Environmental Microbiology Reports最新文献_第5页

Soil Microbiomes Across Depth and Ecosystems in Dubai, UAE: Potential Environmental Signatures for Forensic Geolocation 阿联酋迪拜深度和生态系统的土壤微生物组：法医地理定位的潜在环境特征

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-14 DOI: 10.1111/1758-2229.70403

Abdulla Albastaki, Mohammed Naji, Mohamed Moussa, Judith Smith

{"title":"Soil Microbiomes Across Depth and Ecosystems in Dubai, UAE: Potential Environmental Signatures for Forensic Geolocation","authors":"Abdulla Albastaki, Mohammed Naji, Mohamed Moussa, Judith Smith","doi":"10.1111/1758-2229.70403","DOIUrl":"https://doi.org/10.1111/1758-2229.70403","url":null,"abstract":"Soil microbial communities exhibit strong sensitivity to environmental gradients, yet their distribution across depth and land-use types in hyper-arid environments remains poorly characterised. Using whole-genome shotgun metagenomics via Oxford Nanopore Technologies long-read sequencing, we profiled soil microbial communities across six contrasting land-use sites in Dubai, UAE: urban, industrial (two locations), marine, desert and agricultural, where each sampled at three depth intervals (0–25 cm, 25–50 cm and 50–100 cm). Marine soils exhibited extreme salinity (EC 23.7–30.7 dS m−1) and the highest organic matter content (1.19%–1.76%), while desert soils were nutrient-poor with minimal salinity. Actinomycetota and Pseudomonadota co-dominated across all sites, collectively accounting for 77%–96% of classified sequences. Actinomycetota prevailed in undisturbed desert horizons (up to 53.4%), while Pseudomonadota dominated nutrient-enriched environments, reaching 69.4% at industrial sites. A notable compositional reversal was observed in the desert deep horizon (50–100 cm), where Pseudomonadota increased to 56.8%, departing from the expected oligotrophic depth gradient. PERMANOVA confirmed land use as the primary driver of community composition (p = 0.001), with depth exerting a secondary but significant effect (p ≤ 0.01). NMDS ordination revealed strong site-specific clustering, with each environment harbouring a distinctive microbial fingerprint with promising forensic geolocation potential.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging Microbes for Sustainable Development Goal 14 Progress 利用微生物促进可持续发展目标14的进展。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-11 DOI: 10.1111/1758-2229.70399

Reshum Aurora, John Paul Balmonte

{"title":"Leveraging Microbes for Sustainable Development Goal 14 Progress","authors":"Reshum Aurora, John Paul Balmonte","doi":"10.1111/1758-2229.70399","DOIUrl":"10.1111/1758-2229.70399","url":null,"abstract":"To promote sustainable use of marine resources, the United Nations Sustainable Development Goal 14 (SDG 14): Life Below Water aims to alleviate the threats of marine pollution, habitat degradation, unsustainable fishing and ocean acidification on ocean ecosystems. With the 2030 deadline approaching, much work remains, necessitating innovative solutions for ocean health and sustainability that can be upscaled and implemented globally. The metabolic versatility of microorganisms makes them powerful, yet currently underutilized tools that can be leveraged to advance SDG 14. Here, we synthesize current microbial technologies that align with primary targets of SDG 14: plastic reduction (14.1), nutrient removal (14.1), coastal restoration (14.2) and sustainable aquaculture (14.7). Diverse microbial groups are involved in these functions in natural and human-perturbed ecosystems, including plastic-degrading bacteria, denitrifying communities, host-associated microbes, biofloc and periphyton systems, as well as photosynthetic microorganisms. We emphasize the ways in which wide-scale implementation requires mechanistic and systems-level understanding of microbial applications, environmental testing in variable environments and the development of monitoring and deployment infrastructure. We also highlight the importance of protecting microbial biodiversity to preserve the ecosystem services that will maintain the outcomes of SDG 14. Integrating microorganisms into existing ocean management and policy structures represents a compelling pathway to accelerate progress towards SDG 14—a conceptual framework that can be applied to other SDGs.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopore Long-Read Metagenomics Reveals Pollution-Driven Antibiotic Resistance and Xenobiotic Degradation in Urban Beach Microbiomes 纳米孔长读宏基因组学揭示了污染驱动的抗生素耐药性和城市海滩微生物群落的外源降解。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-10 DOI: 10.1111/1758-2229.70396

Angelo Felipe Barbosa de Oliveira, Bruno Santana Carneiro, Joelma Barbosa de Carvalho, Adan Rodrigues de Oliveira, Artur Luiz da Costa da Silva, Adonney Allan de Oliveira Veras, Rafael Azevedo Baraúna, Diego Assis das Graças

{"title":"Nanopore Long-Read Metagenomics Reveals Pollution-Driven Antibiotic Resistance and Xenobiotic Degradation in Urban Beach Microbiomes","authors":"Angelo Felipe Barbosa de Oliveira, Bruno Santana Carneiro, Joelma Barbosa de Carvalho, Adan Rodrigues de Oliveira, Artur Luiz da Costa da Silva, Adonney Allan de Oliveira Veras, Rafael Azevedo Baraúna, Diego Assis das Graças","doi":"10.1111/1758-2229.70396","DOIUrl":"10.1111/1758-2229.70396","url":null,"abstract":"Coastal ecosystems are vital for biodiversity but are increasingly threatened by urbanisation and pollution, which significantly alter local microbial communities. This study assessed bacterial diversity and functional profiles in urban and island beaches in Belém, Brazil. Urban beaches showed significantly higher microbial diversity and evenness, alongside functional plasticity due to pollutant input, while island beaches hosted more specialised and stable communities. Taxonomic analysis revealed the significant enrichment of opportunistic genera such as Comamonas, Clostridium and Paenibacillus in urban areas, and the massive dominance of Prochlorococcus and Candidatus Pelagibacter in island sites. Furthermore, shotgun metagenomics identified a robust genomic potential for xenobiotic degradation and antibiotic resistance in urban microbiomes, whereas island microbiomes were significantly enriched in genes for energy production and biosynthesis. These results underscore the ecological divergence between anthropogenically impacted and natural coastal environments, highlighting the importance of microbiome monitoring for sustainable coastal management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Most of the Specific Genomic Regions of Agrobacterium fabrum Shape Phenolics Content of Medicago truncatula Roots 短截紫花苜蓿根中农杆菌形状酚类物质含量的大部分特定基因组区域。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-07 DOI: 10.1111/1758-2229.70394

Rosa Padilla, Ludovic Vial, Florence Dechèvre, Thi Huyen Thu Nguyen, Deniz Sarigol, Vincent Gaillard, Guillaume Meiffren, Gilles Comte, Xavier Nesme, Céline Lavire, Isabelle Kerzaon

{"title":"Most of the Specific Genomic Regions of Agrobacterium fabrum Shape Phenolics Content of Medicago truncatula Roots","authors":"Rosa Padilla, Ludovic Vial, Florence Dechèvre, Thi Huyen Thu Nguyen, Deniz Sarigol, Vincent Gaillard, Guillaume Meiffren, Gilles Comte, Xavier Nesme, Céline Lavire, Isabelle Kerzaon","doi":"10.1111/1758-2229.70394","DOIUrl":"10.1111/1758-2229.70394","url":null,"abstract":"The impact of plant microbiota on host is increasingly recognized, yet specific bacterial functions and genetic determinants in rhizosphere interactions remain largely unknown. Agrobacteria, common soil and rhizospheric bacteria, establish root interactions. The species Agrobacterium fabrum harbour seven specific-gene regions, whose annotation indicates a close connection with the plant. To evaluate the involvement of these specific regions in the plant-bacteria interaction, deletion mutant strains of each specific region were inoculated on Medicago truncatula roots. Root metabolite profiles were compared by UHPLC-UV/DAD-ESI-MS QTOF analyses, and the highlighted discriminating metabolites were annotated by tandem mass spectrometry. A. fabrum inoculation modulates the content of root phenolic compounds, in particular flavonoids. These root metabolite modulations appear to be linked to at least one of the A. fabrum-specific genes, as almost all specific regions showed an influence on one or more of these metabolites. Furthermore, our results suggested a putative cross-talk of the specific regions during the interaction of A. fabrum with M. truncatula, as all mutants except one induced similar modifications on flavonoids. These findings enhance our understanding of A. fabrum's ecological niche construction, highlighting the importance of specific genes in the establishment of this fine-tuned interaction.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13451346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Resistance of Serratia marcescens Recovered From Aquatic Environments: A Review 水生环境中粘质沙雷菌的耐药性研究进展

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-04 DOI: 10.1111/1758-2229.70345

Heloisa Silva Inacio, Yasmim Raffaela Soares Santos, Magna Cristina de Paiva, Jaqueline Maria Siqueira Ferreira

{"title":"Antimicrobial Resistance of Serratia marcescens Recovered From Aquatic Environments: A Review","authors":"Heloisa Silva Inacio, Yasmim Raffaela Soares Santos, Magna Cristina de Paiva, Jaqueline Maria Siqueira Ferreira","doi":"10.1111/1758-2229.70345","DOIUrl":"10.1111/1758-2229.70345","url":null,"abstract":"Serratia marcescens is a gram-negative bacillus commonly found in aquatic environments. This opportunistic human pathogen can form biofilms and acquire resistance to multiple antimicrobial classes, raising public and environmental health concerns. The occurrence of antimicrobial-resistant S. marcescens in aquatic ecosystems is alarming, yet relatively few studies have addressed this topic in depth. To compile and analyse studies on the antimicrobial susceptibility profiles and resistance mechanisms of S. marcescens isolated from aquatic environments. A systematic search was performed in PubMed, Scopus, Web of Science and the Virtual Health Library for articles published between 2019 and 2024, using descriptors related to S. marcescens from aquatic sources. Studies evaluating susceptibility profiles of isolates from water samples were included. Thirteen articles met the inclusion criteria, encompassing 90 isolates from diverse water sources. Resistance to β-lactams was most prevalent, including carbapenems, the most recent compounds in this class. Identified mechanisms included plasmid-mediated genes blaOXA-48, blaTEM-1 and aac(6′)-Ic. Aquatic environments can serve as reservoirs for resistant S. marcescens, posing risks of dissemination to humans and other ecosystems. Continuous monitoring, environmental contamination control and stronger microbiological surveillance policies are crucial to mitigate this emerging public health threat.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Texture and Neonicotinoid Exposure Shape Bacterial Assemblages and Functions in Agricultural Soils: Responses Over Prolonged Exposure 质地和新烟碱暴露影响农业土壤中细菌组合和功能：对长时间暴露的反应。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-03 DOI: 10.1111/1758-2229.70395

Sharmin Akter, Julia Jasonsmith, Nilantha R. Hulugalle, Craig L. Strong, James O. Latimer

{"title":"Texture and Neonicotinoid Exposure Shape Bacterial Assemblages and Functions in Agricultural Soils: Responses Over Prolonged Exposure","authors":"Sharmin Akter, Julia Jasonsmith, Nilantha R. Hulugalle, Craig L. Strong, James O. Latimer","doi":"10.1111/1758-2229.70395","DOIUrl":"10.1111/1758-2229.70395","url":null,"abstract":"The physical and chemical properties of soil fundamentally shape its microbial communities. In a controlled 28-day microcosm experiment, we assessed bacterial community responses to imidacloprid in three soils with differing textures and classifications: a loamy sand (11 g/100 g clay; red Luvisol), a sandy loam (16 g/100 g clay; red Luvisol) and a clay soil (56 g/100 g clay, Vertisol). Analyses included 16S rRNA gene amplicon sequencing, indicator species analysis, co-occurrence network analysis and PICRUSt2-based functional prediction. Imidacloprid exposure elicited soil-specific shifts in bacterial community structure, primarily altering evenness rather than richness; however, overall diversity patterns were more strongly governed by soil texture and sampling time. Indicator species analysis identified distinct sensitive and tolerant taxa in each soil texture, with a core set of taxa remaining largely unchanged. Co-occurrence network analysis showed decreased network complexity and increased modularity under imidacloprid, particularly in loamy sand and clay soils, suggesting altered bacterial interaction patterns. Predicted functional profiles showed upregulation of stress-response pathways and downregulation of energy/nutrient metabolism pathways, implying a community-level shift toward stress adaptation. Although taxonomic richness remained relatively stable, these reorganisations of community interactions and functional potential suggest changes in bacterial resilience and biogeochemical cycling, which may have implications for long-term soil health.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13433008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting Indoor Bacteria Producing Bioactive Metabolites. 生物勘探室内细菌产生生物活性代谢物。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-01 DOI: 10.1111/1758-2229.70402

Maria A Andersson, Szabolcs Nagy, Balázs Kakasi, Raimo Mikkola, Heidi Salonen

{"title":"Bioprospecting Indoor Bacteria Producing Bioactive Metabolites.","authors":"Maria A Andersson, Szabolcs Nagy, Balázs Kakasi, Raimo Mikkola, Heidi Salonen","doi":"10.1111/1758-2229.70402","DOIUrl":"10.1111/1758-2229.70402","url":null,"abstract":"New biomolecules are prospected for new antimicrobial, antiparasitic and cytostatic drugs. Here, we present bioassay-guided methods for prospecting indoor bacteria producing non-protein antibiotic molecules. Out of 650 bacterial colonies tested, 108 antimicrobial isolates representing 15 species and cryptic Actinobacteria, produced non-protein antibiotics affecting mammalian cells. Methanol extracts of the 108 strains tested with a battery of bioassays revealed four toxicity endpoints: (1) Cell death (CD). (2) Boar sperm motility inhibition (BSMI). (3) Inhibition of cell proliferation (ICP). (4) Mitochondrial toxicity (MT). The battery of endpoints divided the 108 strains into four toxicity profiles: Profile I: Lethal toxins (42 strains). Positive controls: Alamethicin, a channel forming ionophore and a biosurfactant surfactin. Profile II: Sublethal mitochondrial toxins (51 strains). Positive controls: The mitochondrial toxins valinomycin and antimycin A. Profile III: Sublethal cytostatic toxins (7 strains). Positive control: Chaetoglobosin A, a glucose transport inhibitor. Profile IV: Cytostatic toxins (8 strains). Positive controls: Actinomycin D, RNA syntesis inhibitor. Strains of Bacillus, Streptomyces and Paenibacillus produced amylosin, cereulide, surfactins, lichenysins, iturins, fengysin, valinomycin and fusarisidins. Strains of Peribacillus Lysinibacillus, Microbacterium, Nocardiopsis and cryptic Actnobacteria produced possible novel bioactive molecules. The indoor environments were easily accessible habitats for bacteria producing ionophores, surfactants, and mitochondrial toxins.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":"e70402"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing the Resolution of the Sulfur Compound Disproportionation Puzzle in Dissulfuribacter Thermophilus S69ᵀ Through Quantitative Label-Free Comparative Proteomics. 通过定量无标记比较蛋白质组学推进嗜热二硫杆菌S69中硫化合物歧化之谜的解决

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-08-01 DOI: 10.1111/1758-2229.70406

Marilina Fernandez, Barbara Schoepp-Cothenet, Marie Hemon, Carine Rodrigues-Machado, Lukas V F Novák, Xavier Philippon, Sébastien Laurent, Céline Henry, Karine Alain

{"title":"Advancing the Resolution of the Sulfur Compound Disproportionation Puzzle in Dissulfuribacter Thermophilus S69ᵀ Through Quantitative Label-Free Comparative Proteomics.","authors":"Marilina Fernandez, Barbara Schoepp-Cothenet, Marie Hemon, Carine Rodrigues-Machado, Lukas V F Novák, Xavier Philippon, Sébastien Laurent, Céline Henry, Karine Alain","doi":"10.1111/1758-2229.70406","DOIUrl":"https://doi.org/10.1111/1758-2229.70406","url":null,"abstract":"In deep-sea hydrothermal vents, sulfur compounds are central for microbial bioenergetics. Despite its significant impact on the global sulfur cycle, anaerobic sulfur compound disproportionation remains the least understood metabolism, with only three bacterial strains having been investigated at the proteomic level for their sulfur disproportionation metabolism to date. Here, we present a comparative proteomics study of the chemolithoautotrophic sulfur-cycling species Dissulfuribacter thermophilus S69ᵀ. Upon thiosulfate disproportionation conditions, proteomics revealed a high abundance of a cytoplasmic thiosulfate reductase AB homologue (PhsAB-like) that might reductively cleave thiosulfate into sulfide and sulfite. Subsequent steps might involve enzymes from the dissimilatory sulfate reduction, with Apr and Sat potentially functioning in reverse to produce sulfate. Strain S69T exhibited enhanced growth under sulfite/dihydrogen, likely due to both sulfite respiration and disproportionation. Here, a range of new enzymes were more abundant. These included an enzyme containing NrfD, the MolyAB as well as two membranous tetrathionate reductase homologues. Other membranous oxidoreductases such as QmoABC, DsrMKJOP and Complex I, may contribute to the pathway, while autotrophic growth is likely sustained by the Wood-Ljungdahl pathway. These findings highlight the distinct enzymatic features of strain S69ᵀ compared to previously proteomically characterised sulfur-disproportionating strains, revealing novel enzymes that warrant further analysis.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":"e70406"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Development of Wild Microbiomes in the Eastern Fence Lizard 探索东部栅栏蜥蜴野生微生物群的发育。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-28 DOI: 10.1111/1758-2229.70373

Michael S. Grapin, Justin Wright, Jeremy Chen See, Samantha Anderson, Regina Lamendella, John Matter

{"title":"Exploring the Development of Wild Microbiomes in the Eastern Fence Lizard","authors":"Michael S. Grapin, Justin Wright, Jeremy Chen See, Samantha Anderson, Regina Lamendella, John Matter","doi":"10.1111/1758-2229.70373","DOIUrl":"10.1111/1758-2229.70373","url":null,"abstract":"This study examines the gut microbiome from faecal samples of a reptilian model (Sceloporus undulatus). The dynamic host-symbiote interactions between gut microbiota are essential in metabolic processes and overall health of the host organism. We used 16S rRNA gene sequencing to profile the bacterial community from the guts of wild Eastern Fence Lizards. Microbiome profiles were collected at four distinct time points when lizards were actively foraging in the environment and across four age groups, aiming to characterise the natural variation in bacterial diversity. Environmental bacteria such as Mycobacterium and Pantoea in young of year (YOY) lizards underwent successional microbiome changes to taxa including Caproiciproducens, Eubacterium, Fusobacterium and Roseburia in adults, reflecting dietary shifts from maternal nutrients and early-stage prey to fibre-rich arthropod diets. Juvenile lizards exhibited a simple gut microbiome dominated by pioneer colonisers (Firmicutes, Bacteroidota and Proteobacteria), with alpha diversity increasing rapidly within a month to near adult levels. This research extends our understanding of reptilian microbiomes, capturing a snapshot of the ecological complexities of Eastern Fence Lizard microbiomes. As wild lizards mature, their gut microbiome becomes more stable and resilient to environmental disturbances, developing a diverse and balanced bacterial community that supports functional redundancy and ecosystem stability.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148596814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeasts on Tree Gum: Taxonomic, Genetic and Phenotypic Diversity 树胶上的酵母菌：分类、遗传和表型多样性。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-28 DOI: 10.1111/1758-2229.70393

Matthias Sipiczki

{"title":"Yeasts on Tree Gum: Taxonomic, Genetic and Phenotypic Diversity","authors":"Matthias Sipiczki","doi":"10.1111/1758-2229.70393","DOIUrl":"10.1111/1758-2229.70393","url":null,"abstract":"Depending on their composition, exudates oozing from trees can support or inhibit the growth of microorganisms. Resins released by conifers have a strong antimicrobial effect. Sugar-rich saps are usually colonised by diverse microorganisms including yeasts. In this study, the yeasts associated with the polysaccharide-containing gums of cherry trees were examined. 227 strains were isolated from gums of 53 abandoned road-side and orchard sour cherry trees in 20 locations. 12 ascomycetous and 19 basidiomycetous species were found among the isolates. All grew very poorly on laboratory media containing gum as the only source of nutrients. The high taxonomic diversity and poor growth of the isolates on gum-based media indicate that the cherry-tree gum is unlikely to be permanently colonised by a characteristic yeast community. Strains of 16 species showed antifungal antagonism. The Metschnikowia pulcherrima isolates killed the cells of the test organisms by releasing pulcherriminic acid. These isolates showed high intragenomic barcode (D1/D2 domain and ITS) diversity due to single-nucleotide dimorphism (ambiguous nucleotides) in many sites. In the D1/D2 domain, the ambiguous sites were concentrated in four segments that correspond to the back-folding strands of the stems of the D1 and D2 loops of the secondary structure of the LSU rRNA.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148596873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0