Yuan Deng, Chenyue Yang, Yaru Wu, Yongke Zhou, Ying Chen, Cun Wang, Zhenqian Zhang, Chuanfeng Ju
{"title":"CPK10 phosphorylates zinc-sensing transcription factors bZIP19/23 to establish time-delayed negative feedback in plant zinc homeostasis.","authors":"Yuan Deng, Chenyue Yang, Yaru Wu, Yongke Zhou, Ying Chen, Cun Wang, Zhenqian Zhang, Chuanfeng Ju","doi":"10.1111/jipb.70423","DOIUrl":"https://doi.org/10.1111/jipb.70423","url":null,"abstract":"<p><p>Zinc (Zn) homeostasis is essential for plant growth; yet, how zinc-deficiency-triggered calcium (Ca) signals are decoded into homeostatic adjustments remains elusive. Here, we identify the calcium-dependent protein kinase 10 (CPK10) as a key decoder of zinc-deficiency-triggered Ca<sup>2+</sup> signals. Under zinc-deficient conditions, both the protein level and the kinase activity of CPK10 increase. Subsequently, CPK10 interacts with and phosphorylates the zinc-sensing transcription factors bZIP19 and bZIP23, thereby repressing their DNA-binding and transcriptional activities. Zrt/Irt-like protein (ZIP) family members are the primary transporters responsible for zinc uptake from the soil, and CPK10-mediated phosphorylation of bZIP19 and bZIP23 reduces the expression of ZIP1, ZIP4, and defensin-like (DEFL) genes such as DEFL208. CPK10 activation is time-delayed, peaking at 72 h after zinc-deficiency treatment, providing a braking mechanism that prevents prolonged high-rate ion flux driven by sustained high-level expression of zinc transporters. The CPK10-targeted phosphorylation site Ser241 of bZIP19 is conserved in dicots but absent in monocots, suggesting evolutionary divergence in this zinc homeostasis-regulatory module. Our work reveals a Ca<sup>2+</sup>-CPK10-bZIP19/23 module that mediates spatiotemporal control of zinc homeostasis, highlighting a key regulatory layer in plant nutrient signaling.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinman Luo, Lei Yan, Jingying Li, Chen Zhang, Shaoya Li, Yubing He, Yan Guo, Lanqin Xia
{"title":"Creation of fragrant wheat by targeted mutagenesis of either BADH1 or both BADH1 and BADH2 using CRISPR/Cas9.","authors":"Jinman Luo, Lei Yan, Jingying Li, Chen Zhang, Shaoya Li, Yubing He, Yan Guo, Lanqin Xia","doi":"10.1111/jipb.70422","DOIUrl":"https://doi.org/10.1111/jipb.70422","url":null,"abstract":"<p><p>Editing TaBADH1 alone or TaBADH1 and TaBADH2 via CRISPR/Cas9 produced fragrant wheat with up to 11.24-fold higher 2-acetyl-1-pyrroline than the wild type without yield penalty, revealing asymmetric functional redundancy between these two genes and providing elite germplasm for wheat quality improvement.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Two linked nitrate reductase genes CR8.1 and CR8.2 positively coregulate yield and NUE in rice.","authors":"Linlin Hou, Dongdong Chen, Guang Chen, Jiajia Liu, Hongge Qian, Lianguang Shang, Xiangjian Pan, Shenghang Jiang, Guangheng Zhang, Longbiao Guo, Guojun Dong, Li Zhu, Jiang Hu, Qing Li, Lan Shen, Qiang Zhang, Qian Qian, Guoyou Ye, Zhenyu Gao","doi":"10.1111/jipb.70420","DOIUrl":"https://doi.org/10.1111/jipb.70420","url":null,"abstract":"<p><p>The qCR8 locus contains two linked nitrate reductase genes, CR8.1 and CR8.2. Genetic and biochemical evidence shows that their additive activity promotes nitrate uptake and transport, grain yield and nitrogen use efficiency, and that alleles from the cultivar 9311 are favorable for rice breeding.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genomic dissection of divergent selection reveals a roadmap for reciprocal improvement of Chinese and American soybean.","authors":"Chengzhi Jiao, Delin Li, Hao Zhang, Zhongren Zhang, Xueqing Wang, Long Yan, Tianqing Zheng, Chaosen Zhao, Hongmei Qiu, Guangnan Xing, Guoyu Hu, Like Liu, Qiang Wang, Wen Huang, Xing Wang, Yumin Wang, Long Miao, YanFei Li, Xin Jing, Xiaojian Fang, Yu Tian, Ying-Hui Li, Xiaobo Wang, Li-Juan Qiu","doi":"10.1111/jipb.70421","DOIUrl":"https://doi.org/10.1111/jipb.70421","url":null,"abstract":"<p><p>Soybean (Glycine max [L.] Merr.) was domesticated in China over five millennia ago; yet, its global expansion has resulted in two distinct, independently evolved breeding pools in China and the Americas. While regional breeding has optimized specific traits, a comprehensive roadmap for the reciprocal exchange of these \"breeding-specific\" beneficial alleles is lacking. Here, we performed a large-scale genomic dissection of 2,377 soybean accessions, integrating 8.4 million high-quality SNPs with multi-environment phenotyping across 11 locations. Through genome-wide association studies (GWAS), we identified 261 significant loci (223 novel) governing six key agronomic traits, revealing that Chinese and American germplasms have accumulated distinct sets of beneficial alleles of seed size/protein in China and oil/yield in the Americas. To harness this untapped potential, we developed the Soybean Targeted Improvement Navigation System (STINS), a framework integrating population genomics, GWAS, and genomic selection (GS). We demonstrate the potential of targeted beneficial allele introgression between populations via gene modification for rapid trait improvement. Proof-of-concept functional evaluation through CRISPR/Cas9-mediated knockout of GmST05 in an American cultivar and overexpression of a resistant GmSNAP18 haplotype in a Chinese cultivar provided experimental support for STINS-predicted targets for protein content and SCN resistance improvement. Our study provides a navigable genomic resource for \"knowledge-driven design breeding\", offering a blueprint for the global exchange of elite alleles to ensure future food security.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yixue Bai, Dongqian Shan, Chanyu Wang, Zehui Hu, Tianci Yan, Tong Zhang, Xin Liu, Handong Song, Zhaoyang Zhou, Jun Liu, Yan Guo, Wei Li, Xiaodong Zheng, Jin Kong
{"title":"The BIK1-WRKY9 module activates jasmonate signaling to confer apple resistance to Alternaria blotch.","authors":"Yixue Bai, Dongqian Shan, Chanyu Wang, Zehui Hu, Tianci Yan, Tong Zhang, Xin Liu, Handong Song, Zhaoyang Zhou, Jun Liu, Yan Guo, Wei Li, Xiaodong Zheng, Jin Kong","doi":"10.1111/jipb.70414","DOIUrl":"https://doi.org/10.1111/jipb.70414","url":null,"abstract":"<p><p>Apple Alternaria blotch, caused by Alternaria alternata f. sp. mali (A. alternata), is a devastating fungal disease threatening global apple production. However, the early signaling pathway underlying apple resistance to A. alternata remains unclear. In this study, through transcriptomic analysis, we identified that MdWRKY9 is a key positive regulator of apple resistance to A. alternata, whose expression is induced in the resistant \"Hanfu\" while being reduced in the susceptible \"Gala\" following A. alternata infection. Stable overexpression of MdWRKY9 enhanced apple resistance to A. alternata, whereas RNA interference (RNAi)-mediated knockdown of MdWRKY9 increased susceptibility. We further demonstrated that MdWRKY9 directly represses the expression of MdJAZ8, a key repressor of jasmonate (JA) signaling. Accordingly, stable overexpression of MdJAZ8 in apple calli reduced resistance to A. alternata and suppressed JA signaling. Consistently, transient overexpression of MdJAZ8 in apple leaves decreased resistance to A. alternata, whereas transient silencing of MdJAZ8 via RNAi enhanced disease resistance. Furthermore, we identified that the receptor-like cytoplasmic kinase MdBIK1 (BOTRYTIS-INDUCED KINASE1, a core component of the PTI pathway) interacts with and phosphorylates MdWRKY9 at Thr-2. The phosphorylation of MdWRKY9 enhances resistance to A. alternata by further suppressing MdJAZ8 and activating JA signaling. Together, our findings define a complete PTI-associated signaling module in which the MdBIK1-MdWRKY9 complex enhances JA-mediated immunity against A. alternata by directly repressing MdJAZ8. This study refines the regulatory mechanism by which the BIK1 family kinases modulate JA signaling, providing new insights into the plant immune signaling network.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feng Cui, Jingxue Li, Hongyu Bi, Sichen Zhao, Xiaoqi Yuan, Boya Zhao, Liang Xu, Yinbo Ma, Xiaoli Zhang, Sen Chen, Yuting Zhang, Yan Wang, Liwang Liu
{"title":"Single-nucleus RNA sequencing reveals a key regulator RsHB8 affecting vascular cambium activity and xylem differentiation during radish taproot thickening.","authors":"Feng Cui, Jingxue Li, Hongyu Bi, Sichen Zhao, Xiaoqi Yuan, Boya Zhao, Liang Xu, Yinbo Ma, Xiaoli Zhang, Sen Chen, Yuting Zhang, Yan Wang, Liwang Liu","doi":"10.1111/jipb.70417","DOIUrl":"https://doi.org/10.1111/jipb.70417","url":null,"abstract":"<p><p>Radish taproot thickening is primarily driven by vascular cambium (VC) activity. However, the transcriptional regulatory mechanisms underlying taproot thickening at a single-cell resolution remain unclear. In this study, single-nucleus isolation coupled with fluorescence-activated cell sorting was used to construct a cell atlas of radish taproots using microdroplet-based single-nucleus RNA sequencing. Through canonical marker gene profiling and validation using laser capture microdissection transcriptome data, 12 cell types were identified, and the first single-nucleus transcriptomic atlas was constructed for radish taproots. VC cells exhibited heterogeneous transcriptional states with varying levels of expression of cell cycle-associated genes. Pseudotime analysis inferred a transcriptional trajectory from VC cells toward xylem parenchyma cells in radish taproots. Integrating pseudotime analysis with predicted protein-protein interaction networks highlighted HOMEOBOX 8 (RsHB8) as a candidate regulator of the inferred VC-to-xylem parenchyma (XP) trajectory. RsHB8m overexpression increased taproot width and promoted xylem differentiation, whereas RsHB8-silenced plants exhibited a reduced taproot width and narrower xylem region. Xyloglucan endotransglucosylase/hydrolase 22 (RsXTH22) was identified as a target gene of RsHB8. LOB domain-containing protein 21 (RsLBD21) interacted with RsHB8, inhibited its transcriptional activity, and attenuated the RsHB8-mediated transcriptional activation of RsXTH22. Silencing RsLBD21 significantly increased taproot and xylem width, whereas silencing RsXTH22 resulted in the opposite phenotypes. Together, these findings establish a single-nucleus transcriptomic atlas of radish taproot development and provide insights into the transcriptional regulation of VC differentiation during taproot thickening.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"S‑glutathionylation of MaWRKY44 orchestrates a MaZFP139-MaWRKY44-MaGSTU07 cascade to enhance banana cold tolerance.","authors":"Shuqin Lin, Wen Song, Shaoting Liao, Haike Tan, Jianji Qi, Danling Cai, Wei Wei, Wei Shan, Jianfei Kuang, Jianye Chen, Wangjin Lu","doi":"10.1111/jipb.70419","DOIUrl":"https://doi.org/10.1111/jipb.70419","url":null,"abstract":"<p><p>Chilling injury caused by low temperatures severely reduces the postharvest quality and marketability of banana fruit. Although glutathione S‑transferase (GST)-mediated reactive oxygen species (ROS) scavenging constitutes a core antioxidant defense against cold oxidative stress, the transcriptional and post‑translational mechanisms governing this process remain largely elusive. Here, we discovered that S‑glutathionylation of the WRKY transcription factor MaWRKY44 functions as a previously unknown redox switch that orchestrates a hierarchical transcriptional cascade to enhance cold tolerance. LC‑MS/MS identified Cys45 and Cys199 as conserved S‑glutathionylation sites of MaWRKY44. Biochemical and molecular analyses revealed a biphasic redox switch: Mild ROS triggers reversible glutathionylation to stabilize MaWRKY44 and enhance its DNA‑binding capacity, whereas sustained oxidative stress drives irreversible cysteine over‑oxidation that abolishes its transcriptional function. Mechanistically, we identified a C2H2 zinc finger transcription factor, MaZFP139, as a direct upstream activator of MaWRKY44, which in turn specifically binds to the W‑box motifs of the MaGSTU07 promoter to drive its transcription. Transient gain‑ and loss‑of‑function assays in banana, together with stable heterologous transformation in tomato, demonstrated that the MaZFP139-MaWRKY44-MaGSTU07 cascade positively mitigates cold‑triggered ROS accumulation and peel browning. Collectively, our findings uncover a multilayer transcriptional‑redox module governing fruit cold tolerance, and provide potential molecular targets for improving postharvest cold storage performance in tropical fruits.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TaANT11-4A enhances grain yield by orchestrating photosynthesis and carbohydrate metabolism through direct transcriptional activation.","authors":"Pengpeng Zhang, Jingjing Wen, Shan Gao, Jiajun Ma, Jiahui Jin, Xiaofei Ma, Wen-Ting Liu, Juan Li, De-Jun Han, Yue-Qiang Zhang, Kun-Ming Chen","doi":"10.1111/jipb.70416","DOIUrl":"https://doi.org/10.1111/jipb.70416","url":null,"abstract":"<p><p>The AINTEGUMENTA (ANT) transcription factor family plays important roles in floral organogenesis and plant development; however, the mechanisms by which ANT proteins regulate yield remain unclear. Here, we conducted a genome-wide search and identified 64 ANT genes in the wheat genome. Association analysis of yield-related traits in 403 wheat accessions revealed that ANT family genes were significantly associated with grain size and weight. Among these genes, TaANT11-4A showed a strong association with yield-related traits. Three single-nucleotide polymorphisms (SNPs) near TaANT11-4A modulated its expression and defined two haplotypes. TaANT11-4A<sup>Hap2</sup> was identified as an elite allele associated with increased grain size. Overexpression of TaANT11-4A significantly increased grain size and overall plant biomass, largely owing to higher photosynthetic rates and increased activities of key enzymes involved in photosynthesis and carbohydrate metabolism in the transgenic plants. Further analyses demonstrated that TaANT11-4A directly binds to and activates TaEGY2, TaAAE1, and TaAAE2, which are involved in photosynthesis and carbohydrate metabolism. Consistent with these findings, knockout of TaEGY2 reduced grain size and yield, whereas overexpression of TaEGY2 or TaAAE2 increased these traits relative to the wild type (WT). Collectively, our results demonstrate that TaANT family proteins, particularly TaANT11-4A, are key regulators of wheat grain size and yield through the direct transcriptional activation of genes that coordinate photosynthesis and carbohydrate metabolism.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sunan Gao, Long Cui, Licheng Xiao, Rong Huang, Jing Wei, Chunrui Chen, Wenran Su, Jiajun Ran, Lei Song, Jingyi Li, Taotao Wang, Zonglie Hong, Zhibiao Ye, Jie Ye, Junhong Zhang
{"title":"SlBPC1 regulates fruit elongation by directly repressing SlOFP2/SlOFP21 and SUN expression in tomato.","authors":"Sunan Gao, Long Cui, Licheng Xiao, Rong Huang, Jing Wei, Chunrui Chen, Wenran Su, Jiajun Ran, Lei Song, Jingyi Li, Taotao Wang, Zonglie Hong, Zhibiao Ye, Jie Ye, Junhong Zhang","doi":"10.1111/jipb.70409","DOIUrl":"https://doi.org/10.1111/jipb.70409","url":null,"abstract":"<p><p>Fruit shape is an important agronomical trait that influences consumer preference. Despite the dramatic variations in fruit shape among tomato germplasms, the underlying genes and regulatory mechanisms remain poorly understood. Here, we found that knockout of the transcription factor SlBPC1 leads to elongated fruits, resulting from altered cell division patterns during ovary development. Further studies revealed that SlBPC1 regulates fruit shape by repressing its target genes SlOFP21 and SUN. Overexpression of SlOFP21 resulted in the formation of elongated fruits with an increased fruit shape index. Moreover, SlBPC1 also represses the expression of SlOFP2, a homolog of SlOFP21. Consistently, overexpression of SlOFP2 also led to elongated fruits in tomato. SlOFP2 and SlOFP21 were found to interact with fruit elongation activator SlTRM5 and repressors SlTRM17/20a and SlTRM19. Together, our results revealed an SlBPC1-SlOFP2/SlOFP21/SUN regulatory module that provides new insights and molecular tools for the genetic improvement of fruit shape traits in tomato breeding programs.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
