Journal of Integrative Plant Biology最新文献

{"title":"The targeted metabolomic profile of laticifers in rubber tree.","authors":"Xiaomin Deng, Shuguang Yang, Qiang Gao, Yanling Chen, Xia Zeng, Minjing Shi, Shaohua Wu, Weimin Tian, Xuchu Wang, Jinquan Chao","doi":"10.1111/jipb.13948","DOIUrl":"10.1111/jipb.13948","url":null,"abstract":"<p><p>Targeted metabolomic profiling of rubber tree (Hevea brasiliensis) laticifers identified metabolites that were reprogramming by domestication, revealed active isoprenoid metabolism in the laticifers, and discovered loci with potential biosynthetic applications, supporting the potential of developing laticifers as bioreactors for production of valuable metabolites in Hevea.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"852-854"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13084202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytosterols: Structural variations, biosynthetic pathways, and their biological roles.","authors":"Chao Tan, Shuzhen Men","doi":"10.1111/jipb.70135","DOIUrl":"10.1111/jipb.70135","url":null,"abstract":"<p><p>Phytosterols are a diverse class of isoprenoid-derived lipids that serve as essential structural components of plant membranes and regulators of growth and reproduction. Unlike animals and fungi, which predominantly utilize cholesterol and ergosterol, plants produce a complex array of over 250 sterol molecules. These include major forms such as β-sitosterol, stigmasterol, and campesterol as well as minor components like cholesterol and various sterol biosynthetic intermediates. This review provides a comprehensive overview of plant sterols, first addressing their structural diversity and distribution across species and tissues, and then exploring their biosynthesis, transport, and functions. A key focus is placed on their role as membrane modulators, influencing fluidity, permeability, and the formation of lipid rafts. Finally, we synthesize genetic and molecular evidence, demonstrating the critical functions of sterols and their derivatives in both reproductive and vegetative development. We conclude by highlighting persistent gaps in our knowledge and proposing future research directions to unravel the multifaceted roles of these essential molecules.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"942-966"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing of medicinal plants: Advances, challenges, and prospects.","authors":"Wenhua Chen, Yi Shi, Zongyou Lv, Wansheng Chen","doi":"10.1111/jipb.70110","DOIUrl":"10.1111/jipb.70110","url":null,"abstract":"<p><p>Medicinal plants produce important pharmaceuticals, but these compounds are often present at low levels or only in specific tissues; in addition, many medicinal plants produce small amounts of biomass and are difficult to cultivate. Genome editing for agronomic traits and metabolic engineering holds promise for improving pharmaceutical production, and genome-editing applications in medicinal plants have expanded as genome-editing techniques have advanced. For example, genome editing has been used to regulate the production of phenolic acids and tanshinone metabolites of Salvia miltiorrhiza in medicinal plants. In this review, we synthesize the current knowledge on the development and applications of gene-editing tools in medicinal plants. Furthermore, we summarize the limitations of genome editing in these species and propose solutions for addressing these challenges to fully harness this technology for improving these important plants. We focus on novel technologies to enhance the regeneration rates of transgenic plants, artificial intelligence-assisted multiomics approaches for predicting editing efficiency, key components that optimize genome-editing efficacy, and the development of innovative gene-editing systems. Finally, we offer perspectives on advancing metabolic engineering strategies for medicinal plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"903-925"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering prime editors in Salvia miltiorrhiza for precise genome modification.","authors":"Qi Yao, Yi Ye, Muyao Yu, Yifu Tian, Qi Liu, Han Zheng, Luqi Huang","doi":"10.1111/jipb.70006","DOIUrl":"https://doi.org/10.1111/jipb.70006","url":null,"abstract":"<p><p>An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevated contents of the bioactive abietane diterpene tanshinone, advancing synthetic biology applications in medicinal plants.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 4","pages":"855-857"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in plant natural products: Biosynthesis, bioengineering, and applications.","authors":"Alisdair R Fernie, Guoyin Kai, Jie Luo","doi":"10.1111/jipb.70242","DOIUrl":"10.1111/jipb.70242","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"845-848"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting the biosynthesis, regulation, and metabolic engineering of steroidal glycoalkaloids in tomato.","authors":"Jiayi Chen, Mingchun Liu, Yang Zhang, Feng Bai","doi":"10.1111/jipb.70077","DOIUrl":"10.1111/jipb.70077","url":null,"abstract":"<p><p>Steroidal glycoalkaloids (SGAs) are predominantly found in Solanaceous plants, including tomato (Solanum lycopersicum). In addition to their roles in resistance to herbivores, pathogens, and environmental stresses, SGAs exert antifungal, antibacterial, and anticancer effects. Over the past 15 years, the biosynthesis pathway of SGAs in tomato has been progressively investigated. A growing number of intermediate compounds and novel biosynthetic enzymes have been identified. In addition, various regulatory factors and their underlying regulatory mechanisms governing SGAs biosynthesis have been increasingly elucidated. Building upon these advances in understanding the SGAs biosynthetic pathway and its regulatory network, metabolic engineering of the SGAs pathway in tomato has been achieved using techniques such as gene editing. This mini review summarizes the current understanding of SGAs biosynthesis and regulatory mechanisms in tomato, and provides an overview of recent progress and future perspectives in metabolic engineering applications targeting this pathway.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"861-868"},"PeriodicalIF":9.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145538464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TADs, CGVs, and compartmentalization in genomes: Providing a new way for crop domestication and improvement.","authors":"Qamar U Zaman, Robert J Henry, Zhihua Mu, Shuangqian Shen, Jie Luo, Rajeev K Varshney","doi":"10.1111/jipb.70240","DOIUrl":"https://doi.org/10.1111/jipb.70240","url":null,"abstract":"<p><p>Genetic variation underlying phenotypic diversity between wild and domesticated species has been extensively studied, the contribution of higher-order chromatin architecture to these processes remains less explored. Advances in Hi-C and related genomic technologies have revealed that plant genomes exhibit complex three-dimensional (3D) genome organization, hierarchically structured into A/B compartments, and topologically associated domains (TADs). TADs represent self-interacting genomic regions that can constrain or regulate without directly determining transcriptional outcomes. Alterations to TAD organization or boundary have been associated with changes in chromatin interactions and gene regulatory potential in specific developmental or environmental contexts. In plants, emerging evidence indicates that TAD structure can be genetically and environmentally modulated, despite the absence of canonical architectural proteins such as CTCF. Both environmental stress and genetic perturbations have been shown to remodel chromatin organization, with context-dependent changes in gene expression. Such plasticity in chromatin dynamics that contribute to adaptive responses raises a potential link between 3D genomic structure and cryptic genetic variations (CGVs). CGVs remain phenotypically silent under normal conditions but can be revealed under environmental or genetic perturbations, representing an additional layer of regulatory potential in plant genomes. Here, we propose that stress-induced chromatin organization, including changes in TAD organization and chromatin compartmentalization, may influence accessibility and expression of CGVs in a context-dependent manner. While a direct mechanistic link between TADs and CGVs remains largely unexplored. Here, we reviewed recent findings from model plants and major crops to highlight how variation in 3D genome organization can contribute to transcriptional plasticity, stress responses, and lineage-specific regulatory evolution. By integrating 3D genomics, chromatin accessibility, and multi-omics data, we outline a conceptual framework for generating hypotheses and open questions on how TAD-associated chromatin dynamics and CGVs together may shape transcriptional plasticity, stress responses, and long-term adaptive evolution in plants with implications for future crop improvement strategies.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal effector FolCP1b promotes infection by sequestering host subtilase SlSBT1 to protect apoplastic effectors from degradation.","authors":"Zhengang Miao, Baoshan Wang, Limin Song, Wenxing Liang","doi":"10.1111/jipb.70244","DOIUrl":"https://doi.org/10.1111/jipb.70244","url":null,"abstract":"<p><p>Plant pathogens use a diverse arsenal of effectors to suppress host immunity, though the precise mechanisms of their action are often not fully understood. In this study, we characterize FolCP1b, a cerato-platanin (CP) effector secreted by Fusarium oxysporum f. sp. lycopersici (Fol), as a key intracellular virulence factor that disrupts host defenses and protects other Fol effectors. FolCP1b interacts with the host apoplastic subtilase SlSBT1 within the plant cytoplasm, leading to its intracellular retention and preventing its secretion to the apoplast. As a result, SlSBT1-mediated degradation of key Fol effectors, such as FolEP1 and FolEP2, is impaired, thereby promoting Fol infection. Unlike canonical protease inhibitors, FolCP1b operates by altering host protein subcellular localization rather than inhibiting enzymatic activity. Our findings unveil a novel \"effector hijacking\" mechanism, through which one intracellular effector safeguards apoplastic effectors from host proteolytic degradation, thereby enhancing fungal pathogenicity.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"12-hydroxylauric acid, a novel growth regulator, promotes plant organ development.","authors":"Yan Peng, Mengling Zhang, Zengdong Tan, Hongxiang Lou, Bao Yang, Yuting Zhang, Zhicong Zhao, Ruyi Fan, Haiyan Fan, Jie Chen, Xuan Yao, Shaoping Lu, Hu Zhao, Liang Guo, Kede Liu","doi":"10.1111/jipb.70243","DOIUrl":"https://doi.org/10.1111/jipb.70243","url":null,"abstract":"<p><p>Crop yield is largely determined by the size of harvestable organs and understanding the mechanisms that govern organ size is therefore crucial for improving crop productivity. CYP78As are a plant-specific subfamily of cytochrome P450 enzymes that have been identified as organ size regulators and are expressed in various plant organs. We previously identified BnaA09.CYP78A9, whose expression is markedly upregulated in long-silique cultivars by a CACTA-like transposable element (CTE) insertion in the regulatory region, acts as a pleiotropic regulator of yield-related traits in long-silique rapeseed varieties. Here, we show that BnaA09.CYP78A9^+CTE is expressed predominantly in the siliques and seeds of rapeseed cultivar ZS11. cyp78a9-CRISPR knockout mutants exhibited reductions in silique length, seed size, and seed number per silique. Enzyme assays revealed that BnaA09.CYP78A9 converts lauric acid (LA) to 12-hydroxylauric acid (12-HOLA), and endogenous metabolite quantification revealed that 12-HOLA levels were 5.01-fold greater in long-silique materials versus short-silique materials. Application of exogenous 12-HOLA significantly increased silique/pod elongation and seed weight, enhancing yield per plant by 32.77% in Arabidopsis and yield per unit area by 7.91%-30.82% in rapeseed and 30.14% in soybean compared with controls, respectively. 12-HOLA application also stimulated fruit expansion in horticultural crops, increasing fresh fruit weight by 20.64%-22.96% in cucumber and 11.92%-24.13% in tomato. Transcriptome analyses revealed that 12-HOLA treatment upregulated the expression of genes involved in auxin biosynthesis, transport, and signaling. 12-HOLA treatment not only rapidly activated numerous transcription factors but also significantly promoted carbon metabolism within rapeseed siliques. Our study provides strong evidence that 12-HOLA produced by BnaA09.CYP78A9 activates the auxin pathway, promotes cell elongation, and increases fruit size and seed weight. These findings highlight the potential use of 12-HOLA as a natural plant growth regulator and CYP78A9 as a target for gene editing and molecular breeding.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conserved fungal Egh16-like effector suppresses host defense by disrupting ATP binding of wheat MPK3.","authors":"Jiangang Kang, Gang Niu, Yihui Liao, Daiying Xu, Huaijian Xu, Qing Yang, Daiyuan Sun, Kaili Duan, Yang Yang, Chenfang Wang, Qinhu Wang, Huiquan Liu, Jin-Rong Xu, Cong Jiang","doi":"10.1111/jipb.70241","DOIUrl":"https://doi.org/10.1111/jipb.70241","url":null,"abstract":"<p><p>ATP binding is an essential event in diverse biological processes including plant immunity. The ATP-binding domains in plant kinases share similar structural properties, providing a potential common target for pathogens. However, effectors targeting the ATP-binding domains to modulate kinase activity have not been identified. In this study, we identified a conserved effector containing an Egh16-like domain (Cee1) in Fusarium graminearum. As an in planta induced gene, CEE1 plays a stage-specific role in infectious growth within wheat rachis. Upon translocation into plant cells, Cee1 interacts with the ATP-binding domain of TaMPK3 via its Egh16-like domain. This interaction interferes with the ATP binding and impairs the kinase activity of TaMPK3, leading to reduced phosphorylation levels of TaWRKY33 and subsequent inactivation of downstream resistance responses. F. graminearum harbors three paralogs of Cee1, each containing two adjacent motifs responsible for specific interaction with ATP-binding pockets, all crucial for pathogenesis. The quadruple mutant lacking these four CEE genes shows drastically reduced pathogenicity, and CEE genes have been identified as silencing targets for improving wheat FHB resistance. Taken together, Cee1 and its paralogs act as core effectors in F. graminearum by targeting the ATP-binding domains of plant kinases, demonstrating the representative mode of action of the Egh16-like domain in fungal-plant interactions.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}