 15.6% using both culture and molecular techniques, with soil samples showing the highest prevalence (24.2% by molecular and 21.2% by culture). Molecular and cultural methods displayed complementary but matrix-dependent detection patterns, with a substantial agreement between methods (κ = 0.72). This study confirms the presence of B. mandrillaris in various environmental sources in Isfahan, highlighting soil as a potential reservoir. The findings emphasise the importance of combined molecular and culture-based approaches and suggest raising public health awareness, even in regions without reported clinical cases.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Interaction Between the Nematode Caenorhabditis elegans and Its Coexisting Fungal Microbiome Member Barnettozyma californica 秀丽隐杆线虫与共生真菌微生物组成员加利福尼亚巴奈特菌的相互作用

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-19 DOI: 10.1111/1758-2229.70177

Carola Petersen, Hanne Griem-Krey, Christina Martínez Christophersen, Hinrich Schulenburg, Michael Habig

{"title":"The Interaction Between the Nematode Caenorhabditis elegans and Its Coexisting Fungal Microbiome Member Barnettozyma californica","authors":"Carola Petersen, Hanne Griem-Krey, Christina Martínez Christophersen, Hinrich Schulenburg, Michael Habig","doi":"10.1111/1758-2229.70177","DOIUrl":"10.1111/1758-2229.70177","url":null,"abstract":"The nematode Caenorhabditis elegans is known to feed on and interact with bacteria in its environment and has become a model organism for microbiome studies. However, whether and how C. elegans interacts with co-occurring fungi remains largely unknown, despite the presence of many fungal species in its natural habitat. Here, we isolate the yeast Barnettozyma californica from a mesocosm experiment with C. elegans and characterise its genome and interaction with the nematode. We find that, like bacterial microbiota, B. californica can colonise the intestine of C. elegans and can serve as a sole, albeit poor, food source for adult nematodes. Yet, when present together with Escherichia coli OP50, the fungus can lead to higher population growth and altered foraging behaviour, suggesting a context-dependent benefit. This effect varied between different natural C. elegans strains, suggesting a genomic basis for the nematode's interaction with B. californica. On the fungal side, we could not identify any obvious candidate genes for its interaction with C. elegans and/or E. coli OP50, despite obtaining a fully assembled and annotated genome of the isolated B. californica strain. Overall, our results provide an intriguing example of the complexity and multi-level relationship between naturally interacting fungi, bacteria and animals.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5 假单胞菌蛋白Pf-5对植物根定植的遗传驱动

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-19 DOI: 10.1111/1758-2229.70179

Timothy M. Ghaly, Belinda K. Fabian, Silas H. W. Vick, Christie Foster, Amy J. Asher, Karl A. Hassan, Liam D. H. Elbourne, Ian T. Paulsen, Sasha G. Tetu

{"title":"Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5","authors":"Timothy M. Ghaly, Belinda K. Fabian, Silas H. W. Vick, Christie Foster, Amy J. Asher, Karl A. Hassan, Liam D. H. Elbourne, Ian T. Paulsen, Sasha G. Tetu","doi":"10.1111/1758-2229.70179","DOIUrl":"10.1111/1758-2229.70179","url":null,"abstract":"Plant growth-promoting bacteria can confer a range of health benefits to plants, and are increasingly being used in agriculture as bioinoculants to enhance crop performance and prevent diseases. However, within complex rhizosphere communities, their success as bioinoculants depends heavily on their capacity to competitively colonise root systems. Here, we uncover genetic determinants of root colonisation by the biocontrol agent Pseudomonas protegens Pf-5, known for its ability to suppress multiple plant diseases. Using Transposon-Directed Insertion Site Sequencing (TraDIS), we systematically assayed the entire P. protegens Pf-5 genome to determine genes involved in colonising the rhizoplane of two key agricultural crops, cotton (n = 153 Pf-5 genes) and wheat (n = 110 Pf-5 genes). We find a large overlap of 80 P. protegens Pf-5 genes which are important for colonisation fitness in both plants, suggesting that these encode core traits linked to root colonisation. In-depth functional annotation of these genes, leveraging both protein sequence and structure, reveals key traits that promote P. protegens Pf-5 rhizoplane fitness, including biofilm formation, surface motility, nucleotide and amino acid biosynthesis, sugar catabolism, iron uptake, low-oxygen growth, and stress response mechanisms. These findings can help guide future design and selection of microbial inoculants with improved capacity for competitive root colonisation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidden Allies: Decoding the Core Endohyphal Bacteriome of Aspergillus fumigatus 隐藏的盟友：解码烟曲霉核心菌丝内菌群

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-19 DOI: 10.1111/1758-2229.70153

Daryna Piontkivska, João M. P. Jorge, Dalila Mil-Homens, Tiago M. Martins, Pedro Crespo, Demosthenes P. Morales, Dinah Carvalho, José Melo-Cristino, Raquel Sá-Leão, Gustavo H. Goldman, Cristina Silva Pereira

{"title":"Hidden Allies: Decoding the Core Endohyphal Bacteriome of Aspergillus fumigatus","authors":"Daryna Piontkivska, João M. P. Jorge, Dalila Mil-Homens, Tiago M. Martins, Pedro Crespo, Demosthenes P. Morales, Dinah Carvalho, José Melo-Cristino, Raquel Sá-Leão, Gustavo H. Goldman, Cristina Silva Pereira","doi":"10.1111/1758-2229.70153","DOIUrl":"10.1111/1758-2229.70153","url":null,"abstract":"Bacterial–fungal interactions that influence the behaviour of one or both organisms are common in nature. Well-studied systems include endosymbiotic relationships that range from transient to long-term associations. Diverse endohyphal bacteria associate with fungal hosts, emphasising the need to better comprehend the fungal bacteriome. We evaluated the hypothesis that Aspergillus fumigatus harbours an endohyphal community of bacteria that influence the host phenotype. We analysed whether 38 A. fumigatus strains show stable association with diverse endohyphal bacteria; all derived from single-conidium cultures that were subjected to antibiotic and heat treatments. The fungal bacteriome, inferred through analysis of bacterial diversity within the fungal strains (short- and long- read sequencing methods), revealed the presence of core endohyphal bacterial genera. Microscopic analysis further confirmed the presence of endohyphal bacteria. The fungal strains exhibited high genetic diversity and phenotypic heterogeneity in drug susceptibility and in vivo virulence. No correlations were observed between genomic or functional traits and bacteriome diversity, but the abundance of some bacterial genera correlated with fungal virulence or posaconazole susceptibility. The observed endobacteriome may play functional roles, for example, nitrogen fixation. Our study emphasises the existence of complex interactions between fungi and endohyphal bacteria, possibly impacting the phenotype of the fungal host, including virulence.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0