Journal of Integrative Plant Biology最新文献

{"title":"Stacking potato NLR genes activates a calcium-dependent protein kinase and confers broad-spectrum disease resistance to late blight.","authors":"Xiaoqiang Zhao, Fan Zhang, Xiaoqing Chen, Chongyuan Zhang, Haoyi Zhang, Tian Wang, Jinzhe Zhang, Cheng He, Shuo Wang, Xinjie Zhang, Xi Meng, Vladimir Nekrasov, Liang Kong, Suomeng Dong","doi":"10.1111/jipb.13892","DOIUrl":"https://doi.org/10.1111/jipb.13892","url":null,"abstract":"<p><p>Late blight, caused by the oomycete plant pathogen Phytophthora infestans, is a destructive disease that leads to significant yield loss in potatoes and tomatoes. The introgression of disease resistance (R) genes, which encode nucleotide-binding domain leucine-rich repeat-containing receptors (NLRs), into cultivated potatoes, is highly effective in controlling late blight. Here, we generated transgenic 2R and 3R potato lines by stacking R genes Rpi-blb2/Rpi-vnt1.1 and Rpi-vnt1.1/RB/R8, respectively, in the susceptible cv. Desiree background. The resulting 2R and 3R transgenic potato plants showed resistance to highly virulent P. infestans field isolates. We hypothesized that stacking R genes either resulted in up-regulation of a broader range of immune-related genes, or, more importantly, increase in the fold change of gene expression. To test our hypotheses, we performed transcriptome analysis and identified a subset of core immune-related genes that are induced in response to P. infestans in transgenic lines carrying single R genes versus lines carrying stacks of multiple R genes. In our analysis, stacking R genes resulted not only in the induction of a broader range of defense-associated genes but also a global increase in gene expression fold change, caused by the pathogen. We further demonstrated that the calcium-dependent protein kinase 16 (StCDPK16) gene significantly contributed to resistance to a virulent P. infestans strain, in the R gene background, in a kinase activity-dependent manner. Thus, our data suggest that stacking the R genes enhances late blight resistance through modulating the expression of a broader range of defense-related genes and highlights CDPK16 as a novel player in potato R gene-mediated resistance.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsPRMT5 methylates OsPAL1 to promote rice resistance, hindered by a Xanthomonas oryzae effector.","authors":"Cong Sheng, Kaihuai Li, Bo Wang, Wenchan Chen, Baodian Guo, Lulu Qiao, Hongwei Zhao, Yancun Zhao, Fengquan Liu","doi":"10.1111/jipb.13885","DOIUrl":"https://doi.org/10.1111/jipb.13885","url":null,"abstract":"<p><p>Rice bacterial blight, caused by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), poses a significant threat to rice crops. Arginine methylation, a post-translational modification of proteins, plays a pivotal role in transcriptional regulation, RNA processing, and the biosynthesis of plant hormones. Previous research has established that protein arginine methyltransferases (PRMTs) significantly influence protein function through arginine methylation. Nonetheless, the specific role of PRMT5 in regulating salicylic acid (SA) biosynthesis and plant immunity has been relatively unexplored. In this study, we elucidate the role of a rice protein arginine methyltransferase, OsPRMT5, in enhancing rice resistance to Xoo infection by interacting with the SA biosynthesis enzyme phenylalanine ammonia lyase 1 in rice (OsPAL1). Our results indicate that OsPRMT5 methylates OsPAL1 at the arginine residue 75, which affects the interaction between OsPRMT5 and OsPAL1 and subsequently boosts phenylalanine ammonia lyase (PAL) enzyme activity, leading to heightened SA accumulation. Conversely, compared to OsPAL1 overexpression plants in wild-type TP309 background, OsPAL1 overexpression plants in osprmt5 knockout (KO) mutants background exhibited diminished PAL activity. Furthermore, osprmt5 ospal1 double mutants demonstrated reduced resistance to bacterial blight compared to the OsPAL1-KO group. Additionally, we discovered that the Xoo effector protein PXO_01039 undermines the interaction between OsPRMT5 and OsPAL1, thereby facilitating Xoo infection. PXO_01039 binds to OsPRMT5, preventing the formation of the OsPRMT5-OsPAL1 complex, which results in decreased PAL activity and lower SA accumulation. In conclusion, our findings unveil how OsPRMT5 modulates the methylation and enzymatic activity of OsPAL1, a crucial enzyme in SA biosynthesis, to bolster plant antibacterial defenses.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional genomics dissection of the nodulation autoregulation pathway (AON) in soybean (Glycine max)","authors":"Peter M. Gresshoff,&nbsp;Chao Su,&nbsp;Huanan Su,&nbsp;April Hastwell,&nbsp;Yanyan Cha,&nbsp;Mengbai Zhang,&nbsp;Estelle B. Grundy,&nbsp;Xitong Chu,&nbsp;Brett J. Ferguson,&nbsp;Xia Li","doi":"10.1111/jipb.13898","DOIUrl":"10.1111/jipb.13898","url":null,"abstract":"<div>\u0000 \u0000 <p>The combination of mutation-based genetics and functional genomics has allowed a detailed dissection of the nodulation-induction and autoregulation of nodulation (AON) pathways of soybean. Applicable to all legumes, nodulation is induced by <i>Rhizobium</i>/<i>Bradyrhizobium-</i>produced lipopolysaccharides (Nod factors), perceived by Nod factor receptors (NFR1/NFR5 dimers), leading to cortical and pericycle cell divisions. These induce the production of CLAVATA3-like (CLE) peptides, which travel in the xylem to the shoot, where they are perceived by a receptor complex including a leucine-rich repeat (LRR) receptor kinase, encoded by <i>GmNARK</i>, <i>LjHAR1</i>, <i>MtSUNN</i> and closely related receptors in other legumes like <i>Phaseolus vulgaris</i> (common bean), <i>Pisum sativum</i> (pea), and <i>Glycine soja</i>. The activated receptor complex negatively regulates by phosphorylation of the constitutive synthesis of miR2111 in the shoot. This is normally is translocated via the phloem to the entire plant body, initiating suppression of a root-expressed Kelch repeat-containing F-box protein “Too Much Love (TML),” which in turn suppresses the nodule initiation cascade. Nodulation is therefore permitted during a developmental window between the induction and progress of the nodulation/cell division/infection cascade during the first few days after inoculation and the functional “readiness” of the AON cascade, delayed by the root–shoot–root loop. Loss-of-function mutations in <i>GmNARK</i> and <i>LjTML</i> result in excessive nodulation (supernodulation/hypernodulation/supernummary nodulation) as well as localized tolerance to externally applied nitrate. Recent analyses have indicated an interaction of the AON with lateral root formation as well as with the autoregulation of mycorrhization (AOM). Further details of the parallel functions of key points in this regulatory loop remain to be elucidated.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 3","pages":"762-772"},"PeriodicalIF":9.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the genomic divergence of maize heterotic groups in China.","authors":"Yingjie Xue, Yikun Zhao, Yunlong Zhang, Rui Wang, Xiaohui Li, Zhihao Liu, Weiwei Wang, Shaoxi Zhu, Yaming Fan, Liwen Xu, Wei Zhao, Jiuran Zhao, Fengge Wang","doi":"10.1111/jipb.13884","DOIUrl":"https://doi.org/10.1111/jipb.13884","url":null,"abstract":"<p><p>Diverse heterotic groups have been developed in China over several decades, but their genomic divergences have not been systematically studied after improvement. In this study, we performed Maize6H-60K array of 5,822 maize accessions and whole-genome re-sequencing of 150 inbred lines collected in China. Using multiple population structure analysis methods, we established a genetic boundary used to categorize heterotic groups and germplasm resources. We identified three chloroplast-cytoplasmic types that evolved during adaptation to diverse climatic environments in maize through phylogenetic and haplotype analyses. Comparative analyses revealed obvious genetic differences between heterotic groups and germplasm resources at both the chloroplast and nuclear genome levels, especially in the unique heterotic groups HG1 and HG2, which exhibited distinct regionality and genetic uniqueness. The divergent differentiation of heterotic groups from germplasm resources was driven by differential selection in specific genomic regions. Genome-wide selective sweep analysis identified core selected regions and candidate selected genes associated with traits between heterotic groups, highlighting that stress response- and plant defense-related genes were selected for environmental adaptation across a broad latitudinal range in China. Meanwhile, a genome-wide association study analysis provided evidence that core selected genes served as an important candidate gene pool with a potential role in genetic improvement. Gene exchanges among heterotic groups, which avoided the predominant heterotic patterns as much as possible, occurred to achieve population improvement during modern maize breeding. This study provides insights into the population differentiation and genetic characteristics of heterotic groups, which will facilitate the utilization of germplasm resources, the creation of novel maize germplasm, and the optimization of heterotic patterns during future maize breeding in China.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LUX-SWI3C module regulates photoperiod sensitivity in Arabidopsis thaliana.","authors":"Jianhao Wang, Huan Liu, Hong Li, Fan Wang, Songguang Yang, Lin Yue, Shuangrong Liu, Baohui Liu, Mingkun Huang, Fanjiang Kong, Zhihui Sun","doi":"10.1111/jipb.13889","DOIUrl":"https://doi.org/10.1111/jipb.13889","url":null,"abstract":"<p><p>In plants, the photoperiod sensitivity directly influences flowering time, which in turn affects latitudinal adaptation and yield. However, research into the mechanisms underlying photoperiod sensitivity, particularly those mediated by epigenetic regulation, is still in its nascent stages. In this study, we analyzed the regulation of photoperiod sensitivity in Arabidopsis thaliana. We demonstrate that the evening complex LUX ARRYTHMO (LUX) and the chromatin remodeling factor SWITCH/SUCROSE NONFERMENTING 3C (SWI3C) regulate GI locus chromatin compaction and H3K4me3 modification levels at the GIGANTEA locus under different photoperiod conditions. This mechanism is one of the key factors that allow plants to distinguish between long-day and short-day photoperiods. Our study provides insight into how the LUX-SWI3C module regulates photoperiod sensitivity at the epigenetic level.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DSD1/ZmICEb regulates stomatal development and drought tolerance in maize.","authors":"Wenqi Zhou, Jun Yin, Yuqian Zhou, Yongsheng Li, Haijun He, Yanzhong Yang, Xiaojuan Wang, Xiaorong Lian, Xiaoyun Dong, Zengke Ma, Liang Chen, Suiwen Hou","doi":"10.1111/jipb.13890","DOIUrl":"https://doi.org/10.1111/jipb.13890","url":null,"abstract":"<p><p>Maize (Zea mays L.) growth and yield are severely limited by drought stress worldwide. Stomata play crucial roles in transpiration and gas exchange and are thus essential for improving plant water-use efficiency (WUE) to help plants deal with the threat of drought. In this study, we characterized the maize dsd1 (decreased stomatal density 1) mutant, which showed defects in stomatal development, including guard mother cell differentiation, subsidiary cell formation and guard cell maturation. DSD1 encodes the basic helix-loop-helix transcription factor INDUCER OF CBF EXPRESSION b (ZmICEb) and is a homolog of ICE1 in Arabidopsis (Arabidopsis thaliana). DSD1/ZmICEb is expressed in stomatal file cells throughout stomatal development and plays a conserved role in stomatal development across maize and Arabidopsis. Mutations in DSD1/ZmICEb dramatically improved drought tolerance and WUE in maize and reduced yield losses under drought conditions. Therefore, DSD1/ZmICEb represents a promising candidate target gene for the genetic improvement of drought tolerance in maize by manipulating stomatal density.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential roles of nodule cysteine-rich peptides in maintaining the viability of terminally differentiated bacteroids in legume-rhizobia symbiosis.","authors":"Jian Yang, Fengzhan Gao, Huairong Pan","doi":"10.1111/jipb.13891","DOIUrl":"https://doi.org/10.1111/jipb.13891","url":null,"abstract":"<p><p>Investigations into the nitrogen-fixing symbiosis between legumes and rhizobia can yield innovative strategies for sustainable agriculture. Legume species of the Inverted Repeat-Lacking Clade (IRLC) and the Dalbergioids, can utilize nodule cysteine-rich (NCR) peptides, a diverse family of peptides characterized by four or six highly conserved cysteine residues, to communicate with their microbial symbionts. These peptides, many of which exhibit antimicrobial properties, induce profound differentiation of bacteroids (semi-autonomous forms of bacteria) within nodule cells. This terminal differentiation endows the bacteroids with the ability to fix nitrogen, at the expense of their reproductive capacity. Notably, a significant number of NCR peptides is expressed in the nodule fixation zone, where the bacteroids have already reached terminal differentiation. Recent discoveries, through forward genetics approaches, have revealed that the functions of NCR peptides extend beyond antimicrobial effects and the promotion of differentiation. They also play a critical role in sustaining the viability of terminally differentiated bacteroids within nodule cells. These findings underscore the multifaceted functions of NCR peptides and highlight the importance of these peptides in mediating communications between host cells and the terminally differentiated bacteroids.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular breeding of tomato: Advances and challenges","authors":"Minmin Du,&nbsp;Chuanlong Sun,&nbsp;Lei Deng,&nbsp;Ming Zhou,&nbsp;Junming Li,&nbsp;Yongchen Du,&nbsp;Zhibiao Ye,&nbsp;Sanwen Huang,&nbsp;Tianlai Li,&nbsp;Jingquan Yu,&nbsp;Chang-Bao Li,&nbsp;Chuanyou Li","doi":"10.1111/jipb.13879","DOIUrl":"10.1111/jipb.13879","url":null,"abstract":"<p>The modern cultivated tomato (<i>Solanum lycopersicum</i>) was domesticated from <i>Solanum pimpinellifolium</i> native to the Andes Mountains of South America through a “two-step domestication” process. It was introduced to Europe in the 16th century and later widely cultivated worldwide. Since the late 19th century, breeders, guided by modern genetics, breeding science, and statistical theory, have improved tomatoes into an important fruit and vegetable crop that serves both fresh consumption and processing needs, satisfying diverse consumer demands. Over the past three decades, advancements in modern crop molecular breeding technologies, represented by molecular marker technology, genome sequencing, and genome editing, have significantly transformed tomato breeding paradigms. This article reviews the research progress in the field of tomato molecular breeding, encompassing genome sequencing of germplasm resources, the identification of functional genes for agronomic traits, and the development of key molecular breeding technologies. Based on these advancements, we also discuss the major challenges and perspectives in this field.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 3","pages":"669-721"},"PeriodicalIF":9.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13879","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of serotonin in plant stress responses: Quo vadis?","authors":"Like Sun, Jiaxi Yin, Long Wang, Jingjing Li, Can Hu, Bo Liu, Chenfan Zheng, Jiale Chen, Vasileios Fotopoulos, Qingyao Shu, Meng Jiang","doi":"10.1111/jipb.13882","DOIUrl":"https://doi.org/10.1111/jipb.13882","url":null,"abstract":"<p><p>Serotonin (5-hydroxytryptamine (5-HT)) is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ, tissue, and cellular levels. It is considered increasingly important in regulating animal behavior, but the function of serotonin in plants is far less known. According to recent research, serotonin is vital for plant growth, development, and stress responses, achieved through transcriptional and phytohormonal interplay. Specifically, this review addresses critical gaps in the understanding of serotonin's function in plants by examining its biosynthesis, metabolism, and its multifaceted role in mitigating both abiotic stresses (salinity, drought, heat, cold, and heavy metals) as well as biotic challenges (pathogens, pests, and herbivores). As a pivotal player, it engages in a variety of significant cellular and molecular interactions, including those with reactive oxygen and nitrogen species (RONS), and various phytohormones such as auxin, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and cytokinin (CK). Advances in serotonin-related research are anticipated to offer a valuable basis for uncovering the regulatory pathways by which serotonin impacts the resilience of crops against abiotic stress.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered nanotransporters for efficient RNAi delivery in plant protection applications.","authors":"Yue Xing, Hao Jiang, Lin Cai","doi":"10.1111/jipb.13887","DOIUrl":"https://doi.org/10.1111/jipb.13887","url":null,"abstract":"<p><p>RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes plant tissue damage, is affected by environmental factors, and faces difficulties in penetrating the barriers of cell walls and the limitations of plant species, ultimately leading to low delivery efficiency. With advances in nanotechnology, nanomaterials (NMs) have been identified as effective carriers for nucleic acid delivery because of their ability to operate independently of external mechanical forces, prevent degradation by bioenzymes, exhibit good biocompatibility, and offer high loading capacity. This review summarizes the application of NM-mediated RNAi against plant pathogens and pests, focusing on how different NMs break through the cell barriers of plants, pathogens, and pests according to their size, morphology, and charge characteristics. Furthermore, we discuss the advantages and improvement strategies of NMs as nucleic acid delivery carriers, alongside assessing their potential application for the management of plant pathogens and pests.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}