Environmental Microbiology Reports最新文献_第5页

Root inoculation with soil-borne microorganisms alters gut bacterial communities and performance of the leaf-chewer Spodoptera exigua 根部接种土生微生物可改变啃叶蛾的肠道细菌群落和表现

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-26 DOI: 10.1111/1758-2229.70049

Beatriz Ramírez-Serrano, Marina Querejeta, Zhivko Minchev, María J. Pozo, Géraldine Dubreuil, David Giron

{"title":"Root inoculation with soil-borne microorganisms alters gut bacterial communities and performance of the leaf-chewer Spodoptera exigua","authors":"Beatriz Ramírez-Serrano, Marina Querejeta, Zhivko Minchev, María J. Pozo, Géraldine Dubreuil, David Giron","doi":"10.1111/1758-2229.70049","DOIUrl":"https://doi.org/10.1111/1758-2229.70049","url":null,"abstract":"Soil-borne microorganisms can impact leaf-chewing insect fitness by modifying plant nutrition and defence. Whether the altered insect performance is linked to changes in microbial partners of caterpillars remains unclear. We investigated the effects of root inoculation with soil bacteria or fungi on the gut bacterial community and biomass of the folivore Spodoptera exigua. We also explored the potential correlation between both parameters. We performed herbivory bioassay using leaves of tomato plants (Solanum lycopersicum), measured caterpillar weight gain and characterized the gut bacterial communities via 16S rRNA gene metabarcoding. All soil microbes modified the gut bacterial communities, but the extent of these changes depended on the inoculated species. Rhizophagus irregularis and Bacillus amyloliquefaciens had opposite effects on S. exigua weight. While plant inoculation with the fungus influenced gut bacterial diversity, B. amyloliquefaciens also affected the community composition. A reduced abundance of two S. exigua enterococcal symbionts correlated with decreased insect biomass. Our results show that soil microorganisms can induce plant-mediated changes in the gut bacterial community of foliar-feeding caterpillars. We propose that the impact of these alterations on insect performance might rely on specific adaptations within the gut bacteria, rather than solely on the occurrence of changes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root inoculation with soil-borne microorganisms alters gut bacterial communities and performance of the leaf-chewer Spodoptera exigua 根部接种土生微生物可改变啃叶蛾的肠道细菌群落和表现

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-26 DOI: 10.1111/1758-2229.70049

Beatriz Ramírez-Serrano, Marina Querejeta, Zhivko Minchev, María J. Pozo, Géraldine Dubreuil, David Giron

{"title":"Root inoculation with soil-borne microorganisms alters gut bacterial communities and performance of the leaf-chewer Spodoptera exigua","authors":"Beatriz Ramírez-Serrano, Marina Querejeta, Zhivko Minchev, María J. Pozo, Géraldine Dubreuil, David Giron","doi":"10.1111/1758-2229.70049","DOIUrl":"https://doi.org/10.1111/1758-2229.70049","url":null,"abstract":"Soil-borne microorganisms can impact leaf-chewing insect fitness by modifying plant nutrition and defence. Whether the altered insect performance is linked to changes in microbial partners of caterpillars remains unclear. We investigated the effects of root inoculation with soil bacteria or fungi on the gut bacterial community and biomass of the folivore Spodoptera exigua. We also explored the potential correlation between both parameters. We performed herbivory bioassay using leaves of tomato plants (Solanum lycopersicum), measured caterpillar weight gain and characterized the gut bacterial communities via 16S rRNA gene metabarcoding. All soil microbes modified the gut bacterial communities, but the extent of these changes depended on the inoculated species. Rhizophagus irregularis and Bacillus amyloliquefaciens had opposite effects on S. exigua weight. While plant inoculation with the fungus influenced gut bacterial diversity, B. amyloliquefaciens also affected the community composition. A reduced abundance of two S. exigua enterococcal symbionts correlated with decreased insect biomass. Our results show that soil microorganisms can induce plant-mediated changes in the gut bacterial community of foliar-feeding caterpillars. We propose that the impact of these alterations on insect performance might rely on specific adaptations within the gut bacteria, rather than solely on the occurrence of changes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite 比较不同 DNA 提取方法在 MX-80 膨润土中的效果

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-24 DOI: 10.1111/1758-2229.70047

Kristel Mijnendonckx, Carla Smolders, Deepa Bartak, Trung Le Duc, Mar Morales-Hidalgo, Cristina Povedano-Priego, Fadwa Jroundi, Mohamed L. Merroun, Natalie Leys, Katerina Cerna

{"title":"Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite","authors":"Kristel Mijnendonckx, Carla Smolders, Deepa Bartak, Trung Le Duc, Mar Morales-Hidalgo, Cristina Povedano-Priego, Fadwa Jroundi, Mohamed L. Merroun, Natalie Leys, Katerina Cerna","doi":"10.1111/1758-2229.70047","DOIUrl":"https://doi.org/10.1111/1758-2229.70047","url":null,"abstract":"Approaches to DNA extraction play a crucial role in determining the variability of results obtained through 16S rRNA amplicon sequencing. Particularly, clay-rich samples can impede the efficiency of various standard cultivation-independent techniques. We conducted an inter-laboratory comparison study to thoroughly assess the efficacy of two published DNA extraction methods (kit-based and phenol-chloroform-based) specifically designed for bentonite samples. To this end, we spiked Wyoming MX 80 bentonite with two different mock communities and compared the obtained DNA yield and purity, the presence of contaminants and the community profile. Our findings suggest that both methods are equally viable, with the best choice depending on the specific requirements of the downstream analysis. However, it is crucial to maintain consistency in the chosen method, as comparing results becomes challenging, particularly in the presence of bentonite. In summary, our study emphasizes the significance of standardized DNA extraction methods and underscores the importance of validating these methods using appropriate controls when studying microbial communities with 16S rRNA amplicon sequencing, particularly in environments characterized by low biomass and clay-rich compositions. Additionally, slight modifications to one of the extraction methods can substantially enhance its efficiency.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite 比较不同 DNA 提取方法在 MX-80 膨润土中的效果

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-24 DOI: 10.1111/1758-2229.70047

Kristel Mijnendonckx, Carla Smolders, Deepa Bartak, Trung Le Duc, Mar Morales-Hidalgo, Cristina Povedano-Priego, Fadwa Jroundi, Mohamed L. Merroun, Natalie Leys, Katerina Cerna

{"title":"Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite","authors":"Kristel Mijnendonckx, Carla Smolders, Deepa Bartak, Trung Le Duc, Mar Morales-Hidalgo, Cristina Povedano-Priego, Fadwa Jroundi, Mohamed L. Merroun, Natalie Leys, Katerina Cerna","doi":"10.1111/1758-2229.70047","DOIUrl":"https://doi.org/10.1111/1758-2229.70047","url":null,"abstract":"Approaches to DNA extraction play a crucial role in determining the variability of results obtained through 16S rRNA amplicon sequencing. Particularly, clay-rich samples can impede the efficiency of various standard cultivation-independent techniques. We conducted an inter-laboratory comparison study to thoroughly assess the efficacy of two published DNA extraction methods (kit-based and phenol-chloroform-based) specifically designed for bentonite samples. To this end, we spiked Wyoming MX 80 bentonite with two different mock communities and compared the obtained DNA yield and purity, the presence of contaminants and the community profile. Our findings suggest that both methods are equally viable, with the best choice depending on the specific requirements of the downstream analysis. However, it is crucial to maintain consistency in the chosen method, as comparing results becomes challenging, particularly in the presence of bentonite. In summary, our study emphasizes the significance of standardized DNA extraction methods and underscores the importance of validating these methods using appropriate controls when studying microbial communities with 16S rRNA amplicon sequencing, particularly in environments characterized by low biomass and clay-rich compositions. Additionally, slight modifications to one of the extraction methods can substantially enhance its efficiency.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased antibiotic resistance gene abundance linked to intensive bacterial competition in the phyllosphere across an elevational gradient 抗生素抗性基因丰度的增加与跨海拔梯度植物圈中激烈的细菌竞争有关。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-21 DOI: 10.1111/1758-2229.70042

Yihui Ding, Rui-Ao Ma, Ran Zhang, Hongwei Zhang, Jian Zhang, Shaopeng Li, Si-Yu Zhang

{"title":"Increased antibiotic resistance gene abundance linked to intensive bacterial competition in the phyllosphere across an elevational gradient","authors":"Yihui Ding, Rui-Ao Ma, Ran Zhang, Hongwei Zhang, Jian Zhang, Shaopeng Li, Si-Yu Zhang","doi":"10.1111/1758-2229.70042","DOIUrl":"10.1111/1758-2229.70042","url":null,"abstract":"Antibiotic resistance genes (ARGs) are ancient and widespread in natural habitats, providing survival advantages for microbiomes under challenging conditions. In mountain ecosystems, phyllosphere bacterial communities face multiple stress conditions, and the elevational gradients of mountains represent crucial environmental gradients for studying biodiversity distribution patterns. However, the distribution patterns of ARGs in the phyllosphere along elevational gradients, and their correlation with bacterial community structures, remain poorly understood. Here, we applied metagenomic analyses to investigate the abundance and diversity of ARGs in 88 phyllosphere samples collected from Mount Tianmu, a national natural reserve. Our results showed that the abundance of ARGs in the phyllosphere increased along elevational gradients and was dominated by multidrug resistance and efflux pumps. The composition of bacterial communities, rather than plant traits or abiotic factors, significantly affected ARG abundance. Moreover, increased ARG abundance was correlated with greater phylogenetic overdispersion and a greater proportion of negative associations in the bacterial co-occurrence networks, suggesting that bacterial competition primarily shapes phyllosphere resistomes. These findings constitute a major advance in the biodiversity of phyllosphere resistomes along elevations, emphasizing the significant impact of bacterial community structure and assembly on ARG distribution, and are essential for understanding the emergence of ARGs.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the tolerance of halophilic archaea to stress landscapes 了解嗜卤古细菌对压力景观的耐受性。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-20 DOI: 10.1111/1758-2229.70039

Laura Matarredona, Basilio Zafrilla, Mónica Camacho, María-José Bonete, Julia Esclapez

{"title":"Understanding the tolerance of halophilic archaea to stress landscapes","authors":"Laura Matarredona, Basilio Zafrilla, Mónica Camacho, María-José Bonete, Julia Esclapez","doi":"10.1111/1758-2229.70039","DOIUrl":"10.1111/1758-2229.70039","url":null,"abstract":"Haloarchaea, known for their resilience to environmental fluctuations, require a minimum salt concentration of 10% (w/v) for growth and can survive up to 35% (w/v) salinity. In biotechnology, these halophiles have diverse industrial applications. This study investigates the tolerance responses of nine haloarchaea: Haloferax mediterranei, Haloferax volcanii, Haloferax gibbonsii, Halorubrum californiense, Halorubrum litoreum, Natrinema pellirubrum, Natrinema altunense, Haloterrigena thermotolerans and Haloarcula sinaiiensis, under various stressful conditions. All these archaea demonstrated the ability to thrive in the presence of toxic metals such as chromium, nickel, cobalt and arsenic, and their tolerance to significantly elevated lithium concentrations in the medium was remarkable. Among the studied haloarchaea, Hfx. mediterranei exhibited superior resilience, particularly against lithium, with an impressive minimum inhibitory concentration (MIC) of up to 4 M LiCl, even replacing NaCl entirely. Haloferax species showed specificity for conditions with maximal growth rates, while Htg. thermotolerans and Nnm. altunense displayed high resilience without losing growth throughout the ranges, although these were generally low. ICP-MS results highlighted the impressive intracellular lithium accumulation in Nnm. pellirubrum, emphasizing its potential significance in bioremediation. This research highlights a new characteristic of haloarchaea, their tolerance to high lithium concentrations and the potential for new applications in extreme industrial processes and bioremediation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic analysis reveals houseflies as indicators for monitoring environmental antibiotic resistance genes 元基因组分析发现家蝇是监测环境抗生素耐药性基因的指标。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-19 DOI: 10.1111/1758-2229.70032

Yuhan Yang, Ping Xu, Wei He, Fei Tao

{"title":"Metagenomic analysis reveals houseflies as indicators for monitoring environmental antibiotic resistance genes","authors":"Yuhan Yang, Ping Xu, Wei He, Fei Tao","doi":"10.1111/1758-2229.70032","DOIUrl":"10.1111/1758-2229.70032","url":null,"abstract":"Given the threat to public health posed by antibiotic resistance transmission, environmental monitoring is essential for tracking antibiotic resistance genes (ARGs). Houseflies, being ubiquitous organisms capable of carrying and disseminating ARGs, serve as suitable indicators for environmental monitoring. In this study, we employ metagenomic approaches to investigate housefly body surface samples from five typical sites associated with human activities. The investigation reveals microbiome diversity among the samples, along with variations in the occurrence and mobility potential of ARGs. Metagenomic analysis indicates that the composition of ARGs on housefly body surfaces is influenced by environmental ARGs, which may be enriched on the housefly body surface. The resistance genes related to multidrug, β-lactam, bacitracin, and tetracycline were the predominant ARGs detected, with multidrug-related ARGs consistently exhibiting dominance. Furthermore, the abundance of ARGs in the different housefly body surface samples was found to correlate with the population density and mobility of the sampling site. Natural environments exhibited the lowest ARG abundance, while areas with higher population density and limited population mobility displayed higher ARG abundance. This study emphasizes the effectiveness of houseflies as monitors for environmental ARGs and underscores their potential for assessing and controlling antibiotic resistance risks in urban environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

At what cost? The impact of bacteriophage resistance on the growth kinetics and protein synthesis of Escherichia coli 代价是什么？噬菌体抗药性对大肠杆菌生长动力学和蛋白质合成的影响。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-19 DOI: 10.1111/1758-2229.70046

Lotta A. I. Landor, Jesslyn Tjendra, Karen Erstad, Anders K. Krabberød, Joachim P. Töpper, Selina Våge

{"title":"At what cost? The impact of bacteriophage resistance on the growth kinetics and protein synthesis of Escherichia coli","authors":"Lotta A. I. Landor, Jesslyn Tjendra, Karen Erstad, Anders K. Krabberød, Joachim P. Töpper, Selina Våge","doi":"10.1111/1758-2229.70046","DOIUrl":"10.1111/1758-2229.70046","url":null,"abstract":"Cost of bacteriophage resistance (COR) is important in explaining processes of diversification and coexistence in microbial communities. COR can be expressed in different traits, and the lack of universally applicable methods to measure fitness trade-offs makes COR challenging to study. Due to its fundamental role in growth, we explored protein synthesis as a target for quantifying COR. In this study, the growth kinetics of three genome-sequenced strains of phage-resistant Escherichia coli, along with the phage-susceptible wild-type, were characterized over a range of glucose concentrations. Bioorthogonal non-canonical amino acid tagging (BONCAT) was used to track differences in protein synthetic activity between the wild-type and phage-resistant E. coli. Two of the resistant strains, with different levels of phage susceptibility, showed mucoid phenotypes corresponding with mutations in genes associated with the Rcs phosphorelay. These mucoid isolates, however, had reduced growth rates and potentially lower protein synthetic activity. Another resistant isolate with a different mutational profile maintained the same growth rate as the wild-type and showed increased BONCAT fluorescence, but its yield was lower. Together, these findings present different patterns of trade-offs resulting from the phage-induced mutations and demonstrate the potential applicability of BONCAT as a tool for measuring COR.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1,8-Dihydroxynaphthalene (DHN) melanin provides unequal protection to black fungi Knufia petricola and Cryomyces antarcticus from UV-B radiation 1,8-二羟基萘（DHN）黑色素对黑色真菌 Knufia petricola 和 Cryomyces antarcticus 免受紫外线-B 辐射的保护作用是不平等的。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-15 DOI: 10.1111/1758-2229.70043

Ilaria Catanzaro, Anna A. Gorbushina, Silvano Onofri, Julia Schumacher

{"title":"1,8-Dihydroxynaphthalene (DHN) melanin provides unequal protection to black fungi Knufia petricola and Cryomyces antarcticus from UV-B radiation","authors":"Ilaria Catanzaro, Anna A. Gorbushina, Silvano Onofri, Julia Schumacher","doi":"10.1111/1758-2229.70043","DOIUrl":"10.1111/1758-2229.70043","url":null,"abstract":"Black fungi on rock surfaces endure a spectrum of abiotic stresses, including UV radiation. Their ability to tolerate extreme conditions is attributed to the convergent evolution of adaptive traits, primarily highly melanized cell walls. However, studies on fungal melanins have not provided univocal results on their photoprotective functions. Here, we investigated whether the black fungi Knufia petricola and Cryomyces antarcticus only use DHN melanin or may employ alternative mechanisms to counteract UV-induced damage. For this, melanized wild types and non-melanized Δpks1 mutants were exposed to different doses of UV-B (312 nm) followed by incubation in constant darkness or in light–dark cycles to allow light-dependent DNA repair by photolyases (photoreactivation). C. antarcticus could tolerate higher UV-B doses but was sensitive to white light, whereas K. petricola showed the opposite trend. DHN melanin provided UV-B protection in C. antarcticus, whereas the same pigment or even carotenoids proved ineffective in K. petricola. Both fungi demonstrated functional photoreactivation in agreement with the presence of photolyase-encoding genes. Our findings reveal that although the adaptive trait of DHN melanization commonly occurs across black fungi, it is not equally functional and that there are species-specific adaptations towards either UV-induced lesion avoidance or repair strategies.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11567843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct microbial communities associated with health-relevant wild berries 与有益健康的野生浆果相关的独特微生物群落。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-11-14 DOI: 10.1111/1758-2229.70048

Iglė Vepštaitė-Monstavičė, Juliana Lukša, Živilė Strazdaitė-Žielienė, Saulius Serva, Elena Servienė

{"title":"Distinct microbial communities associated with health-relevant wild berries","authors":"Iglė Vepštaitė-Monstavičė, Juliana Lukša, Živilė Strazdaitė-Žielienė, Saulius Serva, Elena Servienė","doi":"10.1111/1758-2229.70048","DOIUrl":"10.1111/1758-2229.70048","url":null,"abstract":"Lingonberries (Vaccinium vitis-idaea L.), rowanberries (Sorbus aucuparia L.) and rosehips (Rosa canina L.) positively affect human health due to their healing properties, determined by a high content of bioactive compounds. The consumption of unprocessed wild berries is relevant and encouraged, making their in-depth microbiological characterization essential for food safety. This study presents the first high-throughput sequencing analysis of bacterial and fungal communities distributed on the surface of lingonberries, rowanberries and rosehips. Significant plant-defined differences in the taxonomic composition of prokaryotic and eukaryotic microbiota were observed. The bacterial community on rosehips was shown to be prevalent by Enterobacteriaceae, lingonberries by Methylobacteriaceae and rowanberries by Sphingomonadaceae representatives. Among the fungal microbiota, Dothioraceae dominated on rosehips and Exobasidiaceae on lingonberries; meanwhile, rowanberries were inhabited by a similar level of a broad spectrum of fungal families. Cultivable yeast profiling revealed that lingonberries were distinguished by the lowest amount and most distinct yeast populations. Potentially pathogenic to humans or plants, as well as beneficial and relevant biocontrol microorganisms, were identified on tested berries. The combination of metagenomics and a cultivation-based approach highlighted the wild berries-associated microbial communities and contributed to uncovering their potential in plant health, food and human safety.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0