Journal of Integrative Plant Biology最新文献_第6页

The CmMYB6-CmWRKY13-CmBr module mediates cucurbitacin B accumulation in melon fruit under CPPU treatment. CPPU处理下，CmMYB6-CmWRKY13-CmBr模块介导瓜素B在甜瓜果实中的积累。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-19 DOI: 10.1111/jipb.70388

Fei Luo, Haoxiong Tang, Yuqing Han, Liyuan Zhen, Ruirui Wei, Yushan Huang, Jian Pan, Tao Liu, Hongyan Qi

{"title":"The CmMYB6-CmWRKY13-CmBr module mediates cucurbitacin B accumulation in melon fruit under CPPU treatment.","authors":"Fei Luo, Haoxiong Tang, Yuqing Han, Liyuan Zhen, Ruirui Wei, Yushan Huang, Jian Pan, Tao Liu, Hongyan Qi","doi":"10.1111/jipb.70388","DOIUrl":"https://doi.org/10.1111/jipb.70388","url":null,"abstract":"Cucurbitacin B (CuB), a key compound responsible for the bitterness of melon fruit, significantly reduces fruit quality and consumer acceptance. Therefore, understanding the molecular mechanism underlying CuB biosynthesis is essential for improving melon fruit quality. In this research, we investigated CuB levels in the cmwrky13-knockout mutant following treatment with 20 mg L-1 CPPU (20CPPU) and hand pollination. The CuB content decreased by approximately 30% in the cmwrky13-knockout mutant compared with wild type under 20CPPU treatment. Yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays demonstrated that CmWRKY13 activates the promoter of CmBr, a key regulator of bitterness, and physically interacts with CmBr. Moreover, the nuclear-localized transcription factor CmMYB6 was specifically induced by 20CPPU treatment. Interestingly, CmMYB6 binds to the CmWRKY13 promoter and interacts with its protein, synergistically enhancing CuB gene expression. The cmmyb6-knockout mutants confirmed that CmMYB6 positively regulates CuB accumulation in melon fruit. Furthermore, CRISPR/Cas9-generated cmmyb6 cmwrky13 double mutants revealed that the CmMYB6-CmWRKY13 module controls CmBr expression and regulates CuB accumulation. Collectively, these findings elucidate the transcriptional cascade of CuB accumulation mediated by the CmMYB6-CmWRKY13-CmBr module in melon fruit, providing new insights into the molecular basis of bitterness formation and offering theoretical guidance for the breeding of non-bitter melon varieties.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149007590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T2T genome assembly and CRISPR/Cas9 editing of Medicago lupulina. 狼疮苜蓿T2T基因组组装及CRISPR/Cas9编辑

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-19 DOI: 10.1111/jipb.70401

Qing Yuan, Cheng Li, Guangyan Wang, Wenfei Dai, Eerdungaridi, Di Wu, Qingdun Fan, Yongping Yang, Yuanwen Duan, Xudong Sun

引用次数: 0

Four sequential filters constrain the efficacy of spray-induced gene silencing against fungal pathogens. 四个顺序过滤器限制了喷雾诱导的基因沉默对真菌病原体的功效。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-19 DOI: 10.1111/jipb.70400

Sooho Lim, Hokyoung Son

{"title":"Four sequential filters constrain the efficacy of spray-induced gene silencing against fungal pathogens.","authors":"Sooho Lim, Hokyoung Son","doi":"10.1111/jipb.70400","DOIUrl":"https://doi.org/10.1111/jipb.70400","url":null,"abstract":"Fungal pathogens remain a major constraint on crop production, while resistance and residue concerns increasingly limit reliance on conventional fungicides. Spray-induced gene silencing (SIGS), an RNA interference (RNAi)-based approach, offers a non-transgenic and sequence-specific strategy for controlling fungal diseases. Although laboratory studies increasingly report effective gene silencing and disease suppression, these results have not consistently translated into reliable protection under field-relevant conditions. In this review, we examine this gap through four sequential filters: environmental persistence, pathogen-site exposure, fungal cytosolic delivery, and RNAi machinery availability. We critically review the evidence relevant to each filter and assess how its importance varies among fungal pathosystems. Comparisons with commercially advanced RNAi products targeting non-fungal organisms illustrate how protected delivery and controlled exposure can mitigate several barriers to efficacy. This framework provides a practical basis for identifying pathosystem-specific bottlenecks and guiding the development of more reliable RNAi-based fungicides.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149007624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A robust biotechnology induces artificial genomic duplication via transient RNAi-mediated suppression of OSD1 in rice. 一种强大的生物技术通过rnai介导的水稻OSD1的瞬时抑制诱导人工基因组复制。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-16 DOI: 10.1111/jipb.70393

Zijun Lu, Lianjun Zhu, Guobin Liang, Rou Chen, Yu Huang, Jia Yang, Liang Fu, Xiangxing Zhao, Runze Wu, Xiangdong Liu

{"title":"A robust biotechnology induces artificial genomic duplication via transient RNAi-mediated suppression of OSD1 in rice.","authors":"Zijun Lu, Lianjun Zhu, Guobin Liang, Rou Chen, Yu Huang, Jia Yang, Liang Fu, Xiangxing Zhao, Runze Wu, Xiangdong Liu","doi":"10.1111/jipb.70393","DOIUrl":"https://doi.org/10.1111/jipb.70393","url":null,"abstract":"Ploidy manipulation is a crucial strategy for generating germplasm in crop breeding. However, artificial genomic duplication, often induced by colchicine treatment, is associated with toxicity and unpredictability. Although mutations in OSD1 have shown promise for inducing genomic duplication, the instability of ploidy across generations limits their practical application. In this study, we developed a Plant Polyploidization via Gene Interference (PPGI) system that utilizes transient RNAi-mediated suppression of OSD1 to efficiently induce artificial genomic duplication, demonstrating obvious potential for producing autotetraploids. We first validated this system by successfully generating PPGI-induced autotetraploid plants from the Taichung65 cultivar. These PPGI-induced plants exhibited notable differences from Taichung65 but resembled the existing Taichung65-4x line obtained through colchicine treatment. Haplotype analysis indicated that the OSD1 RNAi fragment is conserved across 2,908 rice cultivars. Consequently, we employed the same PPGI vector to develop autotetraploid lines from various germplasms, including another japonica cultivar, seven indica cultivars, and one Oryza rufipogon line. The probability of genomic duplication achieved by our PPGI method was higher than that obtained by colchicine treatment. Typically, autotetraploid lines exhibit severe sterility in the first generation following polyploidization. Leveraging fertile neo-tetraploid rice and the PPGI system, we designed and verified two strategies to directly induce fertile autotetraploid germplasms in the first generation, thereby substantially shortening the breeding cycle. Our method provides a universal, efficient, and non-toxic approach for inducing autotetraploid rice germplasms and contributes to enriching fertile autotetraploid rice germplasm resources.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced eugenol biosynthesis through GmEGS overexpression confers broad-spectrum pathogen protection in soybean. 通过过表达GmEGS增强丁香酚的生物合成，为大豆提供了广谱的病原体保护。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-16 DOI: 10.1111/jipb.70396

Menghua Zhang, Sen Yang, Ning Xu, Jingxue Li, Longxiaoran Liu, Yaxuan Zhang, Biao Ding, Tengfei Liu, Ming Wang, Bo Yang, Kaixuan Duan, Yuanchao Wang

{"title":"Enhanced eugenol biosynthesis through GmEGS overexpression confers broad-spectrum pathogen protection in soybean.","authors":"Menghua Zhang, Sen Yang, Ning Xu, Jingxue Li, Longxiaoran Liu, Yaxuan Zhang, Biao Ding, Tengfei Liu, Ming Wang, Bo Yang, Kaixuan Duan, Yuanchao Wang","doi":"10.1111/jipb.70396","DOIUrl":"https://doi.org/10.1111/jipb.70396","url":null,"abstract":"Soybean is a major source of plant protein and oil, yet its production is severely constrained by diverse pathogen infections. Here, we identify eugenol as a pathogen-induced defense metabolite in soybean with broad-spectrum antimicrobial activity against fungal, oomycete, and bacterial pathogens. Pathogen-associated molecular patterns (PAMPs), including Flg22, Chitin, and Elicitin, strongly induced soybean eugenol synthase (GmEGS) genes and promoted eugenol accumulation. Phylogenetic and expression analyses identified five GmEGS genes, among which GmEGS1a, GmEGS1c, and GmEGS2a were strongly induced by PAMPs and pathogen infection. Eugenol inhibited mycelial growth, spore germination, and zoospore release, while transcriptome analyses of Phytophthora sojae and Fusarium graminearum showed disruption of central carbon, amino acid, and membrane-associated metabolic pathways. In soybean, exogenous eugenol induced antioxidant metabolism and protein homeostasis without activating canonical immune responses, consistent with a protective effect associated with its antimicrobial activity rather than canonical immune activation. Overexpression of GmEGS1a or GmEGS1c increased endogenous eugenol accumulation and conferred broad-spectrum disease resistance without detectable growth penalties. Moreover, exogenous eugenol reduced disease severity in soybean, rice, maize, and tomato. Together, our findings establish GmEGS-mediated eugenol biosynthesis as an inducible chemical defense pathway and highlight eugenol as a promising natural antimicrobial compound for sustainable crop protection.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural variation in GmSOP5 regulates seed oil and protein content during soybean domestication. 在大豆驯化过程中，GmSOP5的自然变异调控着籽油和蛋白质含量。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-16 DOI: 10.1111/jipb.70392

Yu Tian, Hao Zhang, Zihao Zheng, Delin Li, Jindong Li, Yingpu Che, Xing Li, Changjiang Li, Huawei Gao, Long Yan, Chaosen Zhao, Jiantao Guan, Jianan Han, Qi Wang, Shiyu Guo, Dong Bai, Jochen C Reif, Ying-Hui Li, Li-Juan Qiu

{"title":"Natural variation in GmSOP5 regulates seed oil and protein content during soybean domestication.","authors":"Yu Tian, Hao Zhang, Zihao Zheng, Delin Li, Jindong Li, Yingpu Che, Xing Li, Changjiang Li, Huawei Gao, Long Yan, Chaosen Zhao, Jiantao Guan, Jianan Han, Qi Wang, Shiyu Guo, Dong Bai, Jochen C Reif, Ying-Hui Li, Li-Juan Qiu","doi":"10.1111/jipb.70392","DOIUrl":"https://doi.org/10.1111/jipb.70392","url":null,"abstract":"Seed oil content, protein content, and yield are agronomically important, correlated traits that determine the economic value of soybean (Glycine max). However, improving seed quality and yield simultaneously is challenging because gains in one breeding target often compromise the other, and the genetic basis of this trade-off is poorly understood. Here, we performed a genome-wide association study of 429 diverse soybean accessions and identified Seed Oil and Protein 5 (SOP5), which encodes a kinesin protein, as a key locus associated with seed oil and protein content. Knockout and overexpression experiments demonstrated that GmSOP5 positively affects seed oil content and 100-seed weight and negatively influences seed protein content. GmSOP5 is located in a selective sweep region, and the domestication-related GmSOP5H1 allele is nearly fixed in cultivated soybean, contributing to increased seed size, weight, and oil content and reduced protein content. Field trials demonstrated that neither loss-of-function GmSOP5-edited mutants, which have increased seed protein content, nor GmSOP5-overexpression lines, which have increased seed oil content, differed significantly in yield from wild-type plants, because changes in plant architecture were offset by changes in seed weight. Our results shed light on soybean domestication and suggest how pleiotropy can be harnessed in breeding to enhance seed quality without compromising yield.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An OsSK12/13-OsERF60-OsHsfA2c module regulates heat stress tolerance in rice. OsSK12/13-OsERF60-OsHsfA2c模块调节水稻耐热性。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-16 DOI: 10.1111/jipb.70397

Juan Gao, Zi-Wei Yao, Zhi-Ping Deng, Jing-Jing Wang, Dong-Jie Zhu, Dai-Xi Yue, Hai-Ping Lu, Jian-Xiang Liu

{"title":"An OsSK12/13-OsERF60-OsHsfA2c module regulates heat stress tolerance in rice.","authors":"Juan Gao, Zi-Wei Yao, Zhi-Ping Deng, Jing-Jing Wang, Dong-Jie Zhu, Dai-Xi Yue, Hai-Ping Lu, Jian-Xiang Liu","doi":"10.1111/jipb.70397","DOIUrl":"https://doi.org/10.1111/jipb.70397","url":null,"abstract":"Heat stress severely impairs plant growth and limits crop productivity. GSK3-like family proteins regulate diverse cellular processes, including plant responses to abiotic and biotic stresses. Nevertheless, their specific functions in heat stress responses remain poorly characterized. Here, we demonstrate that the expression of OsSK13 and the protein abundance of OsSK12/13 increase under heat stress in rice. Mutation in both OsSK12/13 compromises thermotolerance at both vegetative and reproductive stages, whereas overexpression of OsSK13 enhances seedling thermotolerance. We further identify that OsSK12/13 physically interact with and phosphorylate the APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factor OsERF60, and phosphorylation by OsSK12/13 enhances the stability of OsERF60. Consistent with its role in this regulatory module, OsERF60 loss-of-function mutants exhibit enhanced thermosensitivity at vegetative and reproductive stages. Expression profiling reveals that induction of heat stress-responsive genes, including OsHsfA2c, is reduced in both the OsSK12/13 double mutant and the OsERF60 mutant. OsERF60 directly binds to the OsHsfA2c promoter and activates its expression. Collectively, our findings uncover the OsSK12/13-OsERF60-OsHsfA2c regulatory module as a key component of the heat stress response in rice and provide mechanistic insights into thermotolerance in plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miR168a-TaAGO1b module improves Fusarium head blight resistance and yield in wheat. miR168a-TaAGO1b模块提高小麦赤霉病抗性和产量。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-14 DOI: 10.1111/jipb.70387

Guoliang Chen, Shuaifeng Geng, Zhongyin Deng, Yuqing Che, Ziying Wang, Tianhua Chen, Dada Cui, Xinyu Zou, Qihang Chen, Wanxin Xu, Zixu Zhao, Ziyi Yan, Tingting Li, Zili Ye, Jiajie Wu, Xigang Liu, Min Zhang, Long Mao, Aili Li, Shaoshuai Liu

{"title":"The miR168a-TaAGO1b module improves Fusarium head blight resistance and yield in wheat.","authors":"Guoliang Chen, Shuaifeng Geng, Zhongyin Deng, Yuqing Che, Ziying Wang, Tianhua Chen, Dada Cui, Xinyu Zou, Qihang Chen, Wanxin Xu, Zixu Zhao, Ziyi Yan, Tingting Li, Zili Ye, Jiajie Wu, Xigang Liu, Min Zhang, Long Mao, Aili Li, Shaoshuai Liu","doi":"10.1111/jipb.70387","DOIUrl":"https://doi.org/10.1111/jipb.70387","url":null,"abstract":"Fusarium head blight (FHB) is a devastating fungal disease of wheat that causes severe yield loss and mycotoxin contamination. Wheat varieties offering higher grain yield while maintaining moderate resistance to FHB are highly desirable. Here, we report that microRNA168a (miR168a) acts as a key negative regulator of both FHB resistance and grain yield in wheat. Knockdown of miR168a via target mimicry (MIM168) enhanced resistance to FHB in transgenic plants, accompanied by increased H2O2 accumulation and restricted fungal spread. MIM168 plants also exhibited higher grain yield with longer spikes and higher spikelet number per spike under uninfected field conditions. By contrast, miR168a overexpression (OE168) lines became more susceptible to FHB and displayed significantly reduced grain yield. We further identified TaAGO1b as a target of miR168a. Knockout of TaAGO1b using CRISPR/Cas9 (ago1b) recapitulated the FHB susceptibility and reduced spike length, demonstrating that miR168a and TaAGO1b work as a module. Moreover, miR168a positively regulates the miR156-TaSPL17 module, which is associated with spike development, and it also regulates miR444 in FHB infection, and that knockdown of miR444 via target mimicry (MIM444) enhances FHB resistance. Meanwhile, ago1b mutations upregulated miR156 and miR444. Thus, our data demonstrated that the miR168a-TaAGO1b module contributes to wheat spike architecture and FHB resistance via miR156 and miR444, providing a potential strategy for breeding high-yielding and FHB-resistant wheat varieties.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient genome editing in tomato using Cas12i3 variants. 利用Cas12i3变体对番茄进行高效基因组编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-14 DOI: 10.1111/jipb.70391

Siqun Wu, Lei Zhu, Meili Zhou, Bo Yu, Jinkai Li, Xiaomu Niu, Pengcheng Wang, Jian-Kang Zhu, Zhaobo Lang

引用次数: 0

Balancing high grain yield and early maturity: The OsFTL1 allele TGW1a^JZ. 平衡高产和早熟：OsFTL1等位基因TGW1aJZ。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-14 DOI: 10.1111/jipb.70389

Shaobo Wei, Yongqiang Liu, Wenbin Zhou

引用次数: 0