Journal of Integrative Plant Biology最新文献

{"title":"Sulfur metabolism under stress: Oxidized glutathione inhibits methionine biosynthesis by destabilizing the enzyme cystathionine γ-synthase","authors":"Yael Hacham,&nbsp;Alex Kaplan,&nbsp;Elad Cohen,&nbsp;Maayan Gal,&nbsp;Rachel Amir","doi":"10.1111/jipb.13799","DOIUrl":"10.1111/jipb.13799","url":null,"abstract":"<div>\u0000 \u0000 <p>Cysteine is the precursor for the biosynthesis of glutathione, a key stress-protective metabolite, and methionine, which is imperative for cell growth and protein synthesis. The exact mechanism that governs the routing of cysteine toward glutathione or methionine during stresses remains unclear. Our study reveals that under oxidative stress, methionine and glutathione compete for cysteine and that the increased oxidized glutathione (GSSG) levels under stress hinder methionine biosynthesis. Moreover, we find that inhibition occurs as GSSG binds to and accelerates the degradation of cystathionine γ-synthase, a key enzyme in the methionine synthesis pathway. Consequently, this leads to a reduction in the flux toward methionine-derived metabolites and redirects cysteine utilization toward glutathione, thereby enhancing plant protection. Our study suggests a novel regulatory feedback loop involving glutathione, methionine, and cysteine, shedding light on the plant stress response and the adaptive rerouting of cysteine. These findings offer new insights into the intricate balance of growth and protection in plants and its impact on their nutritional value due to low methionine levels under stress.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 1","pages":"87-100"},"PeriodicalIF":9.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TaWRKY55–TaPLATZ2 module negatively regulate saline–alkali stress tolerance in wheat","authors":"Lin Wei,&nbsp;Xinman Ren,&nbsp;Lumin Qin,&nbsp;Rong Zhang,&nbsp;Minghan Cui,&nbsp;Guangmin Xia,&nbsp;Shuwei Liu","doi":"10.1111/jipb.13793","DOIUrl":"10.1111/jipb.13793","url":null,"abstract":"<div>\u0000 \u0000 <p>Saline–alkaline soils are a major environmental problem that limit plant growth and crop productivity. Plasma membrane H⁺-ATPases and the salt overly sensitive (SOS) signaling pathway play important roles in plant responses to saline–alkali stress. However, little is known about the functional genes and mechanisms regulating the transcription of H⁺-ATPases and SOS pathway genes under saline–alkali stress. In the present study, we identified that the plant AT-rich sequence and zinc-binding (TaPLATZ2) transcription factor are involved in wheat response to saline–alkali stress by directly suppressing the expression of <i>TaHA2</i>/<i>TaSOS3</i>. The knockdown of <i>TaPLATZ2</i> enhances salt and alkali stress tolerance, while overexpression of <i>TaPLATZ2</i> leads to salt and alkali stress sensitivity in wheat. In addition, TaWRKY55 directly upregulated the expression of <i>TaPLATZ2</i> during saline–alkali stress. Through knockdown and overexpression of <i>TaWRKY55</i> in wheat, TaWRKY55 was shown to negatively modulate salt and alkali stress tolerance. Genetic analyses confirmed that <i>TaPLATZ2</i> functions downstream of <i>TaWRKY55</i> in response to salt and alkaline stresses. These findings provide a TaWRKY55–TaPLATZ2–<i>TaHA2/TaSOS3</i> regulatory module that regulates wheat responses to saline–alkali stress.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 1","pages":"19-34"},"PeriodicalIF":9.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13793","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass (Lolium perenne L.)","authors":"Guangjing Ma,&nbsp;Zhihao Liu,&nbsp;Shurui Song,&nbsp;Jing Gao,&nbsp;Shujie Liao,&nbsp;Shilong Cao,&nbsp;Yan Xie,&nbsp;Liwen Cao,&nbsp;Longxing Hu,&nbsp;Haichun Jing,&nbsp;Liang Chen","doi":"10.1111/jipb.13789","DOIUrl":"10.1111/jipb.13789","url":null,"abstract":"<p>Temperature sensitivity and tolerance play a key role in plant survival and production. Perennial ryegrass (<i>Lolium perenne</i> L.), widely cultivated in cool-season for forage supply and turfgrass, is extremely susceptible to high temperatures, therefore serving as an excellent grass for dissecting the genomic and genetic basis of high-temperature adaptation. In this study, expression analysis revealed that <i>LpHsfA2</i>, an important gene associated with high-temperature tolerance in perennial ryegrass, is rapidly and substantially induced under heat stress. Additionally, heat-tolerant varieties consistently display elevated expression levels of <i>LpHsfA2</i> compared with heat-sensitive ones. Comparative haplotype analysis of the <i>LpHsfA2</i> promoter indicated an uneven distribution of two haplotypes (<i>HsfA2</i>^Hap1 and <i>HsfA2</i>^Hap2) across varieties with differing heat tolerance. Specifically, the <i>HsfA2</i>^Hap1 allele is predominantly present in heat-tolerant varieties, while the <i>HsfA2</i>^Hap2 allele exhibits the opposite pattern. Overexpression of <i>LpHsfA2</i> confers enhanced thermotolerance, whereas silencing of <i>LpHsfA2</i> compromises heat tolerance. Furthermore, <i>LpHsfA2</i> orchestrates its protective effects by directly binding to the promoters of <i>LpHSP18.2</i> and <i>LpAPX1</i> to activate their expression, preventing the non-specific misfolding of intracellular protein and the accumulation of reactive oxygen species in cells. Additionally, LpHsfA4 and LpHsfA5 were shown to engage directly with the promoter of <i>LpHsfA2</i>, upregulating its expression as well as the expression of <i>LpHSP18.2</i> and <i>LpAPX1</i>, thus contributing to enhanced heat tolerance. Markedly, LpHsfA2 possesses autoregulatory ability by directly binding to its own promoter to modulate the self-transcription. Based on these findings, we propose a model for modulating the thermotolerance of perennial ryegrass by precisely regulating the expression of LpHsfA2. Collectively, these findings provide a scientific basis for the development of thermotolerant perennial ryegrass cultivars.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2346-2361"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13528","DOIUrl":"https://doi.org/10.1111/jipb.13528","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2077-2078"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13529","DOIUrl":"https://doi.org/10.1111/jipb.13529","url":null,"abstract":"<p>Pineapple, an important tropical herbaceous fruit tree, is renowned for its juicy composite fruits and distinctive aroma. Its extensive evolutionary history, primarily driven by vegetative propagation, has led to a highly heterozygous genome that has been difficult to fully resolve. Here, Feng et al. (pages 2208–2225) have successfully assembled the first telomere-to-telomere genome of pineapple, accompanied by a meticulously curated, highquality gene structure annotation. These comprehensive genomic resources provide a complete map for postgenomic research and breeding efforts in pineapple. The cover image features a flowering hybrid F1 plant, the result of a cross between BL and LY, two pineapple varieties used in the study.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"C1"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice","authors":"Lae-Hyeon Cho,&nbsp;Jinmi Yoon,&nbsp;Gibeom Baek,&nbsp;Win Tun,&nbsp;Hyeok Chan Kwon,&nbsp;Dae-Woo Lee,&nbsp;Seok-Hyun Choi,&nbsp;Yang-Seok Lee,&nbsp;Jong-Seong Jeon,&nbsp;Gynheung An","doi":"10.1111/jipb.13790","DOIUrl":"10.1111/jipb.13790","url":null,"abstract":"<p>Sucrose functions as a signaling molecule in several metabolic pathways as well as in various developmental processes. However, the molecular mechanisms by which sucrose regulates these processes remain largely unknown. In the present study, we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice. Exogenous application of sucrose promoted flowering by inducing florigen gene expression. Reduction of sucrose levels in the phloem through genetic modifications, such as the overexpression of the vacuolar invertase <i>OsVIN2</i> or the mutation of <i>OsSUT2</i>, a sucrose transporter, delayed flowering. Analysis of relative transcript levels of floral regulatory genes showed that sucrose activated <i>Ehd1</i> upstream of the florigen, with no significant effect on the expression of other upstream genes. Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded. The Ghd7 protein interacted with the E3 ligase IPA INTERACTING PROTEIN1 (IPI1), and sucrose-induced K48-linked polyubiquitination of Ghd7 via IPI1, leading to protein degradation. Mutants defective in IPI1 delayed flowering, confirming its role in modulating proteins involved in flowering. We conclude that sucrose acts as a signaling molecule to induce flowering by promoting Ghd7 degradation via IPI1.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2683-2700"},"PeriodicalIF":9.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why is pollen in Camellia oleifera inedible to honeybees?","authors":"Jannathan Mamut,&nbsp;Wei-Bing Zhang,&nbsp;Lu-Lu Tang","doi":"10.1111/jipb.13787","DOIUrl":"10.1111/jipb.13787","url":null,"abstract":"<p>This Commentary examines a recent study that addressed a long-standing controversy: Is the lethal effect of Tea-oil Camellia on honeybee larvae due to nectar or pollen toxicity? Flowers of <i>Camellia oleifera</i> are adapting to bird pollination, evolving ‘anti-bee’ traits such as theasaponin-containing pollen, which is toxic to bee larvae.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2307-2309"},"PeriodicalIF":9.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOS1 ubiquitinates SPL9 for degradation to modulate salinity-delayed flowering","authors":"Zhixin Jiao,&nbsp;Xiaoning Shi,&nbsp;Rui Xu,&nbsp;Mingxia Zhang,&nbsp;Leelyn Chong,&nbsp;Yingfang Zhu","doi":"10.1111/jipb.13784","DOIUrl":"10.1111/jipb.13784","url":null,"abstract":"<div>\u0000 \u0000 <p>Soil salinity is a serious environmental threat to plant growth and flowering. Flowering in the right place, at the right time, ensures maximal reproductive success for plants. Salinity-delayed flowering is considered a stress coping/survival strategy and the molecular mechanisms underlying this process require further studies to enhance the crop's salt tolerance ability. A nuclear pore complex (NPC) component, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1), has been recognized as a negative regulator of plant cold responses and flowering. Here, we challenged the role of HOS1 in regulating flowering in response to salinity stress. Interestingly, we discovered that HOS1 can directly interact with and ubiquitinate transcription factor SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9) to promote its protein degradation in response to salinity stress. Moreover, we demonstrated that <i>HOS1</i> and <i>SPL9</i> antagonistically regulate plant flowering under both normal and salt stress conditions. HOS1 was further shown to negatively regulate the expression of <i>SPLs</i> and several key flowering genes in response to salinity stress. These results jointly revealed that HOS1 is an important integrator in the process of modulating salinity-delayed flowering, thus offering new perspectives on a salinity stress coping strategy of plants.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2600-2612"},"PeriodicalIF":9.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13784","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The receptor-like cytoplasmic kinase OsBSK1-2 regulates immunity via an HLH/bHLH complex","authors":"Xun Wang,&nbsp;Zhijuan Diao,&nbsp;Chang Cao,&nbsp;Yan Liu,&nbsp;Na Xia,&nbsp;Youlian Zhang,&nbsp;Ling Lu,&nbsp;Fanyu Kong,&nbsp;Houli Zhou,&nbsp;Lizhe Chen,&nbsp;Jing Zhang,&nbsp;Bangsheng Wang,&nbsp;Ronghua Huang,&nbsp;Dingzhong Tang,&nbsp;Shengping Li","doi":"10.1111/jipb.13783","DOIUrl":"10.1111/jipb.13783","url":null,"abstract":"<p>Plants need to fine-tune defense responses to maintain a robust but flexible host barrier to various pathogens. Helix-loop-helix/basic helix-loop-helix (HLH/bHLH) complexes play important roles in fine-tuning plant development. However, the function of these genes in plant immunity and how they are regulated remain obscure. Here, we identified an atypical bHLH transcription factor, <i>Oryza sativa</i> (Os)HLH46, that interacts with rice receptor-like cytoplasmic kinase (RLCK) Os BRASSINOSTEROID-SIGNALING KINASE1-2 (OsBSK1-2), which plays a key role in rice blast resistance. OsBSK1-2 stabilized OsHLH46 both <i>in vivo</i> and <i>in vitro</i>. In addition, OsHLH46 positively regulates rice blast resistance, which depends on OsBSK1-2. OsHLH46 has no transcriptional activation activity and interacts with a typical bHLH protein, OsbHLH6, which negatively regulates rice blast resistance. OsbHLH6 binds to the promoter of <i>OsWRKY45</i> and inhibits its expression, while OsHLH46 suppresses the function of OsbHLH6 by blocking its DNA binding and transcriptional inhibition of <i>OsWRKY45</i>. Consistent with these findings, <i>OsWRKY45</i> was up-regulated in OsHLH46-overexpressing plants. In addition, the <i>oshlh46</i> mutant overexpressing OsbHLH6 is more susceptible to <i>Magnaporthe oryzae</i> than is the wild type, suggesting that OsHLH46 suppresses OsbHLH6-mediated rice blast resistance. Our results not only demonstrated that OsBSK1-2 regulates rice blast resistance via the OsHLH46/OsbHLH6 complex, but also uncovered a new mechanism for plants to fine-tune plant immunity by regulating the HLH/bHLH complex via RLCKs.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2754-2771"},"PeriodicalIF":9.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reading m6A marks in mRNA: A potent mechanism of gene regulation in plants","authors":"Thi Kim Hang Nguyen,&nbsp;Hunseung Kang","doi":"10.1111/jipb.13781","DOIUrl":"10.1111/jipb.13781","url":null,"abstract":"<p>Modifications to RNA have recently been recognized as a pivotal regulator of gene expression in living organisms. More than 170 chemical modifications have been identified in RNAs, with <i>N</i>⁶-methyladenosine (m⁶A) being the most abundant modification in eukaryotic mRNAs. The addition and removal of m⁶A marks are catalyzed by methyltransferases (referred to as “writers”) and demethylases (referred to as “erasers”), respectively. In addition, the m⁶A marks in mRNAs are recognized and interpreted by m⁶A-binding proteins (referred to as “readers”), which regulate the fate of mRNAs, including stability, splicing, transport, and translation. Therefore, exploring the mechanism underlying the m⁶A reader-mediated modulation of RNA metabolism is essential for a much deeper understanding of the epigenetic role of RNA modification in plants. Recent discoveries have improved our understanding of the functions of m⁶A readers in plant growth and development, stress response, and disease resistance. This review highlights the latest developments in m⁶A reader research, emphasizing the diverse RNA-binding domains crucial for m⁶A reader function and the biological and cellular roles of m⁶A readers in the plant response to developmental and environmental signals. Moreover, we propose and discuss the potential future research directions and challenges in identifying novel m⁶A readers and elucidating the cellular and mechanistic role of m⁶A readers in plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2586-2599"},"PeriodicalIF":9.3,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}