Environmental Microbiology Reports最新文献_第8页

More Than a Stick in the Mud: Eelgrass Leaf and Root Bacterial Communities Are Distinct From Those on Physical Mimics 不仅仅是泥里的一根棍子：大叶草的叶子和根的细菌群落不同于那些物理模拟

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-30 DOI: 10.1111/1758-2229.70086

Melissa R. Kardish, John J. Stachowicz

引用次数: 0

Young maize plants impact the bacterial community in Australian cotton-sown vertisol more than agricultural practices 玉米幼苗对澳大利亚棉花垂直土壤细菌群落的影响大于农业实践

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-30 DOI: 10.1111/1758-2229.13322

Luc Dendooven, Daniel Ramírez-Villanueva, Vanessa Romero-Yahuitl, Karla E. Zarco-González, Nilantha Hulugalle, Viliami Heimoana, Nele Verhulst, Bram Govaerts, Yendi E. Navarro-Noya

{"title":"Young maize plants impact the bacterial community in Australian cotton-sown vertisol more than agricultural practices","authors":"Luc Dendooven, Daniel Ramírez-Villanueva, Vanessa Romero-Yahuitl, Karla E. Zarco-González, Nilantha Hulugalle, Viliami Heimoana, Nele Verhulst, Bram Govaerts, Yendi E. Navarro-Noya","doi":"10.1111/1758-2229.13322","DOIUrl":"https://doi.org/10.1111/1758-2229.13322","url":null,"abstract":"Changes in soil characteristics due to varying farming practices can modify the structure of bacterial communities. However, it remains uncertain whether bacterial groups that break down organic material are similarly impacted. We examined changes in the bacterial community by pyrosequencing the 16S rRNA gene when young maize plants, their neutral detergent fibre fraction, or urea were applied to an Australian Vertisol. This soil was managed with either conventional tillage with continuous cotton, minimum tillage with continuous cotton, or a wheat-cotton rotation. The soil organic carbon content was 1.4 times higher in the wheat-cotton rotation than in the conventional tillage with continuous cotton treatment. Approximately 41.6% of the organic carbon was added with maize plants, and 13.1% of the neutral detergent fibre fraction was mineralized after 28 days. The application of young maize plants and the neutral detergent fibre fraction significantly altered the bacterial community and the presumed metabolic functional structure, but urea did not. Many bacterial groups, such as Streptomyces, Nocardioides, and Kribbella, and presumed metabolic functions were enriched by the application of organic material, but less so by urea. We found that a limited number of bacterial groups and presumed metabolic functions were affected in an irrigated Vertisol by the different cotton farming systems, but many were strongly affected by the application of maize plants or its neutral detergent fibre.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13322","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Community-Level Metabolic Shifts Following Land Use Change in the Amazon Rainforest Identified by a Supervised Machine Leaning Approach 基于监督机器学习方法的亚马逊雨林土地利用变化后社区水平代谢变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-23 DOI: 10.1111/1758-2229.70088

Md Abdul Wadud Khan, Brendan J. M. Bohannan, Kyle M. Meyer, Ann M. Womack, Klaus Nüsslein, James P. Grover, Jorge L. Mazza Rodrigues

{"title":"Community-Level Metabolic Shifts Following Land Use Change in the Amazon Rainforest Identified by a Supervised Machine Leaning Approach","authors":"Md Abdul Wadud Khan, Brendan J. M. Bohannan, Kyle M. Meyer, Ann M. Womack, Klaus Nüsslein, James P. Grover, Jorge L. Mazza Rodrigues","doi":"10.1111/1758-2229.70088","DOIUrl":"https://doi.org/10.1111/1758-2229.70088","url":null,"abstract":"The Amazon rainforest has been subjected to high rates of deforestation, mostly for pasturelands, over the last few decades. This change in plant cover is known to alter the soil microbiome and the functions it mediates, but the genomic changes underlying this response are still unresolved. In this study, we used a combination of deep shotgun metagenomics complemented by a supervised machine learning approach to compare the metabolic strategies of tropical soil microbial communities in pristine forests and long-term established pastures in the Amazon. Machine learning-derived metagenome analysis indicated that microbial community structures (bacteria, archaea and viruses) and the composition of protein-coding genes were distinct in each plant cover type environment. Forest and pasture soils had different genomic diversities for the above three taxonomic groups, characterised by their protein-coding genes. These differences in metagenome profiles in soils under forests and pastures suggest that metabolic strategies related to carbohydrate and energy metabolisms were altered at community level. Changes were also consistent with known modifications to the C and N cycles caused by long-term shifts in aboveground vegetation and were also associated with several soil physicochemical properties known to change with land use, such as the C/N ratio, soil temperature and exchangeable acidity. In addition, our analysis reveals that these alterations in land use can also result in changes to the composition and diversity of the soil DNA virome. Collectively, our study indicates that soil microbial communities shift their overall metabolic strategies, driven by genomic alterations observed in pristine forests and long-term established pastures with implications for the C and N cycles.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Community-Level Metabolic Shifts Following Land Use Change in the Amazon Rainforest Identified by a Supervised Machine Leaning Approach 基于监督机器学习方法的亚马逊雨林土地利用变化后社区水平代谢变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-23 DOI: 10.1111/1758-2229.70088

Md Abdul Wadud Khan, Brendan J. M. Bohannan, Kyle M. Meyer, Ann M. Womack, Klaus Nüsslein, James P. Grover, Jorge L. Mazza Rodrigues

{"title":"Community-Level Metabolic Shifts Following Land Use Change in the Amazon Rainforest Identified by a Supervised Machine Leaning Approach","authors":"Md Abdul Wadud Khan, Brendan J. M. Bohannan, Kyle M. Meyer, Ann M. Womack, Klaus Nüsslein, James P. Grover, Jorge L. Mazza Rodrigues","doi":"10.1111/1758-2229.70088","DOIUrl":"https://doi.org/10.1111/1758-2229.70088","url":null,"abstract":"The Amazon rainforest has been subjected to high rates of deforestation, mostly for pasturelands, over the last few decades. This change in plant cover is known to alter the soil microbiome and the functions it mediates, but the genomic changes underlying this response are still unresolved. In this study, we used a combination of deep shotgun metagenomics complemented by a supervised machine learning approach to compare the metabolic strategies of tropical soil microbial communities in pristine forests and long-term established pastures in the Amazon. Machine learning-derived metagenome analysis indicated that microbial community structures (bacteria, archaea and viruses) and the composition of protein-coding genes were distinct in each plant cover type environment. Forest and pasture soils had different genomic diversities for the above three taxonomic groups, characterised by their protein-coding genes. These differences in metagenome profiles in soils under forests and pastures suggest that metabolic strategies related to carbohydrate and energy metabolisms were altered at community level. Changes were also consistent with known modifications to the C and N cycles caused by long-term shifts in aboveground vegetation and were also associated with several soil physicochemical properties known to change with land use, such as the C/N ratio, soil temperature and exchangeable acidity. In addition, our analysis reveals that these alterations in land use can also result in changes to the composition and diversity of the soil DNA virome. Collectively, our study indicates that soil microbial communities shift their overall metabolic strategies, driven by genomic alterations observed in pristine forests and long-term established pastures with implications for the C and N cycles.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Succession of Particle-Attached and Free-Living Microbial Communities in Response to the Degradation of Algal Organic Matter in Lake Taihu, China 太湖藻类有机质降解过程中附着与游离微生物群落的演替

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-20 DOI: 10.1111/1758-2229.70094

Jing Chen, Yongqiang Zhou, Yunlin Zhang, Quanzhong Guo, Shulan Zhang, Guanghuan Ge, Wenting Jin

{"title":"Succession of Particle-Attached and Free-Living Microbial Communities in Response to the Degradation of Algal Organic Matter in Lake Taihu, China","authors":"Jing Chen, Yongqiang Zhou, Yunlin Zhang, Quanzhong Guo, Shulan Zhang, Guanghuan Ge, Wenting Jin","doi":"10.1111/1758-2229.70094","DOIUrl":"https://doi.org/10.1111/1758-2229.70094","url":null,"abstract":"Decomposition of Cyanobacterial blooms frequently occurs in Lake Taihu, releasing various fractions of algal organic matter into the water through cell lysis. These fractions influence the production and consumption of dissolved organic matter, nutrient dynamics, and bacterial succession in the lake. However, the interactions between free-living and particle-attached bacterial communities with different algal organic matter fractions remain poorly understood. Herein, we investigated the effects of two distinct algal organic matter fractions, obtained through a fractionation procedure simulating cyanobacterial bloom collapse, on freshwater bacterial communities. The degradation of both fractions resulted in stage-specific changes in the chemical properties of lake water, which were divided into two distinct stages (labeled Stage I and Stage II). Flavobacteriaceae was dominant in Stage I, whereas Methylophilaceae dominated Stage II. Long-term ecological observations indicated that particle-attached bacteria responded more sensitively to different algal organic matter fractions than free-living bacteria. Compared to the degradation of algal-derived filtrate, the breakdown of algal residual exudative organic matter led to a more complex free-living bacterial community network. These findings provide new insights into the capacity of free-living and particle-attached bacterial communities to utilize different algal organic matter fractions and highlight their roles in aquatic ecosystems during the post-bloom stage.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Succession of Particle-Attached and Free-Living Microbial Communities in Response to the Degradation of Algal Organic Matter in Lake Taihu, China 中国太湖藻类有机物降解过程中颗粒附着和自由生活微生物群落的演替

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-20 DOI: 10.1111/1758-2229.70094

Jing Chen, Yongqiang Zhou, Yunlin Zhang, Quanzhong Guo, Shulan Zhang, Guanghuan Ge, Wenting Jin

{"title":"Succession of Particle-Attached and Free-Living Microbial Communities in Response to the Degradation of Algal Organic Matter in Lake Taihu, China","authors":"Jing Chen, Yongqiang Zhou, Yunlin Zhang, Quanzhong Guo, Shulan Zhang, Guanghuan Ge, Wenting Jin","doi":"10.1111/1758-2229.70094","DOIUrl":"https://doi.org/10.1111/1758-2229.70094","url":null,"abstract":"Decomposition of Cyanobacterial blooms frequently occurs in Lake Taihu, releasing various fractions of algal organic matter into the water through cell lysis. These fractions influence the production and consumption of dissolved organic matter, nutrient dynamics, and bacterial succession in the lake. However, the interactions between free-living and particle-attached bacterial communities with different algal organic matter fractions remain poorly understood. Herein, we investigated the effects of two distinct algal organic matter fractions, obtained through a fractionation procedure simulating cyanobacterial bloom collapse, on freshwater bacterial communities. The degradation of both fractions resulted in stage-specific changes in the chemical properties of lake water, which were divided into two distinct stages (labeled Stage I and Stage II). Flavobacteriaceae was dominant in Stage I, whereas Methylophilaceae dominated Stage II. Long-term ecological observations indicated that particle-attached bacteria responded more sensitively to different algal organic matter fractions than free-living bacteria. Compared to the degradation of algal-derived filtrate, the breakdown of algal residual exudative organic matter led to a more complex free-living bacterial community network. These findings provide new insights into the capacity of free-living and particle-attached bacterial communities to utilize different algal organic matter fractions and highlight their roles in aquatic ecosystems during the post-bloom stage.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum 绿假单胞菌S1Bt23质粒pPNptGreen中绿色荧光蛋白的表达抑制了对黄毒霉的生物防治活性

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-16 DOI: 10.1111/1758-2229.70083

Mercy Akuma, Sylvia Ighem Chi, Renlin Xu, Indira Thapa, Barbara Blackwell, James Tabi Tambong

{"title":"Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum","authors":"Mercy Akuma, Sylvia Ighem Chi, Renlin Xu, Indira Thapa, Barbara Blackwell, James Tabi Tambong","doi":"10.1111/1758-2229.70083","DOIUrl":"https://doi.org/10.1111/1758-2229.70083","url":null,"abstract":"Pseudomonas chlororaphis is a highly effective plant root coloniser and biocontrol agent. To monitor the colonisation of tomato and canola roots, P. chlororaphis S1Bt23 was transformed with the pPNptGreen plasmid encoding for green fluorescent protein (S1Bt23-GFP). Seedling roots inoculated with S1Bt23-GFP were examined after 2 and 5 h using confocal laser fluorescence microscopy. Roots exposed to S1Bt23-GFP showed pronounced biofilm formation around the root surface, and fluorescing cells were localised in the epidermis and metaxylem after 2 and 5 h of inoculation, respectively. The canola roots also showed upward active translocation of the S1Bt23-GFP cells in xylem vessels in real time. S1Bt23-GFP was also evaluated for antagonistic activity against Pythium ultimum. While S1Bt23 WT exhibited 65.70%–71.4% inhibition of radial growth of Py. ultimum, the S1Bt23-GFP strain did not demonstrate any antagonistic effects. Thin layer chromatography and liquid chromatography mass spectrometry analyses of culture extracts of S1Bt23-GFP did not detect phenazines or pyrrolnitrin, antifungal metabolites identified in S1Bt23 wild type. Expressions of phenazine and pyrrolnitrin genes showed no differences in S1Bt23-GFP and wild type. This suggests that the abrogation of these metabolites occurred post-transcriptionally, probably due to a high cellular molecular load in GFP production. This could negatively impact the ecological fitness of S1Bt23-GFP.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Short-Scale Responses: How Pico- and Nanoeukaryotic Plankton Navigate Environmental Variability in a Coastal Upwelling System 揭示短尺度反应：微核和纳米真核浮游生物如何在沿海上升流系统中导航环境变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-16 DOI: 10.1111/1758-2229.70070

María Froján, Marta Muñoz-Colmenero, Isabel G. Teixeira, Belén Arbones, Carmen G. Sotelo, Begoña Correa, Francisco G. Figueiras, Carmen G. Castro

{"title":"Unveiling Short-Scale Responses: How Pico- and Nanoeukaryotic Plankton Navigate Environmental Variability in a Coastal Upwelling System","authors":"María Froján, Marta Muñoz-Colmenero, Isabel G. Teixeira, Belén Arbones, Carmen G. Sotelo, Begoña Correa, Francisco G. Figueiras, Carmen G. Castro","doi":"10.1111/1758-2229.70070","DOIUrl":"https://doi.org/10.1111/1758-2229.70070","url":null,"abstract":"For decades, identifying pico- and nanoeukaryotic plankton has been challenging due to their small size, leaving a significant gap in our knowledge of their composition and dynamics in comparison with their larger counterparts. The advent of molecular techniques unlocked new possibilities for exploring this hidden diversity. We applied metabarcoding targeting the V9 region of 18S rDNA to discern the principal taxonomic groups of pico- and nanoeukaryotes in the Ría de Vigo during the upwelling season. Nanoeukaryotes (NE) exhibit greater diversity compared to picoeukaryotes (PE). Specifically, NE were mainly comprised of nano-sized diatoms and dinoflagellates, many of them uncategorized novel species. Within PE, Syndiniales and Marine Stramenopiles (MAST) were the main components identified. We also captured short-term changes in the biomass and composition of PE an NE, with advection emerging as one of the most significant drivers. Most notably was the ocean inflow of unassigned picoeukaryotes into the Ría, likely driven by the negative circulation during downwelling. Moreover, local grazing within the Ría seems to be important enough to alter NE dynamics, but has a minimal effect over PE. Our findings improve the understanding of the small eukaryotic plankton community in coastal upwelling systems, highlighting a significant potential for novel diversity within these environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Short-Scale Responses: How Pico- and Nanoeukaryotic Plankton Navigate Environmental Variability in a Coastal Upwelling System 揭示短尺度反应：微核和纳米真核浮游生物如何在沿海上升流系统中导航环境变化

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-16 DOI: 10.1111/1758-2229.70070

María Froján, Marta Muñoz-Colmenero, Isabel G. Teixeira, Belén Arbones, Carmen G. Sotelo, Begoña Correa, Francisco G. Figueiras, Carmen G. Castro

{"title":"Unveiling Short-Scale Responses: How Pico- and Nanoeukaryotic Plankton Navigate Environmental Variability in a Coastal Upwelling System","authors":"María Froján, Marta Muñoz-Colmenero, Isabel G. Teixeira, Belén Arbones, Carmen G. Sotelo, Begoña Correa, Francisco G. Figueiras, Carmen G. Castro","doi":"10.1111/1758-2229.70070","DOIUrl":"https://doi.org/10.1111/1758-2229.70070","url":null,"abstract":"For decades, identifying pico- and nanoeukaryotic plankton has been challenging due to their small size, leaving a significant gap in our knowledge of their composition and dynamics in comparison with their larger counterparts. The advent of molecular techniques unlocked new possibilities for exploring this hidden diversity. We applied metabarcoding targeting the V9 region of 18S rDNA to discern the principal taxonomic groups of pico- and nanoeukaryotes in the Ría de Vigo during the upwelling season. Nanoeukaryotes (NE) exhibit greater diversity compared to picoeukaryotes (PE). Specifically, NE were mainly comprised of nano-sized diatoms and dinoflagellates, many of them uncategorized novel species. Within PE, Syndiniales and Marine Stramenopiles (MAST) were the main components identified. We also captured short-term changes in the biomass and composition of PE an NE, with advection emerging as one of the most significant drivers. Most notably was the ocean inflow of unassigned picoeukaryotes into the Ría, likely driven by the negative circulation during downwelling. Moreover, local grazing within the Ría seems to be important enough to alter NE dynamics, but has a minimal effect over PE. Our findings improve the understanding of the small eukaryotic plankton community in coastal upwelling systems, highlighting a significant potential for novel diversity within these environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum 绿假单胞菌S1Bt23质粒pPNptGreen中绿色荧光蛋白的表达抑制了对黄毒霉的生物防治活性

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-16 DOI: 10.1111/1758-2229.70083

Mercy Akuma, Sylvia Ighem Chi, Renlin Xu, Indira Thapa, Barbara Blackwell, James Tabi Tambong

{"title":"Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum","authors":"Mercy Akuma, Sylvia Ighem Chi, Renlin Xu, Indira Thapa, Barbara Blackwell, James Tabi Tambong","doi":"10.1111/1758-2229.70083","DOIUrl":"https://doi.org/10.1111/1758-2229.70083","url":null,"abstract":"Pseudomonas chlororaphis is a highly effective plant root coloniser and biocontrol agent. To monitor the colonisation of tomato and canola roots, P. chlororaphis S1Bt23 was transformed with the pPNptGreen plasmid encoding for green fluorescent protein (S1Bt23-GFP). Seedling roots inoculated with S1Bt23-GFP were examined after 2 and 5 h using confocal laser fluorescence microscopy. Roots exposed to S1Bt23-GFP showed pronounced biofilm formation around the root surface, and fluorescing cells were localised in the epidermis and metaxylem after 2 and 5 h of inoculation, respectively. The canola roots also showed upward active translocation of the S1Bt23-GFP cells in xylem vessels in real time. S1Bt23-GFP was also evaluated for antagonistic activity against Pythium ultimum. While S1Bt23 WT exhibited 65.70%–71.4% inhibition of radial growth of Py. ultimum, the S1Bt23-GFP strain did not demonstrate any antagonistic effects. Thin layer chromatography and liquid chromatography mass spectrometry analyses of culture extracts of S1Bt23-GFP did not detect phenazines or pyrrolnitrin, antifungal metabolites identified in S1Bt23 wild type. Expressions of phenazine and pyrrolnitrin genes showed no differences in S1Bt23-GFP and wild type. This suggests that the abrogation of these metabolites occurred post-transcriptionally, probably due to a high cellular molecular load in GFP production. This could negatively impact the ecological fitness of S1Bt23-GFP.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0