Journal of Integrative Plant Biology最新文献

{"title":"Asymmetric selection decoupling of nitrogen use efficiency in maize.","authors":"Li'an Jin, Jiafan Liu, Shan Jiang, Liqin Li, Jiajing Li, Gang Guo, Qingyun Zhang, Zhennan Xu, Zhigang Zheng, Junjie Zou, Baobao Wang, Haiyang Wang, Miaoyun Xu","doi":"10.1111/jipb.70412","DOIUrl":"https://doi.org/10.1111/jipb.70412","url":null,"abstract":"<p><p>Integrating phenotypic, genomic, and functional analyses identifies ZmLLG3 as a regulator of coordinated nitrogen acquisition and redistribution, highlighting a shift from single-trait selection toward coordinated optimization of nitrogen use efficiency.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NAC-MYB-cysteine regulatory module enhances drought and salt tolerance in soybean.","authors":"Nannan Zhang, Jiale Liu, Wenhuan Lv, Yanting Li, Yuanwen Zheng, Qi Shi, Fangguo Chang, Hu Zhang, Shihao Jia, Jiale Cui, Qican Cheng, Muqadas Aleem, Aisha Almakas, Tuanjie Zhao, Jianying Feng, Jinming Zhao","doi":"10.1111/jipb.70410","DOIUrl":"https://doi.org/10.1111/jipb.70410","url":null,"abstract":"<p><p>Drought and salinity are two major factors limiting soybean productivity worldwide, and both stresses can negatively affect plant growth in similar manners by disrupting cellular redox homeostasis and metabolism. However, the regulatory networks coordinating transcriptional control and metabolic adaptation under both types of stress remain poorly understood. Here, we demonstrate that the R2R3-MYB transcription factors GmMYB60a and GmMYB60b serve as core negative regulators of drought and salt tolerance in soybean. Loss-of-function mutations in GmMYB60a/b clearly increased stress tolerance, which was associated with reduced reactive oxygen species accumulation and increased antioxidant capacity. The GmMYB60 proteins directly bind to and transcriptionally repress GmCYS20, a key gene involved in cysteine biosynthesis, thereby reducing cellular cysteine and glutathione pools. Notably, the GmCYS20 protein physically interacts with GmMYB60 in the nucleus and weakens its ability to bind DNA, resulting in the formation of a transcription-metabolic feedback loop that stabilizes redox homeostasis under stress. Furthermore, the stress-responsive NAC transcription factor GmNAC3 directly suppresses GmMYB60 expression, thereby linking environmental signals to metabolic regulation. Together, these findings reveal a hierarchical GmNAC3-GmMYB60-GmCYS20 regulatory module that coordinates cysteine homeostasis and oxidative stress responses by integrating transcriptional repression with metabolic feedback. This work provides mechanistic insights into soybean stress adaptation and identifies promising genetic targets for improving soybean resistance to drought and salinity.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the tomato MARS1/ROUGH gene encoding a LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 in adventitious root and fruit skin formation","authors":"Eduardo Larriba,&nbsp;Cécile Bres,&nbsp;Aurora Alaguero-Cordovilla,&nbsp;Riyazuddin Riyazuddin,&nbsp;Qingyi Wang,&nbsp;Johann Petit,&nbsp;Jean-Philippe Mauxion,&nbsp;Lara Caballero,&nbsp;David Latrasse,&nbsp;Bénédicte Bakan,&nbsp;Rim Brik-Chaouche,&nbsp;David Esteve-Bruna,&nbsp;Moussa Benhamed,&nbsp;Christophe Rothan,&nbsp;José Manuel Pérez-Pérez","doi":"10.1111/jipb.70287","DOIUrl":"10.1111/jipb.70287","url":null,"abstract":"<p>In contrast to animals, plants possess a remarkable regenerative capacity and are capable of forming new organs or even entire organisms from a limited number of cells present in adult tissues, in response to injury or environmental changes. In this study, we describe the isolation and characterization of the <i>more adventitious roots1-1</i> (<i>mars1-1</i>) mutant, which exhibits enhanced regenerative potential upon wounding in tomato hypocotyl explants. Additionally, the <i>mars1-1</i> fruits exhibited a rough surface because of ectopic subepidermal cell proliferation, resulting in the formation of callus-like structures on their cuticle. The <i>MARS1/ROUGH</i> gene encodes a conserved lysine-specific histone demethylase, SlLDL1, which regulates processes such as cell proliferation, stem cell pluripotency, and embryogenesis in metazoans. Two CRISPR/Cas9 null alleles, <i>mars1-2</i> and <i>mars1-3</i>, were generated, and their pleiotropic phenotypes were characterized. ChIP-seq analysis revealed elevated levels of methylated lysine 4 in histone 3 within specific regions of the <i>mars1/rough</i> genome. To determine the impact of altered epigenetic marks on gene expression regulation in <i>mars1/rough</i> mutants, we analyzed the transcriptome of tomato hypocotyls during adventitious root formation. Using specific bioinformatic workflows and the resolution of the directional RNA-seq data, we identified several dozen distinct genomic regions with <i>de novo</i> expression in the <i>mars1/rough</i> mutants. One such region includes a novel B-type cyclin gene that is upregulated in <i>mars1/rough</i> mutants and may account for adventitious root and fruit skin phenotypes. Our findings indicate that SlLDL1 plays a role in maintaining silencing in specific genomic regions that are essential for tissue-specific reprogramming.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3644-3669"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering of rice endosperm for crocin biosynthesis.","authors":"Lijuan Wang, Jianjie Gao, Wenhui Zhang, Yongdong Deng, Hao Zhang, Rihe Peng, Bo Wang, Jing Xu, Hongjuan Han, Zhenjun Li, Yu Wang, Cen Qian, Yongsheng Tian, Quanhong Yao","doi":"10.1111/jipb.70246","DOIUrl":"10.1111/jipb.70246","url":null,"abstract":"<p><p>Crocins, the main active ingredients in Crocus sativus, have anti-inflammatory, antioxidant, hypolipidemic, hypoglycemic, and antitumor properties. Due to the harsh growing conditions and the limited yield of C. sativus, sustainable alternative methods for crocin production need to be explored. In this study, a new rice germplasm resource, \"crocin rice\", was developed by expressing eight genes of the crocin synthesis pathway in rice endosperm. The content of crocins in rice seeds was up to 9.25 μg/g dry weight, and it was also rich in a variety of carotenoids, including α-carotene, lutein, and violaxanthin. The expression of exogenous genes did not affect normal growth or the major nutritional structure of rice. Feeding experiments on mice showed that crocin rice could effectively reduce lipopolysaccharide-induced liver injury. With the adjuvant therapeutic benefits, crocin rice could be used to improve human nutrition and health. In addition, rice callus also showed the ability to synthesize crocins, which could serve as a potential substitute for C. sativus to alleviate resource shortage.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3765-3775"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic stomatal immunity: A rapid gate-closing circuit unveiled in plants","authors":"Fengling Wu,&nbsp;Fanrong Peng,&nbsp;Jing Guo,&nbsp;Ming Wu,&nbsp;Jianguo Wu","doi":"10.1111/jipb.70245","DOIUrl":"10.1111/jipb.70245","url":null,"abstract":"<p>This commentary highlights a recent study discovering systemic stomatal immunity, a rapid, targeted immune program, filling a research gap by showing local challenge triggers distal stomatal closure within hours.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3529-3531"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of diverse glycosyltransferases in Dracocephalum moldavica and engineering the production of bioactive flavonoid glycosides.","authors":"Dan-Dan Xu, Ni-Hong Du, Jia-Hui Li, Jiang-Nan Li, Jie Fu, Ming-Xin Cui, Rong Ni, Jiao-Zhen Zhang, Ying Lu, Ping Xu, Hong-Xiang Lou, Ai-Xia Cheng","doi":"10.1111/jipb.70212","DOIUrl":"10.1111/jipb.70212","url":null,"abstract":"<p><p>Dracocephalum moldavica L., an annual herb valued for its medicinal and ornamental properties, produces flavonoid glycosides like apigenin 7-O-glucuronide, scutellarein-7-O-glucuronide, and vitexin, which offer cardiovascular benefits. However, the UDP-glycosyltransferases (UGTs) involved in their biosynthesis have not been fully characterized. In the present investigation, we identified five UGTs, which comprise two bifunctional flavonoid UDP-glucuronosyl/glucosyltransferase genes, DmUGT1 and DmUGT2; two flavonoid UDP-glucosyltransferase genes, DmUGT3 and DmUGT4; and one type I di-C-glycosyltransferase gene, DmCGT1. The UDP-glucuronosyl/glucosyltransferase DmUGT1 showed effective glycosylation activity and exhibited a wide substrate promiscuity, facilitating the synthesis of the principal flavonoid glycosides in D. moldavica, including bioactive compounds such as scutellarein-7-O-glucuronide. Homology modeling and site-directed mutagenesis of the bifunctional DmUGT1 indicated that the amino acids Ser127 and Tyr373 are critical determinants of sugar donor specificity. DmCGT1 could catalyze phloretin to form phloretin-3'-C-glycoside and phloretin-3',5'-di-C-glycoside. Additionally, we engineered Escherichia coli strains that utilized DmUGT1 and DmCGT1, complemented with plasmids designed to enhance the intracellular supply of UDP-glucuronic acid and UDP-glucose in E. coli. These engineered strains successfully enabled the in vivo production of scutellarein-7-O-glucuronide and phloretin-3',5'-di-C-glycoside, achieving yields of 195 and 196 mg/L, respectively. This study provides a systematic elucidation of the glycosylation mechanisms of flavonoids in D. moldavica and offers candidate genes and methodologies for the biosynthesis of bioactive glycoside compounds through synthetic biology approaches.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":"3746-3764"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chicken or egg? The evolutionary riddle of CMS and Rf in plants","authors":"Peilin Wang,&nbsp;Zhe Zhao,&nbsp;Qibin Wu,&nbsp;Yaoguang Liu,&nbsp;Youxiong Que","doi":"10.1111/jipb.70292","DOIUrl":"10.1111/jipb.70292","url":null,"abstract":"<p>Cytoplasmic male sterility (CMS) reduces pollen fertility and nuclear Rf genes restore fertility. This Commentary explores support for the “arms race” and “pre-adaptation” models for CMS–<i>Rf</i> co-evolution. CMS and Rf interact dynamically, influencing plant reproduction, stress tolerance, and hybrid seed production, highlighting co-evolutionary processes critical for crop improvement.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3540-3542"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial organization of NLR signaling complexes in plant immunity","authors":"Chen Kang,&nbsp;Zhenyou Lou","doi":"10.1111/jipb.70293","DOIUrl":"10.1111/jipb.70293","url":null,"abstract":"<p>This commentary examines recent work that explores the spatial organization of the plant internal sensors that detect infection and trigger immune responses, showing form small units that assemble into larger clusters at the cell surface. This spatial organization may strengthen defense signals and lead to controlled cell death.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3543-3545"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147939830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinating nitrogen-responsive root and shoot growth for enhanced nitrogen-use efficiency","authors":"Xiaoming Zheng,&nbsp;Qian Qian","doi":"10.1111/jipb.70247","DOIUrl":"10.1111/jipb.70247","url":null,"abstract":"<p>The transcription factor WRINKLED1a coordinates nitrogen-responsive root and shoot growth in rice, improving nitrogen-use efficiency and yield stability.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3532-3534"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70247","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147588998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MYC2–EBF1–JAZ2 module bridges jasmonate and ethylene signals in apple","authors":"Xiao-Wei Zhang,&nbsp;Rui-Rui Xu,&nbsp;Lei Zhao,&nbsp;Yuepeng Han,&nbsp;Jian-Ping An","doi":"10.1111/jipb.70288","DOIUrl":"10.1111/jipb.70288","url":null,"abstract":"<p>MdEBF1, a negative regulator of ethylene signaling, promotes ubiquitination-mediated degradation of the jasmonate (JA) signaling repressor MdJAZ2 to release the key transcription factor MdMYC2 and initiate JA-enhanced disease resistance in apple, while JA-activated MdMYC2 upregulates <i>MdEBF1</i> transcription, establishing a positive feedback loop that amplifies JA signaling.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"68 10","pages":"3549-3551"},"PeriodicalIF":12.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.70288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}