Environmental Microbiology Reports最新文献_第6页

Metabolic Flux and Growth Profiling of Megasphaera cerevisiae for Medium-Chain Fatty Acid Synthesis 酵母中链脂肪酸合成的代谢通量和生长特征。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-24 DOI: 10.1111/1758-2229.70390

Wael Sabra, Sonia Villotti, Joachim Fensterle, An-Ping Zeng, Ahmed M. Haddad

{"title":"Metabolic Flux and Growth Profiling of Megasphaera cerevisiae for Medium-Chain Fatty Acid Synthesis","authors":"Wael Sabra, Sonia Villotti, Joachim Fensterle, An-Ping Zeng, Ahmed M. Haddad","doi":"10.1111/1758-2229.70390","DOIUrl":"10.1111/1758-2229.70390","url":null,"abstract":"Megasphaera cerevisiae is a well-known beer spoilage organism, capable of producing undesirable flavours and turbidity. Although the biosynthesis of medium-chain fatty acids (MCFAs) has been extensively studied in different Megasphaera species, the metabolic behaviour of M. cerevisiae in controlled environments remains largely unexplored. This study examines the MCFAs production from diverse substrates and reports flux analyses of core metabolism for the first time using a genome-scale model. The results suggest that acetate stimulates butyrate production but not caproic acid production. Butyrate supplementation, either alone or in combination with acetate, promoted CA synthesis. Lactate supplementation primarily led to the formation of propionic acid and acetic acid. The metabolic network model was manually curated and validated against the different experimental data. The metabolic flux results showed that butyrate production facilitated via the reverse β-oxidation (RBO) pathway, with a minor contribution from the fatty acid synthesis (FAS) pathway. Conversely, CA synthesis was mainly synthesised through the FAS pathway, irrespective of the substrate used. The pathway analyses results highlighted the critical role of hydrogen production in M. cerevisiae metabolism, particularly under conditions where lactate is utilised. Collectively, these findings offer novel insights into the metabolic versatility and pathway preferences of M. cerevisiae.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Distribution of Environmental Pseudomonas aeruginosa From Hospitals in Bangladesh Reveals Diverse Strain Families, Multidrug Resistance, and Biofilm Formation 孟加拉国医院环境铜绿假单胞菌的发生和分布揭示了不同菌株家族，多重耐药性和生物膜的形成。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-24 DOI: 10.1111/1758-2229.70391

S. M. Kador, Tanay Chakrovarty, Khandaker Adil, Md. Mustak Ahmed, Md. Mohsin Kobir, Md. Hasanuzzaman, Mousufa Akter, Tanzima Sharmim Taowhid, Md. Tanvir Islam, Ovinu Kibria Islam

{"title":"Occurrence and Distribution of Environmental Pseudomonas aeruginosa From Hospitals in Bangladesh Reveals Diverse Strain Families, Multidrug Resistance, and Biofilm Formation","authors":"S. M. Kador, Tanay Chakrovarty, Khandaker Adil, Md. Mustak Ahmed, Md. Mohsin Kobir, Md. Hasanuzzaman, Mousufa Akter, Tanzima Sharmim Taowhid, Md. Tanvir Islam, Ovinu Kibria Islam","doi":"10.1111/1758-2229.70391","DOIUrl":"10.1111/1758-2229.70391","url":null,"abstract":"Pseudomonas aeruginosa is a major nosocomial pathogen capable of developing multidrug resistance, forming biofilms, and disseminating antimicrobial resistance genes via mobile genetic elements. This study investigated the occurrence, antimicrobial resistance, lecB sequence diversity, and biofilm-forming capacity of environmental P. aeruginosa isolates from three hospitals in Jashore, Bangladesh. Among 231 environmental samples collected from 29 sites in high-risk hospital units, 36 isolates were confirmed by lecB gene sequencing. Sequence analysis classified 77.8% of isolates as PAO1-like and 22.2% as PA14-like, indicating genetic diversity. Biofilm formation was detected in 97.2% of isolates, with no significant difference between strain families. Phenotypic susceptibility testing revealed widespread multidrug resistance, with the highest resistance to ceftazidime (97.2%) and tetracycline (91.7%). Two operation theatre isolates (A40 and 2SW) exhibited extensive drug resistance within the tested antimicrobial panel. Polymerase chain reaction detected blaSHV (55.6%), floR (33.3%), aac(6′)-Ib (33.3%), blaTEM (27.8%), and blaOXA-48 (11.1%), while class 1 integrons occurred in 55.6% of isolates and were associated with floR and blaTEM. Whole-genome sequencing identified intrinsic resistance determinants and the mobile resistance genes tmexCD-toprJ and crpP. These findings highlight genetically diverse, multidrug-resistant, integron-positive, biofilm-forming P. aeruginosa in Bangladeshi hospital environments, underscoring the need for routine environmental surveillance and strengthened infection prevention.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidden Microbiota Inhabiting in Pollen Reserves of Honey Bee (Apis mellifera) From Amazonas Region Revealed by DNA Metabarcoding 利用DNA元条形码揭示亚马孙地区蜜蜂花粉储备中隐藏的微生物群。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-22 DOI: 10.1111/1758-2229.70392

Jessica C. Llaja, Johann E. Oyola, Jois V. Carrion, Fernando Chuquizuta, Martha S. Calderon, Danilo E. Bustamante

{"title":"Hidden Microbiota Inhabiting in Pollen Reserves of Honey Bee (Apis mellifera) From Amazonas Region Revealed by DNA Metabarcoding","authors":"Jessica C. Llaja, Johann E. Oyola, Jois V. Carrion, Fernando Chuquizuta, Martha S. Calderon, Danilo E. Bustamante","doi":"10.1111/1758-2229.70392","DOIUrl":"10.1111/1758-2229.70392","url":null,"abstract":"Pollen functions as a dynamic microbial habitat and the microbes living in pollen reserves play vital roles in pollinator health and nutrition. However, the microbiota composition of honeybee pollen reserves in biodiverse Neotropical regions remains largely unknown. This study provides the first comprehensive analysis of bacterial and fungal communities in honeybee pollen reserves across six ecosystems in the Amazonas region of Peru using high-throughput metabarcoding of the 16S rRNA gene and ITS2 markers. We found that ecosystem type is a primary driver of community structure, with bacteria and fungi responding differently to environmental changes. Despite high taxonomic heterogeneity and a limited number of shared core microbes, the main functions of these microbes were maintained, featuring enrichment of bacterial pathways involved in nutrient metabolism and saprotrophic fungal guilds. Lactobacillus and an unclassified Tremellomycetes fungus were dominant, yet their abundance varied with respect to floral resource diversity. The simplified Palm Swamp ecosystem showed significantly reduced microbial diversity, underscoring the vulnerability of these communities to habitat homogenization. Our results demonstrate that the pollen reserve microbiome is assembled through environmental filtering and pollinator-mediated selection, resulting in taxonomically flexible but functionally stable communities essential for hive processes. This work provides a foundation for understanding the microbial ecology of pollen in the Amazonas region.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13392498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographic Variability of Fish Carcass–Associated Microbial Communities 鱼类尸体相关微生物群落的地理变异。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-22 DOI: 10.1111/1758-2229.70389

Yasutake Kawamoto, Jotaro Urabe

引用次数: 0

Correction to “Phosphorus Acquisition Strategies Among Phytoplankton and Free-Living Bacterial Communities in the Baltic Proper” 更正“波罗的海地区浮游植物和自由生活细菌群落的磷获取策略”

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-17 DOI: 10.1111/1758-2229.70387

引用次数: 0

Correction to “Phosphorus Acquisition Strategies Among Phytoplankton and Free-Living Bacterial Communities in the Baltic Proper” 更正“波罗的海地区浮游植物和自由生活细菌群落的磷获取策略”

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-17 DOI: 10.1111/1758-2229.70387

引用次数: 0

In Situ Bacterioplankton Growth Partitioning by High-Resolution Metatranscriptomics 利用高分辨率超转录组学研究浮游细菌的原位生长分配。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-09 DOI: 10.1111/1758-2229.70385

J. Cesar Ignacio-Espinoza, Yuxuan Zou, Andrew Long, Shengwei Hou, David M. Needham, Jed A. Fuhrman

{"title":"In Situ Bacterioplankton Growth Partitioning by High-Resolution Metatranscriptomics","authors":"J. Cesar Ignacio-Espinoza, Yuxuan Zou, Andrew Long, Shengwei Hou, David M. Needham, Jed A. Fuhrman","doi":"10.1111/1758-2229.70385","DOIUrl":"10.1111/1758-2229.70385","url":null,"abstract":"Microbial communities are fundamental to marine trophic webs, elemental cycling and geochemical transformations. Yet we lack high phylogenetic resolution measurements or good indicators of how fast different taxa are actively growing in situ. Here, we use high temporal resolution transcriptomics to phylogenetically disentangle an index of growth before and after a short spring phytoplankton bloom, by quantifying the phylogenetic distribution of the expression of ftsZ, a gene encoding for a protein involved in cell division. We interpret the relative abundances of ftsZ transcripts as a general indicator of how growth was distributed amongst taxa. This expression was also compared to RNase P to estimate how each organism's transcriptional resources were allocated to replication versus other functions. During the time-series, we observed two distinct profiles: prior to and several days after the bloom, ftsZ expression was dominated by Synechococcales, Pelagibacterales and picoeukaryotes, and by Rhodobacterales, SAR92 and SAR86 as the bloom declined. Whilst similar successional patterns have been observed previously, our dataset extends these observations by resolving transcriptional and replication-associated activity at high temporal resolution, enabling the detection of disproportionate contributions during bloom development and turnover. Our approach is scalable and will inform conceptual and mechanistic models of planktonic food webs.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking Biocrust Architecture and Dispersal: Reproductive Ecology of Lichens of the Grit Crust in the Coastal Atacama Desert 连接生物外壳结构和扩散：阿塔卡马沙漠沿海沙砾壳地衣的生殖生态学。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-05 DOI: 10.1111/1758-2229.70384

Lina Werner, Janina Koziol, Patrick Jung

{"title":"Linking Biocrust Architecture and Dispersal: Reproductive Ecology of Lichens of the Grit Crust in the Coastal Atacama Desert","authors":"Lina Werner, Janina Koziol, Patrick Jung","doi":"10.1111/1758-2229.70384","DOIUrl":"10.1111/1758-2229.70384","url":null,"abstract":"Biological soil crusts are essential components of arid ecosystems, yet the establishment and dispersal of lichen-dominated crusts remain poorly understood. In the coastal Atacama Desert, a unique biocrust type—the grit crust—is formed by minute chlorolichens colonising mobile quartz particles. We investigated the developmental stages and dispersal mechanisms of these micro-lichens using high-resolution digital microscopy, scanning electron microscopy and micro-manipulative direct PCR. Microscopic structures on individual quartz grains included melanised fungal micro-colonies, exploratory hyphal networks, free-living green algal colonies and developing lichen thalli. Molecular analyses identified mycobionts of the Caliciaceae alongside photobionts of the green algal genus Trebouxia, occurring in both lichenised and non-lichenised states. The presence of free-living algal cells on grit surfaces suggests that unassociated photobionts function as environmental reservoirs priming symbiosis formation. Our findings support a sequential assembly model in which airborne fungal spores colonise mineral substrates before encountering compatible photobionts. Multiple dispersal vectors—including fungal spores, lichen fragments and algal cells carried by wind and dust—confer high colonisation and recovery potential to grit crust communities, likely driving the rapid turnover, spatial heterogeneity and productivity characteristic of this exceptional lichen-dominated desert biocrust.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148389451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Determining the Number of Mold Fungi in the Air—Current Trends 气流趋势中霉菌真菌数量的测定方法。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-07-05 DOI: 10.1111/1758-2229.70386

Juhi Mishra, Wioletta Przystas

{"title":"Methods for Determining the Number of Mold Fungi in the Air—Current Trends","authors":"Juhi Mishra, Wioletta Przystas","doi":"10.1111/1758-2229.70386","DOIUrl":"10.1111/1758-2229.70386","url":null,"abstract":"Indoor fungal contamination poses significant health concerns due to the diversity and dynamic behaviour of airborne spores. Accurate quantification of indoor fungal bioaerosols remains challenging because of variability in sampling efficiency, particle-size resolution, and analytical approaches. This review critically evaluates commonly used physical sampling methods, including Andersen cascade impactors, Coriolis μ liquid cyclones, sedimentation techniques, and filter-based air sampling, with emphasis on volumetric accuracy, reproducibility, and compatibility with downstream molecular analysis. The processes underlying spore aerosolization in indoor environments and their implications for air quality and occupant exposure are discussed. Indoor fungi such as Aspergillus, Penicillium, Cladosporium, and Rhizopus proliferate under high humidity and inadequate ventilation, particularly within damp materials and HVAC systems. Despite technological advances, significant gaps persist in methodological standardization, harmonization of exposure metrics, and integration of indoor–outdoor fungal dynamics. Such heterogeneity limits cross-study comparability and constrains exposure assessment. By systematically comparing available methods and their optimal applications, this review highlights the need for integrated and standardized strategies to improve fungal bioaerosol quantification and support evidence-based indoor air quality management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70386","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148389517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flower-Doped Carbon Quantum Dots Improve Ceratobasidium sp. Growth Efficiency: A Green Nanotechnology Strategy for Fungal Applications 花掺杂碳量子点提高白角霉生长效率：一种用于真菌应用的绿色纳米技术策略

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-30 DOI: 10.1111/1758-2229.70382

Bengisu Sentürk, Erdi Can Aytar, Major Mabuza, Idara Asuquo Okon, Mika Sillanpää, Yasemin Özdener Kömpe

{"title":"Flower-Doped Carbon Quantum Dots Improve Ceratobasidium sp. Growth Efficiency: A Green Nanotechnology Strategy for Fungal Applications","authors":"Bengisu Sentürk, Erdi Can Aytar, Major Mabuza, Idara Asuquo Okon, Mika Sillanpää, Yasemin Özdener Kömpe","doi":"10.1111/1758-2229.70382","DOIUrl":"https://doi.org/10.1111/1758-2229.70382","url":null,"abstract":"This study investigates the characteristics of AL (Anacamptis laxiflora flower)-doped carbon quantum dots (AL-CQDs), synthesised via a 7-h pyrolysis process and their effects on the growth of the Ceratobasidium sp. (AL5) fungus. AL-CQDs exhibited strong ultraviolet–visible absorption and a consistent spherical morphology. High-resolution transmission electron microscopy (HR-TEM) analysis revealed that the particles were homogeneously distributed, predominantly spherical or quasi-spherical in shape and ranged in size from 45 to 110 nm. In addition, the HR-TEM images showed localised ordered domains with an average interplanar spacing of approximately 0.113 nm, indicating the presence of graphitic or crystalline-like regions within the carbon dots. Fungal growth experiments demonstrated that nutrient media supplemented with CQDs significantly increased the growth rate and decreased hyphal diameter, suggesting more efficient nutrient uptake. The medium containing 2 g of CQDs promoted the fastest growth, reaching maximum levels by Day 5, whereas the 1 g CQD medium resulted in a more compact cell structure and steady growth. These findings suggest that AL-CQDs enhance fungal growth and may have promising applications in fungal biotechnology and nanobiotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0