SOS2-FREE1 regulates SOS1 tonoplast sorting to promote Na+ compartmentalization in vacuole during salt stress response.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Guoyong Liu, Xiang Yu, Yonglun Zeng, Baiying Li, Rong Wang, Xiangfeng Wang, Xiaoyun Zhao, Liwen Jiang, Yan Guo
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引用次数: 0

Abstract

Soil salinity significantly affects plant survival and limits crop productivity. Under salt stress, plants can transport sodium ions (Na+) out of cells and sequester them into vacuoles for detoxification. The salt excretion process is governed by the SALT OVERLY SENSITIVE (SOS) pathway, which involves the calcium sensors SOS3 and SOS3-LIKE CALCIUM BINDING PROTEIN 8, the protein kinase SOS2, and the plasma membrane Na+/H+ antiporter SOS1. While previous studies have provided insights into Na+ transport through the SOS system, the role of this pathway in Na+ compartmentalization within vacuoles remains poorly understood. In this study, we demonstrate that SOS1 partially internalizes to the tonoplast under salt stress, which is crucial for Na+ compartmentalization in vacuoles in Arabidopsis (Arabidopsis thaliana). We show that SOS2 phosphorylates the endosomal sorting complex required for transport-I (ESCRT-I) component FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which disrupts its interaction with VPS23A, an ESCRT-I component. This phosphorylation event inhibits the formation of intraluminal vesicles (ILVs) in prevacuolar compartments and multivesicular bodies (PVCs/MVBs), thereby remodeling endosomal sorting during salt stress. Additionally, our previous research indicated that SOS2-mediated phosphorylation of FREE1 leads to vacuole fragmentation by altering endomembrane fusion, thereby regulating intracellular Na+ homeostasis. Taken together, our findings reveal how the SOS2-FREE1 module orchestrates both endomembrane fusion and endosome sorting processes to enhance plant salt tolerance, providing novel insights into the cellular mechanisms underlying salt stress adaptation.

在盐胁迫响应中,SOS2-FREE1调控SOS1细胞质分选,促进液泡内Na+的区室化。
土壤盐分严重影响植物的生存,限制作物的生产力。在盐胁迫下,植物可以将钠离子(Na+)运输出细胞并将其隔离到液泡中进行解毒。盐排泄过程受盐过度敏感(SOS)通路控制,该通路涉及钙传感器SOS3和SOS3样钙结合蛋白8、蛋白激酶SOS2和质膜Na+/H+反转运蛋白SOS1。虽然以前的研究已经提供了通过SOS系统的Na+运输的见解,但这一途径在液泡内Na+区隔化中的作用仍然知之甚少。在这项研究中,我们证明了SOS1在盐胁迫下部分内化到拟南芥(Arabidopsis thaliana)的叶绿体中,这对Na+在液泡中的区隔至关重要。我们发现SOS2磷酸化了运输- i (esrt - i)组分所需的内体分选复合体(esrt - i),从而破坏了其与esrt - i组分VPS23A的相互作用。这种磷酸化事件抑制泡前室和多泡体(PVCs/MVBs)的腔内囊泡(ILVs)的形成,从而重塑盐胁迫下的内体分选。此外,我们之前的研究表明,sos2介导的FREE1磷酸化通过改变膜融合导致液泡破碎,从而调节细胞内Na+稳态。综上所述,我们的研究结果揭示了SOS2-FREE1模块如何协调膜融合和核内体分选过程,以增强植物的耐盐性,为盐胁迫适应的细胞机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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