Environmental Microbiology Reports最新文献_第7页

Accumulation of Histidine Reduce the Susceptibility of Vibrio splendidus Persister Cells to Tetracycline 组氨酸的积累降低了脾弧菌对四环素的敏感性。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-30 DOI: 10.1111/1758-2229.70383

Yanan Li, Xinnan Pan, Jiayao Chen, Hong Ding, Yina Shao

{"title":"Accumulation of Histidine Reduce the Susceptibility of Vibrio splendidus Persister Cells to Tetracycline","authors":"Yanan Li, Xinnan Pan, Jiayao Chen, Hong Ding, Yina Shao","doi":"10.1111/1758-2229.70383","DOIUrl":"10.1111/1758-2229.70383","url":null,"abstract":"Vibrio splendidus is an opportunistic pathogen widely distributed in marine environments, animal tissues and seabed sediments. It can infect various marine animals, resulting in high mortality and substantial economic losses. Exposure to high concentrations of tetracycline induces V. splendidus to form persister cells, which exhibit tolerance to multiple classes of antibiotics and pose a serious threat to aquaculture by promoting disease outbreaks. Previous studies have suggested that metabolic regulation is a key mechanism for maintaining the dormant and low-energy state of persister cells. In this study, we found that the accumulation of histidine-derived metabolites significantly reduced the susceptibility of V. splendidus to tetracycline. Transcriptomic analysis revealed a marked upregulation of genes involved in histidine metabolism and exogenous supplementation of histidine significantly increased the proportion of persister cells, suggesting that histidine plays a promotive role in persister formation. Furthermore, histidine enhanced the membrane potential and upregulated the expression of the efflux pump gene tolC, thereby contributing to the formation of tetracycline-induced persister cells. These findings reveal a previously unrecognized role of histidine metabolism in antibiotic tolerance and provide a theoretical basis for understanding persister cell formation in V. splendidus.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70383","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flower-Doped Carbon Quantum Dots Improve Ceratobasidium sp. Growth Efficiency: A Green Nanotechnology Strategy for Fungal Applications 花掺杂碳量子点提高白角霉生长效率：一种用于真菌应用的绿色纳米技术策略

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-30 DOI: 10.1111/1758-2229.70382

Bengisu Sentürk, Erdi Can Aytar, Major Mabuza, Idara Asuquo Okon, Mika Sillanpää, Yasemin Özdener Kömpe

{"title":"Flower-Doped Carbon Quantum Dots Improve Ceratobasidium sp. Growth Efficiency: A Green Nanotechnology Strategy for Fungal Applications","authors":"Bengisu Sentürk, Erdi Can Aytar, Major Mabuza, Idara Asuquo Okon, Mika Sillanpää, Yasemin Özdener Kömpe","doi":"10.1111/1758-2229.70382","DOIUrl":"https://doi.org/10.1111/1758-2229.70382","url":null,"abstract":"This study investigates the characteristics of AL (Anacamptis laxiflora flower)-doped carbon quantum dots (AL-CQDs), synthesised via a 7-h pyrolysis process and their effects on the growth of the Ceratobasidium sp. (AL5) fungus. AL-CQDs exhibited strong ultraviolet–visible absorption and a consistent spherical morphology. High-resolution transmission electron microscopy (HR-TEM) analysis revealed that the particles were homogeneously distributed, predominantly spherical or quasi-spherical in shape and ranged in size from 45 to 110 nm. In addition, the HR-TEM images showed localised ordered domains with an average interplanar spacing of approximately 0.113 nm, indicating the presence of graphitic or crystalline-like regions within the carbon dots. Fungal growth experiments demonstrated that nutrient media supplemented with CQDs significantly increased the growth rate and decreased hyphal diameter, suggesting more efficient nutrient uptake. The medium containing 2 g of CQDs promoted the fastest growth, reaching maximum levels by Day 5, whereas the 1 g CQD medium resulted in a more compact cell structure and steady growth. These findings suggest that AL-CQDs enhance fungal growth and may have promising applications in fungal biotechnology and nanobiotechnology.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-Specific Optimisation and Environmental Regulation of Biofilm Formation in Enterobacter cloacae: Inhibitory Role of Glucose in Biofilm Development. 阴沟肠杆菌生物膜形成的物种特异性优化和环境调控：葡萄糖在生物膜发育中的抑制作用。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70347

Srishti Singh, Virendra Bahadur Yadav, Alok Kumar Singh, Gopal Nath, Sasmita Chand, Nirmalendu Sekhar Mishra, Jagdeep Kumar Nayak

{"title":"Species-Specific Optimisation and Environmental Regulation of Biofilm Formation in Enterobacter cloacae: Inhibitory Role of Glucose in Biofilm Development.","authors":"Srishti Singh, Virendra Bahadur Yadav, Alok Kumar Singh, Gopal Nath, Sasmita Chand, Nirmalendu Sekhar Mishra, Jagdeep Kumar Nayak","doi":"10.1111/1758-2229.70347","DOIUrl":"10.1111/1758-2229.70347","url":null,"abstract":"Bacterial biofilms are prevalent in clinical environments, contributing to persistent infections associated with medical devices. Enterobacter cloacae forms biofilms on nonliving surfaces, leading to drug-resistant, recurrent infections that are difficult to treat. Biofilm development in Enterobacter species, including E. cloacae, occurs through five stages: reversible attachment, irreversible attachment, microcolony formation, maturation and dispersal. Initial attachment is mediated by adhesins, including fimbriae and lipopolysaccharides, which interact with surfaces. This is followed by secretion of an extracellular polymeric substance matrix composed of polysaccharides, extracellular DNA and proteins, providing stability and protection. This study aimed to establish a standardised in vitro 0.5% crystal violet staining method to quantify biofilm production in E. cloacae isolates and classify isolates by biofilm-forming capacity. Biofilm was quantified by optical density at 570-600 nm. A 96-well microtiter plate assay quantified biofilm formation in 40 E. cloacae strains collected between July 2021 and April 2023. Growth conditions were optimised, including culture media, fixation techniques and additive concentrations of glucose and sodium chloride. Brain heart infusion broth was optimal, and heat fixation was superior; glucose had no effect, whereas 1%-2% sodium chloride enhanced biofilm production. These findings improve understanding of environmental regulation of biofilm formation and microbial persistence across habitats.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70347"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148003964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Evaluation of Thymus serpyllum Essential Oil Against Paracoccus marcusii IBR3: A Potential Natural Protection Strategy for Historic Mural Paintings. 麝香精油抗马库副球菌IBR3的体外评价：一种潜在的历史壁画自然保护策略

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70368

Michele Narduzzi, Claudia Pelosi, Vittorio Vinciguerra, Chiara Antonelli, Anna Maria Vettraino

{"title":"In Vitro Evaluation of Thymus serpyllum Essential Oil Against Paracoccus marcusii IBR3: A Potential Natural Protection Strategy for Historic Mural Paintings.","authors":"Michele Narduzzi, Claudia Pelosi, Vittorio Vinciguerra, Chiara Antonelli, Anna Maria Vettraino","doi":"10.1111/1758-2229.70368","DOIUrl":"10.1111/1758-2229.70368","url":null,"abstract":"Historic mural paintings are particularly vulnerable to biodeterioration caused by microbial colonization, which can lead to the degradation of the organic and inorganic components of wall surfaces. This study presents, to the best of our knowledge, the first identification of the Paracoccus marcusii IBR3 strain on a 19th-century mural painting from a private Italian villa. In vitro analyses revealed that strain IBR3 exhibits significant enzymatic activities, such as casein hydrolysis and calcium carbonate dissolution. These activities implicate the strain in the degradation of proteinaceous binders and mineral substrates, which are typical of mural artwork. As part of an environmentally sustainable conservation strategy, the antimicrobial activity of Thymus serpyllum essential oil was evaluated against strain IBR3 using in vitro tests, membrane integrity assays, and ATR-FTIR spectroscopy. The essential oil, chemically characterized by GC-MS, demonstrated promising biocidal efficacy. These findings suggest that T. serpyllum essential oil could serve as a non-toxic alternative for preserving and maintaining culturally significant wall paintings.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70368"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13239984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148051560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Survey of Carbon Monoxide Dehydrogenases Reveals Their Widespread Distribution in Marine Habitats. 一氧化碳脱氢酶的基因组调查揭示了它们在海洋栖息地的广泛分布。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70375

Nipa Chongdar, Anand Goyal, Samir R Damare

{"title":"Genomic Survey of Carbon Monoxide Dehydrogenases Reveals Their Widespread Distribution in Marine Habitats.","authors":"Nipa Chongdar, Anand Goyal, Samir R Damare","doi":"10.1111/1758-2229.70375","DOIUrl":"10.1111/1758-2229.70375","url":null,"abstract":"Most carbon monoxide (CO) produced in the ocean is consumed by microorganisms encoding carbon monoxide dehydrogenases (CODHs), thereby significantly reducing the flux of CO from the ocean to the atmosphere. CODHs are of two types based on the metal content of their active sites: the oxygen-sensitive, nickel-containing Ni-CODH and the oxygen-tolerant, molybdenum-copper-containing Mo-CODH. Although CODHs have been reported from specific marine environments, their combined distribution across ocean ecosystems remains unclear. Here, we analyzed the NCBI non-redundant protein database and identified 1969 Ni-CODH and 864 Mo-CODH genes from marine prokaryotes spanning diverse oceanic ecosystems. Using metagenomic analyses across three marine biomes, we showed that oxygen availability selectively constrains Ni-CODH gene abundance, but not Mo-CODHs. Thus, Ni-CODHs are restricted to oxygen-limited niches, while Mo-CODHs occur across both oxygenated and oxygen-limited marine environments. Phylogenetic analyses indicated that all previously described CODH clades are represented in the marine ecosphere, highlighting their evolutionary diversity. Genome context analyses suggest that approximately 50% of the marine Ni-CODH potentially participate in carbon fixation via the Wood-Ljungdahl pathway, whereas most marine Mo-CODH likely contribute to the supplementary energy conservation. Together, these results provide an integrated view of CODH distribution and potential function in marine ecosystems.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70375"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Resistance and Biofilm Tolerance to Antimicrobials of Marine Bacteria From Brazilian Deep-Sea Sediment. 巴西深海沉积物中海洋细菌对抗菌剂的高耐药性和生物膜耐受性。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70364

Amanda Simão Dias, Sílvia Dias de Oliveira, Renata Medina-Silva

{"title":"High Resistance and Biofilm Tolerance to Antimicrobials of Marine Bacteria From Brazilian Deep-Sea Sediment.","authors":"Amanda Simão Dias, Sílvia Dias de Oliveira, Renata Medina-Silva","doi":"10.1111/1758-2229.70364","DOIUrl":"10.1111/1758-2229.70364","url":null,"abstract":"Antimicrobial resistance studies have focused on clinical bacteria, neglecting the role of resistant isolates in natural environments. However, oceans are daily contaminated with high loads of antimicrobials and resistant bacteria from agro-industrial and urban activities. Deep-sea sediment is a challenging environment that may select microbial strains with resistance to chemicals and ability to form biofilms, becoming a potential reservoir of resistance genes. We evaluated the susceptibility to antimicrobials of six Pseudomonas sp., five Bacillus sp., two Brevibacillus sp. and two Paenibacillus sp. from deep-sea sediments of the Pelotas Basin (Brazil) by the disk diffusion and microdilution tests. Pseudomonas and Bacillales were tested against 11 and 7 antimicrobials, respectively. Biofilms of susceptible isolates were exposed to antimicrobials to determine the minimum biofilm inhibitory concentration (MBIC) and the minimum biofilm eradication concentration (MBEC). All Pseudomonas were resistant to aztreonam at very high concentrations (up to 2048 μg/mL). MBIC values were significantly higher than respective MICs, and only one third of biofilms were eradicated. These results underscore the importance of the study, as one of the first reporting antimicrobial tolerance of biofilms of cultivable bacteria from deep-sea sediments, contributing to the knowledge of bacterial resistance in these environments, concerning One Health issues.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70364"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13580871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147968989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Diversity and Functional Dynamics in the Soybean (Glycine max L.) Rhizosphere Under Different Organic Fertilisation. 大豆（Glycine max L.）细菌多样性及其功能动态不同有机肥处理下的根际土壤。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70370

Ijeoma Emelda Osuji, Akinlolu Olalekan Akanmu, Olubukola Oluranti Babalola

{"title":"Bacterial Diversity and Functional Dynamics in the Soybean (Glycine max L.) Rhizosphere Under Different Organic Fertilisation.","authors":"Ijeoma Emelda Osuji, Akinlolu Olalekan Akanmu, Olubukola Oluranti Babalola","doi":"10.1111/1758-2229.70370","DOIUrl":"10.1111/1758-2229.70370","url":null,"abstract":"Soybean (Glycine max L.) is a globally important legume for oil and protein production, yet its responses to specific organic fertilisation practices remain insufficiently understood. Organic amendments such as cattle dung and poultry manure are sustainable alternatives to inorganic fertilisers, but their effects on the soybean rhizosphere microbiome remain poorly characterised. This study investigated microbial community structure and functional diversity under poultry manure and cattle dung treatments. Rhizospheric soils were collected from treated plots, untreated controls and bulk soil and then subjected to physicochemical analysis. DNA extracted from samples was analysed using 16S rRNA gene sequencing on the Illumina NovaSeq 6000 platform, with data processed in QIIME 2 (v2019.1). Poultry manure increased available phosphorus (28%) and calcium (19%), while cattle dung enhanced potassium (22%) and magnesium (17%). Microbial community composition shifted significantly, with poultry manure promoting copiotrophic taxa such as Burkholderia and Cupriavidus and cattle dung enriching decomposers including Paenibacillus and Treponema. Alpha diversity was highest in poultry manure (Shannon index 6.2) and bulk soil (6.0) and lowest in cattle dung (5.1). Functional predictions indicated retention of core metabolic pathways, suggesting functional redundancy. Overall, organic fertilisation reshapes microbial communities while maintaining essential functions, supporting sustainable soybean cultivation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70370"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13239308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Metal Cations on Selenite Bioreduction by a Consortium of Halophilic/Halotolerant Bacteria and/or Yeast. 金属阳离子对嗜盐/耐盐细菌和/或酵母的亚硒酸盐生物还原的影响。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70320

Elham Lashani, Hamid Moghimi, Raymond J Turner, Mohammad Ali Amoozegar

{"title":"Effect of Metal Cations on Selenite Bioreduction by a Consortium of Halophilic/Halotolerant Bacteria and/or Yeast.","authors":"Elham Lashani, Hamid Moghimi, Raymond J Turner, Mohammad Ali Amoozegar","doi":"10.1111/1758-2229.70320","DOIUrl":"10.1111/1758-2229.70320","url":null,"abstract":"Selenium oxyanion pollution in the environment, often originating from anthropogenic industrial activities, causes significant risks to human health and the ecosystem. The selenite bioreduction to the less toxic form of selenium, Se (0), by microbial species and consortia offers a promising approach for bioremediation of polluted environments. This study explores the effects of potentially toxic co-contaminating metal cations, including cadmium (Cd), lead (Pb), and zinc (Zn), on the bioreduction of selenite by a consortium of halotolerant and halophilic bacteria and yeast. The effectiveness of the consortium in reducing selenite was investigated in conditions that mimic multi-metal pollution found in the real world, with varying levels of the different metals. The results showed that Pb and Cd had negative effects on the mixed consortium efficacy, while Zn showed an augmenting effect on the bacterial consortia. Also, molybdate significantly reduced selenite removal in all consortia. Kinetic analyses using the Michaelis-Menten model were applied for evaluating the rate of selenite reduction. Also, selenium biosorption and dispersion in microbial cells were studied using electron microscopy and EDS analysis. The findings provide insights into the use of microbial consortia in contaminated environments and highlight the potential for bioremediation strategies that can cope with multiple pollutants.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70320"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13580820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147968921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limnofasciculus delicatus (Coleofasciculaceae, Coleofasciculales), a Novel Mat-Forming Cyanobacterium From Shenandoah River, Virginia, USA. Limnofasciculus delicatus (Coleofasciculaceae, Coleofasciculales)，一种来自美国弗吉尼亚州Shenandoah河的新型席状蓝藻。

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70377

Rosalina Stancheva, Cecilio Valadez-Cano, Benoit Van Aken, Gordon M Selckmann, Janice Lawrence, A Bruce Cahoon

{"title":"Limnofasciculus delicatus (Coleofasciculaceae, Coleofasciculales), a Novel Mat-Forming Cyanobacterium From Shenandoah River, Virginia, USA.","authors":"Rosalina Stancheva, Cecilio Valadez-Cano, Benoit Van Aken, Gordon M Selckmann, Janice Lawrence, A Bruce Cahoon","doi":"10.1111/1758-2229.70377","DOIUrl":"10.1111/1758-2229.70377","url":null,"abstract":"Benthic cyanobacterial mats in flowing waters are complex communities typically composed of taxa from the orders Coleofasciculales and Oscillatoriales, many of which have unresolved taxonomic positions and poorly characterized toxic potential. We collected field mats of a benthic non-heterocytous filamentous cyanobacterium from the North and South Forks of the Shenandoah River in Northern Virginia (USA) that were dominated by a novel morphotype. Whole-genome and 16S rRNA gene phylogenetic analyses placed this cyanobacterium within the recently described genus Limnofasciculus (Coleofasciculaceae). Genome-based species delimitation metrics fell below accepted thresholds for bacterial species delineation relative to Limnofasciculus baicalensis, the only formally described species in the genus to date, supporting recognition of the cyanobacterium from Shenandoah River as a distinct species. Furthermore, the two Limnofasciculus species exhibited marked structural differences in the Box B helix of the 16S-23S ITS region. Comparative genomic analyses revealed a genome size similar to L. baicalensis and a conserved core gene repertoire alongside substantial divergence in biosynthetic gene cluster composition. Neither species contains biosynthetic gene clusters associated with the production of known cyanotoxins. Based on morphological, phylogenetic and genomic evidence, we describe Limnofasciculus delicatus sp. nov., supported by light microscopy and whole-genome characterization.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70377"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13283753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity, Composition and Resilience of the Root Microbiome of Tomato Plants in a Hydroponic Rockwool System. 水培岩棉系统中番茄根系微生物群的多样性、组成和恢复力

IF 3.1 4区生物学

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI: 10.1111/1758-2229.70380

Phil Thomas, Brian Sindel, Gal Winter

{"title":"Diversity, Composition and Resilience of the Root Microbiome of Tomato Plants in a Hydroponic Rockwool System.","authors":"Phil Thomas, Brian Sindel, Gal Winter","doi":"10.1111/1758-2229.70380","DOIUrl":"10.1111/1758-2229.70380","url":null,"abstract":"In hydroponic horticulture, where soil is replaced by a sterile artificial substrate, attention to microorganisms is primarily focused on the suppression of plant pathogens and the application of potentially beneficial organisms. This study examined the ecology of the root microbiome of tomato plants which were grown to maturity in a hydroponic rockwool system and then treated with a Trichoderma-based biocontrol product. The bacterial community was species-rich but was dominated by a small number of Alphaproteobacteria, Gammaproteobacteria and Bacteroidia species. The fungal community was less diverse and consisted almost exclusively of Ascomycota and Rozellomycota species. Biocontrol treatment did not have a significant effect on bacterial diversity, but the microbiome composition changed distinctly over the period of sampling in both treated and untreated plants. These results support the view that mineral substrates in a hydroponic system can support a complex and resilient root microbiome. Understanding the microorganisms that thrive in this unique environment may help identify effective biological treatments, and enable the development of rockwool-specific practices for monitoring, protecting and promoting plant health.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70380"},"PeriodicalIF":3.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13283755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0