Environmental Microbiology Reports最新文献_第9页

Identifying Potential Geochemical and Microbial Impacts of Hydrogen Storage in a Deep Saline Aquifer 深盐层储氢对地球化学和微生物的潜在影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-15 DOI: 10.1111/1758-2229.70076

Kara A. Tinker, Winston Anthony, Meghan Brandi, Sam Flett, Christopher E. Bagwell, Chuck Smallwood, Ryan Davis, Djuna Gulliver

{"title":"Identifying Potential Geochemical and Microbial Impacts of Hydrogen Storage in a Deep Saline Aquifer","authors":"Kara A. Tinker, Winston Anthony, Meghan Brandi, Sam Flett, Christopher E. Bagwell, Chuck Smallwood, Ryan Davis, Djuna Gulliver","doi":"10.1111/1758-2229.70076","DOIUrl":"https://doi.org/10.1111/1758-2229.70076","url":null,"abstract":"Hydrogen is valuable commodity and a promising energy carrier for variable energy production. Storage of hydrogen may occur through injection of hydrogen or a hydrogen/methane gas blend in subsurface reservoirs. However, the geochemical and biological reactions that may impact the stored hydrogen are not yet understood. Therefore, we collected samples from a deep storage aquifer located in the St. Peter Formation in southern Illinois. The reservoir material was primarily quartz with sulphur and iron deposits, while the major constituents of the fluid were chloride and sulphate. 16S rRNA gene amplicon sequencing revealed a low biomass microbial community that contained no obvious hydrogen-consuming bacteria. Next, we enriched a field sample to increase the biomass and completed a metagenomic analysis, finding a low number of genes present that are associated with hydrogen consumption. Then, we completed a series of reactor experiments under reservoir conditions with 15% H2/85% CH4 gas simulating a short-term hydrogen storage, high withdrawal scenario. We found minimal changes in the geochemistry or microbiology for the reactor experiments. This work suggests that short-term storage may be highly successful, although significant additional work needs to be completed in order to accurately evaluate the risks associated with long-term hydrogen storage scenarios. It is essential we continue to expand our understanding of the dynamics present in saline aquifers and provide new insights into how hydrogen storage may impact underground geological storage environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Potential Geochemical and Microbial Impacts of Hydrogen Storage in a Deep Saline Aquifer 深盐层储氢对地球化学和微生物的潜在影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-15 DOI: 10.1111/1758-2229.70076

Kara A. Tinker, Winston Anthony, Meghan Brandi, Sam Flett, Christopher E. Bagwell, Chuck Smallwood, Ryan Davis, Djuna Gulliver

{"title":"Identifying Potential Geochemical and Microbial Impacts of Hydrogen Storage in a Deep Saline Aquifer","authors":"Kara A. Tinker, Winston Anthony, Meghan Brandi, Sam Flett, Christopher E. Bagwell, Chuck Smallwood, Ryan Davis, Djuna Gulliver","doi":"10.1111/1758-2229.70076","DOIUrl":"https://doi.org/10.1111/1758-2229.70076","url":null,"abstract":"Hydrogen is valuable commodity and a promising energy carrier for variable energy production. Storage of hydrogen may occur through injection of hydrogen or a hydrogen/methane gas blend in subsurface reservoirs. However, the geochemical and biological reactions that may impact the stored hydrogen are not yet understood. Therefore, we collected samples from a deep storage aquifer located in the St. Peter Formation in southern Illinois. The reservoir material was primarily quartz with sulphur and iron deposits, while the major constituents of the fluid were chloride and sulphate. 16S rRNA gene amplicon sequencing revealed a low biomass microbial community that contained no obvious hydrogen-consuming bacteria. Next, we enriched a field sample to increase the biomass and completed a metagenomic analysis, finding a low number of genes present that are associated with hydrogen consumption. Then, we completed a series of reactor experiments under reservoir conditions with 15% H2/85% CH4 gas simulating a short-term hydrogen storage, high withdrawal scenario. We found minimal changes in the geochemistry or microbiology for the reactor experiments. This work suggests that short-term storage may be highly successful, although significant additional work needs to be completed in order to accurately evaluate the risks associated with long-term hydrogen storage scenarios. It is essential we continue to expand our understanding of the dynamics present in saline aquifers and provide new insights into how hydrogen storage may impact underground geological storage environments.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil Microbial Co-Occurrence Networks Across Climate and Land Use Gradient in Southern Italy 意大利南部跨气候和土地利用梯度的土壤微生物共生网络

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-10 DOI: 10.1111/1758-2229.70093

Mohamed Idbella, Giuseppina Iacomino, Ahmed M. Abd-ElGawad, Giuliano Bonanomi

{"title":"Soil Microbial Co-Occurrence Networks Across Climate and Land Use Gradient in Southern Italy","authors":"Mohamed Idbella, Giuseppina Iacomino, Ahmed M. Abd-ElGawad, Giuliano Bonanomi","doi":"10.1111/1758-2229.70093","DOIUrl":"https://doi.org/10.1111/1758-2229.70093","url":null,"abstract":"Despite extensive research on microbiota across land use gradients, it remains unclear if microbial co-occurrence relationships exhibit consistent patterns. Here, we assessed microbial co-occurrence networks of seven natural ecosystems—Quercus ilex forest, Fagus sylvatica forest, Abies alba forest, Mediterranean and mountain grasslands, and subalpine and Mediterranean shrublands—and five agroecosystems, including vineyards, horticulture, greenhouse, a polluted agricultural system, and an arid greenhouse. Soil chemistry, such as pH, organic carbon and total nitrogen, was characterised, and soil microbiota were profiled using high-throughput sequencing from 242 soil samples. Our results revealed that mountain grasslands had the highest organic carbon (86.4 g/kg), while the arid greenhouse had the lowest (6.1 g/kg). Mediterranean grasslands had the lowest pH of 5.79, and vineyards had the highest electrical conductivity of 0.901 dS/m. Notably, natural ecosystem networks exhibited greater modularity, with protected horticulture showing exceptionally the highest (0.937), while intensive agriculture within agroecosystems had a significantly lower modularity of 0.282. Modularity and the number of modules were positively correlated with soil P2O5, while network diameter, path length and clustering coefficient were correlated with soil pH. Additionally, edges and nodes number, average degree and microbial diversity were positively associated with organic carbon and total nitrogen. These findings highlight that natural ecosystems foster more complex and resilient microbial networks, underscoring sustainable land management's importance to preserve soil health and microbial diversity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Texture of Macroplastic Pollution in Streams Alters the Physical Structure and Diversity of Biofilm Communities 河流中巨塑性污染的表面纹理改变了生物膜群落的物理结构和多样性

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-10 DOI: 10.1111/1758-2229.70068

Fabiola Lopez Avila, Krista A. Capps, Raven L. Bier

{"title":"Surface Texture of Macroplastic Pollution in Streams Alters the Physical Structure and Diversity of Biofilm Communities","authors":"Fabiola Lopez Avila, Krista A. Capps, Raven L. Bier","doi":"10.1111/1758-2229.70068","DOIUrl":"https://doi.org/10.1111/1758-2229.70068","url":null,"abstract":"Biofilms can develop on nearly any surface, and in aquatic ecosystems they are essential components of biogeochemical cycles and food webs. Plastic waste in waterways is a new type of surface for biofilm colonisation. To analyse the influence of plastic pollution on the development and diversity of microbial freshwater biofilms that colonised them, we incubated 388 cm2 veneers of high-density polyethylene (HDPE) with two veneer textures, smooth and rough, and tulip tree wood (Liriodendron tulipifera), in three rural headwater streams at the Savannah River Site (Aiken, SC, USA). We collected biofilms from veneers after 14, 28 and 56 days of incubation and analysed 16S rRNA genes and biofilm properties. We found that plastic negatively affected species richness of biofilms compared with wood, but that evenness was greatest on rough textured HDPE. Beta diversity was primarily influenced by stream site. Beta diversity differed more between wood and plastic veneers than with plastic surface texture and became more different over time. Wood had nine times more biomass than rough HDPE and 40 times more biomass than smooth HDPE. Given the projected increase of macroplastic pollution in aquatic ecosystems, our findings emphasise the need to further understand its effects on biofilm characteristics.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil Microbial Co-Occurrence Networks Across Climate and Land Use Gradient in Southern Italy 意大利南部跨气候和土地利用梯度的土壤微生物共生网络

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-10 DOI: 10.1111/1758-2229.70093

Mohamed Idbella, Giuseppina Iacomino, Ahmed M. Abd-ElGawad, Giuliano Bonanomi

{"title":"Soil Microbial Co-Occurrence Networks Across Climate and Land Use Gradient in Southern Italy","authors":"Mohamed Idbella, Giuseppina Iacomino, Ahmed M. Abd-ElGawad, Giuliano Bonanomi","doi":"10.1111/1758-2229.70093","DOIUrl":"https://doi.org/10.1111/1758-2229.70093","url":null,"abstract":"Despite extensive research on microbiota across land use gradients, it remains unclear if microbial co-occurrence relationships exhibit consistent patterns. Here, we assessed microbial co-occurrence networks of seven natural ecosystems—Quercus ilex forest, Fagus sylvatica forest, Abies alba forest, Mediterranean and mountain grasslands, and subalpine and Mediterranean shrublands—and five agroecosystems, including vineyards, horticulture, greenhouse, a polluted agricultural system, and an arid greenhouse. Soil chemistry, such as pH, organic carbon and total nitrogen, was characterised, and soil microbiota were profiled using high-throughput sequencing from 242 soil samples. Our results revealed that mountain grasslands had the highest organic carbon (86.4 g/kg), while the arid greenhouse had the lowest (6.1 g/kg). Mediterranean grasslands had the lowest pH of 5.79, and vineyards had the highest electrical conductivity of 0.901 dS/m. Notably, natural ecosystem networks exhibited greater modularity, with protected horticulture showing exceptionally the highest (0.937), while intensive agriculture within agroecosystems had a significantly lower modularity of 0.282. Modularity and the number of modules were positively correlated with soil P2O5, while network diameter, path length and clustering coefficient were correlated with soil pH. Additionally, edges and nodes number, average degree and microbial diversity were positively associated with organic carbon and total nitrogen. These findings highlight that natural ecosystems foster more complex and resilient microbial networks, underscoring sustainable land management's importance to preserve soil health and microbial diversity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Texture of Macroplastic Pollution in Streams Alters the Physical Structure and Diversity of Biofilm Communities 河流中巨塑性污染的表面纹理改变了生物膜群落的物理结构和多样性

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-04-10 DOI: 10.1111/1758-2229.70068

Fabiola Lopez Avila, Krista A. Capps, Raven L. Bier

{"title":"Surface Texture of Macroplastic Pollution in Streams Alters the Physical Structure and Diversity of Biofilm Communities","authors":"Fabiola Lopez Avila, Krista A. Capps, Raven L. Bier","doi":"10.1111/1758-2229.70068","DOIUrl":"https://doi.org/10.1111/1758-2229.70068","url":null,"abstract":"Biofilms can develop on nearly any surface, and in aquatic ecosystems they are essential components of biogeochemical cycles and food webs. Plastic waste in waterways is a new type of surface for biofilm colonisation. To analyse the influence of plastic pollution on the development and diversity of microbial freshwater biofilms that colonised them, we incubated 388 cm2 veneers of high-density polyethylene (HDPE) with two veneer textures, smooth and rough, and tulip tree wood (Liriodendron tulipifera), in three rural headwater streams at the Savannah River Site (Aiken, SC, USA). We collected biofilms from veneers after 14, 28 and 56 days of incubation and analysed 16S rRNA genes and biofilm properties. We found that plastic negatively affected species richness of biofilms compared with wood, but that evenness was greatest on rough textured HDPE. Beta diversity was primarily influenced by stream site. Beta diversity differed more between wood and plastic veneers than with plastic surface texture and became more different over time. Wood had nine times more biomass than rough HDPE and 40 times more biomass than smooth HDPE. Given the projected increase of macroplastic pollution in aquatic ecosystems, our findings emphasise the need to further understand its effects on biofilm characteristics.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Harmful Cyanobacterium Microcystis aeruginosa Differently Affects the Growth Rate and Photosynthetic Efficiency of Several Species of Marine Phytoplankton 有害蓝藻铜绿微囊藻对几种海洋浮游植物生长速率和光合效率的影响不同

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-03-28 DOI: 10.1111/1758-2229.70091

Na Yun Park, Hyun Soo Choi, Sang Uk Kang, An Suk Lim

{"title":"The Harmful Cyanobacterium Microcystis aeruginosa Differently Affects the Growth Rate and Photosynthetic Efficiency of Several Species of Marine Phytoplankton","authors":"Na Yun Park, Hyun Soo Choi, Sang Uk Kang, An Suk Lim","doi":"10.1111/1758-2229.70091","DOIUrl":"https://doi.org/10.1111/1758-2229.70091","url":null,"abstract":"Microcystis aeruginosa is a major cyanobacterium that can secrete toxins, such as microcystin, and causes harmful algal blooms. Despite extensive research on the effects of microcystins on other organisms, research on how introduced M. aeruginosa into estuaries affects marine phytoplankton is scarce. In this study, the effect of M. aeruginosa on the growth of seven representative marine phytoplankton species that inhabit estuaries was determined. The marine phytoplankton species differed in their responses to M. aeruginosa; of the seven species, the growth rate of Akashiwo sanguinea was the most affected by M. aeruginosa cells and filtrate. Moreover, our results revealed that M. aeruginosa affected the growth rate and photosynthetic efficiency of A. sanguinea in a density- and time-dependent manner. Our findings suggest that M. aeruginosa may alter the phytoplankton community structure in estuarine ecosystems.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Harmful Cyanobacterium Microcystis aeruginosa Differently Affects the Growth Rate and Photosynthetic Efficiency of Several Species of Marine Phytoplankton 有害蓝藻铜绿微囊藻对几种海洋浮游植物生长速率和光合效率的影响不同

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-03-28 DOI: 10.1111/1758-2229.70091

Na Yun Park, Hyun Soo Choi, Sang Uk Kang, An Suk Lim

{"title":"The Harmful Cyanobacterium Microcystis aeruginosa Differently Affects the Growth Rate and Photosynthetic Efficiency of Several Species of Marine Phytoplankton","authors":"Na Yun Park, Hyun Soo Choi, Sang Uk Kang, An Suk Lim","doi":"10.1111/1758-2229.70091","DOIUrl":"https://doi.org/10.1111/1758-2229.70091","url":null,"abstract":"Microcystis aeruginosa is a major cyanobacterium that can secrete toxins, such as microcystin, and causes harmful algal blooms. Despite extensive research on the effects of microcystins on other organisms, research on how introduced M. aeruginosa into estuaries affects marine phytoplankton is scarce. In this study, the effect of M. aeruginosa on the growth of seven representative marine phytoplankton species that inhabit estuaries was determined. The marine phytoplankton species differed in their responses to M. aeruginosa; of the seven species, the growth rate of Akashiwo sanguinea was the most affected by M. aeruginosa cells and filtrate. Moreover, our results revealed that M. aeruginosa affected the growth rate and photosynthetic efficiency of A. sanguinea in a density- and time-dependent manner. Our findings suggest that M. aeruginosa may alter the phytoplankton community structure in estuarine ecosystems.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa 群体感应调控的sRNA Lrs1参与了铜绿假单胞菌对低铁的适应

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-03-27 DOI: 10.1111/1758-2229.70090

Dimitra Panagiotopoulou, Natalia Romo Catalán, Max Wilcox, Nigel Halliday, Paolo Pantalone, James Lazenby, Miguel Cámara, Stephan Heeb

{"title":"The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa","authors":"Dimitra Panagiotopoulou, Natalia Romo Catalán, Max Wilcox, Nigel Halliday, Paolo Pantalone, James Lazenby, Miguel Cámara, Stephan Heeb","doi":"10.1111/1758-2229.70090","DOIUrl":"https://doi.org/10.1111/1758-2229.70090","url":null,"abstract":"Iron is an essential nutrient for microbial growth. The opportunistic pathogen Pseudomonas aeruginosa can survive under diverse conditions, including iron-depleted environments with the aid of small non-coding RNAs (sRNAs). P. aeruginosa also uses three quorum sensing (QS) systems: Las, Rhl and Pqs, to coordinate virulence and infection establishment at the population level. The aim of this study is to investigate the role of the sRNA Lrs1, the gene of which is positioned within the promoter of the Pqs biosynthetic operon pqsABCDE. Transcriptomics and phenotypic assays indicate that Lrs1 downregulates the production of the siderophore pyochelin but not pyoverdine, and that lrs1 regulation itself is dependent on iron availability. Although Lrs1 has been implicated in a positive feedback loop with the transcriptional regulator LasR in the strain PA14, the present findings indicate that this is not the case in PAO1-L in the tested conditions. Transcription of Lrs1 is dependent on quorum sensing, predominantly on RhlR with an auxiliary effect by PqsE. Furthermore, the Pqs system and phenazine production are modulated by Lrs1 only under iron limitation. This study identifies Lrs1 as a new QS-dependent post-transcriptional regulator in low iron, highlighting its importance in environmental adaptation in P. aeruginosa.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa 群体感应调控的sRNA Lrs1参与了铜绿假单胞菌对低铁的适应

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-03-27 DOI: 10.1111/1758-2229.70090

Dimitra Panagiotopoulou, Natalia Romo Catalán, Max Wilcox, Nigel Halliday, Paolo Pantalone, James Lazenby, Miguel Cámara, Stephan Heeb

{"title":"The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa","authors":"Dimitra Panagiotopoulou, Natalia Romo Catalán, Max Wilcox, Nigel Halliday, Paolo Pantalone, James Lazenby, Miguel Cámara, Stephan Heeb","doi":"10.1111/1758-2229.70090","DOIUrl":"https://doi.org/10.1111/1758-2229.70090","url":null,"abstract":"Iron is an essential nutrient for microbial growth. The opportunistic pathogen Pseudomonas aeruginosa can survive under diverse conditions, including iron-depleted environments with the aid of small non-coding RNAs (sRNAs). P. aeruginosa also uses three quorum sensing (QS) systems: Las, Rhl and Pqs, to coordinate virulence and infection establishment at the population level. The aim of this study is to investigate the role of the sRNA Lrs1, the gene of which is positioned within the promoter of the Pqs biosynthetic operon pqsABCDE. Transcriptomics and phenotypic assays indicate that Lrs1 downregulates the production of the siderophore pyochelin but not pyoverdine, and that lrs1 regulation itself is dependent on iron availability. Although Lrs1 has been implicated in a positive feedback loop with the transcriptional regulator LasR in the strain PA14, the present findings indicate that this is not the case in PAO1-L in the tested conditions. Transcription of Lrs1 is dependent on quorum sensing, predominantly on RhlR with an auxiliary effect by PqsE. Furthermore, the Pqs system and phenazine production are modulated by Lrs1 only under iron limitation. This study identifies Lrs1 as a new QS-dependent post-transcriptional regulator in low iron, highlighting its importance in environmental adaptation in P. aeruginosa.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0