Journal of Integrative Plant Biology最新文献

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13522","DOIUrl":"https://doi.org/10.1111/jipb.13522","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 7","pages":"1261-1262"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13522","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-stabilized GIL1 suppresses PIN3 activity to inhibit hypocotyl gravitropism","authors":"Xiaolian Wang,&nbsp;Yanfang Yuan,&nbsp;Laurence Charrier,&nbsp;Zhaoguo Deng,&nbsp;Markus Geisler,&nbsp;Xing Wang Deng,&nbsp;Haodong Chen","doi":"10.1111/jipb.13736","DOIUrl":"10.1111/jipb.13736","url":null,"abstract":"<p>Light and gravity coordinately regulate the directional growth of plants. Arabidopsis Gravitropic in the Light 1 (GIL1) inhibits the negative gravitropism of hypocotyls in red and far-red light, but the underlying molecular mechanisms remain elusive. Our study found that GIL1 is a plasma membrane-localized protein. In endodermal cells of the upper part of hypocotyls, GIL1 controls the negative gravitropism of hypocotyls. GIL1 directly interacts with PIN3 and inhibits the auxin transport activity of PIN3. Mutation of <i>PIN3</i> suppresses the abnormal gravitropic response of <i>gil1</i> mutant. The GIL1 protein is unstable in darkness but it is stabilized by red and far-red light. Together, our data suggest that light-stabilized GIL1 inhibits the negative gravitropism of hypocotyls by suppressing the activity of the auxin transporter PIN3, thereby enhancing the emergence of young seedlings from the soil.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1886-1897"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13736","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9","authors":"Chenglu Zhao,&nbsp;Huanhuan Lou,&nbsp;Qian Liu,&nbsp;Siqi Pei,&nbsp;Qiansheng Liao,&nbsp;Zhenghe Li","doi":"10.1111/jipb.13741","DOIUrl":"10.1111/jipb.13741","url":null,"abstract":"<p>Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2079-2082"},"PeriodicalIF":9.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PE6c greatly enhances prime editing in transgenic rice plants","authors":"Zhenghong Cao,&nbsp;Wei Sun,&nbsp;Dexin Qiao,&nbsp;Junya Wang,&nbsp;Siyun Li,&nbsp;Xiaohan Liu,&nbsp;Cuiping Xin,&nbsp;Yu Lu,&nbsp;Syeda Leeda Gul,&nbsp;Xue-Chen Wang,&nbsp;Qi-Jun Chen","doi":"10.1111/jipb.13738","DOIUrl":"10.1111/jipb.13738","url":null,"abstract":"<p>Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding. Four new types of prime editors (PEs) named PE6a–d were recently generated using evolved and engineered reverse transcriptase (RT) variants from three different sources. In this study, we tested the editing efficiencies of four PE6 variants and two additional PE6 constructs with double-RT modules in transgenic rice (<i>Oryza sativa</i>) plants. PE6c, with an evolved and engineered RT variant from the yeast Tf1 retrotransposon, yielded the highest prime-editing efficiency. The average fold change in the editing efficiency of PE6c compared with PEmax exceeded 3.5 across 18 agronomically important target sites from 15 genes. We also demonstrated the feasibility of using two RT modules to improve prime-editing efficiency. Our results suggest that PE6c or its derivatives would be an excellent choice for prime editing in monocot plants. In addition, our findings have laid a foundation for prime-editing-based breeding of rice varieties with enhanced agronomically important traits.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1864-1870"},"PeriodicalIF":9.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13738","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.","authors":"Weizhi He,&nbsp;Hang Liu,&nbsp;Zhangkuanyu Wu,&nbsp;Qing Miao,&nbsp;Xinyi Hu,&nbsp;Xin Yan,&nbsp;Hangyu Wen,&nbsp;Yaojie Zhang,&nbsp;Xueqing Fu,&nbsp;Li Ren,&nbsp;Kexuan Tang,&nbsp;Ling Li","doi":"10.1111/jipb.13708","DOIUrl":"10.1111/jipb.13708","url":null,"abstract":"<p>The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood (<i>Artemisia annua</i> L.). Light was previously shown to promote artemisinin production, but the underlying regulatory mechanism remains elusive. In this study, we demonstrate that ELONGATED HYPOCOTYL 5 (HY5), a central transcription factor in the light signaling pathway, cannot promote artemisinin biosynthesis on its own, as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their transcription. Transcriptome analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with AaHY5. <i>AaBBX21</i> showed a trichome-specific expression pattern. Additionally, the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes <i>AaGSW1</i>, <i>AaMYB108</i>, and <i>AaORA</i>, encoding positive regulators of artemisinin biosynthesis. Moreover, AaHY5 and AaBBX21 physically interacted with the <i>A. annua</i> E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1). In the dark, AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex, explaining the enhanced production of artemisinin upon light exposure. Our study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 8","pages":"1735-1751"},"PeriodicalIF":9.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of vacuolar phosphate efflux supporting soybean root hair growth in response to phosphate deficiency","authors":"Zhong Shan,&nbsp;Yanli Chu,&nbsp;Guangfang Sun,&nbsp;Rui Chen,&nbsp;Jun Yan,&nbsp;Qiwei He,&nbsp;Yingna Liu,&nbsp;Bin Wang,&nbsp;Mingda Luan,&nbsp;Wenzhi Lan","doi":"10.1111/jipb.13735","DOIUrl":"10.1111/jipb.13735","url":null,"abstract":"<div>\u0000 \u0000 <p>Phosphorus is an essential macronutrient for plant growth and development. In response to phosphate (Pi) deficiency, plants rapidly produce a substitutive amount of root hairs; however, the mechanisms underlying Pi supply for root hair growth remain unclear. Here, we observed that soybean (<i>Glycine max</i>) plants maintain a consistent level of Pi within root hairs even under external Pi deficiency. We therefore investigated the role of vacuole-stored Pi, a major Pi reservoir in plant cells, in supporting root hair growth under Pi-deficient conditions. Our findings indicated that two vacuolar Pi efflux (VPE) transporters, GmVPE1 and GmVPE2, remobilize vacuolar stored Pi to sustain cytosolic Pi content in root hair cells. Genetic analysis showed that double mutants of <i>GmVPE1</i> and <i>GmVPE2</i> exhibited reduced root hair growth under low Pi conditions. Moreover, <i>GmVPE1</i> and <i>GmVPE2</i> were highly expressed in root hairs, with their expression levels significantly upregulated by low Pi treatment. Further analysis revealed that GmRSL2 (ROOT HAIR DEFECTIVE 6-like 2), a transcription factor involved in root hair morphogenesis, directly binds to the promoter regions of <i>GmVPE1</i> and <i>GmVPE2</i>, and promotes their expressions under low Pi conditions. Additionally, mutants lacking both <i>GmRSL2</i> and its homolog <i>GmRSL3</i> exhibited impaired root hair growth under low Pi stress, which was rescued by overexpressing either <i>GmVPE1</i> or <i>GmVPE2</i>. Taken together, our study has identified a module comprising vacuolar Pi exporters and transcription factors responsible for remobilizing vacuolar Pi to support root hair growth in response to Pi deficiency in soybean.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1983-1999"},"PeriodicalIF":9.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halotolerant Bacillus sp. strain RA coordinates myo-inositol metabolism to confer salt tolerance to tomato","authors":"Fenghui Wu,&nbsp;Zengting Chen,&nbsp;Xiaotong Xu,&nbsp;Xin Xue,&nbsp;Yanling Zhang,&nbsp;Na Sui","doi":"10.1111/jipb.13733","DOIUrl":"10.1111/jipb.13733","url":null,"abstract":"<div>\u0000 \u0000 <p>Soil salinity is a worldwide problem threatening crop yields. Some plant growth-promoting rhizobacteria (PGPR) could survive in high salt environment and assist plant adaptation to stress. Nevertheless, the genomic and metabolic features, as well as the regulatory mechanisms promoting salt tolerance in plants by these bacteria remain largely unknown. In the current work, a novel halotolerant PGPR strain, namely, <i>Bacillus</i> sp. strain RA can enhance tomato tolerance to salt stress. Comparative genomic analysis of strain RA with its closely related species indicated a high level of evolutionary plasticity exhibited by strain-specific genes and evolutionary constraints driven by purifying selection, which facilitated its genomic adaptation to salt-affected soils. The transcriptome further showed that strain RA could tolerate salt stress by balancing energy metabolism via the reprogramming of biosynthetic pathways. Plants exude a plethora of metabolites that can strongly influence plant fitness. The accumulation of <i>myo</i>-inositol in leaves under salt stress was observed, leading to the promotion of plant growth triggered by <i>Bacillus</i> sp. strain RA. Importantly, <i>myo</i>-inositol serves as a selective force in the assembly of the phyllosphere microbiome and the recruitment of plant-beneficial species. It promotes destabilizing properties in phyllosphere bacterial co-occurrence networks, but not in fungal networks. Furthermore, interdomain interactions between bacteria and fungi were strengthened by <i>myo</i>-inositol in response to salt stress. This work highlights the genetic adaptation of RA to salt-affected soils and its ability to impact phyllosphere microorganisms through the adjustment of <i>myo</i>-inositol metabolites, thereby imparting enduring resistance against salt stress in tomato.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1871-1885"},"PeriodicalIF":9.3,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus","authors":"Jialing Fu,&nbsp;Li Liao,&nbsp;Jiajing Jin,&nbsp;Zhihao Lu,&nbsp;Juan Sun,&nbsp;Lizhi Song,&nbsp;Yue Huang,&nbsp;Shengjun Liu,&nbsp;Ding Huang,&nbsp;Yuantao Xu,&nbsp;Jiaxian He,&nbsp;Bin Hu,&nbsp;Yiqun Zhu,&nbsp;Fangfang Wu,&nbsp;Xia Wang,&nbsp;Xiuxin Deng,&nbsp;Qiang Xu","doi":"10.1111/jipb.13719","DOIUrl":"10.1111/jipb.13719","url":null,"abstract":"<p>Dwarfing is a pivotal agronomic trait affecting both yield and quality. Citrus species exhibit substantial variation in plant height, among which internode length is a core element. However, the molecular mechanism governing internode elongation remains unclear. Here, we unveiled that the transcriptional cascade consisting of <i>B-BOX DOMAIN PROTEIN 22</i> (<i>BBX22</i>) and <i>ELONGATED HYPOCOTYL 5</i> (<i>HY5</i>) finely tunes plant height and internode elongation in citrus. Loss-of-function mutations of <i>BBX22</i> in an early-flowering citrus (<i>Citrus hindsii</i> “SJG”) promoted internode elongation and reduced pigment accumulation, whereas ectopic expression of <i>BBX22</i> in SJG, sweet orange (<i>C. sinensis</i>), pomelo (<i>C. maxima</i>) or heterologous expression of <i>BBX22</i> in tomato (<i>Solanum lycopersicum</i>) significantly decreased internode length. Furthermore, exogenous application of gibberellin A3 (GA₃) rescued the shortened internode and dwarf phenotype caused by <i>BBX22</i> overexpression. Additional experiments revealed that BBX22 played a dual role in regulation internode elongation and pigmentation in citrus. On the one hand, it directly bound to and activated the expression of <i>HY5</i>, GA metabolism gene (<i>GA2 OXIDASE 8</i>, <i>GA2ox8</i>), carotenoid biosynthesis gene (<i>PHYTOENE SYNTHASE 1</i>, <i>PSY1</i>) and anthocyanin regulatory gene (<i>Ruby1,</i> a MYB DOMAIN PROTEIN). On the other hand, it acted as a cofactor of HY5, enhancing the ability of HY5 to regulate target genes expression. Together, our results reveal the critical role of the transcriptional cascade consisting of <i>BBX22</i> and <i>HY5</i> in controlling internode elongation and pigment accumulation in citrus. Unraveling the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 8","pages":"1752-1768"},"PeriodicalIF":9.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141496518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo haploid induction in cauliflower, kale, and broccoli","authors":"Guixiang Wang,&nbsp;Mei Zong,&nbsp;Shuo Han,&nbsp;Hong Zhao,&nbsp;Mengmeng Duan,&nbsp;Xin Liu,&nbsp;Ning Guo,&nbsp;Fan Liu","doi":"10.1111/jipb.13730","DOIUrl":"10.1111/jipb.13730","url":null,"abstract":"<p>Modifying the centromeric histone <i>CENH3</i> or <i>PHOSPHOLIPASE</i> <i>D</i> genes in cauliflower (<i>Brassica oleracea var. botrytis</i>) created haploid induction lines, which can be widely used for in vivo haploid induction in cauliflower, kale, and broccoli, thus enabling rapid utilization of germplasm resources and improving breeding efficiency.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1823-1826"},"PeriodicalIF":9.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pair of nuclear factor Y transcription factors act as positive regulators in jasmonate signaling and disease resistance in Arabidopsis","authors":"Chuyu Lin,&nbsp;Chenghao Lan,&nbsp;Xiaoxiao Li,&nbsp;Wei Xie,&nbsp;Fucheng Lin,&nbsp;Yan Liang,&nbsp;Zeng Tao","doi":"10.1111/jipb.13732","DOIUrl":"10.1111/jipb.13732","url":null,"abstract":"<div>\u0000 \u0000 <p>The plant hormone jasmonate (JA) regulates plant growth and immunity by orchestrating a genome-wide transcriptional reprogramming. In the resting stage, JASMONATE-ZIM DOMAIN (JAZ) proteins act as main repressors to regulate the expression of JA-responsive genes in the JA signaling pathway. However, the mechanisms underlying de-repression of JA-responsive genes in response to JA treatment remain elusive. Here, we report two nuclear factor Y transcription factors NF-YB2 and NF-YB3 (thereafter YB2 and YB3) play key roles in such de-repression in <i>Arabidopsis</i>. YB2 and YB3 function redundantly and positively regulate plant resistance against the necrotrophic pathogen <i>Botrytis cinerea</i>, which are specially required for transcriptional activation of a set of JA-responsive genes following inoculation. Furthermore, YB2 and YB3 modulated their expression through direct occupancy and interaction with histone demethylase Ref6 to remove repressive histone modifications. Moreover, YB2 and YB3 physically interacted with JAZ repressors and negatively modulated their abundance, which in turn attenuated the inhibition of JAZ proteins on the transcription of JA-responsive genes, thereby activating JA response and promoting disease resistance. Overall, our study reveals the positive regulators of YB2 and YB3 in JA signaling by positively regulating transcription of JA-responsive genes and negatively modulating the abundance of JAZ proteins.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"2042-2057"},"PeriodicalIF":9.3,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13732","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}