Environmental Microbiology Reports最新文献_第8页

Comparative metabolic profiling of the mycelium and fermentation broth of Penicillium restrictum from Peucedanum praeruptorum rhizosphere 对 Peucedanum praeruptorum 根瘤菌中限制青霉的菌丝体和发酵液进行比较代谢分析。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-06-06 DOI: 10.1111/1758-2229.13286

Yuanyuan Wang, Ranran Liao, Haoyu Pan, Xuejun Wang, Xiaoting Wan, Bangxing Han, Cheng Song

{"title":"Comparative metabolic profiling of the mycelium and fermentation broth of Penicillium restrictum from Peucedanum praeruptorum rhizosphere","authors":"Yuanyuan Wang, Ranran Liao, Haoyu Pan, Xuejun Wang, Xiaoting Wan, Bangxing Han, Cheng Song","doi":"10.1111/1758-2229.13286","DOIUrl":"10.1111/1758-2229.13286","url":null,"abstract":"Microorganisms in the rhizosphere, particularly arbuscular mycorrhiza, have a broad symbiotic relationship with their host plants. One of the major fungi isolated from the rhizosphere of Peucedanum praeruptorum is Penicillium restrictum. The relationship between the metabolites of P. restrictum and the root exudates of P. praeruptorum is being investigated. The accumulation of metabolites in the mycelium and fermentation broth of P. restrictum was analysed over different fermentation periods. Non-targeted metabolomics was used to compare the differences in intracellular and extracellular metabolites over six periods. There were significant differences in the content and types of mycelial metabolites during the incubation. Marmesin, an important intermediate in the biosynthesis of coumarins, was found in the highest amount on the fourth day of incubation. The differential metabolites were screened to obtain 799 intracellular and 468 extracellular differential metabolites. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the highly enriched extracellular metabolic pathways were alanine, aspartate and glutamate metabolism, glyoxylate and dicarboxylate metabolism, and terpenoid backbone biosynthesis. In addition, the enrichment analysis associated with intracellular and extracellular ATP-binding cassette transporter proteins revealed that some ATP-binding cassette transporters may be involved in the transportation of certain amino acids and carbohydrates. Our results provide some theoretical basis for the regulatory mechanisms between the rhizosphere and the host plant and pave the way for the heterologous production of furanocoumarin.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13286","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracoccus kondratievae produces poly(3-hydroxybutyrate) under elevated temperature conditions Paracoccus kondratievae 在高温条件下产生聚（3-羟基丁酸）物质

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-06-05 DOI: 10.1111/1758-2229.13260

Radwa Moanis, Hannelore Geeraert, Niko Van den Brande, Ulrich Hennecke, Eveline Peeters

{"title":"Paracoccus kondratievae produces poly(3-hydroxybutyrate) under elevated temperature conditions","authors":"Radwa Moanis, Hannelore Geeraert, Niko Van den Brande, Ulrich Hennecke, Eveline Peeters","doi":"10.1111/1758-2229.13260","DOIUrl":"https://doi.org/10.1111/1758-2229.13260","url":null,"abstract":"As part of ongoing efforts to discover novel polyhydroxyalkanoate-producing bacterial species, we embarked on characterizing the thermotolerant species, Paracoccus kondratievae, for biopolymer synthesis. Using traditional chemical and thermal characterization techniques, we found that P. kondratievae accumulates poly(3-hydroxybutyrate) (PHB), reaching up to 46.8% of the cell's dry weight after a 24-h incubation at 42°C. Although P. kondratievae is phylogenetically related to the prototypical polyhydroxyalkanoate producer, Paracoccus denitrificans, we observed significant differences in the PHB production dynamics between these two Paracoccus species. Notably, P. kondratievae can grow and produce PHB at elevated temperatures ranging from 42 to 47°C. Furthermore, P. kondratievae reaches its peak PHB content during the early stationary growth phase, specifically after 24 h of growth in a flask culture. This is then followed by a decline in the later stages of the stationary growth phase. The depolymerization observed in this growth phase is facilitated by the abundant presence of the PhaZ depolymerase enzyme associated with PHB granules. We observed the highest PHB levels when the cells were cultivated in a medium with glycerol as the sole carbon source and a carbon-to-nitrogen ratio of 10. Finally, we found that PHB production is induced as an osmotic stress response, similar to other polyhydroxyalkanoate-producing species.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141251464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracoccus kondratievae produces poly(3-hydroxybutyrate) under elevated temperature conditions Paracoccus kondratievae 在高温条件下产生聚（3-羟基丁酸）物质

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-06-05 DOI: 10.1111/1758-2229.13260

Radwa Moanis, Hannelore Geeraert, Niko Van den Brande, Ulrich Hennecke, Eveline Peeters

{"title":"Paracoccus kondratievae produces poly(3-hydroxybutyrate) under elevated temperature conditions","authors":"Radwa Moanis, Hannelore Geeraert, Niko Van den Brande, Ulrich Hennecke, Eveline Peeters","doi":"10.1111/1758-2229.13260","DOIUrl":"https://doi.org/10.1111/1758-2229.13260","url":null,"abstract":"As part of ongoing efforts to discover novel polyhydroxyalkanoate-producing bacterial species, we embarked on characterizing the thermotolerant species, Paracoccus kondratievae, for biopolymer synthesis. Using traditional chemical and thermal characterization techniques, we found that P. kondratievae accumulates poly(3-hydroxybutyrate) (PHB), reaching up to 46.8% of the cell's dry weight after a 24-h incubation at 42°C. Although P. kondratievae is phylogenetically related to the prototypical polyhydroxyalkanoate producer, Paracoccus denitrificans, we observed significant differences in the PHB production dynamics between these two Paracoccus species. Notably, P. kondratievae can grow and produce PHB at elevated temperatures ranging from 42 to 47°C. Furthermore, P. kondratievae reaches its peak PHB content during the early stationary growth phase, specifically after 24 h of growth in a flask culture. This is then followed by a decline in the later stages of the stationary growth phase. The depolymerization observed in this growth phase is facilitated by the abundant presence of the PhaZ depolymerase enzyme associated with PHB granules. We observed the highest PHB levels when the cells were cultivated in a medium with glycerol as the sole carbon source and a carbon-to-nitrogen ratio of 10. Finally, we found that PHB production is induced as an osmotic stress response, similar to other polyhydroxyalkanoate-producing species.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141251463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential requirement for RecFOR pathway components in Thermus thermophilus 嗜热菌对 RecFOR 途径成分的不同需求。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-06-01 DOI: 10.1111/1758-2229.13269

Cristina L. Gómez-Campo, Ali Abdelmoteleb, Verónica Pulido, Marc Gost, Dione L. Sánchez-Hevia, José Berenguer, Mario Mencía

{"title":"Differential requirement for RecFOR pathway components in Thermus thermophilus","authors":"Cristina L. Gómez-Campo, Ali Abdelmoteleb, Verónica Pulido, Marc Gost, Dione L. Sánchez-Hevia, José Berenguer, Mario Mencía","doi":"10.1111/1758-2229.13269","DOIUrl":"10.1111/1758-2229.13269","url":null,"abstract":"Recombinational repair is an important mechanism that allows DNA replication to overcome damaged templates, so the DNA is duplicated timely and correctly. The RecFOR pathway is one of the common ways to load RecA, while the RuvABC complex operates in the resolution of DNA intermediates. We have generated deletions of recO, recR and ruvB genes in Thermus thermophilus, while a recF null mutant could not be obtained. The recO deletion was in all cases accompanied by spontaneous loss of function mutations in addA or addB genes, which encode a helicase-exonuclease also key for recombination. The mutants were moderately affected in viability and chromosome segregation. When we generated these mutations in a Δppol/addAB strain, we observed that the transformation efficiency was maintained at the typical level of Δppol/addAB, which is 100-fold higher than that of the wild type. Most mutants showed increased filamentation phenotypes, especially ruvB, which also had DNA repair defects. These results suggest that in T. thermophilus (i) the components of the RecFOR pathway have differential roles, (ii) there is an epistatic relationship of the AddAB complex over the RecFOR pathway and (iii) that neither of the two pathways or their combination is strictly required for viability although they are necessary for normal DNA repair and chromosome segregation.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13269","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141185588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tolerance to environmental pollution in the freshwater crustacean Asellus aquaticus: A role for the microbiome 淡水甲壳动物 Asellus aquaticus 对环境污染的耐受性：微生物组的作用

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-23 DOI: 10.1111/1758-2229.13252

Terézia Horváthová, Elvira Lafuente, Jo-Anne Bartels, Jesper Wallisch, Christoph Vorburger

{"title":"Tolerance to environmental pollution in the freshwater crustacean Asellus aquaticus: A role for the microbiome","authors":"Terézia Horváthová, Elvira Lafuente, Jo-Anne Bartels, Jesper Wallisch, Christoph Vorburger","doi":"10.1111/1758-2229.13252","DOIUrl":"10.1111/1758-2229.13252","url":null,"abstract":"Freshwater habitats are frequently contaminated by diverse chemicals of anthropogenic origin, collectively referred to as micropollutants, that can have detrimental effects on aquatic life. The animals' tolerance to micropollutants may be mediated by their microbiome. If polluted aquatic environments select for contaminant-degrading microbes, the acquisition of such microbes by the host may increase its tolerance to pollution. Here we tested for the potential effects of the host microbiome on the growth and survival of juvenile Asellus aquaticus, a widespread freshwater crustacean. Using faecal microbiome transplants, we provided newly hatched juveniles with the microbiome isolated from donor adults reared in either clean or micropollutant-contaminated water and, after transplantation, recipient juveniles were reared in water with and without micropollutants. The experiment revealed a significant negative effect of the micropollutants on the survival of juvenile isopods regardless of the received faecal microbiome. The micropollutants had altered the composition of the bacterial component of the donors' microbiome, which in turn influenced the microbiome of juvenile recipients. Hence, we show that relatively high environmental concentrations of micropollutants reduce survival and alter the microbiome composition of juvenile A. aquaticus, but we have no evidence that tolerance to micropollutants is modulated by their microbiome.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leafcutter ants enhance microbial drought resilience in tropical forest soil 切叶蚁增强了热带森林土壤中微生物的抗旱能力。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-23 DOI: 10.1111/1758-2229.13251

Hannah B. Shulman, Emma L. Aronson, Diego Dierick, Andrian A. Pinto-Tomás, Jon K. Botthoff, Allan Artavia-León, Michael F. Allen

{"title":"Leafcutter ants enhance microbial drought resilience in tropical forest soil","authors":"Hannah B. Shulman, Emma L. Aronson, Diego Dierick, Andrian A. Pinto-Tomás, Jon K. Botthoff, Allan Artavia-León, Michael F. Allen","doi":"10.1111/1758-2229.13251","DOIUrl":"10.1111/1758-2229.13251","url":null,"abstract":"We conducted a research campaign in a neotropical rainforest in Costa Rica throughout the drought phase of an El-Nino Southern Oscillation event to determine microbial community dynamics and soil C fluxes. Our study included nests of the leafcutter ant Atta cephalotes, as soil disturbances made by these ecosystem engineers may influence microbial drought response. Drought decreased the diversity of microbes and the abundance of core microbiome taxa, including Verrucomicrobial bacteria and Sordariomycete fungi. Despite initial responses of decreasing diversity and altered composition, 6 months post-drought the microbiomes were similar to pre-drought conditions, demonstrating the resilience of soil microbial communities to drought events. A. cephalotes nests altered fungal composition in the surrounding soil, and reduced both fungal mortality and growth of Acidobacteria post-drought. Drought increased CH4 consumption in soils due to lower soil moisture, and A. cephalotes nests decrease the variability of CH4 emissions in some soil types. CH4 emissions were tracked by the abundance of methanotrophic bacteria and fungal composition. These results characterize the microbiome of tropical soils across both time and space during drought and provide evidence for the importance of leafcutter ant nests in shaping soil microbiomes and enhancing microbial resilience during climatic perturbations.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterotrophic bacteria trigger transcriptome remodelling in the photosynthetic picoeukaryote Micromonas commoda 异养菌引发光合微核生物小龙虾转录组重塑

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-22 DOI: 10.1111/1758-2229.13285

Maria Hamilton, Frank Xavier Ferrer-González, Mary Ann Moran

{"title":"Heterotrophic bacteria trigger transcriptome remodelling in the photosynthetic picoeukaryote Micromonas commoda","authors":"Maria Hamilton, Frank Xavier Ferrer-González, Mary Ann Moran","doi":"10.1111/1758-2229.13285","DOIUrl":"10.1111/1758-2229.13285","url":null,"abstract":"Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. Details of these associations are not well understood, especially in the case of direct influences of bacteria on phytoplankton physiology. Here we catalogue how the presence of three marine bacteria (Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14 and Polaribacter dokdonensis MED152) individually and uniquely impact gene expression of the picoeukaryotic alga Micromonas commoda RCC 299. We find a dramatic transcriptomic remodelling by M. commoda after 8 h in co-culture, followed by an increase in cell numbers by 56 h compared with the axenic cultures. Some aspects of the algal transcriptomic response are conserved across all three bacterial co-cultures, including an unexpected reduction in relative expression of photosynthesis and carbon fixation pathways. Expression differences restricted to a single bacterium are also observed, with the Flavobacteriia P. dokdonensis uniquely eliciting changes in relative expression of algal genes involved in biotin biosynthesis and the acquisition and assimilation of nitrogen. This study reveals that M. commoda has rapid and extensive responses to heterotrophic bacteria in ways that are generalizable, as well as in a taxon specific manner, with implications for the diversity of phytoplankton-bacteria interactions ongoing in the surface ocean.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria on the foundational kelp in kelp forest ecosystems: Insights from culturing, whole genome sequencing and metabolic assays 海藻森林生态系统中基础海藻上的细菌：从培养、全基因组测序和代谢测定中获得的启示。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-22 DOI: 10.1111/1758-2229.13270

Isaac T. Younker, Nichos Molnar, Kaylie Scorza, Roo Weed, Samuel H. Light, Catherine A. Pfister

{"title":"Bacteria on the foundational kelp in kelp forest ecosystems: Insights from culturing, whole genome sequencing and metabolic assays","authors":"Isaac T. Younker, Nichos Molnar, Kaylie Scorza, Roo Weed, Samuel H. Light, Catherine A. Pfister","doi":"10.1111/1758-2229.13270","DOIUrl":"10.1111/1758-2229.13270","url":null,"abstract":"In coastal marine ecosystems, kelp forests serve as a vital habitat for numerous species and significantly influence local nutrient cycles. Bull kelp, or Nereocystis luetkeana, is a foundational species in the iconic kelp forests of the northeast Pacific Ocean and harbours a complex microbial community with potential implications for kelp health. Here, we report the isolation and functional characterisation of 16 Nereocystis-associated bacterial species, comprising 13 Gammaproteobacteria, 2 Flavobacteriia and 1 Actinomycetia. Genome analyses of these isolates highlight metabolisms potentially beneficial to the host, such as B vitamin synthesis and nitrogen retention. Assays revealed that kelp-associated bacteria thrive on amino acids found in high concentrations in the ocean and in the kelp (glutamine and asparagine), generating ammonium that may facilitate host nitrogen acquisition. Multiple isolates have genes indicative of interactions with key elemental cycles in the ocean, including carbon, nitrogen and sulphur. We thus report a collection of kelp-associated microbial isolates that provide functional insight for the future study of kelp–microbe interactions.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impacts of water quality on the amphibian chytrid fungal pathogen: A systematic review 水质对两栖动物糜烂真菌病原体的影响：系统综述。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-22 DOI: 10.1111/1758-2229.13274

Adeline Chew, Matt West, Lee Berger, Laura A. Brannelly

{"title":"The impacts of water quality on the amphibian chytrid fungal pathogen: A systematic review","authors":"Adeline Chew, Matt West, Lee Berger, Laura A. Brannelly","doi":"10.1111/1758-2229.13274","DOIUrl":"10.1111/1758-2229.13274","url":null,"abstract":"The pathogenic fungus Batrachochytrium dendrobatidis has caused declines of amphibians worldwide. Yet our understanding of how water quality influences fungal pathogenicity is limited. Here, we reviewed experimental studies on the effect of water quality on this pathogen to determine which parameters impacted disease dynamics consistently. The strongest evidence for protective effects is salinity which shows strong antifungal properties in hosts at natural levels. Although many fungicides had detrimental effects on the fungal pathogen in vitro, their impact on the host is variable and they can worsen infection outcomes. However, one fungicide, epoxiconazole, reduced disease effects experimentally and likely in the field. While heavy metals are frequently studied, there is weak evidence that they influence infection outcomes. Nitrogen and phosphorous do not appear to impact pathogen growth or infection in the amphibian host. The effects of other chemicals, like pesticides and disinfectants on infection were mostly unclear with mixed results or lacking an in vivo component. Our study shows that water chemistry does impact disease dynamics, but the effects of specific parameters require more investigation. Improving our understanding of how water chemistry influences disease dynamics will help predict the impact of chytridiomycosis, especially in amphibian populations affected by land use changes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-selective microbiota reduction after the elicitation of a seaweed's immune response 激发海藻免疫反应后，非选择性微生物群减少。

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-17 DOI: 10.1111/1758-2229.13268

Jiasui Li, Mahasweta Saha, Marwan E. Majzoub, Teng Yang, Haiyan Chu, Torsten Thomas, Florian Weinberger, Suhelen Egan

{"title":"Non-selective microbiota reduction after the elicitation of a seaweed's immune response","authors":"Jiasui Li, Mahasweta Saha, Marwan E. Majzoub, Teng Yang, Haiyan Chu, Torsten Thomas, Florian Weinberger, Suhelen Egan","doi":"10.1111/1758-2229.13268","DOIUrl":"10.1111/1758-2229.13268","url":null,"abstract":"Pattern-triggered immunity (PTI) is an integral part of the innate immune system of many eukaryotic hosts, assisting in the defence against pathogen invasions. In plants and animals, PTI exerts a selective pressure on the microbiota that can alter community composition. However, the effect of PTI on the microbiota for non-model hosts, including seaweeds, remains unknown. Using quantitative polymerase chain reaction complemented with 16S rRNA gene and transcript amplicon sequencing, this study profiled the impact that PTI of the red seaweed Gracilaria gracilis has on its microbiota. PTI elicitation with agar oligosaccharides resulted in a significant reduction in the number of bacteria (by >75% within 72 h after treatment). However, the PTI elicitation did not cause any significant difference in the community diversity or structure. These findings demonstrated that PTI can be non-selective, and this might help to maintain a stable microbiota by uniformly reducing bacterial loads.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0