Journal of Integrative Plant Biology最新文献_第3页

New perspective on pollen toxicity in Camellia oleifera 油茶花粉毒性的新视角。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-11-11 DOI: 10.1111/jipb.13803

Bin Yuan, Xiao-ming Fan, Fu-liang Hu, Yi-bo Luo

引用次数: 0

The regulatory network and critical factors promoting programmed cell death during embryogenesis. 胚胎发育过程中促进程序性细胞死亡的调控网络和关键因素。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-11-08 DOI: 10.1111/jipb.13795

An Luo, Ce Shi, Pan Luo, Zifu Zhao, Meng-Xiang Sun

{"title":"The regulatory network and critical factors promoting programmed cell death during embryogenesis.","authors":"An Luo, Ce Shi, Pan Luo, Zifu Zhao, Meng-Xiang Sun","doi":"10.1111/jipb.13795","DOIUrl":"https://doi.org/10.1111/jipb.13795","url":null,"abstract":"Programmed cell death (PCD) is essential for animal and plant development. However, the knowledge of the mechanism regulating PCD in plants remains limited, largely due to technical limitations. Previously, we determined that the protease NtCP14 could trigger PCD in the embryonic suspensor of tobacco (Nicotiana tabacum), providing a unique opportunity to overcome the limitations by creating synchronous two-celled proembryos with ongoing PCD for transcriptome analysis and regulatory factor screening. Here, we performed comparative transcriptome analysis using isolated two-celled proembryos and explored the potential regulatory network underlying NtCP14-triggered PCD. Multiple phytohormones, calcium, microtubule organization, the immunity system, soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins, long non-coding RNAs and alternative splicing are addressed as critical factors involved in the early stage of suspensor PCD. Genes thought to play crucial roles in suspensor PCD are highlighted. Notably, decreased antioxidant gene expression and increased reactive oxygen species (ROS) levels during suspensor PCD suggest a critical role for ROS signaling in the initiation of NtCP14-triggered PCD. Furthermore, five genes in the regulatory network are recommended as immediate downstream elements of NtCP14. Together, our analysis outlines an overall molecular network underlying protease-triggered PCD and provides a reliable database and valuable clues for targeting elements immediately downstream of NtCP14 to overcome technical bottlenecks and gain deep insight into the molecular mechanism regulating plant PCD.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc finger transcription factors BnaSTOP2s regulate sulfur metabolism and confer Sclerotinia sclerotiorum resistance in Brassica napus. 锌指转录因子 BnaSTOP2s 调节硫代谢并赋予甘蓝型油菜抗硬皮病的能力。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-11-06 DOI: 10.1111/jipb.13801

Lihong Dai, Zhaoqi Xie, Tianxu Ai, Yushun Jiao, Xiaoyi Lian, Angchen Long, Jinyun Zhang, Guangsheng Yang, Dengfeng Hong

{"title":"Zinc finger transcription factors BnaSTOP2s regulate sulfur metabolism and confer Sclerotinia sclerotiorum resistance in Brassica napus.","authors":"Lihong Dai, Zhaoqi Xie, Tianxu Ai, Yushun Jiao, Xiaoyi Lian, Angchen Long, Jinyun Zhang, Guangsheng Yang, Dengfeng Hong","doi":"10.1111/jipb.13801","DOIUrl":"10.1111/jipb.13801","url":null,"abstract":"Rapeseed (Brassica napus L.) exhibits high-sulfur requirements to achieve optimal growth, development, and pathogen resistance. Despite the importance of sulfur, the mechanisms regulating its metabolism and disease resistance are not fully understood. In this study, we found that the zinc finger transcription factors BnaSTOP2s play a pivotal role in sulfur metabolism and Sclerotinia sclerotiorum resistance. Our findings indicate that BnaSTOP2s are involved in sulfur metabolism, as evidenced by extensive protein interaction screening. BnaSTOP2s knockout reduced the content of essential sulfur-containing metabolites, including glucosinolate and glutathione, which is consistent with the significantly lowered transcriptional levels of BnaMYB28s and BnaGTR2s, key factors involved in glucosinolate synthesis and transportation, respectively. Comprehensive RNA-seq analysis revealed the substantial effect of BnaSTOP2s on sulfur metabolism from roots to siliques, which serve as pivotal sources and sinks for sulfur metabolism, respectively. Furthermore, we found that leaf lesion size significantly decreased and increased in the BnaSTOP2-OE and Bnastop2 mutants, respectively, compared with the wild-type during S. sclerotiorum infection, suggesting a vital role of BnaSTOP2s in plant defense response. In conclusion, BnaSTOP2s act as global regulators of sulfur metabolism and confer resistance to S. sclerotiorum infection in B. napus. Thus, they have potential implications for improving crop resilience.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recognition of a salivary effector by the TNL protein RCSP promotes effector-triggered immunity and systemic resistance in Nicotiana benthamiana. TNL 蛋白 RCSP 识别唾液效应物，促进效应物触发的免疫和烟草根的系统抗性。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-30 DOI: 10.1111/jipb.13800

Weiwei Rao, Tingting Ma, Jiayuan Cao, Yajun Zhang, Sisi Chen, Shu Lin, Xiaoxiao Liu, Guangcun He, Li Wan

{"title":"Recognition of a salivary effector by the TNL protein RCSP promotes effector-triggered immunity and systemic resistance in Nicotiana benthamiana.","authors":"Weiwei Rao, Tingting Ma, Jiayuan Cao, Yajun Zhang, Sisi Chen, Shu Lin, Xiaoxiao Liu, Guangcun He, Li Wan","doi":"10.1111/jipb.13800","DOIUrl":"https://doi.org/10.1111/jipb.13800","url":null,"abstract":"Insects secret chemosensory proteins (CSPs) into plant cells as potential effector proteins during feeding. The molecular mechanisms underlying how CSPs activate plant immunity remain largely unknown. We show that CSPs from six distinct insect orders induce dwarfism when overexpressed in Nicotiana benthamiana. Agrobacterium-mediated transient expression of Nilaparvata lugens CSP11 (NlCSP11) triggered cell death and plant dwarfism, both of which were dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), neuregulin 1 (NRG1) and SENESCENCE-ASSOCIATED GENE 101 (SAG101), indicating the activation of effector-triggered immunity (ETI) in N. benthamiana. Overexpression of NlCSP11 led to stronger systemic resistance against Pseudomonas syringae DC3000 lacking effector HopQ1-1 and tobacco mosaic virus, and induced higher accumulation of salicylic acid (SA) in uninfiltrated leaves compared to another effector XopQ that is recognized by a Toll-interleukin-1 receptor (TIR) domain nucleotide-binding leucine-rich repeat receptor (TNL) called ROQ1 in N. benthamiana. Consistently, NlCSP11-induced dwarfism and systemic resistance, but not cell death, were abolished in N. benthamiana transgenic line expressing the SA-degrading enzyme NahG. Through large-scale virus-induced gene silencing screening, we identified a TNL protein that mediates the recognition of CSPs (RCSP), including aphid effector MP10 that triggers resistance against aphids in N. benthamiana. Co-immunoprecipitation, bimolecular fluorescence complementation and AlphaFold2 prediction unveiled an interaction between NlCSP11 and RCSP. Interestingly, RCSP does not contain the conserved catalytic glutamic acid in the TIR domain, which is required for TNL function. Our findings point to enhanced ETI and systemic resistance by a TNL protein via hyperactivation of the SA pathway. Moreover, RCSP is the first TNL identified to recognize an insect effector.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MON1-CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice. MON1-CCZ1复合体在水稻自噬降解和液泡蛋白运输中发挥双重作用。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-30 DOI: 10.1111/jipb.13792

Binglei Zhang, Yihua Wang, Yun Zhu, Tian Pan, Haigang Yan, Xin Wang, Ruonan Jing, Hongming Wu, Fan Wang, Yu Zhang, Xiuhao Bao, Yongfei Wang, Pengcheng Zhang, Yu Chen, Erchao Duan, Xiaohang Han, Gexing Wan, Mengyuan Yan, Xiejun Sun, Cailin Lei, Zhijun Cheng, Zhichao Zhao, Ling Jiang, Yiqun Bao, Yulong Ren, Jianmin Wan

{"title":"The MON1-CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice.","authors":"Binglei Zhang, Yihua Wang, Yun Zhu, Tian Pan, Haigang Yan, Xin Wang, Ruonan Jing, Hongming Wu, Fan Wang, Yu Zhang, Xiuhao Bao, Yongfei Wang, Pengcheng Zhang, Yu Chen, Erchao Duan, Xiaohang Han, Gexing Wan, Mengyuan Yan, Xiejun Sun, Cailin Lei, Zhijun Cheng, Zhichao Zhao, Ling Jiang, Yiqun Bao, Yulong Ren, Jianmin Wan","doi":"10.1111/jipb.13792","DOIUrl":"https://doi.org/10.1111/jipb.13792","url":null,"abstract":"Autophagy is a highly conserved cellular program in eukaryotic cells which mediates the degradation of cytoplasmic components through the lysosome, also named the vacuole in plants. However, the molecular mechanisms underlying the fusion of autophagosomes with the vacuole remain unclear. Here, we report the functional characterization of a rice (Oryza sativa) mutant with defects in storage protein transport in endosperm cells and accumulation of numerous autophagosomes in root cells. Cytological and immunocytochemical experiments showed that this mutant exhibits a defect in the fusion between autophagosomes and vacuoles. The mutant harbors a loss-of-function mutation in the rice homolog of Arabidopsis thaliana MONENSIN SENSITIVITY1 (MON1). Biochemical and genetic evidence revealed a synergistic interaction between rice MON1 and AUTOPHAGY-RELATED 8a in maintaining normal growth and development. In addition, the rice mon1 mutant disrupted storage protein sorting to protein storage vacuoles. Furthermore, quantitative proteomics verified that the loss of MON1 function influenced diverse biological pathways including autophagy and vacuolar transport, thus decreasing the transport of autophagic and vacuolar cargoes to vacuoles. Together, our findings establish a molecular link between autophagy and vacuolar protein transport, and offer insights into the dual functions of the MON1-CCZ1 (CAFFEINE ZINC SENSITIVITY1) complex in plants.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsMAPK5–OsWRKY72 module negatively regulates grain length and grain weight in rice OsMAPK5-OsWRKY72 模块负向调节水稻的粒长和粒重。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-30 DOI: 10.1111/jipb.13786

Fuxiang Wang, Jiexin Lin, Fan Yang, Xiaofeng Chen, Yiyi Liu, Lingnan Yan, Jing Chen, Zonghua Wang, Huaan Xie, Jianfu Zhang, Huibin Xu, Songbiao Chen

{"title":"The OsMAPK5–OsWRKY72 module negatively regulates grain length and grain weight in rice","authors":"Fuxiang Wang, Jiexin Lin, Fan Yang, Xiaofeng Chen, Yiyi Liu, Lingnan Yan, Jing Chen, Zonghua Wang, Huaan Xie, Jianfu Zhang, Huibin Xu, Songbiao Chen","doi":"10.1111/jipb.13786","DOIUrl":"10.1111/jipb.13786","url":null,"abstract":"Grain size and grain weight are important determinants for grain yield. In this study, we identify a novel OsMAPK5–OsWRKY72 module that negatively regulates grain length and grain weight in rice. We found that loss-of-function of OsMAPK5 leads to larger cell size of the rice spikelet hulls and a significant increase in both grain length and grain weight in an indica variety Minghui 86 (MH86). OsMAPK5 interacts with OsMAPKK3/4/5 and OsWRKY72 and phosphorylates OsWRKY72 at T86 and S88. Similar to the osmapk5 MH86 mutants, the oswrky72 knockout MH86 mutants exhibited larger size of spikelet hull cells and increased grain length and grain weight, whereas the OsWRKY72-overexpression MH86 plants showed opposite phenotypes. OsWRKY72 targets the W-box motifs in the promoter of OsARF6, an auxin response factor involved in auxin signaling. Dual-luciferase reporter assays demonstrated that OsWRKY72 activates OsARF6 expression. The activation effect of the phosphorylation-mimicking OsWRKY72T86D/S88D on OsARF6 expression was significantly enhanced, whereas the effects of the OsWRKY72 phosphorylation-null mutants were significantly reduced. In addition, auxin levels in young panicles of the osmapk5 and oswrky72 mutants were significantly higher than that in the wild-type MH86. Collectively, our study uncovered novel connections of the OsMAPKK3/4/5-OsMAPK5-mediated MAPK signaling, OsWRKY72-mediated transcription regulation, and OsARF6-mediated auxin signaling pathways in regulating grain length and grain weight in an indica-type rice, providing promising targets for molecular breeding of rice varieties with high yield and quality.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2648-2663"},"PeriodicalIF":9.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than flowering: CONSTANS plays multifaceted roles in plant development and stress responses. 不仅仅是开花CONSTANS 在植物发育和应激反应中发挥着多方面的作用。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-28 DOI: 10.1111/jipb.13798

Bin Yu, Yilong Hu, Xingliang Hou

引用次数: 0

Big data and artificial intelligence-aided crop breeding: Progress and prospects. 大数据和人工智能辅助作物育种：进展与前景。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-28 DOI: 10.1111/jipb.13791

Wanchao Zhu, Weifu Li, Hongwei Zhang, Lin Li

{"title":"Big data and artificial intelligence-aided crop breeding: Progress and prospects.","authors":"Wanchao Zhu, Weifu Li, Hongwei Zhang, Lin Li","doi":"10.1111/jipb.13791","DOIUrl":"https://doi.org/10.1111/jipb.13791","url":null,"abstract":"The past decade has witnessed rapid developments in gene discovery, biological big data (BBD), artificial intelligence (AI)-aided technologies, and molecular breeding. These advancements are expected to accelerate crop breeding under the pressure of increasing demands for food. Here, we first summarize current breeding methods and discuss the need for new ways to support breeding efforts. Then, we review how to combine BBD and AI technologies for genetic dissection, exploring functional genes, predicting regulatory elements and functional domains, and phenotypic prediction. Finally, we propose the concept of intelligent precision design breeding (IPDB) driven by AI technology and offer ideas about how to implement IPDB. We hope that IPDB will enhance the predictability, efficiency, and cost of crop breeding compared with current technologies. As an example of IPDB, we explore the possibilities offered by CropGPT, which combines biological techniques, bioinformatics, and breeding art from breeders, and presents an open, shareable, and cooperative breeding system. IPDB provides integrated services and communication platforms for biologists, bioinformatics experts, germplasm resource specialists, breeders, dealers, and farmers, and should be well suited for future breeding.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplotype-resolved genome of a heterozygous wild peach reveals the PdaWRKY4-PdaCYP716A1 module mediates resistance to aphids by regulating betulin biosynthesis 杂合野生桃的单倍型基因组显示，PdaWRKY4-PdaCYP716A1模块通过调节甜菜素的生物合成来介导对蚜虫的抗性。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-25 DOI: 10.1111/jipb.13782

Jun-Xiu Wang, Yong Li, Xin-Wei Wang, Ke Cao, Chang-Wen Chen, Jin-Long Wu, Wei-Chao Fang, Geng-Rui Zhu, Xue-Jia Chen, Dan-Dan Guo, Jiao Wang, Ya-Lin Zhao, Jia-Qi Fan, Su-Ning Liu, Wen-Qing Li, Hang-Ling Bie, Qiang Xu, Li-Rong Wang

{"title":"Haplotype-resolved genome of a heterozygous wild peach reveals the PdaWRKY4-PdaCYP716A1 module mediates resistance to aphids by regulating betulin biosynthesis","authors":"Jun-Xiu Wang, Yong Li, Xin-Wei Wang, Ke Cao, Chang-Wen Chen, Jin-Long Wu, Wei-Chao Fang, Geng-Rui Zhu, Xue-Jia Chen, Dan-Dan Guo, Jiao Wang, Ya-Lin Zhao, Jia-Qi Fan, Su-Ning Liu, Wen-Qing Li, Hang-Ling Bie, Qiang Xu, Li-Rong Wang","doi":"10.1111/jipb.13782","DOIUrl":"10.1111/jipb.13782","url":null,"abstract":"<div>\u0000 \u0000 Wild species of domesticated crops provide valuable genetic resources for resistance breeding. Prunus davidiana, a wild relative of peach with high heterozygosity and diverse stress tolerance, exhibits high resistance against aphids. However, the highly heterozygous genome of P. davidiana makes determining the underlying factors influencing resistance traits challenging. Here, we present the 501.7 Mb haplotype-resolved genome assembly of P. davidiana. Genomic comparisons of the two haplotypes revealed 18,152 structural variations, 2,699 Pda_hap1-specific and 2,702 Pda_hap2-specific genes, and 1,118 allele-specific expressed genes. Genome composition indicated 4.1% of the P. davidiana genome was non-peach origin, out of which 94.5% was derived from almond. Based on the haplotype genome, the aphid resistance quantitative trait locus (QTL) was mapped at the end of Pda03. From the aphid resistance QTL, PdaWRKY4 was identified as the major dominant gene, with a 9-bp deletion in its promoter of the resistant phenotype. Specifically, PdaWRKY4 regulates aphid resistance by promoting PdaCYP716A1-mediated anti-aphid metabolite betulin biosynthesis. Moreover, we employed a genome design to develop a breeding workflow for rapidly and precisely producing aphid-resistant peaches. In conclusion, this study identifies a novel aphid resistance gene and provides insights into genome design for the development of resistant fruit cultivars.</div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2716-2735"},"PeriodicalIF":9.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miR396a–SlGRF8 module regulates sugar accumulation in the roots via SlSTP10 during the interaction between root-knot nematodes and tomato plants 在根结线虫与番茄植株的相互作用过程中，miR396a-SlGRF8 模块通过 SlSTP10 调节根部的糖积累。

IF 9.3 1区生物学

Journal of Integrative Plant Biology Pub Date : 2024-10-25 DOI: 10.1111/jipb.13794

Lulu Sun, Mengting Zhu, Xiaoxuan Zhou, Ruiyue Gu, Yuying Hou, Tongtong Li, Huang Huang, Rui Yang, Shaohui Wang, Wenchao Zhao

{"title":"The miR396a–SlGRF8 module regulates sugar accumulation in the roots via SlSTP10 during the interaction between root-knot nematodes and tomato plants","authors":"Lulu Sun, Mengting Zhu, Xiaoxuan Zhou, Ruiyue Gu, Yuying Hou, Tongtong Li, Huang Huang, Rui Yang, Shaohui Wang, Wenchao Zhao","doi":"10.1111/jipb.13794","DOIUrl":"10.1111/jipb.13794","url":null,"abstract":"<div>\u0000 \u0000 Root-knot nematodes (RKNs; Meloidogyne spp.) are a serious threat to crop production. The competition between plants and pathogens for assimilates influences the outcome of their interactions. However, the mechanisms by which plants and nematodes compete with each other for assimilates have not been elucidated. In this study, we demonstrated that miR396a plays a negative role in defense against RKNs and a positive role in sugar accumulation in tomato roots. The overexpression of SlGRF8 (Solanum lycopersicum growth-regulating factor 8), the target of miR396a, decreased the sugar content of the roots and the susceptibility to RKNs, whereas the grf8-cr mutation had the opposite effects. Furthermore, we confirmed that SlGRF8 regulated the sugar content in roots by directly activating the transcription of SlSTP10 (Solanum lycopersicum sugar transporter protein 10) in response to RKN stress. Moreover, SlSTP10 was expressed primarily in the tissues surrounding giant cells, and the SlSTP10 knockout increased both the sugar content in the roots and the plant's susceptibility to RKNs. Overall, this study provides important insight into the molecular mechanism through which the miR396a-SlGRF8-SlSTP10 module regulates sugar allocation in roots under RKN stress.</div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2701-2715"},"PeriodicalIF":9.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13794","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0