Environmental Microbiology Reports最新文献

{"title":"Environmental Microbiome of Tyrophagus Putrescentiae Culture and Its Changes in Manipulative Experiments","authors":"Jan Hubert,&nbsp;Bruno Sopko,&nbsp;Eliza Głowska-Patyniak","doi":"10.1111/1758-2229.70142","DOIUrl":"10.1111/1758-2229.70142","url":null,"abstract":"<p>Storage mites consume stored products in interaction with environmental microorganisms, resulting in the destruction of infested food and providing specific odours. Here we simulated the effect of mite grazing on oat flakes. Spent growth medium (SPGM) was obtained from seven mite cultures and mixed with oat flakes as the source of faeces and microbes. SPGM-treated diets were offered to 4 mite cultures. The microbiomes were analysed using sequencing of V4_16S_DNA. Mite growth tests, food preferences, and microbiome changes were observed in correlation with SPGM type and mite cultures. The microbiome consisted of 41 OTUs belonging to mite-associated bacteria and faeces bacteria. The composition of the microbiome depends more on the source of SPGM than on mite culture. The SPGM diet accelerated mite population growth and influenced mite food choice, although the effect was dependent on both types of SPGM and mite culture. <i>Kocuria</i>, <i>Brevibacterium</i>, <i>Virgibacillus</i>, and <i>Staphylococcus</i> profiles in SPGM added into diets showed positive correlations to mite population growth. The <i>Kocuria</i> profile in the bodies of mites was positively correlated with mite population growth. The results showed that mites are influenced by SPGM-treated diets, and mite feeding influences the environmental microbiome. The most beneficial was the mite interaction with <i>Kocuria</i>.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water","authors":"Jirachaya Toyting-Hiraishi,&nbsp;Toyotaka Sato,&nbsp;Neunghatai Supha,&nbsp;Yuwanda Thongpanich,&nbsp;Motohiro Horiuchi,&nbsp;Jeewan Thapa,&nbsp;Chie Nakajima,&nbsp;Yasuhiko Suzuki,&nbsp;Fuangfa Utrarachkij","doi":"10.1111/1758-2229.70160","DOIUrl":"https://doi.org/10.1111/1758-2229.70160","url":null,"abstract":"<p><i>Salmonella</i> is one of the important pathogens causing acute gastroenteritis, and antimicrobial-resistant <i>Salmonella</i> raises a critical public health concern. Canals in Bangkok, Thailand, play a vital role as sources of agricultural and daily water usage. By employing whole genome sequencing to analyse 351 <i>Salmonella</i> genomes isolated between 2016 and 2020, we expanded the understanding of the characteristics and antimicrobial resistance properties of <i>Salmonella enterica</i> found in Bangkok canals, an underrepresented biome in research. <i>Salmonella</i> Agona was the dominant serotype, while S. Typhimurium and its monophasic variant were periodically found. Seven new sequence types (STs) were identified, including STs 11,346, 11,347, 11,348, 11,349, 11,350, 11,351, and 11,352. Seven chromosomal-mediated gene mutations and 50 antimicrobial resistance genes were detected. The three most common resistance genes were <i>tet</i>(A), <i>bla</i>_TEM-1B, and <i>qnrS1</i>. The <i>tet</i>(X4) was first identified in the <i>Salmonella</i> population in Thailand, and <i>mcr-3.1</i> was also detected. In total, 39.0% of the strains were potentially multidrug-resistant. The strains carried double amino acid substitutions in GyrA and ParC, and a strain with GyrA substitutions and <i>qnrS1</i> exhibited the strongest resistance to nalidixic acid and ciprofloxacin. Most of the ceftazidime, ceftriaxone, and cefotaxime-resistant strains (66.7%) harboured <i>bla</i>_CTX-M-55. Col(pHAD28) was the predominant plasmid replicon type. Phylogenetic analysis of <i>Salmonella</i> STs 34 and 213 from canal water and the strains from databases showed the possibility of circulation of STs 34 and 213 between canal water and humans in Thailand and worldwide. These findings shed light on the circulation of antimicrobial-resistant pathogens in the environmental water and advocate for incorporating environmental sampling into comprehensive AMR surveillance programmes within a One Health framework.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water","authors":"Jirachaya Toyting-Hiraishi,&nbsp;Toyotaka Sato,&nbsp;Neunghatai Supha,&nbsp;Yuwanda Thongpanich,&nbsp;Motohiro Horiuchi,&nbsp;Jeewan Thapa,&nbsp;Chie Nakajima,&nbsp;Yasuhiko Suzuki,&nbsp;Fuangfa Utrarachkij","doi":"10.1111/1758-2229.70160","DOIUrl":"10.1111/1758-2229.70160","url":null,"abstract":"<p><i>Salmonella</i> is one of the important pathogens causing acute gastroenteritis, and antimicrobial-resistant <i>Salmonella</i> raises a critical public health concern. Canals in Bangkok, Thailand, play a vital role as sources of agricultural and daily water usage. By employing whole genome sequencing to analyse 351 <i>Salmonella</i> genomes isolated between 2016 and 2020, we expanded the understanding of the characteristics and antimicrobial resistance properties of <i>Salmonella enterica</i> found in Bangkok canals, an underrepresented biome in research. <i>Salmonella</i> Agona was the dominant serotype, while S. Typhimurium and its monophasic variant were periodically found. Seven new sequence types (STs) were identified, including STs 11,346, 11,347, 11,348, 11,349, 11,350, 11,351, and 11,352. Seven chromosomal-mediated gene mutations and 50 antimicrobial resistance genes were detected. The three most common resistance genes were <i>tet</i>(A), <i>bla</i>_TEM-1B, and <i>qnrS1</i>. The <i>tet</i>(X4) was first identified in the <i>Salmonella</i> population in Thailand, and <i>mcr-3.1</i> was also detected. In total, 39.0% of the strains were potentially multidrug-resistant. The strains carried double amino acid substitutions in GyrA and ParC, and a strain with GyrA substitutions and <i>qnrS1</i> exhibited the strongest resistance to nalidixic acid and ciprofloxacin. Most of the ceftazidime, ceftriaxone, and cefotaxime-resistant strains (66.7%) harboured <i>bla</i>_CTX-M-55. Col(pHAD28) was the predominant plasmid replicon type. Phylogenetic analysis of <i>Salmonella</i> STs 34 and 213 from canal water and the strains from databases showed the possibility of circulation of STs 34 and 213 between canal water and humans in Thailand and worldwide. These findings shed light on the circulation of antimicrobial-resistant pathogens in the environmental water and advocate for incorporating environmental sampling into comprehensive AMR surveillance programmes within a One Health framework.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking and Treating Fungal Contamination in Indoor-Growing Barley Sprouts","authors":"Dedong Kong,&nbsp;Mengdi Dai,&nbsp;Ziran Ye,&nbsp;Yu Luo,&nbsp;Xuting Chen,&nbsp;Xiangfeng Tan","doi":"10.1111/1758-2229.70161","DOIUrl":"10.1111/1758-2229.70161","url":null,"abstract":"<p>The industrialised production of barley sprouts is a nutritional replenish for livestock, whereas it is being threatened by fungal contamination derived from the closed and humid environment. This study investigates the fungal communities in barley seeds and sprouts and explores the utilisation of ozone water as a mould control method. In barley seeds of 10 cultivars, <i>Alternaria alternata</i>, <i>Phoma epicoccina</i> and <i>Fusarium cerealis</i> were the most abundant fungal species and varied between barley cultivars. A significant transformation in fungal communities after seed germination was observed, featured by the shifted community structure and a significant decline of alpha diversity in the eight-day sprouts. <i>Arthroderma vanbreuseghemii</i>, <i>Fusarium cerealis</i> and <i>Candida quercitrusa</i> were identified to proliferate in eight-day barley sprouts. Ozone water treatment was effective in suppressing fungal contamination including <i>A. vanbreuseghemii</i> and several <i>Fusarium</i> spp. Among the volatile organic compounds, the abundance of 3-Octanone isomers was significantly reduced by ozone water treatment, suggesting its potential role as a volatile marker for monitoring mould outbreak. Our research emphasises cultivar-specific fungal profiles in the production of barley sprouts and proposes ozone water as an effective control measure to ensure the safety of barley sprouts.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking and Treating Fungal Contamination in Indoor-Growing Barley Sprouts","authors":"Dedong Kong,&nbsp;Mengdi Dai,&nbsp;Ziran Ye,&nbsp;Yu Luo,&nbsp;Xuting Chen,&nbsp;Xiangfeng Tan","doi":"10.1111/1758-2229.70161","DOIUrl":"10.1111/1758-2229.70161","url":null,"abstract":"<p>The industrialised production of barley sprouts is a nutritional replenish for livestock, whereas it is being threatened by fungal contamination derived from the closed and humid environment. This study investigates the fungal communities in barley seeds and sprouts and explores the utilisation of ozone water as a mould control method. In barley seeds of 10 cultivars, <i>Alternaria alternata</i>, <i>Phoma epicoccina</i> and <i>Fusarium cerealis</i> were the most abundant fungal species and varied between barley cultivars. A significant transformation in fungal communities after seed germination was observed, featured by the shifted community structure and a significant decline of alpha diversity in the eight-day sprouts. <i>Arthroderma vanbreuseghemii</i>, <i>Fusarium cerealis</i> and <i>Candida quercitrusa</i> were identified to proliferate in eight-day barley sprouts. Ozone water treatment was effective in suppressing fungal contamination including <i>A. vanbreuseghemii</i> and several <i>Fusarium</i> spp. Among the volatile organic compounds, the abundance of 3-Octanone isomers was significantly reduced by ozone water treatment, suggesting its potential role as a volatile marker for monitoring mould outbreak. Our research emphasises cultivar-specific fungal profiles in the production of barley sprouts and proposes ozone water as an effective control measure to ensure the safety of barley sprouts.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-Engaged Course-Based Undergraduate Research of Multidrug Resistance in Escherichia coli in Water Near Dairy and Hog Farms in Michigan","authors":"Renee Chowdhry,&nbsp;Soeun Jun,&nbsp;Yuwei Kong,&nbsp;Adrian Casillas Saenz,&nbsp;Jonathan Chung,&nbsp;Michelle Chang,&nbsp;Katie Osborn,&nbsp;Yuhui Zhang,&nbsp;Will Bodeau,&nbsp;Nicole Curristan,&nbsp;Brynn Sofro,&nbsp;Soham Ray,&nbsp;Karina Jimenez,&nbsp;Lynn Henning,&nbsp;Cole Dickerson,&nbsp;Salman Jaberi,&nbsp;Clare Delucchi,&nbsp;Jose Reyes Miranda,&nbsp;Adriane Jones,&nbsp;Carol Bascom-Slack,&nbsp;Jennifer A. Jay","doi":"10.1111/1758-2229.70151","DOIUrl":"https://doi.org/10.1111/1758-2229.70151","url":null,"abstract":"<p>Antimicrobial resistance (AMR) was assessed in Michigan surface waters impacted by dairies, swine farms and human wastewater, as well as in an unimpacted (UI) comparison site. <i>Escherichia coli</i> (EC) was quantified in the presence and absence of cefotaxime, as extended-spectrum beta-lactamase-producing EC (ESBL-EC) has been deemed a proxy for AMR. Purified isolates of EC selected without antibiotics were characterised by disk diffusion; antibiotics tested included ampicillin, tetracycline, cefotaxime, cefoxitin, streptomycin, nalidixic acid, kanamycin, ciprofloxacin and erythromycin. Ampicillin and tetracycline resistance ranged up to 67% and 62% of the EC isolates, respectively, at livestock-impacted sites, but were low at UI. Multidrug resistance (MDR) was not observed at all at UI but was observed in up to 76% and 67% of isolates from dairy and swine/dairy, respectively. AMP-TE-E was the most common resistance pattern observed, with all isolates originating from one of the dairy sites. Notably, resistance to cefotaxime did not correlate with MDR, indicating that preselection for ESBL-EC before further AMR testing will not successfully characterise AMR or MDR from culturable EC. Interestingly, the percent of isolates resistant to AMP correlated quite well with MDR. This work highlights the importance of MDR characterisation at livestock-impacted surface water sites.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Halophilic and Halotolerant Bacterial Strains, Screening for Bioactive Compounds and Characterisation of Metabolites Produced by Pseudoalteromonas sp. ASV78","authors":"Maia Azpiazu-Muniozguren,&nbsp;Elena Valgañón-Pérez,&nbsp;Minerva García-Martínez,&nbsp;Alba Rodriguez-Paniagua,&nbsp;H. Poppy Clark,&nbsp;Carlos Justicia,&nbsp;Jesús Martín,&nbsp;Mercedes de la Cruz Moreno,&nbsp;Fernando Reyes,&nbsp;Lorena Laorden,&nbsp;Irati Martinez-Malaxetxebarria,&nbsp;Ilargi Martinez-Ballesteros","doi":"10.1111/1758-2229.70159","DOIUrl":"10.1111/1758-2229.70159","url":null,"abstract":"<p>Halophilic and halotolerant microorganisms are an important source of natural products. In this study, a collection of 150 bacterial isolates from a continental saltern (Añana Salt Valley, Spain) was obtained, identified and screened for the production of antimicrobial and/or surface-active compounds. The identification of the isolates showed the predominance of the <i>Pseudomonadota</i> phylum with 106 isolates (70.7%) classified under this taxon. Subsequent bioassays identified 20 antimicrobial producers and 14 isolates with the ability to both reduce surface tension and emulsify. One of them, namely, ASV78 was characterised in detail. <i>Pseudoalteromonas</i> sp. ASV78 showed simultaneous production of pentabromopseudilin and bromophene, with antibacterial activity, as well as glycolipids with the ability to reduce surface tension and glycoproteins with effective emulsifying properties. The low concentration of pentabromopseudilin (MIC values ranging from 0.02 to 0.04 μg/mL) at which it is effective against <i>S. aureus</i> ATCC 29213 was noteworthy, as was the compound's activity against other clinically relevant Gram-negative and Gram-positive bacteria. The presence of biosynthetic gene clusters (BGCs) in the genome of strain ASV78 that are not included among the known BGCs suggests the possibility that they encode unknown molecules. The applicability of the compounds synthesised by <i>Pseudoalteromonas</i> sp. ASV78 and the potential of the isolate collection require further investigation.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-Engaged Course-Based Undergraduate Research of Multidrug Resistance in Escherichia coli in Water Near Dairy and Hog Farms in Michigan","authors":"Renee Chowdhry,&nbsp;Soeun Jun,&nbsp;Yuwei Kong,&nbsp;Adrian Casillas Saenz,&nbsp;Jonathan Chung,&nbsp;Michelle Chang,&nbsp;Katie Osborn,&nbsp;Yuhui Zhang,&nbsp;Will Bodeau,&nbsp;Nicole Curristan,&nbsp;Brynn Sofro,&nbsp;Soham Ray,&nbsp;Karina Jimenez,&nbsp;Lynn Henning,&nbsp;Cole Dickerson,&nbsp;Salman Jaberi,&nbsp;Clare Delucchi,&nbsp;Jose Reyes Miranda,&nbsp;Adriane Jones,&nbsp;Carol Bascom-Slack,&nbsp;Jennifer A. Jay","doi":"10.1111/1758-2229.70151","DOIUrl":"10.1111/1758-2229.70151","url":null,"abstract":"<p>Antimicrobial resistance (AMR) was assessed in Michigan surface waters impacted by dairies, swine farms and human wastewater, as well as in an unimpacted (UI) comparison site. <i>Escherichia coli</i> (EC) was quantified in the presence and absence of cefotaxime, as extended-spectrum beta-lactamase-producing EC (ESBL-EC) has been deemed a proxy for AMR. Purified isolates of EC selected without antibiotics were characterised by disk diffusion; antibiotics tested included ampicillin, tetracycline, cefotaxime, cefoxitin, streptomycin, nalidixic acid, kanamycin, ciprofloxacin and erythromycin. Ampicillin and tetracycline resistance ranged up to 67% and 62% of the EC isolates, respectively, at livestock-impacted sites, but were low at UI. Multidrug resistance (MDR) was not observed at all at UI but was observed in up to 76% and 67% of isolates from dairy and swine/dairy, respectively. AMP-TE-E was the most common resistance pattern observed, with all isolates originating from one of the dairy sites. Notably, resistance to cefotaxime did not correlate with MDR, indicating that preselection for ESBL-EC before further AMR testing will not successfully characterise AMR or MDR from culturable EC. Interestingly, the percent of isolates resistant to AMP correlated quite well with MDR. This work highlights the importance of MDR characterisation at livestock-impacted surface water sites.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Halophilic and Halotolerant Bacterial Strains, Screening for Bioactive Compounds and Characterisation of Metabolites Produced by Pseudoalteromonas sp. ASV78","authors":"Maia Azpiazu-Muniozguren,&nbsp;Elena Valgañón-Pérez,&nbsp;Minerva García-Martínez,&nbsp;Alba Rodriguez-Paniagua,&nbsp;H. Poppy Clark,&nbsp;Carlos Justicia,&nbsp;Jesús Martín,&nbsp;Mercedes de la Cruz Moreno,&nbsp;Fernando Reyes,&nbsp;Lorena Laorden,&nbsp;Irati Martinez-Malaxetxebarria,&nbsp;Ilargi Martinez-Ballesteros","doi":"10.1111/1758-2229.70159","DOIUrl":"10.1111/1758-2229.70159","url":null,"abstract":"<p>Halophilic and halotolerant microorganisms are an important source of natural products. In this study, a collection of 150 bacterial isolates from a continental saltern (Añana Salt Valley, Spain) was obtained, identified and screened for the production of antimicrobial and/or surface-active compounds. The identification of the isolates showed the predominance of the <i>Pseudomonadota</i> phylum with 106 isolates (70.7%) classified under this taxon. Subsequent bioassays identified 20 antimicrobial producers and 14 isolates with the ability to both reduce surface tension and emulsify. One of them, namely, ASV78 was characterised in detail. <i>Pseudoalteromonas</i> sp. ASV78 showed simultaneous production of pentabromopseudilin and bromophene, with antibacterial activity, as well as glycolipids with the ability to reduce surface tension and glycoproteins with effective emulsifying properties. The low concentration of pentabromopseudilin (MIC values ranging from 0.02 to 0.04 μg/mL) at which it is effective against <i>S. aureus</i> ATCC 29213 was noteworthy, as was the compound's activity against other clinically relevant Gram-negative and Gram-positive bacteria. The presence of biosynthetic gene clusters (BGCs) in the genome of strain ASV78 that are not included among the known BGCs suggests the possibility that they encode unknown molecules. The applicability of the compounds synthesised by <i>Pseudoalteromonas</i> sp. ASV78 and the potential of the isolate collection require further investigation.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community Structure and Function of Aerobic Methanotrophs in Urban River Sediments: A Case Study of the Jialing River","authors":"Yongliang Mo,&nbsp;Jiuling Huang,&nbsp;Maoli Hu,&nbsp;Lu Lu,&nbsp;Muhammad Shaaban","doi":"10.1111/1758-2229.70167","DOIUrl":"10.1111/1758-2229.70167","url":null,"abstract":"<p>Rivers are hotspots of global methane emission and oxidation, yet research on aerobic methanotrophy in urban river sediments remains limited. Here, we collected sediments of the Jialing River and its tributary (Xixi River) from the urban area of Nanchong City (Sichuan Province, China), and used microcosm incubation and high-throughput sequencing methods to investigate the aerobic methane oxidation rate and the community structure of methanotrophs of these urban river sediments. Our results indicated that these urban river sediments exhibited substantial methane oxidation potential, with the maximum rate observed at the downstream site; high-throughput sequencing revealed that type I methanotrophs (<i>Methylobacter</i>, <i>Methylomicrobium</i> and <i>Crenothrix</i>) were the key microbial groups responsible for aerobic methane oxidation. The river physico-chemical properties such as dissolved organic carbon (DOC), total carbon (TC) and C/N ratio correlated significantly with aerobic methane oxidation rates. These findings suggest that urban rivers possess significant methane oxidation potential, which is notably affected by carbon and nitrogen contents. Future research should focus on the metabolic mechanisms of urbanisation on fluvial methanotrophy.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}