Journal of Integrative Plant Biology最新文献

{"title":"Engineer the eukaryotic OMEGA−Fanzor systems for genome editing in plants","authors":"Yuan Ji,&nbsp;Yan Sun,&nbsp;Hejie Zhou,&nbsp;Zimeng Liu,&nbsp;Ziran Sun,&nbsp;Gencheng Xu,&nbsp;Hongzhi Wen,&nbsp;Zerong Zheng,&nbsp;Lixiang Tu,&nbsp;Zitong Yang,&nbsp;Yuanyan Zhang,&nbsp;Xi Liu,&nbsp;Shirong Zhou,&nbsp;Xiaoou Dong,&nbsp;Yanpeng Wang,&nbsp;Chao Li,&nbsp;Jianmin Wan","doi":"10.1111/jipb.70049","DOIUrl":"10.1111/jipb.70049","url":null,"abstract":"<p>The activity of the eukaryotic OMEGA-Fanzor genome editing system remains limited in plants. We engineered the Fanzor nucleases SpuFz1, GtFz1, NlovFz2, and MmeFz2 in plants, with NlovFz2 being the most efficient, achieving up to 50.0% editing in regenerated rice plants, making it a promising tool for plant genome editing.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3160-3162"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a robust multiplex CRISPR/Cas12i3-5M system for trait stacking in soybean","authors":"Wenxin Lin,&nbsp;Haijie Wu,&nbsp;Huaqin Kuang,&nbsp;Xiangchi Feng,&nbsp;Mengyan Bai,&nbsp;Fanghui He,&nbsp;Ruixuan Liang,&nbsp;Yizhi Zeng,&nbsp;Meina Li,&nbsp;Fanjiang Kong,&nbsp;Baohui Liu,&nbsp;Yuefeng Guan","doi":"10.1111/jipb.70233","DOIUrl":"10.1111/jipb.70233","url":null,"abstract":"<p>The high-efficiency multiplex gene editing technology based CRISPR-Cas12i3-5M is capable of simultaneously editing 13 target sites in soybean, and was used to generate germplasm with high oleic acid content and no beany flavor.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3172-3174"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized Cas-SF01 gene-editing toolbox shortens flowering timing in commercial maize inbred JING724","authors":"Mengyuan Liu,&nbsp;Yao Wang,&nbsp;Lu Zhang,&nbsp;Xiang Zhang,&nbsp;Xiaqing Wang,&nbsp;Xinxiang Liu,&nbsp;Yue Fu,&nbsp;Xiaoxu Li,&nbsp;Ziheng Song,&nbsp;Ya Liu,&nbsp;Ronghuan Wang,&nbsp;Jiuran Zhao","doi":"10.1111/jipb.70264","DOIUrl":"10.1111/jipb.70264","url":null,"abstract":"<p>The gene-editing tool Cas-SF01 was optimized to maximize its efficiency in maize. The Cas-SF01-TREX2 configuration was superior in enabling high-purity gene mutations. This toolkit enabled commercial maize to flower seven days earlier without yield loss, thereby securing harvests and accelerating crop breeding.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3178-3180"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A surrogate-selection-enhanced TnpBmax platform enables efficient genome editing in rice and tomato","authors":"Enze Li,&nbsp;Jiyong Xie,&nbsp;Xinbo Li,&nbsp;Zai Zheng,&nbsp;Mugui Wang,&nbsp;Jian-Kang Zhu,&nbsp;Yifu Tian","doi":"10.1111/jipb.70159","DOIUrl":"10.1111/jipb.70159","url":null,"abstract":"<p>TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3166-3168"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel effector HYPB1 of cotton bollworm (Helicoverpa armigera) inhibits biosynthesis of plant secondary metabolites and promotes feeding by targeting cotton dirigent protein GhDIR15.","authors":"Yaxin Wang, Chuanying Zhu, Ying Wang, Peng Han, Xueke Li, Gefei Chen, Ying Wang, Muna Alariqi, Zhongping Xu, Qiongqiong Wang, Fuqiu Wang, Yan Zhang, Lianlian Che, Amjad Hussain, Xinhui Nie, Wei Gao, Xianlong Zhang, Longfu Zhu, Shuangxia Jin","doi":"10.1111/jipb.70270","DOIUrl":"10.1111/jipb.70270","url":null,"abstract":"<p><p>Herbivore effectors play central roles in plant-insect interactions; yet, their molecular targets and modes of action remain poorly defined. Here, we performed data-independent acquisition proteomic profiling of oral secretions from cotton bollworm (Helicoverpa armigera) larvae fed on an artificial diet and four cotton cultivars. A total of 212 proteins were identified, including 39 differentially expressed proteins and 13 candidate effectors. Based on secretion characteristics and evolutionary features, six venom protein-related candidates were selected for functional validation. Transgenic cotton plants overexpressing these genes were generated, and feeding assays demonstrated that three independent 35S:PESD3 lines and three 35S:HYPB1 lines significantly enhanced bollworm performance relative to wild-type cotton. Further analyses showed that HYPB1 and PESD3 can be secreted into cotton tissues through mechanical wounds. Among these candidates, HYPB1 showed typical structural and evolutionary characteristics of venom-related proteins. Multiple complementary protein-protein interaction assays demonstrated that HYPB1 physically interacts with the cotton dirigent protein GhDIR15. Silencing of GhDIR15 via virus-induced gene silencing reduced cotton resistance to H. armigera and was accompanied by decreased lignin accumulation and reduced phenolic metabolite levels, indicating suppression of the cell wall-associated defense pathway. Together, these results identify HYPB1 as a previously uncharacterized effector that promotes bollworm feeding by targeting GhDIR15 and suppressing lignin biosynthesis, thereby further compromising cell wall-mediated defense. Although PESD3 also promoted bollworm performance in transgenic cotton, its underlying mechanism requires further investigation. This work provides mechanistic insight into how H. armigera manipulates host secondary metabolism to attenuate plant defense.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3473-3489"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A group of TCP transcription factors is a missing link in strigolactone signaling.","authors":"Yi Huang, Lumei Bi, Yongshuang Huang, Jinlan Liu, Lin Wang, Fang Qiu, Yuehua Wang, Li Chen, Meng Zhang, Ruifeng Yao","doi":"10.1111/jipb.70281","DOIUrl":"10.1111/jipb.70281","url":null,"abstract":"<p><p>Strigolactones (SLs) are plant-specialized butenolide signaling molecules, recognized as endogenous plant hormones, that control plant development and environmental adaptation. In Arabidopsis (Arabidopsis thaliana), the repressor D53-like SMXLs regulate the expression of a vast number of genes in an EAR-motif-dependent manner to mediate SL signaling. However, it remains unclear how the SMXLs are recruited to specific genes and implement unique functions in vivo. Based on chromatin co-distribution analysis, we constructed a chromatin co-localization map of SMXL6 with 108 transcription factors. Among the candidate transcription factors, the Class II TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family member TCP4 shows the highest frequency of chromatin co-localization with SMXL6. SMXL6 and TCP4 co‑localize at the promoter regions of 18 SL-induced SMXL6 target genes (SISGs), including BRC1. We confirmed that TCP4 interacts with SMXL6 and can bind directly to these co‑localized sites. The loss of CIN-TCPs function reduces the hormone responsiveness of the SL-induced genes. Introducing the tcp3/4/10 into SL‑deficient mutants restored the BRC1 expression to a level exceeding that of the wild type. However, the branching phenotype of the SL‑deficient mutant was only partially rescued, suggesting a limited role for BRC1 in SL‑mediated branching control and implicating the involvement of additional factors. An unexpected finding was that tcp3/4/10 rescued the dwarf phenotype of the SL‑deficient mutants, providing an opportunity to elucidate the mechanisms underlying SL‑regulated plant height. These findings demonstrate that TCP4 mediates SMXL6 chromatin recruitment during SL signaling, and provide a new understanding of how SMXL6 participates in SL signaling-mediated gene expression and plant development.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3426-3439"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing precisely boosts salt-tolerant japonica rice yield.","authors":"Mengmeng Zhou, Qihao Sun, Jian Sun, Jiaoyan An, Yanpeng Lv, Cui Li, Yunlong Lu, Jiyue Qiao, Xiaoming Zheng, Qian Qian","doi":"10.1111/jipb.70313","DOIUrl":"10.1111/jipb.70313","url":null,"abstract":"<p><p>Gene editing of three key genes, G PROTEIN GAMMA SUBUNIT, CYTOKININ OXIDASE, AND IDEAL PLANT ARCHITECTURE 1, improved the salt-tolerant japonica rice variety Lansheng increased yield while maintaining its original salt tolerance and quality.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3295-3297"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A GmOIL2 allele separates fertility and seed traits in soybean.","authors":"Ying Chen, Jing-Tian Wang, De-Hui Yuan, Yi-Lin Zeng, Xiao-Shuang Ma, Xiu-Ting Liang, Qiu-Yun Jiang, Yi-Yi Xia, Jian-Fang Zuo, Yuan-Ming Zhang","doi":"10.1111/jipb.70345","DOIUrl":"10.1111/jipb.70345","url":null,"abstract":"<p><p>A CRISPR-derived 36-bp in-frame deletion in GmOIL2 maintains fertility and seed weight while altering seed-related traits. This allele provides a genetic resource for improving soybean quality without affecting seed weight.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3301-3303"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148409838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient transformation and gene editing system for Lilium species.","authors":"Yajie Zhao, Yixuan Li, Jinxin Shi, Yutong Chen, Jingwei Wei, Jinlong Li, Yiqing Wang, Dingyi Liu, Dong Jing, Shuo Long, Manman Zhang, Junwei Tang, Jian Wu","doi":"10.1111/jipb.70237","DOIUrl":"10.1111/jipb.70237","url":null,"abstract":"<p><p>A transformation system exploits lily bulb scale propagation and de novo formation of bulblets, integrating tissue culture with non-tissue culture approaches. Through optimized scale propagation and disinfection treatments, it achieves efficient stable gene overexpression and editing in lily.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3283-3285"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creation of dual-purpose sorghum by CRISPR/Cas9-mediated knockout of Dry gene.","authors":"Fengyong Ge, Wenzhe Zhang, Kangxu Zhao, Baye Wodajo, Ran Xia, Feifei Yu, Qi Xie","doi":"10.1111/jipb.70353","DOIUrl":"10.1111/jipb.70353","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3304-3306"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}