Journal of Integrative Plant Biology最新文献

{"title":"Rice black-streaked dwarf virus-encoded P6 protein impairs OsPelota-mediated antiviral RNA decay defense via promoting OsSCE1b ubiquitination and degradation in rice","authors":"Yi Xie,&nbsp;Ming Zeng,&nbsp;Dan Wang,&nbsp;Shi-bo Gao,&nbsp;Liyan Li,&nbsp;Lianshun Zheng,&nbsp;Yunge Zhang,&nbsp;Shifang Fei,&nbsp;Cui Zhang,&nbsp;Yaqin Wang,&nbsp;Xueping Zhou,&nbsp;Jianxiang Wu","doi":"10.1111/jipb.13966","DOIUrl":"10.1111/jipb.13966","url":null,"abstract":"<p>Rice black-streaked dwarf virus (RBSDV) is a major viral pathogen threatening rice production worldwide. However, the molecular mechanisms underlying the arms race between RBSDV and its host remain largely elusive. Here, we demonstrate that RBSDV infection, or the expression of viral RNA-silencing suppressor protein P6, promotes the ubiquitination and degradation of rice small ubiquitin-like modifiers (SUMO) conjugating enzyme 1b (OsSCE1b). OsSCE1b catalyzes the SUMOylation of OsPelota, a protein involved in plant antiviral RNA decay. Furthermore, RBSDV P6 enhances the interaction between rice ubiquitin E3 ligases SINAT3/4/5 and OsSCE1b in the cytoplasm, leading to increased ubiquitination and degradation of OsSCE1b. Rice plants overexpressing OsSCE1b exhibited reduced susceptibility to RBSDV infection. Conversely, OsSCE1b knockdown and knockout lines, as well as OsPelota knockout lines, were more susceptible, indicating that both OsSCE1b and OsPelota negatively regulate RBSDV infection. Additionally, our findings show that OsSCE1b-catalyzed SUMOylated OsPelota interacts with the Hsp70 subfamily B suppressor OsHBS1, forming a complex that degrades RBSDV genomic RNAs containing one or more GA₆ motifs. Taken together, our data demonstrate that OsSCE1b negatively regulates RBSDV infection by promoting OsPelota SUMOylation and activating the antiviral RNA decay activity of the OsPelota–OsHBS1 complex. Conversely, RBSDV P6 promotes viral infection by enhancing OsSCE1b ubiquitination and degradation, thereby suppressing OsPelota SUMOylation and the rice antiviral RNA decay defense response.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2760-2777"},"PeriodicalIF":9.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13966","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The horizontally transferred gene, CsMTAN, rewired purine traffic to build caffeine factories in tea leaves.","authors":"Xinxin Jia, Xiaoliang Zhang, Xueli Chen, Alisdair R Fernie, Weiwei Wen","doi":"10.1111/jipb.13957","DOIUrl":"https://doi.org/10.1111/jipb.13957","url":null,"abstract":"<p><p>Purine-related metabolites are central to primary metabolic pathways in plants and serve as precursors for purine alkaloid biosynthesis in caffeinated species such as tea plants (Camellia sinensis). In this study, metabolite profiling of two tissues (young and mature leaves) was performed across 183 genetically diverse tea accessions, identifying and quantifying 10 purine alkaloid-related metabolites. Metabolite genome-wide association studies revealed 17 significant loci associated with these metabolites, including both known loci such as caffeine synthase and 16 novel loci (P < 1.05 × 10^-5). Through functional annotation and in vitro enzymatic assay, we characterized 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (CsMTAN) as the causal gene underlying natural variation in adenosine and adenine content. CsMTAN can catalyze the degradation of both 5'-methylthioadenosine and S-adenosylhomocysteine to release adenine. The T → A nucleotide substitution at SNP55151898, which leads to a phenylalanine → tyrosine substitution at residue 179 (F179Y), resulted in a significant alteration of enzyme activity in vitro, as evidenced by an approximately 50% reduction in adenine abundance (P < 0.05). Transient overexpression of CsMTAN-A and CsMTAN-T in Nicotiana benthamiana both significantly increased adenine content and dramatically decreased adenosine content, providing direct evidence for the functional involvement of CsMTAN in plant purine metabolism. CsMTAN-T overexpression resulted in significantly lower adenosine level than CsMTAN-A (P < 0.05). Phylogenetic analysis across 115 species and protein structural modeling revealed a distinct evolutionary divergence between plant MTAN evolution and species phylogeny, strongly suggesting the occurrence of horizontal gene transfer events in the evolutionary history of plant MTANs. This study thus furthered our understanding of the genetics and molecular mechanisms regulating purine metabolism and purine alkaloid biosynthesis in tea plants and provided novel targets for molecular breeding and synthetic biology applications.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13697","DOIUrl":"https://doi.org/10.1111/jipb.13697","url":null,"abstract":"<p>The cover illustrates serotonin's role in safeguarding plants from stress-induced damage. In the classic Chinese novel “Journey to the West”, the Monkey King embodies divine power, mirroring the role of serotonin in plant stress responses. The cover illustration depicts serotonin as the Monkey King, battling plant stress, represented by the White Bone Demon, to protect the plant. Sun <i>et al.</i> (pages 1706–1724) provide a comprehensive review of the multifaceted roles of serotonin in plant stress responses, highlighting its potential as a key regulator in enhancing plant resilience.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 7","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GIGANTEA-LATE ELONGATED HYPOCOTYL complex regulates citrus drought tolerance and drought induced flowering","authors":"Tian-Liang Zhang,&nbsp;Min Chen,&nbsp;Yong-Huan Wan,&nbsp;Jian-Yun Qiu,&nbsp;Yong-Zhen Wen,&nbsp;Zhi-Meng Gan,&nbsp;Zhong-Xiang Ma,&nbsp;Wen-Feng Wang,&nbsp;Jing-Jing Zhou,&nbsp;Yu-Xia Du,&nbsp;Chun-Gen Hu,&nbsp;Jin-Zhi Zhang","doi":"10.1111/jipb.13956","DOIUrl":"10.1111/jipb.13956","url":null,"abstract":"<div>\u0000 \u0000 <p>Drought severely impedes plant growth and production as a primary abiotic stress. <i>GIGANTEA</i> (<i>GI</i>) regulates flowering and responds to various stresses in model plants; however, its function remains poorly understood in non-model plants. In this study, a <i>Citrus limon GI</i> homologous (<i>CiGI</i>) was identified and two alternative splicing transcripts (<i>CiGIα</i> and <i>CiGIβ</i>) were found. <i>CiGIα</i> overexpressing tobacco exhibited early flowering and drought sensitivity, whereas the phenotype of <i>CiGIβ-</i>overexpressing plants was similar to that of wild-type (WT) plants. Overexpression of <i>CiGIα</i> in citrus increased drought sensitivity and upregulated <i>citrus FLOWERING LOCUS T (CiFT)</i> expression, and downregulation of <i>CiGI</i> enhanced drought tolerance. Further studies revealed that CiGIα, CiGIβ, and LATE ELONGATED HYPOCOTYL (CiLHY) form a complex that binds to the <i>Nuclear Factor YA1</i> (<i>CiNF-YA1</i>) promoter and activates its expression. Subsequently, CiNF-YA1 activates the expression of <i>NADP-DEPENDENT MALIC ENZYME 2</i> (<i>CiNADP-ME2</i>) by binding its promoter, leading to increased reactive oxygen species (ROS) accumulation, which enhances plant drought sensitivity. Exogenous ROS treatment induced citrus flowering and reduced drought tolerance. Furthermore, the CiGI–CiLHY complex also activates <i>CiFT</i> and may participate in the regulation of citrus flowering. These results reveal a novel mechanism by which <i>CiGI</i> regulates citrus flowering and drought tolerance.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2366-2387"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato TGase positively regulates thermotolerance by inducing polyamine to activate autophagy","authors":"Min Zhong,&nbsp;Qingshen Cui,&nbsp;Yan Yang,&nbsp;Ke Zhang,&nbsp;Xiaoying Liu,&nbsp;Guan Pang,&nbsp;Lifei Yang,&nbsp;Shirong Guo,&nbsp;Jin Sun,&nbsp;Yu Wang","doi":"10.1111/jipb.13955","DOIUrl":"10.1111/jipb.13955","url":null,"abstract":"<div>\u0000 \u0000 <p>Transglutaminases (TGases) are multifunctional enzymes involved in stress responses, while autophagy is a key cellular degradation process. However, the relationship between TGases and autophagy in the plant heat stress response remains poorly understood. In this study, we demonstrated that TGase was essential for heat tolerance by regulating autophagy. Heat stress induced both <i>TGase</i> expression and activity. The <i>tgase</i> mutants reduced, while <i>TGase</i>-overexpression (<i>TGase</i>OE) lines increased plant thermotolerance. Under heat stress, insoluble proteins were more ubiquitinated in <i>tgase</i> mutants and less so in <i>TGase</i>OE plants. Moreover, TGase promoted the expression of autophagy-related (<i>ATG</i>) genes and autophagosome formation. Polyamine content and the expression of polyamine-related genes, particularly <i>SAMS2</i>, were positively correlated with TGase activity. TGase interacted with SAMS2 both <i>in vitro</i> and <i>in vivo</i>, and knockout of <i>SAMS2</i> impaired TGase-induced thermotolerance and autophagosome formation in <i>TGase</i>OE plants. Exogenous spermidine also promoted autophagosome formation in <i>tgase</i> mutants, indicating a critical role of polyamine in TGase-mediated heat tolerance and autophagosome formation. Furthermore, a cell-free degradation assay showed that TGase enhanced the stability of SAMS2. Altogether, these results reveal that TGase interacts with and stabilizes SAMS2 to promote polyamine synthesis, which upregulates <i>ATG</i> gene expression and facilitates autophagosome formation to degrade ubiquitinated proteins, thereby enhancing the thermotolerance of tomato plants.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2350-2365"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13696","DOIUrl":"https://doi.org/10.1111/jipb.13696","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 7","pages":"1687-1688"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variation for adaptive evolution in response to changed environments in plants","authors":"Jing Hou,&nbsp;Meng Liu,&nbsp;Kai Yang,&nbsp;Bao Liu,&nbsp;Huanhuan Liu,&nbsp;Jianquan Liu","doi":"10.1111/jipb.13961","DOIUrl":"10.1111/jipb.13961","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants adapt to their local environments through natural or artificial selection of optimal phenotypes. Recent advances in genomics and computational biology, which integrate phenotypic and multi-omics data, have facilitated the rapid identification of key genes and allelic variations that underlie these adaptive evolutionary processes. Understanding the underlying molecular mechanisms has significantly enhanced our knowledge of how plants respond to changed habitats, including various biotic and abiotic stresses. In this review, we highlight recent progress in elucidating the genetic basis of phenotypic variation in morphological traits and stress responses, as well as the emergence of new ecotypes, subspecies, and species during adaptive evolution across varied environments. This occurs through allelic divergences in both coding and non-coding regions in both model and non-model plants. Furthermore, the terrestrialization and early diversification of land plants involved the acquisition of additional genes, primarily through horizontal gene transfer and whole-genome duplication, which facilitated the development of complex molecular pathways to adapt to increasingly diverse environments. Finally, we discuss emerging trends and prospects for exploring and utilizing beneficial alleles for environmental adaptation, to guide crop breeding efforts in response to global climate change.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2265-2293"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant viral effector disrupts ALD1-OSB1 immunity module to suppress chloroplast defenses","authors":"Zuxian Pan,&nbsp;Yaqin Wang,&nbsp;Fangfang Li,&nbsp;Yuzhen Mei,&nbsp;Xueping Zhou","doi":"10.1111/jipb.13959","DOIUrl":"10.1111/jipb.13959","url":null,"abstract":"<div>\u0000 \u0000 <p>Chloroplasts are central to plant immunity, with the chloroplast-localized protein AGD2-LIKE DEFENSE RESPONSE PROTEIN 1 (ALD1) playing a critical role in producing pipecolic acid (Pip), a key immune signal. However, the regulation of ALD1 and how pathogens evade ALD1-mediated defenses remain poorly understood. Using the geminivirus tomato yellow leaf curl China virus and its associated betasatellite (TYLCCNV/TYLCCNB) as a model, we uncovered a defense mechanism involving organellar single-stranded DNA-binding protein 1 (OSB1), which stabilizes ALD1 and promotes Pip biosynthesis to strengthen immunity. Crucially, the viral βC1 effector encoded by TYLCCNB disrupts this pathway by binding OSB1 and sequestering it away from chloroplasts, thereby blocking OSB1–ALD1 interaction, destabilizing ALD1, and suppressing Pip-dependent defenses. Strikingly, βC1 mutants defective in OSB1 binding fail to interfere with the OSB1–ALD1 stability, and TYLCCNV infections carrying these mutants induce attenuated symptoms in <i>Nicotiana benthamiana</i>. Our study not only reveals how ALD1–OSB1 cooperates in chloroplast immunity but also demonstrates how geminiviruses, as a tractable model, can dissect pathogen counter-defense strategies.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2510-2524"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial complex I assembles via a distal-pump module (PD)-early and PD-late pathway in maize","authors":"Baoyin Chen,&nbsp;Manna Huang,&nbsp;Junjun Wang,&nbsp;Yuanye Gui,&nbsp;Qingqing Wei,&nbsp;Zhihui Xiu,&nbsp;Feng Sun,&nbsp;Yan-Zhuo Yang,&nbsp;Chunhui Xu,&nbsp;Bao-Cai Tan","doi":"10.1111/jipb.13958","DOIUrl":"10.1111/jipb.13958","url":null,"abstract":"<div>\u0000 \u0000 <p>The L-shape mitochondrial complex I (CI) consists of four modules: nicotinamide-adenine dinucleotide hydrogen-binding module (N) and ubiquinone-binding module (Q) in the matrix arm and proximal-pump module (P_P) and distal-pump module (P_D) in the membrane arm. As mitochondrial mutants are unavailable, the CI assembly pathway in plants is unclear. We investigated the CI assembly process using the maize RNA processing mutants deficient in individual CI components. Complexome profiling detected all major assembly intermediates of each module, confirming their independent assembly pathway. A block in the Q module assembly causes the accumulation of the membrane arm, whereas a block in the P_D module assembly results in the accumulation of CI*, a subcomplex assembled by P_P with the matrix arm. We further isolated and analyzed the mutants of two CI assembly factors, ZmGLDH and CRK1. The absence of either ZmGLDH or CRK1 eliminates the accumulation of CI* but allows a substantial amount of CI to be assembled. The membrane arm was also accumulated in <i>zmgldh</i> and <i>crk1</i>. Together, these results suggest two CI assembly pathways. The P_D-early former starts with the assembly of P_P with P_D, forming the membrane arm, then joins with the matrix arm to produce CI. The P_D-late pathway is initiated by assembling P_P with the matrix arm to yield CI*, which is then combined with P_D to form CI.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2461-2479"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TaIRE1-mediated unconventional splicing of the TabZIP60 mRNA and the miR172 precursor regulates heat stress tolerance in wheat","authors":"Haoran Li,&nbsp;Zhen Qin,&nbsp;Xiaoli Geng,&nbsp;Jie Cao,&nbsp;Xinyang Yuan,&nbsp;Huiru Peng,&nbsp;Yingyin Yao,&nbsp;Zhaorong Hu,&nbsp;Weilong Guo,&nbsp;Yumei Zhang,&nbsp;Jie Liu,&nbsp;Vincenzo Rossi,&nbsp;Ive De Smet,&nbsp;Zhongfu Ni,&nbsp;Qixin Sun,&nbsp;Mingming Xin","doi":"10.1111/jipb.13963","DOIUrl":"10.1111/jipb.13963","url":null,"abstract":"<p>INOSITOL-REQUIRING ENZYME 1 (IRE1) is conserved in plants and mammals to regulate stress responses. Here, we found that <i>TaIRE1</i> is involved in the unconventional splicing of cell membrane-localized <i>TabZIP60</i> messenger RNA (mRNA), which results in a nucleus resident protein form (TabZIP60s), and enhanced heat stress tolerance. Transcriptome analysis together with binding element prediction revealed 121 high-confidence targets of TabZIP60s responsive to heat stress in wheat (<i>Triticum aestivum</i>), including heat shock protein genes. Interestingly, we found that an asparagine to glutamic acid substitution, located next to DNA-binding domain of TabZIP60s, results in reduced binding affinity and transcriptional activity to downstream targets, and this heat stress tolerance inferior allele was positively selected during modern wheat breeding programs in China, possibly due to their negative effects on yield potential. Finally, we showed that <i>TaIRE1</i> is also responsible for the mis-cleavage of miR172 precursors, and consequently contribute to heat stress tolerance. To the best of our knowledge, this represents the first report showing that, like in mammals, IRE1 also regulates miRNA cleavage in response to heat stress in plants. Together, this coordinate control of two signaling pathways provides new insights into heat stress tolerance regulation in wheat.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2388-2400"},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13963","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}