ZmTCP23通过调节LG1的表达和脱落酸分解代谢,调控玉米叶片角和穗枝角的形成。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Panpan Yang, Kailin Zeng, Hu Hailing Wang, Xiaoting Zhuang, Juntao Wu, Zerong Chen, Zhuojun Zhong, Yongming Liu, Dexin Kong, Haiyang Wang, Yuting Liu
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引用次数: 0

摘要

叶片角(LA)和穗枝角(TBA)是影响玉米植株构型的两个重要农艺性状,从而影响玉米对高密度种植的适应性。ligulless1 (LG1)是LA和TBA的关键调控因子,但其具体调控机制尚不清楚。在这项研究中,我们发现了一个在雄穗和叶片原基中高度表达的teosinte branched1/CYCLOIDEA/ proliferycell factors (TCP)转录因子ZmTCP23,它是LG1上游转录的关键调控因子。ZmTCP23的功能缺失导致TBA和LA范围的显著降低。此外,体外和体内研究表明,LG1直接抑制ZmXERICO1的表达,ZmXERICO1是一种编码脱落酸(ABA)降解抑制剂的基因,也可以影响LA和TBA的过表达。此外,ZmTCP23与先前鉴定的TBA调节因子BAD1物理相互作用,形成一个复合物,通过直接结合其启动子来共同激活LG1的表达。这对动态的二人组建立了一个正反馈循环,在流苏内相互增强彼此的表达,从而影响TBA。本研究建立了通过影响ABA含量调控LA和TBA的ZmTCP23-LG1-ZmXERICO1转录调控级联,为高密度耐药玉米品种LA和TBA的遗传操作提供了新的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ZmTCP23 regulates leaf angle and tassel branch angle formation in maize by modulating LG1 expression and abscisic acid catabolism.

Leaf angle (LA) and tassel branch angle (TBA) are two important agronomic traits influencing maize plant architecture, thereby affecting its adaptability to high-density planting. Liguleless1 (LG1) acts as a key regulator of LA and TBA, yet its precise regulatory mechanism remains largely obscure. In this study, we have identified ZmTCP23, a teosinte branched1/CYCLOIDEA/proliferating cell factors (TCP) transcription factor that is highly expressed in tassel and leaf primordia, serving as a pivotal upstream transcriptional regulator of LG1. The functional loss of ZmTCP23 results in a significant reduction in both TBA and LA ranges. Moreover, in vitro and in vivo studies revealed that LG1 directly represses the expression of ZmXERICO1, a gene encoding an inhibitor of abscisic acid (ABA) degradation that can also influence LA and TBA upon overexpression. Additionally, ZmTCP23 physically interacts with the previously identified TBA regulator BAD1, forming a complex that co-activates the expression of LG1 via direct binding to its promoter. This dynamic duo established a positive feedback loop, mutually enhancing each other's expression within the tassels, and consequently influencing TBA. Our findings establish a ZmTCP23-LG1-ZmXERICO1 transcriptional regulatory cascade that orchestrates LA and TBA through influencing ABA content, and provide new targets for the genetic manipulation of LA and TBA for molecular breeding of high-density tolerant maize cultivars.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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