Environmental Microbiology Reports最新文献_第10页

Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems 不同地下生态系统中候选念珠菌核心代谢功能的差异表达

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-03 DOI: 10.1111/1758-2229.70096

Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst

{"title":"Differential Expression of Core Metabolic Functions in Candidatus Altiarchaeum Inhabiting Distinct Subsurface Ecosystems","authors":"Sarah P. Esser, Victoria Turzynski, Julia Plewka, Julia Nuy, Carrie J. Moore, Indra Banas, André R. Soares, Janey Lee, Tanja Woyke, Alexander J. Probst","doi":"10.1111/1758-2229.70096","DOIUrl":"https://doi.org/10.1111/1758-2229.70096","url":null,"abstract":"Candidatus Altiarchaea are widespread across aquatic subsurface ecosystems and possess a highly conserved core genome, yet adaptations of this core genome to different biotic and abiotic factors based on gene expression remain unknown. Here, we investigated the metatranscriptome of two Ca. Altiarchaeum populations that thrive in two substantially different subsurface ecosystems. In Crystal Geyser, a high-CO2 groundwater system in the USA, Ca. Altiarchaeum crystalense co-occurs with the symbiont Ca. Huberiarchaeum crystalense, while in the Muehlbacher sulfidic spring in Germany, an artesian spring high in sulfide concentration, Ca. A. hamiconexum is heavily infected with viruses. We here mapped metatranscriptome reads against their genomes to analyse the in situ expression profile of their core genomes. Out of 537 shared gene clusters, 331 were functionally annotated and 130 differed significantly in expression between the two sites. Main differences were related to genes involved in cell defence like CRISPR-Cas, virus defence, replication, transcription and energy and carbon metabolism. Our results demonstrate that altiarchaeal populations in the subsurface are likely adapted to their environment while influenced by other biological entities that tamper with their core metabolism. We consequently posit that viruses and symbiotic interactions can be major energy sinks for organisms in the deep biosphere.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation 铁限制下硼库木香SK2基因表达、蛋白质合成及表观遗传修饰的综合分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-02 DOI: 10.1111/1758-2229.70106

Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva

{"title":"Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation","authors":"Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva","doi":"10.1111/1758-2229.70106","DOIUrl":"https://doi.org/10.1111/1758-2229.70106","url":null,"abstract":"This study aimed to understand the genetic and molecular mechanisms enabling Alcanivorax borkumensis SK2, a hydrocarbonoclastic marine bacterium, to thrive under iron-limited conditions. Using SMRT PacBio whole-genome sequencing, Illumina total RNA sequencing, and proteomics analysis, we examined the strain's response to iron-rich and iron-depleted media. Despite minimal impact on growth, significant changes in gene expression were observed when using n-tetradecane or acetate under iron limitation. Iron scarcity, depending on the carbon source, affects energy metabolism, membrane transport, lipid metabolism, stress-adaptive responses, and siderophore synthesis. We identified several methyltransferases (MTases) in the studied genome, including RS14230, which is a part of a fully functional restriction-modification (RM) system causing bipartite cytosine methylation and DNA cleavage at AgGCcT sites. Another MTase, RS09425, controls bipartite adenine methylation at GaTNNNNNGtGG motifs; however, no restriction activity at these motifs has been detected. Many epigenetically modified nucleotides lacked canonical motifs, possibly due to MTase byproducts. Notably, non-canonical modifications were statistically associated with transcriptional start sites and gene regulation, suggesting an indirect role in transcription via DNA conformation changes and its accessibility to MTases near actively transcribed genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation 铁限制下硼库木香SK2基因表达、蛋白质合成及表观遗传修饰的综合分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-02 DOI: 10.1111/1758-2229.70106

Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva

{"title":"Integrated Analysis of Gene Expression, Protein Synthesis, and Epigenetic Modifications in Alcanivorax borkumensis SK2 Under Iron Limitation","authors":"Francesco Smedile, Renata Denaro, Francesca Crisafi, Domenico Giosa, Giuseppe D'Auria, Manuel Ferrer, Riccardo Rosselli, Martin S. Staege, Michail M. Yakimov, Laura Giuliano, Oleg N. Reva","doi":"10.1111/1758-2229.70106","DOIUrl":"https://doi.org/10.1111/1758-2229.70106","url":null,"abstract":"This study aimed to understand the genetic and molecular mechanisms enabling Alcanivorax borkumensis SK2, a hydrocarbonoclastic marine bacterium, to thrive under iron-limited conditions. Using SMRT PacBio whole-genome sequencing, Illumina total RNA sequencing, and proteomics analysis, we examined the strain's response to iron-rich and iron-depleted media. Despite minimal impact on growth, significant changes in gene expression were observed when using n-tetradecane or acetate under iron limitation. Iron scarcity, depending on the carbon source, affects energy metabolism, membrane transport, lipid metabolism, stress-adaptive responses, and siderophore synthesis. We identified several methyltransferases (MTases) in the studied genome, including RS14230, which is a part of a fully functional restriction-modification (RM) system causing bipartite cytosine methylation and DNA cleavage at AgGCcT sites. Another MTase, RS09425, controls bipartite adenine methylation at GaTNNNNNGtGG motifs; however, no restriction activity at these motifs has been detected. Many epigenetically modified nucleotides lacked canonical motifs, possibly due to MTase byproducts. Notably, non-canonical modifications were statistically associated with transcriptional start sites and gene regulation, suggesting an indirect role in transcription via DNA conformation changes and its accessibility to MTases near actively transcribed genes.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms 生物可利用的溶解有机碳是河流生物膜中磷动态的关键调节因子

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-01 DOI: 10.1111/1758-2229.70115

Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere

{"title":"Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms","authors":"Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere","doi":"10.1111/1758-2229.70115","DOIUrl":"https://doi.org/10.1111/1758-2229.70115","url":null,"abstract":"Phosphorus (P) dynamics at the sediment–water interface of aquatic ecosystems are receiving increasing attention due to their implications for water quality. P uptake by microbial biofilms can serve as a mechanism to control and mitigate the risk of eutrophication. Microbial biofilms capture P both intracellularly and extracellularly. While the significance of extracellular P entrapment in biofilms in engineered systems has recently been established, little is known about its dynamics in aquatic ecosystems. Current research on eutrophication control predominantly emphasises nitrogen, phosphorus or nitrogen-phosphorus ratio-based approaches, often overlooking the potential indirect influence of bioavailable dissolved organic carbon (DOC) on P uptake by heterotrophic microorganisms. In this study, we tested the effect of bioavailable DOC on P entrapment patterns in biofilms and in biofilm P-regulation mechanisms such as polyphosphate accumulation and alkaline phosphatase activity in semi-natural flow-through experimental flumes. Our results show that intracellular P entrapment is limited by bioavailable DOC, while extracellular P entrapment is independent of bioavailable DOC and has the potential to offset intracellular P saturation. We further demonstrate that DOC bioavailability influences benthic P cycling and that its implications extend into critical areas of ecosystem functioning such as river self-purification, competitive resource utilisation and organic P cycling.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms 生物可利用的溶解有机碳是河流生物膜中磷动态的关键调节因子

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-06-01 DOI: 10.1111/1758-2229.70115

Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere

{"title":"Bioavailable Dissolved Organic Carbon Serves as a Key Regulator of Phosphorus Dynamics in Stream Biofilms","authors":"Nuria Perujo, Daniel Graeber, Patrick Fink, Lola Neuert, Nergui Sunjidmaa, Markus Weitere","doi":"10.1111/1758-2229.70115","DOIUrl":"https://doi.org/10.1111/1758-2229.70115","url":null,"abstract":"Phosphorus (P) dynamics at the sediment–water interface of aquatic ecosystems are receiving increasing attention due to their implications for water quality. P uptake by microbial biofilms can serve as a mechanism to control and mitigate the risk of eutrophication. Microbial biofilms capture P both intracellularly and extracellularly. While the significance of extracellular P entrapment in biofilms in engineered systems has recently been established, little is known about its dynamics in aquatic ecosystems. Current research on eutrophication control predominantly emphasises nitrogen, phosphorus or nitrogen-phosphorus ratio-based approaches, often overlooking the potential indirect influence of bioavailable dissolved organic carbon (DOC) on P uptake by heterotrophic microorganisms. In this study, we tested the effect of bioavailable DOC on P entrapment patterns in biofilms and in biofilm P-regulation mechanisms such as polyphosphate accumulation and alkaline phosphatase activity in semi-natural flow-through experimental flumes. Our results show that intracellular P entrapment is limited by bioavailable DOC, while extracellular P entrapment is independent of bioavailable DOC and has the potential to offset intracellular P saturation. We further demonstrate that DOC bioavailability influences benthic P cycling and that its implications extend into critical areas of ecosystem functioning such as river self-purification, competitive resource utilisation and organic P cycling.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vibrio cholerae in Water Environments: A Systematic Review and Meta-Analysis 水环境中的霍乱弧菌：系统综述和荟萃分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-05-29 DOI: 10.1111/1758-2229.70103

Aaron Awere-Duodu, Onyansaniba K. Ntim, Eric S. Donkor

{"title":"Vibrio cholerae in Water Environments: A Systematic Review and Meta-Analysis","authors":"Aaron Awere-Duodu, Onyansaniba K. Ntim, Eric S. Donkor","doi":"10.1111/1758-2229.70103","DOIUrl":"https://doi.org/10.1111/1758-2229.70103","url":null,"abstract":"Vibrio cholerae is a water-borne pathogen transmitted via the faecal-oral route, with water being a major vehicle for infection. The pathogen has caused seven pandemics in history, with contaminated water identified as the infection source. Seasonal outbreaks, claiming approximately 21,000–143,000 lives yearly, are facilitated by contaminated water environments. This systematic review, therefore, determined the prevalence of V. cholerae in water environments. A comprehensive literature search was conducted in PubMed, Web of Science, and SCOPUS. After the screening process, 87 articles were included in our study. RStudio version 4.3.3 was used in conducting our meta-analysis with the data subjected to the random-effects model. The included articles were from 38 countries, which spanned 6 continents. The prevalences of V. cholerae in water environments were as follows: drinking water (15.69%), untreated sewage (57.26%), treated sewage (95.18%), surface water (41.95%), groundwater (26.23%), and others (20.81%). Continental prevalence revealed the following: Australia (85.00%), North America (66.60%), Africa (42.07), South America (39.32%), Asia (29.28%), and Europe (24.48%). There is a high prevalence of V. cholerae in water environments. More effective water treatment methods are needed to drastically reduce its prevalence to insignificant levels, especially in treated drinking water.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vibrio cholerae in Water Environments: A Systematic Review and Meta-Analysis 水环境中的霍乱弧菌：系统综述和荟萃分析

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-05-29 DOI: 10.1111/1758-2229.70103

Aaron Awere-Duodu, Onyansaniba K. Ntim, Eric S. Donkor

{"title":"Vibrio cholerae in Water Environments: A Systematic Review and Meta-Analysis","authors":"Aaron Awere-Duodu, Onyansaniba K. Ntim, Eric S. Donkor","doi":"10.1111/1758-2229.70103","DOIUrl":"https://doi.org/10.1111/1758-2229.70103","url":null,"abstract":"Vibrio cholerae is a water-borne pathogen transmitted via the faecal-oral route, with water being a major vehicle for infection. The pathogen has caused seven pandemics in history, with contaminated water identified as the infection source. Seasonal outbreaks, claiming approximately 21,000–143,000 lives yearly, are facilitated by contaminated water environments. This systematic review, therefore, determined the prevalence of V. cholerae in water environments. A comprehensive literature search was conducted in PubMed, Web of Science, and SCOPUS. After the screening process, 87 articles were included in our study. RStudio version 4.3.3 was used in conducting our meta-analysis with the data subjected to the random-effects model. The included articles were from 38 countries, which spanned 6 continents. The prevalences of V. cholerae in water environments were as follows: drinking water (15.69%), untreated sewage (57.26%), treated sewage (95.18%), surface water (41.95%), groundwater (26.23%), and others (20.81%). Continental prevalence revealed the following: Australia (85.00%), North America (66.60%), Africa (42.07), South America (39.32%), Asia (29.28%), and Europe (24.48%). There is a high prevalence of V. cholerae in water environments. More effective water treatment methods are needed to drastically reduce its prevalence to insignificant levels, especially in treated drinking water.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pectobacterium versatile β-Lactamase Contributes to Soft Rot Pectobacteriaceae (SRP) Community Diversity During Potato Infection 马铃薯侵染过程中，多用途β-内酰胺酶对软腐pectobacteraceae （SRP）群落多样性的影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-05-26 DOI: 10.1111/1758-2229.70111

Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, Jacques Pédron, Marie-Anne Barny

{"title":"Pectobacterium versatile β-Lactamase Contributes to Soft Rot Pectobacteriaceae (SRP) Community Diversity During Potato Infection","authors":"Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, Jacques Pédron, Marie-Anne Barny","doi":"10.1111/1758-2229.70111","DOIUrl":"https://doi.org/10.1111/1758-2229.70111","url":null,"abstract":"Little is known about the role of antibiotics in microbial ecosystems in the absence of clinical antibiotic pressure. The soft rot Pectobacteriaceae (SRP) species complex comprises 37 bacterial species that are collectively responsible for severe plant decay in many crops. Within this complex, Pectobacterium versatile strains harbour the BlaPEC-1 β-lactamase. The aim of our work was to analyse the role of BlaPEC-1 during plant infection. To this end, two blaPEC-1-deleted strains were compared with their wild-type counterparts in vitro and in mono- or mixed infections of potato tubers with different SRP strains. In vitro, BlaPEC-1 enables P. versatile to resist ampicillin or the carbapenem produced by Pectobacterium brasiliense. In mono-infections of potato tubers, blaPEC-1-deleted strains were not affected in virulence, fitness, or association with bacterial commensals. In mixed infections, BlaPEC-1 was required for the coexistence of P. versatile with the carbapenem-producing strain and was necessary to rescue carbapenem-sensitive strains both in vitro and in planta. Protection was observed even if both the blaPEC-1 gene was repressed and the BlaPEC1-expressing bacteria were a minority in the symptoms. These results indicate that BlaPEC-1 exerts a true β-lactamase function during the infection process and acts as a public good of the SRP species complex to maintain SRP strain diversity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting Microbial Consortia for Tebuthiuron Degradation in Agricultural Soils: An Alternative Bayesian-Driven Colorimetric Protocol 农业土壤中丁硫脲降解的生物勘探微生物群落：另一种贝叶斯驱动比色法方案

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-05-26 DOI: 10.1111/1758-2229.70109

Letícia Barbosa Jorososki, Bruno Rafael de Almeida Moreira, Victor Hugo Cruz, Yanca Araujo Frias, Paulo Renato Matos Lopes

{"title":"Bioprospecting Microbial Consortia for Tebuthiuron Degradation in Agricultural Soils: An Alternative Bayesian-Driven Colorimetric Protocol","authors":"Letícia Barbosa Jorososki, Bruno Rafael de Almeida Moreira, Victor Hugo Cruz, Yanca Araujo Frias, Paulo Renato Matos Lopes","doi":"10.1111/1758-2229.70109","DOIUrl":"https://doi.org/10.1111/1758-2229.70109","url":null,"abstract":"Herbicides impact, particularly tebuthiuron (TBT), on agroecosystems and surrounding environments had been documented in scientific literature. This study investigated the hypothesis that areas exposed to TBT, a prevalent herbicide in Brazil's sugarcane crops, might harbour microbial consortia capable of degrading this compound, assessed through a Bayesian-based colorimetric method. Soil samples from plant cane (pC), characterised by lower organic matter on the surface, and first-cut ratoon cane (rC) fields, with higher organic matter due to crop residues deposition, were collected for analysis. Colorimetric assays with DCPIP (2,6-Dichlorophenolindophenol) were performed with microorganisms isolated from these fields to evaluate their TBT-degradation capacity. In an ELISA microplate, absorbance was measured at 600 nm as DCPIP is a redox indicator. Results confirmed the degradation potential of soil microbial consortia, particularly from rC samples, as evidenced by reduced absorbance relative to the control. The data deviated from the expected sigmoidal pattern, necessitating an alternative data interpretation method. A Bayesian factor estimation approach for kernel density curves of the logarithmic response ratio proved effective for handling non-sigmoidal spectrophotometric data. The findings offered valuable insights into TBT-degrading microorganisms and introduced an alternative analytical tool for interpreting variable data, potentially aiding in the development of bio-based remediation strategies.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pectobacterium versatile β-Lactamase Contributes to Soft Rot Pectobacteriaceae (SRP) Community Diversity During Potato Infection 马铃薯侵染过程中，多用途β-内酰胺酶对软腐pectobacteraceae （SRP）群落多样性的影响

IF 3.6 4区生物学

Environmental Microbiology Reports Pub Date : 2025-05-26 DOI: 10.1111/1758-2229.70111

Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, Jacques Pédron, Marie-Anne Barny

{"title":"Pectobacterium versatile β-Lactamase Contributes to Soft Rot Pectobacteriaceae (SRP) Community Diversity During Potato Infection","authors":"Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, Jacques Pédron, Marie-Anne Barny","doi":"10.1111/1758-2229.70111","DOIUrl":"https://doi.org/10.1111/1758-2229.70111","url":null,"abstract":"Little is known about the role of antibiotics in microbial ecosystems in the absence of clinical antibiotic pressure. The soft rot Pectobacteriaceae (SRP) species complex comprises 37 bacterial species that are collectively responsible for severe plant decay in many crops. Within this complex, Pectobacterium versatile strains harbour the BlaPEC-1 β-lactamase. The aim of our work was to analyse the role of BlaPEC-1 during plant infection. To this end, two blaPEC-1-deleted strains were compared with their wild-type counterparts in vitro and in mono- or mixed infections of potato tubers with different SRP strains. In vitro, BlaPEC-1 enables P. versatile to resist ampicillin or the carbapenem produced by Pectobacterium brasiliense. In mono-infections of potato tubers, blaPEC-1-deleted strains were not affected in virulence, fitness, or association with bacterial commensals. In mixed infections, BlaPEC-1 was required for the coexistence of P. versatile with the carbapenem-producing strain and was necessary to rescue carbapenem-sensitive strains both in vitro and in planta. Protection was observed even if both the blaPEC-1 gene was repressed and the BlaPEC1-expressing bacteria were a minority in the symptoms. These results indicate that BlaPEC-1 exerts a true β-lactamase function during the infection process and acts as a public good of the SRP species complex to maintain SRP strain diversity.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0