Journal of Integrative Plant Biology最新文献

{"title":"The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution","authors":"Yuan-Bao Li,&nbsp;Chengyu Liu,&nbsp;Ningning Shen,&nbsp;Shuai Zhu,&nbsp;Xianya Deng,&nbsp;Zixuan Liu,&nbsp;Li-Bo Han,&nbsp;Dingzhong Tang","doi":"10.1111/jipb.13744","DOIUrl":"10.1111/jipb.13744","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Magnaporthe oryzae</i> (<i>M. oryzae</i>) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense-related complexes such as proteins, callose-rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, <i>Myosin A1</i> (<i>OsMYA1</i>), contributed to rice defense. The <i>OsMYA1</i> knockout mutant exhibited decreased resistance to <i>M. oryzae</i> infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of <i>M. oryzae</i>. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the <i>OsMYA1</i> and <i>OsExo70H1</i> mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against <i>M. oryzae</i> by OsMYA1-initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"2058-2075"},"PeriodicalIF":9.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13744","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous mutations in ITPK4 and MRP5 genes result in a low phytic acid level without compromising salt tolerance in Arabidopsis","authors":"Yuying Ren,&nbsp;Mengdan Jiang,&nbsp;Jian-Kang Zhu,&nbsp;Wenkun Zhou,&nbsp;Chunzhao Zhao","doi":"10.1111/jipb.13745","DOIUrl":"10.1111/jipb.13745","url":null,"abstract":"<div>\u0000 \u0000 <p>Generation of crops with low phytic acid (<i>myo</i>-inositol-1,2,3,4,5,6-hexakisphosphate (InsP₆)) is an important breeding direction, but such plants often display less desirable agronomic traits. In this study, through ethyl methanesulfonate-mediated mutagenesis, we found that inositol 1,3,4-trisphosphate 5/6-kinase 4 (ITPK4), which is essential for producing InsP₆, is a critical regulator of salt tolerance in <i>Arabidopsis</i>. Loss of function of <i>ITPK4</i> gene leads to reduced root elongation under salt stress, which is primarily because of decreased root meristem length and reduced meristematic cell number. The <i>itpk4</i> mutation also results in increased root hair density and increased accumulation of reactive oxygen species during salt exposure. RNA sequencing assay reveals that several auxin-responsive genes are down-regulated in the <i>itpk4-1</i> mutant compared to the wild-type. Consistently, the <i>itpk4-1</i> mutant exhibits a reduced auxin level in the root tip and displays compromised gravity response, indicating that ITPK4 is involved in the regulation of the auxin signaling pathway. Through suppressor screening, it was found that mutation of <i>Multidrug Resistance Protein 5</i> (<i>MRP5</i>)<i>5</i> gene, which encodes an ATP-binding cassette (ABC) transporter required for transporting InsP₆ from the cytoplasm into the vacuole, fully rescues the salt hypersensitivity of the <i>itpk4-1</i> mutant, but in the <i>itpk4-1 mrp5</i> double mutant, InsP₆ remains at a very low level. These results imply that InsP₆ homeostasis rather than its overall amount is beneficial for stress tolerance in plants. Collectively, this study uncovers a pair of gene mutations that confer low InsP₆ content without impacting stress tolerance, which offers a new strategy for creating “low-phytate” crops.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2109-2125"},"PeriodicalIF":9.3,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A","authors":"Miqi Xu,&nbsp;Yi-Yuan Wang,&nbsp;Yujie Wu,&nbsp;Xiuhong Zhou,&nbsp;Ziyan Shan,&nbsp;Kunying Tao,&nbsp;Kaiqiang Qian,&nbsp;Xuncheng Wang,&nbsp;Jian Li,&nbsp;Qingqing Wu,&nbsp;Xing Wang Deng,&nbsp;Jun-Jie Ling","doi":"10.1111/jipb.13742","DOIUrl":"10.1111/jipb.13742","url":null,"abstract":"<div>\u0000 \u0000 <p>Although green light (GL) is located in the middle of the visible light spectrum and regulates a series of plant developmental processes, the mechanism by which it regulates seedling development is largely unknown. In this study, we demonstrated that GL promotes atypical photomorphogenesis in <i>Arabidopsis thaliana</i> via the dual regulations of phytochrome B (phyB) and phyA. Although the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light (RL) in a fluence rate-dependent and time-dependent manner, long-term treatment with GL induced high Pfr/Pr ratios of phyB and phyA. Moreover, GL induced the formation of numerous small phyB photobodies in the nucleus, resulting in atypical photomorphogenesis, with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to RL. The abundance of phyA significantly decreased after short- and long-term GL treatments. We determined that four major PHYTOCHROME-INTERACTING FACTORs (PIFs: PIF1, PIF3, PIF4, and PIF5) act downstream of phyB in GL-mediated cotyledon opening. In addition, GL plays opposite roles in regulating different PIFs. For example, under continuous GL, the protein levels of all PIFs decreased, whereas the transcript levels of <i>PIF4</i> and <i>PIF5</i> strongly increased compared with dark treatment. Taken together, our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1915-1933"},"PeriodicalIF":9.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ripening and rot: How ripening processes influence disease susceptibility in fleshy fruits","authors":"Shan Li,&nbsp;Yu Zhao,&nbsp;Pan Wu,&nbsp;Donald Grierson,&nbsp;Lei Gao","doi":"10.1111/jipb.13739","DOIUrl":"10.1111/jipb.13739","url":null,"abstract":"<p>Fleshy fruits become more susceptible to pathogen infection when they ripen; for example, changes in cell wall properties related to softening make it easier for pathogens to infect fruits. The need for high-quality fruit has driven extensive research on improving pathogen resistance in important fruit crops such as tomato (<i>Solanum lycopersicum</i>). In this review, we summarize current progress in understanding how changes in fruit properties during ripening affect infection by pathogens. These changes affect physical barriers that limit pathogen entry, such as the fruit epidermis and its cuticle, along with other defenses that limit pathogen growth, such as preformed and induced defense compounds. The plant immune system also protects ripening fruit by recognizing pathogens and initiating defense responses involving reactive oxygen species production, mitogen-activated protein kinase signaling cascades, and jasmonic acid, salicylic acid, ethylene, and abscisic acid signaling. These phytohormones regulate an intricate web of transcription factors (TFs) that activate resistance mechanisms, including the expression of pathogenesis-related genes. In tomato, ripening regulators, such as RIPENING INHIBITOR and NON_RIPENING, not only regulate ripening but also influence fruit defenses against pathogens. Moreover, members of the ETHYLENE RESPONSE FACTOR (ERF) family play pivotal and distinct roles in ripening and defense, with different members being regulated by different phytohormones. We also discuss the interaction of ripening-related and defense-related TFs with the Mediator transcription complex. As the ripening processes in climacteric and non-climacteric fruits share many similarities, these processes have broad applications across fruiting crops. Further research on the individual contributions of ERFs and other TFs will inform efforts to diminish disease susceptibility in ripe fruit, satisfy the growing demand for high-quality fruit and decrease food waste and related economic losses.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1831-1863"},"PeriodicalIF":9.3,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sphingolipid inhibitor response gene GhMYB86 controls fiber elongation by regulating microtubule arrangement","authors":"Fan Xu,&nbsp;Guiming Li,&nbsp;Shengyang He,&nbsp;Zhifeng Zeng,&nbsp;Qiaoling Wang,&nbsp;Hongju Zhang,&nbsp;Xingying Yan,&nbsp;Yulin Hu,&nbsp;Huidan Tian,&nbsp;Ming Luo","doi":"10.1111/jipb.13740","DOIUrl":"10.1111/jipb.13740","url":null,"abstract":"<p>Although the cell membrane and cytoskeleton play essential roles in cellular morphogenesis, the interaction between the membrane and cytoskeleton is poorly understood. Cotton fibers are extremely elongated single cells, which makes them an ideal model for studying cell development. Here, we used the sphingolipid biosynthesis inhibitor, fumonisin B1 (FB1), and found that it effectively suppressed the myeloblastosis (MYB) transcription factor GhMYB86, thereby negatively affecting fiber elongation. A direct target of GhMYB86 is <i>GhTUB7</i>, which encodes the tubulin protein, the major component of the microtubule cytoskeleton. Interestingly, both the overexpression of <i>GhMYB86</i> and <i>GhTUB7</i> caused an ectopic microtubule arrangement at the fiber tips, and then leading to shortened fibers. Moreover, we found that GhMBE2 interacted with GhMYB86 and that FB1 and reactive oxygen species induced its transport into the nucleus, thereby enhancing the promotion of <i>GhTUB7</i> by GhMYB86. Overall, we established a GhMBE2-GhMYB86-<i>GhTUB7</i> regulation module for fiber elongation and revealed that membrane sphingolipids affect fiber elongation by altering microtubule arrangement.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1898-1914"},"PeriodicalIF":9.3,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic variation of 363 diverse tea accessions unveils the genetic diversity, domestication, and structural variations associated with tea adaptation","authors":"Wei Tong,&nbsp;Yanli Wang,&nbsp;Fangdong Li,&nbsp;Fei Zhai,&nbsp;Jingjing Su,&nbsp;Didi Wu,&nbsp;Lianghui Yi,&nbsp;Qijuan Gao,&nbsp;Qiong Wu,&nbsp;Enhua Xia","doi":"10.1111/jipb.13737","DOIUrl":"10.1111/jipb.13737","url":null,"abstract":"<div>\u0000 \u0000 <p>Domestication has shaped the population structure and agronomic traits of tea plants, yet the complexity of tea population structure and genetic variation that determines these traits remains unclear. We here investigated the resequencing data of 363 diverse tea accessions collected extensively from almost all tea distributions and found that the population structure of tea plants was divided into eight subgroups, which were basically consistent with their geographical distributions. The genetic diversity of tea plants in China decreased from southwest to east as latitude increased. Results also indicated that <i>Camellia sinensis</i> var. <i>assamica</i> (CSA) illustrated divergent selection signatures with <i>Camellia sinensis</i> var. <i>sinensis</i> (CSS). The domesticated genes of CSA were mainly involved in leaf development, flavonoid and alkaloid biosynthesis, while the domesticated genes in CSS mainly participated in amino acid metabolism, aroma compounds biosynthesis, and cold stress. Comparative population genomics further identified ~730 Mb novel sequences, generating 6,058 full-length protein-encoding genes, significantly expanding the gene pool of tea plants. We also discovered 217,376 large-scale structural variations and 56,583 presence and absence variations (PAVs) across diverse tea accessions, some of which were associated with tea quality and stress resistance. Functional experiments demonstrated that two PAV genes (<i>CSS0049975</i> and <i>CSS0006599</i>) were likely to drive trait diversification in cold tolerance between CSA and CSS tea plants. The overall findings not only revealed the genetic diversity and domestication of tea plants, but also underscored the vital role of structural variations in the diversification of tea plant traits.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2175-2190"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13523","DOIUrl":"https://doi.org/10.1111/jipb.13523","url":null,"abstract":"<p>Grapes, first written about in The Book of Songs, have been cultivated for millennia and have become globally valued as a fresh fruit and for making wine. The cover depicts grapevines growing along the coast in Shenzhen, China, where they produce a bountiful harvest brought by the gentle breeze. However, these grapevines are not delicate—they can withstand the sea wind and the splashing of seawater, symbolizing the tenacious vitality of grapes that are adapted to the coastal environment. The population genomics study by Zhang et al. (pages 1408–1426) offers valuable insights for breeding salt-tolerant grape rootstocks and identifies key structural variations, advancing horticulture of this economically and culturally significant crop.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 7","pages":"C1"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13522","DOIUrl":"https://doi.org/10.1111/jipb.13522","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 7","pages":"1261-1262"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13522","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-stabilized GIL1 suppresses PIN3 activity to inhibit hypocotyl gravitropism","authors":"Xiaolian Wang,&nbsp;Yanfang Yuan,&nbsp;Laurence Charrier,&nbsp;Zhaoguo Deng,&nbsp;Markus Geisler,&nbsp;Xing Wang Deng,&nbsp;Haodong Chen","doi":"10.1111/jipb.13736","DOIUrl":"10.1111/jipb.13736","url":null,"abstract":"<p>Light and gravity coordinately regulate the directional growth of plants. Arabidopsis Gravitropic in the Light 1 (GIL1) inhibits the negative gravitropism of hypocotyls in red and far-red light, but the underlying molecular mechanisms remain elusive. Our study found that GIL1 is a plasma membrane-localized protein. In endodermal cells of the upper part of hypocotyls, GIL1 controls the negative gravitropism of hypocotyls. GIL1 directly interacts with PIN3 and inhibits the auxin transport activity of PIN3. Mutation of <i>PIN3</i> suppresses the abnormal gravitropic response of <i>gil1</i> mutant. The GIL1 protein is unstable in darkness but it is stabilized by red and far-red light. Together, our data suggest that light-stabilized GIL1 inhibits the negative gravitropism of hypocotyls by suppressing the activity of the auxin transporter PIN3, thereby enhancing the emergence of young seedlings from the soil.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"1886-1897"},"PeriodicalIF":9.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13736","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9","authors":"Chenglu Zhao,&nbsp;Huanhuan Lou,&nbsp;Qian Liu,&nbsp;Siqi Pei,&nbsp;Qiansheng Liao,&nbsp;Zhenghe Li","doi":"10.1111/jipb.13741","DOIUrl":"10.1111/jipb.13741","url":null,"abstract":"<p>Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2079-2082"},"PeriodicalIF":9.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}