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IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 DOI: 10.1111/jipb.70382

引用次数: 0

A genotype-independent and highly efficient Agrobacterium-mediated soybean genetic transformation system 一个不依赖基因型的高效农杆菌介导的大豆遗传转化系统。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2025-12-03 DOI: 10.1111/jipb.70102

Fengxue Jing, Leping Geng, Yanyan Zhang, Hongtao Xie, Jian-Kang Zhu, Jianhua Zhu

引用次数: 0

Synergistic engineering of Casδ nuclease for robust genome editing Casδ核酸酶的协同工程用于稳健的基因组编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-03-15 DOI: 10.1111/jipb.70222

Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen

{"title":"Synergistic engineering of Casδ nuclease for robust genome editing","authors":"Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen","doi":"10.1111/jipb.70222","DOIUrl":"10.1111/jipb.70222","url":null,"abstract":"Casδ is a recently identified evolutionary transitional CRISPR system characterized by its compact size (~900 amino acids), broad temperature tolerance, and guidance by a short crRNA without the requirement of a tracrRNA. However, the low editing efficiency of Casδ in eukaryotic cells limits its application. Here, we have developed a hierarchical engineering strategy to improve the genome editing activity of Casδ-1, with optimization focused on enhancing its interactions with the crRNA, the protospacer adjacent motif (PAM) duplex, the single-stranded DNA substrate, and the RNA–DNA heteroduplex. Through this strategy, we successfully generated an activity-enhanced Casδ-1 variant, designated enCasδ, which harbors 9 amino acid substitutions that synergistically augment its editing efficiency. In human cell lines, enCasδ showed 1.3- to 29.3-fold higher editing activity than the wild-type Casδ-1 across ten tested genomic loci, with an average editing efficiency of 54.6%. In addition, enCasδ also mediated robust genome editing in maize; its editing efficiency increased by an average of 5.3-fold relative to Casδ-1, and reached up to an average of 80% at the TS4 and PSY1 loci in stable transgenic lines. The overall editing performance of enCasδ was comparable to that of Streptococcus pyogenes Cas9 (SpCas9) and other Cas12 nucleases. Collectively, enCasδ represents a highly optimized Casδ-1 variant that broadens the applicability of the Casδ CRISPR system and facilitates robust genome editing in both animal cells and plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3231-3242"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering the efficient Exo-Cas12i2 for MITE manipulation in rice 设计高效的Exo-Cas12i2用于水稻的螨虫处理。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-04-02 DOI: 10.1111/jipb.70254

Nan Chai, Wuge Liu, Kangli Sun, Qian Qian, Yao-Guang Liu, Xianrong Xie, Qinlong Zhu, Jiantao Tan

引用次数: 0

Optimization of a hypercompact Fanzor2 system for improved genome editing performance in plants 优化超紧凑Fanzor2系统，提高植物基因组编辑性能。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-07-14 DOI: 10.1111/jipb.70346

Xingyu Cao, Hongfei Liu, Shasha Bai, Rui Wang, Lanqin Xia, Yongwei Sun

引用次数: 0

Coupling of both a transactivation module and a double-stranded DNA-binding domain boosts Cas12i3 variant-based cytosine and adenine editing in plants transactivation模块和双链dna结合域的耦合促进了植物中基于Cas12i3变异的胞嘧啶和腺嘌呤编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-01-26 DOI: 10.1111/jipb.70154

Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia

{"title":"Coupling of both a transactivation module and a double-stranded DNA-binding domain boosts Cas12i3 variant-based cytosine and adenine editing in plants","authors":"Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia","doi":"10.1111/jipb.70154","DOIUrl":"10.1111/jipb.70154","url":null,"abstract":"CRISPR/Cas12i3 belongs to the type V-I Cas system, characterized by its smaller protein size and less restricted canonical “TTN” protospacer adjacent motif. Developments of Cas12i3-mediated base editing systems for either C-to-T or A-to-G transitions will expand the editing scope and enrich the plant base editing toolkits for crop improvement. However, while the Cas12i3-based cytosine base editor (CBE) only shows very low editing efficiency in plants, its adenine base editor (ABE) has not been documented as yet. Here, we engineered a series of Cas12i3 (5M)-based CBEs (V0–V5) and ABEs (V0–V5) by fusing a deactivated dCas12i3 (5M) with a transactivation module VP64, a single-stranded DNA-binding domain Rad51, or a double-stranded DNA-binding domain HMG-D, or in combinations, and systemically evaluated their performance in rice protoplasts. Our results demonstrated that synergistic combinations of both VP64 and HMG-D outperformed other architectures and significantly boosted the efficiencies of Cas12i3 (5M)-based CBE and ABE for C-to-T and A-to-G base editing and expanded the editing window. In stable lines, in comparison to the non-fusion control, the optimized Cas12i3 (5M)-based CBE-V5 and ABE-V5 enabled up to 4.78- and 3.35-fold higher editing efficiencies, with the maximum C-to-T and A-to-G efficiencies reaching 32.35% and 38.24%, respectively, and a higher proportion of homozygous mutants in the T0 generation. Furthermore, we generated herbicide-resistant rice germplasm by using CBE-V5 and ABE-V5, demonstrating their potential for precision breeding in crops. Together, here, we report novel Cas12i3 (5M)-based CBE and ABE that substantially enrich base editing toolkits for improvement of rice and potentially other crops.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3196-3207"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizations of Cas12a- and Cas12i-based adenine base editors for efficient precision editing in the plant genome 基于Cas12a-和cas12i的腺嘌呤碱基编辑器在植物基因组中高效精确编辑的优化

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-02-09 DOI: 10.1111/jipb.70177

Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei

引用次数: 0

CasY7: An optimized Cas12i system for enhanced genome editing in monocot crops CasY7：一种优化的Cas12i系统，用于单株作物的增强基因组编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-03-11 DOI: 10.1111/jipb.70181

Dating Zhong, Yanxiao Dong, Hong Pan, Yanan Fu, Yining Zhao, Shuting Ruan, Wencong Yu, Yiwen Wang, Qianlan Yin, Yanfei Zhang, Yisen Huang, Jiaqi Shen, Hongling Zhang, Yuxuan Wu, Jieting Xu, Yuming Lu

{"title":"CasY7: An optimized Cas12i system for enhanced genome editing in monocot crops","authors":"Dating Zhong, Yanxiao Dong, Hong Pan, Yanan Fu, Yining Zhao, Shuting Ruan, Wencong Yu, Yiwen Wang, Qianlan Yin, Yanfei Zhang, Yisen Huang, Jiaqi Shen, Hongling Zhang, Yuxuan Wu, Jieting Xu, Yuming Lu","doi":"10.1111/jipb.70181","DOIUrl":"10.1111/jipb.70181","url":null,"abstract":"<div>\u0000 \u0000 The CRISPR–Cas12 family nucleases, particularly the Cas12i subtypes, are considered promising alternatives to Cas9 for genome editing in plants. We previously developed a new Cas12i variant, CasY7, which has been successfully applied in clinical trials; its performance in plants remains to be investigated. Initial testing in stable transgenic maize and rice showed that the codon-optimized CasY7 (pCasY7e1) achieved average editing efficiencies of 58.7% and 62.3% across five target sites, respectively, outperforming the typical Cpf1 (pCpf1) control that targets the same sites. To further enhance activity, we fused T5 exonuclease to CasY7 (pCasY7e2), which shifted mutation profiles toward larger deletions, and subsequently integrated an MS2 aptamer into the crRNA scaffold (pCasY7e3). The optimized pCasY7e3 system increased editing efficiencies to 87.7% in maize and 82.9% in rice—approximately 2.7-fold higher than pCpf1. We further demonstrated multiplexed editing in maize, generating biallelic dwarf mutants, and validated functionality in hexaploid wheat with editing efficiencies up to 58.8%. Overall, our comprehensive validation across 942 transgenic plants confirmed robust editing in maize, rice, and wheat, establishing CasY7 as a high-efficiency addition to the CRISPR toolkit.</div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 9","pages":"3208-3219"},"PeriodicalIF":12.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of fragrant broomcorn millet (Panicum miliaceum L.) via CRISPR/Cas12i.3-mediated genome editing 利用CRISPR/Cas12i技术培育香黍（Panicum miliaceum L.）3介导的基因组编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-05-28 DOI: 10.1111/jipb.70295

Yuhe Bai, Baichuan Li, Jiawen Peng, Yan Bai, Shengnan Liu, Jinsheng Lai, Weibin Song

引用次数: 0

A novel Cas12b cytosine base editor enables efficient C-to-T base editing in the rice genome 一种新的Cas12b胞嘧啶碱基编辑器在水稻基因组中实现了高效的C-to-T碱基编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-05 Epub Date: 2026-07-19 DOI: 10.1111/jipb.70355

Yulei Wei, Lin He, Jienan Han, Shengnan Ge, Xueheyuan Wang, Xinyu Liu, Jie Deng, Haiyang Zhang, Xinyang Che, Jianfeng Weng, Wujun Ma, Jingyu Xu

引用次数: 0