Environmental Microbiology Reports最新文献_第10页

Metagenomic analysis of microbial consortia native to the Amazon, Highlands, and Galapagos regions of Ecuador with potential for wastewater remediation 对厄瓜多尔亚马逊河、高原和加拉帕戈斯地区原生微生物群进行元基因组分析，分析其在废水修复方面的潜力

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI: 10.1111/1758-2229.13272

Juan José Guadalupe, Miguel Pazmiño-Vela, Gabriela Pozo, Wendy Vernaza, Valeria Ochoa-Herrera, Maria de Lourdes Torres, Andres F. Torres

{"title":"Metagenomic analysis of microbial consortia native to the Amazon, Highlands, and Galapagos regions of Ecuador with potential for wastewater remediation","authors":"Juan José Guadalupe, Miguel Pazmiño-Vela, Gabriela Pozo, Wendy Vernaza, Valeria Ochoa-Herrera, Maria de Lourdes Torres, Andres F. Torres","doi":"10.1111/1758-2229.13272","DOIUrl":"https://doi.org/10.1111/1758-2229.13272","url":null,"abstract":"Native microbial consortia have been proposed for biological wastewater treatment, but their diversity and function remain poorly understood. This study investigated three native microalgae-bacteria consortia collected from the Amazon, Highlands, and Galapagos regions of Ecuador to assess their metagenomes and wastewater remediation potential. The consortia were evaluated for 12 days under light (LC) and continuous dark conditions (CDC) to measure their capacity for nutrient and organic matter removal from synthetic wastewater (SWW). Overall, all three consortia demonstrated higher nutrient removal efficiencies under LC than CDC, with the Amazon and Galapagos consortia outperforming the Highlands consortium in nutrient removal capabilities. Despite differences in α- and β-diversity, microbial species diversity within and between consortia did not directly correlate with their nutrient removal capabilities. However, all three consortia were enriched with core taxonomic groups associated with wastewater remediation activities. Our analyses further revealed higher abundances for nutrient removing microorganisms in the Amazon and Galapagos consortia compared with the Highland consortium. Finally, this study also uncovered the contribution of novel microbial groups that enhance wastewater bioremediation processes. These groups have not previously been reported as part of the core microbial groups commonly found in wastewater communities, thereby highlighting the potential of investigating microbial consortia isolated from ecosystems of megadiverse countries like Ecuador.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and composition of microbial communities in the water column from Southern Gulf of Mexico and detection of putative hydrocarbon-degrading microorganisms 墨西哥湾南部水体微生物群落的结构和组成以及推定碳氢化合物降解微生物的检测

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI: 10.1111/1758-2229.13264

Sonia S. Valencia-Agami, Daniel Cerqueda-García, Abril M. Gamboa-Muñoz, M. Leopoldina Aguirre-Macedo, José Q. García-Maldonado

{"title":"Structure and composition of microbial communities in the water column from Southern Gulf of Mexico and detection of putative hydrocarbon-degrading microorganisms","authors":"Sonia S. Valencia-Agami, Daniel Cerqueda-García, Abril M. Gamboa-Muñoz, M. Leopoldina Aguirre-Macedo, José Q. García-Maldonado","doi":"10.1111/1758-2229.13264","DOIUrl":"https://doi.org/10.1111/1758-2229.13264","url":null,"abstract":"This study assessed the bacterioplankton community and its relationship with environmental variables, including total petroleum hydrocarbon (TPH) concentration, in the Yucatan shelf area of the Southern Gulf of Mexico. Beta diversity analyses based on 16S rRNA sequences indicated variations in the bacterioplankton community structure among sampling sites. PERMANOVA indicated that these variations could be mainly related to changes in depth (5 to 180 m), dissolved oxygen concentration (2.06 to 5.93 mg L−1), and chlorophyll-a concentration (0.184 to 7.65 mg m3). Moreover, SIMPER and one-way ANOVA analyses showed that the shifts in the relative abundances of Synechococcus and Prochlorococcus were related to changes in microbial community composition and chlorophyll-a values. Despite the low TPH content measured in the studied sites (0.01 to 0.86 μL L−1), putative hydrocarbon-degrading bacteria such as Alteromonas, Acinetobacter, Balneola, Erythrobacter, Oleibacter, Roseibacillus, and the MWH-UniP1 aquatic group were detected. The relatively high copy number of the alkB gene detected in the water column by qPCR and the enrichment of hydrocarbon-degrading bacteria obtained during lab crude oil tests exhibited the potential of bacterioplankton communities from the Yucatan shelf to respond to potential hydrocarbon impacts in this important area of the Gulf Mexico.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and composition of microbial communities in the water column from Southern Gulf of Mexico and detection of putative hydrocarbon-degrading microorganisms 墨西哥湾南部水体微生物群落的结构和组成以及推定碳氢化合物降解微生物的检测

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI: 10.1111/1758-2229.13264

Sonia S. Valencia-Agami, Daniel Cerqueda-García, Abril M. Gamboa-Muñoz, M. Leopoldina Aguirre-Macedo, José Q. García-Maldonado

{"title":"Structure and composition of microbial communities in the water column from Southern Gulf of Mexico and detection of putative hydrocarbon-degrading microorganisms","authors":"Sonia S. Valencia-Agami, Daniel Cerqueda-García, Abril M. Gamboa-Muñoz, M. Leopoldina Aguirre-Macedo, José Q. García-Maldonado","doi":"10.1111/1758-2229.13264","DOIUrl":"https://doi.org/10.1111/1758-2229.13264","url":null,"abstract":"This study assessed the bacterioplankton community and its relationship with environmental variables, including total petroleum hydrocarbon (TPH) concentration, in the Yucatan shelf area of the Southern Gulf of Mexico. Beta diversity analyses based on 16S rRNA sequences indicated variations in the bacterioplankton community structure among sampling sites. PERMANOVA indicated that these variations could be mainly related to changes in depth (5 to 180 m), dissolved oxygen concentration (2.06 to 5.93 mg L−1), and chlorophyll-a concentration (0.184 to 7.65 mg m3). Moreover, SIMPER and one-way ANOVA analyses showed that the shifts in the relative abundances of Synechococcus and Prochlorococcus were related to changes in microbial community composition and chlorophyll-a values. Despite the low TPH content measured in the studied sites (0.01 to 0.86 μL L−1), putative hydrocarbon-degrading bacteria such as Alteromonas, Acinetobacter, Balneola, Erythrobacter, Oleibacter, Roseibacillus, and the MWH-UniP1 aquatic group were detected. The relatively high copy number of the alkB gene detected in the water column by qPCR and the enrichment of hydrocarbon-degrading bacteria obtained during lab crude oil tests exhibited the potential of bacterioplankton communities from the Yucatan shelf to respond to potential hydrocarbon impacts in this important area of the Gulf Mexico.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro interactions between Bradyrhizobium spp. and Tuber magnatum mycelium 巴西根瘤菌属与大块茎菌丝体之间的体外相互作用

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI: 10.1111/1758-2229.13271

Simone Graziosi, Federico Puliga, Mirco Iotti, Antonella Amicucci, Alessandra Zambonelli

{"title":"In vitro interactions between Bradyrhizobium spp. and Tuber magnatum mycelium","authors":"Simone Graziosi, Federico Puliga, Mirco Iotti, Antonella Amicucci, Alessandra Zambonelli","doi":"10.1111/1758-2229.13271","DOIUrl":"https://doi.org/10.1111/1758-2229.13271","url":null,"abstract":"Tuber magnatum is the most expensive truffle, but its large-scale cultivation is still a challenge compared to other valuable Tuber species. T. magnatum mycelium has never been grown profitably until now, which has led to difficulties to studying it in vitro. This study describes beneficial interactions between T. magnatum mycelium and never before described bradyrhizobia, which allows the in vitro growth of T. magnatum mycelium. Three T. magnatum strains were co-isolated on modified Woody Plant Medium (mWPM) with aerobic bacteria and characterised through microscopic observations. The difficulties of growing alone both partners, bacteria and T. magnatum mycelium, on mWPM demonstrated the reciprocal dependency. Three bacterial isolates for each T. magnatum strain were obtained and molecularly characterised by sequencing the 16S rRNA, glnII, recA and nifH genes. Phylogenetic analyses showed that all nine bacterial strains were distributed among five subclades included in a new monophyletic lineage belonging to the Bradyrhizobium genus within the Bradyrhizobium jicamae supergroup. The nifH genes were detected in all bacterial isolates, suggesting nitrogen-fixing capacities. This is the first report of consistent T. magnatum mycelium growth in vitro conditions. It has important implications for the development of new technologies in white truffle cultivation and for further studies on T. magnatum biology and genetics.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro interactions between Bradyrhizobium spp. and Tuber magnatum mycelium 巴西根瘤菌属与大块茎菌丝体之间的体外相互作用

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI: 10.1111/1758-2229.13271

Simone Graziosi, Federico Puliga, Mirco Iotti, Antonella Amicucci, Alessandra Zambonelli

{"title":"In vitro interactions between Bradyrhizobium spp. and Tuber magnatum mycelium","authors":"Simone Graziosi, Federico Puliga, Mirco Iotti, Antonella Amicucci, Alessandra Zambonelli","doi":"10.1111/1758-2229.13271","DOIUrl":"https://doi.org/10.1111/1758-2229.13271","url":null,"abstract":"Tuber magnatum is the most expensive truffle, but its large-scale cultivation is still a challenge compared to other valuable Tuber species. T. magnatum mycelium has never been grown profitably until now, which has led to difficulties to studying it in vitro. This study describes beneficial interactions between T. magnatum mycelium and never before described bradyrhizobia, which allows the in vitro growth of T. magnatum mycelium. Three T. magnatum strains were co-isolated on modified Woody Plant Medium (mWPM) with aerobic bacteria and characterised through microscopic observations. The difficulties of growing alone both partners, bacteria and T. magnatum mycelium, on mWPM demonstrated the reciprocal dependency. Three bacterial isolates for each T. magnatum strain were obtained and molecularly characterised by sequencing the 16S rRNA, glnII, recA and nifH genes. Phylogenetic analyses showed that all nine bacterial strains were distributed among five subclades included in a new monophyletic lineage belonging to the Bradyrhizobium genus within the Bradyrhizobium jicamae supergroup. The nifH genes were detected in all bacterial isolates, suggesting nitrogen-fixing capacities. This is the first report of consistent T. magnatum mycelium growth in vitro conditions. It has important implications for the development of new technologies in white truffle cultivation and for further studies on T. magnatum biology and genetics.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov. 新产玫瑰黄素链霉菌 Streptomyces berlinensis sp.

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-04-23 DOI: 10.1111/1758-2229.13266

Jimmy Jonathan Liunardo, Sebastien Messerli, Ann-Kathrin Gregotsch, Sonja Lang, Kerstin Schlosser, Christian Rückert-Reed, Tobias Busche, Jörn Kalinowski, Martin Zischka, Philipp Weller, Imen Nouioui, Meina Neumann-Schaal, Chandra Risdian, Joachim Wink, Matthias Mack

{"title":"Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov.","authors":"Jimmy Jonathan Liunardo, Sebastien Messerli, Ann-Kathrin Gregotsch, Sonja Lang, Kerstin Schlosser, Christian Rückert-Reed, Tobias Busche, Jörn Kalinowski, Martin Zischka, Philipp Weller, Imen Nouioui, Meina Neumann-Schaal, Chandra Risdian, Joachim Wink, Matthias Mack","doi":"10.1111/1758-2229.13266","DOIUrl":"https://doi.org/10.1111/1758-2229.13266","url":null,"abstract":"The Gram-positive bacteria Streptomyces davaonensis and Streptomyces cinnabarinus have been the only organisms known to produce roseoflavin, a riboflavin (vitamin B2) derived red antibiotic. Using a selective growth medium and a phenotypic screening, we were able to isolate a novel roseoflavin producer from a German soil sample. The isolation procedure was repeated twice, that is, the same strain could be isolated from the same location in Berlin 6 months and 12 months after its first isolation. Whole genome sequencing of the novel roseoflavin producer revealed an unusual chromosomal arrangement and the deposited genome sequence of the new isolate (G + C content of 71.47%) contains 897 genes per inverted terminal repeat, 6190 genes in the core and 107 genes located on an illegitimate terminal end. We identified the roseoflavin biosynthetic genes rosA, rosB and rosC and an unusually high number of riboflavin biosynthetic genes. Overexpression of rosA, rosB and rosC in Escherichia coli and enzyme assays confirmed their predicted functions in roseoflavin biosynthesis. A full taxonomic analysis revealed that the isolate represents a previously unknown Streptomyces species and we propose the name Streptomyces berlinensis sp. nov. for this roseoflavin producer.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov. 新产玫瑰黄素链霉菌 Streptomyces berlinensis sp.

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-04-23 DOI: 10.1111/1758-2229.13266

Jimmy Jonathan Liunardo, Sebastien Messerli, Ann-Kathrin Gregotsch, Sonja Lang, Kerstin Schlosser, Christian Rückert-Reed, Tobias Busche, Jörn Kalinowski, Martin Zischka, Philipp Weller, Imen Nouioui, Meina Neumann-Schaal, Chandra Risdian, Joachim Wink, Matthias Mack

{"title":"Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov.","authors":"Jimmy Jonathan Liunardo, Sebastien Messerli, Ann-Kathrin Gregotsch, Sonja Lang, Kerstin Schlosser, Christian Rückert-Reed, Tobias Busche, Jörn Kalinowski, Martin Zischka, Philipp Weller, Imen Nouioui, Meina Neumann-Schaal, Chandra Risdian, Joachim Wink, Matthias Mack","doi":"10.1111/1758-2229.13266","DOIUrl":"https://doi.org/10.1111/1758-2229.13266","url":null,"abstract":"The Gram-positive bacteria Streptomyces davaonensis and Streptomyces cinnabarinus have been the only organisms known to produce roseoflavin, a riboflavin (vitamin B2) derived red antibiotic. Using a selective growth medium and a phenotypic screening, we were able to isolate a novel roseoflavin producer from a German soil sample. The isolation procedure was repeated twice, that is, the same strain could be isolated from the same location in Berlin 6 months and 12 months after its first isolation. Whole genome sequencing of the novel roseoflavin producer revealed an unusual chromosomal arrangement and the deposited genome sequence of the new isolate (G + C content of 71.47%) contains 897 genes per inverted terminal repeat, 6190 genes in the core and 107 genes located on an illegitimate terminal end. We identified the roseoflavin biosynthetic genes rosA, rosB and rosC and an unusually high number of riboflavin biosynthetic genes. Overexpression of rosA, rosB and rosC in Escherichia coli and enzyme assays confirmed their predicted functions in roseoflavin biosynthesis. A full taxonomic analysis revealed that the isolate represents a previously unknown Streptomyces species and we propose the name Streptomyces berlinensis sp. nov. for this roseoflavin producer.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial endophytic community composition varies by hemp cultivar in commercially sourced seed 商业种子中不同大麻品种的细菌内生群落组成各不相同

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-04-22 DOI: 10.1111/1758-2229.13259

Jack Davies, Sarah Hawkins, Ana Winters, Kerrie Farrar

{"title":"Bacterial endophytic community composition varies by hemp cultivar in commercially sourced seed","authors":"Jack Davies, Sarah Hawkins, Ana Winters, Kerrie Farrar","doi":"10.1111/1758-2229.13259","DOIUrl":"https://doi.org/10.1111/1758-2229.13259","url":null,"abstract":"The seed-endophytic bacterial community is a potentially beneficial and heritable fraction of the plant microbiome. Its utilization as a sustainable crop improvement strategy could be especially valuable for species such as hemp, where production is being scaled up and new challenges will be faced in managing crop productivity and health. However, little is known about the makeup and variation of the hemp seed microbiome. This study profiled the endophytic bacterial communities harboured by 16 hemp cultivars sourced from commercial suppliers in Europe. A 16S rDNA amplicon sequencing approach identified 917 amplicon sequence variants across samples. Taxonomic classification of sequences revealed 4 phyla and 87 genera to be represented in the dataset. Several genera were widespread while some were specific to one or a few cultivars. Flavobacterium, Pseudomonas, and Pantoea were notable in their high overall abundance and prevalence, but community composition was variable and no one taxon was universally abundant, suggesting a high degree of flexibility in community assembly. Taxonomic composition and alpha diversity differed among cultivars, though further work is required to understand the relative influence of hemp genetic factors on community structure. The taxonomic profiles presented here can be used to inform further work investigating the functional characteristics and potential plant-growth-promoting traits of seed-borne bacteria in hemp.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140633732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial endophytic community composition varies by hemp cultivar in commercially sourced seed 商业种子中不同大麻品种的细菌内生群落组成各不相同

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-04-22 DOI: 10.1111/1758-2229.13259

Jack Davies, Sarah Hawkins, Ana Winters, Kerrie Farrar

{"title":"Bacterial endophytic community composition varies by hemp cultivar in commercially sourced seed","authors":"Jack Davies, Sarah Hawkins, Ana Winters, Kerrie Farrar","doi":"10.1111/1758-2229.13259","DOIUrl":"https://doi.org/10.1111/1758-2229.13259","url":null,"abstract":"The seed-endophytic bacterial community is a potentially beneficial and heritable fraction of the plant microbiome. Its utilization as a sustainable crop improvement strategy could be especially valuable for species such as hemp, where production is being scaled up and new challenges will be faced in managing crop productivity and health. However, little is known about the makeup and variation of the hemp seed microbiome. This study profiled the endophytic bacterial communities harboured by 16 hemp cultivars sourced from commercial suppliers in Europe. A 16S rDNA amplicon sequencing approach identified 917 amplicon sequence variants across samples. Taxonomic classification of sequences revealed 4 phyla and 87 genera to be represented in the dataset. Several genera were widespread while some were specific to one or a few cultivars. Flavobacterium, Pseudomonas, and Pantoea were notable in their high overall abundance and prevalence, but community composition was variable and no one taxon was universally abundant, suggesting a high degree of flexibility in community assembly. Taxonomic composition and alpha diversity differed among cultivars, though further work is required to understand the relative influence of hemp genetic factors on community structure. The taxonomic profiles presented here can be used to inform further work investigating the functional characteristics and potential plant-growth-promoting traits of seed-borne bacteria in hemp.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140633731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological exploration of the Cueva del Viento lava tube system in Tenerife, Canary Islands 加那利群岛特内里费岛 Cueva del Viento 熔岩管系统的微生物学探索

IF 3.3 4区生物学

Environmental Microbiology Reports Pub Date : 2024-04-21 DOI: 10.1111/1758-2229.13245

Sara Gutierrez-Patricio, Jorge R. Osman, José Luis Gonzalez-Pimentel, Valme Jurado, Leonila Laiz, Alfredo Laínez Concepción, Cesareo Saiz-Jimenez, Ana Zélia Miller

{"title":"Microbiological exploration of the Cueva del Viento lava tube system in Tenerife, Canary Islands","authors":"Sara Gutierrez-Patricio, Jorge R. Osman, José Luis Gonzalez-Pimentel, Valme Jurado, Leonila Laiz, Alfredo Laínez Concepción, Cesareo Saiz-Jimenez, Ana Zélia Miller","doi":"10.1111/1758-2229.13245","DOIUrl":"https://doi.org/10.1111/1758-2229.13245","url":null,"abstract":"Cueva del Viento, located in the Canary Islands, Spain, is the Earth's sixth-longest lava tube, spanning 18,500 m, and was formed approximately 27,000 years ago. This complex volcanic cave system is characterized by a unique geomorphology, featuring an intricate network of galleries. Despite its geological significance, the geomicrobiology of Cueva del Viento remains largely unexplored. This study employed a combination of culture-dependent techniques and metabarcoding data analysis to gain a comprehensive understanding of the cave's microbial diversity. The 16S rRNA gene metabarcoding approach revealed that the coloured microbial mats (yellow, red and white) coating the cave walls are dominated by the phyla Actinomycetota, Pseudomonadota and Acidobacteriota. Of particular interest is the high relative abundance of the genus Crossiella, which is involved in urease-mediated biomineralization processes, along with the presence of genera associated with nitrogen cycling, such as Nitrospira. Culture-dependent techniques provided insights into the morphological characteristics of the isolated species and their potential metabolic activities, particularly for the strains Streptomyces spp., Paenarthrobacter sp. and Pseudomonas spp. Our findings underscore the potential of Cueva del Viento as an ideal environment for studying microbial diversity and for the isolation and characterization of novel bacterial species of biotechnological interest.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0