Natural variations in MdBPM2/MdRGLG3-MdNAC83 network controlling the quantitative segregation of apple fruit storability.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bei Wu, Fei Shen, Ziying Zhou, Wenhui Ren, Yi Wang, Ting Wu, Zhenhai Han, Xinzhong Zhang
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引用次数: 0

Abstract

Dissecting quantitative traits into Mendelian factors is a great challenge in genetics. Apple fruit storability is a complex trait controlled by multi-genes with unequal effects. We previously identified 62 quantitative trait loci (QTLs) associated with apple fruit storability and genomics-assisted prediction (GAP) models were trained using 56 QTL-based markers. Here, three candidate genes, MdNAC83, MdBPM2, and MdRGLG3, were screened from the regions of QTLs with large G' value and large genetic effects. Both a 216-bp deletion and an SNP934 T/C at the promoter of MdNAC83 were associated with higher MdNAC83 expression but an SNP388 G/A at the coding region significantly reduced the activity to activate the expression of the target genes MdACO1, MdMANA3, and MdXTH28. MdBPM2 and MdRGLG3 participated in the ubiquitination of MdNAC83. SNP657 T/A of MdBPM2 and SNP167 C/G of MdRGLG3 caused a reduction in the activity to ubiquitinate MdNAC83. By the addition of functional markers to the GenoBaits SNP array, the prediction accuracy of the updated GAP models increased to 0.7723/0.6231 and 0.5639/0.5345 for flesh firmness/crispness at harvest and flesh firmness/crispness retainability, respectively. The variation network involving eight simple Mendelian variations in six genes helps to gain insight into the molecular quantitative genetics, to improve breeding strategy, and to provide targets for future genome editing.

控制苹果果实贮藏性定量分离的MdBPM2/MdRGLG3-MdNAC83网络的自然变异
在遗传学中,将数量性状分解为孟德尔因子是一个巨大的挑战。苹果果实的贮藏性是一种复杂的性状,受多基因控制,且作用不均衡。我们先前鉴定了62个与苹果果实贮藏性相关的数量性状位点(qtl),并使用56个基于qtl的标记训练了基因组学辅助预测(GAP)模型。本研究从具有较大G′值和较大遗传效应的qtl区域中筛选出3个候选基因MdNAC83、MdBPM2和MdRGLG3。MdNAC83启动子的216 bp缺失和SNP934 T/C均与MdNAC83的高表达相关,但编码区SNP388 G/ a显著降低了靶基因MdACO1、MdMANA3和MdXTH28的激活活性。MdBPM2和MdRGLG3参与了MdNAC83的泛素化。MdBPM2的SNP657 T/A和MdRGLG3的SNP167 C/G导致MdNAC83泛素化活性降低。通过在GenoBaits SNP序列中添加功能标记,更新后的GAP模型对收获时肉韧度/脆度和肉韧度/脆度保存性的预测精度分别提高到0.7723/0.6231和0.5639/0.5345。该变异网络涉及6个基因中的8个简单孟德尔变异,有助于深入了解分子定量遗传学,改进育种策略,并为未来的基因组编辑提供目标。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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