The Decrease of Soil Microbial Community Diversity and Network Complexity Results in the Increase of Soil-Borne Diseases With Monocultural Years in Greenhouse Tomato Production Systems

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Jing Hu, Li Wan, Yafang Wang, Kuai Dai, Klaus Butterbach-Bahl, Shan Lin
{"title":"The Decrease of Soil Microbial Community Diversity and Network Complexity Results in the Increase of Soil-Borne Diseases With Monocultural Years in Greenhouse Tomato Production Systems","authors":"Jing Hu,&nbsp;Li Wan,&nbsp;Yafang Wang,&nbsp;Kuai Dai,&nbsp;Klaus Butterbach-Bahl,&nbsp;Shan Lin","doi":"10.1111/1758-2229.70165","DOIUrl":null,"url":null,"abstract":"<p>The excessive use of water and fertiliser, along with long-term monocultivation in greenhouse vegetable fields, has led to a decrease in soil quality and an imbalance in the soil microflora, which may contribute to worsening soil-borne diseases. To examine the associations between the soil microbial community composition and disease-suppressing microorganisms under different years of cultivation shifting from croplands to greenhouses, we collected soil samples from fields continuously planted with tomatoes for 5, 10 and 20 years, as well as from nearby maize–wheat fields (MW). The soil physicochemical properties, microbial community composition, putative plant pathogens and putative antagonistic bacteria were analysed. The results indicated that (1) the diversity and richness of soil bacteria and fungi significantly decreased with longer cultivation years; (2) bacterial and fungal diversity indices were negatively associated with soil nutrient content and positively associated with soil pH and NO<sub>3</sub><sup>−</sup>-N content, the latter being identified as the main factor contributing to the decline in microbial diversity; (3) the complexity of the soil bacterial network initially increased, peaking after 5 years of continuous monoculture, and then decreased, whereas fungal network complexity gradually declined over time; (4) the relative abundance of putative pathogens such as <i>Fusarium</i>, <i>Alternaria</i> and <i>Cladosporium</i> (associated with tomato wilt and leaf mould) increased significantly with longer cultivation, whereas the relative abundance of the bacterial genera associated with putative antagonism <i>Bacillus</i>, <i>Paenibacillus</i> and <i>Streptomyces</i> decreased. In conclusion, after 10 years of continuous monocultivation, a marked reduction in soil microbial diversity and putative antagonistic bacteria was observed, along with an increase in putative pathogenic fungi. These changes likely contributed to the worsening of soil-borne diseases, threatening the sustainability of greenhouse vegetable production.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70165","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.70165","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The excessive use of water and fertiliser, along with long-term monocultivation in greenhouse vegetable fields, has led to a decrease in soil quality and an imbalance in the soil microflora, which may contribute to worsening soil-borne diseases. To examine the associations between the soil microbial community composition and disease-suppressing microorganisms under different years of cultivation shifting from croplands to greenhouses, we collected soil samples from fields continuously planted with tomatoes for 5, 10 and 20 years, as well as from nearby maize–wheat fields (MW). The soil physicochemical properties, microbial community composition, putative plant pathogens and putative antagonistic bacteria were analysed. The results indicated that (1) the diversity and richness of soil bacteria and fungi significantly decreased with longer cultivation years; (2) bacterial and fungal diversity indices were negatively associated with soil nutrient content and positively associated with soil pH and NO3-N content, the latter being identified as the main factor contributing to the decline in microbial diversity; (3) the complexity of the soil bacterial network initially increased, peaking after 5 years of continuous monoculture, and then decreased, whereas fungal network complexity gradually declined over time; (4) the relative abundance of putative pathogens such as Fusarium, Alternaria and Cladosporium (associated with tomato wilt and leaf mould) increased significantly with longer cultivation, whereas the relative abundance of the bacterial genera associated with putative antagonism Bacillus, Paenibacillus and Streptomyces decreased. In conclusion, after 10 years of continuous monocultivation, a marked reduction in soil microbial diversity and putative antagonistic bacteria was observed, along with an increase in putative pathogenic fungi. These changes likely contributed to the worsening of soil-borne diseases, threatening the sustainability of greenhouse vegetable production.

Abstract Image

随着栽培年限的增加,温室番茄土壤微生物群落多样性和网络复杂性的降低导致土壤病害的增加
过度使用水和肥料,加上温室菜地长期单一栽培,导致土壤质量下降和土壤微生物群失衡,这可能导致土传疾病恶化。为了研究从农田到温室不同栽培年限下土壤微生物群落组成与疾病抑制微生物之间的关系,我们收集了连续种植番茄5年、10年和20年的田地以及附近的玉米小麦田(MW)的土壤样本。分析了土壤理化性质、微生物群落组成、推定植物病原菌和推定拮抗菌。结果表明:(1)随着栽培年限的延长,土壤细菌和真菌的多样性和丰富度显著降低;(2)细菌和真菌多样性指数与土壤养分含量呈负相关,与土壤pH和NO3−-N含量呈正相关,后者被认为是导致微生物多样性下降的主要因素;(3)土壤细菌网络复杂性呈上升趋势,在连续单作5年后达到峰值,随后下降,真菌网络复杂性随着时间的推移逐渐下降;(4)随着栽培时间的延长,与番茄枯萎病和叶霉病相关的镰刀菌、Alternaria和Cladosporium等推定病原菌的相对丰度显著增加,而与推定拮抗菌Bacillus、Paenibacillus和Streptomyces相关的细菌属的相对丰度则显著降低。综上所述,经过10年的连续单一栽培,观察到土壤微生物多样性和推定的拮抗细菌显著减少,同时推定的致病真菌增加。这些变化可能导致土壤传播疾病的恶化,威胁到温室蔬菜生产的可持续性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信