Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, Jacques Pédron, Marie-Anne Barny
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引用次数: 0
Abstract
Little is known about the role of antibiotics in microbial ecosystems in the absence of clinical antibiotic pressure. The soft rot Pectobacteriaceae (SRP) species complex comprises 37 bacterial species that are collectively responsible for severe plant decay in many crops. Within this complex, Pectobacterium versatile strains harbour the BlaPEC-1 β-lactamase. The aim of our work was to analyse the role of BlaPEC-1 during plant infection. To this end, two blaPEC-1-deleted strains were compared with their wild-type counterparts in vitro and in mono- or mixed infections of potato tubers with different SRP strains. In vitro, BlaPEC-1 enables P. versatile to resist ampicillin or the carbapenem produced by Pectobacterium brasiliense. In mono-infections of potato tubers, blaPEC-1-deleted strains were not affected in virulence, fitness, or association with bacterial commensals. In mixed infections, BlaPEC-1 was required for the coexistence of P. versatile with the carbapenem-producing strain and was necessary to rescue carbapenem-sensitive strains both in vitro and in planta. Protection was observed even if both the blaPEC-1 gene was repressed and the BlaPEC1-expressing bacteria were a minority in the symptoms. These results indicate that BlaPEC-1 exerts a true β-lactamase function during the infection process and acts as a public good of the SRP species complex to maintain SRP strain diversity.
期刊介绍：
The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side.
Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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