Environmental Microbiology Reports最新文献_第7页

Evaluating Bacteriophage Impact on Vibrio Composition in the Gut of Broad-Nosed Pipefish (Syngnathus typhle) 评估噬菌体对宽鼻管鱼肠道弧菌组成的影响。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-07 DOI: 10.1111/1758-2229.70125

Jamie Parker, Silke-Mareike Marten, Jelena Rajkov, Franziska I. Theising, Arseny Dubin, Olivia Roth

{"title":"Evaluating Bacteriophage Impact on Vibrio Composition in the Gut of Broad-Nosed Pipefish (Syngnathus typhle)","authors":"Jamie Parker, Silke-Mareike Marten, Jelena Rajkov, Franziska I. Theising, Arseny Dubin, Olivia Roth","doi":"10.1111/1758-2229.70125","DOIUrl":"10.1111/1758-2229.70125","url":null,"abstract":"Bacteriophages play a crucial role in shaping microbial community dynamics in marine systems and have the potential to stimulate surges in pathogenic bacteria, facilitating disease outbreaks. Notwithstanding, bacteriophages also serve as valuable biocontrol agents, underscoring their huge potential for aquaculture therapy treatments. Empirical insights into the intricate tripartite interplay upon exposure to a virulent bacterium, its specific phages, and the host gut microbiome could improve our understanding of how bacteria–phage interactions behave in a natural microbial system. This investigation assessed the influence of a virulent Vibrio alginolyticus (K01M1) infection, in tandem with lytic (фSt2) and filamentous (фK04M1) Vibrio-specific phages, on the broad-nosed pipefish (Syngnathus typhle) gut microbiome using 16S rRNA amplicon sequencing and non-intrusive gastric swabbing. The pipefish microbiome structure was not impacted by Vibrio and phage introductions, while the different infection regimes shaped Vibrio-specific dynamics. In the filamentous phage and Vibrio-only treatments, V. alginolyticus abundances spiked 12 h post-ingestion. In contrast, V. alginolyticus numbers in the lytic phage and control treatment were significantly reduced, suggesting phage activity and specific elimination of the introduced bacteria. Assisted by relative true-gut tissue samples, a newly implemented non-intrusive swabbing method was successful at discerning the activity of two contrasting phages and supports previous work that encourages the use of фSt2 in bacteriophage treatments. Identifying Vibrio-specific phages with similar positive characteristics could be beneficial for the aquaculture trade, which is currently heavily impacted by the antibiotic crisis.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling Intrinsic and Extrinsic Factors Shaping the Rich Communities on Lizard Skin 揭示塑造蜥蜴皮肤上丰富群落的内在和外在因素。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-06 DOI: 10.1111/1758-2229.70172

Chava L. Weitzman, Kimberley Day, Karen Gibb, Gregory P. Brown, Angga Rachmansah, Keith Christian

{"title":"Unravelling Intrinsic and Extrinsic Factors Shaping the Rich Communities on Lizard Skin","authors":"Chava L. Weitzman, Kimberley Day, Karen Gibb, Gregory P. Brown, Angga Rachmansah, Keith Christian","doi":"10.1111/1758-2229.70172","DOIUrl":"10.1111/1758-2229.70172","url":null,"abstract":"Geckos have high skin bacterial diversity, even though gecko skin has antimicrobial, self-cleaning properties. To gain a better understanding of environment–animal–microbiome interactions in these reptiles, we investigated skin bacteria on seven northern Australian gecko species from five sites and two seasons (n = 234) and found support for our hypotheses of divergent communities between species, sites and seasons. Despite that support, predictor variables had low explanatory power, which increased when focusing within a site or species, explaining up to 40% and 27% of the variation among samples at a site or on a species found in multiple sites, respectively. Weather explained even less variation, as temperature and rainfall did not account for site and season differences. Low explanatory power of these variables indicates that additional factors, or stochasticity, explain much of the bacterial assemblage on geckos. Next, research is needed to determine if these low-biomass communities represent living symbionts. If so, assessing functional similarities, rather than taxonomic profiling, would clarify if bacterial communities have interactive roles with gecko hosts or represent short-lived environmental hitch-hikers and relic DNA.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic and Functional Features of Surface to Deep-Sea Prokaryotic Communities in the Eastern North Pacific Ocean 北太平洋东部表层至深海原核生物群落的分类与功能特征。

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-06 DOI: 10.1111/1758-2229.70170

Daniele De Corte, Leon Dlugosch, Abhishek Srivastava, Meinhard Simon, Dennis A. Hansell, Sarah Bercovici, Monica Orellana

{"title":"Taxonomic and Functional Features of Surface to Deep-Sea Prokaryotic Communities in the Eastern North Pacific Ocean","authors":"Daniele De Corte, Leon Dlugosch, Abhishek Srivastava, Meinhard Simon, Dennis A. Hansell, Sarah Bercovici, Monica Orellana","doi":"10.1111/1758-2229.70170","DOIUrl":"10.1111/1758-2229.70170","url":null,"abstract":"Biogeochemical cycles in the ocean are strongly influenced by microbial activity, which affects nutrient and organic matter cycling. These processes, influenced by factors such as temperature, salinity, density and inorganic nutrients, drive the vertical stratification of microbial communities, which subsequently influence the chemistry at different depth layers. Sequencing technology has expanded our understanding of oceanic prokaryotic communities' taxonomic and functional potential. However, there is limited information on how these communities vary across gradients. In this study, we conducted metagenomic analyses on samples from the eastern North Pacific, collected across a longitudinal transect around 45°N and throughout the entire water column. We assessed taxonomic and functional classification, focusing on the roles of prokaryotic communities in biogeochemical cycling. Our results revealed that the surface community was dominated by the SAR11 clade, followed by Flavobacterales and Rhodobacterales. The deep layers harboured a more diverse community, where Thaumarchaeota accounted for the most significant proportion. This clear taxonomic stratification led to variations in the communities' functional capabilities across different depth layers. Photosynthesis and heterotrophy dominated the surface layers, whereas the deeper layers exhibited a mix of metabolic features, allowing organisms to potentially utilise both inorganic and organic carbon sources.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual Plastoquinones in Non-Phototrophic Nitrifying Bacteria 非光养硝化细菌中不寻常的质体醌

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-05 DOI: 10.1111/1758-2229.70174

Nicole J. Bale, Hayato Fujimura, Petra Pjevac, Michel Koenen, Hikaru Ikeda, Satohiro Itagaki, Yojiro Yamamoto, Johanna Palmetzhofer, Christopher J. Sedlacek, Hayk Palabikyan, Jaap S. Sinninghe Damsté, Michael Wagner, Hiroshi Shiigi, Holger Daims

{"title":"Unusual Plastoquinones in Non-Phototrophic Nitrifying Bacteria","authors":"Nicole J. Bale, Hayato Fujimura, Petra Pjevac, Michel Koenen, Hikaru Ikeda, Satohiro Itagaki, Yojiro Yamamoto, Johanna Palmetzhofer, Christopher J. Sedlacek, Hayk Palabikyan, Jaap S. Sinninghe Damsté, Michael Wagner, Hiroshi Shiigi, Holger Daims","doi":"10.1111/1758-2229.70174","DOIUrl":"https://doi.org/10.1111/1758-2229.70174","url":null,"abstract":"Isoprenoid quinones are important compounds in most organisms. They are essential in electron and proton transport in respiratory and photosynthetic electron transport chains, and additional functions include oxidative stress defence. The biologically most relevant quinones are naphthoquinones including menaquinone and benzoquinones including ubiquinone and plastoquinone. They differ in their polar headgroup structures, physicochemical properties, and distribution among organisms. Menaquinone is the most widespread quinone in prokaryotes, ubiquinone occurs only in bacteria of the phylum Pseudomonadota and eukaryotes, and plastoquinone exists in phototrophic Cyanobacteria and plants. We found that chemolithoautotrophic nitrifying bacteria of the genus Nitrospira (phylum Nitrospirota) exclusively possess unusual methyl-plastoquinones with a standard redox potential below that of canonical plastoquinone and ubiquinone but above menaquinone, suggesting functional roles in reverse electron transport, ammonia oxidation, alternative energy metabolisms, and oxidative stress mitigation. This extends the known diversity of quinones and suggests that plastoquinone derivatives are essential in ecologically important, non-phototrophic bacteria.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual Plastoquinones in Non-Phototrophic Nitrifying Bacteria 非光养硝化细菌中不寻常的质体醌

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-05 DOI: 10.1111/1758-2229.70174

Nicole J. Bale, Hayato Fujimura, Petra Pjevac, Michel Koenen, Hikaru Ikeda, Satohiro Itagaki, Yojiro Yamamoto, Johanna Palmetzhofer, Christopher J. Sedlacek, Hayk Palabikyan, Jaap S. Sinninghe Damsté, Michael Wagner, Hiroshi Shiigi, Holger Daims

{"title":"Unusual Plastoquinones in Non-Phototrophic Nitrifying Bacteria","authors":"Nicole J. Bale, Hayato Fujimura, Petra Pjevac, Michel Koenen, Hikaru Ikeda, Satohiro Itagaki, Yojiro Yamamoto, Johanna Palmetzhofer, Christopher J. Sedlacek, Hayk Palabikyan, Jaap S. Sinninghe Damsté, Michael Wagner, Hiroshi Shiigi, Holger Daims","doi":"10.1111/1758-2229.70174","DOIUrl":"10.1111/1758-2229.70174","url":null,"abstract":"Isoprenoid quinones are important compounds in most organisms. They are essential in electron and proton transport in respiratory and photosynthetic electron transport chains, and additional functions include oxidative stress defence. The biologically most relevant quinones are naphthoquinones including menaquinone and benzoquinones including ubiquinone and plastoquinone. They differ in their polar headgroup structures, physicochemical properties, and distribution among organisms. Menaquinone is the most widespread quinone in prokaryotes, ubiquinone occurs only in bacteria of the phylum Pseudomonadota and eukaryotes, and plastoquinone exists in phototrophic Cyanobacteria and plants. We found that chemolithoautotrophic nitrifying bacteria of the genus Nitrospira (phylum Nitrospirota) exclusively possess unusual methyl-plastoquinones with a standard redox potential below that of canonical plastoquinone and ubiquinone but above menaquinone, suggesting functional roles in reverse electron transport, ammonia oxidation, alternative energy metabolisms, and oxidative stress mitigation. This extends the known diversity of quinones and suggests that plastoquinone derivatives are essential in ecologically important, non-phototrophic bacteria.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae) 塑料污染对疟媒阿拉伯按蚊肠道菌群的影响（双翅目：库蚊科）

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-03 DOI: 10.1111/1758-2229.70169

Shristi Misser, Chia-Yu Chen, Arshad Ismail, Shüné V. Oliver

{"title":"The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae)","authors":"Shristi Misser, Chia-Yu Chen, Arshad Ismail, Shüné V. Oliver","doi":"10.1111/1758-2229.70169","DOIUrl":"https://doi.org/10.1111/1758-2229.70169","url":null,"abstract":"Plastic pollution is prevalent in water bodies. However, most studies on plastic pollution focus on marine environments, with limited knowledge about its impact on freshwater ecosystems. This paucity of information extends to the effect on aquatic insects, with little reported data on the effect of plastic on malaria vectors. This is concerning as microplastics are reported to perturb the gut microbiota of culicine mosquitoes. This study examines how larval exposure to degraded plastic, plastic additives (phthalic acid, Bisphenol-A) and latex beads affects the gut microbiota of adult Anopheles arabiensis, with a comparison of the insecticide-unselected (SENN) and insecticide-selected (SENN-DDT) strains. The larval exposure had a minimal effect on alpha-diversity, but each plastic stressor altered beta-diversity in a non-strain–specific manner. Plastic-treated SENN showed an increase in unique bacterial genera. In contrast, untreated SENN-DDT displayed the highest abundance of unique genera, suggesting gut bacteria may play a role in mitigating the effect of plastic exposure in unselected strains. Additionally, larval plastic exposure increased bacteria associated with plastic degradation and pesticide metabolism. Although there was no significant change in Plasmodium-protective bacterial genera, inflammation-associated bacterial genera increased in both strains after treatment, suggesting potential immune modulation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae) 塑料污染对疟媒阿拉伯按蚊肠道菌群的影响（双翅目：库蚊科）

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-03 DOI: 10.1111/1758-2229.70169

Shristi Misser, Chia-Yu Chen, Arshad Ismail, Shüné V. Oliver

{"title":"The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae)","authors":"Shristi Misser, Chia-Yu Chen, Arshad Ismail, Shüné V. Oliver","doi":"10.1111/1758-2229.70169","DOIUrl":"10.1111/1758-2229.70169","url":null,"abstract":"Plastic pollution is prevalent in water bodies. However, most studies on plastic pollution focus on marine environments, with limited knowledge about its impact on freshwater ecosystems. This paucity of information extends to the effect on aquatic insects, with little reported data on the effect of plastic on malaria vectors. This is concerning as microplastics are reported to perturb the gut microbiota of culicine mosquitoes. This study examines how larval exposure to degraded plastic, plastic additives (phthalic acid, Bisphenol-A) and latex beads affects the gut microbiota of adult Anopheles arabiensis, with a comparison of the insecticide-unselected (SENN) and insecticide-selected (SENN-DDT) strains. The larval exposure had a minimal effect on alpha-diversity, but each plastic stressor altered beta-diversity in a non-strain–specific manner. Plastic-treated SENN showed an increase in unique bacterial genera. In contrast, untreated SENN-DDT displayed the highest abundance of unique genera, suggesting gut bacteria may play a role in mitigating the effect of plastic exposure in unselected strains. Additionally, larval plastic exposure increased bacteria associated with plastic degradation and pesticide metabolism. Although there was no significant change in Plasmodium-protective bacterial genera, inflammation-associated bacterial genera increased in both strains after treatment, suggesting potential immune modulation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the Top-Down Control of Planktonic Bacteria in Response to Nutrient Addition and Warming in the Red Sea 红海浮游细菌对营养物添加和变暖的自上而下控制的变化

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-02 DOI: 10.1111/1758-2229.70166

Eman I. Sabbagh, Najwa Al-Otaibi, Maria Ll. Calleja, Daniele Daffonchio, Xosé Anxelu G. Morán

{"title":"Changes in the Top-Down Control of Planktonic Bacteria in Response to Nutrient Addition and Warming in the Red Sea","authors":"Eman I. Sabbagh, Najwa Al-Otaibi, Maria Ll. Calleja, Daniele Daffonchio, Xosé Anxelu G. Morán","doi":"10.1111/1758-2229.70166","DOIUrl":"10.1111/1758-2229.70166","url":null,"abstract":"Eutrophication and warming impacts on marine bacterioplankton and their top-down controls (protistan grazers and viruses) are still little known. Here, we evaluated the seasonal variability of the joint impact of nutrient addition and temperature on the abundance of bacterioplankton, heterotrophic nanoflagellates (HNFs) and viruses in Red Sea coastal waters. We conducted four microcosm experiments in which samples were either incubated as such (control, C) or amended with phosphate and nitrate (inorganic, I), glucose (organic, O) or both types of nutrients (mixed, M). Each nutrient treatment was incubated at three temperatures spanning 6°C around ambient values (23°C–33°C). Microbial response ratios (RR, the ratio between the maximum abundance in each nutrient amendment treatment relative to the maximum abundance in the C treatment) were variable, with the most noticeable increases found in the I and M treatments, suggesting an effect mediated by increased primary production. Bacterioplankton showed weak responses to warming, but the RRs of HNFs and viruses in the I treatment tended to increase at higher temperatures. The response of HNFs to the increase in prey was stronger than that of viruses. Our results also suggest that the coupling between heterotrophic bacteria and HNFs will likely increase with future warming.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the Top-Down Control of Planktonic Bacteria in Response to Nutrient Addition and Warming in the Red Sea 红海浮游细菌对营养物添加和变暖的自上而下控制的变化

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-02 DOI: 10.1111/1758-2229.70166

Eman I. Sabbagh, Najwa Al-Otaibi, Maria Ll. Calleja, Daniele Daffonchio, Xosé Anxelu G. Morán

{"title":"Changes in the Top-Down Control of Planktonic Bacteria in Response to Nutrient Addition and Warming in the Red Sea","authors":"Eman I. Sabbagh, Najwa Al-Otaibi, Maria Ll. Calleja, Daniele Daffonchio, Xosé Anxelu G. Morán","doi":"10.1111/1758-2229.70166","DOIUrl":"10.1111/1758-2229.70166","url":null,"abstract":"Eutrophication and warming impacts on marine bacterioplankton and their top-down controls (protistan grazers and viruses) are still little known. Here, we evaluated the seasonal variability of the joint impact of nutrient addition and temperature on the abundance of bacterioplankton, heterotrophic nanoflagellates (HNFs) and viruses in Red Sea coastal waters. We conducted four microcosm experiments in which samples were either incubated as such (control, C) or amended with phosphate and nitrate (inorganic, I), glucose (organic, O) or both types of nutrients (mixed, M). Each nutrient treatment was incubated at three temperatures spanning 6°C around ambient values (23°C–33°C). Microbial response ratios (RR, the ratio between the maximum abundance in each nutrient amendment treatment relative to the maximum abundance in the C treatment) were variable, with the most noticeable increases found in the I and M treatments, suggesting an effect mediated by increased primary production. Bacterioplankton showed weak responses to warming, but the RRs of HNFs and viruses in the I treatment tended to increase at higher temperatures. The response of HNFs to the increase in prey was stronger than that of viruses. Our results also suggest that the coupling between heterotrophic bacteria and HNFs will likely increase with future warming.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Microbiome of Tyrophagus Putrescentiae Culture and Its Changes in Manipulative Experiments 腐酪菌培养环境微生物组及其操作实验变化

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-08-01 DOI: 10.1111/1758-2229.70142

Jan Hubert, Bruno Sopko, Eliza Głowska-Patyniak

{"title":"Environmental Microbiome of Tyrophagus Putrescentiae Culture and Its Changes in Manipulative Experiments","authors":"Jan Hubert, Bruno Sopko, Eliza Głowska-Patyniak","doi":"10.1111/1758-2229.70142","DOIUrl":"https://doi.org/10.1111/1758-2229.70142","url":null,"abstract":"Storage mites consume stored products in interaction with environmental microorganisms, resulting in the destruction of infested food and providing specific odours. Here we simulated the effect of mite grazing on oat flakes. Spent growth medium (SPGM) was obtained from seven mite cultures and mixed with oat flakes as the source of faeces and microbes. SPGM-treated diets were offered to 4 mite cultures. The microbiomes were analysed using sequencing of V4_16S_DNA. Mite growth tests, food preferences, and microbiome changes were observed in correlation with SPGM type and mite cultures. The microbiome consisted of 41 OTUs belonging to mite-associated bacteria and faeces bacteria. The composition of the microbiome depends more on the source of SPGM than on mite culture. The SPGM diet accelerated mite population growth and influenced mite food choice, although the effect was dependent on both types of SPGM and mite culture. Kocuria, Brevibacterium, Virgibacillus, and Staphylococcus profiles in SPGM added into diets showed positive correlations to mite population growth. The Kocuria profile in the bodies of mites was positively correlated with mite population growth. The results showed that mites are influenced by SPGM-treated diets, and mite feeding influences the environmental microbiome. The most beneficial was the mite interaction with Kocuria.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0