Insights Into the Mondia Whitei Microbiome Across Geographic Regions in Eastern Africa

IF 2.7 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Expedito Olimi, Regina Wuggenig, Carolina Lobato, Samuel Bickel, Peter Kusstatscher, Wisnu Adi Wicaksono, Angelika Battisti, Danny Coyne, John Adriko, Tomislav Cernava, Gabriele Berg
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Abstract

Mondia whitei (Hook.f.) Skeels is an essential medicinal plant in African societies. However, little is known about the plant's metabolome and its microbiota. Here, we examine the root endosphere and rhizosphere from five locations in Uganda using high-throughput amplicon sequencing, qPCR and multifactorial modelling. Root metabolite profiles obtained with GC/LC–MS were comprehensively catalogued through a deep literature survey using 516 sources. Plant roots were inhabited by microbiota ranging between 50 and 500 ASVs, also with an average microbial abundance of 1011 gene (16SrRNA or ITS) copies per gram. The microbiota was dominated by Gammaproteobacteria, Alphaproteobacteria and Actinobacteria, as well as Sordariomycetes, Dothideomycota, Eurotiomycetes and Agaricomycetes. We identified that a major portion of the microbiome (i.e., 45%–70%) was potentially transferred from the rhizosphere into the roots. Therefore, the root microbiota showed a strong location-specific microbial and metabolite fingerprint. Fraxin, 4-methoxy-benzaldehyde, monobutyl phthalate, 2-hydroxy-4-methoxybenzaldehyde, and scopoletin were among the 86 compounds found in plant roots that were strongly correlated with the root microbiota. Moreover, the identified plant compounds have been shown to mediate microbe, plant, and animal interactions. Our research advances the research frontiers of endangered African herbal plants, through providing insights into the microbiome and potential compounds of M. whitei, a medicinal plant used in sub-Saharan Africa.

Abstract Image

东非不同地理区域的白月牙菌群研究
白月牙(Hook.f.)骷髅是非洲社会必不可少的药用植物。然而,人们对这种植物的代谢组和微生物群知之甚少。在这里,我们使用高通量扩增子测序、qPCR和多因子模型研究了乌干达五个地点的根内圈和根际。通过对516个来源的深入文献调查,对GC/ LC-MS获得的根代谢物谱进行了全面编目。植物根部的微生物群分布在50 ~ 500个asv之间,平均微生物丰度为每克1011个基因(16SrRNA或ITS)拷贝。微生物群以γ -变形菌、α -变形菌和放线菌为主,以及Sordariomycetes、Dothideomycota、eurotiomyetes和Agaricomycetes。我们发现大部分微生物组(即45%-70%)可能从根际转移到根中。因此,根系微生物群显示出强烈的位置特异性微生物和代谢物指纹。在植物根系中发现了86种与根系微生物群密切相关的化合物,其中包括Fraxin、4-甲氧基苯甲醛、邻苯二甲酸一丁酯、2-羟基-4-甲氧基苯甲醛和东莨菪碱。此外,已鉴定的植物化合物已被证明介导微生物,植物和动物的相互作用。我们的研究通过提供对撒哈拉以南非洲使用的药用植物M. whitei的微生物组和潜在化合物的见解,推进了濒危非洲草本植物的研究前沿。
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来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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