Journal of Integrative Plant Biology最新文献

{"title":"Tradeoff between productivity and stability across above- and below-ground communities","authors":"Zonghao Hu,&nbsp;Haiyan Liu,&nbsp;Junjie Yang,&nbsp;Bin Hua,&nbsp;Michael Bahn,&nbsp;Shuang Pang,&nbsp;Tingting Li,&nbsp;Wei Yang,&nbsp;Honghui Wu,&nbsp;Xingguo Han,&nbsp;Ximei Zhang","doi":"10.1111/jipb.13771","DOIUrl":"10.1111/jipb.13771","url":null,"abstract":"<p>An 11-year nitrogen addition experiment reveals that for both plants and soil microorganisms, the ruderal strategists had higher productivity but lower stability, while the tolerant strategists had higher stability and lower productivity, leading to the tradeoff between productivity and stability within and across above- and below-ground communities.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2321-2324"},"PeriodicalIF":9.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13771","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The METHYLTRANSFERASE B–SERRATE interaction mediates the reciprocal regulation of microRNA biogenesis and RNA m6A modification","authors":"Haiyan Bai,&nbsp;Yanghuan Dai,&nbsp;Panting Fan,&nbsp;Yiming Zhou,&nbsp;Xiangying Wang,&nbsp;Jingjing Chen,&nbsp;Yuzhe Jiao,&nbsp;Chang Du,&nbsp;Zhuoxi Huang,&nbsp;Yuting Xie,&nbsp;Xiaoyu Guo,&nbsp;Xiaoqiang Lang,&nbsp;Yongqing Ling,&nbsp;Yizhen Deng,&nbsp;Qi Liu,&nbsp;Shengbo He,&nbsp;Zhonghui Zhang","doi":"10.1111/jipb.13770","DOIUrl":"10.1111/jipb.13770","url":null,"abstract":"<p>In eukaryotes, RNA <i>N</i>⁶-methyladenosine (m⁶A) modification and microRNA (miRNA)-mediated RNA silencing represent two critical epigenetic regulatory mechanisms. The m⁶A methyltransferase complex (MTC) and the microprocessor complex both undergo liquid–liquid phase separation to form nuclear membraneless organelles. Although m⁶A methyltransferase has been shown to positively regulate miRNA biogenesis, a mechanism of reciprocal regulation between the MTC and the microprocessor complex has remained elusive. Here, we demonstrate that the MTC and the microprocessor complex associate with each other through the METHYLTRANSFERASE B (MTB)–SERRATE (SE) interacting module. Knockdown of <i>MTB</i> impaired miRNA biogenesis by diminishing microprocessor complex binding to primary miRNAs (pri-miRNAs) and their respective <i>MIRNA</i> loci. Additionally, loss of SE function led to disruptions in transcriptome-wide m⁶A modification. Further biochemical assays and fluorescence recovery after photobleaching (FRAP) assay indicated that SE enhances the liquid–liquid phase separation and solubility of the MTC. Moreover, the MTC exhibited enhanced retention on chromatin and diminished binding to its RNA substrates in the <i>se</i> mutant background. Collectively, our results reveal the substantial regulatory interplay between RNA m⁶A modification and miRNA biogenesis.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2613-2631"},"PeriodicalIF":9.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of cryptochrome-mediated blue light signaling by the ABI4–PIF4 module","authors":"Pengyu Song,&nbsp;Zidan Yang,&nbsp;Huaichang Wang,&nbsp;Fangfang Wan,&nbsp;Dingming Kang,&nbsp;Wenming Zheng,&nbsp;Zhizhong Gong,&nbsp;Jigang Li","doi":"10.1111/jipb.13769","DOIUrl":"10.1111/jipb.13769","url":null,"abstract":"<div>\u0000 \u0000 <p>ABSCISIC ACID-INSENSITIVE 4 (ABI4) is a pivotal transcription factor which coordinates multiple aspects of plant growth and development as well as plant responses to environmental stresses. ABI4 has been shown to be involved in regulating seedling photomorphogenesis; however, the underlying mechanism remains elusive. Here, we show that the role of ABI4 in regulating photomorphogenesis is generally regulated by sucrose, but ABI4 promotes hypocotyl elongation of Arabidopsis seedlings under blue (B) light under all tested sucrose concentrations. We further show that ABI4 physically interacts with PHYTOCHROME INTERACTING FACTOR 4 (PIF4), a well-characterized growth-promoting transcription factor, and post-translationally promotes PIF4 protein accumulation under B light. Further analyses indicate that ABI4 directly interacts with the B light photoreceptors cryptochromes (CRYs) and inhibits the interactions between CRYs and PIF4, thus relieving CRY-mediated repression of PIF4 protein accumulation. In addition, while ABI4 could directly activate its own expression, CRYs enhance, whereas PIF4 inhibits, ABI4-mediated activation of the <i>ABI4</i> promoter. Together, our study demonstrates that the ABI4–PIF4 module plays an important role in mediating CRY-induced B light signaling in Arabidopsis.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2412-2430"},"PeriodicalIF":9.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of humidity-sensitive genic male sterility in maize and wheat for hybrid seed production","authors":"Xingchen Xiong,&nbsp;Dan Wang,&nbsp;Changfeng Guo,&nbsp;Guiqiang Fan,&nbsp;Yingchun Zhang,&nbsp;Bo Song,&nbsp;Bingzhu Hou,&nbsp;Yuanyuan Yan,&nbsp;Chuanxiao Xie,&nbsp;Xiaoduo Lu,&nbsp;Chunyi Zhang,&nbsp;Xiaoquan Qi","doi":"10.1111/jipb.13768","DOIUrl":"10.1111/jipb.13768","url":null,"abstract":"<p>Loss of function of a conserved POACEATAPETOL SYNTHASE1 confers humidity sensitive genic male sterility in maize and wheat. This system yielded &gt;99% pure hybrid seed in maize.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2317-2320"},"PeriodicalIF":9.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13768","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A PpEIL2/3–PpNAC1–PpWRKY14 module regulates fruit ripening by modulating ethylene production in peach","authors":"Yudi Liu,&nbsp;Wen Xiao,&nbsp;Liao Liao,&nbsp;Beibei Zheng,&nbsp;Yunpeng Cao,&nbsp;Yun Zhao,&nbsp;Ruo-Xi Zhang,&nbsp;Yuepeng Han","doi":"10.1111/jipb.13761","DOIUrl":"10.1111/jipb.13761","url":null,"abstract":"<div>\u0000 \u0000 <p>WRKY transcription factors play key roles in plant resistance to various stresses, but their roles in fruit ripening remain largely unknown. Here, we report a WRKY gene <i>PpWRKY14</i> involved in the regulation of fruit ripening in peach. The expression of <i>PpWRKY14</i> showed an increasing trend throughout fruit development. <i>PpWRKY14</i> was a target gene of PpNAC1, a master regulator of peach fruit ripening. PpWRKY14 could directly bind to the promoters of <i>PpACS1</i> and <i>PpACO1</i> to induce their expression, and this induction was greatly enhanced when PpWRKY14 formed a dimer with PpNAC1. However, the transcription of <i>PpNAC1</i> could be directly suppressed by two EIN3/EIL1 genes, <i>PpEIL2</i> and <i>PpEIL3</i>. The <i>PpEIL2/3</i> genes were highly expressed at the early stages of fruit development, but their expression was programmed to decrease significantly during the ripening stage, thus derepressing the expression of <i>PpNAC1</i>. These results suggested a PpEIL2/3–PpNAC1–PpWRKY14 module that regulates fruit ripening by modulating ethylene production in peach. Our results provided an insight into the regulatory roles of EIN3/EIL1 and WRKY genes in fruit ripening.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2470-2489"},"PeriodicalIF":9.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anchorene, a carotenoid-derived growth regulator, modulates auxin homeostasis by suppressing GH3-mediated auxin conjugation","authors":"Danping Ke,&nbsp;Yinpeng Xie,&nbsp;Haipeng Li,&nbsp;Liqun Hu,&nbsp;Yi He,&nbsp;Chao Guo,&nbsp;Yahui Zhai,&nbsp;Jinggong Guo,&nbsp;Kun Li,&nbsp;Zongyan Chu,&nbsp;Junli Zhang,&nbsp;Xuebin Zhang,&nbsp;Salim Al-Babili,&nbsp;Kai Jiang,&nbsp;Yuchen Miao,&nbsp;Kun-Peng Jia","doi":"10.1111/jipb.13764","DOIUrl":"10.1111/jipb.13764","url":null,"abstract":"<p>Anchorene, identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid, affects root development by modulating auxin homeostasis. However, the precise interaction between anchorene and auxin, as well as the mechanisms by which anchorene modulates auxin levels, remain largely elusive. In this study, we conducted a comparative analysis of anchorene's bioactivities alongside auxin and observed that anchorene induces multifaceted auxin-like effects. Through genetic and pharmacological examinations, we revealed that anchorene's auxin-like activities depend on the indole-3-pyruvate-dependent auxin biosynthesis pathway, as well as the auxin inactivation pathway mediated by Group II Gretchen Hagen 3 (GH3) proteins that mainly facilitate the conjugation of indole-3-acetic acid (IAA) to amino acids, leading to the formation of inactivated storage forms. Our measurements indicated that anchorene treatment elevates IAA levels while reducing the quantities of inactivated IAA–amino acid conjugates and oxIAA. RNA sequencing further revealed that anchorene triggers the expression of numerous auxin-responsive genes in a manner reliant on Group II GH3s. Additionally, our <i>in vitro</i> enzymatic assays and biolayer interferometry (BLI) assay demonstrated anchorene's robust suppression of GH3.17-mediated IAA conjugation with glutamate. Collectively, our findings highlight the significant role of carotenoid-derived metabolite anchorene in modulating auxin homeostasis, primarily through the repression of GH3-mediated IAA conjugation and inactivation pathways, offering novel insights into the regulatory mechanisms of plant bioactive apocarotenoids.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2490-2504"},"PeriodicalIF":9.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome of Eleocharis vivipara elucidates the genetics of C3–C4 photosynthetic plasticity and karyotype evolution in the Cyperaceae","authors":"Hongbing Liu,&nbsp;Hang Zhao,&nbsp;Yanwen Zhang,&nbsp;Xiuli Li,&nbsp;Yi Zuo,&nbsp;Zhen Wu,&nbsp;Kaining Jin,&nbsp;Wenfei Xian,&nbsp;Wenzheng Wang,&nbsp;Weidong Ning,&nbsp;Zijian Liu,&nbsp;Xiaoxiao Zhao,&nbsp;Lei Wang,&nbsp;Rowan F. Sage,&nbsp;Tiegang Lu,&nbsp;Matt Stata,&nbsp;Shifeng Cheng","doi":"10.1111/jipb.13765","DOIUrl":"10.1111/jipb.13765","url":null,"abstract":"<p><i>Eleocharis vivipara</i>, an amphibious sedge in the Cyperaceae family, has several remarkable properties, most notably its alternate use of C₃ photosynthesis underwater and C₄ photosynthesis on land. However, the absence of genomic data has hindered its utility for evolutionary and genetic research. Here, we present a high-quality genome for <i>E. vivipara</i>, representing the first chromosome-level genome for the <i>Eleocharis</i> genus, with an approximate size of 965.22 Mb mainly distributed across 10 chromosomes. Its Hi–C pattern, chromosome clustering results, and one-to-one genome synteny across two subgroups indicates a tetraploid structure with chromosome count 2<i>n</i> = 4<i>x</i> = 20. Phylogenetic analysis suggests that <i>E. vivipara</i> diverged from <i>Cyperus esculentus</i> approximately 32.96 million years ago (Mya), and underwent a whole-genome duplication (WGD) about 3.5 Mya. Numerous fusion and fission events were identified between the chromosomes of <i>E. vivipara</i> and its close relatives. We demonstrate that <i>E. vivipara</i> has holocentromeres, a chromosomal feature which can maintain the stability of such chromosomal rearrangements. Experimental transplantation and cross-section studies showed its terrestrial culms developed C₄ Kranz anatomy with increased number of chloroplasts in the bundle sheath (BS) cells. Gene expression and weighted gene co-expression network analysis (WGCNA) showed overall elevated expression of core genes associated with the C₄ pathway, and significant enrichment of genes related to modified culm anatomy and photosynthesis efficiency. We found evidence of mixed nicotinamide adenine dinucleotide - malic enzyme and phosphoenolpyruvate carboxykinase type C₄ photosynthesis in <i>E. vivipara</i>, and hypothesize that the evolution of C₄ photosynthesis predates the WGD event. The mixed type is dominated by subgenome A and supplemented by subgenome B. Collectively, our findings not only shed light on the evolution of <i>E. vivipara</i> and karyotype within the Cyperaceae family, but also provide valuable insights into the transition between C₃ and C₄ photosynthesis, offering promising avenues for crop improvement and breeding.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2505-2527"},"PeriodicalIF":9.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13765","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The OsAGO2–OsNAC300–OsNAP module regulates leaf senescence in rice","authors":"Shaoyan Zheng,&nbsp;Junyu Chen,&nbsp;Ying He,&nbsp;Jingqin Lu,&nbsp;Hong Chen,&nbsp;Zipeng Liang,&nbsp;Junqi Zhang,&nbsp;Zhenlan Liu,&nbsp;Jing Li,&nbsp;Chuxiong Zhuang","doi":"10.1111/jipb.13766","DOIUrl":"10.1111/jipb.13766","url":null,"abstract":"<p>Leaves play a crucial role in the growth and development of rice (<i>Oryza sativa</i>) as sites for the production of photosynthesis. Early leaf senescence leads to substantial drops in rice yields. Whether and how DNA methylation regulates gene expression and affects leaf senescence remains elusive. Here, we demonstrate that mutations in rice <i>ARGONAUTE 2</i> (<i>OsAGO2</i>) lead to premature leaf senescence, with chloroplasts in <i>Osago2</i> having lower chlorophyll content and an abnormal thylakoid structure compared with those from wild-type plants. We show that OsAGO2 associates with a 24-nt microRNA and binds to the promoter region of <i>OsNAC300</i>, which causes DNA methylation and suppressed expression of <i>OsNAC300</i>. Overexpressing <i>OsNAC300</i> causes the similar premature leaf senescence as <i>Osago2</i> mutants and knocking out <i>OsNAC300</i> in the <i>Osago2</i> mutant background suppresses the early senescence of <i>Osago2</i> mutants. Based on yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays, we propose that OsNAC300 directly regulates transcription of the key rice aging gene NAC-like, activated by APETALA3/PISTILLATA (<i>OsNAP</i>) to control leaf senescence. Our results unravel a previously unknown epigenetic regulatory mechanism underlying leaf senescence in which OsAGO2–OsNAC300<i>–OsNAP</i> acts as a key regulatory module of leaf senescence to maintain leaf function.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2395-2411"},"PeriodicalIF":9.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142015743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The plant terpenes DMNT and TMTT function as signaling compounds that attract Asian corn borer (Ostrinia furnacalis) to maize plants","authors":"Mengjie Zhao,&nbsp;Shijie Huang,&nbsp;Qingyang Zhang,&nbsp;Yuming Wei,&nbsp;Zhen Tao,&nbsp;Chuanhong Wang,&nbsp;Yibing Zhao,&nbsp;Xinqiao Zhang,&nbsp;Jinghui Dong,&nbsp;Ling Wang,&nbsp;Chen Chen,&nbsp;Tengyue Wang,&nbsp;Peijin Li","doi":"10.1111/jipb.13763","DOIUrl":"10.1111/jipb.13763","url":null,"abstract":"<p>During their co-evolution with herbivorous insects, plants have developed multiple defense strategies that resist pests, such as releasing a blend of herbivory-induced plant volatiles (HIPVs) that repel pests or recruit their natural enemies. However, the responses of insects to HIPVs in maize (<i>Zea mays</i> L.) are not well understood. Here, we demonstrate that the Asian corn borer (ACB, <i>Ostrinia furnacalis</i>), a major insect pest of maize, shows a preference for maize pre-infested with ACB larvae rather than being repelled by these plants. Through combined transcriptomic and metabolomics analysis of ACB-infested maize seedlings, we identified two substances that explain this behavior: (<i>E</i>)-4,8-dimethylnona-1,3,7-triene (DMNT) and (3<i>E</i>,7<i>E</i>)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). DMNT and TMTT attracted ACB larvae, and knocking out the maize genes responsible for their biosynthesis via gene editing impaired this attraction. External supplementation with DMNT/TMTT hampered the larvae's ability to locate pre-infested maize. These findings uncover a novel role for DMNT and TMTT in driving the behavior of ACB. Genetic modification of maize to make it less detectable by ACB might be an effective strategy for developing maize germplasm resistant to ACB and for managing this pest effectively in the field.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2528-2542"},"PeriodicalIF":9.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142015744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The origin and morphological character evolution of the paleotropical woody bamboos","authors":"Jing-Xia Liu,&nbsp;Cen Guo,&nbsp;Peng-Fei Ma,&nbsp;Meng-Yuan Zhou,&nbsp;Ya-Huang Luo,&nbsp;Guang-Fu Zhu,&nbsp;Zu-Chang Xu,&nbsp;Richard I Milne,&nbsp;Maria S. Vorontsova,&nbsp;De-Zhu Li","doi":"10.1111/jipb.13751","DOIUrl":"10.1111/jipb.13751","url":null,"abstract":"<p>The woody bamboos (Bambusoideae) exhibit distinctive biological traits within Poaceae, such as highly lignified culms, rapid shoot growth, monocarpic mass flowering and nutlike or fleshy caryopses. Much of the remarkable morphological diversity across the subfamily exists within a single hexaploid clade, the paleotropical woody bamboos (PWB), making it ideal to investigate the factors underlying morphological evolution in woody bamboos. However, the origin and biogeographical history of PWB remain elusive, as does the effect of environmental factors on the evolution of their morphological characters. We generated a robust and time-calibrated phylogeny of PWB using single nucleotide polymorphisms retrieved from optimized double digest restriction site associated DNA sequencing, and explored the evolutionary trends of habit, inflorescence, and caryopsis type in relation to environmental factors including climate, soil, and topography. We inferred that the PWB started to diversify across the Oligocene–Miocene boundary and formed four major clades, that is, Melocanninae, Racemobambosinae <i>s.l.</i> (comprising Dinochloinae, Greslanlinae, Racemobambosinae <i>s.str.</i> and Temburongiinae), Hickeliinae and Bambusinae <i>s.l.</i> (comprising Bambusinae <i>s.str.</i> plus Holttumochloinae). The ancestor of PWB was reconstructed as having erect habit, indeterminate inflorescence and basic caryopsis. The characters including climbing/scrambling habit, determinate inflorescence, and nucoid/bacoid caryopsis have since undergone multiple changes and reversals during the diversification of PWB. The evolution of all three traits was correlated with, and hence likely influenced by, aspects of climate, topography, and soil, with climate factors most strongly correlated with morphological traits, and soil factors least so. However, topography had more influence than climate or soil on the evolution of erect habit, whereas both factors had greater effect on the evolution of bacoid caryopsis than did soil. Our results provide novel insights into morphological diversity and adaptive evolution in bamboos for future ecological and evolutionary research.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2242-2261"},"PeriodicalIF":9.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142015742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}