Journal of Integrative Plant Biology最新文献_第2页

Creation of dual-purpose sorghum by CRISPR/Cas9-mediated knockout of Dry gene. CRISPR/ cas9介导的Dry基因敲除制备双用途高粱。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-07-19 DOI: 10.1111/jipb.70353

Fengyong Ge, Wenzhe Zhang, Kangxu Zhao, Baye Wodajo, Ran Xia, Feifei Yu, Qi Xie

引用次数: 0

A genotype-independent and highly efficient Agrobacterium-mediated soybean genetic transformation system. 一个不依赖基因型的高效农杆菌介导的大豆遗传转化系统。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2025-12-03 DOI: 10.1111/jipb.70102

Fengxue Jing, Leping Geng, Yanyan Zhang, Hongtao Xie, Jian-Kang Zhu, Jianhua Zhu

引用次数: 0

Intron editing of RISBZ1 confers thermotolerance for grain filling in rice. RISBZ1内含子编辑可提高水稻籽粒灌浆的耐热性。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-22 DOI: 10.1111/jipb.70274

Linhan Li, Lin Zhou, Hong Jiang, Long Huang, Kangwei Ji, Yijie Li, Zhonghao Yao, Ziang Li, Yongmei Bao, Ji Huang, Mengcen Wang, Wei Wang, Hongsheng Zhang, Sunlu Chen

引用次数: 0

Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica). 磁性纳米粒子介导枇杷遗传转化与基因编辑系统。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-05-28 DOI: 10.1111/jipb.70307

Xiaodong Suo, Haiyan Wang, Tingting Meng, Luwei Wang, Shuai Wang, Xun Xu, Shi Wang, Mubasshir Hussain, Danlong Jing, Jiangbo Dang, Di Wu, Qigao Guo

{"title":"Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica).","authors":"Xiaodong Suo, Haiyan Wang, Tingting Meng, Luwei Wang, Shuai Wang, Xun Xu, Shi Wang, Mubasshir Hussain, Danlong Jing, Jiangbo Dang, Di Wu, Qigao Guo","doi":"10.1111/jipb.70307","DOIUrl":"10.1111/jipb.70307","url":null,"abstract":"Loquat (Eriobotrya japonica Lindl.) is a valuable subtropical fruit tree whose genetic improvement has been significantly constrained by the absence of an efficient genetic transformation system. Although Agrobacterium-mediated transformation is the most widely used method, it proves ineffective in loquat due to the species' recalcitrance to in vitro regeneration. Pollen-based transformation offers a promising alternative by bypassing the need for tissue culture. However, the pollen wall poses a major physical barrier to the uptake of exogenous DNA. In this study, we investigated magnetic nanoparticle (MNP)-mediated transformation as a novel strategy for loquat. We confirmed that loquat pollen contains tricolporate apertures with diameters ranging from 3.0 to 5.0 μm, which are structurally suitable for the entry of MNPs-DNA. Based on this finding, we developed and optimized a transformation protocol using polyethyleneimine-coated Fe3O4 nanoparticles to deliver genetic material into loquat pollen grains. Using this approach, we successfully generated stable transgenic loquat lines, including both overexpression and gene-edited mutants. To our knowledge, this is the first report of successful MNP-mediated pollen transformation in a woody plant species. This work establishes a robust and efficient genetic transformation platform for loquat, providing a valuable tool for functional genomics and molecular breeding, as well as a potentially applicable strategy for other recalcitrant woody plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3272-3282"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148051271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A strategy for decreasing prolamin content in rice endosperm through gene editing. 通过基因编辑降低水稻胚乳中蛋白含量的策略。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-08-11 DOI: 10.1111/jipb.70371

Ziyan Shen, Youguang Li, Yuyan Huang, Tao Wang, Shijie Jian, Zihao Guan, Yuntong Feng, Xiangyuan Dang, Xinlan Shan, Shengyuan Sun, Min Guo, Zhi Hu, Yihao Yang, Changjie Yan

引用次数: 0

Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants. 增强的外切酶- cas9系统在植物中促进多核苷酸缺失，具有更高的效率和更广泛的靶向范围。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-01-21 DOI: 10.1111/jipb.70155

Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang

{"title":"Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants.","authors":"Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang","doi":"10.1111/jipb.70155","DOIUrl":"10.1111/jipb.70155","url":null,"abstract":"CRISPR-Cas9 is a widely used platform for plant genome editing, but its outcomes are typically dominated by small insertions and deletions (indels). Such limited mutation profiles restrict its utility in functional studies of non-coding RNAs and regulatory elements, such as microRNAs (miRNAs), untranslated regions (UTRs), and promoter sequences, where larger sequence disruptions are often required. Here, we developed enhanced exonuclease-Cas9 platforms, termed multiple nucleotide deletion Cas9 (MND-Cas9) systems, for efficient generation of large deletions in rice. By screening four exonucleases (RecJ, T5, TREX2, and SbcB), we established MND-Cas9v1 systems based on TREX2 or SbcB that produced substantially larger deletions without reducing editing efficiency. Further optimization with an inserted DNA-binding domain (DBD) between Cas9 and exonuclease yielded MND-Cas9v2, which simultaneously enhanced efficiency and deletion size. To expand PAM compatibility, we introduced PAM-relaxed Cas9-NG and SpG variants, generating MND-Cas9-NG/SpGv2 systems with broader targeting scope and superior performance compared to their parental nucleases. Finally, we demonstrated the utility of these systems in two applications: MND-Cas9v2 efficiently knocked out the miRNA gene OsMIR530, producing larger seeds, and generated extended deletions in the 3'UTR of OsGhd2, which upregulated its expression and increased grain size. These results demonstrate that MND-Cas9 systems enable high-efficiency generation of extended deletions and facilitate functional analyses of non-coding RNAs and regulatory sequences. Overall, this work establishes a versatile and expandable exonuclease-Cas9 platform that substantially broadens the mutational spectrum and application potential of CRISPR-Cas9 for plant genome engineering.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3316-3328"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic engineering of Casδ nuclease for robust genome editing. Casδ核酸酶的协同工程用于稳健的基因组编辑。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-03-15 DOI: 10.1111/jipb.70222

Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen

{"title":"Synergistic engineering of Casδ nuclease for robust genome editing.","authors":"Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen","doi":"10.1111/jipb.70222","DOIUrl":"10.1111/jipb.70222","url":null,"abstract":"Casδ is a recently identified evolutionary transitional CRISPR system characterized by its compact size (~900 amino acids), broad temperature tolerance, and guidance by a short crRNA without the requirement of a tracrRNA. However, the low editing efficiency of Casδ in eukaryotic cells limits its application. Here, we have developed a hierarchical engineering strategy to improve the genome editing activity of Casδ-1, with optimization focused on enhancing its interactions with the crRNA, the protospacer adjacent motif (PAM) duplex, the single-stranded DNA substrate, and the RNA-DNA heteroduplex. Through this strategy, we successfully generated an activity-enhanced Casδ-1 variant, designated enCasδ, which harbors 9 amino acid substitutions that synergistically augment its editing efficiency. In human cell lines, enCasδ showed 1.3- to 29.3-fold higher editing activity than the wild-type Casδ-1 across ten tested genomic loci, with an average editing efficiency of 54.6%. In addition, enCasδ also mediated robust genome editing in maize; its editing efficiency increased by an average of 5.3-fold relative to Casδ-1, and reached up to an average of 80% at the TS4 and PSY1 loci in stable transgenic lines. The overall editing performance of enCasδ was comparable to that of Streptococcus pyogenes Cas9 (SpCas9) and other Cas12 nucleases. Collectively, enCasδ represents a highly optimized Casδ-1 variant that broadens the applicability of the Casδ CRISPR system and facilitates robust genome editing in both animal cells and plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3231-3242"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering the efficient Exo-Cas12i2 for MITE manipulation in rice. 设计高效的Exo-Cas12i2用于水稻的螨虫处理。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-02 DOI: 10.1111/jipb.70254

Nan Chai, Wuge Liu, Kangli Sun, Qian Qian, Yao-Guang Liu, Xianrong Xie, Qinlong Zhu, Jiantao Tan

引用次数: 0

Optimization of a hypercompact Fanzor2 system for improved genome editing performance in plants. 优化超紧凑Fanzor2系统，提高植物基因组编辑性能。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-07-14 DOI: 10.1111/jipb.70346

Xingyu Cao, Hongfei Liu, Shasha Bai, Rui Wang, Lanqin Xia, Yongwei Sun

引用次数: 0

SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana. 拟南芥SIB1-SEC23A通过内质网到叶绿体的再定位介导免疫。

IF 12.5 1区生物学

Journal of Integrative Plant Biology Pub Date : 2026-09-01 Epub Date: 2026-04-12 DOI: 10.1111/jipb.70251

Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia

{"title":"SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana.","authors":"Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia","doi":"10.1111/jipb.70251","DOIUrl":"10.1111/jipb.70251","url":null,"abstract":"Inter-organellar communication has emerged as a critical factor in maintaining cellular homeostasis under stress conditions. Chloroplasts function not only as central organelles for energy production but are also increasingly recognized as stress sensors and signal integrators. SIGMA FACTOR-BINDING PROTEIN 1 (SIB1), encoded by a nuclear gene, has been identified as a positive regulator of plant immunity, with localization in both chloroplasts and the nucleus. In this study, we identified Arabidopsis SEC23A, a COPII complex component known to mediate membrane trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus, as a novel interactor of SIB1. Our findings reveal that under normal conditions, both SIB1 and SEC23A localize to the ER, while SIB1 is also localized in the nucleus. Upon ER stress and/or treatment with the immunity inducer salicylate, both proteins are relocated from the ER to the chloroplasts. Notably, SEC23A, similar to SIB1, also functions as a positive regulator of disease resistance. In response to pathogen infection, SIB1 and SEC23A downregulate expression of chloroplast- and nucleus-encoded genes associated with photosynthesis while enhancing expression of defense-related genes. Together, our findings reveal a previously uncharacterized pathway of ER-chloroplast communication mediated by SIB1 and SEC23A during plant stress responses and immunity, providing novel insights into the intricate regulatory networks that govern inter-organellar communication under stress in plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3458-3472"},"PeriodicalIF":12.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0