Environmental Microbiology Reports最新文献_第8页

An in Planta Enrichment Route to Identify Bacterial Root Endophytes 植物根内生细菌的富集途径研究

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-19 DOI: 10.1111/1758-2229.70136

Denise Khouri Chalouhi, Iris Bertani, Alfonso Esposito, Silvano Piazza, Cristina Bez, Vittorio Venturi

{"title":"An in Planta Enrichment Route to Identify Bacterial Root Endophytes","authors":"Denise Khouri Chalouhi, Iris Bertani, Alfonso Esposito, Silvano Piazza, Cristina Bez, Vittorio Venturi","doi":"10.1111/1758-2229.70136","DOIUrl":"https://doi.org/10.1111/1758-2229.70136","url":null,"abstract":"Microorganisms live in close association with plants, forming ecological interaction webs and providing beneficial traits such as nutrition, growth, and tolerance to biotic and abiotic stresses. Via the rhizosphere, plants recruit bacteria which colonise internal plant tissues, creating a spatial gradient between the rhizosphere and the endosphere. This study presents a high throughput in planta endophyte enrichment scheme designed for the identification of 'super'-endophytic bacteria which can serially colonise the rice root endosphere. Oryza sativa (rice) plants were grown in bulk soil, and endophytes were then recovered from roots. The recovered endophyte mixture was used as inoculum for the first generation of rice plantlets, which were then grown under no stress or nitrogen (N) depletion. The total endophytic community was then purified and used as a second inoculum for a new set of plants; this procedure was repeated for four generations. Enrichment patterns of root bacterial endophytes were observed, such as Kosakonia in the non-stressed plants and Ferrovibrio in plants grown under nitrogen starvation. This enrichment method proved to be suitable for the identification of endophytes which can efficiently colonise the root endosphere.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Biodiversity-Function Relationships in Field Retting: Towards Key Microbial Indicators for Retting Control 加强田间冻土生物多样性-功能关系：探讨冻土控制的关键微生物指标

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-18 DOI: 10.1111/1758-2229.70102

Eliane Bou Orm, Suvajit Mukherjee, Etienne Rifa, Anne Créach, Sébastien Grec, Sandrine Bayle, Jean-Charles Benezet, Anne Bergeret, Luc Malhautier

{"title":"Enhancing Biodiversity-Function Relationships in Field Retting: Towards Key Microbial Indicators for Retting Control","authors":"Eliane Bou Orm, Suvajit Mukherjee, Etienne Rifa, Anne Créach, Sébastien Grec, Sandrine Bayle, Jean-Charles Benezet, Anne Bergeret, Luc Malhautier","doi":"10.1111/1758-2229.70102","DOIUrl":"https://doi.org/10.1111/1758-2229.70102","url":null,"abstract":"Hemp field retting is a bioprocess that facilitates fibre extraction by degrading pectin and other matrix components surrounding fibre bundles. However, traditional methods rely on empirical practices, often resulting in inconsistent fibre quality. This study investigates the biodiversity–function relationship in the hemp retting ecosystem to identify microbial and enzymatic indicators for improved process control. Over six weeks of field retting, we monitored bacterial and fungal community dynamics using high-throughput sequencing and assessed enzymatic activity profiles. Our results revealed a sequential enzymatic pattern: pectinases (e.g., polygalacturonase) dominated early stages, followed by hemicellulases (β-xylosidase, β-galactosidase), and later cellulases. These enzymatic shifts were reflected in the changes in microbial community composition, with pectinolytic bacteria (e.g., Proteobacteria) dominating the initial phases and cellulolytic fungi (e.g., Ascomycota) becoming more prevalent later. Our results identified specific microbial taxa correlated with optimal retting, suggesting their potential as bioindicators for monitoring retting. Specifically, key bacterial genera such as Pseudomonas and Sphingomonas, and fungal genera like Cladosporium, were associated with distinct enzymatic profiles. Our findings offer new insights into the microbial ecology of retting, providing both microbial and enzymatic indicators that could inform the development of monitoring strategies for process control, ultimately contributing to more consistent hemp fibre production.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum 珍贵的地中海红珊瑚早期生命阶段的微生物动态

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70127

R. Tignat-Perrier, L. Bramanti, B. Giordano, J. A. J. M. van de Water, E. Manea, D. Allemand, C. Ferrier-Pagès

{"title":"Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum","authors":"R. Tignat-Perrier, L. Bramanti, B. Giordano, J. A. J. M. van de Water, E. Manea, D. Allemand, C. Ferrier-Pagès","doi":"10.1111/1758-2229.70127","DOIUrl":"https://doi.org/10.1111/1758-2229.70127","url":null,"abstract":"Microbial colonisation in the early life stages of corals plays a critical role in their fitness, but the mechanisms by which microbial symbionts are acquired—whether from parental colonies, the environment, or both—remain poorly understood, particularly in octocorals. Although they are the second most abundant coral group on tropical reefs and contribute significantly to the structural complexity of Mediterranean coral reefs, their microbial dynamics are largely unexplored. In this study, we investigated the acquisition of the bacterial microbiota in the red coral Corallium rubrum, a precious coral. By analysing the composition of the bacterial community at different early life stages, including newly released larvae, 5- and 10-day-old larvae, 3-month-old settlers, 1-year-old recruits and 3-year-old juveniles, we are gaining new insights into the development of its microbiome. Using a direct PCR-based 16S rRNA metabarcoding approach, we performed high-resolution microbiome analyses at the level of individual larvae and settlers. Our results show that the bacterial microbiota of C. rubrum matures after the first year of life. Notably, dominant symbionts, such as Spirochaetaceae and BD72BR169 Gammaproteobacteria, were absent in larvae, settlers and recruits, suggesting that they were likely acquired horizontally from the environment. These findings improve our understanding of the microbial colonisation and development of C. rubrum and shed light on the potential role of its bacterial community in holobiont function.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromium-Tanned Leather and Microbial Consortia: Identification of Taxa With Biodegradation Potential and Chromium Tolerance 铬鞣皮革与微生物群落：具有生物降解潜力和铬耐受性的分类群鉴定

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-17 DOI: 10.1111/1758-2229.70134

Manuela Bonilla-Espadas, Irene Lifante-Martínez, Mónica Camacho, Elena Orgilés-Calpena, Francisca Arán-Aís, Marcelo Bertazzo, María-José Bonete

{"title":"Chromium-Tanned Leather and Microbial Consortia: Identification of Taxa With Biodegradation Potential and Chromium Tolerance","authors":"Manuela Bonilla-Espadas, Irene Lifante-Martínez, Mónica Camacho, Elena Orgilés-Calpena, Francisca Arán-Aís, Marcelo Bertazzo, María-José Bonete","doi":"10.1111/1758-2229.70134","DOIUrl":"https://doi.org/10.1111/1758-2229.70134","url":null,"abstract":"Chromium-tanned leather waste poses significant environmental challenges due to its resistance to degradation and heavy metal content. This study investigates the potential of naturally selected microbial consortia to initiate the degradation of chromium-tanned leather and identifies key bacterial genera capable of tolerating chromium and producing enzymes relevant to collagen breakdown. A novel multidisciplinary approach combining gravimetric assays, metagenomic sequencing, and scanning electron microscopy (SEM) was applied to characterise both microbial composition and degradation dynamics. Dominant genera such as Bacillus, Microbacterium, and Acinetobacter were associated with collagen degradation and metal tolerance, with Bacillus-rich communities showing the most pronounced mass loss (up to 3%). SEM analysis revealed the formation of robust biofilms and extensive matrix disruption, indicating enzymatic activity and structural breakdown of the leather. The formation of exopolysaccharide-rich biofilms was found to be critical for microbial adhesion and biodegradation efficacy. These findings provide initial insights into microbial mechanisms involved in the degradation of chromium-tanned leather and suggest potential applications for microbial consortia in future sustainable leather waste management strategies.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected 微氧条件下微生物生理的研究是关键但被忽视的

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-16 DOI: 10.1111/1758-2229.70108

Om Prakash, Ashvini Chauhan, Stefan J. Green

{"title":"The Study of Microbial Physiology Under Microoxic Conditions Is Critical but Neglected","authors":"Om Prakash, Ashvini Chauhan, Stefan J. Green","doi":"10.1111/1758-2229.70108","DOIUrl":"https://doi.org/10.1111/1758-2229.70108","url":null,"abstract":"During the early evolution of life on Earth, the environment was largely free of molecular oxygen, and only anaerobic life existed. With the subsequent oxidation of oceans and the atmosphere, a wide range of environmental niches, ranging from anoxic to microoxic/hypoxic and oxic, developed. Despite this broad range of natural environments, microbiology as a field has focused on the physiology, metabolism, and genetics of aerobic microorganisms, with less attention paid to anaerobes and much less attention paid to microaerophiles. The disparity in studies between aerobic and anaerobic conditions is rampant in host-associated systems, particularly in human health, and studies of microorganisms in intermediate oxygen conditions between fully aerobic and fully anoxic conditions are exceedingly rare. Studies on the physiological behaviour, metabolism, growth response, and drug susceptibility patterns of commensal and pathogenic organisms are almost totally neglected in microoxic conditions. Furthermore, microorganisms from microaerobic and microoxic ecosystems have been less robustly explored in terms of physiology, growth, and metabolism. In this work, we highlight the importance of understanding the physiological and metabolic behaviours of microorganisms under hypoxic or microoxic conditions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms 从盐环境中分离的三株盐杆菌的比较基因组分析揭示了潜在的耐盐性和杀藻机制

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-15 DOI: 10.1111/1758-2229.70121

Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh

{"title":"Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms","authors":"Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh","doi":"10.1111/1758-2229.70121","DOIUrl":"https://doi.org/10.1111/1758-2229.70121","url":null,"abstract":"Harmful algal blooms (HABs) pose a significant global threat to water ecosystems, prompting extensive research into their inhibition and control strategies. This study presents genomic and bioinformatic analyses to investigate the algicidal potential and elucidate the survival mechanisms in harsh conditions of newly identified Halobacillus species three strains (SSTM10-2T, SSBR10-3T, and SSHM10-5T) isolated from saline environments. Moreover, genomic and bioinformatic analyses were conducted to elucidate their survival mechanisms in harsh conditions. Moreover, comparative genomic analysis revealed a diverse set of orthologous genes, with a core genome primarily associated with metabolism and information processing. Pangenome analysis highlighted accessory and unique genes potentially involved in environmental adaptation and stress response. Functional annotation using KEGG pathways identified genes linked to xenobiotic compound degradation, stress tolerance, and salt adaptation. Additionally, the study elucidated potential mechanisms underlying algicidal activity, implicating Carbohydrate-Active enZYmes (CAZymes), cytochrome P450 oxidases (CYP), and quorum sensing (QS) systems. Finally, analysis of KEGG pathways related to microcystin degradation suggested the strains' capacity to mitigate HABs. Thus, this research enhances understanding of the genomic diversity, phylogeny, and functional characteristics of Halobacillus species, offering insights into their ecological roles and potential applications in biotechnology and environmental management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms 从盐环境中分离的三株盐杆菌的比较基因组分析揭示了潜在的耐盐性和杀藻机制

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-15 DOI: 10.1111/1758-2229.70121

Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh

{"title":"Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms","authors":"Saru Gurung, Chang-Muk Lee, Hang-Yeon Weon, So-Ra Han, Tae-Jin Oh","doi":"10.1111/1758-2229.70121","DOIUrl":"https://doi.org/10.1111/1758-2229.70121","url":null,"abstract":"Harmful algal blooms (HABs) pose a significant global threat to water ecosystems, prompting extensive research into their inhibition and control strategies. This study presents genomic and bioinformatic analyses to investigate the algicidal potential and elucidate the survival mechanisms in harsh conditions of newly identified Halobacillus species three strains (SSTM10-2T, SSBR10-3T, and SSHM10-5T) isolated from saline environments. Moreover, genomic and bioinformatic analyses were conducted to elucidate their survival mechanisms in harsh conditions. Moreover, comparative genomic analysis revealed a diverse set of orthologous genes, with a core genome primarily associated with metabolism and information processing. Pangenome analysis highlighted accessory and unique genes potentially involved in environmental adaptation and stress response. Functional annotation using KEGG pathways identified genes linked to xenobiotic compound degradation, stress tolerance, and salt adaptation. Additionally, the study elucidated potential mechanisms underlying algicidal activity, implicating Carbohydrate-Active enZYmes (CAZymes), cytochrome P450 oxidases (CYP), and quorum sensing (QS) systems. Finally, analysis of KEGG pathways related to microcystin degradation suggested the strains' capacity to mitigate HABs. Thus, this research enhances understanding of the genomic diversity, phylogeny, and functional characteristics of Halobacillus species, offering insights into their ecological roles and potential applications in biotechnology and environmental management.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius 硝酸盐对热葡萄共生副杆菌产氢一氧化碳氧化的影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-13 DOI: 10.1111/1758-2229.70133

Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida

{"title":"Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius","authors":"Yuka Adachi Katayama, Yoshinari Imaura, Masao Inoue, Shunsuke Okamoto, Yoshihiko Sako, Ryoma Kamikawa, Takashi Yoshida","doi":"10.1111/1758-2229.70133","DOIUrl":"https://doi.org/10.1111/1758-2229.70133","url":null,"abstract":"Parageobacillus thermoglucosidasius is a thermophilic facultative anaerobe capable of hydrogenogenic carbon monoxide (CO) oxidation utilising nickel-containing CO dehydrogenase (Ni-CODH) and energy-converting hydrogenase (ECH). Nitrates have been reported to exert promoting or inhibitory effects on the growth of CO oxidizers and acetogens, and these contradictory outcomes obscure the relationship between nitrate and CO oxidation. In this study, we analysed the effects of nitrate on hydrogenogenic CO oxidation and growth in P. thermoglucosidasius NBRC 107763T using wild-type and codh- and/or ech-disrupted strains. The results demonstrated that the addition of 50 mM nitrate suppressed hydrogenogenic CO oxidation while promoting hydrogen-oxidising nitrate reduction and rapid cell growth, resulting in a 2.3-fold higher OD600 than the control. Assays using cell lysates showed that 10 μM nitrate suppressed CO oxidation below the detection limit without affecting hydrogen production, indicating that nitrate affects the CO-oxidising function. These findings imply that CO oxidation in P. thermoglucosidasius is primarily coupled to proton reduction, and deactivated during nitrate respiration. Therefore, hydrogenogenic CO oxidation serves as an auxiliary energy-obtaining mechanism, functioning in the absence of alternative electron acceptors such as nitrate. This study enhances our understanding of CO-dependent energy generation and highlights the supplemental use of CO in P. thermoglucosidasius.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Strategies for Biocontrolling Phytopathogenic Fungi Using Trichoderma Koningiopsis LBM116 利用Koningiopsis LBM116生物防治植物病原真菌的酶促策略

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-12 DOI: 10.1111/1758-2229.70122

Natalia Soledad Amerio, Marcela Paola Barengo, Gustavo Angel Bich, Pedro Dario Zapata, Laura Lidia Villalba, María Lorena Castrillo

{"title":"Enzymatic Strategies for Biocontrolling Phytopathogenic Fungi Using Trichoderma Koningiopsis LBM116","authors":"Natalia Soledad Amerio, Marcela Paola Barengo, Gustavo Angel Bich, Pedro Dario Zapata, Laura Lidia Villalba, María Lorena Castrillo","doi":"10.1111/1758-2229.70122","DOIUrl":"https://doi.org/10.1111/1758-2229.70122","url":null,"abstract":"The growing demand for sustainable alternatives to chemical fungicides has driven the development of microbial-based biocontrol strategies. In this study, the native strain Trichoderma koningiopsis LBM116 (Misiones, Argentina) was optimised for the production of mycolytic enzymes (chitinases, β-1,3-glucanases, and proteases) using factorial and response surface experimental designs. Enzyme secretion was increased by more than 250% compared to initial conditions by selecting specific carbon and nitrogen sources and adjusting inoculum and pH parameters. The optimised enzyme formulation improved lettuce seed germination to 86.66% in the presence of the phytopathogen Fusarium sp., under controlled conditions. In seedling trials, it also reduced disease severity and improved growth parameters. These results confirm the dual effect of the enzyme formulation, acting as a biocontrol agent and plant growth promoter. This work highlights the potential of enzyme formulations derived from T. koningiopsis LBM116 as an effective, low-cost, and sustainable alternative for managing phytopathogens in agriculture.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Convergence of Nutritional Symbionts in Ticks 蜱中营养共生体的进化趋同

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-10 DOI: 10.1111/1758-2229.70120

Noor Fattar, Meriem Louni, Marie Buysse, Anna Maria Floriano, Joanne Bertaux, Anne Cantereau, Ana Rivero, Marjorie Bruley, Karen D. McCoy, Vincent Delafont, Nathalie Boulanger, Fabrice Vavre, Didier Bouchon, Olivier Duron

{"title":"Evolutionary Convergence of Nutritional Symbionts in Ticks","authors":"Noor Fattar, Meriem Louni, Marie Buysse, Anna Maria Floriano, Joanne Bertaux, Anne Cantereau, Ana Rivero, Marjorie Bruley, Karen D. McCoy, Vincent Delafont, Nathalie Boulanger, Fabrice Vavre, Didier Bouchon, Olivier Duron","doi":"10.1111/1758-2229.70120","DOIUrl":"https://doi.org/10.1111/1758-2229.70120","url":null,"abstract":"Symbiosis with bacteria is essential for the survival of animals with an obligate blood-feeding lifestyle. In ticks, two distinct bacterial lineages, Coxiella-like and Francisella-like endosymbionts, have independently evolved into nutritional symbionts, converging on a key biochemical function for the tick's survival and growth: the production of three B vitamins. In this study, we carried out comparative analyses across multiple tick species and characterised remarkable similarities in their tissue localisation, particularly in organs important for nutrient metabolism and maternal transmission to progeny. In these organs, both symbionts colonise similar intracellular niches, residing within membrane-bound, replicative vacuoles that occupy a substantial part of the cytoplasm of tick cells. Despite extensive genomic reduction, both symbionts have retained pathways for the biosynthesis of B vitamins and, in some cases, chorismate, a precursor used for the production of serotonin by ticks. However, differences exist: while Coxiella-like endosymbionts lack the ability to synthesise heme, Francisella-like endosymbionts possess a complete heme biosynthesis pathway and may potentially provide ticks with this essential cofactor. Overall, these phenotypic and genomic characteristics reveal a broad convergence among symbiotic interactions across major tick families, highlighting the essential role of symbiosis in tick nutrition, feeding behaviour, blood intake and subsequently in pathogen transmission.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0