Journal of Integrative Plant Biology最新文献

{"title":"Calcium signaling mediated by glutamate receptor-like protein PagGLR3.3 is involved in tension wood induction in poplar.","authors":"Yi An, Mei-Qiao Qu, Ya Geng, Xue Jiao, Xue-Qin Song, Shu-Tang Zhao, Xiao Han, Li-Chao Huang, Jin Zhang, Jie-Hua Wang, Suzanne Gerttula, Andrew Groover, Meng-Zhu Lu","doi":"10.1111/jipb.70158","DOIUrl":"10.1111/jipb.70158","url":null,"abstract":"<p><p>Tension wood (TW), a type of reaction wood that develops in angiosperm trees in response to gravistimulation, serves as an ideal model for investigating the regulatory mechanisms underlying xylem cell differentiation and cell wall deposition. The initial biological signals that induce the formation of reaction wood in response to gravitational stimuli remain poorly understood. In this study, we utilized pharmacological and genetic approaches to modulate Ca²⁺ levels in hybrid white poplar (Populus alba × P. glandulosa) and examine the role of calcium signaling during the early stages of gravitropic responses. Our findings revealed differential cytosolic Ca²⁺ signal distribution in gravistimulated stems during the early phase of gravity induction, characterized by lower Ca²⁺ levels on the upper side (where TW forms) and higher Ca²⁺ levels on the lower side (where opposite wood forms). Consistent with this hypothesis, plants treated with LaCl₃ and those with genetically disrupted calcium channels (PagGLR3.3 knockout using the CRISPR/Cas9 system) showed reduced Ca²⁺ signals and developed characteristic TW features. These results suggest that decreased Ca²⁺ levels induce the formation of TW. Furthermore, PagGLR3.3 knockout plants with TW-like stems displayed diminished sensitivity to gravistimulation. Transcriptomic analysis revealed that the knockout of PagGLR3.3 resulted in the upregulation of genes associated with TW formation and reactive oxygen species (ROS) production. Notably, superoxide anion (O₂ ^·-) levels were significantly elevated in the cambium zone of stems subjected to gravistimulation, LaCl₃ treatment, or PagGLR3.3 knockout, indicating that reduced Ca²⁺ levels promote TW formation through increased O₂ ^·- accumulation. This study offers novel insights into the critical role of Ca²⁺ in gravitropism and TW induction in poplar.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1349-1366"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E3 ubiquitin ligase-mediated degradation of Rab GTPase suppresses an MAPKK and activates immunity in rice.","authors":"Su Jiang, Ziwen Gong, Chenggang Li, Hui Tao, Feng He, Xiao Xu, Min Wang, Jisong Wang, Yuancheng Sun, Qin Feng, Zeyun Hao, Xiaoman You, Ruyi Wang, Jun Wu, Guo-Liang Wang, Yinghui Xiao, Yuese Ning, Dan Wang","doi":"10.1111/jipb.70149","DOIUrl":"10.1111/jipb.70149","url":null,"abstract":"<p><p>Small G proteins, functioning as monomeric GTPases, are critical molecular switches that regulate diverse processes in plants. However, little is known about their protein homeostasis during immune responses. Here, we demonstrate that OsRab11C1, encoding a Rab-type GTPase, is transcriptionally upregulated upon Magnaporthe oryzae infection. Strikingly, loss of OsRab11C1 enhances rice blast resistance, concomitant with increased defense gene expression, MAPK activation, and ROS burst. Mechanistically, we identify the E3 ubiquitin ligase EL5 as an interactor that ubiquitinates and targets OsRab11C1 for degradation via the 26S proteasome. Consistently, EL5 acts upstream of OsRab11C1 and positively regulates rice immunity. Further analysis reveals that OsRab11C1 interacts with and stabilizes mitogen-activated protein kinase kinase OsMKK6, thereby facilitating its autophosphorylation activity. In return, OsMKK6 acts as a negative regulator of rice programmed cell death and immunity. Collectively, our findings unveil a dynamic EL5-OsRab11C1-OsMKK6 signaling module that orchestrates rice immunity against pathogen invasion.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1491-1505"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146007953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-glycosylation profiles of apoplast nanoparticles modulate plant immune responses to different pathogens in Arabidopsis.","authors":"Guanting Niu, Hao Lu, Tunyu Jian, Xiaoqin Ding, Bei Tong, Yanan Gai, Xiuhua Meng, Han Lv, Mimi Li, Jian Chen","doi":"10.1111/jipb.70142","DOIUrl":"10.1111/jipb.70142","url":null,"abstract":"<p><p>Plants are constantly challenged by diverse pathogens and respond through multilayered immune mechanisms described by the classical \"zig-zag\" model. Apoplast nanoparticles (ANs), extracellular vesicle (EV)-like entities, are dynamically remodeled during plant-pathogen interactions. Although EVs are known to carry immune-regulatory cargos, the specificity and coordination of ANs protein responses to distinct pathogens remain largely unresolved. Here, we show that the glycosylation profiles of ANs and their N-glycoproteins undergo distinct remodeling upon infection with Pseudomonas syringae and Botrytis cinerea, as revealed by lectin microarray and mass spectrometry analyses. We identify SPILR, an ANs protein that modulates bacterial morphology and motility by reducing polygalacturonase activity and inhibiting the bacterial flagellar P-ring protein, FlgI. Additionally, the ESM1 protein enhances Arabidopsis resistance to B. cinerea through modulation of lipase activity and lipid metabolism. Disruption of N-glycosylation sites on ANs proteins compromises their antimicrobial function and alters host resistance to both bacterial and fungal pathogens. Together, our findings uncover N-glycosylation as a critical determinant of ANs-mediated extracellular immunity, highlighting glycosylation as an integrative mechanism linking vesicle biology, pathogen specificity, and immune signaling. This work establishes a framework for glycoengineering-based strategies to enhance crop resistance and advance nano-agricultural applications.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1469-1490"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural variation within duplicated AsWRKY49-D2 drives the subgenomic functional divergence of homeologs in salt response of allohexaploid oats.","authors":"Cailian Du, Yange Yun, Wenjia Li, Xiaolu Wu, Xingyu Liu, Minghao Li, Yingying Li, Shuhui Wang, Wei Li, Qiang He, Zhizhong Gong, Huilong Du, Qingbin Sun","doi":"10.1111/jipb.70171","DOIUrl":"10.1111/jipb.70171","url":null,"abstract":"<p><p>Salt stress is a major abiotic constraint limiting global crop production. Oat (Avena sativa L.), an allohexaploid cereal renowned for robust stress tolerance, remains poorly understood in terms of the molecular mechanisms underlying its response to salt stress. Here, we perform transcriptome profiling across multiple developmental stages and tissues of oat under salt stress, and construct the co-expression regulatory network to identify salt tolerance-associated gene modules. Notably, 10 salt-responsive transcription factor (SRTF) families with dynamic expression patterns are identified as core regulators, showing extensive subgenomic functional divergence, characterized by subgenome-dominant expression, as well as subgenome-specific duplication or loss events. Further integration with a genome-wide association study (GWAS) of the germination rate under salt stress in 225 oat accessions identified a 3-bp InDel variation within the duplicated gene AsWRKY49-D2, which specifically modulates its expression by facilitating binding of the TF AsZAT18, with AsWRKY49-D2 further mediating oat salt tolerance through targeted regulation of AsSOS2 and AsSOS3. Intriguingly, the salt-tolerant allele of AsWRKY49 is scarcely distributed in Chinese oat accessions, highlighting its considerable potential for breeding application. These results shed light on the regulatory mechanisms underlying oat salt tolerance, providing valuable information for exploring salt tolerance genes and breeding new salt-tolerant oat varieties.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1367-1383"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Rice blast pathogen effector AvrPib compromises disease resistance by targeting Raf-like protein kinase OsMAPKKK72 to inhibit MAPK signaling\".","authors":"","doi":"10.1111/jipb.70200","DOIUrl":"10.1111/jipb.70200","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1590"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147281563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The third root exudation pathway: Glutamine that leaks from defects in the endodermis recruits and assembles bacteria.","authors":"Yuchen Fei, Renfang Shen, Ping Lan","doi":"10.1111/jipb.70162","DOIUrl":"https://doi.org/10.1111/jipb.70162","url":null,"abstract":"<p><p>This commentary describes a study displaying the dynamic process of bacterial recruitment from plants through the leakage of glutamine from a defect in the endodermis layer. Leakage of glutamine from the root and bacterial chemotaxis both contribute to the colonization and proliferation of bacteria.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 5","pages":"1253-1255"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect infestation-induced autophagic degradation of OsPR1a fine-tunes rice salicylic acid defenses to benefit vector-borne virus transmission.","authors":"Jingya Zhao, Hongxiang Zhang, Yupeng Tang, Chunyu Zhang, Yuting Chen, Dongsheng Jia, Hongyan Chen, Taiyun Wei","doi":"10.1111/jipb.70166","DOIUrl":"10.1111/jipb.70166","url":null,"abstract":"<p><p>While plant salicylic acid (SA) signaling via NPR1-PR1 is well-characterized in pathogen resistance, its role against piercing-sucking insects remains unclear in rice. Here, we demonstrate that leafhopper infestation in rice induces SA-mediated resistance, which defends against insect infestation via pathogenesis-related protein OsPR1a. However, prolonged infestation triggers autophagy-dependent degradation of OsPR1a through its interaction with OsATG8b, fine-tuning immunity to prevent excessive defense activation. Strikingly, this autophagy-mediated OsPR1a degradation represents a conserved regulatory mechanism in rice during brown planthopper infestation. A rice rhabdovirus in leafhopper vectors secretes glycoprotein on virion envelopes to rice phloem, where it binds OsATG6b and OsPR1a to enhance autophagic OsPR1a turnover, ultimately facilitating insect vector feeding and viral transmission by leafhopper vectors. Our work reveals an adaptive mechanism by which a vector-borne virus hijacks plant autophagy to evade SA immunity, highlighting OsPR1a as a critical convergence point in plant-insect-virus interactions.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1523-1541"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.","authors":"Zhiqi Liu, Zizhen Liang, Mengfei Liao, Yixin Huang, Rui Ma, Jiayang Gao, Weiqi Wang, Tao Ni, Philipp S Erdmann, Liwen Jiang","doi":"10.1111/jipb.70143","DOIUrl":"10.1111/jipb.70143","url":null,"abstract":"<p><p>Understanding cellular events in three dimensions (3D) is of great importance for the annotation and illustration of biological processes in a contextual way. Imaging techniques based on electron microscopy (EM), such as those derived from scanning electron microscopy (SEM) and transmission electron microscopy (TEM), provide various options to visualize biological samples at scales ranging from cells to macromolecules in situ. Recently, a series of cryogenic techniques has brought EM-based imaging to a new level, enabling specimens to retain their hydrated state throughout the sample preparation and imaging steps, thereby offering a near-native visualization of cellular events. The application of dual-beam focused ion beam (FIB)-SEM to biological samples has enabled high-resolution reconstructions in 3D and streamlined sample preparation workflows for downstream cryo-electron tomography (cryo-ET) imaging. However, applications of these technologies to plant materials are limited due to intrinsic characteristics of plant cells (e.g., non-adhesive growth, large size with a central vacuole, and the presence of cell walls). For the timely application of dual-beam FIB-SEM in three-dimensional subcellular imaging of plant materials, we have recently tested and developed three major workflows with proof-of-concept evidence using developing anthers and in vitro-cultured pollen tubes based on Aquilos 2 Cryo-FIB, including (1) room-temperature FIB-SEM volume imaging, (2) cryo-lamellae preparation from cell suspension culture or high-pressure-frozen organs for cryo-ET imaging, and (3) cryo-FIB-SEM volume imaging, which will facilitate structural studies of plant materials and provide technical guidance for the broader plant cell biology research community.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1290-1314"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing tomato regeneration and genetic transformation efficiencies via exogenous chemical treatment and the GRF5 gene modulation.","authors":"Junjie Rong, Lei Zhu, Qingfeng Niu, Jianru Zhao, Fengjuan Chu, Jian-Kang Zhu, Zhaobo Lang","doi":"10.1111/jipb.70151","DOIUrl":"10.1111/jipb.70151","url":null,"abstract":"<p><p>Supplementation of Driver and Kuniyuki Walnut Medium with phloroglucinol enhanced regeneration efficiency in tomato tissue culture. Heterologous expression of an Arabidopsis growth-regulating factor gene, GROWTH-REGULATING FACTOR5 (GRF5), in tomato improved regeneration and transformation efficiency, suggesting a synergistic effect between phloroglucinol treatment and GRF-mediated pathways.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1268-1270"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146007947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The receptor-like cytoplasmic kinase PBL32 mediates SCOOP12 signaling from the MIK2-BAK1 receptor complex.","authors":"Yuanyuan Zhou, Kexin Chen, Jiaojiao Bai, Xiaotong Liu, Dongping Lu","doi":"10.1111/jipb.70139","DOIUrl":"10.1111/jipb.70139","url":null,"abstract":"<p><p>Upon recognition of the microbial or endogenous pattern SERINE-RICH ENDOGENOUS PEPTIDE12 (SCOOP12), the kinase PBL32 is phosphorylated and transmits immune signals downstream of the MIK2 receptor complex. Heterologous expression of Arabidopsis PBL32 and MIK2 in other plants enhances their disease resistance, offering a promising strategy for improving crop disease resistance.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"1265-1267"},"PeriodicalIF":9.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}