Journal of Integrative Plant Biology最新文献_第10页

Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica). 磁性纳米粒子介导枇杷遗传转化与基因编辑系统。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-05-28 DOI: 10.1111/jipb.70307

Xiaodong Suo, Haiyan Wang, Tingting Meng, Luwei Wang, Shuai Wang, Xun Xu, Shi Wang, Mubasshir Hussain, Danlong Jing, Jiangbo Dang, Di Wu, Qigao Guo

{"title":"Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica).","authors":"Xiaodong Suo, Haiyan Wang, Tingting Meng, Luwei Wang, Shuai Wang, Xun Xu, Shi Wang, Mubasshir Hussain, Danlong Jing, Jiangbo Dang, Di Wu, Qigao Guo","doi":"10.1111/jipb.70307","DOIUrl":"10.1111/jipb.70307","url":null,"abstract":"Loquat (Eriobotrya japonica Lindl.) is a valuable subtropical fruit tree whose genetic improvement has been significantly constrained by the absence of an efficient genetic transformation system. Although Agrobacterium-mediated transformation is the most widely used method, it proves ineffective in loquat due to the species' recalcitrance to in vitro regeneration. Pollen-based transformation offers a promising alternative by bypassing the need for tissue culture. However, the pollen wall poses a major physical barrier to the uptake of exogenous DNA. In this study, we investigated magnetic nanoparticle (MNP)-mediated transformation as a novel strategy for loquat. We confirmed that loquat pollen contains tricolporate apertures with diameters ranging from 3.0 to 5.0 μm, which are structurally suitable for the entry of MNPs-DNA. Based on this finding, we developed and optimized a transformation protocol using polyethyleneimine-coated Fe3O4 nanoparticles to deliver genetic material into loquat pollen grains. Using this approach, we successfully generated stable transgenic loquat lines, including both overexpression and gene-edited mutants. To our knowledge, this is the first report of successful MNP-mediated pollen transformation in a woody plant species. This work establishes a robust and efficient genetic transformation platform for loquat, providing a valuable tool for functional genomics and molecular breeding, as well as a potentially applicable strategy for other recalcitrant woody plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3272-3282"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148051271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intron editing of RISBZ1 confers thermotolerance for grain filling in rice. RISBZ1内含子编辑可提高水稻籽粒灌浆的耐热性。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-22 DOI: 10.1111/jipb.70274

Linhan Li, Lin Zhou, Hong Jiang, Long Huang, Kangwei Ji, Yijie Li, Zhonghao Yao, Ziang Li, Yongmei Bao, Ji Huang, Mengcen Wang, Wei Wang, Hongsheng Zhang, Sunlu Chen

引用次数: 0

A strategy for decreasing prolamin content in rice endosperm through gene editing. 通过基因编辑降低水稻胚乳中蛋白含量的策略。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-08-11 DOI: 10.1111/jipb.70371

Ziyan Shen, Youguang Li, Yuyan Huang, Tao Wang, Shijie Jian, Zihao Guan, Yuntong Feng, Xiangyuan Dang, Xinlan Shan, Shengyuan Sun, Min Guo, Zhi Hu, Yihao Yang, Changjie Yan

引用次数: 0

Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants. 增强的外切酶- cas9系统在植物中促进多核苷酸缺失，具有更高的效率和更广泛的靶向范围。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-01-21 DOI: 10.1111/jipb.70155

Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang

{"title":"Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants.","authors":"Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang","doi":"10.1111/jipb.70155","DOIUrl":"10.1111/jipb.70155","url":null,"abstract":"CRISPR-Cas9 is a widely used platform for plant genome editing, but its outcomes are typically dominated by small insertions and deletions (indels). Such limited mutation profiles restrict its utility in functional studies of non-coding RNAs and regulatory elements, such as microRNAs (miRNAs), untranslated regions (UTRs), and promoter sequences, where larger sequence disruptions are often required. Here, we developed enhanced exonuclease-Cas9 platforms, termed multiple nucleotide deletion Cas9 (MND-Cas9) systems, for efficient generation of large deletions in rice. By screening four exonucleases (RecJ, T5, TREX2, and SbcB), we established MND-Cas9v1 systems based on TREX2 or SbcB that produced substantially larger deletions without reducing editing efficiency. Further optimization with an inserted DNA-binding domain (DBD) between Cas9 and exonuclease yielded MND-Cas9v2, which simultaneously enhanced efficiency and deletion size. To expand PAM compatibility, we introduced PAM-relaxed Cas9-NG and SpG variants, generating MND-Cas9-NG/SpGv2 systems with broader targeting scope and superior performance compared to their parental nucleases. Finally, we demonstrated the utility of these systems in two applications: MND-Cas9v2 efficiently knocked out the miRNA gene OsMIR530, producing larger seeds, and generated extended deletions in the 3'UTR of OsGhd2, which upregulated its expression and increased grain size. These results demonstrate that MND-Cas9 systems enable high-efficiency generation of extended deletions and facilitate functional analyses of non-coding RNAs and regulatory sequences. Overall, this work establishes a versatile and expandable exonuclease-Cas9 platform that substantially broadens the mutational spectrum and application potential of CRISPR-Cas9 for plant genome engineering.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3316-3328"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eukaryotic chaperonin coordinates root meristem activity by regulating SEC31B-dependent COPII vesicle trafficking of PIN auxin efflux carrier in Arabidopsis. 真核伴侣蛋白通过调节sec31b依赖性COPII囊泡运输PIN生长素外排载体来协调根分生组织活性。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-05-04 DOI: 10.1111/jipb.70277

Mengjuan Tong, Yinghui Zhang, Aiwei Zhang, Jingsheng Zhang, Yiqiang Li, Nan Chen, Xiaoyu Liu, Yirui Zhu, Juntao Wang, Shuailei Wang, Zhuoxuan Li, Yi Xu, Sitong Liu, Yi Guo, Rui Li

{"title":"Eukaryotic chaperonin coordinates root meristem activity by regulating SEC31B-dependent COPII vesicle trafficking of PIN auxin efflux carrier in Arabidopsis.","authors":"Mengjuan Tong, Yinghui Zhang, Aiwei Zhang, Jingsheng Zhang, Yiqiang Li, Nan Chen, Xiaoyu Liu, Yirui Zhu, Juntao Wang, Shuailei Wang, Zhuoxuan Li, Yi Xu, Sitong Liu, Yi Guo, Rui Li","doi":"10.1111/jipb.70277","DOIUrl":"10.1111/jipb.70277","url":null,"abstract":"The eukaryotic chaperonin containing T-complex polypeptide-1 (CCT/TRiC) complex, composed of eight distinct subunits (CCT1-CCT8), is essential for cytosolic protein folding; however, its function in plants remains largely unexplored. Moreover, a direct link between CCT and coat protein complex II (COPII) vesicle trafficking-a key step in the early secretory pathway-has not been established in any eukaryotic system. Here, leveraging plant genetics, we investigated the functional relationship between CCT8 and COPII-mediated trafficking in the Arabidopsis root apex. The point mutant cct8-1 exhibited a short-root phenotype resulting from impaired cell division in the root meristem, which was accompanied by disrupted auxin homeostasis. This defect stemmed from a marked reduction in the abundance of multiple PIN-FORMED (PIN) auxin efflux carriers at the plasma membrane, without affecting their polar localization. Mechanistically, CCT8 directly interacted with SEC31B, a core component of the COPII coat. Accordingly, the sec31b-3 mutant phenocopied cct8-1 in root growth, auxin response, and PIN accumulation defects. The CCT8 mutation reduced SEC31B abundance at both the transcriptional and protein levels and compromised ER-to-Golgi transport, thereby diminishing PIN delivery to the plasma membrane. Importantly, overexpression of SEC31B partially rescued the root growth defects and restored PIN2 levels in cct8-1. Together, our findings uncover a previously unrecognized chaperonin-trafficking module in which CCT8 regulates SEC31B to modulate COPII-mediated delivery of PIN proteins, thus linking chaperonin function to auxin-dependent root development.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3409-3425"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana. 拟南芥SIB1-SEC23A通过内质网到叶绿体的再定位介导免疫。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-12 DOI: 10.1111/jipb.70251

Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia

{"title":"SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana.","authors":"Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia","doi":"10.1111/jipb.70251","DOIUrl":"10.1111/jipb.70251","url":null,"abstract":"Inter-organellar communication has emerged as a critical factor in maintaining cellular homeostasis under stress conditions. Chloroplasts function not only as central organelles for energy production but are also increasingly recognized as stress sensors and signal integrators. SIGMA FACTOR-BINDING PROTEIN 1 (SIB1), encoded by a nuclear gene, has been identified as a positive regulator of plant immunity, with localization in both chloroplasts and the nucleus. In this study, we identified Arabidopsis SEC23A, a COPII complex component known to mediate membrane trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus, as a novel interactor of SIB1. Our findings reveal that under normal conditions, both SIB1 and SEC23A localize to the ER, while SIB1 is also localized in the nucleus. Upon ER stress and/or treatment with the immunity inducer salicylate, both proteins are relocated from the ER to the chloroplasts. Notably, SEC23A, similar to SIB1, also functions as a positive regulator of disease resistance. In response to pathogen infection, SIB1 and SEC23A downregulate expression of chloroplast- and nucleus-encoded genes associated with photosynthesis while enhancing expression of defense-related genes. Together, our findings reveal a previously uncharacterized pathway of ER-chloroplast communication mediated by SIB1 and SEC23A during plant stress responses and immunity, providing novel insights into the intricate regulatory networks that govern inter-organellar communication under stress in plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3458-3472"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of low-prolamin rice germplasm via CRISPR/Cas9 editing to improve eating and cooking quality. 利用CRISPR/Cas9编辑技术开发低蛋白水稻种质，提高食用和烹饪品质。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-08-20 DOI: 10.1111/jipb.70377

Zhihui Chen, Yu Chen, Gexing Wan, Hui Dong, Tian Pan, Wenkun Yang, Yiyang Zhou, Bingke Luo, Yun Zhu, Xiaohang Han, Lianji Lu, Xin Wang, Cailin Lei, Zhichao Zhao, Jie Wang, Yulong Ren, Jianmin Wan

引用次数: 0

Development of a highly efficient prime editing platform for cucurbits enables breeding of multi-disease-resistant cucumber. 开发一种高效的葫芦素编辑平台，使多抗病黄瓜育种成为可能。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-08-19 DOI: 10.1111/jipb.70375

Junya Wang, Ling Xiao, Tongxu Xin, Juan Li, Xueyong Yang

{"title":"Development of a highly efficient prime editing platform for cucurbits enables breeding of multi-disease-resistant cucumber.","authors":"Junya Wang, Ling Xiao, Tongxu Xin, Juan Li, Xueyong Yang","doi":"10.1111/jipb.70375","DOIUrl":"10.1111/jipb.70375","url":null,"abstract":"The prime editing (PE) system is a precise genome editing technology that works efficiently in monocots; however, its application is limited by low editing efficiency in dicots, particularly Cucurbitaceae and Solanaceae plants. Here, we first significantly improved the transformation efficiency by introducing spectinomycin in cucurbits, then used the tomato elongation factor 1-alpha (SlEF1α) promoter to enhance PE protein expression, and incorporated the Csy4 ribonuclease to process pegRNAs, collectively addressing multiple constraints limiting PE efficiency in cucurbits. The optimized PE systems, particularly Csy4-PE6d, achieved an average desired editing frequency of 80.83% at targeted loci in cucumber via stable genetic transformation, with frequencies reaching up to 100% at certain sites. Moreover, Csy4-PE6d generated homozygous edits in 36.43% of transgenic lines and demonstrated robust editing activity in melon, pumpkin, and potato. Using the Csy4-PE6d tool, we generated heritable edited cucumber lines with dual resistance to bacterial angular leaf spot and downy mildew by targeting the CsSGR gene. Collectively, this optimized system substantially enhances PE efficiency in Cucurbit crops, providing an effective solution to common challenges such as low editing efficiency and limited heritability in these species.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3340-3351"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized TadA-derived base editors efficiently manipulate mRNA splicing by A-to-G and C-to-K editing in potato. 优化的tada衍生碱基编辑器通过A-to-G和C-to-K编辑有效地操纵了马铃薯mRNA剪接。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-20 DOI: 10.1111/jipb.70263

Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang

{"title":"Optimized TadA-derived base editors efficiently manipulate mRNA splicing by A-to-G and C-to-K editing in potato.","authors":"Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang","doi":"10.1111/jipb.70263","DOIUrl":"10.1111/jipb.70263","url":null,"abstract":"Pre-messenger RNA (pre-mRNA) splicing is a critical mechanism for post-transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate development as well as responses to biotic and abiotic stresses. However, modulating the generation of specific splicing isoforms for functional characterization remains challenging, particularly in the non-model crop potato. Here, we show that two optimized TadA-derived base editors efficiently induce diverse mRNA splice variants by targeting specific splice sites. By evaluating multiple adenosine deaminases and performing multi-dimensional optimization, we developed an efficient adenine base editor RTF-ABE8e for potato. RTF-ABE8e achieved 100% editing efficiency at two StDL1 target sites in stable transgenic potato, with homozygous editing frequencies as high as 93.3% and 91.1%, respectively. We also developed RTF-TadDE, a dual-base editor based on a TadA-derived dual deaminase, for A-to-G and C-to-K (K = T/G) mutations in potato with an overall editing efficiency comparable to that of RTF-ABE8e. By targeting different splice sites with these base editors, we obtained diverse splicing isoforms carrying premature termination codons (PTCs) at StDL1 and StPDS and robust mutant phenotypes. These base editors enable efficient and precise editing of splice sites to trigger missplicing, making them powerful tools for manipulating splicing in plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3329-3339"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering herbicide-resistant sorghum with CRISPR/Cas9-mediated adenine base editing. 用CRISPR/ cas9介导的腺嘌呤碱基编辑技术改造抗除草剂高粱

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-05-18 DOI: 10.1111/jipb.70298

Jianshuang Zhou, Ruirui Li, Zhi Wang, Shaoxiong Liu, Lingyue Shi, Xiao Fu, Fei Li, Ji Zhang, Guiying Li, Jinjie Zhu, Qian Qian, Baoqing Dun

引用次数: 0