Journal of Integrative Plant Biology最新文献

{"title":"Global identification of key genes for pollen germination in rice through high-throughput screening and gene editing.","authors":"Eui-Jung Kim, Woo-Jong Hong, Yu-Jin Kim, Eun Young Kim, Sang Dae Yun, Sunok Moon, Su-Kyoung Lee, Soon Ki Park, Ki-Hong Jung","doi":"10.1111/jipb.13900","DOIUrl":"https://doi.org/10.1111/jipb.13900","url":null,"abstract":"<p><p>Successful reproduction depends on the stable germination and growth of the pollen tubes (PT). However, the molecular mechanisms involved in rice PT growth and development remain largely unknown. In a previous study, microarray transcriptome analysis identified 627 genes preferentially expressed in the tricellular and germinating pollen of rice (i.e., Oryza sativa ssp. japonica). To elucidate key genes involved in the gene transfer process facilitated by male gametophytes, we systematically screened T-DNA lines containing disrupted sequences that corresponded to these 627 genes and analyzed the genotypes of heterozygote progeny from 107 T-DNA-indexed lines covering 105 genes. We found that 42 lines exhibited a distorted segregation ratio among the wild-type (WT), heterozygote (HT), and homozygote (HM) genotypes, which deviated from the expected Mendelian ratio of 1:2:1 (WT:HT:HM). Further characterization using CRISPR/Cas9 mutants revealed that knockout mutants of certain genes that exhibited segregation distortion in the T-DNA insertion region were completely sterile. Moreover, even when T-DNA insertion lines followed Mendelian segregation patterns, sterility could be induced by simultaneously mutating functionally redundant genes, thereby overcoming genetic compensation. Interestingly, although some T-DNA insertion lines exhibited segregation ratios approximating 1:1:0, the corresponding CRISPR/Cas9 mutants produced homozygous seeds and showed partial sterility. Partial sterility suggests that despite mutant pollen grains being less competitive than WT pollen, they retain their fertilization potential under relaxed competition from WT pollen. Beyond mutant-based analysis, transcriptomic profiling of sterile mutant lines provided additional insight into the regulatory relationship between key germination regulators and the 105 target genes studied here. Overall, this study demonstrates the effectiveness of a multi-pronged strategy to accelerate the identification of defective phenotypes using mutant studies and provides valuable genetic resources for inducing novel male sterility in rice.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genomic analysis unravels the evolutionary processes leading to budding speciation.","authors":"Xiao-Ying Liu, Long Huang, Ya-Peng Yang, Yue-Yi Li, Zi-Wei Ma, Shi-Yu Wang, Lin-Feng Qiu, Qing-Song Liu, Jian-Qiang Zhang","doi":"10.1111/jipb.13905","DOIUrl":"https://doi.org/10.1111/jipb.13905","url":null,"abstract":"<p><p>Budding speciation is a process wherein a new species arises from a small, isolated population within or at the margin of an ancestral species. Well-documented cases of budding speciation are rare, and the roles of various evolutionary factors in this process remain controversial. Based on whole-genome resequencing data from 272 individuals across 27 populations, we reconstructed the evolutionary history of Rhodiola sect. Trifida and explored the relative contributions of natural selection, genetic drift, and chromosomal rearrangements as drivers of lineage divergence. We found that all samples of R. chrysanthemifolia (including R. alterna and R. sinuata) were clustered into three clades. Rhodiola liciae was sister to all other samples in the section, likely due to post-divergence gene flow and the minimal population structure of the progenitor species, while it shared the same ancestry with R. ch-I in population structure analyses. The two populations of R. sinuata were not monophyletic, instead clustering with geographically proximate populations of R. ch-III. Demographic analyses revealed that R. liciae underwent a contraction in population size following its divergence from R. ch-I approximately 0.34 million years ago (Mya), and has remained stable since around 0.1 Mya. Genomic islands and genotype-environment association analyses suggested that genetic drift and the assorting of ancestral polymorphism may have played a more significant role in the speciation of R. liciae than nature selection or chromosomal rearrangements. We propose that R. liciae diverged from R. chrysanthemifolia through budding speciation, although post-divergence gene flow has obscured its phylogenetic signal. Additionally, we identified two potential parallel budding speciation events in R. sinuata at an earlier stage than R. liciae. Our study highlights budding speciation as a prevalent yet poorly characterized mode of plant speciation, with assorting of ancestral polymorphism as a key stochastic mechanism in the process.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orchestration of leaf curvature by the SBP transcription factor SPL10-REVOLUTA module in Arabidopsis.","authors":"Pengfei Xu, Qihui Wan, Wenna Shao, You Wu, Feijie Wu, Xiaorong Li, Wenqing Ren, Yuke He, Shuxia Li, Xiang Yu","doi":"10.1111/jipb.13893","DOIUrl":"https://doi.org/10.1111/jipb.13893","url":null,"abstract":"<p><p>Leaf curvature significantly contributes to important economic traits in vegetable crops. The upward-curling leaf phenotype has been consistently observed upon overexpression of a miR156/157-resistant version of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 10 (SPL10) transcription factor (rSPL10). However, the role of SPL10 in regulating leaf curvature has not been well characterized. In this study, using DNA affinity purification sequencing followed by transient transactivation assays, we found that SPL10 can bind to the promoter and gene body of REVOLUTA (REV), augmenting its expression. The rSPL10 rev-6 double mutant plant displayed a downward-curling leaf phenotype similar to the rev-6 plant, supporting the notion that REV functions downstream of SPL10. Importantly, the SPL10 protein physically interacts with the REV protein, which attenuates the expression of REV promoted by SPL10, leading to the downregulation of REV-regulated genes involved in leaf curvature, such as HB2 and HB4. These findings suggest that the SPL10-REV module acts as a molecular rheostat to prevent excessive amplification of REV transcripts in Arabidopsis. Furthermore, overexpression of the BrpREV1 gene in Chinese cabbage caused the transformation of rosette leaves from flat to upward-curving and accelerated heading. Taken together, our findings reveal the role of SPL10-REV module in orchestrating leaf curvature, which could potentially be utilized for molecular breeding of economical traits in vegetable crops.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A SlMYB78-regulated bifunctional gene cluster for phenolamide and salicylic acid biosynthesis during tomato domestication, reducing disease resistance.","authors":"Peng Cao, Linghao Xia, Xianggui Li, Meng Deng, Zhonghui Zhang, Xiangyu Lin, Zeyong Wu, Yingchen Hao, Penghui Liu, Chao Wang, Chun Li, Jie Yang, Jun Lai, Jun Yang, Shouchuang Wang","doi":"10.1111/jipb.13899","DOIUrl":"https://doi.org/10.1111/jipb.13899","url":null,"abstract":"<p><p>Plants have evolved a sophisticated chemical defense network to counteract pathogens, with phenolamides and salicylic acid (SA) playing pivotal roles in the immune response. However, the synergistic regulatory mechanisms of their biosynthesis remain to be explored. Here, we identified a biosynthetic gene cluster on chromosome 2 (BGC2) associated with the biosynthesis of phenolamide and SA, wherein the key component SlEPS1 exhibits dual catalytic functions for the synthesis of phenolamides and SA. Overexpression of the key component SlEPS1 of BGC2 in tomato enhanced resistance to the bacterial pathogen Pst DC3000, whereas knockout plants were more susceptible. Exogenous applications of SA and phenolamides revealed that these two compounds act synergistically to enhance plant resistance. Notably, during tomato domestication, a disease-resistant allele of SlEPS1, SlEPS1^HapB, was subject to negative selection, leading to a reduction in phenolamide and SA levels and compromised disease resistance in modern varieties. Moreover, the SlMYB78 directly regulates the BGC2 gene cluster to enhance phenolamide and SA biosynthesis, modulating resistance to Pst DC3000. Our study employed multi-omics approaches to describe the synergistic regulation of phenolamide and SA biosynthesis, offering new insights into the complexity of plant immune-related metabolism.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VvHY5 and VvBEE1 antagonistically control resveratrol biosynthesis to mitigate high light-induced damage in grapevine.","authors":"Zain Ali, Yanzhao Sun, Zhaodong Ma, Yanyan Zheng, Yang Liu","doi":"10.1111/jipb.13895","DOIUrl":"https://doi.org/10.1111/jipb.13895","url":null,"abstract":"<p><p>Excessive exposure to high light can lead to photoinhibition, which impairs photosynthetic efficiency and causes oxidative damage in plants, such as sunburn in grapevines. This study investigates the role of resveratrol (Res), a stilbenoid with antioxidant properties, in protecting plants from high light damage. We found that exposure to high light increased reactive oxygen species (ROS) accumulation and induced photoinhibition in grapevine leaves. In response, Res biosynthesis was upregulated, along with an increase in stilbene synthase (VvSTS) expression. Application of exogenous Res alleviated ROS accumulation and improved photosynthetic efficiency. Further analysis revealed that the VvHY5-VvBEE1 regulatory module plays a pivotal role in regulating VvSTS expression under high light conditions. Specifically, VvHY5 activated VvSTS expression, while VvBEE1 repressed it. Transgenic analysis showed that overexpression of VvHY5 enhanced Res production and photoprotection, whereas overexpression of VvBEE1 reduced Res levels and exacerbated light-induced damage. VvHY5 and VvBEE1 competed for binding to the VvSTS promoter, with brassinosteroids (BRs) modulating their interaction. Our findings reveal the interplay between light signaling and brassinosteroid pathways in regulating Res biosynthesis, providing insights for protecting grapevines from sunburn.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13688","DOIUrl":"https://doi.org/10.1111/jipb.13688","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 3","pages":"423-424"},"PeriodicalIF":9.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13688","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FaNAC047-FaNAC058 module coordinately promotes chlorophyll degradation and reactive oxygen species production during heat-induced leaf senescence in tall fescue.","authors":"Liwen Cao, Yao Chen, Kai Xiao, Liang Chen","doi":"10.1111/jipb.13897","DOIUrl":"https://doi.org/10.1111/jipb.13897","url":null,"abstract":"<p><p>Leaf senescence can be triggered by various abiotic stresses. Among these, heat stress emerges as a pivotal environmental factor, particularly in light of the predicted rise in global temperatures. However, the molecular mechanism underlying heat-induced leaf senescence remains largely unexplored. As a cool-season grass species, tall fescue (Festuca arundinacea) is an ideal and imperative material for investigating heat-induced leaf senescence because heat stress easily triggers leaf senescence to influence its forage yield and turf quality. Here, we investigated the role of FaNAC047 in heat-induced leaf senescence. Overexpression of FaNAC047 promoted heat-induced leaf senescence in transgenic tall fescue that was evidenced by a more seriously destructive photosystem and higher accumulation of reactive oxygen species (ROS), whereas knockdown of FaNAC047 delayed leaf senescence. Further protein-DNA interaction assays indicated that FaNAC047 directly activated the transcriptions of NON-YELLOW COLORING 1 (FaNYC1), NYC1-like (FaNOL), and STAY-GREEN (FaSGR) but directly inhibited Catalases 2 (FaCAT2) expression, thereby promoting chlorophyll degradation and ROS accumulation. Subsequently, protein-protein interaction assays revealed that FaNAC047 physically interacted with FaNAC058 to enhance its regulatory effect on FaNYC1, FaNOL, FaSGR, and FaCAT2. Additionally, FaNAC047 could transcriptionally activate FaNAC058 expression to form a regulatory cascade, driving senescence progression. Consistently, the knockdown of FaNAC058 significantly delayed heat-induced leaf senescence. Collectively, our results reveal that FaNAC047-FaNAC058 module coordinately mediates chlorophyll degradation and ROS production to positively regulate heat-induced leaf senescence. The findings illustrate the molecular network of heat-induced leaf senescence for breeding heat-resistant plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13689","DOIUrl":"https://doi.org/10.1111/jipb.13689","url":null,"abstract":"<p>This special issue features invited expert reviews that focus on the latest progress in the fields of plant growth and development, stress resistance, functional omics, and molecular breeding. The cover centers around a vibrant “Tree of Life”, its lush canopy adorned with key research species such as apples, soybeans, tomatoes, tobacco, Arabidopsis, wheat, corn, cotton, rice, and alfalfa. Surrounding the tree are depictions of plant viruses, hormones, and chemical elements, vividly highlighting current research hotspots. Together, they present a magnificent overview of the fl ourishing field of plant biological science.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 3","pages":"C1"},"PeriodicalIF":9.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13689","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KatB, a bacterial extracellular vesicles (EVs)-secreted catalase, detoxifies reactive oxygen species (ROS) and promotes pathogen proliferation in plants.","authors":"Jiliang Deng, Wei Li, Zhangying Wang, Jiayue Zeng, Qiang Cai","doi":"10.1111/jipb.13894","DOIUrl":"https://doi.org/10.1111/jipb.13894","url":null,"abstract":"<p><p>Gram-negative bacteria are known to release extracellular vesicles (EVs) into their surrounding environment. However, the biological functions of the proteins contained within these vesicles remain largely unknown. Here, we used tandem mass tag (TMT) proteomic analysis to characterize protein cargoes within EVs of the phytopathogen Pseudomonas syringae pv. tomato DC3000 (Pto DC3000). Our investigation revealed that one catalase, KatB, is enriched in bacterial EVs. This enzyme confers EVs with the capacity to detoxify both exogenous and plant-produced H₂O₂, thereby contributing to the pathogen's proliferation within the plants. Interestingly, reactive oxygen species (ROS) stress stimulates bacterial EV secretion and enhances the package of KatB into these vesicles. This regulatory process depends on a periplasmic ankyrin-like protein, AnkB. Both AnkB and KatB are encoded within a small operon, and their mutant strains exhibit impaired growth in plant hosts. Furthermore, the treatment of EVs pelleted from bacterial culture supernatants activates the immune responses of plants, and the absence of KatB in EVs further enhances this protective activity. Collectively, our findings indicate that bacteria secreted KatB via EVs to interact with and reduce the host's oxidative environment, thereby promoting their proliferation within plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of elite rice with broad-spectrum resistance through pyramiding of key resistance gene and simultaneously editing multiple susceptibility genes.","authors":"Hui Tao, Ning Xiao, Ruyi Wang, Feng He, Yue Cai, Su Jiang, Min Wang, Dan Wang, Huamin Chen, Xiaoman You, Aihong Li, Guo-Liang Wang, Yuese Ning","doi":"10.1111/jipb.13901","DOIUrl":"https://doi.org/10.1111/jipb.13901","url":null,"abstract":"<p><p>Knocking out three susceptibility genes (Pi21, Bsr-d1, and Xa5) in a rice breeding line that contains the resistance Piz-t produced enhanced broad-spectrum resistance against the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae without obvious growth penalty.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}