Environmental Microbiology Reports最新文献_第5页

Investigating the resistome of haemolytic bacteria in Arctic soils 研究北极土壤中溶血细菌的抗药性基因组。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-23 DOI: 10.1111/1758-2229.70028

Diana C. Mogrovejo-Arias, Melanie C. Hay, Arwyn Edwards, Andrew C. Mitchell, Jörg Steinmann, Florian H. H. Brill, Bernd Neumann

{"title":"Investigating the resistome of haemolytic bacteria in Arctic soils","authors":"Diana C. Mogrovejo-Arias, Melanie C. Hay, Arwyn Edwards, Andrew C. Mitchell, Jörg Steinmann, Florian H. H. Brill, Bernd Neumann","doi":"10.1111/1758-2229.70028","DOIUrl":"10.1111/1758-2229.70028","url":null,"abstract":"Microorganisms inhabiting hostile Arctic environments express a variety of functional phenotypes, some of clinical interest, such as haemolytic ability and antimicrobial resistance. We studied haemolytic bacterial isolates from Arctic habitats, assessing their minimum inhibitory concentration (MIC) against antimicrobials. We then performed whole genome sequencing and analysed them for features conferring antimicrobial resistance. MIC data showed that Micromonospora spp. belong to 33% non-wild type (NWT) for erythromycin and penicillin and 22% NWT for tetracycline. Both Pseudomonas spp. belong to 43% NWT for nalidixic acid and streptomycin and 29% NWT for colistin. Finally, the Pedobacter isolate was in 80% NWT for antimicrobials tested. Whole-genome sequencing analyses revealed that fluoroquinolones, tetracyclines, macrolides and penams were the most frequent drug classes against which genotypic resistance was found. Additionally, resistance genes to heavy metals and disinfectants were identified. Our research demonstrates the presence of antimicrobial resistance in bacteria from Arctic habitats and highlights the importance of conservation efforts in these environments, where anthropogenic influence is becoming more evident. Furthermore, our data suggest the possible presence of novel resistance mechanisms, which could pose a threat if the responsible genes are transferable between species or become widespread due to environmental stress and alterations brought about by climate change.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low microbial abundance and community diversity in the egg capsule of the oviparous cloudy catshark (Scyliorhinus torazame) during oviposition 卵生云纹鲨（Scyliorhinus torazame）产卵期间卵囊内微生物丰度和群落多样性较低。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-22 DOI: 10.1111/1758-2229.70025

Wataru Takagi, Ayami Masuda, Koya Shimoyama, Kotaro Tokunaga, Susumu Hyodo, Yuki Sato-Takabe

{"title":"Low microbial abundance and community diversity in the egg capsule of the oviparous cloudy catshark (Scyliorhinus torazame) during oviposition","authors":"Wataru Takagi, Ayami Masuda, Koya Shimoyama, Kotaro Tokunaga, Susumu Hyodo, Yuki Sato-Takabe","doi":"10.1111/1758-2229.70025","DOIUrl":"10.1111/1758-2229.70025","url":null,"abstract":"Vertebrate embryos are protected from bacterial infection by various maternally derived factors, yet little is known about the defence mechanisms in elasmobranchs. This study aimed to characterize the intracapsular environment of freshly laid eggs of the oviparous catshark (Scyliorhinus torazame) by investigating the microbial abundance and microbiota to understand its potential contribution to embryonic defence. The egg capsule of oviparous elasmobranchs is tightly sealed until pre-hatching, after which seawater flows into the capsule, exposing the embryos to the surrounding seawater. We found that early embryos were highly vulnerable to environmental pathogens, suggesting that the embryos are somehow protected from infection before pre-hatching. Indeed, the intracapsular environment of freshly laid eggs exhibited significantly low bacterial density, maintained until pre-hatching. Furthermore, the microbiome inside eggs just after oviposition differed markedly from those of rearing seawater and adult oviducal gland epithelia; these eggs were predominantly populated by an unidentified genus of Sphingomonadaceae. Overall, this study provides compelling evidence that early embryos of oviparous cloudy catshark are incubated in a clean intracapsular environment that potentially plays a significant role in embryonic development in oviparous elasmobranchs.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acidophilic sulphate-reducing bacteria: Diversity, ecophysiology, and applications 嗜酸性硫酸盐还原菌：多样性、生态生理学和应用

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-13 DOI: 10.1111/1758-2229.70019

Luis Felipe Valdez-Nuñez, Andreas Kappler, Diana Ayala-Muñoz, Idelso Jamín Chávez, Muammar Mansor

{"title":"Acidophilic sulphate-reducing bacteria: Diversity, ecophysiology, and applications","authors":"Luis Felipe Valdez-Nuñez, Andreas Kappler, Diana Ayala-Muñoz, Idelso Jamín Chávez, Muammar Mansor","doi":"10.1111/1758-2229.70019","DOIUrl":"https://doi.org/10.1111/1758-2229.70019","url":null,"abstract":"Acidophilic sulphate-reducing bacteria (aSRB) are widespread anaerobic microorganisms that perform dissimilatory sulphate reduction and have key adaptations to tolerate acidic environments (pH <5.0), such as proton impermeability and Donnan potential. This diverse prokaryotic group is of interest from physiological, ecological, and applicational viewpoints. In this review, we summarize the interactions between aSRB and other microbial guilds, such as syntrophy, and their roles in the biogeochemical cycling of sulphur, iron, carbon, and other elements. We discuss the biotechnological applications of aSRB in treating acid mine drainage (AMD, pH <3), focusing on their ability to produce biogenic sulphide and precipitate metals, particularly in the context of utilizing microbial consortia instead of pure isolates. Metal sulphide nanoparticles recovered after AMD treatment have multiple potential technological uses, including in electronics and biomedicine, contributing to a cost-effective circular economy. The products of aSRB metabolisms, such as biominerals and isotopes, could also serve as biosignatures to understand ancient and extant microbial life in the universe. Overall, aSRB are active components of the sulphur and carbon cycles under acidic conditions, with potential natural and technological implications for the world around us.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1 新型朊病毒 Mantoniella tinhauana virus 1 的基因组和生物地理学特征。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-11 DOI: 10.1111/1758-2229.70020

Elvira Rey Redondo, Shara Ka Kiu Leung, Charmaine Cheuk Man Yung

{"title":"Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1","authors":"Elvira Rey Redondo, Shara Ka Kiu Leung, Charmaine Cheuk Man Yung","doi":"10.1111/1758-2229.70020","DOIUrl":"10.1111/1758-2229.70020","url":null,"abstract":"Mamiellophyceae are a ubiquitous class of unicellular green algae in the global ocean. Their ecological importance is highlighted in studies focused on the prominent genera Micromonas, Ostreococcus, and Bathycoccus. Mamiellophyceae are susceptible to prasinoviruses, double-stranded DNA viruses belonging to the nucleocytoplasmic large DNA virus group. Our study represents the first isolation of a prasinovirus in the South China Sea and the only one to infect the globally distributed genus Mantoniella. We conducted a comparative analysis with previously identified viral relatives, encompassing morphological characteristics, host specificity, marker-based phylogenetic placement, and whole-genome sequence comparisons. Although it shares morphological and genetic similarities with established prasinoviruses, this novel virus showed distinct genetic traits, confining its infection to the species Mantoniella tinhauana. We also explored the global biogeography of this prasinovirus and its host by mapping metagenomic data and analysing their relationship with various environmental parameters. Our results demonstrate a pronounced link between the virus and its host, both found predominantly in higher latitudes in the surface ocean. By gaining an increased understanding of the relationships between viruses, hosts, and environments, researchers can better make predictions and potentially implement measures to mitigate the consequences of climate change on oceanic processes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial community response to novel and repeated disturbances 细菌群落对新干扰和重复干扰的反应

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-10 DOI: 10.1111/1758-2229.70022

Susannah Halbrook, William Wilber, Mary Elizabeth Barrow, Emily C. Farrer

{"title":"Bacterial community response to novel and repeated disturbances","authors":"Susannah Halbrook, William Wilber, Mary Elizabeth Barrow, Emily C. Farrer","doi":"10.1111/1758-2229.70022","DOIUrl":"10.1111/1758-2229.70022","url":null,"abstract":"Disturbance response and recovery are increasingly important in microbial ecology, as microbes may recover from disturbances differently than macro communities. Past disturbances can alter microbial community structure and their response to subsequent disturbance events, but it remains unclear if the same recovery patterns persist after long-term exposure to stress. Here, we compare bacterial community composition in a community that experienced 2 years of monthly salinity addition disturbances with a community that has not experienced salinity additions. We then track the response and recovery to an additional salinity addition based on past disturbance exposure. We tested the following hypotheses: first, communities with a repeated disturbance history will have a different community composition than communities without a disturbance history; second, communities exposed to repeated disturbances will undergo a different recovery trajectory than communities experiencing a novel disturbance. We find that repeated disturbances alter community composition and affect community response and recovery to a subsequent disturbance after 2 years, primarily through increased resistance. This work enhances our understanding of microbial temporal dynamics and suggests that novel disturbances may pose a threat to microbial community structure and function.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Pseudomonas strains isolated from Achnatherum inebrians on plant growth: A genomic perspective 从Achnatherum inebrians中分离的假单胞菌株对植物生长的影响：基因组视角

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-10 DOI: 10.1111/1758-2229.70011

Jinjin Liang, Bowen Liu, Michael J. Christensen, Chunjie Li, Xingxu Zhang, Zhibiao Nan

{"title":"The effects of Pseudomonas strains isolated from Achnatherum inebrians on plant growth: A genomic perspective","authors":"Jinjin Liang, Bowen Liu, Michael J. Christensen, Chunjie Li, Xingxu Zhang, Zhibiao Nan","doi":"10.1111/1758-2229.70011","DOIUrl":"10.1111/1758-2229.70011","url":null,"abstract":"Achnatherum inebrians is a perennial grass widely distributed in northwest China. Nearly all wild A. inebrians plants are infected by Epichloë endophytes. In this study, bacteria from the phyllosphere were isolated from leaves of both endophyte-free and endophyte-infected A. inebrians and sequenced for identification. Pseudomonas, comprising 48.12% of the culturable bacterial communities, was the most dominant bacterial genus. Thirty-four strains from 12 Pseudomonas species were used to inoculate A. inebrians seeds and plants. Results indicated that Epichloë significantly increased the diversity and richness index of the phyllosphere. Pseudomonas Sp1, Sp3, Sp5 and Sp7 had a significantly positive effect on plant growth and photosynthesis, whereas Sp10, Sp11 and Sp12 had a significantly negative effect. Whole-genome and pan-genome analysis suggested that the variability in the effects of Pseudomonas on A. inebrians was related to differences in genome composition and genomic islands.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retention of microplastics by biofilms and their ingestion by protists in rivers 河流中生物膜对微塑料的截留以及原生动物对微塑料的摄取。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-09 DOI: 10.1111/1758-2229.70016

Leandra Hamann, Jennifer Werner, Felicia J. Haase, Massimo Thiel, Anja Scherwaß, Christian Laforsch, Martin G. J. Löder, Alexander Blanke, Hartmut Arndt

{"title":"Retention of microplastics by biofilms and their ingestion by protists in rivers","authors":"Leandra Hamann, Jennifer Werner, Felicia J. Haase, Massimo Thiel, Anja Scherwaß, Christian Laforsch, Martin G. J. Löder, Alexander Blanke, Hartmut Arndt","doi":"10.1111/1758-2229.70016","DOIUrl":"10.1111/1758-2229.70016","url":null,"abstract":"Microplastics (MPs) are released into the environment through human activities and are transported by rivers from land to sea. Biofilms, which are ubiquitous in aquatic ecosystems such as rivers, may play an essential role in the fate of MPs and their ingestion by biofilm protists. To assess this, biofilms were naturally grown on clay tiles in the River Rhine, Germany, and analysed in a combined field and laboratory study. Compared to the ambient river water, biofilms grown for 6, 12, and 18 months in the River Rhine contained up to 10 times more MPs. Between 70% and 78% of all MPs were smaller than 50 μm. In laboratory experiments, clay tiles covered with 1-month-old naturally grown biofilm retained 6–12 times more MPs than clay tiles without biofilm coverage. Furthermore, the ingestion of MPs of 6 and 10 μm by the ciliate Stentor coeruleus was confirmed, and a positive correlation between ingestion rates and ambient MP concentrations was found. The results are relevant for particle transport models in riverine systems, risk assessment of MPs regarding their distribution and fate in the aquatic environment, and the effects of MPs on micro- and macroorganisms.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-07 DOI: 10.1111/1758-2229.70017

Biljana Ječmenica, Sanja Duvnjak, Andrea Humski, Louie Thomas Taylor, Jelena Kralj, Fani Krstulović, Tajana Amšel Zelenika, Viktor Mašović, Luka Jurinović

{"title":"Age-related presence and genetic diversity of Campylobacter spp. in young and adult yellow-legged gulls (Larus michahellis) in Croatia","authors":"Biljana Ječmenica, Sanja Duvnjak, Andrea Humski, Louie Thomas Taylor, Jelena Kralj, Fani Krstulović, Tajana Amšel Zelenika, Viktor Mašović, Luka Jurinović","doi":"10.1111/1758-2229.70017","DOIUrl":"10.1111/1758-2229.70017","url":null,"abstract":"The epidemiology of Campylobacter species in wild birds is still poorly understood. This study describes the occurrence and genetic diversity of Campylobacter in adult and nestlings of yellow-legged gulls, highlighting differences between breeding locations. The gulls were captured in Croatia between 2021 and 2023. A cloacal swab was taken from each individual and tested for the presence of Campylobacter. Isolated Campylobacter species were genotyped using the multilocus sequence typing (MLST) method. A total of 1071 gulls were captured and sampled, of which 152 samples were identified as Campylobacter species, with Campylobacter jejuni (9.90%) being the most frequently isolated bacterium, followed by Campylobacter lari (3.36%) and Campylobacter coli (0.93%). Complete sequence type (ST) profiles were generated for 141 isolates: 100 C. jejuni, 33 C. lari, and 8 C. coli. A significant difference in the occurrence of positive Campylobacter species was found depending on the sampling sites, while both sampling site and age were significant for the occurrence of C. jejuni. Adults and nestlings showed high genetic diversity for C. jejuni and C. lari, and there were no significant differences between strains isolated from adults and nestlings or between sites, suggesting a high genotype flow in the studied gull population.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole cell affinity for 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in the marine bacterium Candidatus Pelagibacter st. HTCC7211 explains marine dissolved HMP concentrations 海洋细菌 Candidatus Pelagibacter st. HTCC7211 对 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) 的全细胞亲和力解释了海洋溶解 HMP 的浓度。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-04 DOI: 10.1111/1758-2229.70023

Elizabeth Brennan, Stephen Noell, Edward W. Davis II, Stephen J. Giovannoni, Christopher P. Suffridge

{"title":"Whole cell affinity for 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in the marine bacterium Candidatus Pelagibacter st. HTCC7211 explains marine dissolved HMP concentrations","authors":"Elizabeth Brennan, Stephen Noell, Edward W. Davis II, Stephen J. Giovannoni, Christopher P. Suffridge","doi":"10.1111/1758-2229.70023","DOIUrl":"10.1111/1758-2229.70023","url":null,"abstract":"Vitamin B1 is a universally required coenzyme in carbon metabolism. However, most marine microorganisms lack the complete biosynthetic pathway for this compound and must acquire thiamin, or precursor molecules, from the dissolved pool. The most common version of Vitamin B1 auxotrophy is for thiamin's pyrimidine precursor moiety, 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). Frequent HMP auxotrophy in plankton and vanishingly low dissolved concentrations (approximately 0.1–50 pM) suggest that high-affinity HMP uptake systems are responsible for maintaining low ambient HMP concentrations. We used tritium-labelled HMP to investigate HMP uptake mechanisms and kinetics in cell cultures of Candidatus Pelagibacter st. HTCC7211, a representative of the globally distributed and highly abundant SAR11 clade. A single protein, the sodium solute symporter ThiV, which is conserved across SAR11 genomes, is the likely candidate for HMP transport. Experimental evidence indicated transport specificity for HMP and mechanistically complex, high-affinity HMP uptake kinetics. Km values ranged from 9.5 pM to 1.2 nM and were dramatically lower when cells were supplied with a carbon source. These results suggest that HMP uptake in HTCC7211 is subject to complex regulation and point to a strategy for high-affinity uptake of this essential growth factor that can explain natural HMP levels in seawater.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between Pseudomonas aeruginosa and six opportunistic pathogens cover a broad spectrum from mutualism to antagonism 铜绿假单胞菌与六种机会性病原体之间的相互作用涵盖了从互作到拮抗的广泛范围。

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2024-10-02 DOI: 10.1111/1758-2229.70015

Clémentine Laffont, Tobias Wechsler, Rolf Kümmerli

{"title":"Interactions between Pseudomonas aeruginosa and six opportunistic pathogens cover a broad spectrum from mutualism to antagonism","authors":"Clémentine Laffont, Tobias Wechsler, Rolf Kümmerli","doi":"10.1111/1758-2229.70015","DOIUrl":"10.1111/1758-2229.70015","url":null,"abstract":"Bacterial infections often involve more than one pathogen. While it is well established that polymicrobial infections can impact disease outcomes, we know little about how pathogens interact and affect each other's behaviour and fitness. Here, we used a microscopy approach to explore interactions between Pseudomonas aeruginosa and six human opportunistic pathogens that often co-occur in polymicrobial infections: Acinetobacter baumannii, Burkholderia cenocepacia, Escherichia coli, Enterococcus faecium, Klebsiella pneumoniae, and Staphylococcus aureus. When following growing microcolonies on agarose pads over time, we observed a broad spectrum of species-specific ecological interactions, ranging from mutualism to antagonism. For example, P. aeruginosa engaged in a mutually beneficial interaction with E. faecium but suffered from antagonism by E. coli. While we found little evidence for active directional growth towards or away from cohabitants, we observed that some pathogens increased growth in double layers in response to competition and that physical forces due to fast colony expansion had a major impact on fitness. Overall, our work provides an atlas of pathogen interactions, highlighting the diversity of potential species dynamics that may occur in polymicrobial infections. We discuss possible mechanisms driving pathogen interactions and offer predictions of how the different ecological interactions could affect virulence.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0