Journal of Integrative Plant Biology最新文献

{"title":"The cytonuclear interactions during grapevine domestication","authors":"Ting Hou,&nbsp;Yanshuai Xu,&nbsp;Yang Dong,&nbsp;Jin Yao,&nbsp;Tianhao Zhang,&nbsp;Lianzhu Zhou,&nbsp;Xiangnian Su,&nbsp;Yi Zhang,&nbsp;Yingchun Zhang,&nbsp;Cheng Chen,&nbsp;Xiaoya Shi,&nbsp;Yuting Liu,&nbsp;Jiacui Li,&nbsp;Mengrui Du,&nbsp;Xinyue Fang,&nbsp;Sheng Yan,&nbsp;Sifan Yang,&nbsp;Wenrui Wang,&nbsp;Zhuyifu Chen,&nbsp;Siqi Qiao,&nbsp;Bilal Ahmad,&nbsp;Xiaodong Xu,&nbsp;Yanling Peng,&nbsp;Hua Xiao,&nbsp;Zhongxin Jin,&nbsp;Xiangpeng Leng,&nbsp;Cong Tan,&nbsp;Ling Tian,&nbsp;Chaochao Li,&nbsp;Yongfeng Zhou","doi":"10.1111/jipb.13968","DOIUrl":"10.1111/jipb.13968","url":null,"abstract":"<p>DNAs from the cytoplasmic genomes often communicate with the nuclear genome during regulation, development, and evolution. However, the dynamics of cytonuclear interaction during crop domestication have still been rarely investigated. Here, we examine cytonuclear interactions during grapevine domestication using pan-mitogenome, pan-plastome, and haplotype-resolved nuclear genomes, all assembled from long-read sequences across 33 wild and domesticated grapevine accessions. Structural variation shaped the mitogenomic variation in gene contents, leading to duplications of three specific genes during grapevine domestication (one <i>cox</i> and two <i>rpl</i> genes). Extensive genomic signals of cytonuclear interactions were detected, including a total of 212–431 nuclear–mitochondrial segments (NUMTs) and 95–205 nuclear–plastid segments (NUPTs). These results showed that NUMTs were under strong selection and were more abundant in cultivated grapes, whereas NUPTs dominated in wild grapes, indicating the evolutionary trajectories of cytonuclear interactions during grape domestication. Through Genome-Wide Association Study (GWAS), we identified 84 candidate genes associated with mitochondrial–nuclear genome interactions. Among these, the <i>PFD1</i> gene acts as a signaling regulator, modulating specific signaling pathways regulated by the mitochondria. Interestingly, there are significantly more cytonuclear interaction genes near NUMTs than in other genomic regions, suggesting NUMT-mediated interactions between the nuclear and mitochondrial genomes. Overall, our study provides evidence that NUMTs promote cytonuclear interaction during grapevine domestication, offering new insight into the impact of cytonuclear interactions on plant evolution, genetics, and breeding.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2686-2703"},"PeriodicalIF":9.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmTCP23 regulates leaf angle and tassel branch angle formation in maize by modulating LG1 expression and abscisic acid catabolism","authors":"Panpan Yang,&nbsp;Kailin Zeng,&nbsp;Hu Hailing Wang,&nbsp;Xiaoting Zhuang,&nbsp;Juntao Wu,&nbsp;Zerong Chen,&nbsp;Zhuojun Zhong,&nbsp;Yongming Liu,&nbsp;Dexin Kong,&nbsp;Haiyang Wang,&nbsp;Yuting Liu","doi":"10.1111/jipb.70000","DOIUrl":"10.1111/jipb.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>Leaf angle (LA) and tassel branch angle (TBA) are two important agronomic traits influencing maize plant architecture, thereby affecting its adaptability to high-density planting. <i>Liguleless1</i> (<i>LG1</i>) acts as a key regulator of LA and TBA, yet its precise regulatory mechanism remains largely obscure. In this study, we have identified ZmTCP23, a teosinte branched1/CYCLOIDEA/proliferating cell factors (TCP) transcription factor that is highly expressed in tassel and leaf primordia, serving as a pivotal upstream transcriptional regulator of <i>LG1</i>. The functional loss of <i>ZmTCP23</i> results in a significant reduction in both TBA and LA ranges. Moreover, <i>in vitro</i> and <i>in vivo</i> studies revealed that LG1 directly represses the expression of <i>ZmXERICO1</i>, a gene encoding an inhibitor of abscisic acid (ABA) degradation that can also influence LA and TBA upon overexpression. Additionally, ZmTCP23 physically interacts with the previously identified TBA regulator BAD1, forming a complex that co-activates the expression of <i>LG1</i> via direct binding to its promoter. This dynamic duo established a positive feedback loop, mutually enhancing each other's expression within the tassels, and consequently influencing TBA. Our findings establish a <i>ZmTCP23-LG1-ZmXERICO1</i> transcriptional regulatory cascade that orchestrates LA and TBA through influencing ABA content, and provide new targets for the genetic manipulation of LA and TBA for molecular breeding of high-density tolerant maize cultivars.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2744-2759"},"PeriodicalIF":9.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies reveal genetic diversity and regulatory loci underlying dwarfing traits in banana","authors":"Yuqi Li,&nbsp;Junting Feng,&nbsp;Liu Yan,&nbsp;Shouxing Wei,&nbsp;Huigang Hu,&nbsp;Juhua Liu,&nbsp;Yixian Xie,&nbsp;Bingyu Cai,&nbsp;Kai Li,&nbsp;Yankun Zhao,&nbsp;Yufeng Chen,&nbsp;Qifeng Cheng,&nbsp;Miaomiao Cao,&nbsp;Yi Wang,&nbsp;Yongzan Wei,&nbsp;Wei Li,&nbsp;Wei Wang,&nbsp;Jianghui Xie,&nbsp;Zhenhai Han","doi":"10.1111/jipb.70002","DOIUrl":"10.1111/jipb.70002","url":null,"abstract":"<p>Bananas (<i>Musa</i> ssp.) are globally important staple crops increasingly constrained by biotic stressors, climatic instability, and the high labor demands of cultivation. The genetic improvement of dwarf phenotypes offers a strategic pathway to enhance mechanization and reduce production costs. In this study, we have carried out whole-genome resequencing of 300 <i>Musa</i> accessions to analyze genome-wide allelic diversity and identify loci associated with shoot architecture. Our analysis uncovered extensive genetic variation within the A subgenome, pivotal for environmental adaptability, and detected introgression from <i>Musa itinerans</i> (subgroup A) into cultivated varieties (subgroup F), suggesting a broadened genetic base amenable to breeding. A genome-wide association study (GWAS) pinpointed <i>MabHLH30</i> as a crucial gene associated plant stature. Functional validation confirmed <i>MabHLH30</i> as a critical regulator of plant stature and leaf morphology. Leveraging this finding, we developed molecular markers for <i>MabHLH30</i>, enabling marker-assisted selection (MAS) to accelerate the breeding of compact, high-yielding cultivars. Collectively, these results provide a genomic framework for the targeted improvement of banana architecture and represent a valuable resource for cultivar development under diverse agroecological conditions.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2609-2623"},"PeriodicalIF":9.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOS2-FREE1 regulates SOS1 tonoplast sorting to promote Na+ compartmentalization in vacuole during salt stress response","authors":"Guoyong Liu,&nbsp;Xiang Yu,&nbsp;Yonglun Zeng,&nbsp;Baiying Li,&nbsp;Rong Wang,&nbsp;Xiangfeng Wang,&nbsp;Xiaoyun Zhao,&nbsp;Liwen Jiang,&nbsp;Yan Guo","doi":"10.1111/jipb.13970","DOIUrl":"10.1111/jipb.13970","url":null,"abstract":"<div>\u0000 \u0000 <p>Soil salinity significantly affects plant survival and limits crop productivity. Under salt stress, plants can transport sodium ions (Na⁺) out of cells and sequester them into vacuoles for detoxification. The salt excretion process is governed by the SALT OVERLY SENSITIVE (SOS) pathway, which involves the calcium sensors SOS3 and SOS3-LIKE CALCIUM BINDING PROTEIN 8, the protein kinase SOS2, and the plasma membrane Na⁺/H⁺ antiporter SOS1. While previous studies have provided insights into Na⁺ transport through the SOS system, the role of this pathway in Na⁺ compartmentalization within vacuoles remains poorly understood. In this study, we demonstrate that SOS1 partially internalizes to the tonoplast under salt stress, which is crucial for Na⁺ compartmentalization in vacuoles in Arabidopsis (<i>Arabidopsis thaliana</i>). We show that SOS2 phosphorylates the endosomal sorting complex required for transport-I (ESCRT-I) component FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which disrupts its interaction with VPS23A, an ESCRT-I component. This phosphorylation event inhibits the formation of intraluminal vesicles (ILVs) in prevacuolar compartments and multivesicular bodies (PVCs/MVBs), thereby remodeling endosomal sorting during salt stress. Additionally, our previous research indicated that SOS2-mediated phosphorylation of FREE1 leads to vacuole fragmentation by altering endomembrane fusion, thereby regulating intracellular Na⁺ homeostasis. Taken together, our findings reveal how the SOS2-FREE1 module orchestrates both endomembrane fusion and endosome sorting processes to enhance plant salt tolerance, providing novel insights into the cellular mechanisms underlying salt stress adaptation.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2545-2560"},"PeriodicalIF":9.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear phylogenomics provide evidence to clarify key morphological evolution and whole-genome duplication across rosids","authors":"Yiyong Zhao,&nbsp;Di Yu,&nbsp;Wenyu Kuo,&nbsp;Jie Huang,&nbsp;Jing Guo,&nbsp;Miao Sun,&nbsp;Yi Hu,&nbsp;Douglas E. Soltis,&nbsp;Pamela S. Soltis,&nbsp;Hong Ma,&nbsp;Chien-Hsun Huang","doi":"10.1111/jipb.13972","DOIUrl":"10.1111/jipb.13972","url":null,"abstract":"<div>\u0000 \u0000 <p>Rosids, comprising 90,000–120,000 species, form a large clade of angiosperms, including extensively studied families with many economically and scientifically important plants. They are also ecologically important, dominating many temperate and tropical ecosystems. Great progress in understanding rosid phylogenetic relationships has facilitated evolutionary studies, but phylogenetic uncertainties remain. To construct a more comprehensive nuclear phylogeny with expanded taxon coverage at the familial levels, we generated 203 new transcriptomes and two shotgun genomes. Along with other available data sets, our sample includes 419 eudicots, including 316 rosids, representing 83 families and all 16 rosid orders. Compared to the 1KP study, our highly resolved rosid phylogeny provides strongly supported internal relationships for one additional order and 16 families. We uncovered cytoplasmic-nuclear discordance for several deep rosid relationships with possible evidence of hybridization/gene flow and incomplete lineage sorting. By tracing ancestral states of morphological characters, we revealed putative floral evolutionary trends in some major clades. We detected strong evidence for 27 putative whole-genome duplication (WGD) events distributed across 20 rosid families, including five novel WGDs. Additionally, our expanded taxon sampling allowed for revised phylogenetic positions of several previously reported WGD events. Most of the supported WGDs correspond to origins of families or large subclades and occurred near times of geological and global climate upheavals, including those at the Cretaceous–Paleogene boundary. Our findings support the idea that large-scale genomic changes and key morphological innovations might have contributed to adaptive evolution and increased biodiversity in rosids.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2704-2730"},"PeriodicalIF":9.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term climate warming and nitrogen deposition increase leaf epiphytic and endophytic bacterial diversity","authors":"Lu Bai,&nbsp;Yunzhuo Wen,&nbsp;Guodong Han,&nbsp;Jinglei Tang,&nbsp;Zhuwen Xu,&nbsp;Zhongwu Wang,&nbsp;Lin Jiang,&nbsp;Haiyan Ren","doi":"10.1111/jipb.13965","DOIUrl":"10.1111/jipb.13965","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant microbiome plays a vital role in plant fitness and ecosystem functioning, yet its response to global environmental change remains poorly understood. Using an 18-year field experiment, we investigated the effects of climate warming and nitrogen deposition on the diversity of leaf epiphytic and endophytic bacterial communities in two dominant plant species (<i>Stipa breviflora</i> and <i>Cleistogenes songorica</i>) of a temperate desert steppe. We found that warming and nitrogen addition increased both epiphytic and endophytic bacterial diversity, but via different mechanisms. Specifically, epiphytic diversity increased with leaf temperature and transpiration rate, whereas greater endophyte diversity was linked to higher leaf carbon and nitrogen concentrations. Structural equation modeling revealed that both epiphytic and endophytic diversity were negatively associated with plant diversity. Our results demonstrate different mechanisms driving similar responses of leaf epiphytic and endophytic bacterial diversity to global change, and point to a negative feedback loop between phyllosphere bacterial and plant diversity.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 9","pages":"2430-2445"},"PeriodicalIF":9.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating morphological, anatomical, and physiological traits to explain elevational distributions in Himalayan steppe and alpine plants","authors":"Jan Binter,&nbsp;Martin Macek,&nbsp;Jiri Dolezal","doi":"10.1111/jipb.13971","DOIUrl":"10.1111/jipb.13971","url":null,"abstract":"<p>Understanding plant adaptive strategies that determine species distributions and ecological optima is crucial for predicting responses to global change drivers. While functional traits provide mechanistic insights into distribution patterns, the specific trait syndromes that best predict elevational optima, particularly in less-studied regions such as the Himalayas, remain unclear. This study employs a novel hierarchical framework integrating morphological, anatomical, and physiological traits to explain elevational distributions among 310 plant species across a 3,500-m gradient (2,650–6,150 m). We analyzed 95,000 floristic records collected from 4,062 localities spanning 80,000 km² in Ladakh, NW Himalayas, India, to define elevational optima and link them with 17 functional traits from over 7,800 individuals. We assessed the roles of moisture and cold limitations on trait–optima relationships by comparing two contrasting habitats (dry steppe and wetter, colder alpine). The predictive power of functional traits was more pronounced in the alpine species facing more extreme abiotic stress than the steppe species. Our results indicate that conservative life history strategies strongly predict elevational optima in alpine areas, while drought avoidance and competitive dominance are key in steppe habitats. Trait syndromes combining short stature, compact growth forms, enhanced storage tissues, and features promoting water-use efficiency (δ¹³C), freezing resistance (fructan levels), and nutrient retention (high root nitrogen and leaf phosphorus) explained 61% of the variation in alpine species' optima. Conversely, lifespan and clonal propagation determined the optima of steppe species at lower elevations. The study emphasizes the importance of functional trait combinations in determining elevational optima, highlighting that alpine species prioritize resource conservation and stress tolerance, while steppe species focus on competitive growth strategies. This multi-trait approach contrasts with previous research focusing on single trait–elevation relationships, providing novel insights into the diverse mechanisms shaping elevational distributions and offering valuable predictive power for assessing vegetation responses to future climate change.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2643-2657"},"PeriodicalIF":9.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORF proteins: A small family regulating organellar RNA editing and beyond","authors":"Jialong Li,&nbsp;Jiarui Yuan,&nbsp;Yanjun Jing,&nbsp;Rongcheng Lin","doi":"10.1111/jipb.13967","DOIUrl":"10.1111/jipb.13967","url":null,"abstract":"<div>\u0000 \u0000 <p>In the chloroplasts/plastids and mitochondria of flowering plants, RNA editing alters hundreds of cytidines to uridines at specific sites mediated by the editosome. Over the past decade, Multiple Organellar RNA Editing Factor (MORF) proteins have emerged as essential regulators that affect the editing efficiency of most editing sites in plastids and mitochondria. In Arabidopsis, the MORF family consists of nine members, each possessing a single conserved MORF-box that is distributed among flowering plants. Accumulating studies have demonstrated that MORF proteins interact with many other factors, including the PPR proteins and enzymes in different biosynthetic pathways, indicating that the MORF proteins play a more extensive role in regulating organellar development than RNA editing. Recent studies reveal that MORF2 and MORF9 possess holdase activity and may act as chaperones and that MORF8 undergoes heat-dependent phase separation to inhibit RNA editing in chloroplasts. In this review, we provide an overview of our current knowledge of the MORF family proteins and discuss the biological and molecular functions of this family in plants.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2532-2544"},"PeriodicalIF":9.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of cytosine and adenine base editors for maize precision breeding","authors":"Xiao Fu,&nbsp;Nan Wang,&nbsp;Lina Li,&nbsp;Dexin Qiao,&nbsp;Xiantao Qi,&nbsp;Changlin Liu,&nbsp;Zhaoxu Gao,&nbsp;Chuanxiao Xie,&nbsp;Jinjie Zhu","doi":"10.1111/jipb.13964","DOIUrl":"10.1111/jipb.13964","url":null,"abstract":"<p>Base editing technologies can improve crops, but their efficiency in maize remains suboptimal. This study attempts to overcome these limitations by examining optimized cytosine and adenine base editors (CBEs and ABEs), namely evoAPOBEC1, evoFERNY, evoCDA1, TadA8.20, and TadA8e, for precise genome editing in transient and stable expression maize cells. Employing a seed fluorescence reporter (SFR) system for rapid screening of BE transformants and transgene-free progenies, we enhanced editing efficiencies and heritability. Notably, TadA8.20 and evoCDA1 attained multiplexed editing efficiencies of up to 100.0% and 79.0% at the tested loci, respectively, with some homozygous and bi-allelic mutants exceeding 72.4% and 73.7%. Precise editing of <i>ZmACC1</i>/<i>2</i> (acetyl-CoA carboxylase) improved herbicide resistance, with <i>ZmACC2</i> mutants displaying improved performance. This study advances crop genetic engineering by facilitating robust, multi-locus modifications without altered agronomic performance, enhancing herbicide tolerance in maize. The successful utilization of these BE is a significant step forward in agricultural biotechnology and precision breeding.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2731-2743"},"PeriodicalIF":9.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying rubber-related genes through developing a sense/antisense RNA expression mutant library of Taraxacum kok-saghyz Rodin","authors":"Xiuli Fan,&nbsp;Qingwen Chen,&nbsp;Lianlian Hu,&nbsp;Chunyan Hai,&nbsp;Zepeng Hu,&nbsp;Junhui Zhang,&nbsp;Liquan Kou,&nbsp;Guodong Wang,&nbsp;Xiaoguang Song,&nbsp;Hong Yu,&nbsp;Xia Xu,&nbsp;Jiayang Li","doi":"10.1111/jipb.13969","DOIUrl":"10.1111/jipb.13969","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Taraxacum kok-saghyz</i> Rodin (TKS) is a promising alternative crop source for producing high-quality natural rubber (NR) and has become an ideal model plant for studying NR biosynthesis, regulation mechanisms, and production. So far, only a very limited number of functional genes related to NR biosynthesis have been identified in TKS. To achieve a systematic identification of its novel functional genes, we developed a mutant system denoted sense/antisense RNA expression (SARE) and have generated more than 8,000 transgenic TKS plants. A series of mutants with altered phenotypes, particularly changes in NR contents, were identified. To evaluate the efficiency of this library, we chose one mutant, <i>c112</i>, which exhibits a significant increase in NR content, for in-depth characterization. The <i>c112</i> mutant arose from the sense insertion of a <i>dormancy-associated gene1</i> (<i>DRM1</i>)/<i>auxin repressed protein</i> (<i>ARP</i>) gene, which we named <i>high natural rubber content1</i> (<i>HRC1</i>). In the <i>c112</i> mutant, the concentrations of NR precursors isopentenyl pyrophosphate and dimethylallyl diphosphate decreased, while geranylgeranyl diphosphate increased, suggesting that <i>HRC1</i> regulates metabolic flux in NR biosynthesis. In summary, the developed TKS SARE mutant library provides valuable genetic resources for identifying key functional genes to accelerate the domestication of TKS from wild species to economic crops through molecular breeding.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 10","pages":"2658-2667"},"PeriodicalIF":9.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}