Journal of Integrative Plant Biology最新文献

{"title":"Novel effector HYPB1 of cotton bollworm (Helicoverpa armigera) inhibits biosynthesis of plant secondary metabolites and promotes feeding by targeting cotton dirigent protein GhDIR15.","authors":"Yaxin Wang, Chuanying Zhu, Ying Wang, Peng Han, Xueke Li, Gefei Chen, Ying Wang, Muna Alariqi, Zhongping Xu, Qiongqiong Wang, Fuqiu Wang, Yan Zhang, Lianlian Che, Amjad Hussain, Xinhui Nie, Wei Gao, Xianlong Zhang, Longfu Zhu, Shuangxia Jin","doi":"10.1111/jipb.70270","DOIUrl":"10.1111/jipb.70270","url":null,"abstract":"<p><p>Herbivore effectors play central roles in plant-insect interactions; yet, their molecular targets and modes of action remain poorly defined. Here, we performed data-independent acquisition proteomic profiling of oral secretions from cotton bollworm (Helicoverpa armigera) larvae fed on an artificial diet and four cotton cultivars. A total of 212 proteins were identified, including 39 differentially expressed proteins and 13 candidate effectors. Based on secretion characteristics and evolutionary features, six venom protein-related candidates were selected for functional validation. Transgenic cotton plants overexpressing these genes were generated, and feeding assays demonstrated that three independent 35S:PESD3 lines and three 35S:HYPB1 lines significantly enhanced bollworm performance relative to wild-type cotton. Further analyses showed that HYPB1 and PESD3 can be secreted into cotton tissues through mechanical wounds. Among these candidates, HYPB1 showed typical structural and evolutionary characteristics of venom-related proteins. Multiple complementary protein-protein interaction assays demonstrated that HYPB1 physically interacts with the cotton dirigent protein GhDIR15. Silencing of GhDIR15 via virus-induced gene silencing reduced cotton resistance to H. armigera and was accompanied by decreased lignin accumulation and reduced phenolic metabolite levels, indicating suppression of the cell wall-associated defense pathway. Together, these results identify HYPB1 as a previously uncharacterized effector that promotes bollworm feeding by targeting GhDIR15 and suppressing lignin biosynthesis, thereby further compromising cell wall-mediated defense. Although PESD3 also promoted bollworm performance in transgenic cotton, its underlying mechanism requires further investigation. This work provides mechanistic insight into how H. armigera manipulates host secondary metabolism to attenuate plant defense.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3473-3489"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A group of TCP transcription factors is a missing link in strigolactone signaling.","authors":"Yi Huang, Lumei Bi, Yongshuang Huang, Jinlan Liu, Lin Wang, Fang Qiu, Yuehua Wang, Li Chen, Meng Zhang, Ruifeng Yao","doi":"10.1111/jipb.70281","DOIUrl":"10.1111/jipb.70281","url":null,"abstract":"<p><p>Strigolactones (SLs) are plant-specialized butenolide signaling molecules, recognized as endogenous plant hormones, that control plant development and environmental adaptation. In Arabidopsis (Arabidopsis thaliana), the repressor D53-like SMXLs regulate the expression of a vast number of genes in an EAR-motif-dependent manner to mediate SL signaling. However, it remains unclear how the SMXLs are recruited to specific genes and implement unique functions in vivo. Based on chromatin co-distribution analysis, we constructed a chromatin co-localization map of SMXL6 with 108 transcription factors. Among the candidate transcription factors, the Class II TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family member TCP4 shows the highest frequency of chromatin co-localization with SMXL6. SMXL6 and TCP4 co‑localize at the promoter regions of 18 SL-induced SMXL6 target genes (SISGs), including BRC1. We confirmed that TCP4 interacts with SMXL6 and can bind directly to these co‑localized sites. The loss of CIN-TCPs function reduces the hormone responsiveness of the SL-induced genes. Introducing the tcp3/4/10 into SL‑deficient mutants restored the BRC1 expression to a level exceeding that of the wild type. However, the branching phenotype of the SL‑deficient mutant was only partially rescued, suggesting a limited role for BRC1 in SL‑mediated branching control and implicating the involvement of additional factors. An unexpected finding was that tcp3/4/10 rescued the dwarf phenotype of the SL‑deficient mutants, providing an opportunity to elucidate the mechanisms underlying SL‑regulated plant height. These findings demonstrate that TCP4 mediates SMXL6 chromatin recruitment during SL signaling, and provide a new understanding of how SMXL6 participates in SL signaling-mediated gene expression and plant development.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3426-3439"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Base editing in rice using nuclease-deactivated CRISPR/Cas-SF01.","authors":"Meng Jia, Yangqing Xie, Chuang Wei, Hongzhi Wang, Li Xue, Chunyu Zou, Jian-Kang Zhu, Mugui Wang","doi":"10.1111/jipb.70265","DOIUrl":"10.1111/jipb.70265","url":null,"abstract":"<p><p>Adenine and cytosine base editing using dCas-SF01 and the 35S-CmYLCV-U6 composite promoter successfully introduced targeted base substitutions at multiple loci in rice, with average editing efficiency of 33.3%. Using the protospacer adjacent motif (PAM)-relaxed SF01-IKRR variant enabled base editing using 5'-NTN PAMs in rice.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3181-3183"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns.","authors":"Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi","doi":"10.1111/jipb.70249","DOIUrl":"10.1111/jipb.70249","url":null,"abstract":"<p><p>Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools for precise genome editing in plants. Conventionally, such base editors are built upon the CRISPR-Cas9 systems where Cas9 nickases are used. To expand the base editing scope and minimize off-target effects, base editors derived from the CRISPR-Cas12a systems are desired. However, the use of deactivated Cas12a (dCas12a) in such base editors constrains the editing activity, preventing the wide use of Cas12a base editors for plant research and trait development. In this study, we demonstrate the use of an ABE based on the efficient LbCas12a-RRV variant to introduce herbicide-resistant mutations in OsACCase in rice. To improve Cas12a CBEs and ABEs, we inserted introns into the coding sequence of dLbCas12a-RRV. This intron-containing Cas12a-CBE shows substantial improvement in editing efficiency in rice, compared to the intron-less counterparts. By contrast, the improvement of ABE with the intron-containing dLbCas12a-RRV is very limited, partly due to the already high baseline editing efficiency of the intron-less dLbCas12a-RRV ABE. Testing of these base editors in poplar shows elevated C-to-T base editing by dLbCas12a-RRV-intron-CBE. For A-to-G editing, ABEs built upon dLbCas12a-RV and dLbCas12a-RRV variants showed significant improvement over ABEs derived from wild-type LbCas12a and the ttLbCas12a variant. The addition of introns to dLbCas12a-RRV does not further improve the base editing efficiency. With whole genome sequencing in rice, we evaluated genome editing specificities with these improved Cas12a base editors. Our analyses show that both intron-containing Cas12a CBE and ABE barely introduce guide RNA-dependent off-target mutations. However, they can generate guide RNA-independent off-target mutations, which are likely attributed to the high enzymatic activities of the deaminases. Collectively, our study demonstrates the successful use of a Cas12a base editor for trait development and reports improved Cas12a CBEs and ABEs for precise base editing in plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3243-3259"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Cas12i3 system enables precise OsAUX3 editing for rice grain improvement.","authors":"Rui Zhang, Xu Tang, Xinruolan Yang, Yong Zhang","doi":"10.1111/jipb.70337","DOIUrl":"10.1111/jipb.70337","url":null,"abstract":"<p><p>An optimized Cas12i3 genome-editing system enables highly efficient and predictable editing of regulatory sequences in rice. Precise promoter engineering fine-tunes gene expression, improves grain size, and enhances production potential, demonstrating a powerful new approach for crop improvement through targeted regulation rather than gene disruption.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3187-3189"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing precisely boosts salt-tolerant japonica rice yield.","authors":"Mengmeng Zhou, Qihao Sun, Jian Sun, Jiaoyan An, Yanpeng Lv, Cui Li, Yunlong Lu, Jiyue Qiao, Xiaoming Zheng, Qian Qian","doi":"10.1111/jipb.70313","DOIUrl":"10.1111/jipb.70313","url":null,"abstract":"<p><p>Gene editing of three key genes, G PROTEIN GAMMA SUBUNIT, CYTOKININ OXIDASE, AND IDEAL PLANT ARCHITECTURE 1, improved the salt-tolerant japonica rice variety Lansheng increased yield while maintaining its original salt tolerance and quality.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3295-3297"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A GmOIL2 allele separates fertility and seed traits in soybean.","authors":"Ying Chen, Jing-Tian Wang, De-Hui Yuan, Yi-Lin Zeng, Xiao-Shuang Ma, Xiu-Ting Liang, Qiu-Yun Jiang, Yi-Yi Xia, Jian-Fang Zuo, Yuan-Ming Zhang","doi":"10.1111/jipb.70345","DOIUrl":"10.1111/jipb.70345","url":null,"abstract":"<p><p>A CRISPR-derived 36-bp in-frame deletion in GmOIL2 maintains fertility and seed weight while altering seed-related traits. This allele provides a genetic resource for improving soybean quality without affecting seed weight.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3301-3303"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient CRISPR/Cas-SF01 genome editing tools with high editing efficiency in allotetraploid oilseed rape.","authors":"Mengyu Hao, Meili Zhou, Fei Pan, Tiantian Liu, Yilin Li, Nan Su, Ayub Ashfaq, Miaoying Song, Hui Wang, Wenxiang Wang, Jia Liu, Chao Li, Li Fu, Ping He, Qiong Hu, Desheng Mei, Hongtao Cheng","doi":"10.1111/jipb.70221","DOIUrl":"10.1111/jipb.70221","url":null,"abstract":"<p><p>CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 has been widely utilized for plant genome editing, but the protospacer adjacent motif (PAM) requirement limits its editing scope. CRISPR/Cas12i3 belongs to the type-VI Cas system that has gained extensive attention due to its smaller size and less restricted canonical TTN PAM sequence. In this study, we explored the newly developed Cas-SF01 system (Cas12i3 variant) for genome editing in oilseed rape. We established an efficient protoplast transformation system in oilseed rape to compare editing efficiency between Cas-SF01 and Cas9. Cas-SF01 shows cleavage activities at the tested 5'-TTN-3' PAM sites with editing outcomes sharing considerable similarities with the CRISPR-Cas9 system in protoplast. Cas-SF01 also induces high efficiency mutagenesis for multiple target sites in stable transformed oilseed rape lines, generating mutants with multilocular silique and male sterile phenotypes. Furthermore, Cas-SF01-derived cytosine base editors (CBEs) were developed to produce targeted C-to-T base edits. Compared to SpCas9, Cas-SF01 has an expanded PAM range and effectively recognizes TTN PAMs, which has substantially broadened the scope of editable sites within the rapeseed genome. No mutations were identified at the putative off-target sites among the edited plants. This study developed a robust, first-of-its-kind Cas12 system in the allotetraploid Brassica napus, expanding the scope of editing and enriching genome-editing toolkits for biological research and genetic improvement.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3220-3230"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient transformation and gene editing system for Lilium species.","authors":"Yajie Zhao, Yixuan Li, Jinxin Shi, Yutong Chen, Jingwei Wei, Jinlong Li, Yiqing Wang, Dingyi Liu, Dong Jing, Shuo Long, Manman Zhang, Junwei Tang, Jian Wu","doi":"10.1111/jipb.70237","DOIUrl":"10.1111/jipb.70237","url":null,"abstract":"<p><p>A transformation system exploits lily bulb scale propagation and de novo formation of bulblets, integrating tissue culture with non-tissue culture approaches. Through optimized scale propagation and disinfection treatments, it achieves efficient stable gene overexpression and editing in lily.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3283-3285"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the power and potential of the Cas12 family members and their ancestors for crop genome editing.","authors":"Lanqin Xia, Jinsheng Lai, Jian-Kang Zhu","doi":"10.1111/jipb.70361","DOIUrl":"10.1111/jipb.70361","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3157-3159"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}