Journal of Integrative Plant Biology最新文献_第3页

Tomato TGase positively regulates thermotolerance by inducing polyamine to activate autophagy. 番茄TGase通过诱导多胺激活自噬，正向调节番茄耐热性。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-07-03 DOI: 10.1111/jipb.13955

Min Zhong, Qingshen Cui, Yan Yang, Ke Zhang, Xiaoying Liu, Guan Pang, Lifei Yang, Shirong Guo, Jin Sun, Yu Wang

{"title":"Tomato TGase positively regulates thermotolerance by inducing polyamine to activate autophagy.","authors":"Min Zhong, Qingshen Cui, Yan Yang, Ke Zhang, Xiaoying Liu, Guan Pang, Lifei Yang, Shirong Guo, Jin Sun, Yu Wang","doi":"10.1111/jipb.13955","DOIUrl":"https://doi.org/10.1111/jipb.13955","url":null,"abstract":"Transglutaminases (TGases) are multifunctional enzymes involved in stress responses, while autophagy is a key cellular degradation process. However, the relationship between TGases and autophagy in the plant heat stress response remains poorly understood. In this study, we demonstrated that TGase was essential for heat tolerance by regulating autophagy. Heat stress induced both TGase expression and activity. The tgase mutants reduced, while TGase-overexpression (TGaseOE) lines increased plant thermotolerance. Under heat stress, insoluble proteins were more ubiquitinated in tgase mutants and less so in TGaseOE plants. Moreover, TGase promoted the expression of autophagy-related (ATG) genes and autophagosome formation. Polyamine content and the expression of polyamine-related genes, particularly SAMS2, were positively correlated with TGase activity. TGase interacted with SAMS2 both in vitro and in vivo, and knockout of SAMS2 impaired TGase-induced thermotolerance and autophagosome formation in TGaseOE plants. Exogenous spermidine also promoted autophagosome formation in tgase mutants, indicating a critical role of polyamine in TGase-mediated heat tolerance and autophagosome formation. Furthermore, a cell-free degradation assay showed that TGase enhanced the stability of SAMS2. Altogether, these results reveal that TGase interacts with and stabilizes SAMS2 to promote polyamine synthesis, which upregulates ATG gene expression and facilitates autophagosome formation to degrade ubiquitinated proteins, thereby enhancing the thermotolerance of tomato plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation for adaptive evolution in response to changed environments in plants. 植物适应环境变化的遗传变异。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-07-03 DOI: 10.1111/jipb.13961

Jing Hou, Meng Liu, Kai Yang, Bao Liu, Huanhuan Liu, Jianquan Liu

{"title":"Genetic variation for adaptive evolution in response to changed environments in plants.","authors":"Jing Hou, Meng Liu, Kai Yang, Bao Liu, Huanhuan Liu, Jianquan Liu","doi":"10.1111/jipb.13961","DOIUrl":"https://doi.org/10.1111/jipb.13961","url":null,"abstract":"Plants adapt to their local environments through natural or artificial selection of optimal phenotypes. Recent advances in genomics and computational biology, which integrate phenotypic and multi-omics data, have facilitated the rapid identification of key genes and allelic variations that underlie these adaptive evolutionary processes. Understanding the underlying molecular mechanisms has significantly enhanced our knowledge of how plants respond to changed habitats, including various biotic and abiotic stresses. In this review, we highlight recent progress in elucidating the genetic basis of phenotypic variation in morphological traits and stress responses, as well as the emergence of new ecotypes, subspecies, and species during adaptive evolution across varied environments. This occurs through allelic divergences in both coding and non-coding regions in both model and non-model plants. Furthermore, the terrestrialization and early diversification of land plants involved the acquisition of additional genes, primarily through horizontal gene transfer and whole-genome duplication, which facilitated the development of complex molecular pathways to adapt to increasingly diverse environments. Finally, we discuss emerging trends and prospects for exploring and utilizing beneficial alleles for environmental adaptation, to guide crop breeding efforts in response to global climate change.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A plant viral effector disrupts ALD1-OSB1 immunity module to suppress chloroplast defenses. 植物病毒效应物破坏ALD1-OSB1免疫模块抑制叶绿体防御。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-07-03 DOI: 10.1111/jipb.13959

Zuxian Pan, Yaqin Wang, Fangfang Li, Yuzhen Mei, Xueping Zhou

{"title":"A plant viral effector disrupts ALD1-OSB1 immunity module to suppress chloroplast defenses.","authors":"Zuxian Pan, Yaqin Wang, Fangfang Li, Yuzhen Mei, Xueping Zhou","doi":"10.1111/jipb.13959","DOIUrl":"https://doi.org/10.1111/jipb.13959","url":null,"abstract":"Chloroplasts are central to plant immunity, with the chloroplast-localized protein AGD2-LIKE DEFENSE RESPONSE PROTEIN 1 (ALD1) playing a critical role in producing pipecolic acid (Pip), a key immune signal. However, the regulation of ALD1 and how pathogens evade ALD1-mediated defenses remain poorly understood. Using the geminivirus tomato yellow leaf curl China virus and its associated betasatellite (TYLCCNV/TYLCCNB) as a model, we uncovered a defense mechanism involving organellar single-stranded DNA-binding protein 1 (OSB1), which stabilizes ALD1 and promotes Pip biosynthesis to strengthen immunity. Crucially, the viral βC1 effector encoded by TYLCCNB disrupts this pathway by binding OSB1 and sequestering it away from chloroplasts, thereby blocking OSB1-ALD1 interaction, destabilizing ALD1, and suppressing Pip-dependent defenses. Strikingly, βC1 mutants defective in OSB1 binding fail to interfere with the OSB1-ALD1 stability, and TYLCCNV infections carrying these mutants induce attenuated symptoms in Nicotiana benthamiana. Our study not only reveals how ALD1-OSB1 cooperates in chloroplast immunity but also demonstrates how geminiviruses, as a tractable model, can dissect pathogen counter-defense strategies.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial complex I assembles via a distal-pump module (P_D)-early and P_D-late pathway in maize. 玉米线粒体复合体I通过远端泵模块（PD）早期和PD晚期途径组装。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-07-03 DOI: 10.1111/jipb.13958

Baoyin Chen, Manna Huang, Junjun Wang, Yuanye Gui, Qingqing Wei, Zhihui Xiu, Feng Sun, Yan-Zhuo Yang, Chunhui Xu, Bao-Cai Tan

{"title":"Mitochondrial complex I assembles via a distal-pump module (PD)-early and PD-late pathway in maize.","authors":"Baoyin Chen, Manna Huang, Junjun Wang, Yuanye Gui, Qingqing Wei, Zhihui Xiu, Feng Sun, Yan-Zhuo Yang, Chunhui Xu, Bao-Cai Tan","doi":"10.1111/jipb.13958","DOIUrl":"https://doi.org/10.1111/jipb.13958","url":null,"abstract":"The L-shape mitochondrial complex I (CI) consists of four modules: nicotinamide-adenine dinucleotide hydrogen-binding module (N) and ubiquinone-binding module (Q) in the matrix arm and proximal-pump module (PP) and distal-pump module (PD) in the membrane arm. As mitochondrial mutants are unavailable, the CI assembly pathway in plants is unclear. We investigated the CI assembly process using the maize RNA processing mutants deficient in individual CI components. Complexome profiling detected all major assembly intermediates of each module, confirming their independent assembly pathway. A block in the Q module assembly causes the accumulation of the membrane arm, whereas a block in the PD module assembly results in the accumulation of CI*, a subcomplex assembled by PP with the matrix arm. We further isolated and analyzed the mutants of two CI assembly factors, ZmGLDH and CRK1. The absence of either ZmGLDH or CRK1 eliminates the accumulation of CI* but allows a substantial amount of CI to be assembled. The membrane arm was also accumulated in zmgldh and crk1. Together, these results suggest two CI assembly pathways. The PD-early former starts with the assembly of PP with PD, forming the membrane arm, then joins with the matrix arm to produce CI. The PD-late pathway is initiated by assembling PP with the matrix arm to yield CI*, which is then combined with PD to form CI.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TaIRE1-mediated unconventional splicing of the TabZIP60 mRNA and the miR172 precursor regulates heat stress tolerance in wheat. taire1介导的TabZIP60 mRNA和miR172前体的非常规剪接调节小麦的耐热性。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-07-03 DOI: 10.1111/jipb.13963

Haoran Li, Zhen Qin, Xiaoli Geng, Jie Cao, Xinyang Yuan, Huiru Peng, Yingyin Yao, Zhaorong Hu, Weilong Guo, Yumei Zhang, Jie Liu, Vincenzo Rossi, Ive De Smet, Zhongfu Ni, Qixin Sun, Mingming Xin

{"title":"TaIRE1-mediated unconventional splicing of the TabZIP60 mRNA and the miR172 precursor regulates heat stress tolerance in wheat.","authors":"Haoran Li, Zhen Qin, Xiaoli Geng, Jie Cao, Xinyang Yuan, Huiru Peng, Yingyin Yao, Zhaorong Hu, Weilong Guo, Yumei Zhang, Jie Liu, Vincenzo Rossi, Ive De Smet, Zhongfu Ni, Qixin Sun, Mingming Xin","doi":"10.1111/jipb.13963","DOIUrl":"https://doi.org/10.1111/jipb.13963","url":null,"abstract":"INOSITOL-REQUIRING ENZYME 1 (IRE1) is conserved in plants and mammals to regulate stress responses. Here, we found that TaIRE1 is involved in the unconventional splicing of cell membrane-localized TabZIP60 messenger RNA (mRNA), which results in a nucleus resident protein form (TabZIP60s), and enhanced heat stress tolerance. Transcriptome analysis together with binding element prediction revealed 121 high-confidence targets of TabZIP60s responsive to heat stress in wheat (Triticum aestivum), including heat shock protein genes. Interestingly, we found that an asparagine to glutamic acid substitution, located next to DNA-binding domain of TabZIP60s, results in reduced binding affinity and transcriptional activity to downstream targets, and this heat stress tolerance inferior allele was positively selected during modern wheat breeding programs in China, possibly due to their negative effects on yield potential. Finally, we showed that TaIRE1 is also responsible for the mis-cleavage of miR172 precursors, and consequently contribute to heat stress tolerance. To the best of our knowledge, this represents the first report showing that, like in mammals, IRE1 also regulates miRNA cleavage in response to heat stress in plants. Together, this coordinate control of two signaling pathways provides new insights into heat stress tolerance regulation in wheat.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf multi-dimensional stoichiometry as a robust predictor of productivity on the Tibetan Plateau. 叶片多维化学计量作为青藏高原生产力的可靠预测因子。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-06-27 DOI: 10.1111/jipb.13960

Xin Li, Jiahui Zhang, Kathrin Rousk, Yinghua Zhang, Yi Jiao, Pu Yan, Nianpeng He

{"title":"Leaf multi-dimensional stoichiometry as a robust predictor of productivity on the Tibetan Plateau.","authors":"Xin Li, Jiahui Zhang, Kathrin Rousk, Yinghua Zhang, Yi Jiao, Pu Yan, Nianpeng He","doi":"10.1111/jipb.13960","DOIUrl":"https://doi.org/10.1111/jipb.13960","url":null,"abstract":"Accurately predicting gross primary productivity (GPP) is crucial for understanding carbon cycling; however, most studies have predominantly investigated GPP using only environmental metrics, overlooking the pivotal role of functional traits as intermediaries between the environment and GPP and the predictive potential of GPP. Therefore, this study developed a three-dimensional \"engine\" framework to predict GPP and tested it by leveraging functional traits from 2,040 plant communities on the Tibetan Plateau, incorporating environmental factors and the length of the plant-growing season. Our results highlight that while the environment exerts a dominant direct influence on GPP dynamics, the contribution of leaf density traits to GPP prediction should not be overlooked. The proposed framework achieved a prediction accuracy close to 0.92, underscoring its feasibility in GPP prediction. However, incorporating the nitrogen-to-phosphorus ratio into the framework diminished the model's predictive accuracy. Within the stoichiometric dimension alone, the prediction accuracy significantly increased with the number of input traits, indicating a substantial potential for enhancing predictive capability. In the dimension of environmental factors, incorporating more environmental factors does not significantly enhance the model's predictive ability. Our research facilitates the dynamic, continuous, and relatively accurate monitoring of GPP, contributing to a better understanding of carbon cycle dynamics and supporting informed ecosystem planning and management.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsDNR1 as a key regulator of rice resistance to 4-hydroxyphenylpyruvate dioxygenase-inhibiting herbicides. OsDNR1作为水稻抗4-羟基苯基丙酮酸双加氧酶除草剂的关键调控因子。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-06-27 DOI: 10.1111/jipb.13962

Yang Li, Xitie Ling, Wenting Zhang, Dongshu Guo, Jinyan Wang, Zeyu Qiu, Yuanda Lv, Yuwen Yang, Qing Liu, Xiaodong Hou, Baolong Zhang

引用次数: 0

GmGASA12 coordinates hormonal dynamics to enhance soybean water-soluble protein accumulation and seed size. GmGASA12调节激素动态，促进大豆水溶性蛋白积累和种子大小。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-06-24 DOI: 10.1111/jipb.13952

Yuming Yang, Lina Zhang, Huifang Zuo, Yifei Yang, Dandan Hu, Shanshan Zhang, Wenjie Yuan, Xuhao Zhai, Mengshi He, Mengjun Xu, Jinshe Wang, Weiguo Lu, Dezhou Hu, Deyue Yu, Fang Huang, Dan Zhang

{"title":"GmGASA12 coordinates hormonal dynamics to enhance soybean water-soluble protein accumulation and seed size.","authors":"Yuming Yang, Lina Zhang, Huifang Zuo, Yifei Yang, Dandan Hu, Shanshan Zhang, Wenjie Yuan, Xuhao Zhai, Mengshi He, Mengjun Xu, Jinshe Wang, Weiguo Lu, Dezhou Hu, Deyue Yu, Fang Huang, Dan Zhang","doi":"10.1111/jipb.13952","DOIUrl":"https://doi.org/10.1111/jipb.13952","url":null,"abstract":"Water-soluble protein (WSP) content determines soybean nutritional value and processing efficiency, yet its genetic and molecular regulation remains poorly understood. Here, we identified Glycine max gibberellic acid-stimulated Arabidopsis 12 (GmGASA12), encoding a gibberellin-regulated protein, as a major quantitative trait locus (QTL) governing WSP, through genome-wide association studies across five environments. Knockout of GmGASA12 resulted in 28.7% higher WSP content, 27.6% enlarged seed cells, and 20% yield increase, while overexpression suppressed these traits. Hormonal profiling revealed that GmGASA12 knockout elevates gibberellin, auxin, and abscisic acid but reduces cytokinin, driving cell expansion and protein body accumulation. GmGASA12 cooperatively regulates the biosynthesis of β-conglycinin and glycinin, the core storage proteins in soybean seeds, through its interaction with GmCG-6. Transcriptomics linked GmGASA12 to nitrogen metabolism and hormone signaling, with knockout upregulating amino acid transporters (GmAAP3/6/27) and storage protein genes (GmCG-1-6). Evolutionary analyses demonstrated strong selection for elite GmGASA12 haplotypes during domestication, with 94% of cultivars harboring favorable alleles. Our findings establish GmGASA12 as a molecular hub integrating hormonal dynamics and protein interaction to enhance soybean quality and yield, offering a pivotal target for breeding nutrient-dense varieties.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gaining insights into epigenetic memories through artificial intelligence and omics science in plants. 通过人工智能和植物组学获得表观遗传记忆的见解。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-06-24 DOI: 10.1111/jipb.13953

Judit Dobránszki, Valya Vassileva, Dolores R Agius, Panagiotis Nikolaou Moschou, Philippe Gallusci, Margot M J Berger, Dóra Farkas, Marcos Fernando Basso, Federico Martinelli

{"title":"Gaining insights into epigenetic memories through artificial intelligence and omics science in plants.","authors":"Judit Dobránszki, Valya Vassileva, Dolores R Agius, Panagiotis Nikolaou Moschou, Philippe Gallusci, Margot M J Berger, Dóra Farkas, Marcos Fernando Basso, Federico Martinelli","doi":"10.1111/jipb.13953","DOIUrl":"https://doi.org/10.1111/jipb.13953","url":null,"abstract":"Plants exhibit remarkable abilities to learn, communicate, memorize, and develop stimulus-dependent decision-making circuits. Unlike animals, plant memory is uniquely rooted in cellular, molecular, and biochemical networks, lacking specialized organs for these functions. Consequently, plants can effectively learn and respond to diverse challenges, becoming used to recurring signals. Artificial intelligence (AI) and machine learning (ML) represent the new frontiers of biological sciences, offering the potential to predict crop behavior under environmental stresses associated with climate change. Epigenetic mechanisms, serving as the foundational blueprints of plant memory, are crucial in regulating plant adaptation to environmental stimuli. They achieve this adaptation by modulating chromatin structure and accessibility, which contribute to gene expression regulation and allow plants to adapt dynamically to changing environmental conditions. In this review, we describe novel methods and approaches in AI and ML to elucidate how plant memory occurs in response to environmental stimuli and priming mechanisms. Furthermore, we explore innovative strategies exploiting transgenerational memory for plant breeding to develop crops resilient to multiple stresses. In this context, AI and ML can aid in integrating and analyzing epigenetic data of plant stress responses to optimize the training of the parental plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)P2 oscillation at the plasma membrane directs pollen germination atfh5标记的分泌囊泡与质膜上PI(4,5)P2振荡之间的相互调节指导花粉萌发。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2025-06-20 DOI: 10.1111/jipb.13945

Yuwan Zhao, Zijing Huang, Ting Wang, Yi Zhang, Zhufeng Chen, Yihao Li, Haiyun Ren

{"title":"Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)P2 oscillation at the plasma membrane directs pollen germination","authors":"Yuwan Zhao, Zijing Huang, Ting Wang, Yi Zhang, Zhufeng Chen, Yihao Li, Haiyun Ren","doi":"10.1111/jipb.13945","DOIUrl":"10.1111/jipb.13945","url":null,"abstract":"Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is known to be an instrumental anionic phospholipid in governing pollen germination and pollen tube growth. However, the precise functions and regulatory mechanisms of PI(4,5)P2 in pollen polarity establishment and germination remain poorly understood. Our previous studies demonstrated the pivotal involvement of Arabidopsis formin homology 5 (AtFH5)-dependent vesicle trafficking in polarity establishment of pollen. Here, we observed that PI(4,5)P2 accumulated and oscillated at the prospective germination site, a process closely associated with the rotational movement of AtFH5-labeled vesicles. Disruption of the mobility of AtFH5-labeled vesicles, either through AtFH5 mutation or pharmacological treatment, significantly perturbed the accumulation of PI(4,5)P2 at the plasma membrane. Subcellular localization and genetic analysis revealed that two phosphatidylinositol 4-phosphate 5-kinases, AtPIP5K1 and AtPIP5K4, are essential for PI(4,5)P2 oscillation at the germination site prior to pollen germination. Furthermore, we found that the dynamics of AtPIP5K4 depended on the mobility of AtFH5-labeled vesicles and reduced PI(4,5)P2 in turn disturbed the attachment of AtFH5-labeled secretory vesicles to the plasma membrane. In conclusion, these findings collectively highlight the reciprocal regulation of AtFH5-labeled secretory vesicles and PI(4,5)P2 oscillations at the plasma membrane, providing critical insights into the molecular mechanism underlying polarity establishment during pollen germination.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 8","pages":"2229-2244"},"PeriodicalIF":9.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13945","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0